CN102965293A - Production of organophosphorus pesticide degrading bacterial agent by citric acid waste water - Google Patents
Production of organophosphorus pesticide degrading bacterial agent by citric acid waste water Download PDFInfo
- Publication number
- CN102965293A CN102965293A CN2011102566049A CN201110256604A CN102965293A CN 102965293 A CN102965293 A CN 102965293A CN 2011102566049 A CN2011102566049 A CN 2011102566049A CN 201110256604 A CN201110256604 A CN 201110256604A CN 102965293 A CN102965293 A CN 102965293A
- Authority
- CN
- China
- Prior art keywords
- citric acid
- liquid
- waste water
- organophosphorus pesticide
- bacterial agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a method for producing an organophosphorus pesticide degrading bacterial agent by citric acid waste water, and belongs to the technical fields of microbial agents and waste water resource recovery. The method mainly adopts high-concentration organic waste water produced in citric acid production process as a main culture medium raw material, and adopts a liquid fermentation method to produce a bacillus subtilis CGMCC No. 3374 bacterial agent. The production process comprises the following steps: (1) adjusting the pH of the citric acid waste water, performing sterilization; (2) inoculating the preserved bacillus subtilis on a slant, performing shaking culture to obtain liquid seeds; (3) inoculating the liquid seed in a fermentation tank filled with the citric acid waste water medium, performing sterile air culture to obtain a liquid fermentation product; (4) preparing a solid bacterial agent by using the fermentation product with bio-carbon as a carrier; (5) concentrating the fermented liquid, and adding a protective agent to obtain a liquid bacterial agent. The organophosphorus pesticide degrading bacterial agent produced by the process has the characteristics of low production cost and environment friendship, can degrade organophosphorus pesticide residues on plant surfaces and in the soil with high efficiency, can improve soil environment quality, and improve crop yield and quality. Meanwhile, an effective resource utilization method is provided for a lot of high-concentration waste water produced in citric acid production process, and discharge of pollutants is reduced.
Description
Technical field:
The invention belongs to microbiobacterial agent and waste water reclaiming technical field.
Background technology:
Citric acid is widely used in the industries such as food, medicine, chemical industry as a kind of important industrial chemicals and foodstuff additive.At present, in the nearly various schools of thinkers of state-owned citric acid production factory, about 800,000 tons of total annual capacity is maximum citric acid production state and export State of the whole world, the proportion of export volume in global citric acid total trade volume surpasses 60%.The production of citric acid be take potato do or corn as raw material, successively through raw material process, fermentation, extract, the operation such as refining makes product.Citric acid wastewater is mainly from the neutralizer, affination water, cleaning of evaporator water and the filter wash water distribution that ferment and abstraction process produces.Mainly contain starch, protein, carbohydrate, various organic acid, ammonia nitrogen and fat etc. in the waste water, COD concentration is 20000～30000mg/L, belongs to high concentrated organic wastewater.According to statistics, 1 ton of citric acid of every production produces 7.5～15 tons of high-concentration waste waters.Directly the discharging meeting will cause severe contamination to environment, and it is costly to process qualified discharge.The application technology as the second resource exploitation of citric acid wastewater is very urgent.
The characteristics such as organophosphorus pesticide is wide in variety with it, drug effect is high, prevention and treatment range wide spectrum, so far be still the maximum classification of consumption in the agricultural chemicals, play the important and pivotal role in agriculture production, the production of China's organophosphorus pesticide and usage quantity account for the over half of the total production of agricultural chemicals and usage quantity.But organophosphorus pesticide has stronger toxicity, and after long-term, a large amount of use, its residual meeting on farm crop causes food-safety problem, has also affected the agricultural products in China outlet, and the agricultural chemicals that is scattered also can make water and soil be polluted.Utilizing the residual biological renovation method of Microbial Degradation of Organophosphates to have the advantages such as low cost, nontoxic, non-secondary pollution, is one of the method for the reduction pesticidal contamination of tool application prospect.Chinese invention patent (application number: 200610090054.7) disclose the Pseudomonas stutzeri of a degrading organophosphorus pesticide, from insecticide factory's sewage draining exit pedotheque, separated; (application number: 200410081019.X) disclose the pale bacillus of a degrading organophosphorus pesticide, be to separate to obtain from the soil of long-term pesticidal contamination to Chinese invention patent.
This patent is the subtilis from the isolated high-efficiency degradation organophosphorus pesticide of rape phyllosphere, can keep good degradation property under the plant phyllospheric environment.The method of the main material production degradation of pesticide microbial inoculum take citric acid wastewater as substratum has no bibliographical information.This method not only can reduce the discharging of high concentrated organic wastewater, and reduces the production cost of microbial inoculum.
Summary of the invention:
The purpose of this invention is to provide a kind of method of can the recycling citric acid wastewater producing the organophosphorus pesticide degradation bacterial agent.
Bacterial strain used in the present invention is by the subtilis of contriver's separation screening high-efficiency degradation organophosphorus pesticide out (Bacillus subtilis) N14 bacterial strain, this bacterial strain is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 2nd, 2009, deposit number is: CGMCC No.3374, depositary institution address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
The production method of subtilis organophosphorus pesticide degradation bacterium of the present invention agent is as follows:
1, lemon acid waste water pretreatment: regulate the pH to 6.7 of waste water～7.8 with aqueous sodium hydroxide solution, in 115～126 ℃ of sterilizations 10～35 minutes.
2, liquid seeds preparation: the subtilis of inoculation slant preservation in the triangular flask that the good citric acid wastewater of pre-treatment is housed, in 28～45 ℃, 120～200 rev/mins of shaking culture 24～48 hours obtain liquid seeds.
3, liquid state fermentation: be 2%～20% liquid seeds to the inoculation volume ratio is housed in the good citric acid wastewater fermentor tank of pre-treatment, in 25～40 ℃, logical sterile air was cultivated 12-48 hour; Cultured fermented liquid is through count plate, and viable count reaches 5 * 10
8More than the CFU/ milliliter.
4, solid fungicide preparation: take the biological carbon of crossing 30 mesh sieves as carrier, according to fermented liquid: biological carbon is 1: 1.5～5 weight ratio, fermented liquid is added in the biological carbon, fully mix, obtain solid-state microbial inoculum, air seasoning is to microbial inoculum water content below 30%, and packing leaves shady and cool dry place in.
5, liquid bacterial agent preparation: cultured fermented liquid is after bacterial count, and ultrafiltration and concentration is adjusted to living bacteria count 5 * 10
9More than the CFU/mL, adding massfraction and be 0.5% glycine and massfraction is 1.5% glycerine, the can preservation;
Dilute with water 50-500 doubly during use.
Embodiment:
The subtilis microbiobacterial agent of being produced by technical solution of the present invention can the fast degradation plant leaf surface and soil in organophosphorus pesticide, and Promoting plant growth improves agricultural product quality, improves the farm environment quality; And production cost is low, has effectively utilized waste water, reduces pollutant emission.Embodiment is as follows:
Embodiment 1: citric acid wastewater is produced the subtilis microbiobacterial agent
Lemon acid waste water pretreatment: regulate wastewater pH to 7.0 with aqueous sodium hydroxide solution, in 121 ℃ of sterilizations 15 minutes.
Liquid seeds preparation: the subtilis of inoculation slant preservation in the triangular flask that the good citric acid wastewater of pre-treatment is housed, in 45 ℃, 150 rev/mins of shaking culture 36 hours obtain liquid seeds.
Liquid state fermentation: to the liquid seeds of inoculating 5% (volume ratio) in the good citric acid wastewater fermentor tank of pre-treatment is housed, in 32 ℃, logical sterile air was cultivated 16 hours; Cultured fermented liquid is through count plate, and viable count reaches 3.5 * 10
9The CFU/ milliliter.
Solid fungicide preparation: take the biological carbon of crossing 30 mesh sieves as carrier, according to fermented liquid: biological carbon is 1: 3 weight ratio, fermented liquid is added in the biological carbon, fully mix, obtain solid-state microbial inoculum, air seasoning is to microbial inoculum water content below 30%, packing, leave shady and cool dry place in, use in 12 months.
The liquid bacterial agent preparation: cultured fermented liquid is after bacterial count, and ultrafiltration and concentration is adjusted to living bacteria count 5 * 10
9More than the CFU/mL, adding massfraction and be 0.5% glycine and massfraction is 1.5% glycerine, can, and the room temperature preservation was used in 6 months.
Embodiment 2: the organophosphorus pesticide degradation bacterium agent is to the degradation effect of rape blade face SD-1750 pesticide residue
Choose two identical rape plot, each 30m
2, evenly spray 80% DDT EC agricultural chemicals of 1000 times of dilutions.Behind the 6h, evenly spray the degradation of pesticide liquid bacterial agent with 600 times of clear water dilutions, compare to spray clear water.Behind the 24h, it is residual that five point samplings detect rape blade face SD-1750, finds that treatment group reduces about 80% than control group SD-1750 residual quantity.
Embodiment 3: the organophosphorus pesticide degradation bacterium agent is to the degradation effect of Determination of Dimethoate Residues in the soil
Choose two identical farmland massifs of not planting plant, each 30m
2, evenly spray 50% dimethoate emulsifiable concentrate agricultural chemicals of 1000 times of dilutions; Behind the 6h, evenly be sprinkled into 1 kilogram of solid pesticide degradation bacterial agent, turn over evenly; Behind the 36d, five point samplings detect the residual of rape blade face Rogor, find that treatment group reduces about 50% than control group Determination of Dimethoate Residues amount.
Embodiment 4: the subtilis microbiobacterial agent promotes growth of rape
Adopt the experiment of green house of vegetables residential quarter, 8 experiment residential quarters that conditionally complete is identical are set, each residential quarter 30m
2, 2 residential quarters are 1 group of parallel test, are divided into into 4 groups, the plantation rape, and in the Brassica Napus Seedling phase, do not apply fertilizer and compare in the 1st group of 2 residential quarters; The 2nd group of 2 residential quarter are respectively imposing the subtilis microbiobacterial agent once according to 50 kg/acres consumption behind the rape complete germination He after 3 weeks; Urea is respectively being used once according to 15 kg/acres rate of fertilizer application behind the rape complete germination He after 3 weeks in the 3rd group of 2 residential quarters; The 4th group 2 rates of fertilizer application according to 15 kg/acres are respectively being used urea once behind the rape complete germination He after 3 weeks, and impose microbiobacterial agent once, the irrigation that each residential quarter is identical and way to manage according to 50 kg/acres rate of fertilizer application in 1 day after using urea at every turn.Growth of rape 5 all " Invest, Then Investigate " yield and qualities.Use separately microbiobacterial agent and use separately the yield of rape of urea basically identical, all than the check plot volume increase about 60% of not applying fertilizer; Contrast residential quarter, the residential quarter volume increase about 90% that two kinds of microbial inoculums and urea are all used; Nitrate and nitrite content be not to apply fertilizer and to use separately the minimum of microbiobacterial agent in the rape, in below 1/3 of national standard Limited Doses, use use again behind the urea microbiobacterial agent also national standard limit the quantity of below 1/2, nitrate and nitrite content all exceed standard seriously in the rape of urea and use separately.Show that this microbiobacterial agent has good effect of increasing production, and can obviously improve the quality of rape.
The bacterial strain of degrading organic phosphor pesticides provided by the present invention is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preservation date is on November 2nd, 2009, and Classification And Nomenclature is subtilis (Bacillus subtilis).Deposit number is: CGMCC No.3374.
Claims (2)
1. method of utilizing citric acid wastewater to produce the organophosphorus pesticide degradation bacterium agent, it is characterized in that: organophosphorus pesticide degradation bacterium is subtilis (Bacillus subtilis) N14, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number: CGMCC No.3374.
2. utilize the production method of the described organophosphorus pesticide degradation bacterium agent of claim 1, it is characterized in that: fermention medium is take citric acid wastewater as main raw material, and microbial inoculum production method and step comprise:
(1) lemon acid waste water pretreatment: regulate the pH to 6.7 of waste water～7.8 with aqueous sodium hydroxide solution, in 115～126 ℃ of sterilizations 10～35 minutes.
(2) liquid seeds preparation: the subtilis of inoculation slant preservation in the triangular flask that the good citric acid wastewater of pre-treatment is housed, in 28～45 ℃, 120～200 rev/mins of shaking culture 24～48 hours obtain liquid seeds.
(3) liquid state fermentation: be 2%～20% liquid seeds to the inoculation volume ratio is housed in the good citric acid wastewater fermentor tank of pre-treatment, in 25～40 ℃, logical sterile air was cultivated 12-48 hour; Cultured fermented liquid is through count plate, and viable count reaches 5 * 10
8More than the CFU/ milliliter.
(4) solid fungicide preparation: take the biological carbon of crossing 30 mesh sieves as carrier, according to fermented liquid: biological carbon is 1: 1.5～5 weight ratio, fermented liquid is added in the biological carbon, fully mix, obtain solid-state microbial inoculum, air seasoning is to microbial inoculum water content below 30%, and packing leaves shady and cool dry place in.
(5) liquid bacterial agent preparation: cultured fermented liquid is after bacterial count, and ultrafiltration and concentration is adjusted to living bacteria count 5 * 10
9More than the CFU/mL, adding massfraction and be 0.5% glycine and massfraction is 1.5% glycerine, the can preservation; Dilute with water 50-500 doubly during use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102566049A CN102965293A (en) | 2011-09-01 | 2011-09-01 | Production of organophosphorus pesticide degrading bacterial agent by citric acid waste water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102566049A CN102965293A (en) | 2011-09-01 | 2011-09-01 | Production of organophosphorus pesticide degrading bacterial agent by citric acid waste water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102965293A true CN102965293A (en) | 2013-03-13 |
Family
ID=47795729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102566049A Pending CN102965293A (en) | 2011-09-01 | 2011-09-01 | Production of organophosphorus pesticide degrading bacterial agent by citric acid waste water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102965293A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106957804A (en) * | 2016-12-28 | 2017-07-18 | 中国科学院生态环境研究中心 | Prevent and treat the microbe controlling method that drip emitter is blocked |
| CN108275841A (en) * | 2018-02-24 | 2018-07-13 | 昆明滇俊环保除臭技术有限公司 | A kind of fertile technique of traditional Chinese medicine wastewater recycling system |
| CN110105136A (en) * | 2019-05-08 | 2019-08-09 | 石河子大学 | A kind of citric acid microbial bacterial agent and its preparation method and application |
| CN111849812A (en) * | 2020-07-17 | 2020-10-30 | 众合发(北京)生物科技发展有限公司 | Compound for degrading organophosphorus pesticide residues in water body and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709282A (en) * | 2009-12-23 | 2010-05-19 | 河北科技大学 | Organophosphorus pesticide degrading bacterium and method for producing microbial inoculum thereof |
| CN101724596A (en) * | 2010-01-11 | 2010-06-09 | 北京联合大学 | Method for culturing organophosphorus pesticide degrading bacteria |
| CN102127513A (en) * | 2010-01-12 | 2011-07-20 | 北京联合大学 | Method for culturing Bacillus subtilis by using potato starch wastewater |
-
2011
- 2011-09-01 CN CN2011102566049A patent/CN102965293A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709282A (en) * | 2009-12-23 | 2010-05-19 | 河北科技大学 | Organophosphorus pesticide degrading bacterium and method for producing microbial inoculum thereof |
| CN101724596A (en) * | 2010-01-11 | 2010-06-09 | 北京联合大学 | Method for culturing organophosphorus pesticide degrading bacteria |
| CN102127513A (en) * | 2010-01-12 | 2011-07-20 | 北京联合大学 | Method for culturing Bacillus subtilis by using potato starch wastewater |
Non-Patent Citations (2)
| Title |
|---|
| 《中国农学通报》 20110825 盛萱宜 等 "利用甘薯淀粉废水生产有机磷农药降解菌剂条件的初探" 第259-265页 1-2 第27卷, 第21期 * |
| 盛萱宜 等: ""利用甘薯淀粉废水生产有机磷农药降解菌剂条件的初探"", 《中国农学通报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106957804A (en) * | 2016-12-28 | 2017-07-18 | 中国科学院生态环境研究中心 | Prevent and treat the microbe controlling method that drip emitter is blocked |
| CN108275841A (en) * | 2018-02-24 | 2018-07-13 | 昆明滇俊环保除臭技术有限公司 | A kind of fertile technique of traditional Chinese medicine wastewater recycling system |
| CN110105136A (en) * | 2019-05-08 | 2019-08-09 | 石河子大学 | A kind of citric acid microbial bacterial agent and its preparation method and application |
| CN111849812A (en) * | 2020-07-17 | 2020-10-30 | 众合发(北京)生物科技发展有限公司 | Compound for degrading organophosphorus pesticide residues in water body and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106947725B (en) | Microbial agent and application thereof in saline-alkali soil improvement | |
| CN103172437B (en) | Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof | |
| CN103204732B (en) | Composite microbial fertilizer containing polyglutamate high-producing strain, preparation method and application thereof | |
| CN102976801B (en) | Method for producing functional microorganism organic fertilizer by using food residue | |
| CN103820348B (en) | The production method of one strain plant growth-promoting bacteria and microbial inoculum thereof and application | |
| CN103820349B (en) | One bacillus licheniformis and the production method of microbial inoculum thereof and purposes | |
| CN105294284A (en) | Preparation method of straw fermented biological fertilizer | |
| CN103467185A (en) | Biological compound organic bacterial fertilizer | |
| CN103820350B (en) | Changing food waste into resources produces bacillus amyloliquefaciens microbial fertilizer | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN102391876A (en) | Composite biological soil modifier and application thereof | |
| CN108865161B (en) | Composite saline-alkali soil modifier and preparation and application thereof | |
| CN102249753A (en) | Method for producing multifunctional bioorganic fertilizer and application of multifunctional bioorganic fertilizer | |
| CN105130685A (en) | Preparation method for organic fertilizer containing Nu-polyglutamic acid | |
| CN107266249A (en) | A kind of prevention and control vegetable insect disease type double membrane slow release fertilizer and preparation method thereof | |
| CN104480097A (en) | Green alga glycosyl microcapsule biological agent and preparation method and application thereof | |
| CN102249754A (en) | Paenibacillus polymyxa microbial fertilizer produced through sweet potato starch waste water recovery | |
| CN109438100A (en) | A kind of preparation method of the dedicated stress-resistant seaweed water-soluble fertilizer of fruits and vegetables | |
| CN102180710B (en) | Method for producing rhodobacter sphaeroides microbial fertilizer from citric acid waste water | |
| CN103931660A (en) | Method utilizing critic acid wastewater to produce bacillus amyloliquefaciens bio-control inoculant | |
| CN102965293A (en) | Production of organophosphorus pesticide degrading bacterial agent by citric acid waste water | |
| CN102618527A (en) | Method for recycling production of paenibacillus polymyxa microbial agent from citric acid wastewater | |
| CN1916155B (en) | Method for preparing fermention medium, and producing biological pesticide by using wastewater from fermentation | |
| CN102533594B (en) | Fermentation culture of Bacillus vallismortis and production method of compound microbial fertilizer | |
| CN104016733A (en) | Multifunctional bio-organic fertilizer produced by utilizing resource recovery of yeast wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130313 |