CN102960581B - Composite premix for controlling alcohol positive milk, and preparation method and application of thereof - Google Patents
Composite premix for controlling alcohol positive milk, and preparation method and application of thereof Download PDFInfo
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- CN102960581B CN102960581B CN201210556560.6A CN201210556560A CN102960581B CN 102960581 B CN102960581 B CN 102960581B CN 201210556560 A CN201210556560 A CN 201210556560A CN 102960581 B CN102960581 B CN 102960581B
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Abstract
The invention discloses a composite premix for controlling alcohol positive milk, and a preparation method and an application thereof. The composite premix comprises the following raw materials in weight percentage: 37 to 47% of sodium citrate, 11 to 21% of sodium propionate, 11 to 21% of potassium iodide, and 21 to 31% of yeast cell wall polysaccharide. The composite premix for controlling alcohol positive milk, prepared by the preparation method disclosed by the invention, can effectively adjust and control the ion metabolism in the dairy cattle, so that the alcohol positive milk can be effectively controlled; and the composite premix can be massively applied to industrial production and actual operation.
Description
Technical field
The present invention relates to a kind of compound premix of preventing and treating alcohol positive milk of cow and its preparation method and application.
Background technology
Alcohol positive milk refers to the general name of mixing the cow's milk of the tiny cotton-shaped grumeleuse producing under normal temperature with the alcohol of 68-70% and the milk of equivalent.Because the heat endurance of alcohol positive milk is poor, in the time that temperature exceedes 120 DEG C, this breast easily solidifies and blocks pipeline, brings difficulty to Dairy Processing.Therefore, Dairy Processing enterprise, in the time of purchase sweet milk, all carries out alcohol positive milk inspection, just rejects once be positive, and brings massive losses to raiser, causes the serious wasting of resources simultaneously.
Alcohol positive milk can be divided into two kinds of high acidity alcohol test positive milk and low acidity alcohol test positive milk.High acidity alcohol test positive milk refers to that titratable acidity is higher than 18 degree, mixes with equivalent 68-70% alcohol the breast solidifying.This breast is mainly due to reasons such as the equipment disinfections such as milking pipeline are tight, pasture environmental sanitation is bad, milk keeping method is improper, makes bacterium amount reproduction, growth, and lactose resolves into lactic acid, and lactic acid raises, due to protein denaturation.Low acidity alcohol test positive milk refers to that titratable acidity is between 11-18 degree, and lactic acid content is not high, mixes with equivalent 68-70% alcohol the breast solidifying.This breast is similar to normal lactic acid degree, and dry, crude protein, fat, amino acid, pH value and the normal basic indistinction of milk, causes mainly due to Lactation of Dairy Cow physiologic derangement.Conventionally the said alcohol positive milk of people generally refers to low acidity alcohol test positive milk.
The genesis mechanism of alcohol positive milk of cow is extremely complicated, relates to a series of physiological regulating control processes such as Lactation of Dairy Cow, endocrine and immunity, not yet finds the absolute factor that alcohol positive milk occurs at present.Comprehensive lot of documents, we think that the generation of alcohol positive milk may be relevant with following 3 factors:
One, the ionic equilibrium of cow's milk gets muddled.Balance in milk elements between calcium, phosphorus, magnesium, phosphate, citrate is to ensure the necessary condition of the stability of milk, if wherein any content too much all can affect the change of stability of milk.Large quantity research shows, alcohol positive Ruzhong Ca
2+, Mg
2+content raises, and has neutralized the surface charge of casein protomere, causes its space conformation unstable, under alcoholic dehydration, loses hydration shell, finally forms colloid aggegation.(Yang Hongjun, Li Qingguan, Wang Chunao. the research [J] that the sick bovine blood of alcohol positive milk changes. Shandong animal and veterinary, 2004 (2): 1-2.; Li Lingyan, Wei Yong, Li Shengli. the positive milk composition of variable concentrations alcohol titration and content of mineral substances comparative studies [J] thereof. Chinese milk cow, 2010,9:49-52.)
Two, milk cow endocrine metabolism imbalance.When hormone is promoted the sexual maturity in cow oestrus phase, latter half of gestation, ovarian cyst and injection, easily cause hormonal change.Due to estrogenic effect, the permeability of uterus, mammary gland capillary is obstructed, the increased content of calcium in milk, easily produces alcohol positive milk.Generally can take intramuscular injection HCG or Progesterone Treatment, illustrate that hormonal readiness changes relevant to the generation of alcohol positive milk.(Li Jing, Liu Deyi, Zhou Yan, Shang Changfa. the pathogenetic progress of alcohol positive milk of cow [J]. Jiangsu agricultural sciences, 2011,39 (2): 336-338).
Three, milk cow mammary glandular cell metabolic disorder.Regulate the variation of second messenger's cyclic adenosine monophosphate (cAMP) of mammary gland metabolism and the concentration of cyclic guanylic acid (cGMP) directly to reflect the change of mammary cell metabolism.Correlative study shows, alcohol positive Ruzhong cAMP rising, cGMP decline, cAMP/cGMP rising.Due to what therefore, we thought alcohol positive milk is Cell regulate horizontal abnormality.(Liu Li, Zhu Yongning, Xue Yongsan. the mensuration [J] of alcohol positive Ruzhong cyclic adenosine monophosphate and cyclic guanylic acid content. Chinese animal doctor's science and technology, 2004,34 (6): 50-52.)
At present, there is no the method for special efficacy for the control of alcohol positive milk of cow.Generally there is clinically following several therapeutic scheme, be mainly: (1) sodium dihydrogen phosphate 40-70g, 1 time for oral administration, every day 1 time, serve on 7-10 days.(2) 2% methyl mercapto urea pyrimidine 20m1,1 intramuscular injection.(3) regulate mammary gland capillary permeability, can intramuscular injection vitamin C.(4) there is alcohol positive milk when cow oestrus, can intramuscular injection progesterone.But, for above-mentioned several results for the treatment of of preventing and treating scheme, also there is no definite clinical data at present, the above-mentioned method of preventing and treating does not apply in actual production veritably yet.
In sum, alcohol positive milk is a kind of symptom, and its reason is diversified.At present, generally all remove to treat alcohol positive milk of cow by following 2 strategies aborning, be respectively: (1) regulates the metabolism of milk cow sodium ion; (2) improve cow breast health status.Above two kinds of strategies have certain effect clinically, but owing to there is no to consider the Different types of etiopathogenises of alcohol positive milk comprehensively, and its effect is limited or even invalid often.This phenomenon is urgently to be resolved hurrily.
Summary of the invention
Technical problem to be solved by this invention is to overcome existing alcohol positive milk, and to prevent and treat method effect limited, and cannot large-scale application arrive the medium many defects of actual production, a kind of compound premix of preventing and treating alcohol positive milk of cow and its preparation method and application is provided.The compound premix of this control alcohol positive milk of cow can effectively regulate the ion metabolism in Contents in Cows, strengthen the immune performance of milk cow, thereby reach the effect of high-efficiency prevention and control alcohol positive milk of cow, and can be applied on a large scale in industrial production and practical operation.
The present invention, fill up the blank of prior art, the present invention is on the basis in conjunction with existing control methods and strategies, from the pathogenesis of alcohol positive milk, by add the method for compound premix in feed, regulate the metabolism of milk cow calcium ion, sodium ion metabolism, negative energy balance and breast immunity, reach the object for the treatment of alcohol positive milk of cow.
One of object of the present invention is, a kind of compound premix of preventing and treating alcohol positive milk of cow is provided, and its composition comprises: the KI of the natrium citricum of 37%-47%, the sodium propionate of 11%-21%, 11%-21% and the yeast cell wall polysaccharide of 21%-31%; Described percentage is the percentage by weight that accounts for raw material.
Preferably, the compound premix of described control alcohol positive milk of cow consists of the following composition: the KI of the natrium citricum of 37%-47%, the sodium propionate of 11%-21%, 11%-21% and the yeast cell wall polysaccharide of 21%-31%; Described percentage is the percentage by weight that accounts for raw material.
In the present invention, described yeast cell wall polysaccharide is the yeast cell wall polysaccharide of this area routine; Preferably, the Main Ingredients and Appearance of described yeast cell wall polysaccharide is glucan and mannosan; Better, described commercially available the obtaining of yeast cell wall polysaccharide, it buys producer is Qingdao Hai Run moral Bioisystech Co., Ltd, product " raw power element " by name.
Preferably, the addition of described natrium citricum is 39%-45%; The addition of described sodium propionate is 13%-19%, and the addition of described KI is 13%-19%, and the addition of described yeast cell wall polysaccharide is 23%-29%; Described percentage is the percentage by weight that accounts for raw material.
Better, the addition of described natrium citricum is 42%; The addition of described sodium propionate is 16%, and the addition of described KI is 16%, and the addition of described yeast cell wall polysaccharide is 26%; Described percentage is the percentage by weight that accounts for raw material.
In compound premix of the present invention, also can contain conventional various other additives that add in the compound premix of this area, as long as it does not significantly affect the effect of compound premix of the present invention.
Two of object of the present invention is, the preparation method of the compound premix of above-mentioned control alcohol positive milk of cow is provided, and its step comprises: the compound premix of above-mentioned control alcohol positive milk of cow is mixed.
Three of object of the present invention is to provide the compound premix of above-mentioned control alcohol positive milk of cow in the application of preparing in milk cow forage.
Preferably, the compound premix of control alcohol positive milk of cow of the present invention is added in milk cow TRM, mix; Better, the addition of the compound premix of described control alcohol positive milk of cow is 50-100 gram in every cow head feed every day; Further better, the addition of the compound premix of described control alcohol positive milk of cow is in every cow head feed every day 70 grams.
Meeting on the basis of this area general knowledge, above-mentioned each optimum condition, can be combined, and obtains the preferred embodiments of the invention.
Agents useful for same of the present invention and raw material be commercially available obtaining all.
Positive progressive effect of the present invention is: the ion metabolism of the compound premix of the control alcohol positive milk of cow that the present invention makes in can Effective Regulation Contents in Cows, thereby high-efficiency prevention and control alcohol positive milk of cow, and can be applied on a large scale in industrial production and practical operation.
Detailed description of the invention
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example, according to conventional method and condition, or selects according to catalogue.
Embodiment 1-8
Raw material and the content thereof of the compound premix of embodiment 1-8 are as shown in table 1.
Raw material and the content thereof of the made compound premix of table 1 the present invention
Described percentage is the percentage by weight that accounts for raw material;
Described yeast cell wall polysaccharide is produced by Qingdao Hai Run moral Bioisystech Co., Ltd, product " raw power element " by name.
The preparation method of the compound premix of embodiment 1-8 is: above-mentioned raw materials is mixed.
Embodiment 1-8: milk cow is fed the conventional feed of the compound premix that has added control alcohol positive milk of cow of the present invention; Conventional feed is made up of concentrate supplement and coarse fodder, and wherein concentrate supplement is " material in early days " that Guangming Hesitan Livestocks Co., Ltd. Shanghai produces, and coarse fodder comprises one or more in silage corn, clover and sheep's hay.
In the milk cow forage of embodiment 1-8, the addition of premix is:
Embodiment 1,2: every day 50 grams of every cow heads; Embodiment 3,4: every day 100 grams of every cow heads; Embodiment 5-8: every day 70 grams of every cow heads;
Comparative example 1
Milk cow is fed conventional full mixed fodder, does not contain the compound premix of control alcohol positive milk of cow of the present invention.
Effect embodiment 1
For the compound premix of probing into the control alcohol positive milk of cow that the present invention the makes effect in control alcohol positive milk of cow, the present invention has carried out feeding experiment.Test select parity (2 tires or 3 tires) close, suffer from 90 of oxen in lactation early stage (being less than 15 days), alcohol positive milk that the output of milk is close.Be divided at random 9 groups, every group of 10 ill milk cows, wherein, group 1 to group 8 is fed respectively the feed that has added the compound premix in embodiment 1-8; Group 9 is for feeding the feed in comparative example 1.Feed consumption is substantially suitable, and result of the test is in table 2, table 3 and table 4:
The impact of the different embodiment of table 2 on alcohol positive milk of cow curative effect
As shown in Table 2, the compound premix of control alcohol positive milk of cow of the present invention can effectively be prevented and treated alcohol positive milk.The feed of the compound premix that contains control alcohol positive milk of cow of the present invention when feeding is after 1 week, and group 9 only has 20% trouble ox to transfer feminine gender to, and group 1-8 suffers from the recessive conversion ratio of ox all higher than group 9; In the time feeding patented product 2 weeks, group 5 has 90% trouble ox to transfer feminine gender to, and control group only has 60% to transfer feminine gender to; In the time feeding patented product 1 month, group 2 and group 5-8 all transfer feminine gender to, and group 9 still has 20% trouble ox.
The different embodiment of table 3 are to cow blood and Ruzhong calcium ion (Ca
2+) impact of concentration
Note: the different significant differences (P<0.05) that represent of same row shoulder motes capitalization, lower same.
As shown in Table 3, the compound premix energy Effective Regulation cow blood of control alcohol positive milk of cow of the present invention and the calcium ion metabolism in Ruzhong.When the compound premix of the control alcohol positive milk of cow that the present invention that feeds makes is after 2 weeks, the calcium ion concentration in cow blood and Ruzhong all has a declining tendency, wherein organize calcium ion concentration in 5-7 cow blood significantly lower than group 9(P<0.05), group 5 milk cow emulsion calcium ion concentrations are also significantly lower than group 9(P<0.05).When the compound premix of the control alcohol positive milk of cow that the present invention that feeds makes is after 4 weeks, milk cow Ruzhong calcium ion concentration has downward trend, but not significantly (P>0.05) of difference; Group 5 and group 6 cow blood calcium ion concentrations are significantly lower than group 9(P<0.05).
The different embodiment of table 4 are to cow blood and Ruzhong sodium ion (Na
+) impact of concentration
As shown in Table 4, the compound premix energy Effective Regulation cow blood of control alcohol positive milk of cow of the present invention and the sodium ion metabolism in Ruzhong.The compound premix of the control alcohol positive milk of cow that the present invention that feeds makes is after 2 weeks, the Na ion concentration in cow blood and Ruzhong has the trend of rising, wherein organizes 5 and is significantly higher than group 9(P<0.05 with the Na ion concentration of organizing in 7 milk cow emulsions).The compound premix of the control alcohol positive milk of cow that the present invention that feeds makes is after 4 weeks, the Na ion concentration in cow blood and Ruzhong also has the trend of rising, wherein organizes 5 and is significantly higher than group 9(P<0.05 with the Na ion concentration of organizing in 6 milk cow emulsions).
Milk is a kind of stable colloid system, and its stability is main relevant with casein wherein.Normal cow's milk, casein seldom exists with the form of monomer, but is scattered in whey with the form of protomere, and for alcohol positive milk, because the ionic equilibrium in its Ruzhong is destroyed, therefore this newborn stability sharply declines.Correlative study shows, different from normal breast, sodium ion and the potassium content in alcohol positive Ruzhong significantly reduce, and calcium ion and magnesium ion content significantly increase.(Li Lingyan, Wei Yong, Li Shengli. the positive milk composition of variable concentrations alcohol titration and content of mineral substances comparative studies [J] thereof. Chinese milk cow, 2010, (9): 49-52).
By effect, embodiment shows, the compound premix of the control alcohol positive milk of cow that the present invention makes not only can significantly improve the sodium ions content in cow blood and Ruzhong, can also significantly reduce the calcium ion content in cow blood and Ruzhong.Therefore, product of the present invention can fundamentally be prevented and treated the generation of alcohol positive milk of cow.
The compound premix of the control alcohol positive milk of cow that the present invention makes, to add in milk cow TRM with the form of compound premix, the method also has significant effect to the generation of prevention alcohol positive milk of cow,, no matter whether milk cow is ill, the compound premix that can add the present invention to make in the daily feed of milk cow, thus reach the object of generation of prevention alcohol positive milk of cow.
Claims (8)
1. a compound premix of preventing and treating alcohol positive milk of cow, is characterized in that: it consists of the following composition: the KI of the natrium citricum of 39%-45%, the sodium propionate of 13%-19%, 13%-19% and the yeast cell wall polysaccharide of 23%-29%; Described percentage is the percentage by weight that accounts for raw material.
2. the compound premix of control alcohol positive milk of cow as claimed in claim 1, is characterized in that: the Main Ingredients and Appearance of described yeast cell wall polysaccharide is glucan and mannosan.
3. the compound premix of control alcohol positive milk of cow as claimed in claim 1, is characterized in that: described commercially available the obtaining of yeast cell wall polysaccharide, it buys producer is Qingdao Hai Run moral Bioisystech Co., Ltd, the ProductName power element of making a living.
4. the compound premix of control alcohol positive milk of cow as claimed in claim 1, is characterized in that: the addition of described natrium citricum is 42%; The addition of described sodium propionate is 16%, and the addition of described KI is 16%, and the addition of described yeast cell wall polysaccharide is 26%; Described percentage is the percentage by weight that accounts for raw material.
5. a preparation method for the compound premix of the control alcohol positive milk of cow described in any one in claim 1-4, is characterized in that: its step comprises: the compound premix of the control alcohol positive milk of cow as described in any one in claim 1-4 is mixed.
6. the compound premix of the control alcohol positive milk of cow as described in any one in claim 1-4 is in the application of preparing in milk cow forage.
7. the compound premix of control alcohol positive milk of cow as claimed in claim 6, in the application of preparing in milk cow forage, is characterized in that: the addition of the compound premix of described control alcohol positive milk of cow is 50-100 gram in every cow head feed every day.
8. the compound premix of control alcohol positive milk of cow as claimed in claim 7, in the application of preparing in milk cow forage, is characterized in that: the addition of the compound premix of described control alcohol positive milk of cow is in every cow head feed every day 70 grams.
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| CN103859235B (en) * | 2014-04-02 | 2015-07-01 | 山西农业大学 | Licking agent for preventing low-acidity alcohol positive milk of cow and preparation method thereof |
| CN111296357B (en) * | 2020-01-04 | 2022-05-27 | 邹平百航农牧科技有限公司 | Three-stage feeding method for weaned piglets and special feed for three-stage feeding method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101732344A (en) * | 2009-12-25 | 2010-06-16 | 北京农业职业学院 | Preparation for treating alcohol positive milk of dairy cow |
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| CN101732344A (en) * | 2009-12-25 | 2010-06-16 | 北京农业职业学院 | Preparation for treating alcohol positive milk of dairy cow |
Non-Patent Citations (10)
| Title |
|---|
| 低酸度酒精阳性乳的治疗和预防;刘东军;《兽医导刊》;20101215(第12期);第30页左栏第2段，第31页二、治疗措施 * |
| 刘东军.低酸度酒精阳性乳的治疗和预防.《兽医导刊》.2010,(第12期), |
| 史长明.奶牛酒精阳性乳的防治.《农家参谋》.2007,(第08期), |
| 奶牛酒精阳性乳的防治;史长明;《农家参谋》;20070805(第08期);全文 * |
| 奶牛酒精阳性乳要多措并举综合防治;崔晨光等;《今日畜牧兽医》;20110630(第S1期);全文 * |
| 崔晨光等.奶牛酒精阳性乳要多措并举综合防治.《今日畜牧兽医》.2011,(第S1期), |
| 彭小芳等.酵母细胞壁多糖的生理功能及其应用研究进展.《湖南饲料》.2011,(第05期), |
| 朱春森等.酵母细胞壁多糖的研究进展.《江西饲料》.2007,(第04期), |
| 酵母细胞壁多糖的生理功能及其应用研究进展;彭小芳等;《湖南饲料》;20110928(第05期);全文 * |
| 酵母细胞壁多糖的研究进展;朱春森等;《江西饲料》;20070828(第04期);全文 * |
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