CN102960181A - Operation method for improving production inoculation uniformity of liquid strains - Google Patents
Operation method for improving production inoculation uniformity of liquid strains Download PDFInfo
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- CN102960181A CN102960181A CN2012103163393A CN201210316339A CN102960181A CN 102960181 A CN102960181 A CN 102960181A CN 2012103163393 A CN2012103163393 A CN 2012103163393A CN 201210316339 A CN201210316339 A CN 201210316339A CN 102960181 A CN102960181 A CN 102960181A
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Abstract
The invention relates to an inoculation operation method, and belongs to the field of biological engineering. The operation method for improving production inoculation uniformity of liquid strains comprises the following step of: carrying out inoculation on multiple cultivation bottles with inoculation equipmentliquid strains, wherein the inoculation equipment comprises a seed temporary storage devices and a spraying device, and a plurality of sprayers are formed on the spraying device. A One spraying device is communicated with at least two seed temporary storage devices, and one cultivation bottle is arranged below each sprayer. A seeding tank is arranged; the seeding tank is communicated with at least two seed temporary storage devices through a peristaltic pump; and the peristaltic pump fills the liquid strains in the seeding tank into each seed temporary storage device. Each seed temporary storage device is aerated, the liquid strains flow into the spraying device and are ejected through the plurality of sprayers, and the plurality of cultivation bottles are subjected with to inoculation. By the technical scheme, the inoculation on the cultivation bottles has the advantages of uniformity and reliability, high inoculation efficiency, controlled inoculation amount of the liquid strains and the like.
Description
Technical field
The present invention relates to bioengineering field, particularly a kind of inoculation method of operating.
Background technology
Edible mushroom is one of food that is of high nutritive value, and the edible mushroom artificial cultivation technique has very large development in recent years, and the growing and cultivation technology of edible mushroom also develops to automation, batch production from the manually plantation in past gradually.And in the planting process of edible mushroom, the inoculating process of edible fungus species directly plays vital impact to the growth of edible mushroom.
At present; the bacterial classification of edible mushroom mainly be divided into solid spawn and and liquid spawn; wherein; the relative solid spawn of liquid spawn has short, fast, the advantage such as biological transformation ratio is high, bacterial classification purity height of bacterium of incubation time; and the scale of being more convenient for, continuously production are the trend of mushroom industry batch production Development Technology innovation.When liquid spawn inoculates, usually adopt a spray equipment, spray equipment is provided with a plurality of shower nozzles, and 16 shower nozzles are set usually, in order to improve inoculation efficiency.But when existing like this liquid spawn to spray, because the factor such as shower nozzle is too much, pressure drop is higher causes the liquid-spawn inoculation problem of non-uniform.
Summary of the invention
The object of the invention is to, provide a kind of raising liquid spawn to produce the inhomogeneity method of operating of inoculation, solve above technical problem.
Technical problem solved by the invention can realize by the following technical solutions:
Improve liquid spawn and produce the inhomogeneity method of operating of inoculation, adopt inoculating facility that a plurality of culture bottles are inoculated, described inoculating facility comprise the seed temporary storage, with the spray equipment of described seed temporary storage UNICOM, described spray equipment is provided with a plurality of shower nozzles; When inoculation, a plurality of described culture bottles lay respectively at described shower nozzle below, at least two described seed temporary storages of a described spray equipment UNICOM; Each described shower nozzle below is placed with respectively a described culture bottle;
Also be provided with a seeding tank, described seeding tank is respectively by at least two described seed temporary storages of peristaltic pump UNICOM; The control peristaltic pump is squeezed into the liquid spawn in the seeding tank in each seed temporary storage;
Each described seed temporary storage is inflated, and liquid spawn flows in the described spray equipment, by a plurality of shower nozzles liquid spawn is sprayed, and a plurality of described culture bottles are inoculated.
The present invention, inflates respectively a plurality of seed temporary storages when inoculation by a spray equipment is carried out UNICOM with a plurality of seed temporary storages, and the liquid spawn so that a plurality of seed temporary storage is all gushed out enters spray equipment.After adopting above-mentioned design, shower nozzle ejection pressure is sufficient, and shower nozzle ejection liquid spawn is effective, evenly reliable, has realized the purpose that combined pressure is sprayed.
Adopt in addition peristaltic pump that liquid spawn is squeezed into the flow that each seed temporary storage can realize flowing into by peristaltic pump control the liquid spawn of spray equipment.
Air pressure in the described seeding tank is controlled in the 0.1Mpa scope.
When each described seed temporary storage was inflated, the air pressure in the described seed temporary storage was controlled at the 0.1Mpa scope that is no more than, and realized the combined pressure sprinkling.
When each described seed temporary storage was inflated, it was inner to adopt aerating device by gas tube gas to be filled with the seed temporary storage.
Gas tube preferably is deep into the liquid level below of liquid spawn, so that the gas outlet of gas tube is immersed in the liquid in the liquid spawn.Rather than be arranged in the air of seed temporary storage, so that air pressure directly acts on liquid spawn, liquid spawn flows into spray equipment easily.In addition, gas tube in carrying out gas replenishment process, stirs liquid spawn after being deep into liquid spawn liquid level below, so that the liquid spawn in the seed temporary storage keeps evenly.
Described seed temporary storage connects described spray equipment by tube connector, and described tube connector preferably is deep into the liquid level below of liquid spawn.Tube connector preferably adopts flexible pipe.
The gas that described aerating device is filled with for described seed temporary storage preferably adopts by the Purge gas after purifying.In order to keep the quality of liquid spawn.
The present invention can also adopt the mode of automatic control to control inoculation:
At least two aerating devices, at least two peristaltic pump difference link control modules;
In the seed temporary storage, can be provided with pressure sensor, the pressure sensor link control module, control module is by the air pressure in control aerating device and then the control seed temporary storage;
Described control module is by the flow of control peristaltic pump and then control liquid spawn.
In order to raise the efficiency, can adopt at least two described spray equipments to inoculate at culture bottle a plurality of respectively, each described spray equipment all connects at least two described seed temporary storages.
When culture bottle was inoculated, each described spray equipment preferably was provided with eight described shower nozzles, and a culture bottle is all placed in eight described shower nozzle belows, and eight culture bottles are inoculated.
Also can adopt two described spray equipments, each described spray equipment is equipped with 16 described shower nozzles, and a culture bottle is all placed in 16 shower nozzle belows, and 16 culture bottles are inoculated.In order to greatly improve under the prerequisite of inoculation efficiency, evenly reliable to the inoculation of each culture bottle.
Beneficial effect: owing to adopt technique scheme, the present invention has advantages such as the inoculation of culture bottle are even reliably, inoculation efficiency inoculum concentration high, liquid spawn is controlled.
Description of drawings
Fig. 1 is integrated connection schematic diagram of the present invention;
Fig. 2 is a kind of inflation schematic diagram of the present invention.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, further set forth the present invention below in conjunction with concrete diagram.
With reference to Fig. 1, improve liquid spawn and produce the inhomogeneity method of operating of inoculation, adopt inoculating facility that a plurality of culture bottles 1 are inoculated, inoculating facility comprise seed temporary storage 21, with the spray equipment 22 of seed temporary storage 21 UNICOMs, spray equipment 22 is provided with a plurality of shower nozzles 23.When inoculation, a plurality of culture bottles 1 lay respectively at spray equipment 22 belows, at least two seed temporary storages 21 of spray equipment 22 UNICOMs.A plurality of shower nozzles 23 belows are placed with the culture bottle 1 of respective amount.Also be provided with a seeding tank 4, seeding tank 4 is respectively by at least two seed temporary storages of peristaltic pump 41 UNICOMs.Control peristaltic pump 41 is squeezed into the liquid spawn in the seeding tank 4 in each seed temporary storage.Each seed temporary storage 21 is inflated, and liquid spawn flows into spray equipment 22,, with the liquid spawn ejection a plurality of culture bottles 1 is inoculated by a plurality of shower nozzles 23.The present invention, inflates respectively a plurality of seed temporary storages 21 when inoculation by a spray equipment 22 is carried out UNICOM with a plurality of seed temporary storages 21, and the liquid spawn so that a plurality of seed temporary storage 21 is all gushed out enters spray equipment 22.After adopting above-mentioned design, shower nozzle 23 ejection pressure are sufficient, and shower nozzle 23 ejection liquid spawns are effective, evenly reliable, realized the purpose that combined pressure is sprayed.Adopt in addition peristaltic pump 41 that liquid spawn is squeezed into the flow that each seed temporary storage 21 can realize flowing into by peristaltic pump 41 controls the liquid spawn of spray equipment 22.Air pressure in the seeding tank 4 is controlled in the 0.1Mpa scope.
With reference to Fig. 2, when each seed temporary storage 21 was inflated, the air pressure in the seed temporary storage 21 was controlled at the 0.1Mpa scope that is no more than, and realized the combined pressure sprinkling.When each seed temporary storage 21 is inflated, can adopt aerating device 31 by gas tube 32 gas to be filled with seed temporary storage 21 inside.Gas tube 32 preferably is deep into the liquid level below of liquid spawn, so that the gas outlet of gas tube 32 is immersed in the liquid in the liquid spawn.Seed temporary storage 21 connects spray equipment 22 by tube connector, and tube connector preferably adopts flexible pipe.Tube connector preferably is deep into the liquid level below of liquid spawn.Gas tube 32 and tube connector are deep in the liquid spawn respectively, rather than are arranged in the air of seed temporary storage 21, so that air pressure directly acts on liquid spawn, liquid spawn flows into spray equipment 22 easily.In addition, gas tube 32 in carrying out gas replenishment process, stirs liquid spawn after being deep into liquid spawn liquid level below, so that the liquid spawn in the seed temporary storage 21 keeps evenly.The gas that aerating device 31 is filled with for seed temporary storage 21 preferably adopts by the Purge gas after purifying.In order to keep the quality of liquid spawn.
The present invention can also adopt the mode of automatic control to control inoculation: at least two aerating devices 31, at least two peristaltic pump 41 difference link control modules.Can be provided with pressure sensor in that seed temporary storage 21 is interior, the pressure sensor link control module, control module is by the air pressure in control aerating device 31 and then the control seed temporary storage 21.Because peristaltic pump 41 is pumps of a kind of metered flow, control module is by the flow of control peristaltic pump 41 and then control liquid spawn.
In order to raise the efficiency, can adopt at least two spray equipments 22 to inoculate at culture bottle 1 a plurality of respectively, each spray equipment 22 all connects at least two seed temporary storages 21.
When culture bottle 1 was inoculated, each spray equipment 22 preferably was provided with 23, eight shower nozzle 23 belows of eight shower nozzles and all places a culture bottle 1, and eight culture bottles 1 are inoculated.
Embodiment 1:
Equipment connection: adopt two spray equipments 22, each spray equipment 22 is equipped with 23, ten six shower nozzle 23 belows of eight shower nozzles and places respectively a culture bottle 1; Each spray equipment 22 connects two seed temporary storages 21 by flexible pipe respectively, and tube connector is deep into the liquid level below of the liquid spawn of seed temporary storage 21;
Two seed temporary storages 21 connect respectively same seeding tank 4 by peristaltic pump 41 independently respectively;
Aerating device 31 connects gas tube 32, and gas tube 32 is deep into the liquid level below of the liquid spawn of seed temporary storage 21.
Inoculation: control module control aerating device 31 is filled with seed temporary storage 21 inside by gas tube 32 with gas, and the air pressure in the seed temporary storage 21 is controlled at the 0.1Mpa scope that is no more than; The liquid spawn seed temporary storage of gushing out arrives spray equipment 22, and shower nozzle 23 ejections of liquid spawn from the spray equipment 22 fall into the culture bottle 1 of shower nozzle 23 belows, by peristaltic pump 41 metering liquid bacterial classification amounts, after reaching inoculum concentration, control module control peristaltic pump 41 stops, and finishes inoculation.
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and the specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (10)
1. improve liquid spawn and produce the inhomogeneity method of operating of inoculation, adopt inoculating facility that a plurality of culture bottles are inoculated, it is characterized in that, described inoculating facility comprise the seed temporary storage, with the spray equipment of described seed temporary storage UNICOM, described spray equipment is provided with a plurality of shower nozzles; When inoculation, a plurality of described culture bottles lay respectively at described shower nozzle below, at least two described seed temporary storages of a described spray equipment UNICOM; Each described shower nozzle below is placed with respectively a described culture bottle;
Also be provided with a seeding tank, described seeding tank is respectively by at least two described seed temporary storages of peristaltic pump UNICOM; The control peristaltic pump is squeezed into the liquid spawn in the seeding tank in each seed temporary storage;
Each described seed temporary storage is inflated, and liquid spawn flows in the described spray equipment, by a plurality of shower nozzles liquid spawn is sprayed, and a plurality of described culture bottles are inoculated.
2. raising liquid spawn according to claim 1 produces the inhomogeneity method of operating of inoculation, it is characterized in that: the air pressure in the described seeding tank is controlled in the 0.1Mpa scope.
3. raising liquid spawn according to claim 1 produces the inhomogeneity method of operating of inoculation, it is characterized in that: when each described seed temporary storage is inflated, air pressure in the described seed temporary storage is controlled at the 0.1Mpa scope that is no more than, and realizes the combined pressure sprinkling.
4. raising liquid spawn according to claim 3 produces the inhomogeneity method of operating of inoculation, it is characterized in that: when each described seed temporary storage was inflated, it was inner to adopt aerating device by gas tube gas to be filled with the seed temporary storage.
5. raising liquid spawn according to claim 4 produces the inhomogeneity method of operating of inoculation, it is characterized in that: gas tube is deep into the liquid level below of liquid spawn, so that the gas outlet of gas tube is immersed in the liquid in the liquid spawn.
6. raising liquid spawn according to claim 5 produces the inhomogeneity method of operating of inoculation, and it is characterized in that: described seed temporary storage connects described spray equipment by tube connector, and described tube connector is deep into the liquid level below of liquid spawn.
According to claim 1 in 6 the described raising of any one liquid spawn produce the inhomogeneity method of operating of inoculation, it is characterized in that: the gas that described aerating device is filled with to described seed temporary storage adopts by the Purge gas after purifying.
According to claim 1 in 6 the described raising of any one liquid spawn produce the inhomogeneity method of operating of inoculation, it is characterized in that: at least two described aerating devices, at least two described peristaltic pumps are link control modules respectively;
In the seed temporary storage, be provided with pressure sensor, the pressure sensor link control module, control module is by the air pressure in control aerating device and then the control seed temporary storage;
Described control module is by the flow of control peristaltic pump and then control liquid spawn.
According to claim 1 in 6 the described raising of any one liquid spawn produce the inhomogeneity method of operating of inoculation, it is characterized in that: adopt at least two described spray equipments to inoculate at culture bottle a plurality of respectively, each described spray equipment all connects at least two described seed temporary storages.
10. raising liquid spawn according to claim 9 produces the inhomogeneity method of operating of inoculation, it is characterized in that: adopt two described spray equipments, each described spray equipment is equipped with eight described shower nozzles, a culture bottle is all placed in 16 shower nozzle belows, and 16 culture bottles are inoculated.
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| CN2012103163393A CN102960181A (en) | 2012-08-30 | 2012-08-30 | Operation method for improving production inoculation uniformity of liquid strains |
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| CN2012103163393A CN102960181A (en) | 2012-08-30 | 2012-08-30 | Operation method for improving production inoculation uniformity of liquid strains |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145121A (en) * | 2015-10-16 | 2015-12-16 | 中国科学院天津工业生物技术研究所 | Inoculating device for liquid strains for mushroom cultivating |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003009657A (en) * | 2001-06-29 | 2003-01-14 | Nippon Seiki Co Ltd | Apparatus for liquid spawn inoculation |
| JP2004180538A (en) * | 2002-11-29 | 2004-07-02 | Nippon Seiki Co Ltd | Method for inoculating liquid spawn and device therefor |
| CN2764786Y (en) * | 2003-08-21 | 2006-03-15 | 汪志新 | Spraying inoculator for Chinese caterpillar fungus liquid bacterial |
| CN101343612A (en) * | 2007-07-13 | 2009-01-14 | 刘永昶 | Automatic vaccination machine for liquid bacterial |
| CN102550291A (en) * | 2012-01-18 | 2012-07-11 | 山西强盛种业有限公司 | Edible fungus liquid strain inoculation and injection gun |
-
2012
- 2012-08-30 CN CN2012103163393A patent/CN102960181A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003009657A (en) * | 2001-06-29 | 2003-01-14 | Nippon Seiki Co Ltd | Apparatus for liquid spawn inoculation |
| JP2004180538A (en) * | 2002-11-29 | 2004-07-02 | Nippon Seiki Co Ltd | Method for inoculating liquid spawn and device therefor |
| CN2764786Y (en) * | 2003-08-21 | 2006-03-15 | 汪志新 | Spraying inoculator for Chinese caterpillar fungus liquid bacterial |
| CN101343612A (en) * | 2007-07-13 | 2009-01-14 | 刘永昶 | Automatic vaccination machine for liquid bacterial |
| CN102550291A (en) * | 2012-01-18 | 2012-07-11 | 山西强盛种业有限公司 | Edible fungus liquid strain inoculation and injection gun |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145121A (en) * | 2015-10-16 | 2015-12-16 | 中国科学院天津工业生物技术研究所 | Inoculating device for liquid strains for mushroom cultivating |
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Application publication date: 20130313 |