CN102952171A - Method for extracting camellia oleifera seed protein from camellia oleifera seed meal - Google Patents
Method for extracting camellia oleifera seed protein from camellia oleifera seed meal Download PDFInfo
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- CN102952171A CN102952171A CN2012104380250A CN201210438025A CN102952171A CN 102952171 A CN102952171 A CN 102952171A CN 2012104380250 A CN2012104380250 A CN 2012104380250A CN 201210438025 A CN201210438025 A CN 201210438025A CN 102952171 A CN102952171 A CN 102952171A
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- seed meal
- camellia seed
- camellia oleifera
- oleifera seed
- albumen
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Abstract
The present invention provides a method for extracting camellia oleifera seed protein from camellia oleifera seed meal. The method comprises: crushing camellia oleifera seed meal, adopting a compound enzyme to carry out a pretreatment, and then carrying out ultrasonic alkaline extraction, acid precipitation, centrifugation and freeze drying to obtain the crude camellia oleifera seed protein. According to the present invention, the compound enzyme pretreatment is adopted, and then the ultrasonic assisted extraction is adopted so as to shorten an extraction time, reduce energy consumption, and improve camellia oleifera seed protein extraction rate, wherein the camellia oleifera seed protein extraction rate is increased to 68% from 46.8%. In addition, purposes of turning of waste into treasure, low energy consumption and low pollution are achieved.
Description
Technical field
The present invention relates to the tea seed manufacture field, be specifically related to a kind of method of from camellia seed meal, extracting tea seed albumen.
Background technology
Oil tea (Camellia Oleifera) is evergreen dungarunga, belongs to Theaceae, and Camellia is a kind of good traditional oil tree of south China knob.China produces tea big country, and only the cultivated area of camellia oleifera lam just reaches 4,000,000 hm
2, be the country that the oil tea kind is at most in the world, distribution is the widest, tea seed output is the highest, the annual output of producing about 54.98 ten thousand tons of tea seed, 200,000 tons of camellia seed oils, tea seed byproduct-camellia seed meal per year is about 39.71 ten thousand tons.Protein content is about 12%～15% in the camellia seed meal, and its amino acid consists of 17 kinds, and wherein 8 kinds is essential amino acid.Therefore, camellia seed meal albumen has very high economic worth, can be with it as the protein fortifier of producing protein drinks, reconstituted food, bakery product and as the protein raw materials of the leavened prods such as soy sauce.Therefore, the extraction albumen take camellia seed meal as raw material is developed and utilization is very significant.
Summary of the invention
The invention provides a kind of method of from camellia seed meal, extracting tea seed albumen.Realize taking full advantage of of resource, turn waste into wealth, reduce the green economy purpose of polluting.
The technical solution used in the present invention is for achieving the above object:
A kind of method of extracting tea seed albumen from camellia seed meal is characterized in that its extraction process route is: the degreasing camellia seed meal pulverizes → ultrasonic help carry → centrifugal → supernatant liquor → acid sinks → centrifugal → lyophilize → crude protein, specifically may further comprise the steps:
(1) be that 1:24-26 mixes with water with crossing 60 mesh sieves after the pulverizing of degreasing camellia seed meal by solid-liquid ratio, and adjusting pH value is that 5.0～5.5 rear every gram camellia seed meals are to the saccharifying enzyme of the α-amylase that wherein adds 200-250u, 50-100u, stir enzymolysis 4h at 50 ℃, behind the enzymolysis temperature risen to 85 ℃ of enzyme 6min that go out, filtration obtains filter residue and enzymolysis filtrate, in filter residue, add camellia seed meal grain weight amount 10-20 water doubly, at 60 ℃, 20kHz supersound extraction 1-1.5h, get ultrasonic filtrate after the filtration;
(2) merging enzymolysis filtrate and ultrasonic filtrate and obtain water extraction liquid, regulate pH to 10 and in temperature be under 44-46 ℃ the condition, with the ultrasonic lixiviate 60min of 20kHz, is that the whizzer of 3000r/min carries out centrifugation 20min, the collection supernatant liquor with rotating speed;
(3) in above-mentioned gained supernatant liquor, add hydrochloric acid, regulate pH value to 4.5, precipitation, precipitation temperature is 50-60 ℃, sedimentation time is 1.5-2.5 hour;
(4) then centrifugation, throw out distilled water wash 2-3 time, lyophilize namely gets camellia seed meal albumen.
The described method of extracting tea seed albumen from camellia seed meal, it is characterized in that: the described solid-liquid ratio of step (1) is 1:25.
The described method of extracting tea seed albumen from camellia seed meal, it is characterized in that: the precipitation temperature of step (3) is 45 ℃.
The described method of extracting tea seed albumen from camellia seed meal, it is characterized in that: the sedimentation time of step (3) is 2.0h.
Beneficial effect of the present invention:
The present invention adopts the prozyme pre-treatment, adopts ultrasonic helping to carry again, and has shortened extraction time, has reduced energy consumption, has also improved simultaneously the extraction yield of leached tea oil slag albumen, brings up to 68% by original 46.8%, has realized turning waste into wealth less energy-consumption, oligosaprobic purpose.
Embodiment
The degreasing camellia seed meal is pulverized rear 60 mesh sieves of crossing, taking by weighing 30g is that 1:25 mixes with water by solid-liquid ratio, and adjusting pH value is the 5.2 backward α-amylase of 6500u, the saccharifying enzyme of 2100u of wherein adding, stir enzymolysis 4h at 50 ℃, behind the enzymolysis temperature risen to 85 ℃ of enzyme 6min that go out, filter and obtain filter residue and enzymolysis filtrate, add the water of 20 times of camellia seed meal grain weight amounts in the filter residue, at 60 ℃, 20kHz supersound extraction 1.5h, get ultrasonic filtrate after the filtration;
(2) merging enzymolysis filtrate and ultrasonic filtrate and obtain water extraction liquid, regulate pH to 10 and in temperature be under 44-46 ℃ the condition, with the ultrasonic lixiviate 60min of 20kHz, is that the whizzer of 3000r/min carries out centrifugation 20min, the collection supernatant liquor with rotating speed;
(3) in above-mentioned gained supernatant liquor, add hydrochloric acid, regulate pH value to 4.5, precipitation, precipitation temperature is 55 ℃, sedimentation time is 2 hours;
(4) then centrifugation, throw out distilled water wash 2-3 time, lyophilize namely gets the camellia seed meal crude protein, and the extraction yield that calculates the camellia seed meal crude protein is 67.8%.Pulverizing obtains tea seed crude protein powder.
Testing data
The tea seed crude protein powder organoleptic feature index that present embodiment obtains sees Table 1.Detect tea seed protein powder quality index and see Table 2.
Table 1 tea seed protein powder organoleptic feature index
| Project | The tea seed protein powder |
| Form | Powdery is without caking phenomenon |
| Color and luster | White is to light yellow |
| Smell | Has the intrinsic smell of tea seed protein powder, free from extraneous odour |
| Impurity | Without macroscopic tramp material |
Table 2 tea seed protein powder quality examination result
| Nitrogen soluble index (NSI)/% | ≥ 70 | 72 |
| Moisture/%≤ | 10.0 | 5.5 |
| Crude fat (butt)/%≤ | 2.5 | 1.5 |
| Robust fibre (butt)/%≤ | 5.0 | 2.4 |
| Ash content (butt)/%≤ | 6.5 | 3.5 |
| Ash insoluble in HCl/%≤ | 0.03 | 0.01 |
| Granularity (100 mesh sieve percent of pass) 〉= | 95% | 98% |
| Gossypol content mg/kg≤ | 200 | 120 |
Claims (4)
1. method of from camellia seed meal, extracting tea seed albumen, it is characterized in that, its extraction process route is: the degreasing camellia seed meal pulverizes → ultrasonic help carry → centrifugal → supernatant liquor → acid sinks → centrifugal → lyophilize → crude protein, specifically may further comprise the steps:
(1) be that 1:24-26 mixes with water with crossing 60 mesh sieves after the pulverizing of degreasing camellia seed meal by solid-liquid ratio, and adjusting pH value is that 5.0～5.5 rear every gram camellia seed meals are to the saccharifying enzyme of the α-amylase that wherein adds 200-250u, 50-100u, stir enzymolysis 4h at 50 ℃, behind the enzymolysis temperature risen to 85 ℃ of enzyme 6min that go out, filtration obtains filter residue and enzymolysis filtrate, in filter residue, add camellia seed meal grain weight amount 10-20 water doubly, at 60 ℃, 20kHz supersound extraction 1-1.5h, get ultrasonic filtrate after the filtration;
(2) merging enzymolysis filtrate and ultrasonic filtrate and obtain water extraction liquid, regulate pH to 9-10 and in temperature be under 44-46 ℃ the condition, with the ultrasonic lixiviate 60min of 20kHz, is that the whizzer of 3000r/min carries out centrifugation 20min, the collection supernatant liquor with rotating speed;
(3) in above-mentioned gained supernatant liquor, add hydrochloric acid, regulate pH value to 4.5, precipitation, precipitation temperature is 50-60 ℃, sedimentation time is 1.5-2.5 hour;
(4) then centrifugation, throw out distilled water wash 2-3 time, lyophilize namely gets camellia seed meal albumen.
2. the method for extracting tea seed albumen from camellia seed meal according to claim 1, it is characterized in that: the described solid-liquid ratio of step (1) is 1:25.
3. the method for extracting tea seed albumen from camellia seed meal according to claim 1, it is characterized in that: the precipitation temperature of step (3) is 45 ℃.
4. the method for extracting tea seed albumen from camellia seed meal according to claim 1, it is characterized in that: the sedimentation time of step (3) is 2.0h.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392905A (en) * | 2013-08-13 | 2013-11-20 | 新疆农业大学 | Method for preparing albumen powder from hot-pressing vegetable fat cake |
| CN103627765A (en) * | 2013-11-29 | 2014-03-12 | 衢州刘家香食品有限公司 | Preparation method for camellia seed polypeptide |
| CN103626833A (en) * | 2013-11-29 | 2014-03-12 | 衢州刘家香食品有限公司 | Method for extracting camellia seed meal protein employing ultrasonic assisted enzymolysis |
| CN103694310A (en) * | 2013-12-27 | 2014-04-02 | 南昌大学 | Separation method for high-quality cashew proteins |
| CN109369774A (en) * | 2018-11-23 | 2019-02-22 | 盐城工学院 | The extracting method of protein in a kind of acer truncatum seed dregs of rice |
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| CN101828628A (en) * | 2010-04-09 | 2010-09-15 | 江苏省农业科学院 | Biological treatment method for effectively extracting rapeseed protein |
| CN102432664A (en) * | 2011-12-29 | 2012-05-02 | 陕西科技大学 | Method for extracting theasaponin from tea seed pulp |
| CN102690320A (en) * | 2012-05-24 | 2012-09-26 | 管天球 | Process for extracting tea cake protein and tea cake tea saponin in one time |
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- 2012-11-06 CN CN2012104380250A patent/CN102952171A/en active Pending
Patent Citations (3)
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| CN101828628A (en) * | 2010-04-09 | 2010-09-15 | 江苏省农业科学院 | Biological treatment method for effectively extracting rapeseed protein |
| CN102432664A (en) * | 2011-12-29 | 2012-05-02 | 陕西科技大学 | Method for extracting theasaponin from tea seed pulp |
| CN102690320A (en) * | 2012-05-24 | 2012-09-26 | 管天球 | Process for extracting tea cake protein and tea cake tea saponin in one time |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392905A (en) * | 2013-08-13 | 2013-11-20 | 新疆农业大学 | Method for preparing albumen powder from hot-pressing vegetable fat cake |
| CN103392905B (en) * | 2013-08-13 | 2014-11-26 | 新疆农业大学 | Method for preparing albumen powder from hot-pressing vegetable fat cake |
| CN103627765A (en) * | 2013-11-29 | 2014-03-12 | 衢州刘家香食品有限公司 | Preparation method for camellia seed polypeptide |
| CN103626833A (en) * | 2013-11-29 | 2014-03-12 | 衢州刘家香食品有限公司 | Method for extracting camellia seed meal protein employing ultrasonic assisted enzymolysis |
| CN103626833B (en) * | 2013-11-29 | 2016-01-13 | 衢州刘家香食品有限公司 | Ultrasonic wave added enzymolysis and extraction camellia seed meal protein process |
| CN103627765B (en) * | 2013-11-29 | 2016-01-13 | 衢州刘家香食品有限公司 | A kind of preparation method of tea seed polypeptide |
| CN103694310A (en) * | 2013-12-27 | 2014-04-02 | 南昌大学 | Separation method for high-quality cashew proteins |
| CN109369774A (en) * | 2018-11-23 | 2019-02-22 | 盐城工学院 | The extracting method of protein in a kind of acer truncatum seed dregs of rice |
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Application publication date: 20130306 |