CN102943032A - Biochip reading instrument and typing method therefor - Google Patents
Biochip reading instrument and typing method therefor Download PDFInfo
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- CN102943032A CN102943032A CN2012102899249A CN201210289924A CN102943032A CN 102943032 A CN102943032 A CN 102943032A CN 2012102899249 A CN2012102899249 A CN 2012102899249A CN 201210289924 A CN201210289924 A CN 201210289924A CN 102943032 A CN102943032 A CN 102943032A
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Abstract
The invention discloses a biochip reading instrument and a typing method therefor. The biochip reading instrument comprises a housing bottom cover, a housing top cover and a reversible surface cover. The housing bottom cover or the housing top cover is provided with a power interface and a data line interface connected to a computer. The biochip reading instrument is characterized in that a mainboard is fixed in a space composed of the housing bottom cover and the housing top cover; high-precision mechanical mobile platforms, a stepping motor, image sensors, a digital camera module, a light source and an adapter plate are fixed on the mainboard; and one of the high-precision mechanical mobile platforms is provided with a tray matching with the reversible surface cover along one axial direction and the other one of the high-precision mechanical mobile platforms is provided with the corresponding image sensor and the digital camera module along the other axial direction. The biochip reading instrument has the advantages of high detection precision, high flux, high speed and low cost.
Description
Technical field
The invention belongs to molecular biological analysis device field, especially relate to a kind of biological chip reading apparatus and classifying method.
Background technology
Biochip (biochip or bioarray) is according to special interactional principle between biomolecules, with the biochemical analysis process integration in chip surface, thereby realize high-throughput rapid detection to DNA, RNA, polypeptide, protein and other biological composition.The biochip concept of narrow sense refers to by different methods biomolecules (oligonucleotide, cDNA, genomic DNA, polypeptide, antibody, antigen etc.) is bonded to the biomolecules dot matrix that forms on the solid phase mediators such as silicon chip, sheet glass (pearl), plastic sheet (pearl), gel, nylon membrane.
Human papillomavirus (HPV) is the pathogenic agent that causes cervical cancer, carries out the detection of HPV somatotype and can well prevent cervical cancer.The reagent that provides at present the HPV somatotype to detect is divided into two large classes, and a kind of is to determine whether to have infected high-risk HPV; Another kind is to determine specifically to have infected which kind of genotypic HPV, has namely realized concrete somatotype.
In existing equipment to the HPV gene type, mostly comparatively expensive, and often can not realize high throughput testing, the patient uses these equipment Inspections, tends to spend high testing cost, is unfavorable for penetration and promotion.
Biochip is exactly the bioinformation microarray, comprise gene chip, protein chip, cell chip and organization chip etc., the biochip that the present invention detects refers in particular to uses microplate apparatus (patent No. ZL200820094742.5) to carry, TMB (3,3', 5,5' – tetramethyl benzidine) biochip of colour developing.
Summary of the invention
One of main purpose of the present invention is to provide a kind of accuracy of detection high, high-throughput, fast, and the comparatively cheap biological chip reading apparatus of cost, solve the defective that prior art exists.
For realizing described purpose, this biological chip reading apparatus comprises the shell bottom, closure head, turning cover, shell bottom or closure head are provided with power interface and can connect the data line interface of computer, are fixed with mainboard in the space that described shell bottom and closure head form, and described mainboard is fixed with the high-accuracy mechanical mobile platform, stepper-motor, image sensor and digital vedio recording module, light source and pinboard; Described high-accuracy mechanical mobile platform one axially is provided with the pallet that is complementary with turning cover; Another axially is provided with image sensor and digital vedio recording module.
Described biochip can be gene chip, protein chip, cell chip and organization chip.
Further improve: described high-accuracy mechanical mobile platform comprises X-axis mobile platform and y-axis shift moving platform, and each mobile platform is provided with the sensor that matches.
Further improve: described X-axis mobile platform is provided with image sensor and digital vedio recording module; Described y-axis shift moving platform is provided with the pallet that is complementary with turning cover; Perhaps described y-axis shift moving platform is provided with image sensor and digital vedio recording module; Described X-axis mobile platform is provided with the pallet that is complementary with turning cover.
Further improve: described image sensor is ccd image sensor, and described light source is led light source.
Another object of the present invention is to provide a kind of method of using biological chip reading apparatus to carry out gene type.
Described method comprises step:
A) in the hybridization plate that is complementary with pallet, place biochip to be checked, described hybridization plate is positioned in the pallet;
B) start biological chip reading apparatus, and described reading apparatus is implemented to make zero setting;
C) drive mobile platform by the computer that is connected with biochip and move, carry out in real time signals collecting;
D) analyze the data that collect, determine genotype.
Further: described biochip is the HPV typing gene chip, and concrete steps are:
A) in the hybridization plate that is complementary with pallet, place biochip to be checked, described hybridization plate is positioned in the pallet;
B) start biological chip reading apparatus, and described reading apparatus is implemented to make zero setting;
C) move by driving mobile platform with the HPV typing gene chip to the computer that is connected, carry out in real time signals collecting;
D) analyze the data that collect, determine the HPV genotype.
Biological chip reading apparatus of the present invention integrates CCD, led light source, high-accuracy mechanical mobile platform, formed a chip scanning platform, analyzing biochips software is installed on the computer and is connected with the USB of reading apparatus by computer reading apparatus is carried out scan control; Reading apparatus carries out optical scanning to biochip, CCD is transformed into electric charge with the optical signal of image to be scanned, then convert the electrical signal to numerary signal by the analog to digital converter chip, reflect that the optical signal of biochip image changes the binary digit electronic signal that computer can be accepted into this moment, deliver to computer by USB interface at last and obtain as a result figure and storing of our macroscopic chip scanning, analyzing biochips software carries out analyzing and processing to the chip scanning image of above-mentioned acquisition again, and finally obtains HPV DNA detection and somatotype result.Biological chip reading apparatus accuracy of detection of the present invention is high, high-throughput, and fast, and cost is comparatively cheap.
Description of drawings
Fig. 1 is biological chip reading apparatus surface structure schematic diagram of the present invention;
Fig. 2 is biological chip reading apparatus mainboard structure schematic diagram of the present invention;
Fig. 3 is biochip circuit control principle drawing of the present invention;
Fig. 4 is typing gene chip reading apparatus operational flowchart of the present invention.
Among the figure:
1, front panel; 2, heelpiece; 3, power switch; 4, pallet; 5, loam cake; 6, X-axis mobile platform; 7, y-axis shift moving platform; 8, stepper-motor; 9, digital vedio recording module; 10, pinboard; 61, X-axis sensor; 71, Y-axis sensor; 81, stepper motor driver plate
Embodiment
Be a kind of HPV typing gene chip reading apparatus as shown in Figures 1 and 2; Comprise shell heelpiece 2, shell front panel 1, turning loam cake 5, shell bottom or closure head are provided with power interface 3 and can connect the data line interface of computer, be fixed with mainboard in the space that described shell heelpiece 2 and shell front panel 1 form, described mainboard is fixed with the high-accuracy mechanical mobile platform, stepper-motor 8, ccd image sensor and digital vedio recording module 9, led light source and pinboard; Described high-accuracy mechanical mobile platform one axially is provided with the pallet 4 that is complementary with turning cover; Another axially is provided with image sensor and digital vedio recording module.The high-accuracy mechanical mobile platform comprises X-axis mobile platform 6 and y-axis shift moving platform 7, and each mobile platform is provided with the sensor 61,71 that matches.
The X-axis mobile platform is provided with image sensor and digital vedio recording module; Described y-axis shift moving platform is provided with the pallet that is complementary with turning cover.According to different designs, also can be provided with image sensor and digital vedio recording module at the y-axis shift moving platform; Described X-axis mobile platform is provided with the pallet that is complementary with turning cover.
Wherein:
Pallet: carry hybridization plate to be detected mobile at the y-axis shift moving platform.
Power switch: be responsible for closing or open other assembly power supplys.
The X-axis sensor: optoelectronic switch, the digital vedio recording module that is used for controlling the X-axis carrying is finished flyback action.
The Y-axis sensor: optoelectronic switch, the pallet that is used for controlling the Y-axis carrying is finished flyback action.
Pinboard 10: the physical interface that the signal transmission of PC and instrument is provided.
Stepper motor driver plate 81: accept the actuating signal that the PC end passes over, actuating signal is made a response control step direction of motor rotation and number of revolutions.
Stepper-motor: move at corresponding platform by the digital vedio recording module on the pallet on the belt drives y-axis shift moving platform and the driving X-axis mobile platform.
The X-axis mobile platform: the X-direction horizontal sliding bar, carrying digital vedio recording module is so that the digital vedio recording module can be finished the X-direction horizontal slip under the driving of stepper-motor.
The y-axis shift moving platform: the Y direction horizontal sliding bar, weight tray is so that the hybridization plate in pallet and the pallet can be finished the Y direction horizontal slip under the driving of stepper-motor.
Digital vedio recording module: be used for each hybridization gene chip in hole in the hybridization plate is carried out IMAQ, and data communication device is crossed the USB line be sent to the PC end.
Figure 3 shows that HPV typing gene chip circuit control principle drawing of the present invention.Biochip moves on the pallet through putting into the instrument y-axis shift behind the chemical colour reaction in porous plate, biochip is transported to instrument internal by pallet through Y direction, the PC end sends the acquired signal instruction, the X-axis mobile camera cooperates the moving pallet of y-axis shift that any chip in the porous plate is carried out controlled IMAQ, the graphic information that collects is sent to the PC end by the USB line, and analysis software is hybridized contained bioinformation in the unknown sample that detects in some position and analysis hybridization point colored intensity and then judgement biochip by contrast pixel difference automatic decision.
Wherein, system power supply partly is converted to the stepper motor driver power supply for the 220V electric main through power supply adaptor, the direct current energy of 16V is provided to the stepper motor driver plate, the stepper motor driver plate is with the rotation of this direct-current drive stepper-motor, and produces the 5V direct current for the circuit working on the plate again.
Wherein, system shell is partly selected the ABS material design, comprises a upper turn-over lid, and system tray can pass in and out Hatch Opening through Y direction, and upper turn-over lid is responsible for opening or closing Hatch Opening.
Wherein, system's stepper-motor drive part is accepted PC sends stepper-motor by usb communication line and control signal alignment stepper motor driver plate action command, the stepper motor driver plate rotates the step number of regulation according to order control X-axis and y-axis stepper motor, inform PC by control signal wire, usb communication line again after finishing.During electrification reset, stepper motor driver plate control X-axis and y-axis stepper motor rotate, according to the variation of correlation slot optical coupling sensor signal, and the zero position of Automatic-searching X-axis and Y direction.
Wherein, system's digital vedio recording module section is connected with PC by the usb communication line, take pictures, and will take photo pass PC back.The digital vedio recording module also transmits PC by the usb communication line stepper-motor drives order and passes to the stepper motor driver plate by control signal wire, and the stepper motor driver plate is finished the signal anti-pass of driving to PC.
The concrete operation step brief overview that the described HPV typing gene chip of use the present embodiment reading apparatus carries out the HPV gene type is as follows:
Start is made zero:The hybridization plate be placed in 4 → open 3 → each assembly energising → PC end assign flyback action signal → actuating signal through 10 be delivered to 81 → 8 under 61 and 71 and 81 co-controllings adjusting 4 and 9 be returned to zero point;
Signals collecting:PC end assign for the signals collecting order → acquisition of particular bore position through 10 be sent to 81 → 81 controls 8 regulate 4 and 9 the relative position on 6 and 7 → when 4 and 9 arrive above the telltale hole to be collected position → the 9 image → picture signals that gather hybridization hybrid chip in 4 are sent to PC through 10 and hold → finish to gather an action.
To collecting signal, further analyze by the software that runs on the control computer.
When the described HPV typing gene chip of concrete use the present embodiment reading apparatus carries out the HPV gene type, can operate by step shown in Figure 4.Specifically comprise:
Preparation of samples
Be ready to be equipped with the microwell plate through the HPV typing gene chip of hybridization colour developing, to be scanned in the microwell plate
The HPV typing gene chip must be guaranteed drying
Chip scanning
Open HPV typing gene chip reading apparatus power supply and HPV typing gene chip analysis software, the pallet that ready microwell plate to be scanned is put into instrument scans, and scan image is kept in the software default folder.
Interpretation of result
Transfer the chip scanning image that " chip scanning " step has been preserved from software, software will carry out interpretation of result to image automatically.As run into can't automatic analysis image, can utilize the Software tool hurdle to carry out manual analysis, draw at last HPV DNA detection and the somatotype result of each sample, and preserve.
Result's output
After the chip image analysis was preserved, the clinical related information input that each sample is corresponding was also preserved.Detected result and relevant clinical information can generate survey report and printout.Assay can be inquired about easily by setting relevant querying condition.Query Result can be derived and be generated CSV format file (available Excel software is opened).
The described HPV typing gene chip of the present embodiment reading apparatus integrates CCD, led light source, high-accuracy mechanical mobile platform, formed a chip scanning platform, HPV typing gene chip analysis software is installed on the computer and is connected with the USB of reading apparatus by computer reading apparatus is carried out scan control; Reading apparatus carries out optical scanning to the HPV typing gene chip, CCD is transformed into electric charge with the optical signal of image to be scanned, then convert the electrical signal to numerary signal by the analog to digital converter chip, reflect that the optical signal of HPV typing gene chip image changes the binary digit electronic signal that computer can be accepted into this moment, deliver to computer by USB interface at last and obtain as a result figure and storing of our macroscopic chip scanning, HPV typing gene chip analysis software carries out analyzing and processing to the chip scanning image of above-mentioned acquisition again, and finally obtains HPV DNA detection and somatotype result.HPV typing gene chip reading apparatus accuracy of detection of the present invention is high, high-throughput, and fast, and cost is comparatively cheap.
Claims (9)
1. biological chip reading apparatus, comprise the shell bottom, closure head, turning cover, shell bottom or closure head are provided with power interface and can connect the data line interface of computer, it is characterized in that: be fixed with mainboard in the space that described shell bottom and closure head form, described mainboard is fixed with the high-accuracy mechanical mobile platform, stepper-motor, image sensor and digital vedio recording module, light source and pinboard; Described high-accuracy mechanical mobile platform one axially is provided with the pallet that is complementary with turning cover; Another axially is provided with image sensor and digital vedio recording module.
2. biological chip reading apparatus as claimed in claim 1, it is characterized in that: described high-accuracy mechanical mobile platform comprises X-axis mobile platform and y-axis shift moving platform, and each mobile platform is provided with the sensor that matches.
3. biological chip reading apparatus as claimed in claim 2, it is characterized in that: described X-axis mobile platform is provided with image sensor and digital vedio recording module; Described y-axis shift moving platform is provided with the pallet that is complementary with turning cover.
4. biological chip reading apparatus as claimed in claim 2, it is characterized in that: described y-axis shift moving platform is provided with image sensor and digital vedio recording module; Described X-axis mobile platform is provided with the pallet that is complementary with turning cover.
5. such as claim 3 or 4 described biological chip reading apparatus, it is characterized in that: described image sensor is ccd image sensor, and described light source is led light source.
6. such as each described biological chip reading apparatus of claim 1-5, it is characterized in that: described biochip is gene chip, protein chip, cell chip and organization chip.
7. biological chip reading apparatus as claimed in claim 6, it is characterized in that: described gene chip is the HPV typing gene chip.
8. a right to use requires 6 described biological chip reading apparatus to carry out the method for gene type, comprises step:
A) in the hybridization plate that is complementary with pallet, place biochip to be checked, described hybridization plate is positioned in the pallet;
B) start biological chip reading apparatus, and described reading apparatus is implemented to make zero setting;
C) drive mobile platform by the computer that is connected with biochip and move, carry out in real time signals collecting;
D) analyze the data that collect, determine genotype.
9. biological chip reading apparatus as claimed in claim 8 carries out the method for gene type, it is characterized in that: described biochip is the HPV typing gene chip, and concrete steps are:
A) in the hybridization plate that is complementary with pallet, place biochip to be checked, described hybridization plate is positioned in the pallet;
B) start biological chip reading apparatus, and described reading apparatus is implemented to make zero setting;
C) move by driving mobile platform with the HPV typing gene chip to the computer that is connected, carry out in real time signals collecting;
D) analyze the data that collect, determine the HPV genotype.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210289924.9A CN102943032B (en) | 2012-08-15 | 2012-08-15 | Biochip reading instrument and typing method therefor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210289924.9A CN102943032B (en) | 2012-08-15 | 2012-08-15 | Biochip reading instrument and typing method therefor |
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| Publication Number | Publication Date |
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| CN102943032A true CN102943032A (en) | 2013-02-27 |
| CN102943032B CN102943032B (en) | 2015-06-03 |
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| CN107271370A (en) * | 2017-07-04 | 2017-10-20 | 九江学院 | A kind of laser ultrasonic detection system and its method detected based on material internal defect |
| CN107796816A (en) * | 2017-11-22 | 2018-03-13 | 中国农业大学 | A kind of device for detecting safety of foods and method based on image recognition principle |
| CN108956464A (en) * | 2018-05-25 | 2018-12-07 | 江苏猎阵生物科技有限公司 | A kind of reading apparatus for biochip test |
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| CN107271370A (en) * | 2017-07-04 | 2017-10-20 | 九江学院 | A kind of laser ultrasonic detection system and its method detected based on material internal defect |
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| CN102943032B (en) | 2015-06-03 |
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