CN201107269Y

CN201107269Y - Centrifugal type PCR analyzer

Info

Publication number: CN201107269Y
Application number: CNU2007201728878U
Authority: CN
Inventors: 冯继宏; 张清悦; 曾毅
Original assignee: Beijing University of Technology
Current assignee: Beijing University of Technology
Priority date: 2007-08-31
Filing date: 2007-08-31
Publication date: 2008-08-27
Anticipated expiration: 2017-08-31

Abstract

The utility model relates to a centrifugal-type PCR analyzer, which belongs to the biology and medicine field. The traditional gene density method used for analyzing the primitive target of the polymerase chain reaction (PCR) has the disadvantages that firstly, a fluorescence detecting module is unfixed, thereby the detecting precision is influenced; secondly, the partial mechanical structure scanned through the instrument is complex, the cost is high, and the instrument is unfavorable for miniaturization. By adopting the centrifugal-type PCR analyzer, the target gene is increased through a variable-temperature metal module controlled by utilizing a DSP circuit, and simultaneously, the fluorescence emitted by detected reactant is detected. Through the innovative centrifugal-type design and an optical path fixing method in the centrifugal-type PCR analyzer, the miniaturization of the product is realized, and the detecting error caused by the mechanical vibration is avoided. The centrifugal-type PCR analyzer is characterized in that the analyzer comprises an optics detecting module (1), a centrifugal-type heat cycling module (2), a DSP controlling circuit (3) and a PC computer (4); wherein, the optics detecting module (1) is fixed at the upper part of the centrifugal-type heat cycling module (2), and the optics detecting module (1) and the centrifugal-typed heat cycling module (2) are respectively connected with the DSP controlling circuit (3), and then is transmitted to the computer (4).

Description

Centrifugal type PCR analyzer

Technical field:

The utility model is an instrument biological, that medical domain is used to analyze polymerase chain reaction (PCR) initial target mrna concentration.

Background technology:

Before the utility model is made, also do not have to detect and the Centrifugal Analyzer device of analyzing polymerase chain reaction initial target mrna concentration.Traditional method is the heating/refrigeration thermocirculator that earlier target gene is placed 48 holes or 96 holes of standard, finish sex change, renaturation and extension that PCR requires, make millions of times of target gene amplifications, then product is carried out fluoroscopic examination, judge whether to have certain target gene.This method has following shortcoming: first: the fluoroscopic examination module is unfixing, the motion of driven by motor fibre-optical probe, and when scanning, the vibrations of optical fiber can influence the stable of light path, thus influence detects degree of accuracy; Second: instrument sweep test complicated in mechanical structure, cost height, quality are big.Scanning head scans on X and Y direction under the drive of motor, needs two motor collaborative works, is unfavorable for miniaturization.

The utility model content

The purpose of this utility model provides a kind of centrifugal type PCR analyzer that can finish the target gene detection.Centrifugal design and path fixing method by innovation have realized the product miniaturization, the detection error of having avoided mechanical vibration to bring.

The technical solution of the utility model is: when utilizing DSP circuit control alternating temperature metal module that target gene is increased, the fluorescence that tested reactant is sent detects.

A kind of centrifugal type PCR analyzer is characterized in that, comprises the optical detection module, centrifugal thermal cycle module, DSP control circuit and computing machine; The optical detection module is fixed on the top of centrifugal thermal cycle module, and optical detection module, centrifugal thermal cycle module connect the DSP control circuit respectively, are sent to computing machine then;

Described centrifugal thermal cycle module comprises the alternating temperature metallic object, alternating temperature metallic object top is provided with equally distributed jack, inserts the test tube in jack, is stamped the heat lid on the test tube, be attached to the semiconductor heating plate of alternating temperature metallic object inboard, cover in alternating temperature metallic object and heat and all be provided with temperature sensor; Be provided with driving wheel below the alternating temperature metallic object, driving wheel is by the stepper motor of band connection synchronously; Stepper motor, temperature sensor connect the DSP control circuit respectively;

The forward and reverse rotation of stepper motor drives band synchronously, and then drives the driving wheel below the alternating temperature metallic object, thus the clockwise and counterclockwise rotation of control alternating temperature metallic object;

Said optical detection module is provided with excitation source from top to bottom successively, optical filter, lens, with horizontal direction be miter angle two to dichronic mirror, excite object lens, and above-mentioned excitation source, optical filter, lens, with horizontal direction be miter angle two to dichronic mirror, excite object lens all with test tube on same vertical axis; Two on the horizontal axis at dichronic mirror place, be provided with narrow band pass filter successively, receiving objective, pin hole, photomultiplier; Photomultiplier connects the DSP control circuit.

The DSP control circuit comprises the dsp controller (TMS320F2812) that links to each other with PC by interface, by the A/D modular converter of DSP, will convert digital signal to from the output signal of stepper motor, temperature sensor and photomultiplier respectively.The drive signal input stepper motor driver of DSP output, the control motor movement; The temperature control signals of DSP output, the temperature of regulating the semiconductor heating plate; The gain control signal of DSP output, the enlargement factor of regulating photomultiplier.

The utility model has been realized the product miniaturization, the detection error of having avoided mechanical vibration to bring.Simultaneously, the reduction of cost helps the popularization of this advanced detection technique, satisfies the detection demand of community medicine and suburban country and district hygiene department.

Description of drawings

Below in conjunction with accompanying drawing the utility model is done and to be described in further detail:

Fig. 1 is the block diagram of centrifugal type PCR analyzer of the present utility model;

Fig. 2 is the synoptic diagram of centrifugal thermal cycle module;

Fig. 3 is the optical detection module diagram;

Embodiment

With reference to Fig. 1, centrifugal type PCR analyzer of the present utility model comprises the optical detection module, centrifugal thermal cycle module, DSP control circuit and computing machine.Its optical detection module is fixed on the top of centrifugal thermal cycle module, and detected signal is sent into the DSP control circuit and carried out data processing, is sent to Computer Analysis and demonstration then.The structure of centrifugal thermal cycle module and optical detection module such as Fig. 2, shown in Figure 3.

Described optical detection module is provided with excitation source from top to bottom successively, optical filter, lens, with horizontal direction be miter angle two to dichronic mirror, excite object lens, and above-mentioned excitation source, optical filter, lens, with horizontal direction be miter angle two to dichronic mirror, excite object lens all with test tube on same vertical axis.Two on the horizontal axis at dichronic mirror place, be narrow band pass filter successively, receiving objective, pin hole, photomultiplier is gathered the DSP of electric signal.Adopt LED as excitation source, not only satisfy light source requirements but also reduce cost, alleviate product weight, be applicable to portable equipment.The exciting light that excitation source sends becomes the monochromatic light of specific wavelength (532nm) by optical filter, compile for directional light by lens again and be radiated at two on dichronic mirror, again by exciting object lens to converge to test tube, make invisible spectro reactant under the effect of exciting light, send fluorescence, fluorescence is radiated at two on dichronic mirror by exciting object lens, through two transmission and reflections to dichronic mirror, filter the monochromatic light that becomes specific wavelength (570nm) by narrow band pass filter again, converge by receiving objective and pin hole again, become electric signal by the photomultiplier reception and be transferred to DSP.

Centrifugal thermal cycle module comprises the alternating temperature metallic object, the equally distributed jack of above the alternating temperature metallic object a circle inserts the test tube in jack, is stamped the heat lid on the test tube, be attached to the semiconductor heating plate of alternating temperature metallic object inboard, cover in alternating temperature metallic object and heat and all be provided with temperature sensor.The forward and reverse rotation of stepper motor drives band synchronously, and then drives the driving wheel below the alternating temperature metallic object, thus the clockwise and counterclockwise rotation of control alternating temperature metallic object.Wherein, the material of alternating temperature metallic object is an argent, and is outer gold-plated, anti-oxidation; Jack be shaped as Eppendorf reaction tube shape, size is fixing, totally 16 jacks; Before experiment, the test tube that will install measured object is earlier put into jack, starts instrument then.In the operational process, the optical detection module maintains static, and on the alternating temperature metallic object test tube in the jack along with alternating temperature metallic object forward and backward rotation, thereby realize detection one by one to each test tube.

Optical detection module and centrifugal thermal cycle module realize collection and processing to fluorescence by the control of DSP control circuit, and are uploaded to the Computer Analysis demonstration.

In the work, the steering order of computing machine output is converted to control signal through the DSP control circuit on the one hand, and control semiconductor heating plate is worked, and satisfies the temperature requirement of pcr amplification; On the other hand, the control step motor rotates, and realizes the rotation of alternating temperature metallic object, thereby finishes successively all checks of measured object in vitro.Simultaneously, the temperature signal of the temperature sensor output in the centrifugal thermal cycle module and the position signalling of stepper motor output, and the electric signal of optical detection module output, A/D interface conversion by DSP is a digital signal respectively, be transferred to computing machine through handling, and send control signal by computing machine; Simultaneous computer is analyzed data and is shown.

Centrifugal type PCR analyzer of the present utility model, when utilizing centrifugal thermal cycle module that target gene is increased, adopted fixing optical detection module that fluorescence is detected, realized the product miniaturization, the detection error of having avoided mechanical vibration to bring can make former detection error drop to (10%～30%) by (20%～50%).

Claims

1, a kind of centrifugal type PCR analyzer is characterized in that, comprises optical detection module (I), centrifugal thermal cycle module (II), DSP control circuit (III) and PC (IV); Optical detection module (I) is fixed on the top of centrifugal thermal cycle module (II), and optical detection module (I), centrifugal thermal cycle module (II) connect DSP control circuit (III) respectively, are sent to computing machine (IV) then;

Described centrifugal thermal cycle module (II) comprises alternating temperature metallic object (1), alternating temperature metallic object (1) top is provided with equally distributed jack (3), insert the test tube (5) in jack (3), be stamped heat lid (4) on the test tube (5), be attached to the inboard semiconductor heating plate (6) of alternating temperature metallic object (1), on alternating temperature metallic object (1) He Regai (4), all be provided with temperature sensor (9); Be provided with driving wheel (2) below the alternating temperature metallic object (1), driving wheel (2) is by the stepper motor (7) of band (8) connection synchronously; Stepper motor (7), temperature sensor (9) connect DSP control circuit (III) respectively;

Said optical detection module (I) is provided with excitation source (10) from top to bottom successively, optical filter (11), lens (12), with horizontal direction be miter angle two to dichronic mirror (13), excite object lens (14), and above-mentioned excitation source (10), optical filter (11), lens (12), with the horizontal direction miter angle two to dichronic mirror (13), excite object lens (14) all with test tube (5) on same vertical axis; Two on the horizontal axis at dichronic mirror (13) place, be provided with narrow band pass filter (18) successively, receiving objective (17), pin hole (16), photomultiplier (15); Photomultiplier (15) connects DSP control circuit (III).