CN102910952A - 一种富硒白灵菇液态发酵培养基 - Google Patents
一种富硒白灵菇液态发酵培养基 Download PDFInfo
- Publication number
- CN102910952A CN102910952A CN2012103892020A CN201210389202A CN102910952A CN 102910952 A CN102910952 A CN 102910952A CN 2012103892020 A CN2012103892020 A CN 2012103892020A CN 201210389202 A CN201210389202 A CN 201210389202A CN 102910952 A CN102910952 A CN 102910952A
- Authority
- CN
- China
- Prior art keywords
- selenium
- pleurotus nebrodensis
- percent
- culture medium
- rich
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明公开了一种富硒白灵菇液态发酵培养基,这种培养基由3.2~3.3%葡萄糖;1.5~1.8%酵母膏;0.2~0.25%KH2PO4;0.1~0.12%MgSO4;0.0025~0.003%Na2SeO3和适量的蒸馏水组成。通过富硒白灵菇液态深层培养,菌丝生物量可达1.336g/100ml,富硒率可达7.51%。
Description
技术领域
本发明涉及食用菌培养基,特别涉及的是一种富硒白灵菇液态发酵培养基。
背景技术
目前作为有机硒制品富硒食用菌的研究和开发倍受关注,但是关于白灵菇富硒液态深层发酵培养的报道甚少,且液体发酵诸因素对菌丝富硒生长的影响尚未见报道。以白灵菇为富硒的载体,将亚硒酸钠添加到培养基中,通过富硒白灵菇液态深层培养,富硒率可达7.51%。
发明内容
本发明的目的是提供的一种富硒白灵菇液态发酵培养基,克服了传统固态培养基菌丝生长慢、富硒率低等缺点。
提供的一种富硒白灵菇液态发酵培养基,其原料由以下重量百分比的组分组成:
葡萄糖:3.2~3.3%;
酵母膏:1.5~1.8%;
KH2PO4:0.2~0.25%;
MgSO4:0.1~0.12%;
Na2SeO3:0.0025~0.003%;
蒸馏水:适量;
以上各组分之和为100%,其特征在于:在上述配料中添加有0.0025~0.003%Na2SeO3。
通过富硒白灵菇液态深层培养,菌丝生物量可达1.336g/100ml,富硒率可达7.51%。
具体实施方式
实施例
葡萄糖:32.7g/L;
酵母膏:16.9g/L;
KH2PO4:2.15g/L;
MgSO4:1g/L;
Na2SeO3:30μg/ml;
添加蒸馏水至1000ml。
通过富硒白灵菇液态深层培养,菌丝生物量可达1.336g/100ml,富硒率可达7.51%。
Claims (1)
1.一种富硒白灵菇液态发酵培养基,由以下重量百分比的原料组成:
葡萄糖:3.2~3.3%;酵母膏:1.5~1.8%;KH2PO4:0.2~0.25%;MgSO4:0.1~0.12%:Na2SeO3:0.0025~0.003%;蒸馏水:适量;以上各组分之和为100%,
其特征在于:在上述配料中添加有0.0025~0.003%Na2SeO3。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2012103892020A CN102910952A (zh) | 2012-09-27 | 2012-09-27 | 一种富硒白灵菇液态发酵培养基 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2012103892020A CN102910952A (zh) | 2012-09-27 | 2012-09-27 | 一种富硒白灵菇液态发酵培养基 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN102910952A true CN102910952A (zh) | 2013-02-06 |
Family
ID=47609559
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2012103892020A Pending CN102910952A (zh) | 2012-09-27 | 2012-09-27 | 一种富硒白灵菇液态发酵培养基 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102910952A (zh) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1888048A (zh) * | 2006-07-21 | 2007-01-03 | 江南大学 | 一种以活化硒矿为硒源的富硒食用菌产品的制备方法 |
CN102246659A (zh) * | 2011-04-21 | 2011-11-23 | 金勇� | 含硒丰富的虫草生产方法 |
-
2012
- 2012-09-27 CN CN2012103892020A patent/CN102910952A/zh active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1888048A (zh) * | 2006-07-21 | 2007-01-03 | 江南大学 | 一种以活化硒矿为硒源的富硒食用菌产品的制备方法 |
CN102246659A (zh) * | 2011-04-21 | 2011-11-23 | 金勇� | 含硒丰富的虫草生产方法 |
Non-Patent Citations (1)
Title |
---|
王尚荣: "白灵菇富硒栽培技术研究", 《食用菌学报》, vol. 10, no. 4, 20 December 2003 (2003-12-20), pages 43 - 46 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Huo et al. | Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China | |
Grobbelaar | Inorganic algal nutrition | |
Junying et al. | Factors in mass cultivation of microalgae for biodiesel | |
Wang et al. | A two-stage fed-batch heterotrophic culture of Chlorella protothecoides that combined nitrogen depletion with hyperosmotic stress strategy enhanced lipid yield and productivity | |
Bezerra et al. | Effects of light intensity and dilution rate on the semicontinuous cultivation of Arthrospira (Spirulina) platensis. A kinetic Monod-type approach | |
KR101694711B1 (ko) | 지질 생산성을 증가시키는 미세조류의 배양방법 | |
CN114196547B (zh) | Dcmu在提高微藻混养发酵时叶绿素产量或强光耐受性中的应用 | |
CN105296376A (zh) | 一种提高斜生栅藻生物量和油脂质量的共生菌体系及其应用 | |
CN102746990A (zh) | 由微藻类制造高含量淀粉的方法 | |
CN102187786A (zh) | 野生冬虫夏草菌种无性繁殖方法 | |
CN109652318A (zh) | 一种通过稳定pH快速培养小球藻的培养基及其应用 | |
CN102910952A (zh) | 一种富硒白灵菇液态发酵培养基 | |
CN114196546B (zh) | DCMU在稳定微藻混养生长pH和提高微藻混养生长速度中的应用 | |
CN103387940A (zh) | 鄂尔多斯高原碱湖钝顶螺旋藻培养基、培养及培养基配制 | |
CN102260633A (zh) | 功能性人工窖泥的研究和应用 | |
Yadavalli et al. | Effect of different culture media on cell concentrations of Chlorella pyrenoidosa under photoautotrophic conditions | |
CN103555588A (zh) | 一种高密度、高油脂含量小球藻细胞的培养方法 | |
Zeng et al. | A Novel Bioflocculant Produced by MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae | |
CN107312721B (zh) | 一种双孢蘑菇母种培养基及其应用 | |
CN104403985A (zh) | 富碘发状念珠藻的培养方法 | |
CN107988078A (zh) | 一种利用木糖混合营养培养小球藻的方法 | |
CN102329005B (zh) | 一种水产养殖用微生物制剂的制备方法 | |
Wang et al. | Microbial oil production by Rhodotorula glutinis CICC 31643 using sugar cane molasses | |
CN108179112A (zh) | 蛋白核小球藻联合菌类产氢的方法 | |
Mutanda et al. | Physico-chemical and biotic factors influencing microalgal seed culture propagation for inoculation of a large scale raceway pond |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130206 |