CN102907671B - Preparation method and application of pectin enzymatic hydrolysis product having functions of dispelling effects of alcohol and resisting drunk - Google Patents
Preparation method and application of pectin enzymatic hydrolysis product having functions of dispelling effects of alcohol and resisting drunk Download PDFInfo
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Abstract
The invention discloses a preparation method and application of pectin enzymatic hydrolysis product having functions of dispelling the effects of alcohol and resisting drunk. Constant-temperature enzymatic hydrolysis and de-fatting are performed to polymer high-fat pectin by pectin esterase and pectinase, filtrate is subjected to alcohol precipitation and alcohol leaching after product is filtered to obtain high-purity product, and the high-purity product is subjected to vacuum drying and crushing and screening to obtain pectin enzymatic hydrolysis product having excellent effects of dispelling the effects of alcohol and resisting drunk. Esterification degree and molecular weight of the pectin are reduced by enzymatic hydrolysis and de-fatting of the pectin, the effects of dispelling the effects of alcohol and resisting drunk of product are enhanced by the pectin compared with by the raw materials. Pectin is deeply developed and utilized, additional value of the pectin is convenient to increase, and application range of pectin is broadened. The enzymatic hydrolysis product has high effects of dispelling the effects of alcohol and resisting drunk and can be used as alcohol-effect-dispelling and drunk-resisting components to be applied to the field of medicine and food.
Description
Technical field
The present invention relates to food, relate in particular to a kind of preparation method and application with the polygalacturonase hydrolysis products that relieves the effect of alcohol and resist drunk function.
Background technology
Natural pectin is a kind of acidic polysaccharose material in plant, for subacid white to pale yellow powder, have water-soluble, molecular weight approximately 50,000 ~ 300,000.Its raw material sources are extensive, mainly contain peel of Citrus reticulata Blanco, lemon peel, pomelo peel, Pericarpium Mali pumilae, beet skin etc.Also contain more rich pectin in sunflower plate, mango slag, onion, silkworm excrement and Japanese Premna in addition.
Due to the good gel-forming property of having of pectin and stabilizing protein performance, therefore being used as the foodstuff additive such as jelling agent, thickening material, stablizer, suspension agent, emulsifying agent is widely used in food-processing industry (Bai Ruiling, Zhang Wenxi, cypress. production research and the detection of pectin. Inner Mongol journal of institutes of education (natural science edition), 1993,3:84-86).Biological activity research about natural pectin increases gradually at present, as having, the pectin oligosaccharides promotes bifidus bacillus propagation, antibacterial, prevent diabetes, anticancer isoreactivity, therefore at field widespread use (Zhang Xuejie such as food, medicine, agricultural, fodder industry, makeup, Guo Ke, Su Yanling. the pectin New research progress. the Chinese food journal, 2010,10(1): 167-174.).But natural pectin is saccharan long-chain carbohydrate, poorly water-soluble, mouthfeel is bad, and therefore, the application of polymer pectin aspect biologic activity is restricted.And polymer pectin product added value is low, carry out the research of pectin correlation properties to improve the scientific and technological content of pectin processing, the function of improving pectin is necessary very much.
Modified pectin (MCP) is that a kind of molecular weight that natural pectin obtains is less, gamma value is lower, branchiess pectin polysaccharide by being hydrolyzed, and molecular weight is reduced to below 20,000.With common pectin, compare, its monosaccharide component does not have basic variation, but MCP more is rich in galacturonic acid, rhamnosyl and wood sugar, and the physiological function MCP that common pectin has has, and has the functions such as better Anti-tumor metastasis, reducing blood-fat.Studies have reported that low-esterified pectin has good effect (Wei Xiaoying aspect deleading.The Receptaculum Helianthi low-ester pectin drives plumbous clinical study. Chinese industrial medical journal, 1992,5 (2): 86-87).The current research majority about the modified pectin characteristic concentrates on to provide and is applicable to antitumor, hypoglycemic or lead-expelling function, and relieves the effect of alcohol and resist the research of the polygalacturonase hydrolysis products performance of drunk function to have no report for being applicable to.
The present invention improves the biological function of pectin by controlling hydrolyzed pectin degree and de-degree of esterification, prepare and there is the polygalacturonase hydrolysis products that well relieves the effect of alcohol and resist drunk function by zymolysis technique, improve the added value of pectin, the range of application of developing pectin product, for using pectin product as relieving the effect of alcohol and anti-drunk composition is applied to medicine and field of food provides theoretical direction and technical support.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of preparation method and application with the polygalacturonase hydrolysis products that relieves the effect of alcohol and resist drunk function is provided.
Preparation method with the polygalacturonase hydrolysis products that relieves the effect of alcohol and resist drunk function is: take polymer high ester pectin powder, cross 40 mesh sieves, add pectin powder dry weight 30-50 water doubly, stir, be heated to 70-90 ℃, cooling, adjust pH, to 4-5, adds the polygalacturonase of pectin powder dry weight 0.03%-0.08%, enzyme digestion reaction 5-30min, the Rohapect MPE that adds pectin powder dry weight 0.3%-0.8%, de-ester reaction 20min-70min; Adjust pH to neutral, filter, obtain filtrate, add the edible ethanol of mass percentage concentration 80% precipitated and filter, filter residue carries out centrifuge dehydration after repetitive scrubbing, vacuum-drying recovered alcohol with the alcohol of mass percentage concentration 80% again, pulverize, cross 40 mesh sieves, obtain the polygalacturonase hydrolysis products.
Described polymer high ester pectin is to derive from lemon peel, peel of Citrus reticulata Blanco or Pericarpium Mali pumilae, and its gamma value is greater than 60%, and molecular-weight average is higher than 100,000 dalton.
In described enzyme digestion reaction, the polygalacturonase add-on is the 0.04%-0.06% of pectin powder dry weight, enzyme digestion reaction 10-20min.
In described de-ester reaction, the Rohapect MPE add-on is the 0.4%-0.7% of pectin powder dry weight, and the de-ester reaction time is 30-60min.
Have relieve the effect of alcohol and the polygalacturonase hydrolysis products of anti-drunk function for the preparation of relieving the effect of alcohol and anti-drunk medicine.Have relieve the effect of alcohol and the polygalacturonase hydrolysis products of anti-drunk function for the preparation of food or healthcare products.
Pectin provided by the invention, by enzymolysis and de-ester, reduced gamma value and the molecular weight of pectin, improved relieving the effect of alcohol and anti-drunk effect of product, with raw material, compare, product relieve the effect of alcohol and anti-drunk effect strengthens.The present invention is the deep development utilization to pectin, is conducive to improve the added value of pectin, opens up its range of application.This enzymolysis product has good relieving the effect of alcohol and anti-drunk effect, can be used as and relieves the effect of alcohol and resist drunk composition to be applied to medicine, field of food.
The accompanying drawing explanation
Fig. 1 is the effect comparison diagram that relieves the effect of alcohol of pectin and enzymolysis product thereof;
Fig. 2 is the anti-drunk effect comparison diagram of pectin and enzymolysis product thereof.
Embodiment
Preparation method with the polygalacturonase hydrolysis products that relieves the effect of alcohol and resist drunk function is: take polymer high ester pectin powder, cross 40 mesh sieves, add pectin powder dry weight 30-50 water doubly, stir, be heated to 70-90 ℃, cooling, adjust pH, to 4-5, adds the polygalacturonase of pectin powder dry weight 0.03%-0.08%, enzyme digestion reaction 5-30min, the Rohapect MPE that adds pectin powder dry weight 0.3%-0.8%, de-ester reaction 20min-70min; Adjust pH to neutral, filter, obtain filtrate, add the edible ethanol of mass percentage concentration 80% precipitated and filter, filter residue carries out centrifuge dehydration after repetitive scrubbing, vacuum-drying recovered alcohol with the alcohol of mass percentage concentration 80% again, pulverize, cross 40 mesh sieves, obtain the polygalacturonase hydrolysis products.
Described polymer high ester pectin is to derive from lemon peel, peel of Citrus reticulata Blanco or Pericarpium Mali pumilae, and its gamma value is greater than 60%, and molecular-weight average is higher than 100,000 dalton.Its objective is the molecular weight and the gamma value that guarantee to control product in de-ester and hydrolytic process, this does not relate to or mentions in pectin modified product preparation in the past.
In described enzyme digestion reaction, the polygalacturonase add-on is the 0.04%-0.06% of pectin powder dry weight, enzyme digestion reaction 10-20min.Its objective is that the molecular weight control guaranteed in hydrolysis reaction is between 20,000-30,000, this has no elaboration in pectin modified product preparation in the past.
In described de-ester reaction, the Rohapect MPE add-on is the 0.4%-0.7% of pectin powder dry weight, and the de-ester reaction time is 30-60min.Its objective is that the gamma value guaranteed in the de-ester reaction process is controlled between 20%-40%, this has no report in the de-ester modified product preparation of pectin in the past.
Have relieve the effect of alcohol and the polygalacturonase hydrolysis products of anti-drunk function for the preparation of relieving the effect of alcohol and anti-drunk medicine.Have relieve the effect of alcohol and the polygalacturonase hydrolysis products of anti-drunk function for the preparation of food or healthcare products.
Embodiment 1
Take polymer high ester pectin powder, cross 40 mesh sieves, add the water of 30 times of pectin powder dry weights, stir, be heated to 70 ℃, cooling, adjust pH to 4, add the polygalacturonase of pectin powder dry weight 0.03%, enzyme digestion reaction 5min, add the Rohapect MPE of pectin powder dry weight 0.3%, de-ester reaction 20min; Adjust pH to neutral, filter, obtain filtrate, add the edible ethanol of mass percentage concentration 80% precipitated and filter, filter residue carries out centrifuge dehydration after repetitive scrubbing, vacuum-drying recovered alcohol with the alcohol of mass percentage concentration 80% again, pulverize, cross 40 mesh sieves, obtain polygalacturonase hydrolysis products 7.5g, yield is 75%.The molecular-weight average 68000 of product, methoxy content 68%.This product is carried out to preventing drunkenness and the experimentation on animals of relieving the effect of alcohol, the results are shown in Figure 1 and Fig. 2.
Embodiment 2
Take polymer high ester pectin powder, cross 40 mesh sieves, add the water of 50 times of pectin powder dry weights, stir, be heated to 90 ℃, cooling, adjust pH to 5, add the polygalacturonase of pectin powder dry weight 0.08%, enzyme digestion reaction 30min, add the Rohapect MPE of pectin powder dry weight 0.8%, de-ester reaction 70min; Adjust pH to neutral, filter, obtain filtrate, add the edible ethanol of mass percentage concentration 80% precipitated and filter, filter residue carries out centrifuge dehydration after repetitive scrubbing, vacuum-drying recovered alcohol with the alcohol of mass percentage concentration 80% again, pulverize, cross 40 mesh sieves, obtain polygalacturonase hydrolysis products 9.2g, yield is 92%.The molecular-weight average 9000 of product, methoxy content 12%.This product is carried out to preventing drunkenness and the experimentation on animals of relieving the effect of alcohol, the results are shown in Figure 1 and Fig. 2.
Embodiment 3
Take polymer high ester pectin powder, cross 40 mesh sieves, add the water of 40 times of pectin powder dry weights, stir, be heated to 80 ℃, cooling, adjust pH to 5, add the polygalacturonase of pectin powder dry weight 0.04%, enzyme digestion reaction 10min, add the Rohapect MPE of pectin powder dry weight 0.4%, de-ester reaction 30min; Adjust pH to neutral, filter, obtain filtrate, add the edible ethanol of mass percentage concentration 80% precipitated and filter, filter residue carries out centrifuge dehydration after repetitive scrubbing, vacuum-drying recovered alcohol with the alcohol of mass percentage concentration 80% again, pulverize, cross 40 mesh sieves, obtain polygalacturonase hydrolysis products 9.0g, yield is 90%.The molecular-weight average 26000 of product, methoxy content 35%.This product is carried out to preventing drunkenness and the experimentation on animals of relieving the effect of alcohol, the results are shown in Figure 1 and Fig. 2.
Embodiment 4
Take polymer high ester pectin powder, cross 40 mesh sieves, add the water of 40 times of pectin powder dry weights, stir, be heated to 80 ℃, cooling, adjust pH to 48, add the polygalacturonase of pectin powder dry weight 0.06%, enzyme digestion reaction 20min, add the Rohapect MPE of pectin powder dry weight 0.7%, de-ester reaction 60min; Adjust pH to neutral, filter, obtain filtrate, add the edible ethanol of mass percentage concentration 80% precipitated and filter, filter residue carries out centrifuge dehydration after repetitive scrubbing, vacuum-drying recovered alcohol with the alcohol of mass percentage concentration 80% again, pulverize, cross 40 mesh sieves, obtain polygalacturonase hydrolysis products 9.1g, yield is 91%.The molecular-weight average 22000 of product, methoxy content 23%.This product is carried out to preventing drunkenness and the experimentation on animals of relieving the effect of alcohol, the results are shown in Figure 1 and Fig. 2.
From Fig. 1 and Fig. 2, can find out, the embodiment of the present invention 3 and 4 is controlled gamma values and molecular weight in claimed range the time, it relieves the effect of alcohol and resists drunk effect to be better than the polymer high ester pectin of embodiment 1, low ester low molecule pectin and the raw material pectin of embodiment 2, suitable with extra large Wang Jin cup effect.Can be used as and relieve the effect of alcohol and resist drunk composition to apply in preparing medicine, healthcare products, food.
In the present invention, the method for determining molecular weight is the high performance liquid phase gel chromatography.Concrete analysis condition is: Waters 515 type gel chromatographs, Ultrahydrogel 1000 (7.8*300mm) chromatographic column, moving phase: 0.1M metabisulfite solution, differential refraction detector, flow velocity 0.5ml/min, sample size 50ul, 45 ℃ of column temperatures.Standard specimen is molecular-weight average and is respectively 401300Da, 276500Da, 196300 Da, 66700Da, 21400Da, the dextran of 9890Da (U.S. Sigma company).
Of the present inventionly relieve the effect of alcohol and resist drunk effect assessment to adopt the experiment of pre-preventing drunkenness and sobering-up functions to test two kinds of animal models.Pre-preventing drunkenness experiment adopts the white wine that alcoholic strength is 52 degree to carry out modeling.Half an hour after administration, the white wine of spending according to the dosage gavage mouse 52 of 0.14mL/10g, record the time that the mouse normal reflection disappears, and measure the alcohol concn in Mouse Blood, detects alcohol absorbing state.The sobering-up functions experiment is to give mouse to righting reflex loss (drunk) by 52 degree white wine gavages, gives immediately pectin and positive drug, observes and respectively organizes the drunk time length of mouse.
Claims (4)
1. the preparation method with the polygalacturonase hydrolysis products that relieves the effect of alcohol and resist drunk function, it is characterized in that: take polymer high ester pectin powder, cross 40 mesh sieves, add pectin powder dry weight 30-50 water doubly, stir, be heated to 70-90 ℃, cooling, adjust pH is to 4-5, the polygalacturonase that adds pectin powder dry weight 0.03%-0.08%, enzyme digestion reaction 5-30min, add the Rohapect MPE of pectin powder dry weight 0.3%-0.8%, de-ester reaction 20min-70min; Adjust pH to neutral, filter, obtain filtrate, add the edible ethanol of mass percentage concentration 80% precipitated and filter, filter residue carries out centrifuge dehydration after repetitive scrubbing, vacuum-drying recovered alcohol with the alcohol of mass percentage concentration 80% again, pulverize, cross 40 mesh sieves, obtain the polygalacturonase hydrolysis products; Described polymer high ester pectin is to derive from lemon peel, peel of Citrus reticulata Blanco or Pericarpium Mali pumilae, and its gamma value is greater than 60%, and molecular-weight average is higher than 100,000 dalton.
2. a kind of preparation method with the polygalacturonase hydrolysis products that relieves the effect of alcohol and resist drunk function according to claim 1, it is characterized in that: in described enzyme digestion reaction, the polygalacturonase add-on is the 0.04%-0.06% of pectin powder dry weight, enzyme digestion reaction 10-20min.
3. a kind of preparation method with the polygalacturonase hydrolysis products that relieves the effect of alcohol and resist drunk function according to claim 1, it is characterized in that: in described de-ester reaction, the Rohapect MPE add-on is the 0.4%-0.7% of pectin powder dry weight, the de-ester reaction time is 30-60min.
One kind as claimed in claim 1 having of preparing of method relieve the effect of alcohol and the application of the polygalacturonase hydrolysis products of anti-drunk function, it is characterized in that: for the preparation of relieving the effect of alcohol and anti-drunk medicine.
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| CN103275242B (en) * | 2013-06-18 | 2015-11-11 | 烟台安德利果胶股份有限公司 | A kind of production technique of pectin |
| CN104277946B (en) * | 2013-07-08 | 2016-08-10 | 孟庆梅 | Functional type yellow wine and preparation method thereof |
| CN104419737B (en) * | 2013-08-28 | 2018-02-06 | 浙江果源康品生物科技有限公司 | The enzymolysis preparation of low molecule pectin |
| CN113025674B (en) * | 2021-03-16 | 2022-07-05 | 遵义医科大学 | Preparation method and application of lemon pectin enzymolysis product with anti-tumor activity |
| CN114916666A (en) * | 2022-04-20 | 2022-08-19 | 杭州曼士元食品科技有限公司 | Composition for relieving alcoholism |
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| DE10057976B4 (en) * | 2000-11-22 | 2005-02-03 | Südzucker AG Mannheim/Ochsenfurt | Process for the preparation of pectin hydrolysis products |
| CN101613378B (en) * | 2009-07-17 | 2012-07-04 | 辽宁大学 | Acidic oligosaccharide having weight-reducing and lipid-lowering effects and application thereof |
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