CN102895308A - New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle - Google Patents
New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle Download PDFInfo
- Publication number
- CN102895308A CN102895308A CN2012104503706A CN201210450370A CN102895308A CN 102895308 A CN102895308 A CN 102895308A CN 2012104503706 A CN2012104503706 A CN 2012104503706A CN 201210450370 A CN201210450370 A CN 201210450370A CN 102895308 A CN102895308 A CN 102895308A
- Authority
- CN
- China
- Prior art keywords
- salvianolic acid
- cosmetics
- application
- skin
- whitening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to new use of B Salvianolic acid. The B Salvianolic acid has a strong inhibition effect to tyrosinase, thereby being used for whitening skin, removing freckle, resisting wrinkle, and treating hyperpigmentation diseases, and being applicable to food additives. The inhibition of the B Salvianolic acid to the activity of the tyrosinase is detected, the B Salvianolic acid is very high in the activity of the tyrosinase, and is better in stability, and the B Salvianolic acid can effectively inhibit the generation of the melanin, and can be taken as the natural skin-whitening agent for cosmetics. Meanwhile, the B Salvianolic acid can more preferably promote the skin cell proliferation function, and has the functions of beautifying and resisting wrinkle, thereby being applicable to the cosmetics with the functions of beautifying and resisting wrinkle.
Description
Technical field
The present invention relates to salvianolic acid as the new purposes of whitening and speckle dispelling anti-wrinkle cosmetic additive.
Background technology
Along with expanding economy and growth in the living standard, people more and more pay attention to the natural whitening of skin, " skin is such as snow; solidifying such as fat " always is the skin that the Asians advocates, many people's expectations obtain skin pale, that go window-shopping by the use of Skin whitening care cosmetics, this just is suppress dermal melanin synthetic, thereby the various whitening chemical compounds that reach the skin whitening effect provide wide application prospect, and promotes the production and sales of these class cosmetics.Skin-lightening cosmetic in the past is in the majority mainly with chemical drugs, but because its side effect is large, is banned use of by many countries.The cry that " naturalization of component " reaches " back to nature " has become the trend of cosmetics industry, and naturalization of cosmetic material enjoys attention.China's Chinese Traditional Medicine then take YIN nourishing for oral administration, invigorate blood circulation, the kidney invigorating, spleen invigorating be as main, with external face mask of traditional Chinese medicine and frost, unguentum is in the majority and evident in efficacy, lasting, the advantage such as side effect is little, for pigmentation disease display such as Chinese medicine chloasma good prospect.Therefore, seek efficient, lasting and to the natural whiting speckle dispelling preparation that human body has no side effect, become the trend of present pharmacy and cosmetic field research.Although China's Chinese herbal medicine resource is abundant, the natural plants pharmacy is with a long history, relevant scientific development and applied research relatively lag behind.At present more existing compositions that separate from Chinese herbal medicine are widely used in the whitening product, such as arbutin and Radix Glycyrrhizae extract etc., have become at present the most frequently used whitening composition through repeatedly studying and trying out.Also just because of seen unlimited in the whitening field of conventional medicament, increasing people will seek the target tightening of efficient, lasting whitening composition on natural drug.
Radix Salviae Miltiorrhizae is the Chinese medicine with blood circulation invigorating efficacies, wherein contains the various active compositions such as salvianolic acid, danshensu, TANSHINONES, and cardiovascular and cerebrovascular disease is had significant curative effect.The inventor finds by the research to Radix Salviae Miltiorrhizae active component salvianolic acid, salvianolic acid is except restraint of tyrosinase is active significantly, can suppress significantly melanocyte and form link, to skin-whitening and effective, the safety for the treatment of cutaneous pigmentation disease, be a kind of active skull cap components, good application prospect is arranged.
Simultaneously, the fruit and vegerable fish and shrimp are fresh-keeping also to be the urgent problem that solves that current China agricultural, fishery face, some farming, aquatic products, such as Fructus Litchi, live fish etc., because can not get timely and effectively preservation method processing, bring huge economic loss to China every year.And tryrosinase has participated in the browning reaction of many vegetables and fruits fish and shrimp, is that these fruit and vegerable fish and shrimp are given birth to speckle and lost bright main cause.So by the effect of tryrosinase in the regulation and control fruit and vegerable fish and shrimp, the inhibitor of seeking natural tryrosinase is significant for the fresh-keeping method of fruit and vegerable fish and shrimp.
Summary of the invention
The purpose of this invention is to provide the new purposes of salvianolic acid, salvianolic acid has inhibitory action to tryrosinase, is applied to the beauty face-whitening-nourishing product and improves pigmentation disease.
Salvianolic acid of the present invention derives from the water solubility extract that obtains through extracting purification.Described salvianolic acid is to exist with free acid type form, includes the Radix Salviae Miltiorrhizae total phenolic acids of salviol acid A, salvianolic acid B, salvianolic acid C etc.
By the mensuration to salvianolic acid restraint of tyrosinase activity, find that salvianolic acid shows tyrosinase inhibitory activity.In test cell line, find that salvianolic acid also can reduce the activity of intracellular tyrosine enzyme, can reduce again the biosynthetic level of melanocyte in the cell simultaneously.
Contain a large amount of reproducibility polyhydroxy and the Cu2+ complexation in the tyrosine in the salvianolic acid molecule, and the similar of its structure and tryrosinase substrate, the para-position phenol structure is all arranged, can with the tryrosinase competitive binding, thereby weakened the catalysed oxidn of tryrosinase to tyrosine and serial oxidation product thereof, suppressed the generation of melanocyte, play the effect of whitening.Because the similar such as salviol acid A, salvianolic acid B, salvianolic acid C all belong to phenolic acids, total phenolic acid that these monomeric compounds and these single this chemical compounds consist of has identical effect, namely can also can use the monomer whose chemical compound with total phenolic acid in an application of the invention.
Salvianolic acid can use separately as tyrosinase inhibitor, also can use with other compound, and contain pharmaceutic adjuvant or the conventional pharmaceutical carrier that pharmaceutically allows, as suppressing melanogenic skin-lightening cosmetic.Salvianolic acid of the present invention can also add other whitening agent compositions, and these whitening agent compositions can adopt but be not limited to following ingredients: arbutin, kojic acid derivative, L-AA and derivant thereof and their salt, hydroquinone and derivant thereof and their salt; Plant extract such as Radix Glycyrrhizae extract, Aloe extract etc.The whitening agent composition can select arbitrarily one kind or two or more mixing.
Experimental results show that salvianolic acid has the promotion cell-proliferation activity, salvianolic acid can be used as skin care, the crease-resistant factor is used in cosmetics.One of performance of skin aging is exactly the multiplication capacity reduction of epidermis cell, the speed of skin cell renewal is slower than aging dead speed, makes thinning of skin, and then wrinkle occurs, because salvianolic acid has the promotion cel l proliferation, thereby salvianolic acid has skin care, crease-resistant effect.
Salvianolic acid is used in preparation medicine, cosmetics, wherein the visual different purposes of the use amount of salvianolic acid and difference.
When preparation medicine or cosmetics, with the pharmaceutical carrier of salvianolic acid with routine, make the external preparation such as Emulsion or ointment such as ointment base, emulsifying agent, surfactant, stabilizing agent etc., preparation technology can adopt conventional pharmaceutical preparation progression.Salvianolic acid in cosmetics or the consumption of medicine be 0.01～10% more suitable by weight percentage.
Tryrosinase has participated in the browning reaction of many fruit and vegerable fish and shrimp, is that these fruit and vegerable fish and shrimp brownings are given birth to speckle and lost bright main cause.And salvianolic acid has tyrosinase inhibitory action, can use in the food additive of prevention brown stain.
Beneficial effect of the present invention
The invention provides the salvianolic acid that from Radix Salviae Miltiorrhizae, extracts as the new purposes of tyrosinase inhibitor.By it is suppressed active mensuration, prove the active strong of salvianolic acid, and have preferably stability.Salvianolic acid also can significantly reduce the activity of tryrosinase in the melanoma cells, reduces the biosynthesis level of melanocyte in the cell, and salvianolic acid has under low concentration and promotes preferably cel l proliferation.
The specific embodiment
The present invention is further described below in conjunction with the implementation example, but embodiment does not limit protection scope of the present invention.
Embodiment 1:
Precision takes by weighing L-dopa 0.05g, with 0.1mol/L PBS (pH6.8) dissolving, is settled to 10mL, lucifuge, ultrasonic dissolution.Matching while using is answered in the easy oxidation of L-dopa solution.
The tryrosinase powder of 25KU is dissolved with 0.1mol/L PB (pH6.8), be settled to 250mL, the tryrosinase solution for preparing is divided be filled in the centrifuge tube, sealing is placed on-20 ℃ of Refrigerator stores.
Precision takes by weighing salviol total acid 10.00mg, dissolves to get salvianolic acid solution with the 10mL distilled water.By the distilled water stepwise dilution, finally obtaining extract concentrations is three concentration groups of 1.0mg/mL, 0.1mg/mL, 0.01mg/mL.4 ℃ save backup
Frozen tryrosinase solution places 37 ℃ of water-baths, rocks fast to make that solution melts rapidly in the pipe, and each tryrosinase solution that melts must be finished in 4h.
Press table 1, accurately draw sample, 0.1mol/LPB (pH6.8), 0.5%L-dopa, abundant mixing in 96 orifice plates, establish 4 multiple holes for every group, 37 ℃ of water-bath 20min add 100U/mL tryrosinase solution, hatch 10min for 37 ℃, measure absorbance (the results are shown in Table 2) at the 490nm place.
The composition of table 1 liquid to be measured
Tyrosinase inhibition rate (I%) computing formula is as follows:
A: the absorbance that enzyme mixation is surveyed that adds that does not add sample;
B: do not add the also absorbance surveyed of not enzyme-added mixed liquor of sample;
C: add the absorbance that the mixed liquor of sample and enzyme is surveyed;
D: add sample and absorbance that not enzyme-added mixed liquor is surveyed;
The suppression ratio of table 2 salvianolic acid under each concentration
The result shows: salvianolic acid all has inhibitory action three concentration to tyrosinase activity.
Implementation example 2:
Salvianolic acid is on the impact of A375 and Hacat co-cultured cell tyrosinase activity
To be in A375 cell and the Hacat cell of exponential phase, respectively through 0.25% trypsinization, centrifugal, MEM culture fluid suspendible.In 1: 2 ratio two kinds of cell suspension are mixed, adjusting cell density is 1.5 * 10
4Individual/mL is seeded in 96 orifice plates, and every hole 200 μ L are at 37 ℃ and 5%CO
2Under hatch 24h.The back-off culture fluid, choose 4 concentration (400 μ g/mL, 200 μ g/mL, 100 μ g/mL, 50 μ g/mL) according to the MTT experimental result, every hole 200 μ L, establish 6 multiple holes for every group, continue to hatch 48h, PBS washing 2 times, every hole adds 1%Triton-X solution 100 μ L, put rapidly and place 30min in-80 ℃ of refrigerators, room temperature is melted after taking out, and makes the abundant cracking of cell.Every hole adds 0.5%L-DOPA solution 50 μ L, and culture plate is placed the concussion of microwell plate oscillator, and 37 ℃, concussion 3h, 490nm wavelength place measures each hole OD value.The active A490 value representation of using of TYR.TYR maximum inhibition=[(experimental group OD value-blank group OD value)/(negative control group OD value-blank group OD value)] * 100% (the results are shown in Table 3).
Table 3 salvianolic acid is on the impact on A375 and Hacat co-cultured cell tyrosinase activity
The result shows: salvianolic acid all has inhibitory action four concentration to the intracellular tyrosine enzymatic activity, and is active strong during low concentration.
Implementation example 3:
Salvianolic acid is on A375 and the synthetic impact of Hacat co-cultured cell melanocyte
A375 cell and 1: 2 ratio of Hacat cell are mixed, and adjusting cell density is 4.5 * 10
4Individual/mL is seeded in 6 orifice plates, and every hole 2mL is at 37 ℃ and 5%CO
2Under hatch 24h.Culture fluid is abandoned in suction, choose 4 concentration (400 μ g/mL, 200 μ g/mL, 100 μ g/mL, 50 μ g/mL according to the MTT experimental result, every hole 2mL, continue to hatch 48h, remove culture fluid, clean 2 times with PBS, inhale and abandon culture fluid, add the digestion of 0.25% pancreatin 1mL/ hole after, add the 1mLMEM culture fluid and end digestion, make cell suspension, cell suspension is collected in the 15mL centrifuge tube, centrifugal, abandon supernatant, adding 100 μ L concentration is 1mol/LNaOH, 37 ℃ of water-bath 1h, fully cell lysis and dissolving melanin granule.Add 400 μ L distilled waters and release rarely, fully behind the mixing, add to respectively 96 orifice plates with 100 μ L/ holes, establish 4 multiple holes, measure each hole OD value at the 490nm wavelength for every group.Melanin content is with A490 value representation (the results are shown in Table 4).Melanin content=[(meansigma methods of each concentration absorbance/cell number of experimental group)/(meansigma methods of negative control group absorbance/cell number)] * 100%.
Table 4 salvianolic acid is on A375 and the synthetic impact of Hacat co-cultured cell melanocyte
The result shows: salvianolic acid synthesizes melanocyte 4 concentration all inhibitory action.
Implementation example 4:
Salvianolic acid is on the impact of A375 and Hacat co-cultured cell propagation
To be in A375 cell and the Hacat cell of exponential phase, respectively through 0.25% trypsinization, centrifugal, resuspended, the cell counting of MEM culture fluid.In 1: 2 ratio two kinds of cell suspension are mixed, adjusting cell density is 1.5 * 10
4Individual/mL is seeded in 96 orifice plates, and every hole 200 μ L are at 37 ℃ and 5%CO
2Under hatch 24h.Abandon culture fluid, by experiment grouping administration, every hole 200 μ L establish 6 multiple holes, continue to hatch 48h for every group.Cultivate and finish front 4h, every hole adds the MTT 20 μ L of 5g/L, and after continuing to cultivate 4h, method of reverse deduction is removed culture fluid, adds 150 μ L DMSO, and culture plate is placed the concussion of microwell plate oscillator, and 37 ℃, 10min makes dissolving crystallized.Microplate reader 490nm measures absorbance.Cell-proliferation activity is with A490 value representation (the results are shown in Table 5).Cell proliferation rate=(experimental group OD value-blank group OD value)/(negative control group OD value-blank group OD value) * 100%
Table 5 salvianolic acid is on the impact of A375 and Hacat co-cultured cell propagation
The result shows: salvianolic acid has under low concentration and promotes preferably cel l proliferation.
Claims (9)
1. salvianolic acid is in the application of restraint of tyrosinase activity.
2. according to claims 1 described application, wherein said salvianolic acid comprises Radix Salviae Miltiorrhizae total phenolic acids, salviol acid A, salvianolic acid B, salvianolic acid C.
3. according to claims 1 described purposes, it is characterized in that the application of salvianolic acid in the medicines such as skin splash, chloasma and freckle that preparation is caused by tryrosinase.
4. according to claims 1 described purposes, it is characterized in that the application of salvianolic acid in the biosynthetic whitening cosmetics of preparation inhibition melanocyte.
5. salvianolic acid is characterized in that in the application that promotes cell-proliferation activity salvianolic acid is applied in the cosmetics as the crease-resistant skin-protecting factor of skin care.
6. according to claims 3 described purposes, it is characterized in that, be added with at least a in wetting agent, emulsifying agent, excipient, stabilizing agent, surfactant, pH adjusting agent, the carrier in its described medicine.
7. according to claims 4 and 5 described purposes, it is characterized in that its described cosmetics are a kind of form in solution, suspension, emulsion, cream, ointment, gel, milk, powder, fancy soap, oil, dry powder, foundation emulsion, wet-milling or the spray.
8. according to claims 7 described purposes, it is characterized in that, be added with at least a in oiliness composition, powder, pH adjusting agent, active ingredient, antiseptic, wetting agent, saccharide, antioxidant, other whitening agents, vitamins and the water in the described cosmetics.
9. 1 described purposes as requested is characterized in that, the application of salvianolic acid in the food additive of preparation prevention fruit and vegerable fish and shrimp variable color.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210450370.6A CN102895308B (en) | 2012-11-13 | 2012-11-13 | New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210450370.6A CN102895308B (en) | 2012-11-13 | 2012-11-13 | New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102895308A true CN102895308A (en) | 2013-01-30 |
| CN102895308B CN102895308B (en) | 2014-05-14 |
Family
ID=47567994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210450370.6A Active CN102895308B (en) | 2012-11-13 | 2012-11-13 | New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102895308B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104840380A (en) * | 2015-01-21 | 2015-08-19 | 青岛科技大学 | Natural substance composition and application of same in whitening cosmetic |
| CN105596322A (en) * | 2014-11-17 | 2016-05-25 | 兰赫(上海)生物科技有限公司 | Tyrosinase inhibitor and applications thereof |
| CN105662903A (en) * | 2014-11-17 | 2016-06-15 | 兰赫(上海)生物科技有限公司 | Skin whitening and brightening compound and application thereof |
| CN105662904A (en) * | 2014-11-17 | 2016-06-15 | 兰赫(上海)生物科技有限公司 | Melanogenesis inhibitor and applications thereof |
| CN106031708A (en) * | 2015-03-17 | 2016-10-19 | 天津药物研究院有限公司 | Pharmaceutical composition with effects of skin care, wrinkle removing, and anti-aging effect, and preparation method thereof |
| CN107141275A (en) * | 2017-06-09 | 2017-09-08 | 云南中烟工业有限责任公司 | A kind of arylbenzofuran class compound with antioxidation activity and its preparation method and application |
| CN107325068A (en) * | 2017-06-09 | 2017-11-07 | 云南中烟工业有限责任公司 | A kind of arylbenzofuran class compound and its preparation method and application |
| CN110763597A (en) * | 2019-07-31 | 2020-02-07 | 杭州曼迪生物科技有限公司 | Method for evaluating whitening effect of cosmetics based on transdermal absorption |
| CN116585335A (en) * | 2023-06-07 | 2023-08-15 | 广东植肤生物科技有限公司 | A skin external composition for inhibiting TYR activity and MC1R expression |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102629000B1 (en) * | 2016-09-06 | 2024-01-25 | 주식회사 엘지생활건강 | Composition for skin improvement containing Salvianolic acid C |
-
2012
- 2012-11-13 CN CN201210450370.6A patent/CN102895308B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 徐宝军，等: "天然产物增白作用的初步筛选", 《日用化学工业》 * |
| 赵娜，等: "丹参的化学成分与药理作用", 《国外医药.植物药分册》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105596322A (en) * | 2014-11-17 | 2016-05-25 | 兰赫(上海)生物科技有限公司 | Tyrosinase inhibitor and applications thereof |
| CN105662903A (en) * | 2014-11-17 | 2016-06-15 | 兰赫(上海)生物科技有限公司 | Skin whitening and brightening compound and application thereof |
| CN105662904A (en) * | 2014-11-17 | 2016-06-15 | 兰赫(上海)生物科技有限公司 | Melanogenesis inhibitor and applications thereof |
| CN104840380A (en) * | 2015-01-21 | 2015-08-19 | 青岛科技大学 | Natural substance composition and application of same in whitening cosmetic |
| CN106031708A (en) * | 2015-03-17 | 2016-10-19 | 天津药物研究院有限公司 | Pharmaceutical composition with effects of skin care, wrinkle removing, and anti-aging effect, and preparation method thereof |
| CN107141275A (en) * | 2017-06-09 | 2017-09-08 | 云南中烟工业有限责任公司 | A kind of arylbenzofuran class compound with antioxidation activity and its preparation method and application |
| CN107325068A (en) * | 2017-06-09 | 2017-11-07 | 云南中烟工业有限责任公司 | A kind of arylbenzofuran class compound and its preparation method and application |
| CN107141275B (en) * | 2017-06-09 | 2019-08-06 | 云南中烟工业有限责任公司 | A kind of arylbenzofuran class compound and its preparation method and application with antioxidant activity |
| CN107325068B (en) * | 2017-06-09 | 2020-08-25 | 云南中烟工业有限责任公司 | Aryl benzofuran compound and preparation method and application thereof |
| CN110763597A (en) * | 2019-07-31 | 2020-02-07 | 杭州曼迪生物科技有限公司 | Method for evaluating whitening effect of cosmetics based on transdermal absorption |
| CN116585335A (en) * | 2023-06-07 | 2023-08-15 | 广东植肤生物科技有限公司 | A skin external composition for inhibiting TYR activity and MC1R expression |
| CN116585335B (en) * | 2023-06-07 | 2024-02-09 | 广东植肤生物科技有限公司 | A skin external composition for inhibiting TYR activity and MC1R expression |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102895308B (en) | 2014-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102895308B (en) | New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle | |
| CN102440928B (en) | Traditional Chinese medicine composition and preparation with skin microcirculation promotion function and preparation method thereof | |
| CN102008412B (en) | Skin care composition with whitening efficacy, and preparation method thereof | |
| CN101836944B (en) | Composition of plant extracts and application in skin whitening and moisture preservation | |
| CN110051572A (en) | Pomegranate fermentation material and application thereof | |
| CN113694115A (en) | Application of Fuzhuan tea extract in preparation of skin conditioning product | |
| CN105381007A (en) | Externally-applied traditional Chinese medicine composition with effect on whitening skin, preparation as well as preparation method and application thereof | |
| CN103142435A (en) | Whitening composition inhibiting tyrosinase activities with multiple mechanisms, and products of composition | |
| CN102232917A (en) | Use of traditional Chinese drug compound extract in preparing whitening and moisturizing products | |
| CN101450047B (en) | Use of black mulberry extract as tyrosinase inhibitor | |
| KR20130039145A (en) | Composition for preventing hair loss or promoting hair growth comprising extracts of panax ginseng and hijikia fusiforme | |
| CN102670978B (en) | A kind of polygonatum compound granule and preparation method thereof | |
| EP2596787B1 (en) | New use of hesperetin | |
| JP2010235482A (en) | Galenical extract and collagen production promoter using the same, and fibroblast activator | |
| CN103860436B (en) | Cosmetic composition containing schisandra chinensis extract and nourishing whitening cream | |
| CN110227088A (en) | Composition for external application with itching-relieving efficacies | |
| CN103784347A (en) | Anti-rhagades hand cream | |
| CN104958196A (en) | Application of golden flower tea extract, skin whitening and caring composition and preparing method of skin whitening and caring composition | |
| CN110496074A (en) | A kind of plant anti-acne maintenance Essence | |
| JP2005239660A (en) | Precursor lipocyte differentiation promoter | |
| JP2009184997A (en) | Collagen production promoter | |
| KR101528448B1 (en) | A composition for improving skin conditions containing salvianolic acid B | |
| CN106166123A (en) | There is the skin care compositions of antiallergic itching-relieving efficacies, emulsion and preparation method thereof | |
| JP2014040487A (en) | Fat accumulation inhibitor | |
| CN114788796B (en) | Whitening and antibacterial cosmetic containing Fuding white tea extract and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200729 Address after: No.13 building, innovation and entrepreneurship square, science and technology innovation city, Harbin high tech Industrial Development Zone, Heilongjiang Province d836, torch Eurasian building, 72 Shimao Avenue Patentee after: Heilongjiang Yuanmeng Health Industry Development Co., Ltd Address before: 150040 building 24, Heping Road, Heping District, Heilongjiang, Harbin 1610, Xiangfang Patentee before: Sun Haifeng |
|
| TR01 | Transfer of patent right |