CN102893852B - Cultivating method of high-digestibility forage sorghum - Google Patents
Cultivating method of high-digestibility forage sorghum Download PDFInfo
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- CN102893852B CN102893852B CN 201210298518 CN201210298518A CN102893852B CN 102893852 B CN102893852 B CN 102893852B CN 201210298518 CN201210298518 CN 201210298518 CN 201210298518 A CN201210298518 A CN 201210298518A CN 102893852 B CN102893852 B CN 102893852B
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- sorghum
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- digestibility
- arteries
- cinnamic aldehyde
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Abstract
According to the invention, brown midrib sorghum breeds with high cinnamic aldehyde content (high digestibility) are screened with a chemical analysis method, and are subjected to repeated backcrossing with common sorghum breeds; cinnamic aldehyde contents of backcrossing progenies are continuously improved; and a forage sorghum novel breed with high digestibility is obtained. When the cultivating method with cinnamic aldehyde as an index is compared with a method for visually observing the shades of brown midrib colors, research processes and results are more quantitative and accurate. When the cultivating method with cinnamic aldehyde as an index is compared with a zootechnical farming method, the operation is simple and the method is highly efficient.
Description
Technical field
The invention belongs to improved crop variety cultivation technology, specifically the selection of a kind of high digestibility forage sorghum.
Background technology
Arteries and veins is that a kind of vein and cane xylem present brown ash or tan gene mutation phenomenon in the Chinese sorghum brown.This variant is compared with the Chinese sorghum of common White midrib, its content of lignin that is difficult to digest has reduced 40%-60%, and digestibility increases substantially, and palatability is obviously improved, various pasture stocks are liked food especially, and same scale of feeding can obtain higher economic benefit.Studies show that, the shade of arteries and veins becomes positive correlation with digestibility in the brown, and namely the darker digestibility of color is higher.But be subjected to impact impure in the Chinese sorghum different cultivars during with the eyes comparison, the depth of middle arteries and veins brown often difficulty is distinguished relative superiority or inferiority.Therefore, the mensuration comparative approach that needs a kind of science.The invention solves this problem.Because the brown of arteries and veins includes the pigment cinnamic acid by it and causes in the Chinese sorghum, cinnamic aldehyde content is high, and the brown of arteries and veins is just dark in the Chinese sorghum, and digestibility is just high.We are applied to the assay method of Chinese sorghum cinnamic aldehyde content the prevalent variety cultivation of high digestibility forage sorghum, can make content, the progress of research realize quantitative and accurate.
The digestibility research of livestock breeding is a very complicated job, not only wants letting animals feed, and will measure the input of feed every day, also will collect, the excreta of analyzing animal.If come in this way the forage sorghum of the high digestibility of seed selection, workload great is bound to arouse fear.Be the forage sorghum kind of the high digestibility of index seed selection with cinnamic aldehyde content, simple to operation, the result quantitatively, precisely, better effects if.
Summary of the invention
The objective of the invention is assay method with Chinese sorghum cinnamic acid pigment and be used for and cultivate both high yield and high quality, digestibility is high forage sorghum new varieties again.
Basic ideas of the present invention: the height of Chinese sorghum digestibility is proportionate with the depth of arteries and veins brown wherein, and the depth of this color is decided by the height of its pigment cinnamic aldehyde content.As long as so record cinnamic aldehyde content, just can determine quantitatively the depth of middle arteries and veins brown, also just can learn the height of its plant digestibility.That is to say, can determine by the height of cinnamic aldehyde content the height of sorghum variety digestibility.In breeding work, the material that our direct seed selection cinnamic aldehyde content is high just can find the high germplasm of digestibility.On this basis, we select the high Chinese sorghum of cinnamic aldehyde content and common Chinese sorghum repeatedly to backcross, and cultivate at last the forage sorghum new varieties of high yield and high quality, high digestibility.
The present invention is achieved in that
1. select the Parents In Sorghum of high digestibility:
1. the arteries and veins Chinese sorghum is long to 1 meter height in the brown, and the vein of Chinese sorghum is taken off.
2. at once getting vein is sheared the segment about growth 1cm, placed 50 ml centrifuge tubes.
The fresh middle arteries and veins adding 10ml concentration of 3. getting 1g is the NaOH solution of 1mol/L.
4. centrifuge tube is placed on the shaking table, rotating speed is 200rpm, and normal temperature concussion 48 hours is turned white to middle arteries and veins color, and extraction is finished.
5. take the NaOH of 1mol/L as contrast, under the wavelength of 330nm, measure the absorbance of extract with ultraviolet specrophotometer.
6. choose the Chinese sorghum of absorbance large (be that cinnamic aldehyde content is high, digestibility is high) as the parent of crossbreeding.
2. high digestibility proterties is backcrossed and changes the sorghum variety of high yield and high quality over to:
1. choose the common sorghum variety of current good quality and high output.
2. the Sorghum Hybrid that common Chinese sorghum and the cinnamic aldehyde content selected is high.
3. selfing after the hybridization, high individual plant and the Sudan grass of arteries and veins cinnamic aldehyde content backcrosses in the self progeny selects again.
4. backcross again 2 times by step 3, all need selfing after backcrossing at every turn.
5. at last again selfing once namely obtain to change over to the Sudan. grass Varieties of high digestibility proterties.
Illustrate: because the arteries and veins gene is recessive gene in the brown, all need selfing after backcrossing, to show its proterties at every turn.
Benefit of the present invention:
1. utilize the analytical chemistry means to measure, and visually observe pulse-phase ratio in the brown, the process of research work and result are become quantitatively by qualitative, and accurately and reliably.
2. utilize the high digestibility sorghum variety of cinnamic aldehyde content index seed selection, workload is less, and operation is simple, gets twice the result with half the effort, and drives numerously with letter, and the cost saving effect is grand.
3. the technology of the present invention high digestibility breeding of new variety of also can be used for Sudan grass, the Gao Dancao of high digestibility and changing other forage grass plant of arteries and veins gene in the brown over to.
Embodiment
Embodiment 1. the forage sorghum that seed selection output is high, digestibility is high:
1. select the Parents In Sorghum of high digestibility:
1. the arteries and veins Chinese sorghum is long to 1 meter height in the brown, and the vein of Chinese sorghum is taken off.
2. at once getting vein is sheared the segment about growth 1cm, placed 50 ml centrifuge tubes.
The fresh middle arteries and veins adding 10ml concentration of 3. getting 1g is the NaOH solution of 1mol/L.
4. centrifuge tube is placed on the shaking table, rotating speed is 200rpm, and normal temperature concussion 48 hours is turned white to middle arteries and veins color, and extraction is finished.
5. take the NaOH of 1mol/L as contrast, under the wavelength of 330nm, measure the absorbance of extract with ultraviolet specrophotometer.
6. choose the Chinese sorghum of absorbance large (be that cinnamic aldehyde content is high, digestibility is high) as the parent of crossbreeding.
2. high digestibility proterties is backcrossed and changes the sorghum variety of high yield and high quality over to:
1. choose the common sorghum variety of current good quality and high output.
2. the Sorghum Hybrid that common Chinese sorghum and the cinnamic aldehyde content selected is high.
3. selfing after the hybridization, high individual plant and the Sudan grass of arteries and veins cinnamic aldehyde content backcrosses in the self progeny selects again.
4. backcross again 2 times by step 3, all need selfing after backcrossing at every turn.
5. at last again selfing once namely obtain to change over to the Sudan. grass Varieties of high digestibility proterties.
Embodiment 2. breeding high-yields, the Pacetter varieties that digestibility is high:
Gao Dancao is the novel plant that produces after Chinese sorghum and the Sudan grass hybridization.To namely obtain arteries and veins Gao Dancao in the brown after the hybridization of arteries and veins Sudan grass in arteries and veins Chinese sorghum and the brown in the brown.
1. select arteries and veins Chinese sorghum in the high brown of digestibility
1. the arteries and veins Chinese sorghum is long to 1 meter height in the brown, and the vein of Chinese sorghum is taken off.
2. at once getting vein is sheared the segment about growth 1cm, placed 50 ml centrifuge tubes.
The fresh middle arteries and veins adding 10ml concentration of 3. getting 1g is the NaOH solution of 1mol/L.
4. centrifuge tube is placed on the shaking table, rotating speed is 200rpm, and normal temperature concussion 48 hours is turned white to middle arteries and veins color, and extraction is finished.
5. take the NaOH of 1mol/L as contrast, under the wavelength of 330nm, measure the absorbance of extract with ultraviolet specrophotometer.
6. choose the Chinese sorghum of absorbance large (be that cinnamic aldehyde content is high, digestibility is high) as the parent of crossbreeding.
2. the high digestibility Sudan grass of seed selection:
1. with Sudan grass and high digestibility Sorghum Hybrid.
2. selfing after the hybridization, high individual plant and the Sudan grass of arteries and veins cinnamic aldehyde content backcrosses in the self progeny selects.
3. the selfing after 1 time that backcrosses, high individual plant and the Sudan grass of arteries and veins cinnamic aldehyde content backcrosses in the self progeny selects again.
4. backcross again 2 times by step 3, all need selfing after backcrossing at every turn.
5. at last again selfing once namely obtain to change over to the Sudan. grass Varieties of high digestibility proterties
3. high digestibility Sudan grass and high digestibility Sorghum Hybrid obtain arteries and veins Pacetter varieties in the high brown of digestibility.
Claims (1)
1. the selection of a high digestibility forage sorghum comprises the steps:
(1)Select the Parents In Sorghum of high digestibility:
1. the arteries and veins Chinese sorghum is long to 1 meter height in the brown, and the vein of Chinese sorghum is taken off;
2. the fragment of at once getting vein being sheared about growth 1cm places 50 ml centrifuge tubes;
The fresh middle arteries and veins adding 10ml concentration of 3. getting 1g is the NaOH solution of 1mol/L;
4. centrifuge tube is placed on the shaking table, rotating speed 200rpm, normal temperature concussion 48 hours is turned white to middle arteries and veins color, and extraction is finished;
5. take the NaOH of 1mol/L as contrast, under the wavelength of 330nm, measure the absorbance of extract with ultraviolet specrophotometer;
6. choose absorbance and be greatly in the high brown of cinnamic aldehyde content the arteries and veins Chinese sorghum as the parent of crossbreeding;
(2)High digestibility proterties backcrossed changes the sorghum variety of high yield and high quality over to:
1. choose the common sorghum variety of current good quality and high output;
2. the Sorghum Hybrid that common Chinese sorghum and the cinnamic aldehyde content selected is high;
3. selfing after the hybridization, high individual plant and the common sorghum variety of arteries and veins cinnamic aldehyde content backcrosses in the self progeny selects;
4. the selfing after 1 time that backcrosses, high individual plant and the common sorghum variety of arteries and veins cinnamic aldehyde content backcrosses in the self progeny selects again;
5. backcross again 2 times by step 4, all need selfing after backcrossing at every turn;
6. at last again selfing once namely obtain the sorghum variety of high digestibility.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310480562.6A CN103988772B (en) | 2012-08-22 | 2012-08-22 | The breeding method of the high red grass crossing kind of a kind of high digestibility |
| CN 201210298518 CN102893852B (en) | 2012-08-22 | 2012-08-22 | Cultivating method of high-digestibility forage sorghum |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103305624B (en) * | 2013-07-07 | 2015-04-01 | 安徽科技学院 | Molecular identification method of high-digestibility sorghum strain |
| CN103299898A (en) * | 2013-07-07 | 2013-09-18 | 安徽科技学院 | Molecular marker-assisted cultivating method of new high-digestibility sudangrass strain |
| CN103960125B (en) * | 2014-05-19 | 2016-03-02 | 河北省农林科学院旱作农业研究所 | Green grass or young crops cuts the selection of class Sudan grass new germ plasm |
| CN110089422A (en) * | 2019-06-02 | 2019-08-06 | 辽宁省农业科学院 | A kind of breeding method improving amylopectin content in black sorghum seed |
| CN110679479A (en) * | 2019-11-15 | 2020-01-14 | 河北省农林科学院旱作农业研究所 | Hybrid breeding method of BMR genotype sudan grass |
| CN111543309A (en) * | 2020-05-19 | 2020-08-18 | 吉林省农业科学院 | Breeding method of pasture |
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| US8420906B2 (en) * | 2009-11-11 | 2013-04-16 | The Texas A&M University System | Inbred sorghum line ‘R07007’ |
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2012
- 2012-08-22 CN CN201310480562.6A patent/CN103988772B/en not_active Expired - Fee Related
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| CN103988772B (en) | 2016-05-25 |
| CN102893852A (en) | 2013-01-30 |
| CN103988772A (en) | 2014-08-20 |
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