CN110089422A - A kind of breeding method improving amylopectin content in black sorghum seed - Google Patents
A kind of breeding method improving amylopectin content in black sorghum seed Download PDFInfo
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- CN110089422A CN110089422A CN201910500543.2A CN201910500543A CN110089422A CN 110089422 A CN110089422 A CN 110089422A CN 201910500543 A CN201910500543 A CN 201910500543A CN 110089422 A CN110089422 A CN 110089422A
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- black sorghum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Genetics & Genomics (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention discloses a kind of breeding method for improving amylopectin content in black sorghum seed, including following operating procedure: black sorghum starch is made with mature black sorghum seed, then using branch/amylose ratio in the black sorghum starch of white oil by dual-wavelength method, maximum i.e. parent of the highest black sorghum of amylopectin content as crossbreeding of ratio is chosen；It using the black sorghum male sterile line of current good quality and high output as female parent, then with itself and the highest black Sorghum Hybrid of content, is selfed after hybridization, the highest black sorghum single plant of amylopectin content and above-mentioned maternal backcrossing is selected in self progeny；Selfing after backcrossing is primary, then select amylopectin content highest single plant and the black sorghum male sterile line female parent of current good quality and high output to be returned in the offspring of selfing；After repeating 5-6 times, all it is selfed after backcrossing every time.Breeding method provided by the invention, breeding speed is fast, can precisely be effectively obtained the black sorghum variety of high amylopectin starch content.
Description
Technical field
The invention belongs to Sorghum Breeding technical fields, and in particular to a kind of to improve amylopectin content in black sorghum seed
Breeding method.
Background technique
Black sorghum is perennial herb, and plant is tall and big, reaches 2-3m high, branch is less, stem thickness 0.5-1.0cm, blade the wide line
Property, long 30-50cm, wide 2-5cm, base portion has a white tiny stream shape hair, middle arteries white and thick, and panicle is carried out, it is about 50-60cm,
Pale red is to atropurpureus, and small ear is twin, and one piece has handle, and another piece of stockless, having handle, person is male or infertility of degenerating, stockless small ear two
Property, it can be solid, Sccded spikclct is needle-shaped in draping over one's shoulders, and middle part is wider, and seed is shorter；A length of 6mm of small ear or so, width is 2.5mm or so, thick
1.8mm or so, clever hard leather matter show atropurpureus mostly, and smooth surface is glossy；After small ear is mature, rolled over mostly from from rachis internode
Disconnected, easy band stays cob segment, and fractureing, it is irregular to locate.The outside of belly is often with the rachilla 1-2 being broken off；From pass after only a few small ear is mature
Section falls off, and falling off, it is neat to locate, and the outside of belly has the articulate rachilla of 1-2.Original frequently with black sorghum as wine brewing in the prior art
Material, the influence about wine brewing sorghum material to Wine-making quality, liquor output rate, relevant report are less.Black sorghum ingredient includes starch (branch
Chain starch, amylose), protein, lysine, fat, tannin etc., main component of the starch as wine brewing account for about sorghum
65-70%, followed by protein account for about 8-11%, and crude fat accounts for 3%, crude fibre 2-3%.The ingredient of different cultivars sorghum contains
Measure different, the quality of their contents and distillation yield and wine has close relationship.Some breweries are investigated, discovery is containing shallow lake
Meal component height especially amylopectin is high, and protein, tannin content are moderate, and the less sorghum of fat is the ideal original of wine brewing
Material.Therefore in order to change the content of amylopectin in existing black sorghum, it is badly in need of a kind of improving amylopectin in black sorghum seed
The breeding method of content.
Summary of the invention
In order to promote the content of wine brewing amylopectin in black sorghum, the black sorghum product of the content of high amylopectin starch are obtained
Kind, the present invention provides a kind of breeding method for improving amylopectin content in black sorghum seed.
The present invention is achieved by the following technical solutions.
A kind of breeding method improving amylopectin content in black sorghum seed, including following operating procedure:
A: the Parents In Sorghum of selection high amylopectin starch content
Black sorghum starch is made with mature black sorghum seed, then using branch in the black sorghum starch of white oil by dual-wavelength method/
Amylose ratio chooses maximum i.e. parent of the highest black sorghum of amylopectin content as crossbreeding of ratio；
B: the black sorghum variety of high amylopectin starch content obtains
(1) using the black sorghum male sterile line of current good quality and high output as female parent, then with itself and the highest black height of content
Fine strain of millet hybridization, is selfed after hybridization, and the highest black sorghum single plant of amylopectin content and above-mentioned maternal backcrossing are selected in self progeny；
(2) selfing after backcrossing is primary, then select in the offspring of selfing amylopectin content highest single plant with it is currently high-quality
The black sorghum male sterile line female parent of high yield is returned；
(3) it after repetition step (2) 5-6 times, is all selfed after backcrossing every time, high branch shallow lake is obtained after finally selfing is primary
The black sorghum variety of powder content.
Specifically, above-mentioned black sorghum seed prepares the concrete operations of black sorghum starch are as follows: black sorghum seed is soaked in water 5
It after hour, grinds, after filtering, filtrate stands, the starch petroleum ether degreasing that will be obtained, then after washing 2 times, uses 0.05mol/L
Sodium hydroxide solution elution three times, after being then neutral to pH value wash with distilled water, starch is dried, is crossed after 120 mesh
To sorghum starch.
Specifically, in above-mentioned steps B, field method used by all selfings, hybridization is as follows: trench digging drilling, line-spacing
50cm, test area surrounding setting protection row；Two weeks emergence, sows the weeding of the latter moon, thinning, is left to seedling spacing 10-
12cm, breeding time irrigate in due course, apply fertilizer.
From the above technical scheme, it can be seen that the beneficial effects of the present invention are:
(1) using branch/amylose ratio in the black sorghum seed of chemical gauging, measurement result is accurate, facilitates essence
The high black Parents In Sorghum of quasi- selection amylopectin content, shortens the period of breeding and the effect of breeding；
(2) the black sorghum of the final obtained high amylopectin starch content of the present invention, the total starch content in seed can achieve
80% or so, wherein branch/straight starch ratio can achieve 3.6 or so, the quality of black sorghum is effectively improved, wine is finally improved
The effect of wine.
Specific embodiment
In order to which objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
It is described in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Embodiment 1
A kind of breeding method improving amylopectin content in black sorghum seed, including following operating procedure:
A: the Parents In Sorghum of selection high amylopectin starch content
Black sorghum starch is made with mature black sorghum seed, then using branch in the black sorghum starch of white oil by dual-wavelength method/
Amylose ratio chooses maximum i.e. parent of the highest black sorghum of amylopectin content as crossbreeding of ratio；
B: the black sorghum variety of high amylopectin starch content obtains
(1) using the black sorghum male sterile line of current good quality and high output as female parent, then with itself and the highest black height of content
Fine strain of millet hybridization, is selfed after hybridization, and the highest black sorghum single plant of amylopectin content and above-mentioned maternal backcrossing are selected in self progeny；
(2) selfing after backcrossing is primary, then select in the offspring of selfing amylopectin content highest single plant with it is currently high-quality
The black sorghum male sterile line female parent of high yield is returned；
(3) it after repeating step (2) 6 times, is all selfed after backcrossing every time, obtains high amylopectin starch after finally selfing is primary
The black sorghum variety of content.
Specifically, above-mentioned black sorghum seed prepares the concrete operations of black sorghum starch are as follows: black sorghum seed is soaked in water 5
It after hour, grinds, after filtering, filtrate stands, the starch petroleum ether degreasing that will be obtained, then after washing 2 times, uses 0.05mol/L
Sodium hydroxide solution elution three times, after being then neutral to pH value wash with distilled water, starch is dried, is crossed after 120 mesh
To sorghum starch.
Specifically, in above-mentioned steps B, field method used by all selfings, hybridization is as follows: trench digging drilling, line-spacing
50cm, test area surrounding setting protection row；Two weeks emergence, sows the weeding of the latter moon, thinning, is left to seedling spacing 12cm,
Breeding time irrigates in due course, applies fertilizer.
Comparative example 1
The number that step (3) in embodiment 1B step repeats step (2) is changed to 3 times, remaining operating procedure and implementation
Example 1 is identical.
Black sorghum seed is made with the method for each embodiment and comparative example respectively, the starch then tested in seed always contains
Amount, branch/amylose ratio, wherein control group is existing black sorghum seed, and test result is as shown in table 1:
The content of starch of the black sorghum seed of table 1 measures
| Project | Total starch, % | Amylopectin, % | Amylose, % | Branch/straight starch ratio |
| Embodiment 1 | 80.5 | 61.4 | 21.1 | 2.9 |
| Comparative example 1 | 74.1 | 51.2 | 24.9 | 2.1 |
| Control group | 60.9 | 42.1 | 18.8 | 2.2 |
As shown in Table 1, the content of starch in black sorghum seed produced by the present invention is high, and amylopectin content is high, final to make
Wine quality it is good,
Certainly, the above description is not a limitation of the present invention, and the present invention is also not limited to the example above, the art
Those of ordinary skill, within the essential scope of the present invention, variation, change, addition or the replacement made all should belong to the present invention
Protection scope.
Claims (3)
1. a kind of breeding method for improving amylopectin content in black sorghum seed, which is characterized in that including following operating procedure:
A: the Parents In Sorghum of selection high amylopectin starch content
Black sorghum starch is made with mature black sorghum seed, then using branch/straight chain in the black sorghum starch of white oil by dual-wavelength method
Starch ratio chooses maximum i.e. parent of the highest black sorghum of amylopectin content as crossbreeding of ratio；
B: the black sorghum variety of high amylopectin starch content obtains
(1) then miscellaneous with the highest black sorghum of content with it using the black sorghum male sterile line of current good quality and high output as female parent
It hands over, is selfed after hybridization, the highest black sorghum single plant of amylopectin content and above-mentioned maternal backcrossing are selected in self progeny；
(2) selfing after backcrossing is primary, then amylopectin content highest single plant and current good quality and high output are selected in the offspring of selfing
Black sorghum male sterile line female parent be returned；
(3) it after repetition step (2) 5-6 times, is all selfed after backcrossing every time, obtains high amylopectin starch after finally selfing is primary and contain
Measure black sorghum variety.
2. a kind of breeding method for improving amylopectin content in black sorghum seed according to claim 1, feature exist
In above-mentioned black sorghum seed prepares the concrete operations of black sorghum starch are as follows: after black sorghum seed is soaked in water 5 hours, grind,
After filtering, filtrate is stood, the starch petroleum ether degreasing that will be obtained, then after washing 2 times, molten with the sodium hydroxide of 0.05mol/L
Liquid elutes three times, and after being then neutral to pH value wash with distilled water, starch is dried, and obtains sorghum starch after crossing 120 mesh.
3. a kind of breeding method for improving amylopectin content in black sorghum seed according to claim 1, feature exist
In in above-mentioned steps B, field method used by all selfings, hybridization is as follows: trench digging drilling, line-spacing 50cm, test area
Surrounding setting protection row；Two weeks emergence, sows the weeding of the latter moon, thinning, is left to seedling spacing 10-12cm, breeding time is in due course
It irrigates, fertilising.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893852A (en) * | 2012-08-22 | 2013-01-30 | 李杰勤 | Cultivating method of high-digestibility forage sorghum |
| CN103091273A (en) * | 2013-01-25 | 2013-05-08 | 贵州大学 | Method for rapidly determining starch content of sorghum grains |
| US20140215647A1 (en) * | 2013-01-31 | 2014-07-31 | Monsanto Technology Llc | Plants and seeds of sorghum variety gsv370669 |
| CN107197773A (en) * | 2017-07-06 | 2017-09-26 | 山西省农业科学院高粱研究所 | A kind of north glutinous sorghum sterile line and the selection of maintainer |
-
2019
- 2019-06-02 CN CN201910500543.2A patent/CN110089422A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893852A (en) * | 2012-08-22 | 2013-01-30 | 李杰勤 | Cultivating method of high-digestibility forage sorghum |
| CN103091273A (en) * | 2013-01-25 | 2013-05-08 | 贵州大学 | Method for rapidly determining starch content of sorghum grains |
| US20140215647A1 (en) * | 2013-01-31 | 2014-07-31 | Monsanto Technology Llc | Plants and seeds of sorghum variety gsv370669 |
| CN107197773A (en) * | 2017-07-06 | 2017-09-26 | 山西省农业科学院高粱研究所 | A kind of north glutinous sorghum sterile line and the selection of maintainer |
Non-Patent Citations (4)
| Title |
|---|
| 何照范: "《粮油籽粒品质及其分析技术》", 31 July 1985 * |
| 卢超 等: "高产高淀粉酿酒型高粱杂交种豫粱7号的选育", 《河南农业科学》 * |
| 彭秋 等: "优质酿造糯高粱黔高８号的选育及推广应用", 《贵州农业科学》 * |
| 王艳秋 等: "适于机械化收获优质酿造高粱杂交种辽杂50选育及栽培技术", 《辽宁农业科学》 * |
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