CN102845585A - Polyethylene glycol-microwave assisted almond protein extraction method - Google Patents
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Abstract
The invention relates to a polyethylene glycol-microwave assisted almond protein extraction method, which adopts degreased almond powder as a raw material, and makes use of polyethylene glycol-microwave assisted extraction, isoelectric point-ethanol co-precipitation and vacuum freeze-drying to extract almond protein. Polyethylene glycol is a novel green solvent, has a solubilization effect on effective ingredients of almond protein, can improve the leaching efficiency and the extraction rate, and has no toxicity. Microwave-assisted extraction has the characteristics of high thermal efficiency, rapid and uniform temperature rising, greatly improved almond protein extraction efficiency, as well as shortened extraction time. Moreover, the almond protein has the advantages of high purity, high precipitation rate, simple extraction method, short extraction time, and environmental friendliness.
Description
Technical field
The present invention relates to a kind of extracting method of almond protein, especially relate to a kind of method that adopts Microwave-assisted Extraction to get almond protein take polyethylene glycol as solvent.
Background technology
Almond is divided into two kinds of dessert almond and semen armeniacae amaraes, and semen armeniacae amarae is mainly as drug use, and dessert almond is then direct-edible or be used for food processing.Its flavor delicate fragrance, nutritive value of almond is very high, the mineral element that contains the multiple needed by human body such as rich in protein, sugar, crude fibre, calcium, phosphorus, iron, wherein, almond protein content reaches 24%～25%, 18 seed amino acids that contain needed by human body, A wide selection of colours and designs can be used as a kind of proteinaceous nutrient hardening agent and additive.The amino acid that contains 8 kinds of needed by human in the almond, the deficiency in can balanced diet.Studies show that almond has good medical effect, cure mainly chill tuberculosis, can promote the production of body fluid to quench thirst, moistening lung for removing phlegm and clearing heat and detoxicating etc., ediblely can give protection against cancer for a long time, anticancer, build up resistance, delay senility useful health of heart and brain.In addition, especially almond protein has antihypertensive activity.Therefore, almond protein is a kind of protein resource of high-quality, and its development and use are significant to the functional health of human body.
Extracting method about vegetable protein mainly contains following several at present:
(1) traditional protein extracting method is to utilize the strong base solution lixiviate.The method mainly is to utilize the strong base solutions such as NaOH that protein is extracted, it normally puts into aqueous slkali lixiviate certain hour (also can repeat for several times lixiviate) in the defatted apricot kernel powder, supernatant is taken out, regulate its pH value to isoelectric points of proteins with certain density acid solution, albumen precipitation is got off, and centrifugation, freeze drying get final product.The protein color and luster that the method is extracted is darker, needs the time to be processed long, and recovery rate is low, wastes a large amount of alkali lye, produces a large amount of waste water, causes environmental pollution.
(2) salt is carried: utilize the salting liquid lixiviate of low concentration, the method mainly is to utilize the salting liquids such as NaCl that protein is extracted, normally in the defatted apricot kernel powder, put into the salting liquid lixiviate certain hour (also can repeat for several times lixiviate) of low concentration, supernatant is taken out, regulate its pH value to isoelectric points of proteins with certain density acid solution, albumen precipitation is got off, centrifugation, freeze drying get final product, it is long that but the method is extracted required time, and recovery rate is lower.
(3) reverse micelle method extracts: in reverse micelle system, oversaturated surfactant dissolves is in non-polar solven the time, the nonpolar tail of surfactant contacts with organic solvent outside, polar group forms an inner surface inwardly, consists of a polarity core " pond " with equilibrium ion and water.When almond powder was dissolved in the reverse micelle system, protein was solubilized in the polarity pond of reverse micelle, and oil extraction enters in the organic solvent simultaneously, changed the condition of system water, such as pH, ionic strength etc., made protein return to water by organic phase.Subsequently organic phase cooling or column chromatography for separation go out surfactant, separated organic substance and grease.Lower floor's protein liquid is through the refining protein product that obtains.But the method complex process, extraction time is long, and recovery rate is low, and the use of organic solvent on protein nutritive value and edible safety impact, produces pollution to environment.
(4) aqueous enzymatic extraction: use proteinase hydrolization method to extract protein, though the method can improve the recovery rate of protein, but extraction cost is higher, and product is take almond peptide as main, the emulsification of albumen and the functional reduction of bubbling.
In sum, present almond protein extracting method all has certain deficiency, and the research of the extracting method that a kind of extraction process is simple, cost is low, recovery rate is high, purity is high is very necessary.
Summary of the invention
The object of the invention is to overcome the existing deficiency of the extracting method of almond protein in the prior art, a kind of recovery rate and the rate of deposition method that higher polyethylene glycol-Microwave-assisted Extraction is got almond protein is provided.
The technical scheme that solves the problems of the technologies described above is that the method that polyethylene glycol-Microwave-assisted Extraction is got almond protein comprises the steps:
(1) the defatted apricot kernel powder is crossed 60～80 mesh sieves, add volume fraction and be 25% polyglycol solution and be mixed with the mixed liquor that solid-liquid ratio is 1:18～30g/mL, 40～50 ℃ of power are 110～130w Microwave Extraction 3～6 minutes, under 8000～10000 rev/mins the condition, centrifugal 8～10 minutes, get supernatant;
(2) in the supernatant of step (1), add ethanol, the volume ratio of supernatant and ethanol is 1:0.2～0.3, stir and evenly mix, with the salt acid for adjusting pH value to 4 of 1.0mol/L～4.5, left standstill precipitation 3～5 minutes, centrifugal 5～8 minutes, abandoning supernatant is 4～4.5 water washing precipitate 2～4 times with the pH value, gets the almond protein precipitation;
(3) almond protein is deposited in-50～-60 ℃ of lower power and is 1.2～1.8KW, vacuum and be in the vacuum tank of 0.06～0.09MPa vacuum freeze drying 20～24 hours, and got natural almond protein powder.
Above-mentioned steps is crossed 60～80 mesh sieves with the defatted apricot kernel powder in (1), and adding volume fraction and be 25% polyglycol solution, to be mixed with solid-liquid ratio be that the mixed liquor of 1:20～25g/mL is good.
The better volume ratio of supernatant and ethanol is 1:0.24～0.27 in the above-mentioned steps (2).
After adding ethanol in the supernatant in the above-mentioned steps (2), the salt acid for adjusting pH value to 4.2 of usefulness 1.0mol/L～4.5 are good.
The dessert almond powder that above-mentioned defatted apricot kernel powder can be degreasing also can be the semen armeniacae amarae powder of degreasing.
Above-mentioned polyethylene glycol is Macrogol 200 (PEG-200), PEG400 (PEG-400) or Macrogol 600 (PEG-600), produce by close europeanized reagent Co., Ltd of Tianjin section, wherein the mean molecule quantity of Macrogol 200 is 190～210, the mean molecule quantity of PEG400 is 380～420, and the mean molecule quantity of Macrogol 600 is 560～640.
The almond powder of the method that polyethylene glycol-Microwave-assisted Extraction of the present invention is got almond protein after the degreasing is as raw material, get with polyethylene glycol-Microwave-assisted Extraction, isoelectric point-ethanol co precipitation and vacuum freeze-drying method extract almond protein, polyethylene glycol is that novel green solvent has solubilization to the almond protein effective ingredient, can improve and leach usefulness and recovery rate, and avirulence, Microwave-assisted Extraction heat-obtaining efficient is high, the intensification Quick uniform, greatly improved the extraction efficiency of almond protein, shortened extraction time, the purity of almond protein is higher in addition, and rate of deposition is high, and extracting method is simple, extraction time is shorter, and is environmentally friendly.
Description of drawings
Fig. 1 is that different solvents is to the impact effect figure of almond protein recovery rate.
The specific embodiment
Now the present invention is further specified, but the present invention is not limited only to following embodiment.
Embodiment 1
Take 100g degreasing dessert almond powder as raw material, the method that polyethylene glycol-Microwave-assisted Extraction is got almond protein comprises the steps:
(1) 100g degreasing dessert almond powder is crossed 70 mesh sieves, adding the 2200mL volume fraction and be 25% Macrogol 200 solution preparation, to become solid-liquid ratio be the mixed liquor of 1:22g/mL, 45 ℃ of power are 120w Microwave Extraction 4 minutes, under 9000 rev/mins condition, centrifugal 9 minutes, get supernatant, recovery rate is 93.35%.
(2) 550mL ethanol will be added in the supernatant of step (1), the volume ratio of supernatant and ethanol is 1:0.25, stir and evenly mix, salt acid for adjusting pH value with 1.0mol/L reaches 4.5, leaves standstill to make albumen precipitation in 4 minutes, centrifugal 6 minutes, abandon supernatant, be 4.5 water washing precipitate 3 times with the pH value, get the almond protein precipitation, rate of deposition is 98.81%.
(3) almond protein is deposited in-55 ℃ of power and is 1.5KW, vacuum and be in the vacuum tank of 0.08MPa vacuum freeze drying 22 hours, and got 41.50g crude sweet almond protein powder, according to formula
Embodiment 2
Take 100g degreasing dessert almond powder as raw material, the method that polyethylene glycol-Microwave-assisted Extraction is got almond protein comprises the steps:
(1) 100g degreasing dessert almond powder is crossed 70 mesh sieves, adding the 2000mL volume fraction and be 25% Macrogol 200 solution preparation, to become solid-liquid ratio be the mixed liquor of 1:20g/mL, 45 ℃ of power are 120w Microwave Extraction 4 minutes, under 9000 rev/mins the condition, centrifugal 9 minutes, get supernatant, recovery rate is 91.65%.
(2) 480mL ethanol will be added in the supernatant of step (1), the volume ratio of supernatant and ethanol is 1:0.24, stir and evenly mix, salt acid for adjusting pH value with 1.0mol/L reaches 4.5, leaves standstill 4 minutes precipitations, centrifugal 6 minutes, abandoning supernatant, be 4.5 water washing and precipitating thing 3 times with the pH value, get the almond protein precipitation, rate of deposition is 97.32%.
Other step is identical with embodiment 1, gets 40.14g crude sweet almond protein powder, and its purity of protein is 91.92%.
Embodiment 3
Take 100g degreasing dessert almond powder as raw material, the method that polyethylene glycol-Microwave-assisted Extraction is got almond protein comprises the steps:
(1) 100g degreasing dessert almond powder is crossed 70 mesh sieves, adding the 2500mL volume fraction and be 25% Macrogol 200 solution preparation, to become solid-liquid ratio be the mixed liquor of 1:25g/mL, 45 ℃ of power are 120w Microwave Extraction 4 minutes, under 9000 rev/mins the condition, centrifugal 9 minutes, get supernatant, recovery rate is 92.14%.
(2) 675mL ethanol will be added in the supernatant of step (1), the volume ratio of supernatant and ethanol is 1:0.27, stir and evenly mix, salt acid for adjusting pH value with 1.0mol/L reaches 4.5, leaves standstill 4 minutes precipitations, centrifugal 6 minutes, abandoning supernatant, be 4.5 water washing precipitate 3 times with the pH value, get the almond protein precipitation, rate of deposition is 96.78%.
Other step is identical with embodiment 1, gets 40.13g crude sweet almond protein powder, and its purity of protein is 92.53%.
Embodiment 4
Take 100g degreasing dessert almond powder as raw material, the method that polyethylene glycol-Microwave-assisted Extraction is got almond protein comprises the steps:
(1) 100g degreasing dessert almond powder is crossed 70 mesh sieves, adding the 1800mL volume fraction and be 25% Macrogol 200 solution preparation, to become solid-liquid ratio be the mixed liquor of 1:18g/mL, 45 ℃ of power are 120w Microwave Extraction 4 minutes, under 9000 rev/mins the condition, centrifugal 9 minutes, get supernatant, recovery rate is 90.26%.
(2) 360mL ethanol will be added in the supernatant of step (1), the volume ratio of supernatant and ethanol is 1:0.2, stir and evenly mix, salt acid for adjusting pH value with 1.0mol/L reaches 4.5, leaves standstill 4 minutes precipitations, centrifugal 6 minutes, abandoning supernatant, be 4.5 water washing precipitate 3 times with the pH value, get the almond protein precipitation, rate of deposition is 93.31%.
Other step is identical with embodiment 1, gets 37.90g crude sweet almond protein powder, and its purity of protein is 90.89%.
Embodiment 5
Take 100g degreasing dessert almond powder as raw material, the method that polyethylene glycol-Microwave-assisted Extraction is got almond protein comprises the steps:
(1) 100g degreasing dessert almond powder is crossed 70 mesh sieves, adding the 3000mL volume fraction and be 25% Macrogol 200 solution preparation, to become solid-liquid ratio be the mixed liquor of 1:30g/mL, 45 ℃ of power 120w Microwave Extraction 4 minutes, under 9000 rev/mins condition, centrifugal 9 minutes, get supernatant, recovery rate is 91.86%.
(2) 900mL ethanol will be added in the supernatant of step (1), the volume ratio of supernatant and ethanol is 1:0.3, stir and evenly mix, salt acid for adjusting pH value with 1.0mol/L reaches 4.5, leaves standstill 4 minutes precipitations, centrifugal 6 minutes, abandoning supernatant, be 4.5 water washing precipitate 3 times with the pH value, get the almond protein precipitation, rate of deposition is 95.24%.
Other step is identical with embodiment 1, gets 39.37g crude sweet almond protein powder, and its purity of protein is 90.28%.
Embodiment 6
Polyethylene glycol-the Microwave-assisted Extraction of above-described embodiment 1～5 is got in the method for almond protein, add volume fraction after in step (1), degreasing dessert almond powder being crossed 60 mesh sieves and be 25% polyglycol solution, 40 ℃ of power 110w Microwave Extraction 6 minutes, under 8000 rev/mins the condition, centrifugal 10 minutes, get supernatant, recovery rate is 92.84%; Be to add ethanol in the supernatant with step (1) in step (2), stir and evenly mix, reach 4 with the salt acid for adjusting pH value of 1.0mol/L, leave standstill 3 minutes precipitations, centrifugal 5 minutes, abandoning supernatant, be 4 water washing precipitate 2 times with the pH value, get the almond protein precipitation, rate of deposition is 98.81%; In step (3), be that 1.2KW, vacuum are vacuum freeze drying 24 hours in the vacuum tank of 0.06MPa with sediment at-50 ℃ of power.Other step is identical with corresponding embodiment, gets crude sweet almond protein powder.
Embodiment 7
Polyethylene glycol-the Microwave-assisted Extraction of above-described embodiment 1～5 is got in the method for almond protein, add volume fraction after in step (1), degreasing dessert almond powder being crossed 80 mesh sieves and be 25% polyglycol solution, at microwave power 130w, under the condition of temperature 50 C, Microwave Extraction 3 minutes, under 10000 rev/mins condition, centrifugal 8 minutes, get supernatant, recovery rate is 91.42%; To add ethanol in the supernatant with step (1) in step (2), stir and evenly mix, the pH value of regulating mixed liquor with the hydrochloric acid of 1.0mol/L reaches 4.2, leave standstill 5 minutes precipitations, centrifugal 8 minutes, abandoning supernatant was 4.2 water washing precipitate 4 times with the pH value, get the almond protein precipitation, rate of deposition is 98.81%; In step (3), be that 1.8KW, vacuum are vacuum freeze drying 20 hours in the vacuum tank of 0.09MPa with sediment at-60 ℃ of power.Other step is identical with corresponding embodiment, gets crude sweet almond protein powder.
Embodiment 8
Get in the method for almond protein at the polyethylene glycol-Microwave-assisted Extraction of above-described embodiment 1～7, the degreasing semen armeniacae amarae powder of quality such as use to replace in the degreasing dessert almond powder in the step 1, other step is with embodiment is identical accordingly.
Embodiment 9
Get in the method for almond protein at the polyethylene glycol-Microwave-assisted Extraction of above-described embodiment 1～8, the Macrogol 200 in the step 1 is replaced with the isopyknic PEG400 of isoconcentration or Macrogol 600, other step is identical with corresponding embodiment.
In order to determine the optimum condition of extracting method of the present invention, the inventor has carried out the lot of experiments screening, and concrete condition is as follows:
1, the polyethylene glycol solvent chooses
(1) alkaline extraction: the method mainly is to utilize the NaOH strong base solution that protein is extracted, accurately take by weighing first a certain amount of degreasing dessert almond powder, amount take liquid ratio as 1:22g/ml is 9.0 with the NaOH adjusting pH value of 0.5mol/L, extracted 4 minutes at microwave power 120W, temperature 45 C, get its supernatant, regulate pH value to 4.5 with 1.0mol/L HCl, albumen precipitation is got off, centrifugation, freeze drying get final product.
(2) salt formulation: the method mainly is to utilize NaCl solution that protein is extracted, accurately claim first a certain amount of degreasing dessert almond powder, NaCl with 0.15mol/L dissolves with the amount of liquid ratio 1:22g/ml, extracted 4 minutes at microwave power 120W, temperature 45 C, get its supernatant, regulate pH value to 4.5 with 1.0mol/L HCl, albumen precipitation is got off, centrifugation, freeze drying get final product.
(3) extraction method of the present invention: take by weighing a certain amount of degreasing dessert almond powder, be that 25% Macrogol 200 solution, PEG400 solution, Macrogol 600 solution dissolve take solid-liquid ratio as 1:22g/mL with volume fraction respectively, extracted 4 minutes at microwave power 120W, temperature 45 C, get its supernatant, regulate pH value to 4.5 with 1.0mol/L HCl, albumen precipitation gets off, and centrifugation, freeze drying get final product.
Calculate the dessert almond protein extracting ratio of above-mentioned 3 experiments according to formula,
The results are shown in Figure 1.As shown in Figure 1, under equal conditions, recovery rate when selected Macrogol 200 (PEG-200) of the present invention, PEG400 (PEG-400), Macrogol 600 (PEG-600) are solvent to the recovery rate of degreasing dessert almond albumen apparently higher than NaOH and the NaCl recovery rate, particularly Macrogol 200 during as solvent as solvent is the highest.The Macrogol 200 of comparing with PEG400, Macrogol 600 is more suitable for helping to improve the utilization rate of dessert almond albumen in the extraction of dessert almond albumen.
2, microwave handling method is chosen
(1) polyethylene glycol-ultrasonic assisted extraction: extracting degreasing dessert almond powder and volume fraction are 25% Macrogol 200 aqueous solution, solid-liquid ratio 1:22g/mL, under the power of 360W, temperature 45 C, extracted 20 minutes, extract is centrifugal 10 minutes at 8000 rev/mins, collect supernatant, with the content of protein in the Coomassie brilliant blue method mensuration supernatant, with the content of Kjeldahl nitrogen determination defatted apricot kernel powder total protein, calculate the recovery rate of dessert almond albumen, see Table 1;
(2) polyethylene glycol-conventional heating water bath extracts: degreasing dessert almond powder and the volume fraction of getting with the quality such as above-mentioned are 25% Macrogol 200 aqueous solution, solid-liquid ratio 1:22g/mL, under temperature 45 C, extracted 60 minutes, extract high speed centrifugation 10 minutes under 8000 rev/mins rotating speed, collect supernatant, with the content of protein in the Coomassie brilliant blue method mensuration supernatant, in like manner calculate the recovery rate of dessert almond albumen, the results are shown in Table 1;
(3) polyethylene glycol-Microwave-assisted firing extracts: getting with the degreasing dessert almond powder of the quality such as above-mentioned and volume fraction is 25% Macrogol 200 aqueous solution, solid-liquid ratio 1:22g/mL, Microwave Extraction is 4 minutes under temperature 45 C, microwave power is 120w, extract high speed centrifugation 10 minutes under 8000 rev/mins rotating speed is collected supernatant, measures the content of protein in the supernatant with the Coomassie brilliant blue method, in like manner calculate the recovery rate of dessert almond albumen, the results are shown in Table 1;
The comparison of table 1 Different Extraction Method
| Project | Time (minute) | Temperature (℃) | Recovery rate (%) |
| Polyethylene glycol-Microwave-assisted Extraction is got | 4 | 45 | 93.35 |
| Polyethylene glycol-ultrasonic assisted extraction | 20 | 45 | 84.36 |
| Polyethylene glycol-conventional heating water bath extracts | 60 | 45 | 78.61 |
Shown that by above-mentioned table 1 comparing result it is the highest that polyethylene glycol-Microwave-assisted Extraction is got extraction efficiency, extraction time is the shortest.Follow the example of with ultrasonic assisted Extraction and to compare, Microwave-assisted Extraction is got required time and has been reduced by 16 minutes, and almond protein recovery rate under optimum extraction condition nearly improves 10%; Compare with common water-bath heating extraction, Microwave-assisted Extraction is got required time and was reduced by 56 minutes, and the recovery rate of almond protein has nearly improved 15%.
3, the selection of intermediate processing
(1) isoelectric precipitation: with the almond protein supernatant in the embodiment of the invention 1, the pH value of regulating mixed liquor with the hydrochloric acid of 1.0mol/L reaches 4.5, leaves standstill postprecipitation, water washing and precipitating, and freeze drying is according to formula
Calculate the almond protein rate of deposition, the results are shown in Table 2.
(2) ethanol precipitation: with the almond protein supernatant in the embodiment of the invention 1, add the long-pending ethanol of tetraploid, stir, leave standstill postprecipitation, water washing and precipitating, freeze drying in like manner calculates almond protein rate of deposition (seeing Table 2).
(3) isoelectric point-ethanol co precipitation: with the almond protein supernatant of the embodiment of the invention 1, the ethanol that adds 0.25 times of volume, stir, salt acid for adjusting pH value with 1.0mol/L reaches 4.5, staticly settle, water washing and precipitating, almond protein rate of deposition (seeing Table 2) is in like manner calculated in freeze drying.
The different intermediate processing contrasts of table 2
| Isoelectric point precipitation | Ethanol precipitation | Isoelectric point-ethanol co precipitation method | |
| The precipitation required time (minute) | 3～5 | Leave standstill 180～240 | 3～5 |
| Rate of deposition (%) | 78.75 | 97.56 | 98.81 |
The comparing result of above-mentioned table 2 shows that isoelectric point-ethanol of the present invention is worked in coordination with method, and rate of deposition is the highest, can reach 98.81%, and required time consumption short, ethanol is few; The Ethanol Method rate of deposition is 97.56%, and near isoelectric point-ethanol synergy, but required time is long, and the ethanol consumption is many; The isoelectric point method rate of deposition only is 78.75%.This shows that isoelectric point-ethanol is worked in coordination with method, combine the fast and alcohol deposition method of isoelectric point method settling velocity and precipitate completely advantage, its rate of deposition height.
Claims (5)
1. the method that polyethylene glycol-Microwave-assisted Extraction is got almond protein is characterized in that comprising the steps:
(1) the defatted apricot kernel powder is crossed 60～80 mesh sieves, add volume fraction and be 25% polyglycol solution and be mixed with the mixed liquor that solid-liquid ratio is 1:18～30g/mL, 40～50 ℃ of power are 110～130w Microwave Extraction 3～6 minutes, under 8000～10000 rev/mins the condition, centrifugal 8～10 minutes, get supernatant;
(2) in the supernatant of step (1), add ethanol, the volume ratio of supernatant and ethanol is 1:0.2～0.3, stir and evenly mix, with the salt acid for adjusting pH value to 4 of 1.0mol/L～4.5, left standstill precipitation 3～5 minutes, centrifugal 5～8 minutes, abandoning supernatant is 4～4.5 water washing precipitate 2～4 times with the pH value, gets the almond protein precipitation;
(3) almond protein is deposited in-50～-60 ℃ of lower power and is 1.2～1.8KW, vacuum and be in the vacuum tank of 0.06～0.09MPa vacuum freeze drying 20～24 hours, and got natural almond protein powder.
2. polyethylene glycol-Microwave-assisted Extraction according to claim 1 is got the method for almond protein, it is characterized in that: described step is crossed 60～80 mesh sieves with the defatted apricot kernel powder in (1), adds volume fraction and is 25% polyglycol solution and be mixed with the mixed liquor that solid-liquid ratio is 1:20～25g/mL.
3. polyethylene glycol-Microwave-assisted Extraction according to claim 1 is got the method for almond protein, it is characterized in that: the volume ratio of supernatant and ethanol is 1:0.24～0.27 in the described step (2).
4. according to claim 1 or 3 described polyethylene glycol-Microwave-assisted Extractions method of getting almond protein, it is characterized in that: after adding ethanol in the supernatant in the described step (2), with the salt acid for adjusting pH value to 4.2 of 1.0mol/L～4.5.
5. polyethylene glycol-Microwave-assisted Extraction according to claim 1 and 2 is got the method for almond protein, it is characterized in that: the dessert almond powder that described defatted apricot kernel powder is degreasing or the semen armeniacae amarae powder of degreasing.
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| CN103919879A (en) * | 2014-04-17 | 2014-07-16 | 陕西师范大学 | Preparation method of health products containing procyanidin and chlorogenic acid from apricot kernel skin |
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| CN106173943A (en) * | 2016-07-08 | 2016-12-07 | 国家林业局泡桐研究开发中心 | A kind of Prunus armeniaca L.var.ansu Maxim. dried eucommia bark noodles and preparation method thereof |
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| CN107686856A (en) * | 2017-09-27 | 2018-02-13 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of preparation method of Pitaya Flower polypeptide |
| CN107686856B (en) * | 2017-09-27 | 2021-04-27 | 广西壮族自治区农业科学院农产品加工研究所 | Preparation method of pitaya flower protein polypeptide |
| CN107435060A (en) * | 2017-09-29 | 2017-12-05 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of preparation method of longan nuclear protein polypeptide |
| CN107647412A (en) * | 2017-09-29 | 2018-02-02 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of preparation method of longan nuclear protein polypeptide chewable tablets |
| CN107435060B (en) * | 2017-09-29 | 2021-02-09 | 广西壮族自治区农业科学院农产品加工研究所 | Preparation method of longan nucleoprotein polypeptide |
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