CN102839205A - Method for preparing oligopeptide epidermis cell growth factor dry powder - Google Patents
Method for preparing oligopeptide epidermis cell growth factor dry powder Download PDFInfo
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- CN102839205A CN102839205A CN2012102980096A CN201210298009A CN102839205A CN 102839205 A CN102839205 A CN 102839205A CN 2012102980096 A CN2012102980096 A CN 2012102980096A CN 201210298009 A CN201210298009 A CN 201210298009A CN 102839205 A CN102839205 A CN 102839205A
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- cell growth
- growth factor
- dry powder
- oligopeptides
- epithelical cell
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Abstract
A method for preparing oligopeptide epidermis cell growth factor dry powder includes a step of mixing proteases of different activities with part of additives according to a ratio; a step of putting mixed composite proteases into a polypeptide epidermis cell growth factor solution according to an actually required ratio and stirring the solution until the mixed composite proteases are dissolved well; a step of putting the mixed liquid into a liquid container, putting the liquid container into warm water, stirring the mixed liquid slightly, taking out the liquid container and putting the liquid container at room temperature after 20 minutes; a step of putting the liquid container into a vacuum extraction device for drying and extraction of the mixed liquid; and a step of putting the dry powder obtained from extraction into a bottle, sealing the bottle and preserving the dry powder at low temperature for later use. By means of the method, the molecular weight of obtained oligopeptide epidermis cell growth factors is far smaller than gaps of a skin cuticle, the oligopeptide epidermis cell growth factors are enabled to penetrate the gaps of the skin cuticle, enter the skin and play a good role in skin protection, and therefore the method for preparing the oligopeptide epidermis cell growth factor dry powder has good application prospects in the field of skincare and skin protection.
Description
Technical field
The present invention relates to the beauty and skin care field, specifically relate to a kind of method of making oligopeptides epithelical cell growth factor dry powder.
Background technology
Epithelical cell growth factor is the intravital a kind of protein and peptide material of people, and its maximum characteristics are to impel the propagation and the differentiation of epidermic cell, thereby replaces old and feeble with dead cell with new life's cell.The late nineteen eighties; Along with engineered development; People utilize the fast characteristics of growth and breeding such as intestinal bacteria and yeast to carry out industrial fermentation production, and use recombinant gene to produce this proteinoid peptide material identical with the human epidermal ESC.All the time, although epithelical cell growth factor effect aspect the healing of various skin wounds and operation is remarkable, practical function is limited in the beauty and skin care field.Trace it to its cause, still because the molecular weight of epithelical cell growth factor excessive (60KD～150KD), can't smooth transdermal stratum corneum gap (below the 10KD) due to.
Summary of the invention
The objective of the invention is to above-mentioned existing problems and deficiency; Provide a kind of manufacture craft simple, can easily the polypeptide epithelical cell growth factor be made into the method for the manufacturing oligopeptides epithelical cell growth factor dry powder of the oligopeptides epithelical cell growth factor that can see through the skin keratin lamellar spacing smoothly.
Technical scheme of the present invention is achieved in that
The method of manufacturing oligopeptides epithelical cell growth factor dry powder of the present invention is characterized in may further comprise the steps:
A, the proteolytic enzyme of different vigor is mixed with portions additive according to a certain percentage, then with the compound protease that mixes according to actual needs ratio put into and be stirred to dissolving fully in the polypeptide epithelical cell growth factor solution;
B, will dissolve mixed liquid and put in the liquid container and liquid container is placed 40 ℃～45 ℃ warm water, stir mixing liquid gently, and take out liquid container after 20 minutes and place room temperature;
C, liquid container put into the drying extraction of carrying out mixing liquid in vacuum freezing extraction plant or the vacuum microwave extraction plant;
D, will extract the dry powder that obtains after accomplishing and place sealing and for use certainly at low temperatures in the bottle, promptly accomplish the manufacturing of oligopeptides epithelical cell growth factor dry powder.
Wherein, the proteolytic enzyme of above-mentioned different vigor comprises papoid, bromeline.
Above-mentioned additive comprises dextrose anhydrous.
The ratio of above-mentioned compound protease that mixes and epithelical cell growth factor solution is 1:3000.
The proteolytic enzyme of above-mentioned different vigor and the ratio of additive are 1:10.
The molecular weight of above-mentioned oligopeptides epithelical cell growth factor dry powder is 0.5KD～3KD.
The invention has the beneficial effects as follows: through method of the present invention; Can easily the polypeptide epithelical cell growth factor be made into the oligopeptides epithelical cell growth factor; And the molecular weight of made oligopeptides epithelical cell growth factor is much smaller than the stratum corneum gap of skin; Thereby it can successfully transdermal stratum corneum gap be entered in the skin and play good skin care effect; Therefore it has a good application prospect in the beauty and skin care field, and manufacture craft of the present invention is simple, easy to operate, practical.
Embodiment
The method of manufacturing oligopeptides epithelical cell growth factor dry powder of the present invention may further comprise the steps:
At first; With the mixed that additives such as the proteolytic enzyme of these two kinds of different vigor of papoid and bromeline and dextrose anhydrous are pressed 1:10, the compound protease that mixes is put in the ratio of 1:3000 according to actual needs be stirred to dissolving fully in the polypeptide epithelical cell growth factor solution then;
Then, the mixed liquid of dissolving is put in the liquid container and with liquid container and placed 40 ℃～45 ℃ warm water, stir mixing liquid gently, take out liquid container after 20 minutes and place room temperature;
Then, put liquid container into carry out mixing liquid in vacuum freezing extraction plant or the vacuum microwave extraction plant drying extraction;
At last, the dry powder that obtains after the extraction completion is placed sealing and for use certainly at low temperatures in the bottle, promptly accomplish the manufacturing of oligopeptides epithelical cell growth factor dry powder, and the molecular weight of resulting oligopeptides epithelical cell growth factor dry powder is 0.5KD～3KD.
Through method of the present invention; Can easily the polypeptide epithelical cell growth factor be made into the oligopeptides epithelical cell growth factor; And since the molecular weight of made oligopeptides epithelical cell growth factor much smaller than the stratum corneum gap of skin; Thereby it can successfully transdermal stratum corneum gap be entered in the skin and play good skin care effect; Therefore it has a good application prospect in the beauty and skin care field, and manufacture craft of the present invention is simple, easy to operate, practical.
The present invention describes through embodiment; But the present invention is not constituted restriction, with reference to description of the invention, other variations of the disclosed embodiments; Professional person as for this area expects that easily such variation should belong within the scope thereof of the present invention.
Claims (6)
1. method of making oligopeptides epithelical cell growth factor dry powder is characterized in that may further comprise the steps:
A, the proteolytic enzyme of different vigor is mixed with portions additive according to a certain percentage, then with the compound protease that mixes according to actual needs ratio put into and be stirred to dissolving fully in the polypeptide epithelical cell growth factor solution;
B, will dissolve mixed liquid and put in the liquid container and liquid container is placed 40 ℃～45 ℃ warm water, stir mixing liquid gently, and take out liquid container after 20 minutes and place room temperature;
C, liquid container put into the drying extraction of carrying out mixing liquid in vacuum freezing extraction plant or the vacuum microwave extraction plant;
D, will extract the dry powder that obtains after accomplishing and place sealing and for use certainly at low temperatures in the bottle, promptly accomplish the manufacturing of oligopeptides epithelical cell growth factor dry powder.
2. according to the method for the said manufacturing oligopeptides of claim 1 epithelical cell growth factor dry powder, it is characterized in that the proteolytic enzyme of above-mentioned different vigor comprises papoid, bromeline.
3. according to the method for the said manufacturing oligopeptides of claim 1 epithelical cell growth factor dry powder, it is characterized in that above-mentioned additive comprises dextrose anhydrous.
4. according to the method for the said manufacturing oligopeptides of claim 1 epithelical cell growth factor dry powder, the ratio that it is characterized in that above-mentioned compound protease that mixes and epithelical cell growth factor solution is 1:3000.
5. according to the method for the said manufacturing oligopeptides of claim 1 epithelical cell growth factor dry powder, it is characterized in that the proteolytic enzyme of above-mentioned different vigor and the ratio of additive are 1:10.
6. according to the method for the said manufacturing oligopeptides of claim 1 epithelical cell growth factor dry powder, the molecular weight that it is characterized in that above-mentioned oligopeptides epithelical cell growth factor dry powder is 0.5KD～3KD.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102980096A CN102839205A (en) | 2012-08-21 | 2012-08-21 | Method for preparing oligopeptide epidermis cell growth factor dry powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102980096A CN102839205A (en) | 2012-08-21 | 2012-08-21 | Method for preparing oligopeptide epidermis cell growth factor dry powder |
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| Publication Number | Publication Date |
|---|---|
| CN102839205A true CN102839205A (en) | 2012-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012102980096A Pending CN102839205A (en) | 2012-08-21 | 2012-08-21 | Method for preparing oligopeptide epidermis cell growth factor dry powder |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106727246A (en) * | 2017-02-08 | 2017-05-31 | 上海元茵生物科技有限公司 | Calf serum cell nutrient solution |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4743679A (en) * | 1986-02-24 | 1988-05-10 | Creative Biomolecules, Inc. | Process for producing human epidermal growth factor and analogs thereof |
-
2012
- 2012-08-21 CN CN2012102980096A patent/CN102839205A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4743679A (en) * | 1986-02-24 | 1988-05-10 | Creative Biomolecules, Inc. | Process for producing human epidermal growth factor and analogs thereof |
Non-Patent Citations (2)
| Title |
|---|
| 姜国辉等: "重组人表皮生长因子透皮吸收实验研究", 《中国药学杂志》 * |
| 陈秋东: "中等纯度重组人表皮生长因子（rhEGF）的分离纯化及在美容产品中的应用", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106727246A (en) * | 2017-02-08 | 2017-05-31 | 上海元茵生物科技有限公司 | Calf serum cell nutrient solution |
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Application publication date: 20121226 |