CN102835314A - Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting - Google Patents
Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting Download PDFInfo
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- CN102835314A CN102835314A CN2012103496052A CN201210349605A CN102835314A CN 102835314 A CN102835314 A CN 102835314A CN 2012103496052 A CN2012103496052 A CN 2012103496052A CN 201210349605 A CN201210349605 A CN 201210349605A CN 102835314 A CN102835314 A CN 102835314A
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- photinia glabra
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Abstract
The invention discloses a photinia serrulata tissue culture seedling rooting culture medium and a culture method for the photinia serrulata tissue culture seedling rooting. The photinia serrulata tissue culture seedling rooting culture medium provided by the invention is characterized in that on the basis of an MS (Murashige and Skoog) culture medium, the types and the concentration of major elements are regulated, the hormone regulation and control, the agar concentration and the like are optimized, the balanced and reasonable nutrition requirements are provided for the photinia serrulata tissue culture rooting seedlings, the fast, efficient and healthy growth of the photinia serrulata tissue culture rooting seedlings are ensured, and the generation of callus and vitrification phenomena is effectively controlled.
Description
Technical field
The present invention relates to a kind of plant tissue culture root media, particularly photinia glabra tissue cultivating seedling root media and cultural method thereof.
Background technology
Photinia glabra (Photinia frasery Dress) is the crossbreed of rose family Photinia; Be evergreen dungarunga or racemosus bush; Because of the characteristics of its scarlet tender leaf and anti-pruning, become important color leaf ornamental tree species in recent years, aspect gardens, obtained important application.
Because photinia glabra is a crossbreed, large-scale sexual propagation receives very big restriction, and seedling quality difference ginseng is uneven, is difficult to satisfy the great demand in market.The modes of reproduction of photinia glabra comprises cottage propagation, tissue culture propagation, propagation by grafiting, seed propagation, and group training and cuttage are its main modess of reproduction.With respect to cuttage seeding, tissue cultivating seedling does not receive season limit, can keep parent's merit preferably, is more suitable in factorial seedling growth.
Whether tissue culture is successful, depends on the selection to medium to a great extent.At present, the medium that generally uses of Plant Tissue Breeding is the MS medium.It is by macroelement (potassium nitrate; Ammonium nitrate; Potassium dihydrogen phosphate; Magnesium sulfate and calcium chloride); Trace element (KI; Boric acid; Manganese sulphate; Zinc sulphate; Sodium molybdate; Copper sulphate and cobalt chloride); Molysite (disodium ethylene diamine tetraacetate and ferrous sulfate); Organic matter (inositol; Glycine; Thiamine hydrochloride; Puridoxine hydrochloride and nicotinic acid); Hormone (6-benzyl aminopurine and methyl); Sucrose and agar are formed: macroelement guarantees the mineral nutrition that plant strain growth is required and quickens callus Growth; Trace element, molysite and organic matter give the comprehensive nutrition of plant and guarantee, guarantee the plant normal growth; The regulation and control of hormone make plant healthy and strong fast growth under isolated condition; Sucrose is that plant strain growth provides essential carbon source and regulates osmotic pressure; Agar rises plant and supports and fixation.
The technical problem of photinia glabra group training factorial seedling growth does not also have solution fully; When adopting MS medium photinia glabra tissue cultivating seedling culture of rootage; The following problem of normal appearance: group is trained the seedling base portion callus of taking root and is expanded, and the number of taking root is few, and rooting rate is low; The root system vigor is low, repeatedly is prone to callus and vitrification phenomenon etc. behind the subculture.
Summary of the invention
In view of this,, the objective of the invention is to the MS medium is improved, a kind of root media and cultural method thereof of suitable photinia glabra tissue cultivating seedling is provided for overcoming the deficiency of prior art.
For reaching this purpose, photinia glabra tissue cultivating seedling root media of the present invention, component is as shown in the table:
Cultural method may further comprise the steps:
(1) culture of rootage: with the subculture robust growth in the bud of growing thickly; Leaf leaf look normal; The bud of the about 1.5～2cm of plant height cuts down to be transferred in the above-mentioned root media takes root, and cultivation temperature is 25 ± 1 ℃, and intensity of illumination is 2000～3000Lux; Light application time is 10 hours every days, obtains test-tube plantlet.
(2) test-tube seedling transplanting: it is to cultivate in perlite-peat soil mixed-matrix of 3: 7 that step (1) gained test-tube seedling transplanting is gone into volume ratio, promptly gets the photinia glabra tissue cultivating seedling.
Beneficial effect of the present invention is: photinia glabra root media of the present invention; On the basis of MS medium; Through the kind and the concentration of adjustment macroelement, optimize hormone regulating and controlling and agar concentration etc., for the photinia glabra group is trained the nutritional need that the seedling of taking root provides balanced and reasonable; Guarantee its quick, efficient, healthy and strong growth, effectively controlled the generation of callus and vitrification phenomenon.
Embodiment
Below in conjunction with specific embodiment, the present invention is elaborated.
1, experiment material
Photinia glabra group training subculture seedling with in flowers research institute of the Institute Of Unity and Coherence In Writing Of Chongqing group training chamber is an experiment material.
2, experimental technique
Confirm the basic components (seeing table 1) of photinia glabra root media according to preliminary experiment result and long-term practical experience, and with KNO in the basic components
3, NH
4NO
3, 4 kinds of compositions such as NAA, IBA concentration adjust respectively, and other component and concentration thereof remain unchanged (seeing table 2), observe taking root and growing state of photinia glabra group training subculture seedling under every kind of composition variable concentrations, to optimize the culture of rootage based formulas.KNO wherein
3And NH
4NO
3Mainly be the optimization of training the seedling growing state of taking root to group, NAA and IBA are primarily aimed at the optimization that group is trained the seedling rooting situation of taking root.
The basic components of table 1 photinia glabra tissue cultivating seedling root media
The experimental formula of table 2 photinia glabra root media
* the same basic components of all the other components and concentration thereof.
Choose the consistent and healthy and strong photinia glabra group training subculture seedling of growth; Be inoculated in above-mentioned prescription (in the root media of the M1 of table 2～M17); Every kind of prescription is inoculated 50 bottles; Repeat 3 times, each culture of rootage 20 days add up growing state (height of seedling, leaf, leaf look, healthy and strong degree) and the situation of taking root (callus, take root several, rooting rate) of the tissue cultivating seedling that every kind of prescription cultivates etc.
3, experimental result
1) KNO
3The influence of giving birth to photinia glabra group training subculture seedling rooting
KNO in the adjustment basic components
3Concentration, all the other components and concentration thereof 3 results that remain unchanged see table 3, along with KNO
3The increase of concentration, bud height of seedling degree and healthy and strong degree all demonstrate the trend that raises and reduce earlier, and the bud seedling growth of the M4 that wherein fills a prescription is the highest, and average height reaches 1.8cm and robust growth, continues to increase KNO
3Concentration, bud height of seedling degree reduces gradually and growing way dies down.In these 5 prescriptions, the bud seedling is leaf all normal, and does not all have callus and vitrification phenomenon generation.Take all factors into consideration, be fit to the KNO of photinia glabra group training subculture seedling rooting
3Optium concentration be 900mg/L.
Table 3KNO
3The photinia glabra group is trained the influence of the seedling growing state of taking root
2) NH
4NO
3The photinia glabra group is trained the influence of the seedling growing state of taking root
NH in the adjustment basic components
4NO
3Concentration, all the other components and concentration thereof remain unchanged.The result sees table 4, along with NH
4NO
3The increase of concentration, the leaf trend that raises and afterwards reduce earlier that demonstrates.In these five prescriptions, the growth of bud seedling is all healthy and strong, and the leaf look all normal, and wherein the height of seedling of M9 is slightly high.Take all factors into consideration, be fit to the NH that the photinia glabra tissue cultivating seedling is taken root
4NO
3Optium concentration be 900mg/L.
Table 4NH
4NO
3The photinia glabra group is trained the influence of the seedling growing state of taking root
3) NAA is to the take root influence of situation of photinia glabra tissue cultivating seedling
The NAA of variable concentrations is very big to the influence of taking root of photinia glabra group training subculture seedling, is mainly reflected on rooting rate and the bud seedling rooting position, and along with the rising of concentration, its rooting rate is high more, but its base portion callus also has the trend of increase.The present invention considers that from the comprehensive condition of rooting rate and bud seedling the optium concentration of the NAA that suitable photinia glabra tissue cultivating seedling is taken root is 0.2mg/L.
Table 5NAA is to the take root influence of situation of photinia glabra tissue cultivating seedling
4) IBA is to the take root influence of situation of photinia glabra tissue cultivating seedling
The IBA of variable concentrations is very big to the influence of taking root of photinia glabra group training subculture seedling, is mainly reflected on rooting rate and the bud seedling rooting position, and along with the rising of concentration, its rooting rate is high more, but its base portion callus also has the trend of increase.The present invention considers that from the comprehensive condition of rooting rate and bud seedling the optium concentration of the IBA that suitable photinia glabra tissue cultivating seedling is taken root is 0.2mg/L.
Table 6IBA is to the take root influence of situation of photinia glabra tissue cultivating seedling
5) photinia glabra group training subculture seedling rooting medium
Comprehensive above-mentioned experimental result, photinia glabra group training subculture seedling rooting medium as follows:
Should be understood that, concerning those of ordinary skills, can improve or conversion, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention according to above-mentioned explanation.
Claims (2)
1. photinia glabra tissue cultivating seedling root media, it is characterized in that: the raw material mixed preparing by following mass concentration forms:
2. the culture of rootage method that photinia glabra tissue cultivating seedling root media according to claim 1 carries out is characterized in that, may further comprise the steps:
(1) culture of rootage: with the subculture robust growth in the bud of growing thickly; Leaf leaf look normal; The bud of the about 1.5～2cm of plant height cuts down to be transferred in the said root media takes root, and cultivation temperature is 25 ± 1 ℃, and intensity of illumination is 2000～3000Lux; Light application time is 10 hours every days, obtains test-tube plantlet.
(2) test-tube seedling transplanting: it is to cultivate in perlite-peat soil mixed-matrix of 3: 7 that step (1) gained test-tube seedling transplanting is gone into volume ratio, promptly gets the photinia glabra tissue cultivating seedling.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548685A (en) * | 2013-11-01 | 2014-02-05 | 重庆文理学院 | Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method |
| CN106962205A (en) * | 2017-06-02 | 2017-07-21 | 合肥华盖生物科技有限公司 | A kind of photinia glabra tissue-cultured seedling root media and cultural method |
| CN115093276A (en) * | 2022-05-13 | 2022-09-23 | 和田博正农业科技有限公司 | Preparation and application method of broad-spectrum rooting agent |
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| CN103548685A (en) * | 2013-11-01 | 2014-02-05 | 重庆文理学院 | Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method |
| CN106962205A (en) * | 2017-06-02 | 2017-07-21 | 合肥华盖生物科技有限公司 | A kind of photinia glabra tissue-cultured seedling root media and cultural method |
| CN115093276A (en) * | 2022-05-13 | 2022-09-23 | 和田博正农业科技有限公司 | Preparation and application method of broad-spectrum rooting agent |
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