CN106962205A - A kind of photinia glabra tissue-cultured seedling root media and cultural method - Google Patents

A kind of photinia glabra tissue-cultured seedling root media and cultural method Download PDF

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Publication number
CN106962205A
CN106962205A CN201710406768.2A CN201710406768A CN106962205A CN 106962205 A CN106962205 A CN 106962205A CN 201710406768 A CN201710406768 A CN 201710406768A CN 106962205 A CN106962205 A CN 106962205A
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China
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root media
photinia glabra
cultured seedling
tissue
glabra tissue
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Chinese (zh)
Inventor
张安育
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Hefei Huagai Biotechnology Co Ltd
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Hefei Huagai Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants

Abstract

The invention belongs to plant tissue culture root media technical field, there is provided a kind of photinia glabra tissue-cultured seedling root media and cultural method, the photinia glabra tissue-cultured seedling root media is using MS culture mediums as minimal medium, the 19g/L of sucrose 10 is added in MS culture mediums, the 2.55g/L of activated carbon 1.5, the 1.7g/L of organic fertilizer component 1.5, sterilization powder 1.5 2.6ml/L, the 1.8g/L of mineral salt compositions 0.5, the pH value of the root media is 5.4 6.1.The present invention, by adjusting the species and concentration of a great number of elements, optimization hormone regulating and controlling etc., the nutritional need of balanced and reasonable is provided for photinia glabra tissue culture rooted seedling on the basis of MS culture mediums, promotes its quick, efficient, robust growth.

Description

A kind of photinia glabra tissue-cultured seedling root media and cultural method
Technical field
The invention belongs to plant tissue culture root media technical field, in particular it relates to which a kind of photinia glabra tissue-cultured seedling is given birth to Root culture medium and cultural method.
Background technology
Photinia glabra is the cenospecies of rose family Photinia, is evergreen dungarunga or racemosus bush, because its is scarlet The characteristics of tender leaf and resistance to pruning, turn into important color leaf ornamental tree species in recent years, important answer has been obtained in terms of gardens With.Because photinia glabra is cenospecies, large-scale generative propagation is extremely restricted, and seedling quality difference ginseng is uneven, it is difficult to Meet the great demand in market.
The modes of reproduction of photinia glabra includes cutting propagation, tissue culture propagation, propagation by grafiting, seed propagation, tissue culture and cuttage It is its main modes of reproduction.Relative to cuttage seeding, tissue-cultured seedling is not subject to seasonal restrictions, and can preferably keep the Optimality of parent Shape, more suitable for industrial seedling rearing.Whether tissue cultures succeed, and depend greatly on the selection to culture medium.
The technical problem of photinia glabra tissue culture industrial seedling rearing is not fully solved also, using MS culture medium photinia glabra tissue cultures During seedling rooting culture, often there is problems with:Tissue culture rooted seedling base portion callus expands, and number of taking root is few, and rooting rate is low, root system Vigor is low, easily callus and vitrification phenomenon etc. occurs after multiple subculture.
The content of the invention
A kind of photinia glabra tissue-cultured seedling root media provided for defect of the prior art, the present invention, in MS trainings Support on the basis of base, be photinia glabra tissue culture rooted seedling by adjusting the species and concentration of a great number of elements, optimization hormone regulating and controlling etc. There is provided the nutritional need of balanced and reasonable, promote its quick, efficient, robust growth.
A kind of photinia glabra tissue-cultured seedling root media provided according to an aspect of the present invention, the photinia glabra tissue-cultured seedling Root media adds sucrose 10-19g/L, activated carbon 1.5- using MS culture mediums as minimal medium in MS culture mediums 2.55g/L, organic fertilizer component 1.5-1.7g/L, sterilize powder 1.5-2.6ml/L, mineral salt compositions 0.5-1.8g/L, with text Fire heating, and be stirred continuously, said components are dissolved, 1mol/L hydrochloric acid regulation pH value is added, makes the root media PH value is added and is put into after 3-6g agar, stirring in pressurization autoclave, gone out under the conditions of 105-121 DEG C between 5.4-6.1 Bacterium 20-30min.
Preferably, the photinia glabra tissue-cultured seedling root media is using MS culture mediums as minimal medium, in MS culture mediums Middle addition sucrose 15-19g/L, activated carbon 1.7-2.55g/L, organic fertilizer component 1.6-1.7g/L, sterilization powder 1.9-2.6ml/ L, mineral salt compositions 0.5-1.1g/L, is heated with slow fire, and is stirred continuously, and dissolves said components, adds 1mol/L salt Acid regulation pH value, make the pH value of the root media between 5.4-6.0, add be put into after 3-4g agar, stirring plus Press in autoclave, sterilize 20-25min under the conditions of 105-121 DEG C.
Preferably, the photinia glabra tissue-cultured seedling root media is using MS culture mediums as minimal medium, in MS culture mediums Middle addition sucrose 17g/L, activated carbon 1.85g/L, organic fertilizer component 1.6g/L, sterilize powder 2.5ml/L, mineral salt compositions 0.9g/L, is heated with slow fire, and is stirred continuously, and dissolves said components, is added 1mol/L hydrochloric acid regulation pH value, is made the life The pH value of root culture medium is 5.6, adds and is put into after 3-4g agar, stirring in pressurization autoclave, under the conditions of 105-121 DEG C Sterilize 20min.
Preferably, the organic fertilizer component formula is specific as follows:Wheat bran 3000g, lactic acid bacteria 500g, corn steep liquor 500g, water 50000g, rice bran 3000g, saccharomycete 500g, phosphate fertilizer 500g, nitrogenous fertilizer 6000g, marine alga 3000g, crushed bone meal 500g, fish Bone meal 500g.
Preferably, the mineral salt compositions formula is specific as follows:Ammonium nitrate 0.46g/L, calcium chloride 0.17g/L, inositol 0.1g/L, potassium carbonate 2.83g/L, 1- methyl α-naphthyl acetate 2mg/L, sodium dihydrogen phosphate 0.4g/L, magnesium carbonate 0.19g/L.
Preferably, the sterilization powder formula is specific as follows:5-8 parts of honeysuckle, 5-8 parts of the tuber of stemona, 5-8 parts of dittany bark, rhizoma atractylodis 3-6 parts, 3-6 parts of wrinkled giant hyssop, 4-7 parts of frutus cnidii.
Preferably, the sterilization powder fineness is 60-90 mesh.
Preferably, the activated carbon is granularity 60-90 mesh.
On the one hand a kind of method using its fermented stalk provided, the method using its fermented stalk includes as follows Step:
Step(1):By robust growth in subculture Multiple Buds, leaf leaf color is normal, and plant height about 1.5-2cm bud cuts down switching Taken root into the photinia glabra tissue-cultured seedling root media, cultivation temperature is 24-29 DEG C, and intensity of illumination is 2000- 2800Lux, light application time is daily 8 hours, obtains test tube seedling;
Step(2):By step(1)Gained test tube seedling is transplanted into perlite-peat soil mixed-matrix that volume ratio is 4: 8 and trained Support, you can.
Compared with prior art, the present invention has following beneficial effect:
(1)A kind of photinia glabra tissue-cultured seedling root media that the present invention is provided, adds sucrose in MS culture mediums, activated carbon, Organic fertilizer component, sterilizes powder, mineral salt compositions, photinia glabra root media of the invention, on the basis of MS culture mediums On, by adjusting the species and concentration of inorganic salts, optimization hormone regulating and controlling etc., balanced conjunction is provided for photinia glabra tissue culture rooted seedling The nutritional need of reason, it is ensured that its quick, efficient, robust growth.
The photinia glabra tissue-cultured seedling root media of the present invention has good facilitation to root system development, makes photinia glabra Tissue-cultured seedling breeding is effectively improved, and then promotes the industrialization production of photinia glabra, is to cultivate high-quality red autumnal leaves on a large scale Chinese photinia seedling provides facility;
(2)Present invention sterilization powder formula is made up of honeysuckle, the tuber of stemona, dittany bark, rhizoma atractylodis, wrinkled giant hyssop, frutus cnidii, Chinese herbal medicine sterilization Agent is pure Chinese herbal medicine composition, and active component is pure chemistry composition, environmental suitability problem is not present after, also not by environment bar The influence of part, thus in Field information instant effect, effect is good, this point is better than each component in chemical series bactericidal agent, formula It is that, to plant and environmentally friendly composition in traditional Chinese medical science prescription, high safety, nontoxic residue-free promotes photinia glabra tissue-cultured seedling to be good for Kang Shengchang, reduces the incidence of pest and disease damage.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention Rather than limitation the scope of the present invention.
A kind of photinia glabra tissue-cultured seedling root media that the present invention is provided, on the basis of MS culture mediums, passes through adjustment The species and concentration of a great number of elements, optimization hormone regulating and controlling etc., the nutrition of balanced and reasonable is provided for photinia glabra tissue culture rooted seedling Demand, promotes its quick, efficient, robust growth.
A kind of photinia glabra tissue-cultured seedling root media provided according to an aspect of the present invention, the photinia glabra tissue-cultured seedling Root media adds sucrose 10-19g/L, activated carbon 1.5- using MS culture mediums as minimal medium in MS culture mediums 2.55g/L, organic fertilizer component 1.5-1.7g/L, sterilize powder 1.5-2.6ml/L, mineral salt compositions 0.5-1.8g/L, with text Fire heating, and be stirred continuously, said components are dissolved, 1mol/L hydrochloric acid regulation pH value is added, makes the root media PH value is added and is put into after 3-6g agar, stirring in pressurization autoclave, gone out under the conditions of 105-121 DEG C between 5.4-6.1 Bacterium 20-30min.
Preferably, the photinia glabra tissue-cultured seedling root media is using MS culture mediums as minimal medium, in MS culture mediums Middle addition sucrose 15-19g/L, activated carbon 1.7-2.55g/L, organic fertilizer component 1.6-1.7g/L, sterilization powder 1.9-2.6ml/ L, mineral salt compositions 0.5-1.1g/L, is heated with slow fire, and is stirred continuously, and dissolves said components, adds 1mol/L salt Acid regulation pH value, make the pH value of the root media between 5.4-6.0, add be put into after 3-4g agar, stirring plus Press in autoclave, sterilize 20-25min under the conditions of 105-121 DEG C.
Preferably, the photinia glabra tissue-cultured seedling root media is using MS culture mediums as minimal medium, in MS culture mediums Middle addition sucrose 17g/L, activated carbon 1.85g/L, organic fertilizer component 1.6g/L, sterilize powder 2.5ml/L, mineral salt compositions 0.9g/L, is heated with slow fire, and is stirred continuously, and dissolves said components, is added 1mol/L hydrochloric acid regulation pH value, is made the life The pH value of root culture medium is 5.6, adds and is put into after 3-4g agar, stirring in pressurization autoclave, under the conditions of 105-121 DEG C Sterilize 20min.
Preferably, the organic fertilizer component formula is specific as follows:Wheat bran 3000g, lactic acid bacteria 500g, corn steep liquor 500g, water 50000g, rice bran 3000g, saccharomycete 500g, phosphate fertilizer 500g, nitrogenous fertilizer 6000g, marine alga 3000g, crushed bone meal 500g, fish Bone meal 500g.
Preferably, the mineral salt compositions formula is specific as follows:Ammonium nitrate 0.46g/L, calcium chloride 0.17g/L, inositol 0.1g/L, potassium carbonate 2.83g/L, 1- methyl α-naphthyl acetate 2mg/L, sodium dihydrogen phosphate 0.4g/L, magnesium carbonate 0.19g/L.
Preferably, the sterilization powder formula is specific as follows:5-8 parts of honeysuckle, 5-8 parts of the tuber of stemona, 5-8 parts of dittany bark, rhizoma atractylodis 3-6 parts, 3-6 parts of wrinkled giant hyssop, 4-7 parts of frutus cnidii.
Preferably, the sterilization powder fineness is 60-90 mesh.
Preferably, the activated carbon is granularity 60-90 mesh.
On the one hand a kind of method using its fermented stalk provided, the method using its fermented stalk includes as follows Step:
Step(1):By robust growth in subculture Multiple Buds, leaf leaf color is normal, and plant height about 1.5-2cm bud cuts down switching Taken root into the photinia glabra tissue-cultured seedling root media, cultivation temperature is 24-29 DEG C, and intensity of illumination is 2000- 2800Lux, light application time is daily 8 hours, obtains test tube seedling;
Step(2):By step(1)Gained test tube seedling is transplanted into perlite-peat soil mixed-matrix that volume ratio is 4: 8 and trained Support, produce photinia glabra tissue-cultured seedling.
Compared with prior art, the present invention has following beneficial effect:
(1)A kind of photinia glabra tissue-cultured seedling root media that the present invention is provided, adds sucrose in MS culture mediums, activated carbon, Organic fertilizer component, sterilizes powder, mineral salt compositions, photinia glabra root media of the invention, on the basis of MS culture mediums On, by adjusting the species and concentration of inorganic salts, optimization hormone regulating and controlling etc., balanced conjunction is provided for photinia glabra tissue culture rooted seedling The nutritional need of reason, it is ensured that its quick, efficient, robust growth.
The photinia glabra tissue-cultured seedling root media of the present invention has good facilitation to root system, makes photinia glabra tissue culture Seedling breeding is effectively improved, and then promotes the industrialization production of photinia glabra, is to cultivate high-quality photinia glabra on a large scale Seedling provides facility;
(2)Present invention sterilization powder formula is made up of honeysuckle, the tuber of stemona, dittany bark, rhizoma atractylodis, wrinkled giant hyssop, frutus cnidii, Chinese herbal medicine sterilization Agent is pure Chinese herbal medicine composition, and active component is pure chemistry composition, environmental suitability problem is not present after, also not by environment bar The influence of part, thus in Field information instant effect, effect is good, this point is better than each component in chemical series bactericidal agent, formula It is that, to plant and environmentally friendly composition in traditional Chinese medical science prescription, high safety, nontoxic residue-free promotes photinia glabra tissue-cultured seedling to be good for Kang Shengchang, reduces the incidence of pest and disease damage.
Embodiment 1
A kind of photinia glabra tissue-cultured seedling root media that on the one hand the present embodiment provides, the photinia glabra tissue culture seedling rooting training Base is supported using MS culture mediums as minimal medium, sucrose 19g/L, activated carbon 1.5g/L, organic fertilizer group are added in MS culture mediums Divide 1.7g/L, sterilize powder 1.5ml/L, mineral salt compositions 1.8g/L, heated with slow fire, and be stirred continuously, make said components molten Solution, adds 1mol/L hydrochloric acid regulation pH value, makes the pH value of the root media between 5.4-6.1, add 3-6g's It is put into after agar, stirring in pressurization autoclave, sterilize 20-30min under the conditions of 121 DEG C.
The organic fertilizer component formula is specific as follows:Wheat bran 3000g, lactic acid bacteria 500g, corn steep liquor 500g, water 50000g, rice bran 3000g, saccharomycete 500g, phosphate fertilizer 500g, nitrogenous fertilizer 6000g, marine alga 3000g, crushed bone meal 500g, fishbone dust 500g。
The mineral salt compositions formula is specific as follows:Ammonium nitrate 0.46g/L, calcium chloride 0.17g/L, inositol 0.1g/L, Potassium carbonate 2.83g/L, 1- methyl α-naphthyl acetate 2mg/L, sodium dihydrogen phosphate 0.4g/L, magnesium carbonate 0.19g/L.
The sterilization powder formula is specific as follows:8 parts of honeysuckle, 5 parts of the tuber of stemona, 8 parts of dittany bark, 3 parts of rhizoma atractylodis, 6 parts of wrinkled giant hyssop, snake 4 parts of machine tool.
The sterilization powder fineness is 90 mesh.
The activated carbon is the mesh of granularity 90.
On the one hand a kind of method using its fermented stalk provided, the method using its fermented stalk includes as follows Step:
Step(1):By robust growth in subculture Multiple Buds, leaf leaf color is normal, and plant height about 1.5-2cm bud cuts down switching Taken root into the photinia glabra tissue-cultured seedling root media, cultivation temperature is 24-29 DEG C, and intensity of illumination is 2000- 2800Lux, light application time is daily 8 hours, obtains test tube seedling;
Step(2):By step(1)Gained test tube seedling is transplanted into perlite-peat soil mixed-matrix that volume ratio is 4: 8 and trained Support, produce photinia glabra tissue-cultured seedling.
Embodiment 2
A kind of photinia glabra tissue-cultured seedling root media that on the one hand the present embodiment provides, the photinia glabra tissue culture seedling rooting training Base is supported using MS culture mediums as minimal medium, sucrose 10g/L, activated carbon 2.55g/L, organic fertilizer group are added in MS culture mediums Divide 1.5g/L, sterilize powder 2.6ml/L, mineral salt compositions 0.5g/L, heated with slow fire, and be stirred continuously, make said components molten Solution, adds 1mol/L hydrochloric acid regulation pH value, makes the pH value of the root media between 5.4-6.1, add 3-6g's It is put into after agar, stirring in pressurization autoclave, sterilize 20-30min under the conditions of 121 DEG C.
The organic fertilizer component formula is specific as follows:Wheat bran 3000g, lactic acid bacteria 500g, corn steep liquor 500g, water 50000g, rice bran 3000g, saccharomycete 500g, phosphate fertilizer 500g, nitrogenous fertilizer 6000g, marine alga 3000g, crushed bone meal 500g, fishbone dust 500g。
The mineral salt compositions formula is specific as follows:Ammonium nitrate 0.46g/L, calcium chloride 0.17g/L, inositol 0.1g/L, Potassium carbonate 2.83g/L, 1- methyl α-naphthyl acetate 2mg/L, sodium dihydrogen phosphate 0.4g/L, magnesium carbonate 0.19g/L.
The sterilization powder formula is specific as follows:5 parts of honeysuckle, 8 parts of the tuber of stemona, 5 parts of dittany bark, 6 parts of rhizoma atractylodis, 3 parts of wrinkled giant hyssop, snake 7 parts of machine tool.
The sterilization powder fineness is 60 mesh.
The activated carbon is the mesh of granularity 60.
On the one hand a kind of method using its fermented stalk provided, the method using its fermented stalk includes as follows Step:
Step(1):By robust growth in subculture Multiple Buds, leaf leaf color is normal, and plant height about 1.5-2cm bud cuts down switching Taken root into the photinia glabra tissue-cultured seedling root media, cultivation temperature is 24-29 DEG C, and intensity of illumination is 2000- 2800Lux, light application time is daily 8 hours, obtains test tube seedling;
Step(2):By step(1)Gained test tube seedling is transplanted into perlite-peat soil mixed-matrix that volume ratio is 4: 8 and trained Support, produce photinia glabra tissue-cultured seedling.
Embodiment 3
A kind of photinia glabra tissue-cultured seedling root media that on the one hand the present embodiment provides, the photinia glabra tissue culture seedling rooting training Base is supported using MS culture mediums as minimal medium, sucrose 19g/L, activated carbon 1.7g/L, organic fertilizer group are added in MS culture mediums Divide 1.7g/L, sterilize powder 1.9ml/L, mineral salt compositions 1.1g/L, heated with slow fire, and be stirred continuously, make said components molten Solution, adds 1mol/L hydrochloric acid regulation pH value, makes the pH value of the root media between 5.4-6.0, add 3-4g's It is put into after agar, stirring in pressurization autoclave, sterilize 20-25min under the conditions of 105-121 DEG C.
The organic fertilizer component formula is specific as follows:Wheat bran 3000g, lactic acid bacteria 500g, corn steep liquor 500g, water 50000g, rice bran 3000g, saccharomycete 500g, phosphate fertilizer 500g, nitrogenous fertilizer 6000g, marine alga 3000g, crushed bone meal 500g, fishbone dust 500g。
The mineral salt compositions formula is specific as follows:Ammonium nitrate 0.46g/L, calcium chloride 0.17g/L, inositol 0.1g/L, Potassium carbonate 2.83g/L, 1- methyl α-naphthyl acetate 2mg/L, sodium dihydrogen phosphate 0.4g/L, magnesium carbonate 0.19g/L.
The sterilization powder formula is specific as follows:6 parts of honeysuckle, 6 parts of the tuber of stemona, 7 parts of dittany bark, 5 parts of rhizoma atractylodis, 4 parts of wrinkled giant hyssop, snake 6 parts of machine tool.
The sterilization powder fineness is 70 mesh.
The activated carbon is the mesh of granularity 70.
On the one hand a kind of method using its fermented stalk provided, the method using its fermented stalk includes as follows Step:
Step(1):By robust growth in subculture Multiple Buds, leaf leaf color is normal, and plant height about 1.5-2cm bud cuts down switching Taken root into the photinia glabra tissue-cultured seedling root media, cultivation temperature is 24-29 DEG C, and intensity of illumination is 2000- 2800Lux, light application time is daily 8 hours, obtains test tube seedling;
Step(2):By step(1)Gained test tube seedling is transplanted into perlite-peat soil mixed-matrix that volume ratio is 4: 8 and trained Support, produce photinia glabra tissue-cultured seedling.
Embodiment 4
A kind of photinia glabra tissue-cultured seedling root media that on the one hand the present embodiment provides, the photinia glabra tissue culture seedling rooting training Base is supported using MS culture mediums as minimal medium, sucrose 15g/L, activated carbon 2.55g/L, organic fertilizer group are added in MS culture mediums Divide 1.6g/L, sterilize powder 2.6ml/L, mineral salt compositions 0.5g/L, heated with slow fire, and be stirred continuously, make said components molten Solution, adds 1mol/L hydrochloric acid regulation pH value, makes the pH value of the root media between 5.4-6.0, add 3-4g's It is put into after agar, stirring in pressurization autoclave, sterilize 20-25min under the conditions of 105-121 DEG C.
The organic fertilizer component formula is specific as follows:Wheat bran 3000g, lactic acid bacteria 500g, corn steep liquor 500g, water 50000g, rice bran 3000g, saccharomycete 500g, phosphate fertilizer 500g, nitrogenous fertilizer 6000g, marine alga 3000g, crushed bone meal 500g, fishbone dust 500g。
The mineral salt compositions formula is specific as follows:Ammonium nitrate 0.46g/L, calcium chloride 0.17g/L, inositol 0.1g/L, Potassium carbonate 2.83g/L, 1- methyl α-naphthyl acetate 2mg/L, sodium dihydrogen phosphate 0.4g/L, magnesium carbonate 0.19g/L.
The sterilization powder formula is specific as follows:6 parts of honeysuckle, 7 parts of the tuber of stemona, 6 parts of dittany bark, 5 parts of rhizoma atractylodis, 5 parts of wrinkled giant hyssop, snake 4 parts of machine tool.
The sterilization powder fineness is 80 mesh.
The activated carbon is the mesh of granularity 70.
On the one hand a kind of method using its fermented stalk provided, the method using its fermented stalk includes as follows Step:
Step(1):By robust growth in subculture Multiple Buds, leaf leaf color is normal, and plant height about 1.5-2cm bud cuts down switching Taken root into the photinia glabra tissue-cultured seedling root media, cultivation temperature is 24-29 DEG C, and intensity of illumination is 2000- 2800Lux, light application time is daily 8 hours, obtains test tube seedling;
Step(2):By step(1)Gained test tube seedling is transplanted into perlite-peat soil mixed-matrix that volume ratio is 4: 8 and trained Support, produce photinia glabra tissue-cultured seedling.
Embodiment 5
A kind of photinia glabra tissue-cultured seedling root media that on the one hand the present embodiment provides, the photinia glabra tissue culture seedling rooting training Base is supported using MS culture mediums as minimal medium, sucrose 17g/L, activated carbon 1.85g/L, organic fertilizer group are added in MS culture mediums Divide 1.6g/L, sterilize powder 2.5ml/L, mineral salt compositions 0.9g/L, heated with slow fire, and be stirred continuously, make said components molten Solution, adds 1mol/L hydrochloric acid regulation pH value, the pH value for making the root media is 5.6, adds 3-4g agar, is stirred After be put into pressurization autoclave in, sterilize 20min under the conditions of 105-121 DEG C.
The organic fertilizer component formula is specific as follows:Wheat bran 3000g, lactic acid bacteria 500g, corn steep liquor 500g, water 50000g, rice bran 3000g, saccharomycete 500g, phosphate fertilizer 500g, nitrogenous fertilizer 6000g, marine alga 3000g, crushed bone meal 500g, fishbone dust 500g。
The mineral salt compositions formula is specific as follows:Ammonium nitrate 0.46g/L, calcium chloride 0.17g/L, inositol 0.1g/L, Potassium carbonate 2.83g/L, 1- methyl α-naphthyl acetate 2mg/L, sodium dihydrogen phosphate 0.4g/L, magnesium carbonate 0.19g/L.
The sterilization powder formula is specific as follows:5-8 parts of honeysuckle, 5-8 parts of the tuber of stemona, 5-8 parts of dittany bark, 3-6 parts of rhizoma atractylodis, the leaves of pulse plants Fragrant 3-6 parts, 4-7 parts of frutus cnidii.
The sterilization powder fineness is 70 mesh.
The activated carbon is the mesh of granularity 70.
On the one hand a kind of method using its fermented stalk provided, the method using its fermented stalk includes as follows Step:
Step(1):By robust growth in subculture Multiple Buds, leaf leaf color is normal, and plant height about 1.5-2cm bud cuts down switching Taken root into the photinia glabra tissue-cultured seedling root media, cultivation temperature is 24-29 DEG C, and intensity of illumination is 2000- 2800Lux, light application time is daily 8 hours, obtains test tube seedling;
Step(2):By step(1)Gained test tube seedling is transplanted into perlite-peat soil mixed-matrix that volume ratio is 4: 8 and trained Support, produce photinia glabra tissue-cultured seedling.
Experimental example
(1)Robust growth in 6 groups of subculture Multiple Buds is taken, leaf leaf color is normal, plant height about 1.5-2cm photinia glabra bud;
(2)Wherein 5 groups are transferred to described in the present embodiment 1-5 on photinia glabra tissue-cultured seedling culture medium and cultivate 15 days, be left 1 group for pair It is transferred on ordinary culture medium and cultivates 15 days according to group, above-mentioned 6 groups of condition of culture is identical;
(3)Experimental result, is shown in Table 1.
The photinia glabra tissue-cultured seedling growing state list of table 1
Group Rootage duration Growing way
1 group After 3 days It is healthy and strong
2 groups After 4 days It is healthy and strong
3 groups After 3 days It is healthy and strong
4 groups After 4 days It is healthy and strong
5 groups After 3 days It is healthy and strong
Control group After 8 days Typically
Drawn by experimental result:A kind of photinia glabra tissue-cultured seedling root media that the present invention is provided, on the basis of MS culture mediums On, by adjusting the species and concentration of a great number of elements, optimization hormone regulating and controlling etc., equilibrium is provided for photinia glabra tissue culture rooted seedling Rational nutritional need, promotes its quick, efficient, robust growth.
The specific embodiment of the present invention is described above.It is to be appreciated that the invention is not limited in above-mentioned Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow Ring the substantive content of the present invention.

Claims (9)

1. a kind of photinia glabra tissue-cultured seedling root media, it is characterised in that the photinia glabra tissue-cultured seedling root media with MS culture mediums are minimal medium, and sucrose 10-19g/L, activated carbon 1.5-2.55g/L, organic fertilizer are added in MS culture mediums Component 1.5-1.7g/L, sterilizes powder 1.5-2.6ml/L, mineral salt compositions 0.5-1.8g/L, is heated with slow fire, and constantly stir Mix, dissolve said components, add 1mol/L hydrochloric acid regulation pH value, make the pH value of the root media 5.4-6.1 it Between, add and be put into after 3-6g agar, stirring in pressurization autoclave, sterilize 20-30min under the conditions of 105-121 DEG C.
2. photinia glabra tissue-cultured seedling root media according to claim 1, it is characterised in that the photinia glabra tissue culture Seedling rooting culture medium adds sucrose 15-19g/L, activated carbon 1.7- using MS culture mediums as minimal medium in MS culture mediums 2.55g/L, organic fertilizer component 1.6-1.7g/L, sterilize powder 1.9-2.6ml/L, mineral salt compositions 0.5-1.1g/L, with text Fire heating, and be stirred continuously, said components are dissolved, 1mol/L hydrochloric acid regulation pH value is added, makes the root media PH value is added and is put into after 3-4g agar, stirring in pressurization autoclave, gone out under the conditions of 105-121 DEG C between 5.4-6.0 Bacterium 20-25min.
3. photinia glabra tissue-cultured seedling root media according to claim 1, it is characterised in that the photinia glabra tissue culture Seedling rooting culture medium adds sucrose 17g/L, activated carbon 1.85g/L in MS culture mediums, had using MS culture mediums as minimal medium Machine fertilizer components 1.6g/L, sterilizes powder 2.5ml/L, mineral salt compositions 0.9g/L, is heated with slow fire, and is stirred continuously, and makes Component dissolving is stated, 1mol/L hydrochloric acid regulation pH value is added, the pH value for making the root media is 5.6, adds 3-4g's It is put into after agar, stirring in pressurization autoclave, sterilize 20min under the conditions of 105-121 DEG C.
4. photinia glabra tissue-cultured seedling root media according to claim 1, it is characterised in that the organic fertilizer component Formula is specific as follows:Wheat bran 3000g, lactic acid bacteria 500g, corn steep liquor 500g, water 50000g, rice bran 3000g, saccharomycete 500g, phosphate fertilizer 500g, nitrogenous fertilizer 6000g, marine alga 3000g, crushed bone meal 500g, fishbone dust 500g.
5. photinia glabra tissue-cultured seedling root media according to claim 1, it is characterised in that the mineral salt compositions Formula is specific as follows:Ammonium nitrate 0.46g/L, calcium chloride 0.17g/L, inositol 0.1g/L, potassium carbonate 2.83g/L, 1- methyl α-naphthyl acetate 2mg/L, sodium dihydrogen phosphate 0.4g/L, magnesium carbonate 0.19g/L.
6. photinia glabra tissue-cultured seedling root media according to claim 1, it is characterised in that the sterilization powder formula tool Body is as follows:5-8 parts of honeysuckle, 5-8 parts of the tuber of stemona, 5-8 parts of dittany bark, 3-6 parts of rhizoma atractylodis, 3-6 parts of wrinkled giant hyssop, 4-7 parts of frutus cnidii.
7. photinia glabra tissue-cultured seedling root media according to claim 1, it is characterised in that:It is described sterilization powder fineness be 60-90 mesh.
8. photinia glabra tissue-cultured seedling root media according to claim 1, it is characterised in that:The activated carbon is granularity 60-90 mesh.
9. the method for photinia glabra tissue-cultured seedling root media culture photinia glabra is utilized as described in claim any one of 1-8, It is characterized in that:Comprise the following steps:
Step(1):By robust growth in subculture Multiple Buds, leaf leaf color is normal, and plant height about 1.5-2cm bud cuts down switching Taken root into the photinia glabra tissue-cultured seedling root media, cultivation temperature is 24-29 DEG C, and intensity of illumination is 2000- 2800Lux, light application time is daily 8 hours, obtains test tube seedling;
Step(2):By step(1)Gained test tube seedling is transplanted into perlite-peat soil mixed-matrix that volume ratio is 4: 8 and trained Support, you can.
CN201710406768.2A 2017-06-02 2017-06-02 A kind of photinia glabra tissue-cultured seedling root media and cultural method Pending CN106962205A (en)

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CN102835314A (en) * 2012-09-20 2012-12-26 重庆文理学院 Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting
CN103430754A (en) * 2013-09-04 2013-12-11 句容市农业技术推广中心 Cuttage and rapid propagation technology of photinia serrulata
CN103918530A (en) * 2014-03-20 2014-07-16 芜湖晋诚农业科技有限公司 High-yield cowpea planting method
CN103918426A (en) * 2014-03-20 2014-07-16 芜湖晋诚农业科技有限公司 High-yield planting method for towel gourds
CN104429974A (en) * 2014-12-25 2015-03-25 安徽同创现代农业投资发展有限公司 Rooting culture medium for culturing dendrobium officinale plantlets
CN104429973A (en) * 2014-12-25 2015-03-25 安徽同创现代农业投资发展有限公司 Method of culturing dendrobium officinale plantlets
CN104488497A (en) * 2014-11-25 2015-04-08 安顺市西秀区春实绿化苗木有限公司 Photinia fraseri cuttage propagation method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102835314A (en) * 2012-09-20 2012-12-26 重庆文理学院 Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting
CN103430754A (en) * 2013-09-04 2013-12-11 句容市农业技术推广中心 Cuttage and rapid propagation technology of photinia serrulata
CN103918530A (en) * 2014-03-20 2014-07-16 芜湖晋诚农业科技有限公司 High-yield cowpea planting method
CN103918426A (en) * 2014-03-20 2014-07-16 芜湖晋诚农业科技有限公司 High-yield planting method for towel gourds
CN104488497A (en) * 2014-11-25 2015-04-08 安顺市西秀区春实绿化苗木有限公司 Photinia fraseri cuttage propagation method
CN104429974A (en) * 2014-12-25 2015-03-25 安徽同创现代农业投资发展有限公司 Rooting culture medium for culturing dendrobium officinale plantlets
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Application publication date: 20170721