CN104012412B - A kind of strengthening seedling and rooting substratum of Kiwifruit - Google Patents
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- 244000298697 Actinidia deliciosa Species 0.000 title claims abstract description 28
- 235000009436 Actinidia deliciosa Nutrition 0.000 title claims abstract description 28
- 238000005728 strengthening Methods 0.000 title claims abstract 4
- 239000008272 agar Substances 0.000 claims abstract description 6
- 239000005720 sucrose Substances 0.000 claims abstract description 6
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 9
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 239000011785 micronutrient Substances 0.000 claims description 6
- 235000013369 micronutrients Nutrition 0.000 claims description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 3
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 claims description 3
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 3
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- 229960000367 inositol Drugs 0.000 claims description 3
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 3
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 3
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 3
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 3
- 229960003512 nicotinic acid Drugs 0.000 claims description 3
- 235000001968 nicotinic acid Nutrition 0.000 claims description 3
- 239000011664 nicotinic acid Substances 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 235000007715 potassium iodide Nutrition 0.000 claims description 3
- 239000004323 potassium nitrate Substances 0.000 claims description 3
- 235000010333 potassium nitrate Nutrition 0.000 claims description 3
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 claims description 3
- 229960004172 pyridoxine hydrochloride Drugs 0.000 claims description 3
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 claims description 3
- 239000011764 pyridoxine hydrochloride Substances 0.000 claims description 3
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 230000035764 nutrition Effects 0.000 claims 2
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims 1
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims 1
- 235000019796 monopotassium phosphate Nutrition 0.000 claims 1
- 229960004839 potassium iodide Drugs 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 150000003722 vitamin derivatives Chemical class 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- 239000012882 rooting medium Substances 0.000 description 11
- 229920001817 Agar Polymers 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- 235000021073 macronutrients Nutrition 0.000 description 4
- 238000009395 breeding Methods 0.000 description 2
- 230000003050 macronutrient Effects 0.000 description 2
- CDUFCUKTJFSWPL-UHFFFAOYSA-L manganese(II) sulfate tetrahydrate Chemical compound O.O.O.O.[Mn+2].[O-]S([O-])(=O)=O CDUFCUKTJFSWPL-UHFFFAOYSA-L 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 239000011684 sodium molybdate Substances 0.000 description 2
- 235000015393 sodium molybdate Nutrition 0.000 description 2
- 229960000344 thiamine hydrochloride Drugs 0.000 description 2
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 2
- 239000011747 thiamine hydrochloride Substances 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229940052299 calcium chloride dihydrate Drugs 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- BDRTVPCFKSUHCJ-UHFFFAOYSA-N molecular hydrogen;potassium Chemical compound [K].[H][H] BDRTVPCFKSUHCJ-UHFFFAOYSA-N 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
技术领域technical field
本发明涉及一种猕猴桃的壮苗生根培养基，属于植物学技术领域。The invention relates to a rooting medium for strong seedlings of kiwifruit, belonging to the technical field of botany.
背景技术Background technique
猕猴桃的茎枝上容易产生不定根，跟上容易产生不定芽，适于猕猴桃繁殖的方式有很多种，如，扦插繁殖、压条繁殖、嫁接繁殖和种子繁殖等。上述繁殖方法具有繁殖周期长、易感染真菌性病害、良种化程度降低，而劣质种苗的使用又导致猕猴桃的果实质量及营养价值降低，同时口感下降，这在一定程度上约束了猕猴桃的大力发展和推广应用。Adventitious roots are easy to be produced on the stems of kiwifruit, and adventitious buds are easy to be produced on the stems and branches of kiwifruit. There are many ways suitable for kiwifruit propagation, such as cutting propagation, layering propagation, grafting propagation and seed propagation. The above-mentioned breeding method has the advantages of long breeding cycle, susceptibility to fungal diseases, and reduced degree of improved varieties, while the use of inferior seedlings leads to a reduction in the fruit quality and nutritional value of kiwifruit, and a decrease in taste, which to a certain extent restricts the vigorous development of kiwifruit. Develop and promote applications.
发明内容Contents of the invention
为了克服现有技术的不足，本发明的目的在于提供一种培育周期短、可有效降低猕猴桃的染病几率，提高其生根成活率的猕猴桃的壮苗生根培养基。In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a rooting medium for strong seedlings of kiwifruit with a short cultivation period, which can effectively reduce the disease probability of kiwifruit and improve the survival rate of rooting.
为了实现上述发明目的，本发明是通过以下技术方案来实现的：In order to achieve the above-mentioned purpose of the invention, the present invention is achieved through the following technical solutions:
一种猕猴桃的壮苗生根培养基，其特征在于，包括包括1/2改良MS+0.5～1.0mgL-1BA+0.3～0.7mgL-1IAA+3～5gL-1琼脂+20gL-1蔗糖,且pH值为5.8。A rooting medium for strong seedlings of kiwifruit, characterized in that it includes 1/2 improved MS+0.5-1.0mgL -1 BA+0.3-0.7mgL -1 IAA+3-5gL -1 agar+20gL -1 sucrose, And the pH value is 5.8.
更进一步，所述的改良MS包括常量营养元素、微量营养元素和有机试剂。Furthermore, the improved MS includes macronutrients, micronutrients and organic reagents.
其中，所述的常量营养元素的组分和其对应的浓度为：硝酸钾1900mg/L；硫酸铵1650mg/L；七水硫酸镁350mg/L；二水氯化钙400mg/L；无水磷酸二氢钾150mg/L；乙二胺四乙酸二钠35.6mg/L；七水硫酸亚铁25.8mg/L。Wherein, the components of the macronutrient elements and their corresponding concentrations are: potassium nitrate 1900mg/L; ammonium sulfate 1650mg/L; magnesium sulfate heptahydrate 350mg/L; calcium chloride dihydrate 400mg/L; anhydrous phosphoric acid Potassium dihydrogen 150mg/L; disodium edetate 35.6mg/L; ferrous sulfate heptahydrate 25.8mg/L.
所述的微量营养元素的组分和其对应的浓度为：四水硫酸锰20.5mg/L；硫酸锌9.0mg/L；硼酸6.5mg/L；碘化钾0.5mg/L；钼酸钠0.3mg/L；氯化钴0.05mg/L，硫酸铜0.02mg/L，The components of the micronutrient elements and their corresponding concentrations are: manganese sulfate tetrahydrate 20.5mg/L; zinc sulfate 9.0mg/L; boric acid 6.5mg/L; potassium iodide 0.5mg/L; sodium molybdate 0.3mg/L L; cobalt chloride 0.05mg/L, copper sulfate 0.02mg/L,
而所述的有机试剂的组分和其对应的浓度为：盐酸硫胺素10.0mg/L；烟酸1.5mg/L；盐酸吡哆醇1.5mg/L；肌醇80.0mg/L。The components of the organic reagent and their corresponding concentrations are: thiamine hydrochloride 10.0 mg/L; nicotinic acid 1.5 mg/L; pyridoxine hydrochloride 1.5 mg/L; inositol 80.0 mg/L.
本发明的有益效果是：本发明所述的猕猴桃的壮苗生根培养基通过改良成分和含量，调整培养条件，使得猕猴桃的移栽成活率可达到90％以上，种苗生长健壮，可有效提高猕猴桃的产量和质量，增强了其经济价值。The beneficial effects of the present invention are: the rooting medium for strong seedlings of kiwifruit described in the present invention adjusts the culture conditions by improving the composition and content, so that the transplanting survival rate of kiwifruit can reach more than 90%, the seedlings grow robustly, and can effectively improve The yield and quality of kiwifruit have enhanced its economic value.
具体实施方式Detailed ways
下面将结合具体实施例，详细说明本发明的具体实施方式：The specific implementation manner of the present invention will be described in detail below in conjunction with specific embodiment:
在本实施方式中，所有实施例中采用的改良MS均包括常量营养元素、微量营养元素和有机试剂，In this embodiment, the modified MS used in all examples includes macronutrients, micronutrients and organic reagents,
且所述的常量营养元素的组分和其对应的浓度为：硝酸钾1900mg/L；硫酸铵1650mg/L；七水硫酸镁350mg/L；二水氯化钙400mg/L；无水磷酸二氢钾150mg/L；乙二胺四乙酸二钠35.6mg/L；七水硫酸亚铁25.8mg/L。And the component of described macronutrient element and its corresponding concentration are: potassium nitrate 1900mg/L; Ammonium sulfate 1650mg/L; Magnesium sulfate heptahydrate 350mg/L; Dihydrate calcium chloride 400mg/L; Potassium hydrogen 150mg/L; disodium edetate 35.6mg/L; ferrous sulfate heptahydrate 25.8mg/L.
所述的微量营养元素的组分和其对应的浓度为：四水硫酸锰20.5mg/L；硫酸锌9.0mg/L；硼酸6.5mg/L；碘化钾0.5mg/L；钼酸钠0.3mg/L；氯化钴0.05mg/L，硫酸铜0.02mg/L，The components of the micronutrient elements and their corresponding concentrations are: manganese sulfate tetrahydrate 20.5mg/L; zinc sulfate 9.0mg/L; boric acid 6.5mg/L; potassium iodide 0.5mg/L; sodium molybdate 0.3mg/L L; cobalt chloride 0.05mg/L, copper sulfate 0.02mg/L,
而所述的有机试剂的组分和其对应的浓度为：盐酸硫胺素10.0mg/L；烟酸1.5mg/L；盐酸吡哆醇1.5mg/L；肌醇80.0mg/L。The components of the organic reagent and their corresponding concentrations are: thiamine hydrochloride 10.0 mg/L; nicotinic acid 1.5 mg/L; pyridoxine hydrochloride 1.5 mg/L; inositol 80.0 mg/L.
实施例1:Example 1:
猕猴桃的壮苗生根培养基，包括包括1/2改良MS+0.5mgL-1BA+0.3mgL-1IAA+3gL-1琼脂+20gL-1蔗糖,且pH值为5.8。The strong seedling rooting medium of kiwifruit includes 1/2 improved MS+0.5mgL -1 BA+0.3mgL -1 IAA+3gL -1 agar+20gL -1 sucrose, and the pH value is 5.8.
实施例2：Example 2:
猕猴桃的壮苗生根培养基，包括包括1/2改良MS+1.0mgL-1BA+0.7mgL-1IAA+5gL-1琼脂+20gL-1蔗糖,且pH值为5.8。The strong seedling rooting medium of kiwifruit includes 1/2 improved MS+1.0mgL -1 BA+0.7mgL -1 IAA+5gL -1 agar+20gL -1 sucrose, and the pH value is 5.8.
实施例3：Example 3:
猕猴桃的壮苗生根培养基，包括包括1/2改良MS+0.8mgL-1BA+0.5mgL-1IAA+4gL-1琼脂+20gL-1蔗糖,且pH值为5.8。The strong seedling rooting medium of kiwifruit includes 1/2 improved MS+0.8mgL -1 BA+0.5mgL -1 IAA+4gL -1 agar+20gL -1 sucrose, and the pH value is 5.8.
实施例4：Example 4:
利用实施例1-3所述的猕猴桃的壮苗生根培养基对猕猴桃进行生根培养，具体步骤为：将外植体大量增殖后的无菌试管苗转移到壮苗生根培养基中诱导生根，培养25-30d，至根长至2.0cm以上时，进行炼苗移栽猕猴桃的增殖继代后的外植体进行壮苗生根培养，具体步骤如下：将增殖继代培养后的试管苗首先在培养室适应1d，然后在温室条件下，清水洗涤4次后移栽到装有混合育苗基质的穴盘中，保持空气相对湿度在80％以上，然后驯化炼苗12d后移栽进行常规管理。Utilize the strong seedling rooting medium of kiwifruit described in embodiment 1-3 to carry out rooting culture to kiwifruit, and concrete steps are: transfer the aseptic test-tube plantlet after the explant proliferates in the strong seedling rooting medium to induce rooting, cultivate 25-30d, when the root grows to more than 2.0cm, carry out hardening and transplanting the explant after the proliferation of kiwi fruit to carry out strong seedling rooting culture, concrete steps are as follows: the test-tube plantlet after the proliferation and subculture is first cultivated After acclimatization in the room for 1 day, they were washed with water for 4 times under greenhouse conditions and then transplanted into plug trays with mixed seedling-raising substrates. The relative humidity of the air was kept above 80%.
利用上述实施例1-3的壮苗生根培养基培养猕猴桃的实验结果如表1所示：Utilize the strong seedling rooting medium of above-mentioned embodiment 1-3 to cultivate the experimental result of kiwi fruit as shown in table 1:
表1：猕猴桃壮苗生根培养的结果Table 1: Results of rooting culture of kiwifruit strong seedlings
由表1可知：改良壮苗生根培养基的成分以及含量，使得猕猴桃的移栽成活率可达到90％以上，种苗生长健壮，可有效提高猕猴桃的产量和质量，增强了其经济价值。It can be seen from Table 1 that the composition and content of the rooting medium for strong seedlings can be improved, so that the transplanting survival rate of kiwifruit can reach more than 90%, and the seedlings grow robustly, which can effectively improve the yield and quality of kiwifruit, and enhance its economic value.
以上已以较佳实施例公开了本发明，然其并非用以限制本发明，凡采用等同替换或者等效变换方式所获得的技术方案，均落在本发明的保护范围之内。The above has disclosed the present invention with preferred embodiments, but it is not intended to limit the present invention, and all technical solutions obtained by adopting equivalent replacement or equivalent transformation methods fall within the protection scope of the present invention.
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| CN107372113A (en) * | 2017-08-07 | 2017-11-24 | 镇江万山红遍农业园 | A kind of peach is colonized root media |
| CN108586003A (en) * | 2018-05-21 | 2018-09-28 | 绥阳县瓦房水榭农旅发展有限公司 | Organic fertilizer and preparation method thereof for cultivating red kiwifruit |
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Effective date of registration: 20180802 Address after: 341600 Changan village, oil Hill Town, Xinfeng County, Ganzhou, Jiangxi Patentee after: Jiangxi Chu Chu Fruit Industry Co., Ltd. Address before: 325200 20, Lane 1, Fei Yun Road, Fei Yun street, Ruian, Wenzhou, Zhejiang. Patentee before: Zhao Lan |