CN102824949B - Remotely-controlled automatically-lifted clamping device of ultrasonic cell crusher - Google Patents

Remotely-controlled automatically-lifted clamping device of ultrasonic cell crusher Download PDF

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Publication number
CN102824949B
CN102824949B CN201110158885.4A CN201110158885A CN102824949B CN 102824949 B CN102824949 B CN 102824949B CN 201110158885 A CN201110158885 A CN 201110158885A CN 102824949 B CN102824949 B CN 102824949B
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China
Prior art keywords
test tube
pallet
tube clamp
integrated
drive motor
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Expired - Fee Related
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CN201110158885.4A
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CN102824949A (en
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邵家胜
卢洪洲
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Fudan University
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Fudan University
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Abstract

The invention belongs to the field of research equipment of molecular biology, and relates to a remotely-controlled automatically-lifted clamping device of an ultrasonic cell crusher. The clamping device mainly comprises a pedestal, a tray driving integration, a test tube holder driving integration, an adjusting screw knob of the test tube holder driving integration, pedestal installation screws, and fastening screws of the tray driving integration, wherein the pedestal is fixed on an inner base plate of a sound insulation box through the pedestal installation screws; the tray driving integration is inserted into the pedestal and fixed by the fastening screws of the tray driving integration; and the test tube holder driving integration is sleeved above the tray driving integration and rotates relative to the tray driving integration, wherein the adjusting screw knob of the test tube holder driving integration adjusts and fixes the relative positions of the test tube holder driving integration. The device is simple in structure, convenient to control, stable and reliable, and can automatically and accurately control up-and-down movement and positioning of the test tube holder and an objective table, reduce trouble and inconvenience of operation and improve working efficiency.

Description

Remote control automatic-lifting holding device for ultrasonic cell crusher
Technical field
The invention belongs to molecular biology research instrument and equipment field, relate to the instrument and equipment in a kind of molecular biology research field, be specifically related to a kind of remote control automatic-lifting holding device for ultrasonic cell crusher, described clamping device is the remote control automatic-lifting holding device of ultrasonic cell disruptor sound proof box inside.
Background technology
Ultrasonic cell disruptor is a kind of multi-functional, multipurpose instrument that experimental subjects is carried out to ultrasonic processing, it has broken broken tissue, bacterium, virus, spore and other cyto-architectural function, simultaneously, can be used to homogeneous, emulsification, mixing, degassed, collapse loose, disperse, leach and extract, accelerate reflection etc., therefore be widely used in the experimental study field of biology, medical science, chemistry, pharmacy, food, cosmetics, environmental protection etc.
Before carrying out ultrasonic wave pulverizing sample, experimenter need to pack sample into test tube or beaker, is then placed on the test tube clamp or objective table of sound proof box inside; Because test tube clamp and objective table in existing clamping device are to be all fixed on elevating lever by spiral shell button, experimenter need to stretch into hand sound proof box inside, carry out the height of fine tuning objective table or test tube clamp by spiral shell button, thereby control the degree of depth of ultrasonic transformer insertion sample liquid level.In practical operation, find, existing clamping device has many weak points: on the one hand, objective table or test tube clamp need to repeatedly regulate just can reach desirable height, repeatedly operates spiral shell button extremely loaded down with trivial details and inconvenient by craft, on the other hand, sound proof box inner space is narrow, and machine is in the time of work, at a large amount of heat energy of the local convergence collection of stoste, may reduce or the activity of deactivation product, therefore need constantly to open sound proof box and stir stoste, or be placed in the container that fills mixture of ice and water, therefore be easy to sample to overturn in frequent operation process, have a strong impact on the yield of product, even cause the failure of experiment, particularly for some precious sample, as the stoste of protein purification and extracting plasmid, as caused the failure of an experiment because of operate miss, can cause larger economic loss.In addition, spiral shell button in existing clamping device is long-time to be also easy to get rusty after using, and causes objective table or test tube clamp to be difficult for being fixed on elevating lever and often landing, and the biological sample that therefore has latent infection for some easily causes bio-safety hidden danger.
As can be seen here, adopt the clamping device with function of auto-lift can effectively avoid above-mentioned deficiency, there is very large reality and the market development and be worth.At present the document of prior art is retrieved, not yet found to have the ultrasonic grinder clamping device of function of auto-lift.
Summary of the invention
The defect and the deficiency that the object of the invention is to overcome prior art, provide a kind of remote control automatic-lifting holding device for ultrasonic cell crusher; In described clamping device, remote control test tube clamp and objective table move up and down, and automatically regulate the height of objective table or test tube clamp, reduce manually operated loaded down with trivial details and inconvenient, make operation more reliable and more stable.
Particularly, remote control automatic-lifting holding device for ultrasonic cell crusher of the present invention, it is characterized in that, mainly comprise that base 1, pallet drive integrated 2, test tube clamp to drive integrated 3, test tube clamp to drive integrated adjusting spiral shell knob 4, floor installation screw 5 and pallet to drive integrated trip bolt 6; Described base 1 is fixed on sound proof box internal layer base plate by floor installation screw 5; Described pallet drives in integrated 2 inserted bases 1, drives integrated trip bolt 6 fixing by pallet; Described test tube clamp drives integrated 3 to be enclosed within pallet and to drive integrated 2 top, and test tube clamp drives integrated 3 to drive integrated 2 to rotate relative to pallet, drives integrated adjusting spiral shell knob 4 adjust and fix its relative position by test tube clamp.
In the present invention, described pallet drives integrated 2 to be made up of outer tube 7, pallet 8, nut 9, leading screw 10, shaft coupling 11, pallet drive motor 12, inner layer pipe 13, pallet drive motor mounting screw 14, upper bearing (metal) 15, upper bearing sleeve 16, upper bearing sleeve trip bolt 17, lower bearing 18, lower bearing sleeve 19, lower bearing sleeve trip bolt 20 and ectonexine pipe trip bolt 21;
In described outer tube 7, have two parallel guide slots, pallet 8 is enclosed within outer tube 7, is limited by guide groove, can only move up and down; Nut 9 is embedded in pallet 8 by interference fit, and nut 9 is enclosed within on screw mandrel 10; Screw mandrel 10 lower ends are fixed on 18 li of lower bearings, and screw mandrel 10 upper ends, through upper bearing (metal) 15, are connected with shaft coupling 11 one end, and shaft coupling 11 other ends are connected with the rotating shaft of pallet drive motor 12; Lower bearing 18 is arranged in lower bearing sleeve 19, and lower bearing sleeve 19 is fixed on outer tube 7 inner bottom parts by lower bearing sleeve screw 20, and upper bearing (metal) 15 is arranged in upper bearing sleeve 16, and upper bearing sleeve 16 is fixed on outer tube 7 internal upper parts by upper bearing sleeve screw 17; In inner layer pipe 13, have dividing plate with holes, be divided into upper and lower cavity, pallet drive motor 12 is fixed in inner layer pipe 13 epicoeles by pallet drive motor mounting screw 14; Inner layer pipe 13 bottoms are stretched in outer tube 7, are supported on upper bearing sleeve 16, limit its rotation by ectonexine pipe trip bolt 21.
In the present invention, described test tube clamp drives integrated 3 to regulate spiral shell knob 33 to form by stay pipe 22, left socle 23, support linking bolt 24, right support 25, tooth bar 26, tooth bar trip bolt 27, test tube clamp drive motor 28, gear 29, test tube clamp 30, enclosing cover trip bolt 31, enclosing cover 32 and test tube clamp;
Described stay pipe 22 is the non-complete pipe with chute, and left socle 23 is connected bolt 24 with right support 25 by support and is connected, and forms the support that goes in ring, and is enclosed within on stay pipe 22, can freely move up and down, and can not rotate relative to stay pipe 22 by chute constraint; Tooth bar 26 is fixed on stay pipe 22 sidewalls by tooth bar trip bolt 27; Test tube clamp drive motor 28 is arranged on right support 25, and the rotating shaft of test tube clamp drive motor 28 is coaxially fixedly connected with gear 29; Gear 29 engages with tooth bar 26; On left socle 23, have test tube clamp installing hole, insert in the test tube clamp installing hole on left socles 23 test tube clamp 30 one end, scalable motion with rotatablely move, regulate spiral shell knob 33 to adjust and the position of fixing test tube clamp 30 by test tube clamp; Enclosing cover 32 is enclosed within one end of left socle 23 and right support 25, and fixing with enclosing cover trip bolt 31, test tube clamp drive motor 28, gear 29 are encapsulated in enclosing cover 32.
When use, the rotating shaft of the pallet drive motor 12 described in the present invention is rotated, and drives screw mandrel 10 to rotate by shaft coupling 11, and screw mandrel 10 is by driving nut 9 to drive pallet 8 to move; Control the rotating shaft forward or reverse of pallet drive motor 12, realize the control that pallet 8 is risen or declined; The rotating shaft of described test tube clamp drive motor 28 is rotated, driven gear 29 rotates, gear 29 is by engaging with tooth bar 26, the motion of drive support, by controlling the rotating shaft forward or reverse of test tube clamp drive motor 28, realize the rise and fall campaign of support, test tube clamp 30 also rises or descending motion with support.
The present invention is simple in structure, and it is convenient to control, reliable and stable, can automatic accurate control test tube clamp and the moving up and down and locating of objective table.Adopt apparatus for automatically lifting to replace manual operation, reduced to a great extent the loaded down with trivial details and inconvenient of operation, greatly improve experimenter's operating efficiency, particularly can shorten experiment new hand's training time, make it enter fast experimental state.
For the ease of understanding, below the drawings and Examples by concrete are described in detail remote control automatic-lifting holding device for ultrasonic cell crusher of the present invention.It needs to be noted, instantiation and accompanying drawing are only in order to illustrate, obviously those of ordinary skill in the art can, according to explanation herein, make various corrections and change to the present invention within the scope of the invention, and these corrections and change are also included in scope of the present invention.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is that pallet of the present invention drives integrated grade for axis side view.
Fig. 3 is that pallet of the present invention drives integrated top view.
Fig. 4 is that pallet of the present invention drives integrated A-A cutaway view.
Fig. 5 is that test tube clamp of the present invention drives integrated grade for axis side view.
Fig. 6 is that test tube clamp of the present invention drives axis side views such as integrated (removal enclosing covers).
Fig. 7 is scheme of installation of the present invention.
In Fig. 1-Fig. 7, the 1st, base, the 2nd, pallet drives integrated, the 3rd, test tube clamp drives integrated, the 4th, test tube clamp drives integrated adjusting spiral shell knob, the 5th, floor installation screw, the 6th, pallet drives integrated trip bolt, the 7th, outer tube, the 8th, pallet, the 9th, nut, the 10th, leading screw, the 11st, shaft coupling, the 12nd, pallet drive motor, the 13rd, inner layer pipe, the 14th, pallet drive motor mounting screw, the 15th, upper bearing (metal), the 16th, upper bearing sleeve, the 17th, upper bearing sleeve trip bolt, the 18th, lower bearing, the 19th, lower bearing sleeve, the 20th, lower bearing sleeve trip bolt, the 21st, ectonexine pipe trip bolt, the 22nd, stay pipe, the 23rd, left socle, the 24th, support is connected bolt, the 25th, right support, the 26th, tooth bar, the 27th, tooth bar trip bolt, the 28th, test tube clamp drive motor, the 29th, gear, the 30th, test tube clamp, the 31st, enclosing cover trip bolt, the 32nd, enclosing cover, the 33rd, test tube clamp regulates spiral shell knob.
The specific embodiment
Embodiment 1
As shown in Fig. 1-6, this remote control automatic-lifting holding device for ultrasonic cell crusher, mainly comprises that base 1, pallet drive integrated 2, test tube clamp to drive integrated 3, test tube clamp to drive integrated adjusting spiral shell knob 4, floor installation screw 5 and pallet to drive integrated trip bolt 6; Described base 1 is fixed on sound proof box internal layer base plate by floor installation screw 5; Described pallet drives in integrated 2 inserted bases 1, drives integrated trip bolt 6 fixing by pallet; Described test tube clamp drives integrated 3 to be enclosed within pallet and to drive integrated 2 top, and test tube clamp drives integrated 3 to drive integrated 2 to rotate relative to pallet, drives integrated adjusting spiral shell knob 4 adjust and fix its relative position by test tube clamp.
Wherein, described pallet drives integrated 2 to be made up of outer tube 7, pallet 8, nut 9, leading screw 10, shaft coupling 11, pallet drive motor 12, inner layer pipe 13, pallet drive motor mounting screw 14, upper bearing (metal) 15, upper bearing sleeve 16, upper bearing sleeve trip bolt 17, lower bearing 18, lower bearing sleeve 19, lower bearing sleeve trip bolt 20 and ectonexine pipe trip bolt 21; In described outer tube 7, have two parallel guide slots, pallet 8 is enclosed within outer tube 7, is limited by guide groove, can only move up and down; Nut 9 is embedded in pallet 8 by interference fit, and nut 9 is enclosed within on screw mandrel 10; Screw mandrel 10 lower ends are fixed on 18 li of lower bearings, and screw mandrel 10 upper ends, through upper bearing (metal) 15, are connected with shaft coupling 11 one end, and shaft coupling 11 other ends are connected with the rotating shaft of pallet drive motor 12; Lower bearing 18 is arranged in lower bearing sleeve 19, and lower bearing sleeve 19 is fixed on outer tube 7 inner bottom parts by lower bearing sleeve screw 20, and upper bearing (metal) 15 is arranged in upper bearing sleeve 16, and upper bearing sleeve 16 is fixed on outer tube 7 internal upper parts by upper bearing sleeve screw 17; In inner layer pipe 13, have dividing plate with holes, be divided into upper and lower cavity, pallet drive motor 12 is fixed in inner layer pipe 13 epicoeles by pallet drive motor mounting screw 14; Inner layer pipe 13 bottoms are stretched in outer tube 7, are supported on upper bearing sleeve 16, limit its rotation by ectonexine pipe trip bolt 21.
Described test tube clamp drives integrated 3 to regulate spiral shell knob 33 to form by stay pipe 22, left socle 23, support linking bolt 24, right support 25, tooth bar 26, tooth bar trip bolt 27, test tube clamp drive motor 28, gear 29, test tube clamp 30, enclosing cover trip bolt 31, enclosing cover 32 and test tube clamp; Described stay pipe 22 is the non-complete pipe with chute, and left socle 23 is connected bolt 24 with right support 25 by support and is connected, and forms the support that goes in ring, and is enclosed within on stay pipe 22, can freely move up and down, and can not rotate relative to stay pipe 22 by chute constraint; Tooth bar 26 is fixed on stay pipe 22 sidewalls by tooth bar trip bolt 27; Test tube clamp drive motor 28 is arranged on right support 25, and the rotating shaft of test tube clamp drive motor 28 is coaxially fixedly connected with gear 29; Gear 29 engages with tooth bar 26; On left socle 23, have test tube clamp installing hole, insert in the test tube clamp installing hole on left socles 23 test tube clamp 30 one end, scalable motion with rotatablely move, regulate spiral shell knob 33 to adjust and the position of fixing test tube clamp 30 by test tube clamp; Enclosing cover 32 is enclosed within one end of left socle 23 and right support 25, and fixing with enclosing cover trip bolt 31, test tube clamp drive motor 28, gear 29 are encapsulated in enclosing cover 32.
As shown in Figure 7, this remote control automatic-lifting holding device is fixed on the internal layer base plate of ultrasonic cell disruptor sound proof box.
When use, the rotating shaft of the pallet drive motor 12 of this remote control automatic-lifting holding device is rotated, and drives screw mandrel 10 to rotate by shaft coupling 11, and screw mandrel 10 is by driving nut 9 to drive pallet 8 to move; Control the rotating shaft forward or reverse of pallet drive motor 12, realize the control that pallet 8 is risen or declined; The rotating shaft of described test tube clamp drive motor 28 is rotated, driven gear 29 rotates, gear 29 is by engaging with tooth bar 26, the motion of drive support, by controlling the rotating shaft forward or reverse of test tube clamp drive motor 28, realize the rise and fall campaign of support, test tube clamp 30 also rises or descending motion with support.
Embodiment 2
In the time that the sample size of ultrasonic cell disruptor single treatment is less, only need to just can meet requirement of experiment with test tube, now must be combined with pallet 8 by test tube clamp 30, operation comprises the following steps:
(1) test tube that sample is housed is fixed in test tube clamp 30;
(2) unscrew test tube clamp and regulate spiral shell knob 33 and test tube clamp to drive integrated adjusting spiral shell knob 4, it is integrated 3 that rotation test tube clamp drives, and simultaneous retractable and rotation test tube clamp 30, adjust the test tube position in test tube clamp 30, makes test tube and ultrasonic transformer concentric;
(3) tighten test tube clamp and regulate spiral shell knob 33 and test tube clamp to drive integrated adjusting spiral shell knob 4, test tube clamp 30 is fixed on left socle 23, test tube clamp drives integrated 3 the relative pallet of stay pipe 22 to drive integrated 2 outer tube 7 to fix;
(4) control test tube clamp drive motor 28 and rotate, by gear 29 tooth bar 26 transmissions, make left socle 23 drive the test tube in test tube clamp 30 to rise or decline, make the bottom of ultrasonic transformer be immersed in the following about 1-1.5cm of invisible spectro sample liquid level;
(5) stop test tube clamp drive motor 28, make test tube remain on current location;
(6) beaker of putting in advance a certain amount of mixture of ice and water is placed on pallet 8, makes beaker and test tube concentric;
(7) control pallet drive motor 12 and rotate, by screw mandrel 10 nut 9 transmissions, drive the beaker on pallet 8 to rise, the part that in vitro holds sample is completely infused in mixture of ice and water;
(8) stop pallet drive motor 12;
(9) test tube beaker automatic lifting process completes, and can start supersonic cell and pulverize experiment, until experiment finishes;
(10) by above-mentioned contrary process, take off beaker and test tube;
(11) finish.
Embodiment 3
In the time that the sample size of single treatment is more, (such as 50ml), can only test with beaker, only needs to use separately pallet 8, and without test tube clamp, operation comprises the following steps:
(1) unscrew test tube clamp and regulate spiral shell knob 33, take off test tube clamp;
(2) sample is packed in the beaker of 100ml, then put into the larger beaker that mixture of ice and water is housed (noting not making it floating), be placed in the lump on pallet 8, make beaker and ultrasonic transformer concentric;
(3) control pallet drive motor 12 and rotate, by screw mandrel 10 nut 9 transmissions, drive two beakers on pallet 8 to rise, make the bottom of ultrasonic transformer be immersed in the following about 1-1.5cm of sample liquid level in beaker;
(4) stop pallet drive motor 12;
(5) beaker automatic lifting process completes, and can start supersonic cell and pulverize experiment, until experiment finishes;
(6) control pallet drive motor 12 and rotate, by screw mandrel 10 nut 9 transmissions, drive two beakers on pallet 8 to drop to initial position, take off beaker;
(7) load onto test tube clamp 30, tighten test tube clamp and regulate spiral shell knob 33;
(8) finish.

Claims (3)

1. a remote control automatic-lifting holding device for ultrasonic cell crusher, it is characterized in that, mainly comprise that base (1), pallet drive integrated (2), test tube clamp to drive integrated (3), test tube clamp to drive integrated adjusting spiral shell knob (4), floor installation screw (5) and pallet to drive integrated trip bolt (6); Described base (1) is fixed on sound proof box internal layer base plate by floor installation screw (5); Described pallet drives in integrated (2) inserted bases (1), drives integrated trip bolt (6) fixing by pallet; Described test tube clamp drives integrated (3) to be enclosed within the top of pallet driving integrated (2), test tube clamp drives integrated (3) to drive integrated (2) to rotate relative to pallet, is driven integrated adjusting spiral shell knob (4) to adjust and is fixed its relative position by test tube clamp.
2. by remote control automatic-lifting holding device for ultrasonic cell crusher claimed in claim 1, it is characterized in that, described pallet drives integrated (2) by outer tube (7), pallet (8), nut (9), leading screw (10), shaft coupling (11), pallet drive motor (12), inner layer pipe (13), pallet drive motor mounting screw (14), upper bearing (metal) (15), upper bearing sleeve (16), upper bearing sleeve trip bolt (17), lower bearing (18), lower bearing sleeve (19), lower bearing sleeve trip bolt (20) and ectonexine pipe trip bolt (21) composition,
In described outer tube (7), have two parallel guide slots, it is upper that pallet (8) is enclosed within outer tube (7), limited by guide groove, can only move up and down; Nut (9) is embedded in pallet (8) by interference fit, and nut (9) is enclosed within on screw mandrel (10); It is inner that screw mandrel (10) lower end is fixed on lower bearing (18), screw mandrel (10) upper end is through upper bearing (metal) (15), be connected with shaft coupling (11) one end, shaft coupling (11) other end is connected with the rotating shaft of pallet drive motor (12); Lower bearing (18) is arranged in lower bearing sleeve (19), lower bearing sleeve (19) is fixed on outer tube (7) inner bottom part by lower bearing sleeve screw (20), upper bearing (metal) (15) is arranged in upper bearing sleeve (16), and upper bearing sleeve (16) is fixed on outer tube (7) internal upper part by upper bearing sleeve screw (17); Inner layer pipe has dividing plate with holes in (13), is divided into upper and lower cavity, and pallet drive motor (12) is fixed in inner layer pipe (13) epicoele by pallet drive motor mounting screw (14); Inner layer pipe (13) bottom is stretched in outer tube (7), is supported on upper bearing sleeve (16) upper, limits its rotation by ectonexine pipe trip bolt (21).
3. by remote control automatic-lifting holding device for ultrasonic cell crusher claimed in claim 1, it is characterized in that, described test tube clamp drives integrated (3) to regulate spiral shell knob (33) to form by stay pipe (22), left socle (23), support linking bolt (24), right support (25), tooth bar (26), tooth bar trip bolt (27), test tube clamp drive motor (28), gear (29), test tube clamp (30), enclosing cover trip bolt (31), enclosing cover (32) and test tube clamp;
Described stay pipe (22) is the non-complete pipe with chute, left socle (23) is connected bolt (24) with right support (25) by support and is connected, form the support that goes in ring, be enclosed within on stay pipe (22), can freely move up and down, can not rotate relative to stay pipe (22) by chute constraint; Tooth bar (26) is fixed on stay pipe (22) sidewall by tooth bar trip bolt (27); It is upper that test tube clamp drive motor (28) is arranged on right support (25), and the rotating shaft of test tube clamp drive motor (28) is coaxially fixedly connected with gear (29); Gear (29) engages with tooth bar (26); Left socle has test tube clamp installing hole on (23), insert in the test tube clamp installing hole on left socle (23) test tube clamp (30) one end, scalable motion with rotatablely move, regulate spiral shell knob (33) to adjust and the position of fixing test tube clamp (30) by test tube clamp; Enclosing cover (32) is enclosed within one end of left socle (23) and right support (25), fixing with enclosing cover trip bolt (31), test tube clamp drive motor (28), gear (29) are encapsulated in enclosing cover (32).
CN201110158885.4A 2011-06-14 2011-06-14 Remotely-controlled automatically-lifted clamping device of ultrasonic cell crusher Expired - Fee Related CN102824949B (en)

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