CN102821843A - 色谱介质的制备方法 - Google Patents

色谱介质的制备方法 Download PDF

Info

Publication number
CN102821843A
CN102821843A CN2011800101971A CN201180010197A CN102821843A CN 102821843 A CN102821843 A CN 102821843A CN 2011800101971 A CN2011800101971 A CN 2011800101971A CN 201180010197 A CN201180010197 A CN 201180010197A CN 102821843 A CN102821843 A CN 102821843A
Authority
CN
China
Prior art keywords
ligand
shell
beads
buffer
inner core
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011800101971A
Other languages
English (en)
Chinese (zh)
Inventor
J·贝格斯特伦
B-L·约翰逊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
GE Healthcare Bio Sciences AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB filed Critical GE Healthcare Bio Sciences AB
Publication of CN102821843A publication Critical patent/CN102821843A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3291—Characterised by the shape of the carrier, the coating or the obtained coated product
    • B01J20/3293—Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28004—Sorbent size or size distribution, e.g. particle size
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
CN2011800101971A 2010-02-19 2011-02-16 色谱介质的制备方法 Pending CN102821843A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE1050157 2010-02-19
SE1050157-5 2010-02-19
PCT/SE2011/050165 WO2011102790A1 (en) 2010-02-19 2011-02-16 Method for production of chromatography media

Publications (1)

Publication Number Publication Date
CN102821843A true CN102821843A (zh) 2012-12-12

Family

ID=44483190

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011800101971A Pending CN102821843A (zh) 2010-02-19 2011-02-16 色谱介质的制备方法

Country Status (8)

Country Link
US (1) US8574437B2 (enExample)
EP (1) EP2536499A4 (enExample)
JP (1) JP2013520647A (enExample)
CN (1) CN102821843A (enExample)
AU (1) AU2011218487A1 (enExample)
CA (1) CA2789994A1 (enExample)
IN (1) IN2012DN06243A (enExample)
WO (1) WO2011102790A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104801278A (zh) * 2015-03-29 2015-07-29 武汉瑞法医疗器械有限公司 艾滋病毒亲和吸附剂的制备方法

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9278297B2 (en) * 2010-07-09 2016-03-08 Ge Healthcare Bio-Sciences Ab Method for ion-exchange chromatography and media used thereof
PL2748181T3 (pl) * 2011-09-30 2016-08-31 Ge Healthcare Bioprocess R&D Ab Sposób oczyszczania rozszczepionej proinsuliny
CN103418358A (zh) * 2012-05-15 2013-12-04 北京化工大学 一种复合配基的色谱介质
CN114917884A (zh) * 2014-03-14 2022-08-19 思拓凡生物工艺研发有限公司 用于生物颗粒纯化的分离基质
CN106795215A (zh) 2014-06-24 2017-05-31 通用电气医疗集团生物工艺研发股份公司 层析方法
US9647476B2 (en) 2014-09-16 2017-05-09 Navitas Semiconductor Inc. Integrated bias supply, reference and bias current circuits for GaN devices
GB201506117D0 (en) 2015-04-10 2015-05-27 Ge Healthcare Bio Sciences Ab Method for chromatography
GB201506113D0 (en) * 2015-04-10 2015-05-27 Ge Healthcare Bio Sciences Ab Method for chromatography
WO2017174422A1 (en) * 2016-04-06 2017-10-12 Ge Healthcare Bioprocess R&D Ab Chromatography matrix
WO2018063980A1 (en) 2016-09-29 2018-04-05 Bio-Rad Laboratories, Inc. Agarose-filled ceramic apatite
CN108178810B (zh) * 2016-12-07 2020-05-19 中国科学院大连化学物理研究所 一种反相/阴离子交换混合模式聚合物的制备及其应用
CN115135392A (zh) 2020-02-19 2022-09-30 生物辐射实验室股份有限公司 制备聚合物填充的色谱树脂的方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5135650A (en) * 1988-12-22 1992-08-04 Bio-Rad Laboratories, Inc. Chromatography stationary phase material for high performance liquid chromatography
US6428707B1 (en) * 1997-02-04 2002-08-06 Amersham Pharmacia Biotech Ab Adsorption/separation method and a medium for adsorption/separation
US6572766B1 (en) * 1997-03-04 2003-06-03 Amersham Biosciences Ab Matrices for separation and separation exploiting said matrices
US20040214157A1 (en) * 1994-06-29 2004-10-28 Simon C. Burton Chromatographic resins and methods for using same
US20050242037A1 (en) * 2002-08-27 2005-11-03 Hans Berg Chromatographic two-layer particles
WO2009131526A1 (en) * 2008-04-22 2009-10-29 Ge Healthcare Bio-Sciences Ab Chromatography medium

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5110784A (en) * 1984-04-09 1992-05-05 Dow Corning Corporation Dual surface porous material
JP2004532182A (ja) * 2000-12-31 2004-10-21 アメルシャム・バイオサイエンシーズ・アクチボラグ 低濃度の塩を含有する組成物の製造方法
US20040016702A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Device and method for purification of nucleic acids
SG131016A1 (en) * 2005-09-19 2007-04-26 Millipore Corp Asymmetric porous adsorptive bead
US20110155668A1 (en) * 2008-07-08 2011-06-30 Ge Healthcare Bio-Sciences Ab Separation medium for chromatography of various biomolecules
GB0814326D0 (en) * 2008-08-05 2008-09-10 Medical Res Council Phosphate binding materials and their uses

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5135650A (en) * 1988-12-22 1992-08-04 Bio-Rad Laboratories, Inc. Chromatography stationary phase material for high performance liquid chromatography
US20040214157A1 (en) * 1994-06-29 2004-10-28 Simon C. Burton Chromatographic resins and methods for using same
US6428707B1 (en) * 1997-02-04 2002-08-06 Amersham Pharmacia Biotech Ab Adsorption/separation method and a medium for adsorption/separation
US6572766B1 (en) * 1997-03-04 2003-06-03 Amersham Biosciences Ab Matrices for separation and separation exploiting said matrices
US20050242037A1 (en) * 2002-08-27 2005-11-03 Hans Berg Chromatographic two-layer particles
WO2009131526A1 (en) * 2008-04-22 2009-10-29 Ge Healthcare Bio-Sciences Ab Chromatography medium

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
F.TORIBIO，ET AL.: "Methods for chromatographic and electrophoretic separation and assay of NADP+ oxidoreductases", 《JOURNAL OF CHROMATOGRAPHY B：BIOMEDICAL APPLICATIONS》 *
L. A. AE. SLUYTERMAN, ET AL.: "CHROMATOFOCUSING IV. PROPERTIES OF AN AGAROSE POLYETHYLENEIMINE ION EX-CHANGER AND ITS SUITABILITY FOR PROTEIN SEPARATIONS", 《JOURNAL OF CHROMATOGRAPHY》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104801278A (zh) * 2015-03-29 2015-07-29 武汉瑞法医疗器械有限公司 艾滋病毒亲和吸附剂的制备方法

Also Published As

Publication number Publication date
EP2536499A4 (en) 2013-08-14
US20120309618A1 (en) 2012-12-06
IN2012DN06243A (enExample) 2015-09-25
JP2013520647A (ja) 2013-06-06
US8574437B2 (en) 2013-11-05
WO2011102790A1 (en) 2011-08-25
EP2536499A1 (en) 2012-12-26
CA2789994A1 (en) 2011-08-25
AU2011218487A1 (en) 2012-09-06

Similar Documents

Publication Publication Date Title
CN102821843A (zh) 色谱介质的制备方法
Sýkora et al. Recent advances in mixed‐mode chromatographic stationary phases
Li et al. Affinity monolith chromatography: A review of general principles and applications
Guihen et al. Recent highlights in stationary phase design for open-tubular capillary electrochromatography
Ueki et al. Preparation and application of methacrylate-based cation-exchange monolithic columns for capillary ion chromatography
EP3570974B1 (en) Multimodal chromatographic media for protein separation
Arrua et al. Review of recent advances in the preparation of organic polymer monoliths for liquid chromatography of large molecules
Connolly et al. Polymeric monolithic materials modified with nanoparticles for separation and detection of biomolecules: a review
Zajickova Advances in the development and applications of organic–silica hybrid monoliths
US9278297B2 (en) Method for ion-exchange chromatography and media used thereof
Liang et al. Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography
Krenkova et al. Iron oxide nanoparticle coating of organic polymer‐based monolithic columns for phosphopeptide enrichment
Mikšík Capillary electrochromatography of proteins and peptides (2006–2015)
Hajba et al. Recent advances in capillary electrochromatography of proteins and carbohydrates in the biopharmaceutical and biomedical field
Chmielowski et al. Resolution of heterogeneous charged antibody aggregates via multimodal chromatography: a comparison to conventional approaches
Luo et al. Fundamental to achieving fast separations with high efficiency: A review of chromatography with superficially porous particles
Wen et al. “Smart” molecularly imprinted monoliths for the selective capture and easy release of proteins
Alharthi et al. Preparation of mixed-mode stationary phase for separation of peptides and proteins in high performance liquid chromatography
Rakotondravao et al. Characterization of a macroporous epoxy-polymer based resin for the ion-exchange chromatography of therapeutic proteins
Zajickova Review of recent advances in development and applications of organic‐silica hybrid monoliths
Eeltink et al. Recent developments and applications of polymer monolithic stationary phases
Nordborg et al. Monolithic phases for ion chromatography
WO1995010344A1 (en) Beaded capillaries for capillary electrophoresis and chromatographic separations
Kuban et al. Ni (II) functionalized polyhedral oligomeric silsesquioxane based capillary monolith for purification of histidine-tagged proteins by immobilized metal affinity micro-chromatography
Nesterenko Stationary phases for ion separations

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20121212