IN2012DN06243A - - Google Patents
Info
- Publication number
- IN2012DN06243A IN2012DN06243A IN6243DEN2012A IN2012DN06243A IN 2012DN06243 A IN2012DN06243 A IN 2012DN06243A IN 6243DEN2012 A IN6243DEN2012 A IN 6243DEN2012A IN 2012DN06243 A IN2012DN06243 A IN 2012DN06243A
- Authority
- IN
- India
- Prior art keywords
- beads
- binding
- outer shell
- ligands
- buffering
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3291—Characterised by the shape of the carrier, the coating or the obtained coated product
- B01J20/3293—Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28004—Sorbent size or size distribution, e.g. particle size
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
- B01J20/3206—Organic carriers, supports or substrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3244—Non-macromolecular compounds
- B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
- B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/26—Cation exchangers for chromatographic processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to a method of producing novel chromatography media and use thereof for purification ofbiomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially advantageous for production of chroma to focusing media.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE1050157 | 2010-02-19 | ||
PCT/SE2011/050165 WO2011102790A1 (en) | 2010-02-19 | 2011-02-16 | Method for production of chromatography media |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2012DN06243A true IN2012DN06243A (en) | 2015-09-25 |
Family
ID=44483190
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN6243DEN2012 IN2012DN06243A (en) | 2010-02-19 | 2011-02-16 |
Country Status (8)
Country | Link |
---|---|
US (1) | US8574437B2 (en) |
EP (1) | EP2536499A4 (en) |
JP (1) | JP2013520647A (en) |
CN (1) | CN102821843A (en) |
AU (1) | AU2011218487A1 (en) |
CA (1) | CA2789994A1 (en) |
IN (1) | IN2012DN06243A (en) |
WO (1) | WO2011102790A1 (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012005664A1 (en) * | 2010-07-09 | 2012-01-12 | Ge Healthcare Bio-Sciences Ab | Chromatography method and media used therefore |
EP2748181B1 (en) * | 2011-09-30 | 2016-03-30 | GE Healthcare BioProcess R&D AB | Method for purification of cleaved pro-insulin |
CN103418358A (en) * | 2012-05-15 | 2013-12-04 | 北京化工大学 | Chromatography media of composite ligand |
CN106102903A (en) | 2014-03-14 | 2016-11-09 | 通用电气医疗集团生物工艺研发股份公司 | The isolation medium purifying for biologic grain |
US10266563B2 (en) | 2014-06-24 | 2019-04-23 | Ge Healthcare Bio-Process R&D Ab | Chromatography method |
US9537338B2 (en) | 2014-09-16 | 2017-01-03 | Navitas Semiconductor Inc. | Level shift and inverter circuits for GaN devices |
CN104801278A (en) * | 2015-03-29 | 2015-07-29 | 武汉瑞法医疗器械有限公司 | Preparation method of AIDS (acquired immune deficiency syndrome) virus affinity adsorbent |
GB201506117D0 (en) * | 2015-04-10 | 2015-05-27 | Ge Healthcare Bio Sciences Ab | Method for chromatography |
GB201506113D0 (en) * | 2015-04-10 | 2015-05-27 | Ge Healthcare Bio Sciences Ab | Method for chromatography |
CN108883394B (en) * | 2016-04-06 | 2022-05-10 | 思拓凡生物工艺研发有限公司 | Chromatography matrix |
CN109803736B (en) | 2016-09-29 | 2022-02-08 | 生物辐射实验室股份有限公司 | Agarose filled ceramic apatite |
CN108178810B (en) * | 2016-12-07 | 2020-05-19 | 中国科学院大连化学物理研究所 | Preparation and application of reversed phase/anion exchange mixed mode polymer |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5110784A (en) * | 1984-04-09 | 1992-05-05 | Dow Corning Corporation | Dual surface porous material |
US5135650A (en) * | 1988-12-22 | 1992-08-04 | Bio-Rad Laboratories, Inc. | Chromatography stationary phase material for high performance liquid chromatography |
US20040214157A1 (en) * | 1994-06-29 | 2004-10-28 | Simon C. Burton | Chromatographic resins and methods for using same |
SE9700383D0 (en) | 1997-02-04 | 1997-02-04 | Pharmacia Biotech Ab | An adsorption / separation method and a medium for adsorption / separation |
SE9700769D0 (en) * | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matrices for separation and separation that utilize the matrices |
ES2267840T3 (en) * | 2000-12-31 | 2007-03-16 | Ge Healthcare Bio-Sciences Ab | METHOD FOR MANUFACTURING COMPOSITIONS THAT CONTAIN LOW SALT CONCENTRATIONS. |
US20040016702A1 (en) * | 2002-07-26 | 2004-01-29 | Applera Corporation | Device and method for purification of nucleic acids |
SE0202551D0 (en) * | 2002-08-27 | 2002-08-27 | Amersham Biosciences Ab | Chromatographic two-layer particles |
SG131016A1 (en) * | 2005-09-19 | 2007-04-26 | Millipore Corp | Asymmetric porous adsorptive bead |
US20110045574A1 (en) * | 2008-04-22 | 2011-02-24 | Jan Bergstrom | Chromatography medium |
WO2010005364A1 (en) * | 2008-07-08 | 2010-01-14 | Ge Healthcare Bio-Sciences Ab | Separation medium for chromatography of various biomolecules |
GB0814326D0 (en) * | 2008-08-05 | 2008-09-10 | Medical Res Council | Phosphate binding materials and their uses |
-
2011
- 2011-02-16 JP JP2012553845A patent/JP2013520647A/en not_active Withdrawn
- 2011-02-16 EP EP11744970.2A patent/EP2536499A4/en not_active Withdrawn
- 2011-02-16 US US13/575,750 patent/US8574437B2/en not_active Expired - Fee Related
- 2011-02-16 AU AU2011218487A patent/AU2011218487A1/en not_active Abandoned
- 2011-02-16 IN IN6243DEN2012 patent/IN2012DN06243A/en unknown
- 2011-02-16 CA CA2789994A patent/CA2789994A1/en not_active Abandoned
- 2011-02-16 CN CN2011800101971A patent/CN102821843A/en active Pending
- 2011-02-16 WO PCT/SE2011/050165 patent/WO2011102790A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
CA2789994A1 (en) | 2011-08-25 |
EP2536499A4 (en) | 2013-08-14 |
AU2011218487A1 (en) | 2012-09-06 |
US8574437B2 (en) | 2013-11-05 |
CN102821843A (en) | 2012-12-12 |
EP2536499A1 (en) | 2012-12-26 |
JP2013520647A (en) | 2013-06-06 |
WO2011102790A1 (en) | 2011-08-25 |
US20120309618A1 (en) | 2012-12-06 |
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