SG10201901695RA - Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers - Google Patents

Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers

Info

Publication number
SG10201901695RA
SG10201901695RA SG10201901695RA SG10201901695RA SG10201901695RA SG 10201901695R A SG10201901695R A SG 10201901695RA SG 10201901695R A SG10201901695R A SG 10201901695RA SG 10201901695R A SG10201901695R A SG 10201901695RA SG 10201901695R A SG10201901695R A SG 10201901695RA
Authority
SG
Singapore
Prior art keywords
elution
mops
biomolecules
mes
mobile phase
Prior art date
Application number
SG10201901695RA
Inventor
Rahul Godawat
Daniel Cummings
Veena Warikoo
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of SG10201901695RA publication Critical patent/SG10201901695RA/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
    • A61B50/30—Containers specially adapted for packaging, protecting, dispensing, collecting or disposing of surgical or diagnostic appliances or instruments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/14—Details; Accessories therefor
    • A61J1/16—Holders for containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01022—Alpha-galactosidase (3.2.1.22)

Abstract

ELUTION OF BIOMOLECULES FROM MULTI-MODAL RESINS USING MES AND MOPS AS MOBILE PHASE MODIFIERS The present invention relates to methods for purifying or enriching a biomolecule using multimodal resins and an elution buffer containing a Good's buffer. [Figure 1]
SG10201901695RA 2012-10-24 2013-10-23 Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers SG10201901695RA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261717880P 2012-10-24 2012-10-24

Publications (1)

Publication Number Publication Date
SG10201901695RA true SG10201901695RA (en) 2019-03-28

Family

ID=49554488

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201502918RA SG11201502918RA (en) 2012-10-24 2013-10-23 Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers
SG10201901695RA SG10201901695RA (en) 2012-10-24 2013-10-23 Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201502918RA SG11201502918RA (en) 2012-10-24 2013-10-23 Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers

Country Status (9)

Country Link
US (2) US20150275195A1 (en)
EP (1) EP2911759A1 (en)
CN (3) CN105777862A (en)
AU (3) AU2013334644B2 (en)
CA (1) CA2888824C (en)
HK (1) HK1222868A1 (en)
MX (1) MX2015005313A (en)
SG (2) SG11201502918RA (en)
WO (1) WO2014066471A1 (en)

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SG11201502918RA (en) * 2012-10-24 2015-05-28 Genzyme Corp Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers
RU2662929C2 (en) * 2013-03-08 2018-07-31 Джензим Корпорейшн Integrated continuous manufacturing of therapeutic protein drug substances
TWI709570B (en) 2014-01-17 2020-11-11 美商健臻公司 Sterile chromatography and manufacturing processes
TWI709569B (en) 2014-01-17 2020-11-11 美商健臻公司 Sterile chromatography resin and use thereof in manufacturing processes
CA3084827A1 (en) * 2017-12-29 2019-07-04 Baxalta Incorporated Adeno-associated virus purification methods
CN108479110A (en) * 2018-04-10 2018-09-04 深圳市菲鹏生物制药股份有限公司 A kind of cell culture liquid decolorizer and discoloration method
EP3843869A1 (en) 2018-08-31 2021-07-07 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
DE102020214647A1 (en) 2020-11-20 2022-05-25 DIL Deutsches Institut für Lebensmitteltechnik e.V. Anthocyanin-containing extract powder and method of manufacture

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US4347322A (en) * 1981-01-12 1982-08-31 Nabisco Brands, Inc. Chromatographic process for enzyme purification
US4663288A (en) * 1985-05-22 1987-05-05 Nabisco Brands, Inc. Process for purification of enzymes
MX340738B (en) 1996-09-13 2016-07-22 Shire Human Genetic Therapies THERAPY FOR α-GALACTOSIDASE A DEFICIENCY.
WO2006105426A2 (en) * 2005-03-30 2006-10-05 Neose Technologies, Inc. Manufacturing process for the production of peptides grown in insect cell lines
US20080090278A1 (en) * 2006-03-31 2008-04-17 Toyo Boseki Kabushiki Kaisha Method for enhancing stability of a composition comprising soluble glucose dehydrogenase (gdh)
ATE503763T1 (en) 2007-07-11 2011-04-15 Novo Nordisk As PURIFICATION OF FACTOR VIII USING A MIXED-MODE OR MULTIMODAL RESIN
WO2009092010A1 (en) * 2008-01-18 2009-07-23 Gagnon Peter S Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography
EP2537862B1 (en) 2008-06-24 2015-03-18 Octapharma AG A process of purifying coagulation factor VIII
WO2010030222A1 (en) 2008-09-12 2010-03-18 Ge Healthcare Bio-Sciences Ab Enhanced protein aggregate removal with multimodal anion exchangers in the presence of protein-excluded zwitterions
CA2697999C (en) * 2009-04-24 2013-04-30 Werner Hoelke A stabilized aqueous alpha-galactosidase composition and methods relating thereto
US20120208986A1 (en) * 2009-10-20 2012-08-16 Wenger Marc D Use of mixed mode chromatography for the capture and purification of basic antibody products
WO2011088225A1 (en) * 2010-01-15 2011-07-21 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
WO2011121020A1 (en) 2010-03-30 2011-10-06 Octapharma Ag A process for purifying vitamin k dependent proteins such as coagulation factor ix
CN105859870A (en) * 2010-03-30 2016-08-17 奥克塔法马股份有限公司 Process for the purification of growth factor protein
US9422329B2 (en) * 2010-11-05 2016-08-23 Hoffmann-La Roche Inc. Optimized method for antibody capturing by mixed mode chromatography
WO2012062753A1 (en) * 2010-11-09 2012-05-18 Qiagen Gmbh Method and device for isolating and purifying double-stranded nucleic acids
WO2012173876A1 (en) * 2011-06-13 2012-12-20 Merck Sharp & Dohme Corp. Methods of purification of native or mutant forms of diphtheria toxin
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SG11201502918RA (en) * 2012-10-24 2015-05-28 Genzyme Corp Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers
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Also Published As

Publication number Publication date
CN104884147A (en) 2015-09-02
AU2020203008B2 (en) 2022-06-16
AU2018241214A1 (en) 2018-11-01
CN111172135A (en) 2020-05-19
SG11201502918RA (en) 2015-05-28
CN105777862A (en) 2016-07-20
HK1222868A1 (en) 2017-07-14
CA2888824C (en) 2021-02-02
US20200131493A1 (en) 2020-04-30
MX2015005313A (en) 2016-04-06
AU2018241214B2 (en) 2020-02-06
AU2013334644A1 (en) 2015-05-07
WO2014066471A1 (en) 2014-05-01
CA2888824A1 (en) 2014-05-01
AU2018241214B9 (en) 2020-02-20
US20150275195A1 (en) 2015-10-01
AU2020203008A1 (en) 2020-05-28
EP2911759A1 (en) 2015-09-02
AU2013334644B2 (en) 2018-07-12
US11203747B2 (en) 2021-12-21

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