CN102793640B - Vitiligo covering composition and preparation method thereof - Google Patents
Vitiligo covering composition and preparation method thereof Download PDFInfo
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- CN102793640B CN102793640B CN 201210303530 CN201210303530A CN102793640B CN 102793640 B CN102793640 B CN 102793640B CN 201210303530 CN201210303530 CN 201210303530 CN 201210303530 A CN201210303530 A CN 201210303530A CN 102793640 B CN102793640 B CN 102793640B
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- Medicines Containing Plant Substances (AREA)
Abstract
A deep-sea seaweed extract having a vitiligo covering effect is characterized in that: raw materials of the extract include 60-80 parts of blue algae, 40-60 parts of green algae, 25-40 parts of brown algae, 15-30 parts of red algae, 12-26 parts of fucus, and 5-15 parts of beets; and the preparation method includes extracting a crude extract A by ultrasonic extraction, then preparing a fine extract B by using a supercritical CO2 fluid extraction method, and obtaining white powder of the deep-sea algae extract after recrystallization. Daubing the deep-sea seaweed extract of the invention by external use, a color similar to the color of skin can be quickly formed on white spots to cover the white spots, the skin is colored safely, and the effect is naturally beautiful, very uniform and lasting. Under normal circumstances, the coloring can last for 5-15 days.
Description
Technical field
The invention belongs to the cosmetic field that vitiligo hides, be specifically related to a kind of vitiligo and hide composition and method of making the same, this vitiligo covering compositions has the white macula of covering and beautifies skin function, and it is with respect to existing covering agent, painted fast, painted lasting, and effect is very natural.
Background technology
Vitiligo is a kind of common multiple pigmented dermatosis.It is feature that this disease forms white macula with local or general property depigmentation, is a kind of acquired limitation or general property depigmentation of skin, is the common skin diseases of an influence beauty treatment, easily diagnosis, and treatment is difficult.To be posteriority lose the white patch of the limitation that takes place because skin pigment takes off to vitiligo, makes local skin be the white macula sample, medically usually this pathological changes depigmentation.Vitiligo all has generation all over the world, China has ten million people morbidity approximately, and sickness rate is about 1%-2%, is one of world's dermatosis three big pertinacious diseases.
At present, the therapy for vitiligo method is varied, comprising Western medicine therapy, herbal treatment, the combined therapy of Chinese and Western medicine and physical therapy therapy etc.Yet because leucoderma disease is because of complexity, its pathogeny is clear and definite fully as yet at present, therefore, treats quite difficulty of vitiligo effectively, and this has greatly influenced the life quality of patients with vitiligo.
In numerous therapy of vitiligo schemes, the most frequently used method comprises that oral Fructus Psoraleae is in conjunction with the therapy of ultraviolet radiation.For example, people such as Bian Ertang, " Chinese medicine adds long wave ultraviolet therapy vitiligo 70 examples and analyzes ", " practical Chinese medicine magazine ", the 3rd phase of nineteen ninety-five discloses the Therapeutic Method of oral Chinese medicine in conjunction with the irradiation long wave ultraviolet, and prescriptions of Chinese medicine wherein is the Radix Astragali, the Radix Angelicae Dahuricae, Fructus Psoraleae, Radix Polygoni Multiflori Preparata Radix Salviae Miltiorrhizae.Liu Cuijie etc., " vifiligo pill is united the leukodermic observation of curative effect of narrow spectrum ultraviolet B radiation irradiation treatment " " " 2008 national combination of Chinese and Western medicine skin cypridology academic meeting paper compilation ", also disclose oral vifiligo pill in conjunction with the Therapeutic Method of ultraviolet B radiation, the main component of vifiligo pill wherein is Fructus Psoraleae, the Radix Astragali, Flos Carthami, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi, Semen Persicae, Radix Salviae Miltiorrhizae, Zaocys, Radix Arnebiae (Radix Lithospermi), Cortex Dictamni, Rhizoma Dioscoreae, Rhizoma Zingiberis, Radix Gentianae and Fructus Tribuli.Though above-mentioned Therapeutic Method has certain effect, but it has all utilized ultraviolet, and well-knownly be, the vitiligo affected part lacks the protection system of normal skin, very low for ultraviolet defensive ability/resistance ability, therefore, contact ultraviolet for a long time and not only can not play good therapeutic effect, may increase the damage to skin on the contrary, severe patient even may cause skin carcinoma.
Occur with head, face, neck and limb end because leukodermic affected part is many, therefore, dressing is difficult to hide patient's white macula, and this has greatly influenced the daily routines of patients with vitiligo.In the last few years, utilized dihydroxyacetone and skin can form artificial brown's principle, domestic-developed numerous treatments or hide leukodermic medicine or cosmetic composition.99114078.8 disclose a kind of externally-applied medicinal composition, its ethanol extraction and laurocapram by mustine hydrochlcride, dihydroxyacetone, Fructus Psoraleae Flos Carthami is formed.200410070380.2 disclose a kind of leukoderma covering agent of long-lasting water proof and wearable, it is made up of food coloring, essence, dihydroxyacetone, ethanol and water.200510002653.4 disclose a kind of skin pigment reduction external used medicine for the treatment of, comprised dihydroxyacetone, vinegar, ethanol, water and essence.200610137625.8 disclose a kind of skin pigment reduction external used medicine for the treatment of equally, it is made by ethanol, Radix Polygoni Multiflori, Fructus Psoraleae, the Flos Sophorae Immaturus, dihydroxyacetone.
Though the leukodermic effect of covering of can both playing to a certain extent of above-mentioned therapy of vitiligo or covering compositions; but; because dihydroxyacetone and skin keratin effect need 8 hours time; therefore; patients with vitiligo often need use in night; could form at second day stable painted, thereby do not influence its work or daily routines, this has greatly influenced ease of use.Secondly, dead skin cells in dihydroxyacetone and the keratodermatitis interacts and forms brown is a kind of of short duration coloring reaction, general this color can not continue to surpass 3-5 days time, along with painted variation, tangible contrast can appear in the color of affected part color and normal skin, and needs of patients is smeared Masking sol adjustment again.
Secondly, the unstability of dihydroxyacetone in aqueous solution restricted the development that contains the dihydroxyacetone compositions, and dihydroxyacetone is very sensitive to light, heat, humidity, air and alkaline pH value.As time goes on, the color of dihydroxyacetone goods can be deepened gradually, and be accompanied by a kind of strange taste, make that user is difficult to accept, and hiding leukodermic effect is not remarkable especially, and the aberration that is difficult to remedy is arranged, and be difficult to painted hot summer, the coloring effect instability also has a lot of people that allergic phenomena is arranged.More than all development and application that all seriously limits this class covering agent.
Therefore, seek a kind of both safety, stable again to have very good effect and painted lasting vitiligo to hide product be the urgent demand that faces at present.
Summary of the invention
In order to overcome the deficiencies in the prior art, the inventor is through hiding discovering of product to vitiligo for many years, the active substance that extracts from some deep-sea Sargassum has surprising effect aspect the vitiligo covering, after external is applied to the vitiligo affected part, not only can form the color close with the colour of skin fast at the white macula place and then hide white macula, and dye safety, effect is natural beauty more, very evenly and lasting, generally speaking, paintedly can continue 5-15 days.The inventor also further finds, some active factors in the extract also has preserves moisture, skin moistening, reparation skin, improves the effect of oils and fats distribution etc., and therefore, life-time service product of the present invention has certain skin metabolism and improves function.Because this extract is pure natural substance to be extracted and to form, it is as safe as a house, without any toxic and side effects.
Therefore, an object of the present invention is to provide a kind of deep-sea Sargassum extract with vitiligo covering effect, the raw material of this extract is: cyanophyceae 60-80 part, chlorella 40-60 part, Brown algae 25-40 part, red algae 15-30 part, Fucus Vesiculosus 12-26 part, Radix Betae 5-15 part.This preparation method of extract is for obtaining crude extract A with ultrasonic extraction earlier, utilize the supercritical carbon dioxide extraction legal system to get extract B then, obtain the extract that the white crystalline powder mixture is the application through recrystallization again, we are referred to as " the shining skin element of ACS algae alive ".
Another purpose of the application provides a kind of preparation method with deep-sea Sargassum extract of vitiligo covering effect, and its concrete preparation process is:
1) take by weighing raw material cyanophyceae 60-80 part, chlorella 40-60 part, Brown algae 25-40 part, red algae 15-30 part, Fucus Vesiculosus 12-26 part, Radix Betae 5-15 part respectively by formula ratio, standby;
2) raw material with step 1) joins in the extraction pot, and adds the water of 10 to 15 times of weight of raw material, regulates pH value 3-4 and soaks after 24 hours, boiled 5 to 8 hours, natural cooling carries out continuous ultrasound then and extracted 6 to 12 hours and filtered, and concentrated extracting solution obtains crude extract A;
3) utilize the supercritical carbon dioxide extraction technology that crude extract A is further extracted, wherein Cui Qu temperature is 35-55 ℃, and pressure is 8-30MP, obtains extract B after 10 hours;
4) at 35-45 ℃, stir and down extract B to be dissolved in 95% the ethanol, when crude extract B dissolves fully, move to rapidly and lower the temperature in the ice-water bath, separate out white powder, be deep-sea of the present invention Sargassum extract (ACS live the shining skin element of algae).
Another purpose of the application provides a kind of compositions with vitiligo covering effect, it is characterized in that said composition comprises deep-sea of the present invention Sargassum extract, water and cosmetics or pharmaceutically acceptable carrier.
Common cosmetics and the example of pharmaceutically acceptable carrier include but not limited to: Percutaneous absorption enhancer, solubilizing agent, cosolvent, antioxidant, chelating agen, antiseptic, surfactant, base oil, flavouring agent, pigment etc. or their combination.
The compositions that the present invention has vitiligo covering effect can be the common liquid in this area or ointment form, for example, and solution, Emulsion, ointment etc.
Description of drawings
Fig. 1-2 uses the effect contrast figure of compositions for the back of the hand portion
Fig. 3-4 uses the effect contrast figure of compositions for cervical region
Fig. 5-7 does not use compositions respectively the time, uses the effect contrast figure after 3 hours and 15 days
The specific embodiment
Extract embodiment 1
1) take by weighing 60 parts of cyanophyceaes, 40 parts of chlorellas, 25 parts of Brown algaes, 15 parts of red algaes, 12 parts of Fucus Vesiculosus, 5 parts of Radix Betaes, standby;
2) raw material with step 1) joins in the extraction pot, and adds the water of 10 times of weight of raw material, regulates pH value 3 and soaks after 24 hours, boils 5 hours, and natural cooling carried out supersound extraction 6 hours and filtration then, and concentrated extracting solution obtains crude extract A;
3) utilize the supercritical carbon dioxide extraction technology that crude extract A is further extracted, wherein Cui Qu temperature is 35 ℃, and pressure is 8MP, obtains extract B;
4) at 35 ℃, stir and down extract B to be dissolved in 95% the ethanol, when crude extract B dissolves fully, move to rapidly and lower the temperature in the ice-water bath, separate out white powder, be deep-sea of the present invention Sargassum extract.
Extract embodiment 2
1) take by weighing 65 parts of raw material cyanophyceaes, 48 parts of chlorellas, 26 parts of Brown algaes, 18 parts of red algaes, 14 parts of Fucus Vesiculosus, 7 parts of Radix Betaes respectively by formula ratio, standby;
2) raw material with step 1) joins in the extraction pot, and adds the water of 10 times of weight of raw material, regulates pH value 3 and soaks after 24 hours, boils 5 hours, and natural cooling carried out supersound extraction 8 hours and filtration then, and concentrated extracting solution obtains crude extract A;
3) utilize the supercritical carbon dioxide extraction technology that crude extract A is further extracted, wherein Cui Qu temperature is 40 ℃, and pressure is 8MP, obtains extract B;
4) at 36 ℃, stir and down extract B to be dissolved in 95% the ethanol, when crude extract B dissolves fully, move to rapidly and lower the temperature in the ice-water bath, separate out white powder, be deep-sea of the present invention Sargassum extract.
Extract embodiment 3
1) take by weighing 70 parts of raw material cyanophyceaes, 55 parts of chlorellas, 30 parts of Brown algaes, 25 parts of red algaes, 18 parts of Fucus Vesiculosus, 9 parts of Radix Betaes respectively by formula ratio, standby;
2) raw material with step 1) joins in the extraction pot, and adds the water of 14 times of weight of raw material, regulates pH value 4 and soaks after 24 hours, boils 7 hours, and natural cooling carried out supersound extraction 10 hours and filtration then, and concentrated extracting solution obtains crude extract A;
3) utilize the supercritical carbon dioxide extraction technology that crude extract A is further extracted, wherein Cui Qu temperature is 45 ℃, and pressure is 25MP, obtains extract B;
4) at 38 ℃, stir and down extract B to be dissolved in 95% the ethanol, when crude extract B dissolves fully, move to rapidly and lower the temperature in the ice-water bath, separate out white powder, be deep-sea of the present invention Sargassum extract.
Extract embodiment 4
1) take by weighing 75 parts of raw material cyanophyceaes, 58 parts of chlorellas, 35 parts of Brown algaes, 27 parts of red algaes, 22 parts of Fucus Vesiculosus, 12 parts of Radix Betaes respectively by formula ratio, standby;
2) raw material with step 1) joins in the extraction pot, and adds the water of 14 times of weight of raw material, regulates pH value 4 and soaks after 24 hours, boils 8 hours, and natural cooling carried out supersound extraction 12 hours and filtration then, and concentrated extracting solution obtains crude extract A;
3) utilize the supercritical carbon dioxide extraction technology that crude extract A is further extracted, wherein Cui Qu temperature is 50 ℃, and pressure is 30MP, obtains extract B;
4) at 40 ℃, stir and down extract B to be dissolved in 95% the ethanol, when crude extract B dissolves fully, move to rapidly and lower the temperature in the ice-water bath, separate out white powder, be deep-sea of the present invention Sargassum extract.
Extract embodiment 6
1) take by weighing 80 parts of raw material cyanophyceaes, 60 parts of chlorellas, 40 parts of Brown algaes, 30 parts of red algaes, 26 parts of Fucus Vesiculosus, 15 parts of Radix Betaes respectively by formula ratio, standby;
2) raw material with step 1) joins in the extraction pot, and adds the water of 15 times of weight of raw material, regulates pH value 4 and soaks after 24 hours, boils 8 hours, and natural cooling carried out supersound extraction 11 hours and filtration then, and concentrated extracting solution obtains crude extract A;
3) utilize the supercritical carbon dioxide extraction technology that crude extract A is further extracted, wherein Cui Qu temperature is 55 ℃, and pressure is 30MP, obtains extract B;
4) at 45 ℃, stir and down extract B to be dissolved in 95% the ethanol, when crude extract B dissolves fully, move to rapidly and lower the temperature in the ice-water bath, separate out white powder, be deep-sea of the present invention Sargassum extract.
Embodiment 7
The extract of embodiment 1 is mixed according to 1: 5 ratio of weight ratio with water, obtain the aqueous solution of deep-sea Sargassum extract.
Embodiment 8
Extract, water, the Fructus Citri Limoniae essence of embodiment 1 are mixed according to 1: 8: 0.01 ratio of weight ratio, obtain the compositions of deep-sea Sargassum extract.
Embodiment 9
Extract, water, the ascorbic acid of embodiment 2 are mixed according to 1: 6: 0.02 ratio of weight ratio, obtain the compositions of deep-sea Sargassum extract.
Embodiment 10
With the extract of embodiment 5, water, dehydrated alcohol, EDTA according to weight ratio 1: 4: 0.5: 0.03 ratio is mixed, and obtains the compositions of deep-sea Sargassum extract.
Embodiment 11
With the extract of embodiment 6, water, methyl parahydroxybenzoate, flavoring orange essence, sodium thiosulfate according to weight ratio 1: 8: 0.01: 0.02: 0.03 ratio is mixed, and obtains the compositions of deep-sea Sargassum extract.
Be effect and the experiment of local irritation safety evaluatio and the data of compositions of the present invention below:
Experiment one: local excitation test
Adopt 20 of mices, back unhairing area 2cmX2cm.Be divided into 2 groups at random: the aqueous solution group of blank group, embodiment 7,10 every group.Smear the water of equivalent and the aqueous solution of embodiment 7, every day 2 times, continuous 30 days respectively in 2 groups of mice depilation districts.During this period, each organizes mice depilation in per 3 days once.Experimental result shows that blank group mouse skin hair color is constant, and uses the mice of the aqueous solution of embodiment 7 to begin to deepen smearing 2 hours skin colors, and color tends towards stability and is dark brown after using 3 days.When 30 day experimental period finished, having any different in two groups of mouse test positions, red, any dermal toxicity effect such as swell, come out in a rash does not appear all on color.
Test two: coloring effect test
Get 0.5 milliliter of the aqueous solution of the embodiment of the invention 7, smear evenly with the fine, soft fur pen in hand, cervical region and the shirtfront of patients with vitiligo respectively, after smearing 3 hours, observe the dye effect, the result shows, all form the color close with skin at hand, cervical region and anterior part of chest, concrete effect is referring to accompanying drawing 1-4.
Experiment three: painted endurance life test
Smear the aqueous solution of embodiment 7 at patient's arm, observe respectively and smear change color preceding, after 3 hours and 15 days, concrete effect is referring to accompanying drawing 5-7.
Experiment four: stability test
The aqueous solution of getting embodiment 7 is packed in the open glass bottle, subsequently the open glass bottle is positioned in the constant temperature humidity chamber, adjust the temperature to 55 degree, humidity is 75% to carry out accelerated stability test, after 3 months, 6 months, 1 year, take out the variation of observing solution and carry out staining test.Experimental result shows, behind the accelerated stability test solution aspect color, clarity, abnormal smells from the patient and coloring effect without any variation.
Claims (6)
1. deep-sea Sargassum extract with vitiligo covering effect, the raw material that it is characterized in that this extract is: cyanophyceae 60-80 part, chlorella 40-60 part, Brown algae 25-40 part, red algae 15-30 part, Fucus Vesiculosus 12-26 part, Radix Betae 5-15 part, its preparation method is for obtaining crude extract A with ultrasonic extraction earlier, utilize the supercritical carbon dioxide extraction legal system to get extract B then, namely obtain the deep-sea Sargassum extract of white powder again through recrystallization, its concrete preparation method is:
1) take by weighing raw material cyanophyceae 60-80 part, chlorella 40-60 part, Brown algae 25-40 part, red algae 15-30 part, Fucus Vesiculosus 12-26 part, Radix Betae 5-15 part respectively by formula ratio, standby;
2) raw material with step 1) joins in the extraction pot, and adds the water of 10 to 15 times of weight of raw material, regulates pH value 3-4 and soaks after 24 hours, boiled 5 to 8 hours, natural cooling carries out continuous ultrasound then and extracted 6 to 12 hours and filtered, and concentrated extracting solution obtains crude extract A;
3) utilize the supercritical carbon dioxide extraction technology that crude extract A is further extracted, wherein Cui Qu temperature is 35-55 ℃, and pressure is 8-30MP, obtains extract B after 10 hours;
4) at 35-45 ℃, stir and down extract B to be dissolved in 95% the ethanol, when crude extract B dissolves fully, move to rapidly and lower the temperature in the ice-water bath, separate out white powder, be described deep-sea Sargassum extract.
2. one kind prepares the described method with deep-sea Sargassum extract of vitiligo covering effect of claim 1, it is characterized in that comprising following step:
1) take by weighing raw material cyanophyceae 60-80 part, chlorella 40-60 part, Brown algae 25-40 part, red algae 15-30 part, Fucus Vesiculosus 12-26 part, Radix Betae 5-15 part respectively by formula ratio, standby;
2) raw material with step 1) joins in the extraction pot, and adds the water of 10 to 15 times of weight of raw material, regulates pH value 3-4 and soaks after 24 hours, boiled 5 to 8 hours, natural cooling carries out continuous ultrasound then and extracted 6 to 12 hours and filtered, and concentrated extracting solution obtains crude extract A;
3) utilize the supercritical carbon dioxide extraction technology that crude extract A is further extracted, wherein Cui Qu temperature is 35-55 ℃, and pressure is 8-30MP, obtains extract B after 10 hours;
4) at 35-45 ℃, stir and down extract B to be dissolved in 95% the ethanol, when crude extract B dissolves fully, move to rapidly and lower the temperature in the ice-water bath, separate out white powder, be described deep-sea Sargassum extract.
3. compositions with vitiligo covering effect is characterized in that comprising the deep-sea Sargassum extract of claim 1.
4. the compositions with vitiligo covering effect according to claim 3 is characterized in that further comprising water and cosmetics or pharmaceutically acceptable carrier.
5. the compositions with vitiligo covering effect according to claim 4 is characterized in that cosmetics and pharmaceutically acceptable carrier are Percutaneous absorption enhancer, solubilizing agent, cosolvent, antioxidant, chelating agen, antiseptic, surfactant, base oil, flavouring agent, pigment or their combination.
6. the compositions with vitiligo covering effect according to claim 5 is characterized in that said composition is solution, Emulsion or ointment form.
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| CN105168051A (en) * | 2015-09-16 | 2015-12-23 | 任海锋 | Skin color coverage foundation |
| CN105250166B (en) * | 2015-09-25 | 2018-08-31 | 北京盖百霖白癜风遮盖医学研究院 | It is a kind of to cover more beautiful, natural leucoderma covering composition |
| CN109771317A (en) * | 2019-03-08 | 2019-05-21 | 唐凯 | A kind of covering composition of leucoderma and scar hickie |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006126675A1 (en) * | 2005-05-27 | 2006-11-30 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Agent for external application to the skin |
| CN102078279A (en) * | 2010-12-28 | 2011-06-01 | 海南京润珍珠生物技术股份有限公司 | BB Cream (Blemish Balm Cream) added with dense silica-coated pearl powder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006126675A1 (en) * | 2005-05-27 | 2006-11-30 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Agent for external application to the skin |
| CN102078279A (en) * | 2010-12-28 | 2011-06-01 | 海南京润珍珠生物技术股份有限公司 | BB Cream (Blemish Balm Cream) added with dense silica-coated pearl powder |
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| Title |
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| 海藻多糖的结构、提取和生物活性研究新进展;罗先群等;《中国食品添加剂》;20061231(第4期);第100页至第105页 * |
| 罗先群等.海藻多糖的结构、提取和生物活性研究新进展.《中国食品添加剂》.2006,(第4期), |
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Effective date of registration: 20170605 Address after: 100068, 106, Fengtai District, Majiabao East Road, Beijing ocean new natural 720 room Patentee after: Beijing three li Biotechnology Co., Ltd. Address before: 100068, 106, Fengtai District, Majiabao East Road, Beijing ocean new natural 720 room Patentee before: Hu Fagang |