CN102793122A - Method of removing mildew of molded bamboo shoot heart - Google Patents

Method of removing mildew of molded bamboo shoot heart Download PDF

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Publication number
CN102793122A
CN102793122A CN2012103140423A CN201210314042A CN102793122A CN 102793122 A CN102793122 A CN 102793122A CN 2012103140423 A CN2012103140423 A CN 2012103140423A CN 201210314042 A CN201210314042 A CN 201210314042A CN 102793122 A CN102793122 A CN 102793122A
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CN
China
Prior art keywords
bamboo shoot
mildew
fermentation
stage
mould
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CN2012103140423A
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Chinese (zh)
Inventor
金德保
齐永玲
王力生
陈芳
李大威
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NINGGUO XIANGWEIYUAN AGRICULTURE PRODUCT DEVELOPMENT Co Ltd
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NINGGUO XIANGWEIYUAN AGRICULTURE PRODUCT DEVELOPMENT Co Ltd
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Priority to CN2012103140423A priority Critical patent/CN102793122A/en
Publication of CN102793122A publication Critical patent/CN102793122A/en
Pending legal-status Critical Current

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Abstract

The invention belongs to the technical field of mildew removal, and provides a method of removing mildew of a molded bamboo shoot heart. The method adopts a technology of mixed bacteria asynchronous raw material fermentation to remove and prevent the mildew, the technology is divided into an aerobic fermentation stage and an anaerobic fermentation stage, and by utilizing microorganism fermentation of the bamboo shoot heart, the method has a positive effect on inhibiting the content of mould of the bamboo shoot heart, in particular on degrading aflatoxin B1; the microorganism fermentation manner not only has a mildew-proof capacity, which is same to a mildew preventive, but can be used for degrading the original toxin in the bamboo shoot heart, so that the method has more advantages on mildew prevention than the mildew preventive; through microorganism fermentation, mould growth is inhibited, mycotoxin is degraded, conditions of nutrition damage and secondary pollution caused by general treatment methods of high temperature, strong acid, strong alkali and absorbents at the present stage can be avoided, the mycotoxin is removed safely, toxic and harmful chemical substances are not brought, meanwhile, loss of nutrient substances in the bamboo shoot hearth can be reduced as much as possible, and the method has the advantages of stronger popularization and application values.

Description

A kind of bamboo shoot pocket that goes mouldy goes mould method
Technical field
The invention belongs to mould technical field, relate in particular to a kind of bamboo shoot pocket that goes mouldy and go mould method.
Background technology
Because the bamboo shoot pocket concentrates listing, water content big, nutritious, adds local hot and humid environment etc., the bamboo shoot pocket is very easily mouldy to rot, and utilizes very difficulty, becomes the major issue of puzzlement local environment.
It is reported; Yeast has direct degradation to mycotoxin during the fermentation, and Hom BW (1995) adds a certain amount of yeast culture in containing the feed of aflatoxin; 88% aflatoxin is degraded; The cell wall constituent of yeast or yeast can be used as mold toxin sorbent and uses, and bacillus subtilis can degrading aflatoxin B 1, suppresses the aspergillus flavus growth.
Present stage, the interpolation mould inhibitor that extensively adopts can mildew-resistant, but the toxin that exists originally in the bamboo shoot pocket of can not degrading; Processing methods such as high temperature, strong acid, highly basic, adsorbent can cause nutrition destruction and secondary pollution, can not reach the purpose that safety is removed mycotoxin.
Summary of the invention
The invention provides a kind of bamboo shoot pocket that goes mouldy and go mould method, be intended to solution, add local hot and humid environment owing to the concentrated listing of bamboo shoot pocket, water content are big, nutritious; The bamboo shoot pocket is very easily mouldy to rot; Environmental pollution is comparatively serious, but the extensively interpolation mould inhibitor mildew-resistant of employing of present stage simultaneously, but the toxin that exists originally in the bamboo shoot pocket of can not degrading; Processing methods such as high temperature, strong acid, highly basic, adsorbent can cause nutrition destruction and secondary pollution, can not reach the problem that safety is removed the mycotoxin purpose.
The object of the present invention is to provide a kind of bamboo shoot pocket that goes mouldy to go mould method, this method may further comprise the steps:
54g is cut up with a hay cutter broken mouldy bamboo shoot pocket mix with the 6g wheat bran, the glucose that adds 0.6g is as carbon source, and the urea of 0.9g is as nitrogenous source; Under the condition of nature pH, inoculation yeast bacterium 2%-8%, bacillus subtilis 2%-8%; 35 ℃ of fermentation 24h-48h, this stage is called the aerobic fermentation stage;
The glucose, the urea of 0.9g and the plant lactobacillus liquid of 2%-8% that add 0.6g, with carbon dioxide emptying air, 35 ℃ of fermentation 24h-48h, this stage is called the anaerobic fermentation stage.
Further, mould bacterial content is 26 * 10 in the broken mouldy bamboo shoot pocket of 54g hand hay cutter 2Cfu/g, AFB 1Content is 3.20ug/g.
The bamboo shoot pocket that goes mouldy provided by the invention goes mould method; Adopt the asynchronous raw material fermentation of a kind of Mixed Microbes to go the technology of mould and mildew-resistant; At first carry out the aerobic fermentation stage: 54g is cut up with a hay cutter broken mouldy bamboo shoot pocket and 6g wheat bran put into container, the glucose that adds 0.6g is as carbon source, and the urea of 0.9g is as nitrogenous source; Inoculation yeast bacterium 2%-8% under the nature pH condition, bacillus subtilis 2%-8%; 35 ℃ of ferment 24h or 48h; Get into the anaerobic fermentation stage then, add the glucose of 0.6g, the plant lactobacillus liquid of the urea of 0.9g and 2%-8%, with carbon dioxide emptying air, 35 ℃ of fermentation 24h-48h; This method utilizes microbial fermentation bamboo shoot pocket for suppressing bamboo shoot pocket mould bacterial content, particularly aflatoxin degradation B 1Positive effect is arranged; Adopt the mode of microbial fermentation not only to have the mildew-resistant ability identical with mould inhibitor, and the toxin that exists originally in the bamboo shoot pocket of can degrading, more have superiority than adding the mould inhibitor mildew-resistant; Come mould fungus inhibition growth and degraded mycotoxin through microbial fermentation; Can avoid the nutrition destruction that the processing method of high temperature that present stage extensively adopts, strong acid, highly basic, adsorbent causes and the situation of secondary pollution; Reach safety and remove the purpose of mycotoxin, in this process, need not to bring into poisonous and hazardous chemical substance; Also reduce the loss of nutriment in the bamboo shoot pocket simultaneously as much as possible, have stronger popularization and using value.
Description of drawings
Fig. 1 is that the bamboo shoot pocket that goes mouldy that the embodiment of the invention provides removes the realization flow figure of mould method.
The specific embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further specified below in conjunction with accompanying drawing and embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in the qualification invention.
The bamboo shoot pocket that goes mouldy that Fig. 1 shows the embodiment of the invention to be provided removes the realization flow of mould method.
This method may further comprise the steps:
In step S101,54g is cut up with a hay cutter broken mouldy bamboo shoot pocket mix with the 6g wheat bran, the glucose that adds 0.6g is as carbon source; 0.9g urea as nitrogenous source; Under the condition of nature pH, inoculation yeast bacterium 2%-8%, bacillus subtilis 2%-8%; 35 ℃ of fermentation 24h-48h, this stage is called the aerobic fermentation stage;
In step S102, add glucose, the urea of 0.9g and the plant lactobacillus liquid of 2%-8% of 0.6g, with carbon dioxide emptying air, 35 ℃ of fermentation 24h-48h, this stage is called the anaerobic fermentation stage.
In embodiments of the present invention, mould bacterial content is 26 * 10 in the broken mouldy bamboo shoot pocket of 54g hand hay cutter 2Cfu/g, AFB 1Content is 3.20ug/g.
Below in conjunction with accompanying drawing and specific embodiment application principle of the present invention is further described.
A kind of bamboo shoot pocket that goes mouldy that Fig. 1 shows case study on implementation of the present invention to be provided removes the realization flow figure of mould method, and this method may further comprise the steps:
In step S101, at first carry out the aerobic fermentation stage
54g is cut up with a hay cutter broken mouldy bamboo shoot pocket (wherein mould bacterial content 26 * 10 2Cfu/g, AFB 1Content 3.20ug/g) and the 6g wheat bran put into container, the glucose that adds 0.6g is as carbon source, the urea of 0.9g is as nitrogenous source, natural pH, inoculation yeast bacterium 2%-8%, bacillus subtilis 2%-8%; 35 ℃ of ferment 24h or 48h.
In step S102, get into the anaerobic fermentation stage
The glucose that adds 0.6g, the plant lactobacillus liquid of the urea of 0.9g and 2%-8%, with carbon dioxide emptying air, 35 ℃ of fermentation 24h-48h.
The practical implementation step is: at first carry out the aerobic fermentation stage: 54g is cut up with a hay cutter broken mouldy bamboo shoot pocket (mould bacterial content 26 * 10 2Cfu/g, AFB 13.20ug/g) and the 6g wheat bran put into container, the glucose that adds 0.6g is as carbon source, the urea of 0.9g is as nitrogenous source, natural pH, inoculation yeast bacterium 2%-8%, bacillus subtilis 2%-8%; 35 ℃ of ferment 24h or 48h; Get into the anaerobic fermentation stage then: add the glucose of 0.6g again, the plant lactobacillus liquid of the urea of 0.9g and 2%-8%, with carbon dioxide emptying air, 35 ℃ of fermentation 24h-48h.
The bamboo shoot pocket that goes mouldy that the embodiment of the invention provides goes mould method; Adopt the asynchronous raw material fermentation of a kind of Mixed Microbes to go the technology of mould and mildew-resistant; At first carry out the aerobic fermentation stage: 54g is cut up with a hay cutter broken mouldy bamboo shoot pocket and 6g wheat bran put into container, the glucose that adds 0.6g is as carbon source, and the urea of 0.9g is as nitrogenous source; Inoculation yeast bacterium 2%-8% under the nature pH condition, bacillus subtilis 2%-8%; 35 ℃ of ferment 24h or 48h; Get into the anaerobic fermentation stage then, add the glucose of 0.6g, the plant lactobacillus liquid of the urea of 0.9g and 2%-8%, with carbon dioxide emptying air, 35 ℃ of fermentation 24h-48h; This method utilizes microbial fermentation bamboo shoot pocket for suppressing bamboo shoot pocket mould bacterial content, particularly aflatoxin degradation B 1Positive effect is arranged; Adopt the mode of microbial fermentation not only to have the mildew-resistant ability identical with mould inhibitor, and the toxin that exists originally in the bamboo shoot pocket of can degrading, more have superiority than adding the mould inhibitor mildew-resistant; Come mould fungus inhibition growth and degraded mycotoxin through microbial fermentation; Can avoid the nutrition destruction that the processing method of high temperature that present stage extensively adopts, strong acid, highly basic, adsorbent causes and the situation of secondary pollution; Reach safety and remove the purpose of mycotoxin, in this process, need not to bring into poisonous and hazardous chemical substance; Also reduce the loss of nutriment in the bamboo shoot pocket simultaneously as much as possible, have stronger popularization and using value.
The above is merely preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.

Claims (2)

1. the bamboo shoot pocket that goes mouldy goes mould method, it is characterized in that this method may further comprise the steps:
54g is cut up with a hay cutter broken mouldy bamboo shoot pocket mix with the 6g wheat bran, the glucose that adds 0.6g is as carbon source, and the urea of 0.9g is as nitrogenous source; Under the condition of nature pH, inoculation yeast bacterium 2%-8%, bacillus subtilis 2%-8%; 35 ℃ of fermentation 24h-48h, this stage is called the aerobic fermentation stage;
The glucose, the urea of 0.9g and the plant lactobacillus liquid of 2%-8% that add 0.6g, with carbon dioxide emptying air, 35 ℃ of fermentation 24h-48h, this stage is called the anaerobic fermentation stage.
2. the method for claim 1 is characterized in that, mould bacterial content is 26 * 10 in the broken mouldy bamboo shoot pocket of 54g hand hay cutter 2Cfu/g, AFB 1Content is 3.20ug/g.
CN2012103140423A 2012-08-24 2012-08-24 Method of removing mildew of molded bamboo shoot heart Pending CN102793122A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103333828A (en) * 2013-06-27 2013-10-02 中国农业大学 Application of anti-mildew bacillus strain and antimicrobial product thereof in corn storage
CN107183484A (en) * 2017-06-01 2017-09-22 烟台东和饲料科技有限公司 It is a kind of for the composition of feedstuff mildew, preparation method and its application method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1091913A (en) * 1993-03-11 1994-09-14 曾庆桂 Bamboo shoot food
CN1726808A (en) * 2004-07-30 2006-02-01 雷霖生 Method for prepearing delicious bamboo shoot
KR20110043040A (en) * 2009-10-20 2011-04-27 이오형 Isolation of microbes with wide range of antibiotic and digestive activities and method of manufacturing functional bamboo-fermented water using these microbes
CN102125167A (en) * 2010-12-30 2011-07-20 浙江大学 Method for preparing bamboo shell feed by using waste bamboo shells
CN102273573A (en) * 2011-08-15 2011-12-14 富阳市杭富罐头食品有限公司 Processing method of mixed silage

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1091913A (en) * 1993-03-11 1994-09-14 曾庆桂 Bamboo shoot food
CN1726808A (en) * 2004-07-30 2006-02-01 雷霖生 Method for prepearing delicious bamboo shoot
KR20110043040A (en) * 2009-10-20 2011-04-27 이오형 Isolation of microbes with wide range of antibiotic and digestive activities and method of manufacturing functional bamboo-fermented water using these microbes
CN102125167A (en) * 2010-12-30 2011-07-20 浙江大学 Method for preparing bamboo shell feed by using waste bamboo shells
CN102273573A (en) * 2011-08-15 2011-12-14 富阳市杭富罐头食品有限公司 Processing method of mixed silage

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
左瑞雨,等: "乳酸菌和枯草芽孢杆菌对黄曲霉毒素产生菌生长的抑制作用研究", 《河南农业科学》, vol. 40, no. 3, 31 December 2011 (2011-12-31), pages 145 - 148 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103333828A (en) * 2013-06-27 2013-10-02 中国农业大学 Application of anti-mildew bacillus strain and antimicrobial product thereof in corn storage
CN103333828B (en) * 2013-06-27 2014-08-27 中国农业大学 Application of anti-mildew bacillus strain and antimicrobial product thereof in corn storage
CN107183484A (en) * 2017-06-01 2017-09-22 烟台东和饲料科技有限公司 It is a kind of for the composition of feedstuff mildew, preparation method and its application method

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Application publication date: 20121128