CN116422682A - Microbial composite bacterial agent for rapid degradation of kitchen waste and use method thereof - Google Patents
Microbial composite bacterial agent for rapid degradation of kitchen waste and use method thereof Download PDFInfo
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- CN116422682A CN116422682A CN202211561199.6A CN202211561199A CN116422682A CN 116422682 A CN116422682 A CN 116422682A CN 202211561199 A CN202211561199 A CN 202211561199A CN 116422682 A CN116422682 A CN 116422682A
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- 239000002131 composite material Substances 0.000 title claims abstract description 67
- 239000010806 kitchen waste Substances 0.000 title claims abstract description 64
- 230000000813 microbial effect Effects 0.000 title claims abstract description 31
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 28
- 230000015556 catabolic process Effects 0.000 title claims abstract description 19
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 32
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 30
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 15
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 14
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 11
- 239000000654 additive Substances 0.000 claims abstract description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 239000001888 Peptone Substances 0.000 claims abstract description 4
- 108010080698 Peptones Proteins 0.000 claims abstract description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 4
- 235000015278 beef Nutrition 0.000 claims abstract description 4
- 230000001332 colony forming effect Effects 0.000 claims abstract description 4
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 4
- 239000004310 lactic acid Substances 0.000 claims abstract description 4
- 235000019319 peptone Nutrition 0.000 claims abstract description 4
- 239000010902 straw Substances 0.000 claims abstract description 4
- 239000000969 carrier Substances 0.000 claims abstract description 3
- 230000000996 additive effect Effects 0.000 claims abstract 2
- 238000003756 stirring Methods 0.000 claims description 22
- 238000004321 preservation Methods 0.000 claims description 15
- 230000000593 degrading effect Effects 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- 239000000440 bentonite Substances 0.000 claims description 11
- 229910000278 bentonite Inorganic materials 0.000 claims description 11
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 11
- 239000001913 cellulose Substances 0.000 claims description 9
- 229920002678 cellulose Polymers 0.000 claims description 9
- 239000010813 municipal solid waste Substances 0.000 claims description 8
- 239000003513 alkali Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 238000009629 microbiological culture Methods 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 239000004215 Carbon black (E152) Substances 0.000 abstract description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 3
- 238000009825 accumulation Methods 0.000 abstract description 3
- 229930195733 hydrocarbon Natural products 0.000 abstract description 3
- 150000002430 hydrocarbons Chemical class 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 230000009089 cytolysis Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000001877 deodorizing effect Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000004580 weight loss Effects 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 235000021384 green leafy vegetables Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- -1 nitrous acid amine Chemical class 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/70—Chemical treatment, e.g. pH adjustment or oxidation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/60—Biochemical treatment, e.g. by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B2101/00—Type of solid waste
- B09B2101/70—Kitchen refuse; Food waste
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
- C12R2001/385—Pseudomonas aeruginosa
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- General Chemical & Material Sciences (AREA)
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- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Processing Of Solid Wastes (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a microbial composite bacterial agent for quick degradation of kitchen waste and a use method thereof, comprising composite bacterial bodies, additives and carriers, wherein the composite bacterial bodies comprise photosynthetic bacteria, bacillus subtilis, pseudomonas aeruginosa and lysis agentBacillus amyloliquefaciens, cellulose-degrading bacteria and lactic acid bacteria, and colony forming units of 1×10 9 cfu/g, wherein the composite bacterial body accounts for 8-15% of the total amount of the composite bacterial agent; wherein the carrier is straw stalk powder, which accounts for 75-79% of the total amount of the composite microbial inoculum; the additive is peptone, beef extract, yeast powder and buffer, and accounts for 6-17% of the total amount of the composite microbial inoculum, and the pseudomonas aeruginosa can effectively degrade proteins and hydrocarbon substances in kitchen waste, wherein bacillus amyloliquefaciens can reduce nitrite accumulation, and the bacillus amyloliquefaciens and the pseudomonas aeruginosa play a role in promoting growth mutually, so that peculiar smell is reduced while kitchen waste is degraded.
Description
Technical Field
The invention relates to the technical field of kitchen waste degradation, in particular to a microbial composite bacterial agent for quick degradation of kitchen waste and a use method thereof.
Background
With the continuous growth of social economy and world population, the amount of kitchen waste is increased year by year, at present, more than 10 hundred million tons of kitchen waste is produced annually, and the main sources of kitchen waste are household daily life and various catering industries, wherein the kitchen waste is rich in organic matters such as starch, grease, protein and the like, and has higher recovery value.
Through retrieval, the invention patent with the Chinese patent number of CN114921356A discloses a high-efficiency kitchen waste degradation composite microbial agent, a preparation method and application thereof, wherein the composite microbial agent comprises a degradation microbial agent and a deodorizing microbial agent, and the degradation microbial agent comprises bacillus bailii spores, bacillus altitudis spores and bacillus cereus spores; the deodorant bacterial agent comprises bacillus subtilis spores, and the strains are independently separated and preserved in the invention.
Compared with the prior art, the Chinese patent No. CN114921356A has excellent degradation capability of protein, fat, starch and cellulose, and the bacillus subtilis also has high-efficiency NH removal function 3 And H 2 S capability. The composite microbial agent provided by the invention can be used for rapidly degrading kitchen waste, has no odor, and realizes harmless and reduction treatment of the kitchen waste.
However, in the actual use process of the device, the kitchen waste is decomposed and deodorized through the cooperation of the degrading bacterial agent and the deodorizing bacterial agent, but the kitchen waste is lack of treatment, so that the actual degradation efficiency of the bacterial agent is lower, and meanwhile, the selected bacterial cells are difficult to achieve the maximum degradation performance in the actual use, and the degradation effect on the kitchen waste is lower than a theoretical value, so that the microbial composite bacterial agent for rapidly degrading the kitchen waste and the use method thereof are provided.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a microbial composite bacterial agent for rapidly degrading kitchen waste and a use method thereof.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the microbial composite bacterial agent for rapid degradation of kitchen waste comprises composite bacterial bodies, additives and carriers, wherein the composite bacterial bodies comprise photosynthetic bacteria, bacillus subtilis, pseudomonas aeruginosa, bacillus amyloliquefaciens, cellulose degrading bacteria and lactic acid bacteria, colony forming units are 1 multiplied by 109cfu/g, and the composite bacterial bodies account for 8-15% of the total amount of the composite bacterial agent;
wherein the carrier is straw stalk powder, which accounts for 75-79% of the total amount of the composite microbial inoculum;
wherein the additives are peptone, beef extract, yeast powder and buffer, which account for 6-17% of the total amount of the composite microbial inoculum, and the additives are used for improving the overall activity and nutrition of the composite microbial inoculum.
The photosynthetic bacteria are preserved in China Center for Type Culture Collection (CCTCC) No: m2018604;
the pseudomonas aeruginosa is preserved in Shanghai preservation biotechnology center, and the strain number is SHBCC D15767;
the bacillus amyloliquefaciens is preserved in China center for type culture collection, wherein the preservation unit address is Wuhan in China, the preservation date is 5 months and 28 days in 2020, and the preservation number is CCTCCNO: M2020153;
the preservation number of the cellulose degrading bacteria is CCTCC NO: M20211094;
the lactobacillus is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.4430;
the bacillus subtilis is preserved in China general microbiological culture Collection center (CGMCC) No.25364, and the preservation date is 2022, 7, and 20.
The technical scheme further comprises the following steps:
the ratio of photosynthetic bacteria, bacillus subtilis, pseudomonas aeruginosa, bacillus amyloliquefaciens, cellulose degrading bacteria and lactobacillus is 1:1: (1.2-1.4):2:2: (1.7-1.9): 2.3.
the application method of the microbial composite microbial agent for rapid degradation of kitchen waste comprises the following steps:
the first step: alkali treatment of kitchen waste: placing the garbage in an alkaline environment for pretreatment;
and a second step of: adding bentonite and biochar into kitchen waste subjected to alkali treatment for stirring for 4-6h;
and a third step of: adding the prepared composite microbial inoculum into kitchen waste, and stirring to uniformly mix the kitchen waste and the composite microbial inoculum;
fourth step: and conveying the mixed materials to a fermentation area for stacking.
The treatment time in alkaline environment was 25℃for 24 hours with stirring.
While the alkaline environment is provided by CaO, wherein the content of CaO is as follows: 100g kitchen waste uses 2.8-3.1g CaO.
Wherein the stirring time of the kitchen waste and the composite microbial inoculum is 3-5h, the proportion of the composite microbial inoculum to the kitchen waste is (1.0-1.1): 200, and the use amount of the composite microbial inoculum is reduced due to alkali treatment and bentonite.
The piled materials are kept at 40-60 ℃ and are turned over and stirred once every 24 hours, and the total turning over and stirring is carried out for 3 times;
wherein, turn over and mix for many times in order to reduce the material and pile up inside high temperature for a long time.
The invention has the following beneficial effects:
1. according to the invention, the alkaline treatment of kitchen waste is used for promoting the high-solid anaerobic digestion, so that substrate cells can be destroyed, the hydrolysis of complex macromolecular organic matters is accelerated, the generation of volatile fatty acid is promoted, and bentonite and biochar are added later, and due to the large surface area and the porosity, the bentonite can provide sufficient habitat for the growth of microorganisms, so that the reaction efficiency between the subsequent microorganism composite microbial inoculum and the kitchen waste is increased.
2. In the invention, when the microbial composite microbial agent is used, photosynthetic bacteria rich in microbial composite microbial agent can convert nitrous acid amine into nontoxic compounds, wherein pseudomonas aeruginosa can effectively degrade proteins and hydrocarbon substances in kitchen waste, bacillus amyloliquefaciens can reduce nitrite accumulation, and bacillus amyloliquefaciens and pseudomonas aeruginosa play a role in promoting growth, so that peculiar smell is reduced while kitchen waste is degraded.
Detailed Description
The present embodiments will now be described more fully hereinafter with reference to the accompanying drawings, in which it is shown, however, that the embodiments shown are merely examples of the invention, and not all of the examples. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Firstly, placing garbage in an alkaline environment for pretreatment, wherein the treatment time in the alkaline environment is 25 ℃ for 24 hours, and stirring is carried out;
while the alkaline environment is provided by CaO, wherein the content of CaO is as follows: 100g kitchen garbage uses 2.8-3.1g CaO
And secondly, adding bentonite and biochar into the kitchen waste subjected to alkali treatment for stirring for 4-6 hours, wherein the proportion of the bentonite to the biochar is 3:1, and the total amount of the bentonite and the biochar is 3.3-4.7 percent of the kitchen waste.
The content of SCOD in the kitchen garbage after the treatment is 735678.1mg/L and the content of N is 873.2mg/L;
the alkaline pretreatment promotes the conversion of the nondegradable protein to soluble protein, thereby releasing ammonium particles during hydrolysis.
Example two
Placing the garbage in an alkaline environment for pretreatment, wherein the treatment time in the alkaline environment is 25 ℃ and 24 hours, and stirring is carried out;
while the alkaline environment is provided by CaO, wherein the content of CaO is as follows: 100g kitchen garbage uses 2.8-3.1g CaO
The content of SCOD in the kitchen garbage treated by the method is 61337.7mg/L, and the content of N is 617.4mg/L.
Comparative example one
Piling up kitchen wastes, and primarily stirring for 5-6h.
Example III
Adding the prepared composite microbial inoculum into the kitchen waste treated in the first embodiment, and stirring to uniformly mix the kitchen waste and the composite microbial inoculum;
wherein the stirring time of the kitchen waste and the composite microbial inoculum is 3-5h, the proportion of the composite microbial inoculum to the kitchen waste is (1.0-1.1) 200,
and conveying the mixed materials to a fermentation area for stacking, keeping the temperature of the stacked materials at 40-60 ℃, and stirring once every 24 hours for 3 times.
Wherein the composite bacterial body comprises photosynthetic bacteria, bacillus subtilis, pseudomonas aeruginosa, bacillus amyloliquefaciens, cellulose degrading bacteria and lactobacillus, and the colony forming unit is 1 multiplied by 10 9 cfu/g, wherein the composite bacterial body accounts for 8-15% of the total amount of the composite bacterial agent;
wherein the carrier is straw stalk powder, which accounts for 75-79% of the total amount of the composite microbial inoculum;
wherein the additives are peptone, beef extract, yeast powder and buffer agent, which account for 6-17% of the total amount of the composite microbial inoculum;
the ratio of photosynthetic bacteria, bacillus subtilis, pseudomonas aeruginosa, bacillus amyloliquefaciens, cellulose degrading bacteria and lactobacillus is 1:1: (1.2-1.4):2:2: (1.7-1.9): 2.3.
example IV
Adding the prepared composite microbial inoculum into the kitchen waste treated in the second embodiment, and stirring to uniformly mix the kitchen waste and the composite microbial inoculum;
wherein the stirring time of the kitchen waste and the composite microbial inoculum is 3-5h, the proportion of the composite microbial inoculum to the kitchen waste is (1.0-1.1) 200,
conveying the mixed materials to a fermentation area for stacking, keeping the temperature of the stacked materials at 40-60 ℃, and stirring once every 24 hours for 3 times
Comparative example two
Adding the prepared composite microbial inoculum into the kitchen waste treated in the first comparative example, and stirring to uniformly mix the kitchen waste and the composite microbial inoculum;
wherein the stirring time of the kitchen waste and the composite microbial inoculum is 3-5h, the proportion of the composite microbial inoculum to the kitchen waste is (1.0-1.1) 200,
and conveying the mixed materials to a fermentation area for stacking, keeping the temperature of the stacked materials at 40-60 ℃, and stirring once every 24 hours for 3 times.
| Project | Example III | Example IV | Comparative example two |
| Starch weight loss percentage% | 80.49 | 78.42 | 74.31 |
| Green leaf vegetables weight loss rate% | 80.31 | 76.23 | 74.11 |
| Total subtraction ofWeight ratio of | 81.36 | 76.29 | 69.31 |
| Odor rating | Slight | Slight | Slight |
The alkaline treatment of kitchen waste promotes high-solid anaerobic digestion, so that substrate cells can be destroyed, hydrolysis of complex macromolecular organic matters is accelerated, volatile fatty acid is promoted, and bentonite and biochar added later can provide sufficient habitat for microorganism growth due to large surface area and porosity, so that the reaction efficiency between the subsequent microorganism composite microbial inoculum and kitchen waste is improved;
the photosynthetic bacteria rich in the microbial composite bacterial agent can convert nitrous amine into a nontoxic compound, wherein pseudomonas aeruginosa can effectively degrade proteins and hydrocarbon substances in kitchen waste, bacillus amyloliquefaciens can reduce nitrite accumulation, and bacillus amyloliquefaciens and pseudomonas aeruginosa play a role in promoting growth mutually, so that peculiar smell is reduced while kitchen waste is degraded.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The microbial composite microbial agent for rapidly degrading kitchen waste is characterized by comprising composite bacterial bodies, additives and carriers, wherein the composite bacterial bodies comprise photosynthetic fine particlesBacteria, bacillus subtilis, pseudomonas aeruginosa, bacillus amyloliquefaciens, cellulose degrading bacteria and lactic acid bacteria, and colony forming unit is 1 multiplied by 10 9 cfu/g, wherein the composite bacterial body accounts for 8-15% of the total amount of the composite bacterial agent;
wherein the carrier is straw stalk powder, which accounts for 75-79% of the total amount of the composite microbial inoculum;
wherein the additive is peptone, beef extract, yeast powder and buffer agent, which account for 6-17% of the total amount of the composite microbial inoculum.
The photosynthetic bacteria are preserved in China Center for Type Culture Collection (CCTCC) No: m2018604;
the bacillus subtilis is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.25364 and the preservation date of 2022, 7 and 20;
the pseudomonas aeruginosa is preserved in Shanghai preservation biotechnology center, and the strain number is SHBCC D15767;
the bacillus amyloliquefaciens is preserved in China center for type culture collection, wherein the preservation unit address is Wuhan in China, the preservation date is 5 months and 28 days in 2020, and the preservation number is CCTCCNO: M2020153;
the preservation number of the cellulose degrading bacteria is CCTCC NO: M20211094;
the lactobacillus is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.4430.
2. The microbial composite inoculant for rapid degradation of kitchen waste and the use method thereof according to claim 1, wherein the ratio of photosynthetic bacteria, bacillus subtilis, pseudomonas aeruginosa, bacillus amyloliquefaciens, cellulose degrading bacteria and lactic acid bacteria is 1:1: (1.2-1.4):2:2: (1.7-1.9): 2.3.
3. the application method of the microbial composite microbial agent for rapid degradation of kitchen waste is characterized by comprising the following steps of:
the first step: alkali treatment of kitchen waste: placing the garbage in an alkaline environment for pretreatment;
and a second step of: adding bentonite and biochar into the kitchen waste subjected to alkali treatment for stirring for 4-6 hours, wherein the proportion of the bentonite to the biochar is 3:1, and the total amount of the bentonite and the biochar is 3.3-4.7%;
and a third step of: adding the prepared composite microbial inoculum into kitchen waste, and stirring to uniformly mix the kitchen waste and the composite microbial inoculum;
fourth step: and conveying the mixed materials to a fermentation area for stacking.
4. The method of claim 3, wherein the alkaline environment is treated at 25 ℃ for 24 hours with stirring.
5. The method for using the microbial composite inoculant for rapid degradation of kitchen waste according to claim 3, wherein the alkaline environment is provided by CaO, and the content of CaO is as follows: 100g kitchen waste uses 2.8-3.1g CaO.
6. The method for using the microbial composite microbial agent for rapid degradation of kitchen waste according to claim 3, wherein the stirring time of the kitchen waste and the composite microbial agent is 3-5h, and the ratio of the composite microbial agent to the kitchen waste is (1.0-1.1): 200.
7. The method for using the microbial composite agent for rapid degradation of kitchen waste according to claim 3, wherein the temperature of the piled materials is kept at 40-60 ℃, and the materials are stirred once every 24 hours for 3 times.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211561199.6A CN116422682A (en) | 2022-12-07 | 2022-12-07 | Microbial composite bacterial agent for rapid degradation of kitchen waste and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115537354A (en) * | 2022-09-30 | 2022-12-30 | 贵州大学 | Biocontrol bacterium microcapsule microbial inoculum, preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115537354A (en) * | 2022-09-30 | 2022-12-30 | 贵州大学 | Biocontrol bacterium microcapsule microbial inoculum, preparation method and application |
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