CN102772554B - Traditional Chinese medicine compound for curing schizophrenia - Google Patents
Traditional Chinese medicine compound for curing schizophrenia Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine compound for curing schizophrenia, comprising the following bulk pharmaceutical chemicals in parts by weight: 1-90 parts of gentian, 1-50 parts of radix bupleuri, 1-90 parts of radix rehmanniae, 1-240 parts of magnet and 1-60 parts of Salvia miltiorrhiza. The compounds can be added or cut for adjusting according to practical conditions, and can be selected from any traditional Chinese medicine compound containing gentian, radix bupleuri, radix rehmanniae, magnet and Salvia miltiorrhiza. The compound can be adjusted through adding or removing the components according to practical conditions, and can be selected from any traditional Chinese medicinal compound comprising gentian, radix bupleuri, radix rehmanniae, magnet and Salvia miltiorrhiza or compound further with other added components or with the above removed components, preferably decoction of all the given bulk pharmaceutical chemicals, wherein the decoction can be obtained through a decocting method. Components adding and removing of the traditional Chinese medicine compound disclosed by the invention is similar to risperidone in effect. The traditional Chinese medicine compound has effects on the autonomic activities, space learning and memory improvement of a model rat. The mechanism may be related to the adjustment of DA, DOPA, HVA, 5-HT and 5-HIAA contents in the corpus striatum of the model rat.
Description
Technical field
The invention belongs to schizoid treatment technology field, relate to one and be used for the treatment of schizoid Chinese medicine composition.
Background technology
Schizophrenia, is once called as dementia schizophrenia, is the not bright psychiatric condition of a kind of cause of disease.A lot of disease, in person between twenty and fifty, often has the inharmonious of many-sided obstacles such as perception, thinking, emotion, behavior and ergasia.Generally do not realize and disturbance of intelligence, the many delays of the course of disease.This disease is the one that in each mattoid, the state of an illness is the heaviest, and in population of China, prevalence is about 1%, and annual morbidity is about 0.1 ‰.The schizoid prevalence in another town and country is all negative correlation with household economy level, and the average prevalence of resident that namely economic level is low is high, and the average prevalence of the resident that economic level is high is low.Meanwhile, the schizophrenia course of disease is long, recurrent exerbation, and hallucination is its modal symptom.
Schizoid treatment comprises Drug therapy and psychotherapy, and its Chinese medicine can recover ability of thinking, emotional expression and insight effectively, and has eliminating effect to hallucination.For recurrent exerbation schizophrenic, psychotherapy is adopted to be difficult to without insight schizophrenic effective.So in schizoid treatment and control procedure, Drug therapy occupy leading decisive status always.
Although the medicine being used for the treatment of schizophrenia disease at present has many types, how many effective Western medicine can be attended by the untoward reaction such as extrapyramidal system side reaction, body weight increase.While treatment is controlled to the symptoms of schizophrenia, investigation and application untoward reaction being reduced to the Chinese medicinal formulae of incidence rate have also been obtained promotion, particularly the application of original clinical conventional experience ancient prescription plus-minus taste, improves the drawback of Western medicine in treatment of schizophrenia to a great extent.
Summary of the invention
The problem that the present invention solves is to provide one to be used for the treatment of schizoid Chinese medicine composition, select there is treatment emotional lability, natural edible-plant medicine that auditory hallucination is abundant is used as medicine, and carry out screening through clinical verification repeatedly and form, can be used for treatment schizophrenia and eliminate hallucination.
The present invention is achieved through the following technical solutions:
One is used for the treatment of schizoid Chinese medicine composition, in mass fraction, comprises the crude drug of following component:
Radix Gentianae 1 ~ 90 part, Radix Bupleuri 1 ~ 50 part, 1 ~ 90 part, the Radix Rehmanniae, Magnetitum 1 ~ 240 part and Radix Salviae Miltiorrhizae 1 ~ 60 part.
Further, above-mentioned prescription carries out interpolation taste again.
Also add the crude drug of following component:
Semen Ziziphi Spinosae (parched) 1 ~ 90 part, the Radix Angelicae Dahuricae 1 ~ 90 part, 1 ~ 50 part, Poria, Ramulus Uncariae Cum Uncis 1 ~ 60 part, Rhizoma Dioscoreae 1 ~ 90 part, Semen Myristicae 1 ~ 90 part, Fructus Ligustri Lucidi 1 ~ 50 part, 1 ~ 30 part, Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae 1 ~ 30 part, Rhizoma Acori Graminei 1 ~ 30 part, Cortex Albiziae 1 ~ 30 part, Rhizoma Chuanxiong 1 ~ 30 part of magnolia obovata 1 ~ 30 part.
Or add the crude drug of following component:
Pericarpium Citri Reticulatae 1 ~ 30 part, Semen Coicis 1 ~ 20 part and Rhizoma Alismatis 1 ~ 50 part.And add further: Rhizoma Dioscoreae 1 ~ 90 part, Semen Myristicae 1 ~ 90 part, Fructus Ligustri Lucidi 1 ~ 50 part, the Rhizoma Anemarrhenae 1 ~ 30 part and Cortex Cinnamomi 1 ~ 10 part.
Further, the crude drug of one of following two kinds of components is added:
First component: 1 ~ 30 part, Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae 1 ~ 30 part, Rhizoma Acori Graminei 1 ~ 30 part, Cortex Albiziae 1 ~ 30 part, Rhizoma Chuanxiong 1 ~ 30 part of magnolia obovata 1 ~ 30 part.
Second component: Ramulus Uncariae Cum Uncis 1 ~ 60 part, Arillus Longan 1 ~ 30 part, Cortex Moutan 1 ~ 30 part, Fructus Schisandrae Chinensis 1 ~ 30 part, Radix Achyranthis Bidentatae 1 ~ 30 part and Poria cum Radix Pini 1 ~ 30 part.
One is used for the treatment of schizoid Chinese medicine composition, in mass fraction, comprises the crude drug of following component:
Radix Gentianae 2 ~ 80 parts, Radix Bupleuri 5 ~ 40 parts, 2 ~ 90 parts, the Radix Rehmanniae, Magnetitum 10 ~ 200 parts, Radix Salviae Miltiorrhizae 10 ~ 50 parts, Semen Ziziphi Spinosae (parched) 2 ~ 80 parts, the Radix Angelicae Dahuricae 2 ~ 85 parts, 3 ~ 40 parts, Poria, Ramulus Uncariae Cum Uncis 2 ~ 40 parts, Rhizoma Dioscoreae 2 ~ 80 parts, Semen Myristicae 2 ~ 80 parts and Fructus Ligustri Lucidi 3 ~ 40 parts.
One is used for the treatment of schizoid Chinese medicine composition, in mass fraction, comprises the crude drug of following component:
Radix Gentianae 2 ~ 80 parts, Radix Bupleuri 5 ~ 40 parts, 2 ~ 90 parts, the Radix Rehmanniae, Magnetitum 10 ~ 200 parts, Radix Salviae Miltiorrhizae 10 ~ 50 parts, Semen Ziziphi Spinosae (parched) 2 ~ 80 parts, the Radix Angelicae Dahuricae 2 ~ 85 parts, 3 ~ 40 parts, Poria, Ramulus Uncariae Cum Uncis 2 ~ 40 parts, Rhizoma Dioscoreae 2 ~ 80 parts, Semen Myristicae 2 ~ 80 parts, Fructus Ligustri Lucidi 3 ~ 40 parts, the Rhizoma Anemarrhenae 2 ~ 30 parts, Cortex Cinnamomi 1 ~ 10 part, Pericarpium Citri Reticulatae 1 ~ 30 part, Semen Coicis 1 ~ 20 part and Rhizoma Alismatis 2 ~ 50 parts.
One is used for the treatment of schizoid Chinese medicine composition, in mass fraction, comprises the crude drug of following component:
Radix Gentianae 90 parts, Radix Bupleuri 45 parts, 90 parts, the Radix Rehmanniae, Magnetitum 240 parts, Radix Salviae Miltiorrhizae 30 parts, Semen Ziziphi Spinosae (parched) 90 parts, the Radix Angelicae Dahuricae 30 parts, 50 parts, Poria, Ramulus Uncariae Cum Uncis 30 parts, Rhizoma Dioscoreae 90 parts, Semen Myristicae 45 parts and Fructus Ligustri Lucidi 30 parts.
Described is used for the treatment of schizoid Chinese medicine composition, and after each taste crude drug is pressed proportion by weight, decocting with water to its volume concentration is 1/4 ~ 1/5, obtains the extract decocted, directly takes;
Or using the extract decocted as effective ingredient, add or do not add adjuvant and make oral agents, described oral agents comprises pill, capsule, tablet, powder, granule, decoction or oral liquid.
Described be used for the treatment of schizoid Chinese medicine composition preparation for or treatment schizophrenia and/or eliminate the application of medicine of hallucination.
Described is used for the treatment of schizoid Chinese medicine composition regulates the medicine of neurotransmitter application in preparation; Described neurotransmitter comprises one or more in DA, DOPA, HVA, 5-HT and 5-HIAA.
Compared with prior art, the present invention has following useful technique effect:
Provided by the inventionly be used for the treatment of schizoid Chinese medicine composition, based on Traditional Chinese Medicine to the understanding of schizophrenic onset mechanism and Therapeutic Principle, filter out there is treatment emotional lability, natural edible-plant medicine that auditory hallucination is abundant is used as medicine, and through clinical verification repeatedly, therapeutic effect is good.
For verifying Chinese medicine composition of the present invention plus-minus taste medicinal effects, except stereotyped behavior, the experiment of spacious field and Morris water maze laboratory are evaluated except the change of rat behavior, also this composition is carried out to the change of coulomb array electrochemical high-performance liquid chromatogram determination intrastriatal neural mediator content.Select the method for MK-801 chronic low-dose administration to make schizophrenia rats model, and select risperidone to be contrast medicine, on Chinese medicine composition plus-minus, taste affects rat model autonomic activities, Spatial learning and memory effect is verified.Experimental result shows, and on MK-801 schizophrenia rats model, Chinese medicine composition plus-minus taste of the present invention is similar to risperidone effect.Effect is had to rat model autonomic activities, Spatial learning and memory improvement.Its mechanism may be relevant with the adjustment that take part in rat model intrastriatal DA, DOPA, HVA, 5-HT and 5-HIAA content.
Detailed description of the invention
The invention provides and be used for the treatment of schizoid Chinese medicine composition, plus-minus taste can be carried out according to practical situation to adjust: can from any containing the Chinese medicine compositions such as Radix Gentianae, Radix Bupleuri, the Radix Rehmanniae, Magnetitum, Radix Salviae Miltiorrhizae and plus-minus taste in provided Chinese prescription, the decoction of preferably given crude drug Chinese crude drug, can be obtained by the method decocted.Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
1, Chinese medicine composition embodiment
Embodiment 1
In mass fraction, comprise following component:
Radix Gentianae 90g, Radix Bupleuri 45g, Radix Rehmanniae 90g, Magnetitum 240g, Radix Salviae Miltiorrhizae 30g;
After said components is mixed, decoct with water and make decoction.Add medicine to the 5000mL that adds water, and decocts to 1000mL.Each serving with 50ml, every day 1 ~ 2 time.
Embodiment 2
Radix Gentianae 90g, Radix Bupleuri 45g, Radix Rehmanniae 90g, Magnetitum 240g, Radix Salviae Miltiorrhizae 30g, Semen Ziziphi Spinosae (parched) 90g, Radix Angelicae Dahuricae 30g, Poria 50g, Ramulus Uncariae Cum Uncis 30 parts, Rhizoma Dioscoreae 90g, Semen Myristicae 45g, Fructus Ligustri Lucidi 30g.
After said components is mixed, decoct with water and make decoction.Add medicine to the 5000mL that adds water, and decocts to 1000mL.Each serving with 50ml, every day 1 ~ 2 time.
Embodiment 3
Radix Gentianae 90 parts, Radix Bupleuri 50 parts, 90 parts, the Radix Rehmanniae, Magnetitum 240 parts, Radix Salviae Miltiorrhizae 60 parts, Pericarpium Citri Reticulatae 30 parts, Semen Coicis 20 parts, Rhizoma Alismatis 50 parts.
Magnetitum first under in 5000mL water, treat that water boils, after the mixing of above-mentioned all the other components, add.Decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Embodiment 4
Radix Gentianae 80 parts, Radix Bupleuri 40 parts, 60 parts, the Radix Rehmanniae, Magnetitum 200 parts, Radix Salviae Miltiorrhizae 30 parts, Semen Ziziphi Spinosae (parched) 90 parts, Ramulus Uncariae Cum Uncis 30 parts, Rhizoma Dioscoreae 90 parts, Rhizoma Alismatis 50 parts.
After said components is mixed, decoct with water and make decoction.Add medicine to the 5000mL that adds water, and decocts to 1200mL.Each serving with 50ml, every day 1 ~ 2 time.
Embodiment 5
Radix Gentianae 90 parts, Radix Bupleuri 50 parts, 90 parts, the Radix Rehmanniae, Magnetitum 240 parts, Pericarpium Citri Reticulatae 30 parts, Semen Coicis 20 parts, Rhizoma Alismatis 50 parts, Rhizoma Dioscoreae 90 parts, Semen Myristicae 90 parts, Fructus Ligustri Lucidi 50 parts, the Rhizoma Anemarrhenae 30 parts, Cortex Cinnamomi 10 parts.
By said components mix after, the 2000mL that adds water decoct to 1/3, pour out medicinal liquid, continuous three times as method operate.Merge three medicinal liquids, decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Embodiment 6
Radix Gentianae 60 parts, Radix Bupleuri 30 parts, 50 parts, the Radix Rehmanniae, Magnetitum 180 parts, Radix Salviae Miltiorrhizae 20 parts, Semen Ziziphi Spinosae (parched) 20 parts, the Radix Angelicae Dahuricae 10 parts, 8 parts, Poria, Ramulus Uncariae Cum Uncis 15 parts, Rhizoma Dioscoreae 30 parts, Semen Myristicae 25 parts and Fructus Ligustri Lucidi 3 parts.
By said components mix after, the 2000mL that adds water decoct to 1/3, pour out medicinal liquid, continuous three times as method operate.Merge three medicinal liquids, decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Embodiment 7
Radix Gentianae 50 parts, Radix Bupleuri 25 parts, 60 parts, the Radix Rehmanniae, Magnetitum 150 parts, Radix Salviae Miltiorrhizae 25 parts, Semen Ziziphi Spinosae (parched) 10 parts, the Radix Angelicae Dahuricae 20 parts, 15 parts, Poria, Ramulus Uncariae Cum Uncis 10 parts, Rhizoma Dioscoreae 10 parts, Semen Myristicae 15 parts and Fructus Ligustri Lucidi 10 parts.
By said components mix after, the 2000mL that adds water decoct to 1/3, pour out medicinal liquid, continuous three times as method operate.Merge three medicinal liquids, decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Embodiment 8
Radix Gentianae 50 parts, Radix Bupleuri 25 parts, 60 parts, the Radix Rehmanniae, Magnetitum 150 parts, Radix Salviae Miltiorrhizae 25 parts, Semen Ziziphi Spinosae (parched) 10 parts, the Radix Angelicae Dahuricae 20 parts, 15 parts, Poria, Ramulus Uncariae Cum Uncis 10 parts, Rhizoma Dioscoreae 10 parts, Semen Myristicae 15 parts and Fructus Ligustri Lucidi 10 parts.
By said components mix after, the 2000mL that adds water decoct to 1/3, pour out medicinal liquid, continuous three times as method operate.Merge three medicinal liquids, decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Embodiment 9
Radix Gentianae 80 parts, Radix Bupleuri 50 parts, 60 parts, the Radix Rehmanniae, Magnetitum 200 parts and Radix Salviae Miltiorrhizae 50 parts, Semen Ziziphi Spinosae (parched) 30 parts, the Radix Angelicae Dahuricae 40 parts, 10 parts, Poria, Ramulus Uncariae Cum Uncis 20 parts, Rhizoma Dioscoreae 30 parts, Semen Myristicae 20 parts and Fructus Ligustri Lucidi 5 parts, 20 parts, Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae 10 parts, Rhizoma Acori Graminei 10 parts, Cortex Albiziae 3 parts, Rhizoma Chuanxiong 10 parts of magnolia obovatas 5 parts.
Magnetitum first under in 5000mL water, treat that water boils, add after the mixing of above-mentioned all the other components.Decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Embodiment 10
Radix Gentianae 80 parts, Radix Bupleuri 50 parts, 60 parts, the Radix Rehmanniae, Magnetitum 200 parts, Radix Salviae Miltiorrhizae 50 parts, Pericarpium Citri Reticulatae 10 parts, Semen Coicis 10 parts and Rhizoma Alismatis 20 parts, Rhizoma Dioscoreae 10 parts, Semen Myristicae 5 parts, Fructus Ligustri Lucidi 15 parts, the Rhizoma Anemarrhenae 20 parts and Cortex Cinnamomi 2 parts, Ramulus Uncariae Cum Uncis 10 parts, Arillus Longan 2 parts, Cortex Moutan 10 parts, Fructus Schisandrae Chinensis 10 parts, Radix Achyranthis Bidentatae 10 parts and Poria cum Radix Pini 10 parts.
By said components mix after, the 2000mL that adds water decoct to 1/3, pour out medicinal liquid, continuous three times as method operate.Merge three medicinal liquids, decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Embodiment 11
Radix Gentianae 50 parts, Radix Bupleuri 30 parts, 40 parts, the Radix Rehmanniae, Magnetitum 200 parts, Radix Salviae Miltiorrhizae 50 parts, Pericarpium Citri Reticulatae 12 parts, Semen Coicis 15 parts and Rhizoma Alismatis 25 parts, Rhizoma Dioscoreae 20 parts, Semen Myristicae 15 parts, Fructus Ligustri Lucidi 10 parts, the Rhizoma Anemarrhenae 10 parts and Cortex Cinnamomi 10 parts, Ramulus Uncariae Cum Uncis 12 parts, Arillus Longan 12 parts, Cortex Moutan 15 parts, Fructus Schisandrae Chinensis 8 parts, Radix Achyranthis Bidentatae 5 parts and Poria cum Radix Pini 5 parts.
By said components mix after, the 2000mL that adds water decoct to 1/3, pour out medicinal liquid, continuous three times as method operate.Merge three medicinal liquids, decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Embodiment 12
Radix Gentianae 50 parts, Radix Bupleuri 30 parts, 40 parts, the Radix Rehmanniae, Magnetitum 200 parts, Radix Salviae Miltiorrhizae 50 parts, Semen Ziziphi Spinosae (parched) 30 parts, the Radix Angelicae Dahuricae 40 parts, 10 parts, Poria, Ramulus Uncariae Cum Uncis 20 parts, Rhizoma Dioscoreae 30 parts, Semen Myristicae 20 parts and Fructus Ligustri Lucidi 5 parts, 20 parts, Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae 10 parts, Rhizoma Acori Graminei 10 parts, Cortex Albiziae 3 parts, Rhizoma Chuanxiong 10 parts of magnolia obovatas 5 parts.
Magnetitum first under in 5000mL water, treat that water boils, add after the mixing of above-mentioned all the other components.Decoct to 1000mL.Each 50ml, every day 1 ~ 2 time.
Above-mentioned being decocts and obtains, and also using the extract decocted as effective ingredient, can add or do not add adjuvant and make pill, capsule, tablet, powder, granule, decoction or oral liquid.
And the scheme that in above-mentioned Chinese medicine plus-minus taste, preferably Chinese medicine kind is more, i.e. embodiment 2-12, is wherein more preferably embodiment 9-12.
Concrete, the experimental verification carrying out below with the extract prepared by embodiment 2.
2, on the ethological impact of schizophrenia rat model
The preparation of 2.1 animal model of schizophrenia
SD rat, female, 52.Environmental adaptation 1 week, weighs, and is divided into 4 groups by body weight layering, often organizes 13.Select MK-801 ([(+)-5-methyl-10,11-dibenzol a, d-cyclohepten-5,10-imine meleate], maleate; Chinese name dizocilpine) as instrument medicine, according to 0.05mg/mL configuration, be dissolved in normal saline.Every day injects 0.1mg/kg, in wherein three groups of rats, within every three days, weighs once, according to body weight adjustment dose, continuously injection 14 days.Carry out random following grouping afterwards: model control group, Chinese medicine composition plus-minus taste group, risperidone group; And with do not inject MK-801 for blank group.
Lumbar injection normal saline is given, continuously injection 14 days according to body weight.Be injected in every day and early complete 9 point ~ morning 10.Injection terminates time sky and carries out behavioristics's evaluation.
2.2 Drug therapys and behavioristics's evaluation.
Every day early completes each group of rat oral gavage 9 point ~ morning 10.The wherein normal saline of blank group and model control group gavage 4mL/kg; The Chinese medicine composition plus-minus taste soup of Chinese medicine composition plus-minus taste group gavage 3g crude drug/mL, 4mL/kg; Risperidone group gives the Risperidone Oral Solution of 0.2mg/4mL/kg, continuous 4 weeks.
The experiment of spacious field:
Each for length and width 100cm, high 50cm, bottom surface are divided into the wooden case of 25 equilateral grids of 10cm × 10cm, from the activity of motion tracking rat, and Auto-counting.Need to observe in quiet room, rat is put into center grid, observe the quantity that rat passes through grid in 10min, represent rat autonomic activities, if increase, be considered as being similar to the schizoid positive symptom.
Morris water maze:
Morris water maze is that Britain psychologist Morris designs and is applied to the research of learning and memory brain mechanism.Experiment can be more for the parameter analyzed, comprise escape latency, all quadrants swimming distance, original platform quadrant swim distance and total ratio of distances constant, the ratio of original platform quadrant swim time and total time, platform deflecting angle, cross-platform number of times and in, outer shroud swim apart from percentage ratio etc.Platform required time is found to assess the learning capacity of animal in water maze according to rat in training; Time according to the cruise cost of rat in platform quadrant can assess the extraction situation of rat to spatial memory.
2.3 models are set up and drug effect evaluation
Rat through lumbar injection MK-801 shows autonomic activities than the rat of injecting normal saline group and increases (P<0.05) in the experiment of spacious field, illustrates that MK-801 induces rat kind and is similar to the schizoid positive symptom (see table 1-1).
In Morris water maze laboratory, the learning capacity of rat and spatial memory extract and all change, but change without significance.Illustrate that MK-801 can cause certain memory impaired (see table 1-2) to rat.
After Chinese medicine composition plus-minus taste soup and risperidone treatment, the autonomic activities of rat declines, with matched group no difference of science of statistics, Chinese medicine composition plus-minus taste soup group no difference of science of statistics compared with risperidone group, illustrate that Chinese medicine composition can improve the positive symptom of schizophrenia rat model, with risperidone group no difference of science of statistics (see showing 1-1).
And in Morris water maze laboratory, there is learning capacity decline in model group rats, memory retrieval ability declines, show the prolongation of latency finding escape platform in the training process respectively, spend in the time shorten of target quadrant, after Chinese medicine composition plus-minus taste soup and risperidone treatment, symptom is obviously improved, illustrate that Chinese medicine composition plus-minus taste soup improves the memory impairment of rat model, and with risperidone no difference of science of statistics (see showing 1-2).
Table 1-1 open field test result
| Group | Number of elements | Model evaluation is across lattice sum | Drug effect evaluation is across lattice sum |
| Matched group | 13 | 253.2±121.3 | 226.3±98.3 |
| Model group | 13 | 326.8±74.2 * | 315.1±65.9 * |
| Chinese medicine composition plus-minus taste group | 12 | 337.1±78.7 * | 243.8±86.8 |
| Risperidone group | 10 | 334.2±49.2 * | 242.1±89.6 |
Note:
*p<0.05 compared with matched group
Table 1-2Morris water maze test result (unit: ms)
Note:
*p<0.05 compared with matched group
In the experiment of spacious field and Morris water maze laboratory, prove that Chinese medicine composition plus-minus taste soup can increase the autonomic activities of MK-801 rat model, improve the memory impairment of MK-801 rat model.
3, Chinese medicine composition plus-minus taste is on the impact of mediator content in schizophrenia rat model striatum
3.1 Drug therapys and sample preparation
Every day early completes each group of rat oral gavage 9 point ~ morning 10.The wherein normal saline of blank group and model control group gavage 4mL/kg; The Chinese medicine composition plus-minus taste soup of Chinese medicine composition plus-minus taste group gavage 3g crude drug/mL, 4mL/kg; Risperidone group gives the Risperidone Oral Solution of 0.2mg/4mL/kg, continuous 4 weeks.
Terminate administration next day, rat is put to death, and broken end gets brain, striatum is isolated in ice face, puts in cryopreservation tube freezing immediately, accurately weigh after solidification.Getting cerebral tissue sample 100mg adds in 5% sulfosalicylic acid of 1ml pre-cooling, homogenate under ice bath, 4 DEG C, the centrifugal 15min of 14000 × g, and get supernatant nuclepore membrane filter and filter, filtrate puts into-70 DEG C of refrigerator storage as testing sample.
3.2 dopamine (DA) and metabolite (DOPA, HVA) assay thereof
Sample preparation: ice platform is got striatum on the right side of rat, after scales/electronic balance weighing, get cerebral tissue sample 100mg and add 5% ice-cold sulfosalicylic acid of 1mL, with electric homogenate machine homogenate 1min, the centrifugal 15min of low-temperature and high-speed (4 DEG C, 4000 turns/min), take out supernatant, after 0.45 μm of membrane filtration, efficient liquid phase-electrochemical detector is adopted to measure, sampling volume 20ul.
Chromatographic condition: mobile phase buffer is 20mmol/L (trisodium citrate, being adjusted to PH with hydrochloric acid is 4.50, through the membrane filtration of 0.45um, add the methanol of 5% (v/v) after filtration, through ultrasonic degas before application, flow velocity is 1.0mL/min, column temperature remains on 35 DEG C, action potential 0.75 volt, with peak area, reference standard curve is quantitative.
Measurement result: Chinese medicine composition is added and subtracted taste soup group and risperidone group dopamine and metabolite DOPA level thereof and significantly reduced (P<0.05); And the metabolite HVA content of Chinese medicine composition plus-minus taste soup group dopamine also has remarkable reduction, significant difference (P<0.05) is had compared with model group, and its content of risperidone group is without significant change (P>0.05), the results are shown in Table 2-1.
The content of table 2-1 Dopamine In Striatum and metabolite thereof
| Group | Number of elements | DA(μg/g) | DOPA(μg/g) | HVA(μg/g) |
| Matched group | 13 | 1.53±0.78 | 0.66±0.34 | 0.35±0.19 |
| Model group | 13 | 3.80±1.72 * | 1.74±0.91 * | 0.67±0.23 * |
| Chinese medicine composition plus-minus taste group | 12 | 1.94±0.78 | 0.93±0.42 | 0.40±0.16 |
| Risperidone group | 10 | 1.66±0.69 | 0.91±0.35 | 0.56±0.27 * |
Note:
*p<0.05 compared with matched group
3.3 serotonins (5-HT) and metabolite (5-HIAA) assay thereof
Sample preparation: striatum ice platform being got rats with bilateral about 2/3, after scales/electronic balance weighing, every 100mg cerebral tissue adds the tissue homogenate of 1ml pre-cooling, in ice bath homogenate, 4 DEG C, the centrifugal 15min of 14000 × g, gets supernatant, efficient liquid phase-electrochemical detector is adopted to measure, direct injected 20ul.
Chromatographic condition: mobile phase is citric acid 63.51mmol/L, trisodium citrate 69.40mol/L, 12 sodium heptanesulfonate 1.3mmol/L, disodiumedetate 0.13mmol/L, the buffer of configuration such as methanol 16% grade, through ultrasonic degas before application, flow velocity: 0.8ml/min; Sample size: 20 μ l; Column temperature: 30 DEG C.4 road electromotive forces are :-50,100,300,500m, reference standard curve is quantitative.
Measurement result: the intrastriatal serotonin of model group rats and metabolite thereof are higher than normal group (P<0.05), the serotonin of Chinese medicine composition plus-minus taste soup group and risperidone group is starkly lower than model group rats (P<0.05), with matched group content no difference of science of statistics (P>0.05).But its metabolite is not down to normal group level, have significant difference (P > 0.05) compared with matched group, measurement result is in Table 2-2.
The content of serotonin and metabolite thereof in table 2-2 striatum
Note: * compared with matched group, P<0.05; # compares with model group, P<0.05
Chinese medicine composition plus-minus taste can regulate the content of DA, DOPA, HVA, 5-HT in striatum.Its antischizophrenic mechanism may be relevant with intrastriatal DA, DOPA, HVA, 5-HT and 5-HIAA changes of contents.
4, clinical treatment example
Model case one. Liu, man, 21 years old.
Patient, being admitted to hospital, half a year cause is not admitted to and is can not find work again, and become depressed gradually, feel oneself inferior, disheartened, animosity father and mother, humiliate its brother (its brother is educated in the university).Smell father and mother to put " poison " in his meal soup.Think that people around discusses him and " do not have ability ", ear can hear that someone talks " scold him " in the air.Deny ill, say it oneself is " very imperial emperor ".There is following schizoid cardinal symptom: killed delusion of relation, olfactory hallucination, auditory hallucination, insight is poor.Have employed haloperidol the last week and chlorpromazine is treated.Patient smells father and mother to disappear to putting " poison " in meal, and all the other symptoms have no improvement, and second week starts to take Chinese medicine composition of the present invention, and after 4 days, killed delusion of relation disappears; After 11 days, former auditory hallucination major part disappears, and sb.'s illness took a favorable turn; After 18 days, symptom is greatly taken on a new look; Be in hospital after 26 days, insight recovers completely, normal.Family members require to leave hospital.Later stage follows up a case by regular visits to, and adhere to after patient discharge taking medicine, disease does not occur again.
Model case two. Cai, female, 28 years old.
Patient, had an attack of one's old illness first in May, 1992, before once respectively at 1993,1997,2000 because schizophrenia is hospitalized for treatment.When calendar year 2001 the 4th time is in hospital, start to occur that suspection has people's evil oneself without obvious inducement, think TV and wireless content all relevant with oneself, hear there is people and oneself speaking without foundation, often refer to phenomenon at one's side, irritability with " spirit is different ", severe with family relationship, insomnia.This time previous moon other drug treatment in hospital, psychotic symptoms has no obvious improvement, being in hospital the 41st day, starts to take Chinese medicine composition of the present invention.After treatment in 25 days, patient's private prosecution auditory hallucination, vain hope disappear; Drug composition treats the 34th day in the present invention, and mental symptom major part disappears; At the 41st day, patient recovered normally substantially, and family members handle and leave hospital.Later stage follows up a case by regular visits to, and adhere to after patient discharge taking medicine, disease does not occur again.
Model case three. Cui, man, 22 years old
Patient is paranoid schizophrenia after diagnosing, before being in hospital, has fallen ill 1 year, is suspicious of large, flies into a rage, scolding father and mother.Thinking that others speaks is all say oneself.Once saw that on wall, a width is drawn, and said that is that Jiang Zi tooth is said " Military Science of Sun Zi " to oneself, has idea of reference, have auditory hallucination at ancient temple; Patient's apathetic expression, life sluggishness simultaneously, hypobulia, does not work, without insight.After being admitted to hospital, mainly first carry out simple western medicine, through treatment in two weeks, have no obvious improvement.Therefore physician adds and used traditional Chinese medicine composition for treating of the present invention, through 1 week medication patient Clear consciousness, contact is good, and thinking is smooth, and it is clear to nurse one's health; True property auditory hallucination disappears, and insight recovers to some extent.Treatment the 18th day, patient's cardinal symptom was eliminated substantially, and family members require to go home to continue to take medicine, therefore band medicine is left hospital, and is not in hospital so far again.
Model case four. Yuan, man, 36 years old
Patient is without obvious cause gradually onset before 6 years, and main manifestations is that paroxysmal says magic ghost, and embrace and beat injustice, speech increases, and the length of one's sleep is few, full of vitality, is at will made a gift to someone by money, overstates and oneself can manage it, and others is not as oneself.Once crossed twice at Shanghai hospitalization, be diagnosed as " mania ".Be in hospital first three sky, have an attack of one's old illness without reason, speech increases, time and Laden, time and Chen Shui-bian, cannot effectively talk, association flight, without insight.Within 10th, bring into use traditional Chinese medicine composition for treating of the present invention in hospital, symptom is eliminated very fast.Be in hospital the 25th, stable disease, partner treatment, with surrounding patient in, thinking is smooth, and talk orderliness is clear, and insight is still poor.Be in hospital the 32nd day, recovery from illness is left hospital.
Model case five. Ni, female, 47 years old
Patient before 11 years because operating pressure is large, onset gradually.Main manifestations is malaise, hears the news without foundation, and half goes to the Ministry of Public Security such as distant place at night sends someone to meet him.Say that Jiang Zemin calls him up, make him go to Beijing to work, think that oneself is monitored by other people, often speakes to oneself.When being admitted to hospital, be diagnosed as paranoid schizophrenia.Second day, be reluctant that cooperation is taken medicine, say and oneself practiced world merit, saved the whole of China.Within 4th, bring into use traditional Chinese medicine composition for treating of the present invention in hospital.9th, flat affect, without insight, still said what someone said before some.16th, conditions of patients slightly took a turn for the better, and active exposure is good, auditory hallucination transference cure, did not practice world merit again, but will around thing disorderly pull on one's body.23rd, the photis of patient, delusion of relation, delusion of observation disappeared, and thing said before probably knowing does not exist, and insight does not recover completely.Be admitted to hospital (using traditional Chinese medicine composition for treating of the present invention 37th) on the 41st, patient mental's symptom disappears completely, and band medicine is left hospital, and adheres to periodic review always.
Claims (3)
1. be used for the treatment of a schizoid Chinese medicine composition, it is characterized in that, in mass fraction, comprise the crude drug of following component:
Radix Gentianae 90 parts, Radix Bupleuri 45 parts, 90 parts, the Radix Rehmanniae, Magnetitum 240 parts, Radix Salviae Miltiorrhizae 30 parts, Semen Ziziphi Spinosae (parched) 90 parts, the Radix Angelicae Dahuricae 30 parts, 50 parts, Poria, Ramulus Uncariae Cum Uncis 30 parts, Rhizoma Dioscoreae 90 parts, Semen Myristicae 45 parts and Fructus Ligustri Lucidi 30 parts.
2. be used for the treatment of schizoid Chinese medicine composition as claimed in claim 1, it is characterized in that, after each taste crude drug is pressed proportion by weight, decocting with water to its volume concentration is 1/4 ~ 1/5, obtains the extract decocted, directly takes;
Or using the extract decocted as effective ingredient, add or do not add adjuvant and make pill, capsule, tablet, powder, granule, decoction or oral liquid.
3. be according to claim 1ly used for the treatment of schizoid Chinese medicine composition in preparation for the application of schizoid medicine; Or the application of the medicine of neurotransmitter is regulated in preparation.
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| CN103736041B (en) * | 2013-12-14 | 2015-11-18 | 郑明立 | One treats schizoid medicine and preparation method thereof |
| CN105833089A (en) * | 2016-05-18 | 2016-08-10 | 孙新永 | Traditional Chinese medicine composition for treating schizophrenia |
| CN111184795A (en) * | 2020-02-27 | 2020-05-22 | 西安市精神卫生中心 | Traditional Chinese medicine composition for treating cognitive function impairment, decoction based on traditional Chinese medicine composition and preparation method |
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| CN101411798A (en) * | 2008-12-02 | 2009-04-22 | 贵州益铭中医药科学技术研究所 | Chinese medicine preparation for treating schizophrenia |
| CN101979039A (en) * | 2010-10-26 | 2011-02-23 | 山西省长治精神病院 | Medicine for treating mental disease |
| CN102114229A (en) * | 2011-02-24 | 2011-07-06 | 张岚 | Traditional Chinese medicine preparation for treating schizophrenia |
| CN102512633A (en) * | 2011-12-23 | 2012-06-27 | 荣成市崖头美全口腔诊所 | Traditional Chinese medicinal composition for treating insomnia |
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| CN101411798A (en) * | 2008-12-02 | 2009-04-22 | 贵州益铭中医药科学技术研究所 | Chinese medicine preparation for treating schizophrenia |
| CN101979039A (en) * | 2010-10-26 | 2011-02-23 | 山西省长治精神病院 | Medicine for treating mental disease |
| CN102114229A (en) * | 2011-02-24 | 2011-07-06 | 张岚 | Traditional Chinese medicine preparation for treating schizophrenia |
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