CN102771791A

CN102771791A - Healthcare food and preparation method and application thereof

Info

Publication number: CN102771791A
Application number: CN2012102877644A
Authority: CN
Inventors: 覃仁安; 李楚源; 张晓燕
Original assignee: GUANGZHOU BAIYUNSHAN HEJI HUANGPU CHINESE MEDICINE CO Ltd
Current assignee: GUANGZHOU BAIYUNSHAN HEJI HUANGPU CHINESE MEDICINE CO Ltd
Priority date: 2012-08-13
Filing date: 2012-08-13
Publication date: 2012-11-14

Abstract

The invention discloses healthcare food capable of improving cardiovascular and cerebrovascular circulation systems, increasing the release of NO in vascular endothelial cells and enhancing the anoxia resistance of the organism, a preparation method thereof and application of the healthcare food in preventing cardiovascular and cerebrovascular diseases and improving the anoxia resistance of the organism. The healthcare food disclosed by the invention is prepared from the following raw materials in parts by weight: 1-6 parts of American ginseng, 8-16 parts of radix salviae miltiorrhizae and 1-5 parts of panax pseudoginseng. The preparation method of the healthcare food disclosed by the invention completely extracts the active ingredients of radix salviae miltiorrhizae; and meanwhile, by adopting a micronization technology for the panax pseudoginseng and radix salviae miltiorrhizae, the surface area of the healthcare food is increased, the absorbance is improved, and the damage to the active ingredients of the panax pseudoginseng and radix salviae miltiorrhizae in the extraction process is reduced so as to improve the healthcare effect.

Description

A kind of health food and preparation method thereof and uses thereof

Technical field

The present invention relates to the health food technology field, relate in particular to a kind of health food and preparation method thereof and reach application at aspects such as prevention cardiovascular and cerebrovascular disease and raising body anoxia endurances.

Background technology

DANQI PIAN records in first in ministry standard Chinese traditional patent formulation preparation, and writing out a prescription is: red sage root 150g, pseudo-ginseng 150g.Technology is: Radix Notoginseng powder is broken into segmentation; Red sage root boiling three times, collecting decoction filters, and filtrate decompression is concentrated into the clear cream of relative density 1.35-1.40 (50 ℃); 1 part of qinghuo reagent adds 1 part of pseudo-ginseng fine powder, and mixing is dry, is pressed into 600, or sugar coating again, promptly gets.This DANQI PIAN function cures mainly stagnation of QI and blood, ambition paralysis, dizziness and headache, the bellyache in menstrual period of being used for promoting blood circulation and removing blood stasis.DANQI PIAN in the prior art extracts effective component in red sage owing to adopt water to put forward mode in the manufacturing process flow, makes that accounting for the very big liposoluble constituent of effective component in red sage proportion does not obtain extracting; And DANQI PIAN is owing to only comprising the red sage root and pseudo-ginseng, so function singleness only has the microcirculatory function of improvement.

Summary of the invention

In view of this, the object of the present invention is to provide a kind of health food and preparation method thereof, this health food has the release that improves the cardiovascular and cerebrovascular circulatory system, increases NO in the vascular endothelial cell, improves multiple functions such as body anoxia endurance.The present invention also provides the purposes of this kind health food at medicines such as preparation treatment diabetic complication, osteoporosis, senile dementia, cardiovascular and cerebrovascular diseases.

For solving the problems of the technologies described above, technical scheme of the present invention is:

A kind of health food is processed by the raw material of following weight portion: 1～6 part of American Ginseng, 8～16 parts of the reds sage root, 1～5 part of pseudo-ginseng.

Preferably, said health food is processed by the raw material of following weight portion: 2～5 parts of American Ginsengs, 9～15 parts of the reds sage root, 2～4 parts of pseudo-ginseng.

Preferably, said health food is processed by the raw material of following weight portion: the raw material by following weight portion is processed: 2 parts of American Ginsengs, 14 parts of the reds sage root, 4 parts of pseudo-ginseng.

A kind of preparation method of above-mentioned health food comprises the steps:

1) adopts CO ₂Supercritical fluid extracts the red sage root or adopts ethanol from red rooted salvia, to extract the red sage root from red rooted salvia, obtain red sage root concentrate;

2) micropowders is processed in pseudo-ginseng, American Ginseng pulverizing, mixed, process the Chinese medicine composition of fine-powder with red sage root concentrate;

3) in step 2) in Chinese medicine composition in add appropriate amount of auxiliary materials, make.

Preferably, described preparation method in the step 1), adopts CO ₂The process of the supercritical fluid extraction red sage root is:

1a) with 850mLL ^-1～1000mLL ^-1Ethanol is entrainer, under 18MPa～22MPa, 40 ℃～50 ℃ condition, and extraction red rooted salvia 0.8h～1.5h;

1b) again under 28MPa～32MPa, 60 ℃～70 ℃ condition, with 80mLL ^-1～120mLL ^-1Ethanol be entrainer extraction 1.5h～2.5h, concentrate, the residue water extracts, and the extract that obtains is mixed with concentrate, must red sage root concentrate.

Preferably, described preparation method, in the step 1), the process of the alcohol extract red sage root is:

1a ') in red rooted salvia, add the ethanol of concentration 90%-98%, reflux 1～2h filters extract, and filtrate recycling ethanol also is concentrated into an amount of, subsequent use;

1b ') to add concentration be 45%～55% ethanol to the dregs of a decoction, reflux 1～2h, and extract filters, and filtrate recycling ethanol also is concentrated into an amount of, subsequent use;

1c ') dregs of a decoction boiling 1.5～2.5h, decocting liquid filters, and filtrating is concentrated into an amount of.

The application of the health food that a kind of aforementioned health food or aforementioned preparation method make aspect preparation preparation raising body anoxia endurance.

The application of the health food that a kind of aforementioned health food or aforementioned preparation method make in the diabetes complicated disease drug of preparation treatment.

The application of the health food that a kind of aforementioned health food or aforementioned preparation method make in preparation Prevention and Treatment of Osteoporosis medicine.

The application of the health food that a kind of aforementioned health food or aforementioned preparation method make in preparation treatment nephrotic syndrome medicine.

The application of the health food that a kind of aforementioned health food or aforementioned preparation method make in the nervous lesion disease drug that preparation treatment chronic cerebral ischemia causes.

The application of the health food that a kind of aforementioned health food or aforementioned preparation method make in preparation old failure of memory of control or senile dementia disease drug.

The application of the health food that a kind of aforementioned health food or aforementioned preparation method make in preparation treatment cardiovascular and cerebrovascular diseases medicament.

The health food that a kind of aforementioned health food or aforementioned preparation method make improves the application in the sleep type medicine in preparation.

The health food that a kind of aforementioned health food or aforementioned preparation method make improves the application in the old memory medicine in preparation.

The application of the health food that a kind of aforementioned health food or aforementioned preparation method make aspect preparation raising body anoxia endurance.

Compared with prior art; Health food of the present invention; Its health care is clear and definite and cheap, except improving microcirculation, can also be used for improving the cardiovascular and cerebrovascular circulatory system, improves the body anoxia endurance and prevent cardiovascular and cerebrovascular diseases, increase the release of vascular endothelial cell NO; Auxiliary hyperglycemic, improve renal function and improve learning and memory function, can be used for treating cardiovascular and cerebrovascular disease, diabetes set out disease, nephrotic syndrome and old failure of memory etc.Through the document retrieval, do not find to have the application of this functional health care food at present as yet.The red sage root that the present invention is used and pseudo-ginseng all are the traditional materials that not only can do medicine but also can cook health products, few side effects, and aboundresources, manufacture craft is simple and easy, and is stable and controllable for quality.The preparation method of health food provided by the invention; Can make the extracts active ingredients of the red sage root complete; Simultaneously pseudo-ginseng and American Ginseng are adopted micronization processes, can increase the surface area of health food, improve its trap; And be reduced in the leaching process destruction, thereby improve curative effect to pseudo-ginseng and American Ginseng active ingredient.

The specific embodiment

In order to make those skilled in the art understand technical scheme of the present invention better, through specific embodiment the present invention is done further detailed description below.

Embodiment one

The health food of present embodiment (being called the American ginseng DANQI PIAN) is processed by following component: American Ginseng 50 grams, the red sage root 350 grams, pseudo-ginseng 100 grams, process 1000 altogether.

Method for making: adopt CO ₂Supercritical fluid extracts the red sage root from red rooted salvia, promptly with 950mLL ^-1Ethanol is entrainer, and under 20MPa, 45 ℃ of conditions, extraction red rooted salvia 1h is again under 30MPa, 65 ℃ condition, with 100mLL ^-1Ethanol be entrainer extraction 2h, obtain red sage root concentrate, residue water extracts.Pseudo-ginseng, ginseng powder are broken into fine powder (or ultra micro powder), process the Chinese medicine composition of fine-powder with above-mentioned red sage root concentrate, add appropriate amount of auxiliary materials, mixing, and drying, compress tablet coating is processed lozenge or encapsulated, promptly gets.

In other embodiments, also can 850mLL ^-1～1000mLL ^-1Ethanol be entrainer, like 900mLL ^-1Ethanol, 850mLL ^-1Ethanol, 920mLL ^-1Ethanol, 980mLL ^-1Ethanol, 1000mLL ^-1Ethanol.The condition of the extraction red sage root can be 18MPa～22MPa, 40 ℃～50 ℃, 0.8h～1.5h, like 18MPa, 50 ℃, 1.5h or 19MPa, 48 ℃, 1.2h or 22MPa, 40 ℃, 0.8h.The condition of extraction can be once more:

Under 28MPa～32MPa, 60 ℃～70 ℃ condition, with 80mLL ^-1～120mLL ^-1Ethanol be entrainer extraction 1.5h～2.5h, like 28MPa, 70 ℃, 1.5h or 29MPa, 65 ℃, 1.8h or 32MPa, 60 ℃, 2h, the 80mLL that entrainer can be ^-1Ethanol, 85mLL ^-1Ethanol, 92mLL ^-1Ethanol, 98mLL ^-1Ethanol, 110mLL ^-1Ethanol, 120mLL ^-1Ethanol.

Present embodiment adopts supercritical extraction technique to extract the active ingredient of the red sage root; Make that accounting for the very big liposoluble constituent of effective component in red sage proportion is able to extract; Add American Ginseng simultaneously, make product in the microcirculatory effect that has supplementing qi and nourishing yin, the intelligence development of allaying excitement, clearing heat and promoting fluid, enhance immunity simultaneously of original improvement.The present invention has following effect through the zoopery proof:

1, improves heart and brain blood circulation disorder, increase coronary flow, increase the brain blood flow, promote microcirculation, increase the release of NO in the blood vessel endothelium, reduce vascular resistence, increase myocardium oxygen utilization effect, increase blood vessel elasticity, regulate blood pressure, improve the body anoxia endurance.

2, adjusting maincenter, alleviating fatigue is improved renal function and can be used for diseases such as nephrotic syndrome;

3, coronary heart disease ischemical reperfusion injury model there is the significant protection effect; Protection cardiac muscle cell's during ischemia-reperfusion SOD vigor reduces the generation of MDA, and the release of CPK and LDH is had the obvious suppression effect; And can dwindle infarct size and can reduce heart stalk area; Can significantly improve serum NO level behind the rat myocardial ischemia and reperfusion, thereby the protection cardiac muscle cell avoids ischemic lesions with vascular endothelial cell, have the effect of protecting ischemic myocardium;

4, improve sleep, enhance memory ability, senile dementia is had remarkable efficacy;

5, can regulate secretion, the auxiliary hyperglycemic of pancreas islet, therefore powerful to diabetes;

6, increase bone density, be used to prevent and treat osteoporosis;

7, alleviate the nervous lesion that chronic cerebral ischemia causes, protection brain tissue etc.

Below be related experiment:

One, gained American ginseng DANQI PIAN of the present invention improves the experimental study of body anoxia endurance

Along with development of market economy, social life rhythm is accelerated, and competing day by day increases, and strengthens the human quality, eliminates fatigue rapidly, keeps vigorous energy to become needs of people.

This research is with reference to Ministry of Public Health's health food functional evaluation method, and content comprises: the anti-anoxic experiment of normal pressure, natrium nitrosum are poisoned and are tested and the experiment of acute cerebral ischemia anoxic.Be intended to inquire into and explore the American ginseng DANQI PIAN, for the further developmental research of American ginseng DANQI PIAN provides scientific basis in the effect that improves aspect the body hypoxia-bearing.

One, materials and methods

1. material

1.1 medicine

The American ginseng DANQI PIAN, Guangzhou Baiyunshan Heji Huangpu Chinese Medicine Co., Ltd. produces, lot number 1004021; DANQI PIAN, Shanxi Rdragon Pharmaceutical Group Co., Ltd. produces, lot number 1004022.

1.2 animal

150 of healthy male Kunming mouses.Body weight 18-22g.Zhongshan University's Experimental Animal Center, the quality certification number: 2011A045.

2. experimental technique

2.1 with reference to Ministry of Public Health's file, defend prison and send out (1998) No. 23 " the health food function assessment assessment process and the method for inspection ", content comprises mouse normal pressure hypoxia endurance test, natrium nitrosum poisoning survival test and acute cerebral ischemia hypoxia test.T check between product test method employing group.

2.2 the anti-anoxic experiment of mouse normal pressure

Get and tried 50 of mouse, be divided into 5 groups at random, be respectively control group, the high, medium and low dose groups of American ginseng DANQI PIAN and DANQI PIAN group.Experimental group is irritated stomach 0.6g/kg respectively every day, 1.2g/kg, the American ginseng DANQI PIAN of 2.4g/kg.Control group then gives Isodose distilled water.The DANQI PIAN group gives the DANQI PIAN of g/kg.1 time/d, continuous irrigation stomach 30d, and carry out routine and raise after last gives 1h, with in the 250ml wide-mouth bottle that taken in respectively by examination group mouse to fill the 10g soda lime (1 every bottle), seals bottleneck with vaseline.Timing immediately is an index with the breath stopped, respectively receives the time-to-live of examination group mouse.

The survival experiment 2.3 natrium nitrosum is poisoned

Get and tried 50 of mouse, be divided into 5 groups at random, be respectively control group, the high, medium and low dose groups of American ginseng DANQI PIAN and DANQI PIAN group.Experimental group is irritated stomach 0.6g/kg respectively every day, 1.2g/kg, the American ginseng DANQI PIAN of 2.4g/kg.Control group then gives Isodose distilled water.The DANQI PIAN group gives the DANQI PIAN of 2.1g/kg.1 time/d, continuous irrigation stomach 30d, and carry out routine and raise, after last gives 1h, each treated animal lumbar injection natrium nitrosum 240mg/kg, timing immediately, the time-to-live that record is respectively tried mouse.

2.4 acute cerebral ischemia anoxic experiment

Get and tried 50 of mouse, be divided into 5 groups at random, be respectively control group, the high, medium and low dose groups of American ginseng DANQI PIAN and DANQI PIAN group.Experimental group is irritated stomach 0.6g/kg respectively every day, 1.2g/kg, the American ginseng DANQI PIAN of 2.4g/kg.Control group then gives Isodose distilled water.The DANQI PIAN group gives the DANQI PIAN of 2.1g/kg.1 time/d, continuous irrigation stomach 30d, and carry out routine and raise, after last gave 1h, each treated animal by only breaking end, was pressed stopwatch from neck immediately, write down the time and the mouth breathing number of times of each treated animal broken end back to breath stopped.

2.5 statistical method

Statistical procedures; All data all adopt mean ± standard deviation expression; Analyze with the SPSS statistical software; Relatively use variance analysis between group, significant difference is checked with q.

Two, result

1, the anti-anoxic time-to-live of mouse normal pressure

Compare with the blank group, seven each dose groups of red American ginseng all can raising anoxia in mice condition in various degree under mean survival time, red seven groups prolong 5.7%; American ginseng DANQI PIAN low dose group prolongs 6.2%; Middle dose groups prolongs 20.73%, and high dose group prolongs 29.47%, statistical procedures; The American ginseng DANQI PIAN is high, middle group compare with the blank group, difference has statistical significance (P＜O.01).There was no significant difference between DANQI PIAN group, American ginseng DANQI PIAN low dose group and the blank control group.Prompting American ginseng DANQI PIAN has humidification to the anoxia endurance of mouse, and middle and high dose groups curative effect obviously is superior to DANQI PIAN group (p＜0.05, p＜O.01).

Table 1 American ginseng DANQI PIAN is to the influence

of mouse hypoxia-bearing capability

Annotate: with red seven groups of comparisons ^△P＜0.05, ^{△ △}* p＜0.05, * * p＜0.01 are compared with blank control group in p＜0.01.

2, natrium nitrosum poisoning survival experiment

Compare with the blank group, the raising mouse that each dose groups of American ginseng DANQI PIAN all can be in various degree is at the natrium nitrosum back mean survival time of poisoning, and red seven groups prolong 6.9%; American ginseng DANQI PIAN low dose group prolongs 13.3%; Middle dose groups prolongs 31.2%, and high dose group prolongs 38.2%, statistical procedures; The American ginseng DANQI PIAN is high, middle group compare with the blank group, difference has highly significant property meaning (P＜0.01).Significant difference (P＜0.05) is arranged between DANQI PIAN group, American ginseng DANQI PIAN low dose group and the blank control group.Prompting American ginseng DANQI PIAN has humidification to the survival of mouse after natrium nitrosum is poisoned, and middle and high dose groups curative effect obviously is superior to DANQI PIAN group (p＜0.05, p＜0.01).

Table 2 American ginseng DANQI PIAN is to the influence

of poisoning time-to-live of mouse natrium nitrosum

3, acute cerebral ischemia anoxic experiment

Compare with the blank group, the raising mouse broken end back breathing time that each dose groups of American ginseng DANQI PIAN all can be in various degree, red seven groups prolong 5.6%; American ginseng DANQI PIAN low dose group prolongs 6.1%; Middle dose groups prolongs 17.3%, and high dose group prolongs 24.5%, statistical procedures; The American ginseng DANQI PIAN is high, middle group compare with the blank group, difference has highly significant property meaning (P＜0.01).Significant difference (P＜0.05) is arranged between DANQI PIAN group, American ginseng DANQI PIAN low dose group and the blank control group.Prompting American ginseng DANQI PIAN can improve the breathing time of chmice acute cerebral hypoxia ischemia, and middle and high dose groups curative effect obviously is superior to DANQI PIAN group (p＜0.01).

Table 3 American ginseng DANQI PIAN is to the influence

of chmice acute cerebral hypoxia ischemia time

Figure 2012102877644100002DEST_PATH_IMAGE001

Figure 2012102877644100002DEST_PATH_IMAGE002

Three, sum up

Health food of the present invention-American ginseng DANQI PIAN is to excavate, summing up on the basis of traditional Chinese medicine prevention cardiovascular and cerebrovascular disease experience, in conjunction with the achievement in research of modern medicine, pharmacology of Chinese materia medica.This Project Study mainly is to observe the resisting oxygen lack of American ginseng DANQI PIAN to mouse.Experimental result shows: use each dose groups of American ginseng DANQI PIAN all can raising anoxia in mice condition in various degree under mean survival time, high, the middle group time-to-live, difference had highly significant property meaning apparently higher than the blank group.In the mouse natrium nitrosum poisoning survival experiment, high dose (2.4g/kg) can significantly improve the mouse natrium nitrosum and poison the back time-to-live.This possibly enough improve the cardiovascular and cerebrovascular circulatory system with the American ginseng DANQI PIAN, and it is relevant that the raising body carries the oxygen function.The American ginseng DANQI PIAN can obviously prolong dehisce after mouse breaks end snorting time and respiration rate, explains that it has extremely significantly effect to reducing the brain oxygen consumption.In sum, the American ginseng DANQI PIAN has certain resisting oxygen lack.Therefore, the American ginseng DANQI PIAN has the potential value that is developed as health products, can supply special population, and particularly the brain worker takes.

Two, gained American ginseng DANQI PIAN of the present invention is to the experimental study of ischemic myocardium protection

This research is model with the anesthetized rat myocardial ischemia-reperfusion injury, and with methods such as dyeing quantitative tissue, zymetology and biochemical pharmacologies, research American ginseng DANQI PIAN is to the protective effect of ischemic myocardium and inquire into its mechanism.

One, materials and methods

1. material

1.1 medicine

The American ginseng DANQI PIAN, Guangzhou Baiyunshan Heji Huangpu Chinese Medicine Co., Ltd. produces, lot number 1004021.DANQI PIAN, Shanxi Rdragon Pharmaceutical Group Co., Ltd. produces, lot number 1004022.

1.2 animal

80 of healthy Wistar rats, male, body weight (283 ± 36) g.Department of Health of Guangdong Province's Experimental Animal Center provides, the quality certification number: 2011A018.

2. experimental technique

2.1 the mensuration of ischemia-reperfusion cardiac muscle superoxide dismutase (SOD), MDA (MDA) and serum creatine phosphokinase (CPK)

40 of Wistar rats, be divided at random (1) physiological saline group (physiological water, 5ml/kg); (2) American ginseng DANQI PIAN low dose group (0.4g/kg); (3) dosage (0.8g/kg) in the American ginseng DANQI PIAN; (4) American ginseng DANQI PIAN high dose group (1.6g/kg) (5) red seven groups (1.5g/kg).Each treated animal is 8.Irritate stomach every day 1 time, totally 5 times, 10min after the last administration is with urethane and chloralose mixed liquor (7: 1) 0.5g/kg intraperitoneal anesthesia; Make trachea cannula, the malleation artificial respiration, chest is opened through the parasternal approach for cardiac surgery stringer in the side position; Expose heart, at the pulmonary conus left border, 2mm wears 1 No. 000 silk thread through superficial layer of myocardium under left auricle of heart; With 1 diameter 2mm, the groove shape silicone tube of long 10mm places between ligature and the cardiac muscle, ligation LADCA (LAD).Periodic monitoring limbs II lead electrocardiogram (ECG) in the experimentation is cut off silk thread and is poured into 10min again behind the 45min.Perfusion finishes to get blood from left ventricle at once again, and separation of serum is to be measured.Win heart simultaneously, get the about 0.2g of left ventricle antetheca holostrome cardiac muscle rapidly, process 5% myocardium homogenate with ice physiological saline, the centrifugal 5min of 2000r/min, supernatant press pyrogallol autoxidation method (improvement slightly) survey SOD vigor; Survey the OD value at the 532nm place by TBA reaction colorimetric method (utilizing TBA and MDA condensation to form red product); According to the regression equation C=13.92A-0.093 that with the tetraethoxypropane is the standard gained; Correlation coefficient r=0.98, P＜0.01), the growing amount of calculating MDA.Serum after the separation is surveyed the activity (each sample is all got two-tube mean value) of CPK.

2.2 ischemia-reperfusion myocardial infarct size, serum lactic dehydrogenase (SLDH) (LDH) and nitrogen oxide (NO) assay

40 of Wistar rats are divided at random: and (1) physiological saline group (physiological saline, 5ml/kg); (2) American ginseng DANQI PIAN low dose group (0.4g/kg); (3) dose groups (0.8g/kg) in the American ginseng DANQI PIAN; (4) American ginseng DANQI PIAN high dose group (1.6g/kg) (5) red seven groups (1.5g/kg).Each treated animal is 8.Irritated 1 experiment of stomach anesthesia on the same day in preceding 1 day after duodenum administration 1 time again in experiment; By preceding method ligation LAD, cut off silk thread behind the 45min and irritate 30min again, get blood at left ventricle then; Separation of serum; Put in blue (N-BT) dye liquor of chlorination nitro four nitrogen in 37 ℃ of dyeing,, account for the percentage of the heart gross area by literature method calculating infarct size with every myocardium gross area of planimeter (self-control) measurement and infarct size.Serum is measured kit with NO and is located to measure absorbance A by its method of operating at ELIASA (545nm), is calculated serum NO content according to doing calibration curve with the natrium nitrosum by standard items.Measure Serum LDH active (getting two-tube mean value) by literature method in addition.

3, relatively adopt the t check between the statistical procedures group.

Two, result

1, to the influence of rat heart muscle SOD vigor MDA content and serum CPK vigor

It is as shown in table 1: after American ginseng DANQI PIAN low dose group, middle dose groups, high dose group and DANQI PIAN group myocardial ischemia 45min irritate 10min again; Its SOD vigor is apparently higher than the physiological saline control group, and three dose groups of American ginseng DANQI PIAN and red seven groups of effects do not have significant difference; MDA generates and to be starkly lower than the physiological saline control group, difference highly significant (p＜0.01), and there were significant differences (p＜0.05, p＜0.01) for three dose groups of American ginseng DANQI PIAN and red seven groups; American ginseng DANQI PIAN low dose group, middle dose groups and high dose group all can significantly reduce the release of CPK; With physiological saline group ratio; Significant difference (p＜0.01); American ginseng DANQI PIAN low dose group acts on red seven groups does not have significant difference, but middle and high two dose groups and red seven groups of effects there were significant differences (p＜0.01).

2, to the influence of rat myocardium block scope, Serum LDH and NO content

As shown in table 1: three dose groups of American ginseng DANQI PIAN and red seven groups all can dwindle infarct size; With physiological saline group ratio; Difference highly significant (p＜0.01), with red seven groups find that relatively American ginseng DANQI PIAN low dose group do not have to indifference middle and high dose groups and red seven groups of significant differences (p＜0.05); The middle and high dose groups serum NO of American ginseng DANQI PIAN is apparently higher than physiological saline group (p＜0.01), and three dose groups of American ginseng DANQI PIAN and red seven groups effect do not have significant difference; The LDH vigor is starkly lower than physiological saline group (p＜0.05, p＜0.01), but American ginseng DANQI PIAN group and red seven groups do not have significant difference.

Table 1 is respectively organized the comparison

of the SOD of Ischemia and Reperfusion in vivo in Rats cardiac muscular tissue vigor, MDA content myocardial infarct size, serum CPK vigor, NO and LDH content influence

Annotate: compare * p＜0.05, * * p＜0.01 with the physiological saline group; With red seven groups of comparisons, ^△P＜0.05, ^{△ △}P＜0.01.

Three, discuss

This result of study shows, cardiac muscle cell's SOD vigor when the American ginseng DANQI PIAN can significantly be protected ischemia-reperfusion reduces the generation of MDA, the release of CPK and LDH is had the obvious suppression effect, and can dwindle infarct size.Show that this medicine has the significant protection effect to myocardial ischemia-reperfusion injury.This effect possibly protected endogenous oxygen free radical scavenger SOD vigor with this American ginseng DANQI PIAN, and it is relevant to the damage of myocardium lipid peroxidation to have alleviated oxygen radical.And can significantly improve serum NO level behind the rat myocardial ischemia and reperfusion; Explain that the effect of this American ginseng DANQI PIAN anti-experimental character myocardial ischemia is also relevant with the content of NO in the increase vascular endothelial cell; Thereby protection cardiac muscle cell and vascular endothelial cell are avoided ischemic lesions, and this possibly be the reason on the other hand of this American ginseng DANQI PIAN anti-experimental character myocardial ischemia.

Three, the experimental study of gained American ginseng DANQI PIAN auxiliary hyperglycemic of the present invention

The data that the cardiovascular report of China is announced show that over nearly 20 years, Chinese diabetes prevalence is doubled and redoubled, China more than 2,000 ten thousand diabetics at present, and anti-sugar amount reduction person 2,000 ten thousand, China has become global diabetic's number second big country, is only second to India.This research adopts SD male rat lumbar injection STZ to set up zoic model with hyperglycemia; American ginseng DANQI PIAN with various dose is prevented and treated; And compare research with DANQI PIAN; Be intended to inquire into the therapeutic mechanism of American ginseng DANQI PIAN auxiliary hyperglycemic, for the further developmental research of American ginseng DANQI PIAN provides scientific basis.

One, materials and methods

1. material

1.1 medicine

1.2 animal

40 of healthy male SD rats, the SPF level.Body weight 160-190g.Department of Health of Guangdong Province's Experimental Animal Center provides, the quality certification number: 2011A023.

2. experimental technique

2.1 modeling and grouping

Behind the rat fasting 24h, lumbar injection STZ (60mg/kg); Behind the ad lib 12h, water 12h is can't help in fasting, again a STZ of lumbar injection (60mg/kg).One week back survey blood sugar, blood glucose value are hyperglycemia model success animal more than 10mmol/L.Select 60 of hyperglycemia model success animals, be divided into a model control group, positive controls, red seven groups and high, medium and low three dose groups of American ginseng DANQI PIAN (difference is not more than 1.1mmol/L between group) at random.

2.2 raise and method of administration

Each group is on an empty stomach administration behind the 12h all, and dose groups gives seven pairs of ginsengs of pellet sheet of variable concentrations and (irritates stomach 0.4g/kg respectively, 0.8g/kg; 1.6g/kg the American ginseng DANQI PIAN), the distillation of blank control group and model control group equal-volume, positive controls gives DANQI PIAN (1.5g/kg); Irritate stomach amount 5mL/kg, continuously 30d.All rats are all under equal conditions fed, and freely take the photograph water, control appetite.

3. observation index

3.1 fasting blood-glucose (FBG) is measured: after the last administration; Water 5h is can't help in fasting; Rat tail vein is got blood 20 μ l and is joined in the 0.4ml protein precipitant, mixing, and room temperature leaves standstill 7min; With the centrifugal 15min of 7000r/min, the 250 μ l supernatant reference reagent box specification methods of getting are measured fasting blood-glucose content.

3.2 glucose tolerance is measured: after the last administration, water 5h is can't help in fasting, irritates stomach glucose solution 2g/ (kgbw).Irritate behind the stomach 0,0.5,1 and 2h measure blood-sugar content respectively, and by formula area under the calculated curve (area under the curve, AUC): AUC [mmol/ (hL)]=0.5A+B+C+0.5D (A, B, C, D are respectively 0,0.5,1, the blood glucose value of 2h)

3.3 statistical method

Statistical procedures; All data all adopt mean ± standard deviation

expression; Analyze with the SPSS statistical software; Relatively use variance analysis between group, significant difference is checked with q.

Two, result

The influence of table 1 pair fasting blood-glucose and glucose tolerance

Annotate: compare with normal group, ^△P＜0.05; Compare * p＜0.05, * * p＜0.01 with model group

The FBG content of model control group and glucose AUC are significantly higher than normal control group (P＜0.01).After the American ginseng DANQI PIAN is irritated stomach; Each dose groups diabetes rat FBG content reduces by 22.59%, 26.91% and 31.56% (P＜0.01) respectively than model control group; And red seven groups reduced by 21.26%; Glucose AUC reduces by 10.52%, 15.79% and 16.88% (P＜0.01) respectively, and red seven groups have been reduced by 10.33%.The glucose tolerance level that shows diabetes rat is badly damaged, and the American ginseng DANQI PIAN can improve the tolerance of diabetes rat to glucose.

Three, sum up

Blood sugar can reflect the diabetes state of an illness and control situation, and glucose tolerance can be used as auxiliary characteristics.Experimental result shows that the American ginseng DANQI PIAN can significantly reduce the fasting blood-glucose and enhancing glucose tolerance of diabetes rat, and visible American ginseng DANQI PIAN ability assist control diabetes PD has good auxiliary hyperglycemic effect.

Four, the experimental study of gained American ginseng DANQI PIAN alleviating physical fatigue of the present invention

Fatigue is the biochemical change procedure of body complex physical.Nineteen eighty-two, the 5th the international movement biochemistry meeting of holding in the U.S. with the concept definition of fatigue is: by body movement itself caused " the organism physiology process can not continue hold its function on specified level and/or can not keep predetermined exercise intensity " phenomenon that causes locomitivity to descend.Alleviating physical fatigue is meant the generation of delay fatigue or the elimination that accelerates fatigue, and the material with this type of function is claimed the alleviating physical fatigue active material.This research adopts SD male rat lumbar injection STZ to set up zoic model with hyperglycemia; American ginseng DANQI PIAN with various dose is prevented and treated; And compare research with DANQI PIAN; Be intended to inquire into the therapeutic mechanism of American ginseng DANQI PIAN auxiliary hyperglycemic, for the further developmental research of American ginseng DANQI PIAN provides scientific basis.

The index of China's " the health food function assessment assessment process and method of inspection " functional evaluation of ministry of Health of China 1996 issue comprises exercise tolerance experiment (swimming with a load attached to the body and pole-climbing) and physiological and biochemical index (blood lactic acid, serum urea nitrogen, the flesh/hepatic glycogen etc.) evaluation index as anti-fatigue effect.Except that above biochemical indicator, also can detect indexs such as blood sugar, lactic dehydrogenase, hemoglobin and phosphocreatine.

One, materials and methods

1. material

1.1 medicine

1.2 animal SPF level Kunming mouse, male and female half and half, body weight 18～22g.Zhongshan University's Experimental Animal Center, the quality certification number: 2011A045.

2, experimental technique

2.1 with reference to Ministry of Public Health's file, defend prison and send out (1998) No. 23 " the health food function assessment assessment process and the method for inspection ", content comprises that swimming with a load attached to the body test, serum urea are measured, hepatic glycogen is measured, blood lactic acid is measured.

2.2 mouse swimming with a load attached to the body test

Get and tried 50 of mouse, be divided into 5 groups at random, be respectively control group, the high, medium and low dose groups of American ginseng DANQI PIAN and DANQI PIAN group.Experimental group is irritated stomach 0.6g/kg respectively every day, 1.2g/kg, the American ginseng DANQI PIAN of 2.4g/kg.Control group then gives Isodose distilled water.The DANQI PIAN group gives the DANQI PIAN of 2.1g/kg.1 time/d, continuous irrigation stomach 30d, and carry out routine and raise, after last is tried thing 30min to mouse, place the swimming case to swim the about 30cm of the depth of water, 25 ℃ ± 1.0 ℃ of water temperatures.The load sheet lead of 5% body weight of mouse root of the tail portion.The record mouse begins the extremely dead time as swimming with a load attached to the body time (s) from swimming.

2.3 serum urea is measured

After last is tried thing 30min to mouse, be not swimming with a load attached to the body 90min in 30 ℃ the water, pull out the eyeball blood sampling behind the rest 60min in temperature.In 4 ℃ leave standstill 3h after the centrifugal 15min of 2000 commentaries on classics/min, get serum and measure serum urea with automatic clinical chemistry analyzer.

2.4 hepatic glycogen is measured

After last is tried thing 30min to mouse, put to death animal.Get liver and after the physiological saline rinsing, blot with filter paper, take by weighing liver 100mg, remaining operation is carried out hepatic glycogen by the kit specification and is measured.

2.5 blood lactic acid is measured

Last is tried behind the thing 30min from the angular vein clump 20 μ L that take a blood sample, and not bearing a heavy burden then in temperature is to stop behind the swimming 10min in 30 ℃ the water, respectively at motion back 0min and the same blood sampling 20 μ L of 20min, measures blood lactic acid, calculating blood lactic acid TG-AUC.

2.6 Data Processing in Experiment

Carry out statistical analysis with Spss 11.0 softwares.During analysis, earlier data are carried out homogeneity test of variance,, adopt one-way analysis of variance totally to compare, find differences again and to carry out comparing in twos between a plurality of dose groups and control group mean with the Dunnett method if variance is neat.If heterogeneity of variance, then initial data is carried out suitable variable conversion, satisfy homogeneity test of variance after, add up with data converted; If do not reach the neat purpose of variance yet after the variable conversion, to use rank test instead and add up, discovery is overall more variant, then adopts Tamhane ' the sT2 check that does not require homogeneity of variance to compare in twos.

Two, result

1, the American ginseng DANQI PIAN is to the influence of mouse swimming with a load attached to the body time

Table 1 American ginseng DANQI PIAN is to the influence

of mouse swimming with a load attached to the body time

Annotate: compare with normal group, ^△P＜0.05, ^{△ △}* p＜0.05, * * p＜0.01 are compared with blank control group in p＜0.01.

2. the American ginseng DANQI PIAN is to the influence of mice serum urea

Table 2 American ginseng DANQI PIAN is to the influence

of mice serum urea

Annotate: compare with normal group, ^△P＜0.05, ^{△ △}* p＜0.05, * * p＜0.01 are compared with blank control group in p＜0.01

3, the American ginseng DANQI PIAN is to the influence of Mouse Liver glycogen

Table 3 American ginseng DANQI PIAN is to the influence

of Mouse Liver glycogen

4, the American ginseng DANQI PIAN is to the influence of mouse blood lactic acid

Table 4 American ginseng DANQI PIAN is to the influence

of mouse blood lactic acid

Annotate: compare with normal group, compare * p＜0.05 with blank control group.

Three, sum up

This experimental basis " health food check with assessment technique standard " swimming with a load attached to the body experimental result is positive, and appoints the binomial index positive in the blood lactic acid, serum urea, three biochemical indicators of hepatic glycogen, promptly decidable this tried the effect that thing has anti-fatigue effect.Under this laboratory condition; Per os gives the American ginseng DANQI PIAN 30 days of mouse 0.6g/kg, 1.2g/kg, 2.4g/kg dosage; 0.6g/kg dosage can obviously prolong mouse the swimming with a load attached to the body time, increase Mouse Liver glycogen storage level, reduce serum urea level behind the mouse movement, with the blank control group comparing difference statistical significance (P＜0.05) is arranged.Statistical significance (P＜0.05) is arranged with red seven groups of comparisons serum urea after increasing Mouse Liver glycogen storage level, reducing mouse movement aspect horizontal.1.2g/kg, 2.4g/kg can obviously prolong mouse the swimming with a load attached to the body time, increase Mouse Liver glycogen storage level, reduce serum urea level behind the mouse movement, with blank control group and comparing difference highly significant property statistical significance (P＜0.01) is arranged.0.6g/kg, 1.2g/kg dosage do not see that to mouse blood lactic acid TG-AUC obvious influence (P＜0.05) 2.4g/kg dosage relatively has statistical significance (P＜0.05) to mouse blood lactic acid TG-AUC and blank control group, with red seven groups do not see obvious influence.Prompting American ginseng DANQI PIAN has the function of antifatigue.

Five, gained American ginseng DANQI PIAN of the present invention improves the experimental study of sleep and auxiliary improvement learning and memory function

Along with the development of society, people's living pressure of working is increasing, and insomnia has become one of key factor of puzzlement people life.There are some researches show: the puzzlement that the whole world has 27% people to be had a sleepless night approximately.The research that improves sleep focuses mostly at aspects such as stable, epiphysins, though short-term tool certain effect is taken for a long time and can be produced dependence and toxic and side effect.The American ginseng DANQI PIAN.This experiment is intended to observe the American ginseng DANQI PIAN mouse is improved the influence of sleep function and auxiliary improvement learning and memory function, for using the American ginseng DANQI PIAN more precisely, objectively experimental basis is provided.

One, materials and methods

1. material

1.1 medicine

1.2 animal

SPF level Kunming mouse, male and female half and half, body weight 18～22g.Zhongshan University's Experimental Animal Center, the quality certification number: 2011A045.

2, experimental technique

Experimental technique is according to the method for inspection of improving sleep function and auxiliary improving memory function in Ministry of Public Health's " health food check and assessment technique standard " (version in 2003).

2.1 directly sleep test

Select 60 mouse, be divided into 3 dosage experiments groups (0.6g/kg, 1.2g/kg, 2.4g/kg), 1 negative control group and seven groups of a pellet (2.1g/kg) at random, 10 every group.Experimental group is irritated the sample solution that stomach gives above-mentioned dosage respectively, and negative control group gives the distilled water of equivalent, and irritating the stomach amount is 0.2ml/10g.BW.Give continuously and tried thing 30d, the last sample observes the sleep quality of mouse for the appearance back.Sleep is judge index with the righting reflex loss; When mouse places dorsal position, can right the body position immediately, can not the person of righting as surpassing 60s; Promptly think righting reflex loss; Get into sleep, the time of recovering from righting reflex loss to righting reflex is mouse sleep time, observes the sleep incidence of respectively organizing mouse, sleeping number of animals and the length of one's sleep.Like the sleeping number of animals of test group and increase the length of one's sleep and significant difference is arranged, judge that promptly this result of the test is positive with negative control group.

2.2 prolong the yellow Jackets test length of one's sleep

Mice group reaches to sample method with 2.1.After the last sample was given appearance 20min, each organized mouse by 40mg/kg.BW dosage lumbar injection yellow Jackets, and injection volume is 0.2ml/20g.BW.Righting reflex loss with mouse is the sleep judge index, observes and is tried thing to the prolongation effect of the length of one's sleep of yellow Jackets inducing mouse.As prolonging length of one's sleep of experimental mice and statistical significance being arranged, judge that promptly this result of the test is positive than negative control group.

2.3 barbital sodium sub-threshold dose hypnosis test

Mice group reaches to sample method with 2.1.After the last sample was given appearance 20min, each organized mouse by 150mg/k.BW dosage lumbar injection barbital sodium.Observe the sleep quality of mouse in the 30min, reaching more than the 60s with righting reflex loss is sleeping criterion, and the sleeping number of animals of each group of record is calculated sleeping animal incidence.Be higher than negative control group and statistical significance is arranged like the sleeping animal incidence of experimental group, judge that promptly this result of the test is positive.

2.4 barbital sodium sleep latency test

Mice group reaches to sample method with 2.1.Each treated animal is through lumbar injection barbital sodium 300mg/kg.BW after the last sample is given appearance 20min, and injection volume is 0.2ml/20g.BW.With the righting reflex loss is index, observes and is tried thing to preclinical influence of barbital sodium sleep.As shortening in sleep incubation period of test group mouse and statistical significance being arranged, judge that promptly this result of the test is positive than negative control group.

2.5 the result judges

If incubation period is tested the binomial positive in three experiments in the experiment length of one's sleep of prolongation yellow Jackets, barbital sodium sub-threshold dose hypnosis experiment, barbital sodium sleep, and does not have obviously direct syngignoscism, this given the test agent of decidable has the effect of the sleep function of improvement.

2.6 diving tower experiment

Mouse is placed on by reagent thing 30d to adapt to 3min in the reaction chamber; Logical immediately 36V alternating current stimulates mouse then mouse to be placed on the insulated platform, writes down each mouse and jumps off number of times that is shocked by electricity in the incubation period, 5min of platform and the number of animals of jumping off platform for the 1st time.The incubation period of jumping off platform for the 1st time, the errors number of 3min introskip lower platform and the number of animals that each group is jumped off platform are write down in retest behind the 24h.Stop to train the experiment of disappearing behind the 5d.The experiment repetition is verified for 1 time.

2.7 keep away dark experiment

Mouse receives to keep away dark experiment behind the reagent thing 30d.Write down every mouse and get into the errors number in darkroom and the number of animals that each group gets into the darkroom to getting into shocked by electricity required time (incubation period), 5min of darkroom from putting into bright chamber.The same time tests again behind the 24h, writes down every mouse and gets into and get into the number of times of darkroom mistake and number of animals that each group gets into the darkroom in incubation period, 5m in darkroom and stop to train the experiment of disappearing behind the 5d.The experiment repetition is verified for 1 time.

2.8 the American ginseng DANQI PIAN is to the influence of Alzheimer's Disease Rats learning memory disorder

2.8.1 modeling and administration

Get 50 of SD rats, male and female half and half.Reserve 9 as operative control group, all the other rats by intraperitoneal injection D-galactolipin 48mg.kg ^-1.d ^-1, continuous 6 weeks, began in the 7th week being fixed on behind the rat anesthesia on the stereotaxic instrument, skin of vertex is made the perpendicular otch in center, exposes parietal bone, behind the coronal suture of both sides, gets out aperture.The elements of a fix: 0.9mm behind the bregma, center line outside 2.6mm, dark 7.5mm, every side is annotated people's soup 1 μ l (including IBO5 μ g/ μ l) respectively with micro syringe, speed 0.2 μ l/min, let the acupuncture needle remain at a certain point 10min, operative control group is given equivalent physiological saline by same operation.The postoperative skin suture, intramuscular injection penicillin is anti-infective in 3 days.41 animals of postoperative survival are divided into 4 groups at random, add 9 animals of reservation, it is 6 groups that experiment is divided into, and each organizes continuous irrigation stomach 2 months.

2.8.2 behaviouristics experiment

The reference literature method is kept away dark experiment, and the test rat reaches medium-term and long-term study, memory capability in the recent period.Keep away the long 60cm of camera bellows, wide 25Cm, high 30cm, clearly demarcated, dark two Room.It is the round hole of 8cm that one diameter is arranged between two Room, and about 40cm place hangs a 40W tengsten lamp.All spread with the copper grid bottom, two Room, and the copper grid of bottom, darkroom can lead to the electric current of 36V, bottom, bright chamber no power.During experiment the rat face is put the bright chamber of people in the hole dorsad, start timer simultaneously.After animal passes the hole and enters the people darkroom, shocked by electricity 1 second, clocking stops automatically.Take out rat, write down every mouse and run into the required time of electric shock from putting the bright chamber of people to entering the people darkroom, this is incubation period.Number of shocks in the incubation period that the test of reforming after 1 week, record get into the darkroom and the 5min and each organize the mouse number in people darkroom.

2.9 experimental data statistics application SPSS 11.0 statistical softwares carry out statistical disposition.

Two, result

1, direct sleep experiments

Each dosage experiments treated animal has part (1～3) animal to show as quiet and movable the minimizing, but does not have righting reflex loss person's negative control treated animal all clear-headed after giving sample, shows that this sample does not have direct sleep effect to mouse, sees table 1.

Table 1 is respectively organized the direct sleep experiments result of mouse

The influence of the mouse sleep time of 2, yellow Jackets being induced, to the influence of yellow Jackets sub-threshold dose syngignoscism and to the barbital sodium preclinical influence of sleeping

Each dose groups of American ginseng DANQI PIAN and control group relatively have prolongation trend to the yellow Jackets inducing mouse length of one's sleep, and wherein high dose group and control group comparing difference have statistical significance (P＜0.05).The sleeping rate of each dose groups animal is compared difference not statistically significant (P＞0.05) with control group.The sleep of each dose groups is all shortened than control group incubation period, and wherein the high dose group barbital sodium is slept and is starkly lower than control group (P＜0.05) (table 2) incubation period.With red seven groups relatively, the low dose group no significant difference, middle and high dosage and red seven groups have notable difference (P＜0.05, P＜0.01).

Table 2 American ginseng DANQI PIAN improves the influence

of sleep function experiment to mouse

3, the American ginseng DANQI PIAN is to the influence of mouse diving tower experiment

Each dose groups of American ginseng DANQI PIAN and control group compare, and the errors number of mouse being jumped off platform has minimizing trend, and wherein high dose group and blank control group comparing difference have statistical significance (P＜0.05), each dose groups and red seven groups of indifferences of comparing.The result is consistent with repeated experiments.Experimental result is seen table 3～4.

Table 3 American ginseng DANQI PIAN is to the influence of mouse diving tower experiment

Annotate: with red seven groups of comparisons, ^△P＜0.05, ^{△ △}* p＜0.05, * * p＜0.01 are compared with blank control group in p＜0.01.

Table 4 American ginseng DANQI PIAN is to the replica test

of the influence of mouse diving tower experiment

Annotate: with red seven groups of comparisons, ^△P＜0.05, ^{△ △}* p＜0.05, * * p＜0.01 are compared with blank control group in p＜0.01

4, the American ginseng DANQI PIAN is kept away the influence of dark experiment memory to mouse

Each dose groups of American ginseng DANQI PIAN and control group are relatively; The errors number that mouse is got into the darkroom has minimizing trend; Wherein middle dose groups and blank control group comparing difference have statistical significance (P＜0.05); High dose group and blank control group comparing difference have the statistical significance (P＜0.01) of highly significant property, and high dose group and red seven groups of comparing differences have statistical significance (P＜0.05).The result causes with repeated experiments.Experimental result is seen table 5～6.

Table 5 American ginseng DANQI PIAN is kept away the influence test

of dark experiment to mouse

Table 6 American ginseng DANQI PIAN is kept away the replica test

of the influence of dark experiment to mouse

5, to the influence of senile dementia animal memory dysfunction

First test; Each is organized the mean value in incubation period that rat enters the people darkroom and is about about 10s, and all rats all enter the people darkroom, and the model control group rat advances the number of times in people darkroom obviously more than operative control group (P＜0.01) in 5min; Show the modeling success, and this model there is higher reappearance.Advance people darkroom number of times in the American ginseng DANQI PIAN low dose group 5min and obviously be less than model control group (P＜0.05).The back test of one week; The incubation period that operative control group, the high, medium and low dose groups of American ginseng DANQI PIAN are entered the people darkroom is than the apparent in view prolongation of model control group; Advance people darkroom number of times in the 5min and obviously be less than model control group; And middle and high group of American ginseng DANQI PIAN with red seven groups relatively there were significant differences, show that Fufang Danshen Pian has tangible consolidation effect to senile dementia rat intermediate memory.The result is as shown in table 7.

Table 7 American ginseng DANQI PIAN is to the influence

of senile dementia animal memory dysfunction

Three, conclusion

This experimental basis " health food check and assessment technique standard " is given mouse stomach 30d with the American ginseng DANQI PIAN of three kinds of various dose, has carried out improving the experimental study of sleep and auxiliary improvement learning and memory function.The experiment length of one's sleep of prolongation yellow Jackets, barbital sodium sub-threshold dose hypnosis experiment, the barbital sodium that wherein improves sleep slept, and to test three experiments incubation period all positive; And do not have obviously direct syngignoscism, decidable American ginseng DANQI PIAN has the effect of the sleep function of improvement; Through the experiment of mouse diving tower and platform experiments and 2 repeated experiments, the result shows that the American ginseng DANQI PIAN of high dose and control group compare, and have the function of tangible auxiliary improvement of memory.Through the American ginseng DANQI PIAN senile dementia animal is kept away dark experiment, can find out that the American ginseng DANQI PIAN can improve the memory dysfunction of AD animal, senile dementia rat intermediate memory is had tangible consolidation effect.Therefore can draw the American ginseng DANQI PIAN has the function of improving sleep, auxiliary improvement of memory and improving senile dementia.

Six, gained American ginseng DANQI PIAN of the present invention is prevented and treated osteoporotic experimental study

One, materials and methods

1, material

(1) medicine

(2) animal selected for 12 monthly ages for use, 72 of cleaning level, SD female rats, and (390 ± 20g), Department of Health of Guangdong Province's Experimental Animal Center provides body weight, the quality certification number: 2011A011.Give normal diet and feed, with reference to AIN-93M rodent feed formula, with the composition that derives from soybean in the suitable substitution of materials feed, to avoid of the interference of plant hormone such as isoflavones experimental result.Freely take the photograph water, throw light on round the clock that the light and shade time of replacing is 12-12, adaptability was fed 7 days.

2, method

Modeling method reference literature, rat be through the anesthesia of the amobarbital sodium solution of lumbar injection 30mg/kgBW, fixing back, abdomen position in ventrimeson apart from introitus 3-4cm unhairing; Use the tincture of iodine and alcohol disinfecting respectively; Skin and the about 2-3cm of abdominal muscle are cut in dried slightly back, and it is thus clear that the otch visual field is white adipose, after pushing fat deposit aside and finding the uterus; Survey cornua uteri with one gently and pull out, terminal visible at it by the ovary of cellulite parcel.Fractionation of fatty group just can see pink ovary, clamps ovary with haemostatic clamp, then fallopian tubal under the ovary (comprising fat) is used the silk thread ligation, wipes out ovary (whether inspection wipes out fully), takes advantage of a situation cornua uteri is sent back in the abdominal cavity, wipes out other side ovary with method.After abdominal muscle and skin layering are sewed up, sterilization once more.After hindlimb muscle is injected 20,000 U penicillin.Sham-operated control group (10) is opened and is sewed up after abdomen is wiped out a fritter fat.Postoperative 5 days, animal begins the administration of dividing into groups after recovering.Be respectively sham operated rats, model group, the high, medium and low dose groups of American ginseng DANQI PIAN and DANQI PIAN group.Experimental group is irritated stomach 0.4g/kg respectively every day, 0.8g/kg, the American ginseng DANQI PIAN of 1.2g/kg.Control group and sham operated rats then give Isodose distilled water.The DANQI PIAN group gives the DANQI PIAN of 1.5g/kg.Every day gastric infusion once, all administrations 6 days, altogether 16 weeks of administration.Duration of test continues to feed and drinking-water according to normal diet.

3, observation index

(1) mensuration of index such as serum biochemistry

16 week of modeling, the back femoral artery was got blood, after blood sample is originally treated, and separation of serum, each group is got 10 samples at random and is carried out serum measured by radioimmunoassay estradiol (E2), interleukin-6 (IL-6); Complete taking-up right side femur is peeled off totally, claims weight in wet base.Separating uterus is weighed.Calculate the uterus index and the femur index of every 100g body weight.

(2) bone density

With the right side femur that takes out with Japanese NORLAND dual intensity bone density meter, scanning survey bone density and bone mineral content.

Two, result

1, changes of bone mineral density

Removal ovary is after 16 weeks, and right femoral bmd of oophorectomize group rat and bone mineral density all are starkly lower than Sham-operated control group, and difference has conspicuousness (P＜0.01), shows the modeling success.Compare with the oophorectomize group, dose groups and high dose group also can obviously improve OVX rat femur BMC and BMD content (P＜0.05, P＜0.01) in the American ginseng DANQI PIAN.With red seven groups of comparisons, basic, normal, high dosage has notable difference (P＜0.05, P＜0.01) to see table 1.

Table 1 American ginseng DANQI PIAN is to the influence of female OVX rat femur BMD, BMC

Annotate: with red seven groups of comparisons, ^△P＜0.05, ^{△ △}* p＜0.05, * * p＜0.01 are compared with control group in p＜0.01.

2, the variation of serum E2 and IL-6

Table 2 is visible, and removal ovary is after 16 weeks, and oophorectomize group rat blood serum E2 content significantly is lower than Sham-operated control group, and difference has conspicuousness (P＜0.05), shows the modeling success.Dose groups and high dose group E2 content are apparently higher than model group in the American ginseng DANQI PIAN, and difference has conspicuousness (P＜0.05), and with red seven groups notable difference (P＜0.05, P＜0.01) is relatively arranged.

Table 2. American ginseng DANQI PIAN is to the influence of female OVX rat E2 and IL-6

3, uterus exponential sum femur index

Table 3 is visible, compares with sham operated rats, and oophorectomize group uterus index and femur index obviously reduce, and have statistical significance (P＜0.01), prompting modeling success.With the oophorectomize group relatively, three dose groups uterus indexes obviously increase (P＜0.01), and middle dose groups and red seven groups have notable difference (P＜0.01); High, normal, basic three dose groups femur indexes all obviously increase ((P＜0.05, P＜0.01), and high dose group there were significant differences (P＜0.01) in red seven groups.

Table 3. American ginseng DANQI PIAN is to the influence

of female OVX rat uterus exponential sum femur index

Annotate: with red seven groups of comparisons, ^△P＜0.05, ^{△ △}* p＜0.05, * * p＜0.01 are compared with control group in p＜0.01

Three, conclusion

The result shows: the bone mineral content and the bone density of American ginseng DANQI PIAN treatment group all are higher than model control group, show the function that the American ginseng DANQI PIAN has increases bone density.Compare with model group, the American ginseng DANQI PIAN can obviously stop rat blood serum E ₂Level descends and right femur weight in wet base descends, and shows that the American ginseng DANQI PIAN has the reduction of prevention climacteric estrogen level and increases the bone density function.

The 7th part gained American ginseng of the present invention DANQI PIAN is to the protective effect of chronic cerebral ischemia rat cerebral tissue

Chronic cerebral ischemia is brought great influence because long-term cerebral blood flow (CBF) hypoperfusion can cause the ability of learning and memory obstacle to patient's life.Chronic cerebral Hypoxic Rats cognitive ability descends, and possibly cause the neure damage forfeiture that relation is arranged with chronic cerebral ischemia; Damage the signal transmission generation obstacle that causes with white matter after the chronic cerebral ischemia; The structure of cynapse and the number relation that changed.The American ginseng DANQI PIAN mainly is made up of the red sage root, pseudo-ginseng, American Ginseng etc.Have effects such as promoting blood circulation and removing blood stasis, regulating qi-flowing for relieving pain.This research adopts Longa improvement bolt collimation method to cause rat acute focal cerebral ischemia model, observes the American ginseng DANQI PIAN to acute cerebral ischemia rat result of treatment, inquires into the pharmacological action of American ginseng DANQI PIAN treatment acute cerebral ischemia rat.

One, materials and methods

1. material

(1) medicine

(2) animal

10～12 monthly ages 40 of healthy Wistar rats, body weight is 300～400g, male and female half and half, Department of Health of Guangdong Province's Experimental Animal Center provides, the quality certification number: 2011A036.

2. experimental technique

2.1 modeling and grouping

10% chloraldurate intraperitoneal injection of anesthesia, anaesthetize successfully after, neck preserved skin, sterilization; Adopt neck median incision, passivity is separated, and exposes bilateral common carotid arteries; Every side arteria carotis communis adopts No. 0 surgical thread to carry out ligation at proximal part and distal end respectively, in the middle of the ligation two ends, cuts off.The sham operated rats rat is only isolated bilateral common carotid arteries, and not ligation is not cut off.With animal be divided into Sham-operated control group, chronic cerebral ischemia group at random, red seven groups and ischemic protection are organized 4 groups, every group each 10.American ginseng DANQI PIAN group postoperative gives American ginseng DANQI PIAN 0.8g/kg next day and irritates stomach, every day 1 time, 4 weeks of successive administration.Red seven groups give 1.5g/kg and irritate stomach, Sham-operated control group and the American ginseng DANQI PIAN group distilled water that deturs talis dosis next day of performing the operation;

3. observation index

3.1 rat Cognitive Aptitude Test

Adopt Y types electricity labyrinths to measure the rats ability that initiatively escapes learning in postoperative 12 week.Rat is put into labyrinth box, be driven to 1 arm starting area after conforming, adopt electric shock to make rat escape the place of safety, escape the place of safety to rat and be decided to be correct response, otherwise be wrong reaction.Row test for the second time behind the rat rest 1min.The number of times that shocks by electricity when the record rat reaches association's standard.

3.2FAP immunohistochemical staining

Identify positive cell.GFAP is that endochylema, the projection of astroglia is painted, and positive cell is pale brown look.On each slide, get 5 high power lens visuals field,, calculate the positive cell average under each high power lens the positive cell counting number in 5 high power lenses.

3.3 statistical procedures

Adopt 14.0 couples of statistics software SPSS respectively to organize the gained data and carry out statistical analysis, adopt mean ± standard deviation to represent, relatively adopt one-way analysis of variance between many groups, relatively adopt the q check between group, P＜0.05 shows that difference has statistical significance.

Two, result

2.1 four groups of rat Cognitive Aptitude Test results

The result shows that compare with Sham-operated control group, the chronic cerebral ischemia group reaches association's total degree that standard shocks by electricity significantly increases (P＜0.01); Compare with the chronic cerebral ischemia group, red seven groups reach association's total degree that standard shocks by electricity and significantly reduce (P＜0.01) with American ginseng DANQI PIAN group, and red seven groups and ischemic protection group indifference.The result sees table 1

Four groups of rat Cognitive Aptitude Tests of table 1 result

Annotate: compare with Sham-operated control group, ^△P＜0.01, with the comparison of chronic cerebral ischemia group, * p＜0.05, * * p＜0.01, with red seven groups of comparisons, ¹P＜0.05

2.2 four groups of rat hippocampus CAI zone GFAP positive cell situation

The result shows, compares with sham operated rats, and the GFAP positive cell number significantly increases (P＜0.01) under average each high power lens of chronic cerebral ischemia group; Compare with the chronic cerebral ischemia group, the GFAP positive cell number significantly reduces (P＜0.01) under average each high power lens of American ginseng DANQI PIAN group, and red seven groups and ischemic protection group indifference.See table 2.

Four groups of rat hippocampus CAI zones of table 2 GFAP positive cell is

relatively

Three, discuss

In this experiment, compare with sham operated rats, the chronic cerebral ischemia group reaches association's total degree that standard shocks by electricity significantly to be increased; Compare with the chronic cerebral ischemia group, American ginseng DANQI PIAN group reaches association's total degree that standard shocks by electricity and significantly reduces, and explain that chronic cerebral ischemia group learning and memory in rats ability significantly descends, and American ginseng DANQI PIAN group learning and memory in rats ability is improved significantly.The GFAP positive cell number significantly increases under average each high power lens of chronic cerebral ischemia group; The GFAP positive cell number significantly reduces under average each high power lens of American ginseng DANQI PIAN group; Explain that a large amount of hyperplasia appear in chronic cerebral ischemia group Rat Astroglia, and ischemic protection group hyperplasia is lighter.Astroglia is being brought into play important physiology and pathological effect in central nervous system; This type of cell produces kickback to various nervous lesions; GFAP is the astroglia specificity marker protein, and its overexpression can be thought the hyperplasia sign under the pathologic condition.

This experiment shows that the American ginseng DANQI PIAN can alleviate neuronic damage and forfeiture; Reduce the astroglia hyperplasia that the chronic cerebral anoxic is caused, alleviate brain tissue damage.In sum, the American ginseng DANQI PIAN reaches the effect of protection brain tissue through alleviating the nervous lesion that chronic cerebral ischemia causes.

Eight, gained American ginseng DANQI PIAN auxiliary improving memory function human feeding trial of the present invention

One, materials and methods

1, sample

The main constituent of American ginseng DANQI PIAN: American Ginseng accounts for 26%, the red sage root 58%, pseudo-ginseng 16%.

Specification: 0.40g/ sheet, 24/box.Recommending dose is every day 3 times, each 2, uses warm water delivery service.

2, test method

Human feeding trial rules according to auxiliary improving memory function in " health food check and assessment technique standard " (version in 2003) are carried out; Adopt the Clinical Memory scale to press the memory quotient that test request is measured the experimenter, main examiner person is through special training and obtain certificate.

3, test scale

Adopt " the Clinical Memory scale " of Institute of Developed Organisms, Academia Sinica's revision in 1996.

4, select experimenter's principle

(1) from the colony relatively more concentrated, that the each side influence factor is roughly the same, selects the volunteer; (2) schooling basically identical; (3) belong to age-grade group,, then tackle scale score and proofread and correct as not at age-grade group; (4) do not accept similar test; (5) article relevant with being tried function are taken in eliminating in a short time, have influence on the judgement to the result.

5, test-meal object and grouping

Concentrate and select study subject, implement informed consent.Take before the given the test agent experimenter carried out memory quotient test first time after, (MQ) is divided into test-meal group and control group at random with the experimenter by memory quotient, considers educational level, age, sex etc. as far as possible, carries out harmony and checks.Number 107 people are selected in test-meal, and that accomplish full test-meal process is 104 people, wherein test-meal group 53 people, and control group 51 people lose visit rate 2.80%.

6, test-meal method

Control group is taken placebo, and the test-meal group is taken the American ginseng DANQI PIAN, takes each 2 every day 3 times.Took continuously 1 month.Abide by the double blinding principle in the test-meal.

7, observation index

7.1 safety indexes

7.1.1 ordinary circumstance: spirit, sleep, diet, the interrogation of stool and urine situation.

7.1.2 routine inspection: blood, urine, just routine inspection.

7.1.3 biochemical indicator is measured: liver, renal function, like total serum protein (TP), albumin (ALB), ALT (ALT), the amino transaminase (ALT) of aspartic acid, urea nitrogen (BUN), creatinine (Cr) etc.

7.1.4 Chest X-rays, electrocardiogram, Abdominal B type ultrasonography inspection (inspection is 1 time before only being tried).

7.2 effect index

Adopt the Clinical Memory scale, with the original branch of each subtest after the test, look into scale score, each subtest scale score addition gets total scale score, looks into memory quotient (MQ) with total scale score.(1) points to the memory scale score; (2) associative learning scale score; (3) the random shape amount of re-recognizing branch; (4) the image scale score of freeing recall; (5) scale score is recalled in the contact of portrait characteristics; (6) memory quotient.

8, the result judges

Under the prerequisite that two groups of memory quotient are balanced before test; The memory quotient of test-meal group is higher than control group after the test-meal; And difference has statistical significance; Memory quotient after the test-meal of the group of test-meal simultaneously is higher than the memory quotient before its test-meal, and difference has statistical significance can judge that this given the test agent has the effect of auxiliary improving memory function.

9, statistical procedures

Test data is a measurement data, and two groups of each subtest scale scores and memory quotient are analyzed with the t check.Own control is used paired t-test; Relatively with two sample mean volume t check, the latter need carry out homogeneity test of variance between group, and the data of Non-Gaussian Distribution or heterogeneity of variance are carried out suitable variable conversion; Wait to satisfy normal state or variance neat after, carry out the t check with the data that transform; If conversion data still can not satisfy the neat requirement of normal state or variance, use t check or rank test instead, but the too big data of the coefficient of variation is used rank test.There is statistical significance P＜0.05 for difference.

Two, result

1, the American ginseng DANQI PIAN is to the influence of experimenter's ordinary circumstance and safety indexes

Test-meal American ginseng DANQI PIAN experimenter ordinary circumstance is good, and the sleep of test-meal group, diet situation are significantly improved after the test-meal, and control group sleep, diet, stool and urine situation are significantly improved, and not seeing has harmful effect to ordinary circumstance.All in normal range (NR), urine, stool routine examination are not all seen obviously unusual for each item hematology and liver, renal function index before and after test-meal group, the control group test-meal.Inspection Chest X-rays, electrocardiogram, Abdominal B type ultrasonography are not all seen obviously unusual before being tried.Show that test-meal American ginseng DANQI PIAN is not to human body blood, urine, just routine and liver, renal function etc. produce harmful effect.

2, test-meal group and control group are relatively harmonious

All information person 104 people that tried, wherein test-meal group 53 examples, control group 51 examples can be found out by table 1, memory quotient, schooling, age, the equal not statistically significant of sex composition aspect difference before test-meal group and control group are tried show that two groups of equilibriums are comparable.

The preceding two groups of experimenters' of table 1 test ordinary circumstance

3, the result of each item subtest scale score before and after the test-meal

Table 2 is that each subtest scale score compares before two groups of test-meals.The result shows: test-meal group and control group compare before the test-meal, the equal not statistically significant of each item subtest scale score difference; Test-meal group sensing memory, image are freed recall after the test-meal, random shape is re-recognized, image free, recall five subtest scale scores of portrait characteristics contact memory all is significantly higher than control group, and associative learning and control group relatively do not have the conspicuousness difference; Before each item index is higher than test-meal after the contrast test-meal, possibly the 2nd performance of the test increased because of being familiar with content measurement and method.

Preceding two groups of each the subtest scale score situation of table 2 test-meal

Two groups of each subtest scale score situation

after table 3 test-meal

Annotate: compare * p＜0.05 with control group

4, total scale proportion by subtraction is remembered for two groups in food test-meal front and back

Visible by table 4: total scale score of test-meal group and control group difference not statistically significant before the test-meal; The test-meal group is remembered total scale score and is significantly higher than control group after the test-meal; Before memory total amount table score value is significantly higher than test-meal after the test-meal of test-meal group, on average raise 8.5 fens, and be significantly higher than control group.The total scale of memory distributes before and after the test-meal of test-meal group has statistical significance to comparing difference, and control group difference not statistically significant.

Memory total scale score situation

before and after the table 4 liang group test-meal

Annotate: P ' is test-meal front and back self paired comparisons

5, food is to the influence of experimenter's memory quotient

Visible by table 5: test-meal group memory quotient and control group compare before the test-meal, the difference not statistically significant; Test-meal group memory quotient is significantly higher than control group after the test-meal; Before memory quotient is significantly higher than test-meal after the test-meal of test-meal group, on average rose 9.5 fens, be significantly higher than control group; Memory quotient paired comparisons difference has statistical significance before and after the test-meal of test-meal group, and control group difference not statistically significant.

Improve memory quotient branch situation

before and after the table 5 liang group test-meal

Annotate: P ' is test-meal front and back self paired comparisons.

Three, discuss

Examination trencherman ordinary circumstance before and after the test-meal American ginseng DANQI PIAN, blood, urine, just routine and liver, the inspection of kidney biochemical function are not all seen obviously unusually, show that test-meal American ginseng DANQI PIAN do not see harmful effect to human body.53 routine experimenter's test-meal American ginseng DANQI PIANs are after 1 month, and test-meal group memory quotient is significantly higher than before memory quotient is significantly higher than test-meal after the test-meal of control group test-meal simultaneously group.The result shows that American ginseng DANQI PIAN of the present invention has auxiliary improving memory function to human body.

Nine gained American ginseng DANQI PIAN auxiliary improvement sleep function human feeding trials of the present invention

One, clinical research

1, clinical data

1.1 Western medicine diagnose standard

Western medicine diagnose is with reference to the diagnostic criteria of " Chinese phrenoblabia classification and diagnostic criteria " relevant insomnia.Chinese medical discrimination is with reference to the diagnostic criteria in " traditional Chinese medical science disease diagnosis criterion of therapeutical effect ", " the new Chinese medicine clinical research guideline ".

1.2 include in and exclusion standard

Include standard in: meet above-mentioned diagnostic criteria, show as be difficult to fall asleep, be prone to wake up, sleep is dark, dreaminess, early awakening, the back of waking up be difficult for again sleeping, daytime is sleepy, absent minded, failure of memory, vexed, palpitaition, breathe hard, spontaneous perspiration; Sleep incubation period＞30 minute occur weekly more than 3 times; 17～75 years old age.Exclusion standard: physical disease, severe hepatic, renal insufficiency; Lose compensatory pulmonary insufficiency or merge other each serious disease persons of system; Cat fever; Gestation or women breast-feeding their children etc.

1.3 case is selected

In the period of the 2010-2011, outpatient service is diagnosed as insomniac's 89 examples, and drawing lots by the simple randomization principle is divided into three groups, and 31 examples are organized in treatment, male 15 examples, women 16 examples; Age 16～～67 years old, average (51.23 ± 2.41) year.Control group 30 examples, male 13 examples, women 17 examples; 19～65 years old age, average (52.56 ± 2.72) year.Negative control group 28 examples, male 11 examples, women 17 examples; Age 19～～63 years old, average (50.56 ± 2.36) year.Difference not statistically significants (P＞0.05) such as three groups of sexes, age, the course of disease have comparativity.

2, method

2.1 methods of treatment

Negative control group is taken placebo, and control group is taken Anshen Bunao mixture+placebo tablet, and the test-meal group is taken American ginseng DANQI PIAN+placebo oral liquid, and tablet is taken 3 times every day, each 2.Oral liquid every day 2 times, each 1 bottle.Took continuously 1 month.Abide by the double blinding principle in the test-meal.

2.2 observation index and method

All according to the scoring of SPIEGEL sleep scale, SPIEGEL sleep scale scoring >=12 is divided into no sleep-disorder before and after two groups of patient's test-meals.(seeing table 1)

The scoring of table 1SPIEGEL sleep scale

2.3 curative effect determinate standard

Carry out therapeutic evaluation according to State Administration of Traditional Chinese Medicine's " traditional Chinese medical science disease diagnosis criterion of therapeutical effect ".Clinical recovery: sleep is normal, and more than the length of one's sleep 6h, simultaneous phenomenon disappears; Effectively: total sleep time prolongs more than the 2h, but not enough 6h, simultaneous phenomenon improves; Invalid: before sleeping as taking medicine, or prolong not enough 2h the length of one's sleep, symptom does not have improvement.

Two, result

3.1 two groups of recent general curative effects of patient relatively

The result can be known by table 2: treatment group and control group comparing difference do not have has statistical significance (P＞0.05).

Table 2 clinical efficacy relatively

3.2 SPIEGEL sleep scale integration relatively before and after two groups of patient treatments

The result is as shown in table 3: self treating the front and back comparing difference for two groups all has statistical significance (P＜0.01), and the improvement degree of treatment group is superior to control group (P＜0.05), and relatively there were significant differences (P＜0.01) with negative control group.

The variation

of SPIEGEL sleep scale integration before and after the table 3 liang group treatment

Annotate: compare with control group, ^△P＜0.05, relatively preceding with treatment, compare with negative control group * p＜0.01 ¹P＜0.01.

Three, discuss

This research is relatively treated quite with Anshen Bunao mixture after showing treatment; But the improvement degree is obvious than Anshen Bunao mixture; Show that American ginseng DANQI PIAN of the present invention can improve sleep and therapeutic process safety, reliable effectively; And during treating, do not find bad reaction, bad reactions such as dizziness, dry, sleepy, liver damage occur.Take no dependence for a long time, do not have withrawal symptom after the drug withdrawal, determined curative effect, bad reaction is few.

The tenth part gained American ginseng of the present invention DANQI PIAN is treated stable anginal clinical observation

One, clinical research

1, clinical data

1.1 Western medicine diagnose standard

The MethodsThe cases enrolled standard: 1. fatigue, diet, cold or be prone to bring out when excited, the unexpected pain of chest, often be positioned at breastbone after, can be radiated to left side shoulder, the left upper extremity inboard reaches the third finger and little finger of toe.Pain character can be squeezing property, vexed bloated property, and girdle sensation or constriction also can be burn feeling.General pain time is lighter, continues 3～5 minutes, seldom above 20 minutes.But like this outbreak a couple of days 1 time, but also several weeks 1 time.Common increased heart rate when 2. showing effect, blood pressure raises, and pale complexion is in a cold sweat etc.3. can have during pain nonspecific SL section and T ripple unusual between Electrocardioscopy, about half patient is in normal range (NR); The ST section person of forcing down who occurs temporary myocardial ischemia during angina pectoris attacks can do dynamic Electrocardioscopy, therefrom finds the change of ST-T and relation, duration and the excursion of various arrhythmia cordis and symptom.

Get rid of the case standard: 1. acute myocardial infarction AMI: how indeterminate the pain inducement is, and pain is more violent, and longer duration can reach several hours or longer.Have a rest or contain the clothes nitroglycerine tablets and can not alleviate, the patient often is in a cold sweat, dying sense.Gastrointestinal symptoms such as nauseating, vomiting, epigastric pain can be arranged, and electrocardiogram has specificity to change.2. cardiac neurosis: pectoralgia is transience, be mostly tired back rather than the time contain obey that nitroglycerine tablets are invalid, pain, insomnia etc.Other no specificity or nonspecific T wave changes.3. intercostal neuralgia: limit office is at intercostal, with local tenderness.Normally be acupuncture appearance, the occurred fash that has, Electrocardioscopy is no abnormal.

1.2 case is selected

Special disease kind patient 48 examples are accepted in August, 2009～20010 a year outpatient service in December for medical treatment, male 40 examples, women 8 examples, 48～73 years old year, average 63 years old; The course of disease 3～18 years.

2, method

2.1 methods of treatment

Control group gives the compound danshen dripping pills of sky, oral Tianjin Shi Li drugmaker development, and is each 10,3 times/d, oral.The treatment group is taken DANQI PIAN and is taken 3 times every day, each 2.The all identical medications of measure such as other aspirin, metoprolol, ACEI, lipid-lowering statins and control blood sugar.

2.2 observation index and method

2.2.1 record angina pectoris attacks situation during the observation index medication, adverse drug reaction is done secondary routine electrocardiogram (ECG) inspection weekly, traces electrocardiogram during angina pectoris attacks at once, and contrast tranquillization ECG changes before and after the treatment.

2.2 efficacy determination

(1) clinical efficacy criterion, produce effects: equal tired degree does not cause that angina pectoris or angina pectoris attacks reduce more than 80%; Effectively: the angina pectoris attacks number of times reduces or reduces number of times and surpasses the preceding sum 50%～80% of treatment; Invalid: the angina pectoris attacks number of times reduce less than 50% or symptom do not have improvement.(2) the ECG efficacy determination is accurate: it is produce effects that ECG recovers normally, and it uprightly is effectively that the ST section is improved >=50% or 1/1 ripple, and produce effects is with effectively both add up to improvement, and it is invalid not reaching above-mentioned standard.

2.3 statistical procedures

Adopt t check or X ²Check, there is statistical significance P＜0.05 for difference.

Two, result

2.1 the angina pectoris clinical efficacy relatively

The result can be known by table 1: treatment group and control group comparing difference do not have has statistical significance (P＞0.05).

2.2 the angina pectoris attacks situation relatively

See table 2.Significantly reduce with later anginal seizure frequency of American ginseng DANQI PIAN treatment and duration.And the duration that shortens has been compared significant difference with the duration that compound danshen dripping pills shortens, and explains that the American ginseng DANQI PIAN can well reduce stable anginal outbreak.

Table 2 liang group angina pectoris attacks situation is

relatively

Annotate: compare with control group, ^△P＜0.05, relatively preceding with treatment, * p＜0.05, * * p＜0.01

Three, discuss

This research is relatively treated quite with compound danshen dripping pills after showing treatment; But it is obvious than compound danshen dripping pills to shorten the angina pectoris attacks time; Show that American ginseng DANQI PIAN of the present invention can reduce anginal outbreak effectively; And during treating, do not find bad reaction can well improve patient's quality of life.

Embodiment two

The health food of present embodiment is processed by following component: American Ginseng 155 grams, the red sage root 350 grams, pseudo-ginseng 100 grams, process 1000 altogether.

In other embodiments, the prescription of health food also can be:

Sequence number	American Ginseng (weight portion)	The red sage root (weight portion)	Pseudo-ginseng (weight portion)
				1	1	9	2
2	6	8	1
				3	6	16	5
4	3	12	4
				5	5	14	2
6	4	10	3

Method for making: adopting concentration is that the alcohol heating reflux red rooted salvia of 90%-98% therefrom extracted the red sage root in 1.5 hours, and it is 50% alcohol heating reflux 1.5 hours that the dregs of a decoction add concentration, and residue water extracts, and dregs of a decoction boiling 2 hours obtains red sage root concentrate; Pseudo-ginseng, ginseng powder are broken into fine powder (or ultra micro powder), process the Chinese medicine composition of fine-powder with above-mentioned red sage root concentrate, add appropriate amount of auxiliary materials, mixing, and drying, compress tablet coating is processed lozenge or encapsulated, promptly gets.

In other embodiments, the time of red rooted salvia reflux can be 1～2h, can select 1h or 2h or 1.2h or 1.5h etc.; The time of dregs of a decoction reflux is 1～2h, and the time of dregs of a decoction boiling can be 1.5～2.5h, and decocting liquid filters, and filtrating is concentrated into an amount of.Concentration of alcohol used during dregs of a decoction reflux can be 45%～95%, as selecting 45% or 60% or 80% or 90% or 95%.

More than the present invention has been carried out foreign thin introduction, use concrete example in the literary composition principle of the present invention and embodiment set forth, the explanation of above embodiment just is used for helping to understand method of the present invention and core concept thereof.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.

Claims

1. a health food is characterized in that, is processed by the raw material of following weight portion: 1～6 part of American Ginseng, 8～16 parts of the reds sage root, 1～5 part of pseudo-ginseng.

2. health food according to claim 1 is characterized in that, is processed by the raw material of following weight portion: 2～5 parts of American Ginsengs, 9～15 parts of the reds sage root, 2～4 parts of pseudo-ginseng.

3. health food according to claim 1 is characterized in that, is processed by the raw material of following weight portion: 2 parts of American Ginsengs, 14 parts of the reds sage root, 4 parts of pseudo-ginseng.

4. the preparation method of any described health food of claim 1～3 is characterized in that, comprises the steps:

5. preparation method according to claim 4 is characterized in that, in the step 1), adopts CO ₂The process of the supercritical fluid extraction red sage root is:

6. preparation method according to claim 4 is characterized in that: in the step 1), adopt the process of the alcohol extract red sage root to be:

1a ') ethanol of adding concentration 90%-98% in red rooted salvia, reflux 1～2h filters extract, and filtrate recycling ethanol also is concentrated into an amount of, subsequent use;

1c ') dregs of a decoction boiling 1.5h～2.5h, decocting liquid filters, and filtrating is concentrated into an amount of.

7. the application of health food in the diabetes complicated disease drug of preparation treatment that make of each described health food of claim 1～3 or each preparation method of claim 4～6.

8. the application of health food in preparation Prevention and Treatment of Osteoporosis medicine that make of each described health food of claim 1～3 or each preparation method of claim 4～6.

9. the application of health food in preparation treatment nephrotic syndrome medicine that make of each described health food of claim 1～3 or each preparation method of claim 4～6.

10. the application of health food in the nervous lesion disease drug that preparation treatment chronic cerebral ischemia causes that make of each described health food of claim 1～3 or each preparation method of claim 4～6.

11. the application of the health food that each described health food of claim 1～3 or each preparation method of claim 4～6 make in preparation old failure of memory of control or senile dementia disease drug.

12. the application of the health food that each described health food of claim 1～3 or each preparation method of claim 4～6 make in preparation treatment cardiovascular and cerebrovascular diseases medicament.

13. the health food that each described health food of claim 1～3 or each preparation method of claim 4～6 make improves the application in the sleep type medicine in preparation.

14. the health food that each described health food of claim 1～3 or each preparation method of claim 4～6 make improves the application in the old memory medicine in preparation.

15. the health food that each described health food of claim 1～3 or each preparation method of claim 4～6 make improves the application aspect the body anoxia endurance medicine in preparation.