CN112426447A

CN112426447A - American ginseng, salvia miltiorrhiza and pseudo-ginseng pill and preparation method and application thereof

Info

Publication number: CN112426447A
Application number: CN202011157448.6A
Authority: CN
Inventors: 孙舒洁; 孙润广; 张静
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-10-26
Filing date: 2020-10-26
Publication date: 2021-03-02

Abstract

The invention belongs to the technical field of traditional Chinese medicine preparations, and relates to an American ginseng, salvia miltiorrhiza and pseudo-ginseng pill and a preparation method and application thereof, wherein the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill comprises American ginseng, salvia miltiorrhiza, pseudo-ginseng, stiff silkworm and leech; the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 0.8-1.2: 0.2-0.5: 0.2-0.6, and the preparation method comprises the following steps: 1) taking five traditional Chinese medicines of American ginseng, salvia miltiorrhiza, pseudo-ginseng, stiff silkworm and leech; respectively grinding into powder; mixing, concocting with Mel, and making into pill. The traditional Chinese medicine is used for conditioning, so that the fibrinolytic performance of thrombus is improved, and the thrombus is effectively inhibited; can improve the hypoxia tolerance of human body; also has the effects of calming the nerves and calming the nerves, eliminating fatigue, enhancing memory and improving sleep quality, and has mild medicinal effect and small side effect.

Description

American ginseng, salvia miltiorrhiza and pseudo-ginseng pill and preparation method and application thereof

Technical Field

The invention belongs to the technical field of traditional Chinese medicine preparations, and relates to an American ginseng, salvia miltiorrhiza and pseudo-ginseng pill as well as a preparation method and application thereof.

Background

Arteriosclerosis is a vascular disease which appears with age, and the rule is that the arteriosclerosis usually occurs in teenagers and aggravates and attacks the middle-aged and elderly people. The main causes of arteriosclerosis are hypertension, hyperlipidemia and smoking. Other conditions such as obesity, diabetes, hypokinesia, stress, advanced age, family history, and irritable bowel syndrome can cause arteriosclerosis. From a nutritional perspective, the primary cause of arteriosclerosis is a cholesterol and lipid diet; the ingestion of a large amount of greasy food and cholesterol-rich food, cholesterol cannot dissolve in the body, resulting in thickening of the vessel wall and eventual thrombosis. When thrombus occurs, the blood vessels of a human body are insufficient in blood supply due to the blockage of the blood vessels, so that oxygen deficiency is caused, and symptoms such as shock and the like can occur.

Currently, for patients with arteriosclerosis, vasodilators, blood lipid regulators, anti-platelet adhesion and aggregation inhibitors, thrombolytic agents and anticoagulant agents are used. Generally, the medicines are medicines for resisting platelet adhesion and aggregation, thrombolytic medicines or anticoagulant medicines, which have single effect and cannot well deal with other symptoms (palpitation, chest pain, chest distress, headache, dizziness, limb numbness, limb weakness, claudication, visual deterioration, memory deterioration, insomnia, dreaminess and the like) accompanied by arteriosclerosis although the medicines can play a role in preventing thrombosis; the fibrinolytic effect of thrombus is not ideal; and the western medicines are expensive and have large side effects, which can treat the symptoms and not the root causes.

Disclosure of Invention

The invention aims to disclose American ginseng, salvia miltiorrhiza and pseudo-ginseng pills and a preparation method and application thereof, which improve the fibrinolytic performance of thrombus and effectively inhibit the formation of thrombus by conditioning traditional Chinese medicines; can improve the hypoxia tolerance of human body; also has the effects of calming the nerves and calming the nerves, eliminating fatigue, enhancing memory and improving sleep quality, and has mild medicinal effect and no side effect.

In order to achieve the purpose, the invention adopts the technical scheme that:

a pill, XIYANGSHENDANQI pill, comprises radix Panacis Quinquefolii, Saviae Miltiorrhizae radix, Notoginseng radix, Bombyx Batryticatus and Hirudo; the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 0.8-1.2: 0.2-0.5: 0.2 to 0.6.

Further, the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 1: 0.2-0.5: 0.2 to 0.6.

Further, the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 1: 0.3: 0.2 to 0.6.

Further, the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 1: 0.3: 0.4.

a preparation method of American ginseng, salvia miltiorrhiza and pseudo-ginseng pills comprises the following steps:

1) taking five traditional Chinese medicines of American ginseng, salvia miltiorrhiza, pseudo-ginseng, stiff silkworm and leech;

2) grinding the five traditional Chinese medicines in the step 1) into powder with the granularity of 50-100 mu m respectively;

3) mixing the five powdered traditional Chinese medicines in the step 2) according to the mass ratio of any one of claims 1-4, blending with honey, and making into pills.

A pill, XIYANGSHENDANQI pill, is administered at a dose of 3-6g each time and 2 times per day.

An application of pill containing radix Panacis Quinquefolii, Saviae Miltiorrhizae radix and Notoginseng radix in improving thrombofibrinolysis is provided.

An application of pill containing radix Panacis Quinquefolii, Saviae Miltiorrhizae radix and Notoginseng radix in improving anoxia resistance of human body is provided.

The invention has the beneficial effects that:

1. the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the invention comprises American ginseng, salvia miltiorrhiza, pseudo-ginseng, stiff silkworm and leech; the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 0.8-1.2: 0.2-0.5: 0.2 to 0.6. Through the synergistic effect of the traditional Chinese medicines of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech, the medicine effect is mild, the fibrinolytic performance of thrombus is improved, and the thrombus formation is effectively inhibited; can improve the hypoxia tolerance of human body; also has effects of tranquilizing mind, calming qi, relieving fatigue, improving memory, and improving sleep quality, and has no side effect.

2. The invention provides American ginseng, salvia miltiorrhiza and pseudo-ginseng pills, and during preparation, the traditional Chinese medicines are ground into powder; mixing, adding Mel, and making into pill; the preparation method is simple, and the honey can relieve the medicinal taste of the traditional Chinese medicine and is convenient for oral administration.

Detailed Description

The present invention will now be described in detail with reference to examples.

The American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the invention comprises American ginseng, salvia miltiorrhiza, pseudo-ginseng, stiff silkworm and leech.

American ginseng: american ginseng (scientific name: Panax quinquefolius) is a perennial herb belonging to the genus Panax of the family Araliaceae, and is also known as American ginseng, American ginseng or American ginseng; cool in nature, sweet and slightly bitter in taste; it has effects of invigorating lung, lowering fire, nourishing stomach, and promoting fluid production; mainly used for deficiency of vital energy and yin, internal heat, cough, asthma, phlegm and blood, asthenic fever, vexation, fatigue, diabetes, dry mouth and throat, lung yin benefiting, deficiency fire clearing, body fluid production promoting and thirst quenching; it is indicated for chronic cough due to lung deficiency, blood loss, dry throat, thirst, deficiency heat, restlessness and fatigue. The American ginseng has the functions of strengthening central nerves, promoting protein synthesis required by a human body and improving the immunity of the human body; can also inhibit the growth of cancer cells and has certain anticancer effect; can effectively regulate the blood pressure of a human body and promote the blood vitality; promote metabolism of sugar and fat in human body, and has certain adjuvant therapeutic effect on diabetes.

Red sage root: is dried root and rhizome of Salvia Miltiorrhiza bge. Bitter and slightly cold in taste; heart and liver meridian entered; has the effects of promoting blood circulation, removing blood stasis, stimulating the menstrual flow, relieving pain, clearing away the heart-fire, relieving restlessness, cooling blood and eliminating carbuncle. Can be used for treating thoracic obstruction, heart pain, abdominal pain, hypochondriac pain, abdominal mass, pain due to pyretic arthralgia, vexation, insomnia, menoxenia, dysmenorrhea, amenorrhea, and pyocutaneous disease with swelling and pain. The Saviae Miltiorrhizae radix contains tanshinone, protocatechuic aldehyde, protocatechuic acid, tanshinol, vitamin E, etc. Tanshinone is the most important functional component. The radix salviae miltiorrhizae can enhance myocardial contraction force, thereby improving the cardiac function; can dilate coronary artery, increase coronary blood flow, improve myocardial ischemia, infarction and heart function, regulate heart rhythm, dilate peripheral blood vessel, and improve microcirculation; the recovery period of red blood cells and hemoglobin is shortened, the reticulocytes are increased, the tissue repair can be promoted, and the fracture healing can be accelerated; the salvia miltiorrhiza contains bioactive substances, so that the activity of body cells can be improved, and redundant free radicals in a body can be eliminated so as to prevent the free radicals from being oxidized, and the effect of delaying senescence is achieved.

Pseudo-ginseng: the name of the traditional Chinese medicine. Is dried root of Panax notoginseng (Burk.) F.H.Chen of Araliaceae. Sweet, slightly bitter and warm; it enters liver and stomach meridians. Has the functions of eliminating blood stasis, stopping bleeding, eliminating swelling and relieving pain. Has effects of removing blood stasis, stopping bleeding, promoting blood circulation, and relieving pain. It can be used for treating hemorrhage, traumatic injury, and swelling and pain due to blood stasis. The notoginseng powder is a root and stem product of plant notoginseng (scientific name: Panax pseudo-ginseng), has warm nature, sweet and slightly bitter taste, and enters liver, stomach and large intestine channels. The Notoginseng radix powder has effects of dilating blood vessel, lowering blood pressure, stopping bleeding, promoting blood circulation, improving sleep, resisting aging, resisting cancer, resisting inflammation, protecting liver, and relieving pain. The pseudo-ginseng powder has the effects of promoting blood circulation to remove blood stasis, and relieving swelling and pain. Has good myocardial protection effect; has antiarrhythmic effect, and is effective in reducing blood lipid, preventing atherosclerosis, and lowering blood pressure.

Stiff silkworm: a dry larva lethal to Beauveria bassiana (Bals.) Vuillant infected (or artificially inoculated) with larva of 4-5 th instar of Bombyx mori Linnaeus, an insect of Bombycidae. Neutral in nature, has the effects of calming endogenous wind, relieving spasm, eliminating phlegm, resolving masses, and the like, can be used for treating symptoms such as wind-heat headache, skin pruritus, facial paralysis, and the like, dispelling wind, arresting convulsion, eliminating phlegm, resolving masses; bombyx Batryticatus can be used for treating convulsion, sore throat, skin pruritus, submandibular lymphadenitis, and facial paralysis. Has effects in treating migraine and temple pain. Bombyx Batryticatus can be used for treating phlegm eliminating, stagnation resolving, apoplexy, aphonia, convulsion, oral cavity, mouth and eye deviation, laryngopharynx swelling and pain, laryngopathy, headache, enteropathogenic wind, hematochezia, tetany, and obnubilation.

Leech: bitter in taste, moderate in nature. The efficacy is as follows: promoting blood circulation, removing blood stasis, dredging channels and removing food retention. It can be used for treating blood stagnation, amenorrhea, traumatic blood stasis, and abdominal mass. Mainly contains hirudin, heparin and other components; has anticoagulant effect, can dissociate thrombin and platelet, and can remarkably reduce the formation of glomerular microthrombus, reduce the consumption of blood coagulation factors and platelets and prevent acute renal failure by hirudin intravenous injection; has obvious effect of resisting myocardial ischemia and arrhythmia; reducing blood lipid and resisting inflammation.

The five traditional Chinese medicines have synergistic effect, and the fibrinolytic performance of thrombus is improved and the thrombus formation is effectively inhibited through the conditioning of the traditional Chinese medicines; improving the hypoxia tolerance of the human body; also has the effects of calming the nerves and calming the nerves, eliminating fatigue, enhancing memory and improving sleep, and has mild medicinal effect and small side effect.

Examples 1 to 10

The mass ratio of the American ginseng, salvia miltiorrhiza and panax notoginseng pills in the embodiment is shown in table 1.

Example 1-example 10 mass ratio of American ginseng, salvia miltiorrhiza and panax notoginseng pills

Example 11

The preparation method of the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the embodiment comprises the following steps:

3) mixing the five powdered traditional Chinese medicines in the step 2) according to the mass ratio provided in the embodiments 1-10, blending with honey, and making into pills.

Further, in order to illustrate the use effect of the pill containing American ginseng, red sage root and pseudo-ginseng provided by the invention, the following tests are carried out.

Test 1

The fibrinolytic activity of the American ginseng, salvia miltiorrhiza and pseudo-ginseng pills provided by the invention is demonstrated through a white mouse comparison test.

Comparison group: blank group

Test groups: the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the embodiment 7 of the invention

The test method comprises the following steps: feeding 50 mice with high-fat feed every day to obtain a white mouse model with moderate thrombus symptom, selecting 30 mice from the mice, weighing 20 +/-2 g, randomly dividing into 2 groups, and each group comprises 15 mice, wherein: a first group: 15 mice were injected with 3ml of sterile water every morning and evening; second group: the American ginseng, red sage root and pseudo-ginseng pills (the concentration is 3g of American ginseng, red sage root and pseudo-ginseng pills/10 ml of sterile water) provided by a test group are injected into 15 mice every morning and evening.

Blood samples were drawn in sequence every 7 days, and plasma plasminogen activity PLG in plasma was measured by calculation using chromogenic substrate method: the A content, and then the 15 results per group were averaged to obtain an average value, and the results are shown in Table 1.

	Control group	Test group
			Day 0	1.84	1.86
7 days	1.73	1.65
			14 days	1.78	1.4
21 days	1.82	1.2
			28 days	1.9	1.0

Plasma plasminogen active PLG: normal values for a were 0.75-1.4, as can be seen from the data, plasma plasminogen active PLG: an increased A content indicates a decreased fibrinolytic activity in blood, indicating a prothrombotic state and thrombotic disease.

As can be seen from table 1, the use of the panax quinquefolium, salviae miltiorrhizae and panax notoginseng pills of the experimental group provided by the present invention results in a plasma plasminogen activity PLG: a content decreases, indicating an increased fibrinolytic activity on blood, leading to a decrease in plasma plasminogen active PLG: the A is restored to a normal level, which shows that the American ginseng, salvia miltiorrhiza and panax notoginseng pill provided by the invention has good fibrinolytic activity and is beneficial to inhibiting thrombus.

Test 2

The control experiment of mice shows that the American ginseng, salvia miltiorrhiza and pseudo-ginseng pills provided by the invention can inhibit thrombus.

Comparison group: blank group

Test groups: the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the embodiment 3 of the invention

The test method comprises the following steps: feeding 50 adult mice with high-fat feed every day to obtain a white mouse model with moderate thrombus symptom, and randomly dividing 20 adult mice with weight of 20 +/-2 g into 2 groups of 10 mice each, wherein: a first group: 10 mice were fed 2g of sterile water every morning and evening; second group: 10 mice were fed with 2g of American ginseng, salvia miltiorrhiza and pseudo-ginseng pills provided by the test group every morning and evening.

Blood samples were sequentially taken every 7 days, the platelet aggregation rate of the blood samples was measured using an optical platelet aggregation meter, and then 10 results per group were averaged to obtain an average value, and the results are shown in table 2.

	Control group	Test group
			Day 0	90％	91％
7 days	92％	88％
			14 days	88％	80％
21 days	89％	73％
			28 days	92％	65％

Generally, the normal value of the platelet aggregation rate is 50-84%, and when the platelet aggregation rate is increased, the characteristic of adhesion between platelets is increased, and the platelet aggregation rate is often observed in a prothrombotic state or a thrombotic disease.

From the results shown in table 2, the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the invention can reduce the platelet aggregation rate and maintain the platelet aggregation rate in a normal range, which indicates that the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill can play a good role in inhibiting thrombus.

Test 3: hypoxia tolerance test

The principle of the normal-pressure hypoxia-resistant test is as follows: hypoxia is a stressor to the matrix, affects various metabolisms of the matrix, particularly affects the oxidation function of the matrix, and finally leads to the death of the matrix due to insufficient hypoxia function of the major organs of the matrix, such as heart, brain and the like.

Test animals, adult mice of single sex, 18-22g, 10 animals per group, four groups.

Materials: 205ml ground bottle, stopwatch, vaseline, soda lime

And (3) experimental setting: 1 group of blank and the other three groups of pills are fed with the American ginseng, the red sage root and the pseudo-ginseng pills provided by the embodiment 8 of the invention every morning and evening, and the feeding dosage of the three groups of pills is 1 time, 3 times and 10 times of that of a human body respectively. The test samples were given for 30 days.

Each dosage group is administrated by stomach filling, the blank group is administrated by sterile water, after the last stomach filling for 1h, each group of animals are respectively put into a 250ml ground bottle filled with soda lime, one bottle is sealed by vaseline, the bottle is tightly covered to ensure that the bottle is airtight, the time is immediately timed, the stopping of breathing is taken as an index, the death time of the mice due to oxygen deficiency is observed, and the average value of each group of results is calculated; see table 3 for results.

TABLE 3 comparison of hypoxic death time at different doses

	Blank group	10 times the dosage	3 times the dosage	1 time dosage
					Time to death due to hypoxia	3.6min	4.8min	4.6min	4.5min

From the result, the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the invention can effectively prolong the hypoxia tolerance time.

Test 4

Randomly selecting 6 patients suffering from arteriosclerosis, collecting original blood samples to analyze arterial thrombosis detection indexes, wherein the 1 st patient takes the medicine provided in example 1, the 2 nd patient takes the medicine provided in example 2, the 3 rd patient takes the medicine provided in example 4, the 4 th patient takes the medicine provided in example 5, the 5 th patient takes the medicine provided in example 8, and the 6 th patient takes the medicine provided in example 10.

The American ginseng, salvia miltiorrhiza and pseudo-ginseng pills are prepared according to the method provided by the embodiment 11.

The dosage is as follows: 3g each time, twice a day; after 30 days of administration, blood samples were taken and analyzed, and the results are shown in Table 4; other symptoms of the patient were also recorded and observed, and the results are shown in table 5.

TABLE 4 analysis of blood sample indices before and after administration

Note: PLG: a-plasma plasminogen activity, TM-thrombomodulin.

TABLE 510 comparison of other symptoms for patients

	Patient condition	Before taking	After taking
				Patient 1	Female, age 35	Chest oppression and limp limbs	No discomfort, vigorous body, and reduced chest distress
Patient 2	Male, age 46	Forgetting something and dreaming	No discomfort, improved memory, and less dreaminess
				Patient 3	Male, age 32 years old	Insomnia, headache and mental confusion	No discomfort, no headache, good sleep, and tranquilization
Patient 4	Female, 56 years old	Dizziness and lassitude in the four limbs	No discomfort, obvious improvement of dizziness and improvement of vitality
				Patient 5	Female, 47 years old	Chest distress and insomnia	No discomfort, smooth heart-qi and good sleep
Patient 6	Male, age 60	Poor mnemonic, restlessness and restlessness	No discomfort, good memory, calm the mind, calm the heart and calm the mind

In general, plasmatic plasminogen active PLG: a is in the normal range of 0.7-1.4; the normal value of thrombomodulin TM is 0.82-1.13; the normal value of the platelet aggregation rate is 50-84%.

From the data in table 4, the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the invention can reduce the platelet aggregation rate of a patient with thrombus, so that the patient can recover to a normal level, the characteristics of mutual adhesion among platelets are reduced, and the thrombus is not easy to form; plasma plasminogen active PLG: a is reduced, which shows that the fibrinolytic activity of blood is good, the thrombomodulin recovers to the normal level, the thrombus condition of a patient is obviously improved, and the formation of thrombus is effectively inhibited.

Meanwhile, in combination with the accompanying symptoms of the patients in table 5, the American ginseng, salvia miltiorrhiza and pseudo-ginseng pill provided by the invention has the effects of calming the nerves and calming the nerves, eliminating fatigue, enhancing memory and improving sleep quality, is free from discomfort, safe and free from side effects, and can effectively relieve other symptoms caused by thrombus of the patients.

Claims

1. The American ginseng, salvia miltiorrhiza and pseudo-ginseng pill is characterized in that: the pill comprises radix Panacis Quinquefolii, Saviae Miltiorrhizae radix, Notoginseng radix, Bombyx Batryticatus and Hirudo; the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 0.8-1.2: 0.2-0.5: 0.2 to 0.6.

2. The American ginseng, salvia miltiorrhiza and pseudo-ginseng pill as claimed in claim 1, which is characterized in that: the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 1: 0.2-0.5: 0.2 to 0.6.

3. The American ginseng, salvia miltiorrhiza and pseudo-ginseng pill as claimed in claim 2, which is characterized in that: the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 1: 0.3: 0.2 to 0.6.

4. The American ginseng, salvia miltiorrhiza and pseudo-ginseng pill as claimed in claim 2, which is characterized in that: the mass ratio of the American ginseng, the salvia miltiorrhiza, the pseudo-ginseng, the stiff silkworm and the leech is 1: 1: 1: 0.3: 0.4.

5. a method for preparing the American ginseng, red sage root and pseudo-ginseng pill as claimed in claims 1 to 4, which is characterized by comprising the following steps: the preparation method comprises the following steps:

6. The American ginseng, salvia miltiorrhiza and pseudo-ginseng pill as claimed in claims 1 to 4, which is characterized in that: the dosage of the pill is 3-6g for each time, and 2 times per day.

7. An application of the American ginseng, red sage root and pseudo-ginseng pill as claimed in claims 1-4 in improving thrombofibrinolysis.

8. An application of the American ginseng, red sage root and pseudo-ginseng pill as claimed in claims 1-4 in improving anoxia resistance of human body.