CN102771391A - Forcing culture technique of virus-free lily by industrial tissue culture and low-temperature bulb treatment - Google Patents
Forcing culture technique of virus-free lily by industrial tissue culture and low-temperature bulb treatment Download PDFInfo
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- CN102771391A CN102771391A CN2012102468233A CN201210246823A CN102771391A CN 102771391 A CN102771391 A CN 102771391A CN 2012102468233 A CN2012102468233 A CN 2012102468233A CN 201210246823 A CN201210246823 A CN 201210246823A CN 102771391 A CN102771391 A CN 102771391A
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Abstract
The invention discloses a virus-free rapid propagation technique of edible lily to solve the problem that culture of producing bulbs with virus-free tissue culture bulbs of edible lily is long in cycle. The technique includes planting lily bulbs more than 2.2cm in length, subjecting the bulbs to vernalization by a 4 DEG C refrigerator for 40-45 days, and thereby shortening culture time of the edible lily from 5 years to 3 years. Culture steps sequentially include culture of edible lily stem tip, subculture, strong seedling culture, bulb induction, bulb vernalization, soil culture, and bulb harvesting. The whole production process of the lily bulbs is adjusted and optimized, the culture process of the virus-free bulbs is simplified, the culture time of the virus-free bulbs of the edible lily is shortened greatly, production cost is lowered while bulb quality is improved, market competitiveness of the edible lily bulbs is enhanced, and the technique is applicable to large-scale production of the virus-free bulbs of the edible lily.
Description
Technical field
The present invention relates to a kind of edible detoxification lily forcing culture technology, training batch production of especially edible detoxification lily group and kind ball low temperature treatment thereof promote culture technique.
Background technology
Edible lily is at the cultivation history in existing nearly thousand of China, and according to the Compendium of Material Medica record, lily has the effect of " feeling at ease to decide courage, intelligence development, foster the five internal organs ".Wherein, Multiple compositions such as bulb rich in proteins, starch, fat and the alkaloid of lily; Nutritive value is very high, can play clearing heat and detoxicating, moisten the lung and relieve the cough, tonifying middle-Jiao and Qi, raising immunity, detumescence is anticancer and effects such as beauty treatment, so be used as good nourishing food by people always.Because edible lily is precious from head to foot, when the market demand increased day by day at home, American-European market and Southeast Asian countries were also progressively recognized its value.Conventional lily propagation method is generally the clove breeding, and the reproduction coefficient of this propagation method is lower, and easily infected virus has a strong impact on its economic worth simultaneously.
The distribution of lily disease viral disease is wider, and it is more serious to fall ill, and is one of main disease during edible lily production and fresh cut-flowers are produced.Simultaneously, the height of the survival rate of lily lepisphere and the rate of putting forth then also is the major issue that influences lily output always.On producing, the conventional method of edible lily kind ball breeding is: adopting the scale cutting propagation is clove, plants in the field afterwards again and could cultivate into commodity kind ball about 2 years.Because this traditional modes of reproduction very easily infected virus is sick, and parasitic insect pest also is prone to propagate on the scale.Therefore, adopting tissue culture mode to breed in the lily ball production at home and abroad of this technology of healthy eating lily material is used widely.Behind tissue culture mode acquisition test tube lepisphere, the general qualified products that just can become lily ball again through the field cultivation of 3-5.In the production practices of reality, very low less than bar (stem) rate of taking out after 2.2 centimeters the edible test tube lily lepisphere field planting, do not take out bar (stem); Linear leaf, a little less than the bulb growth gesture, long very little of underground then lepisphere; Distinguish does not need just can reach criterion of acceptability through 5 years.And the test tube lepisphere plant vegetative growth that can normally take out bar (stem) is vigorous, and the cycle of cultivating qualified kind of ball only needs 2-3, therefore promotes lepisphere normally to take out bar (stem) technology and in edible lily production, is significant.
Patent No. ZL03135142.5 and CN101233826A disclose the detoxifying fast breeding method of edible lily; Mainly concentrate on fast numerous technology; The present invention lays particular emphasis on lily group training batch production lepisphere forcing culture technology; Can edible lily detoxifying kind ball be shortened to 3 years to producing the plantation speed of planting by 5 years, have certain practical value.
Summary of the invention
Technical problem to be solved by this invention is to provide the training batch production of a kind of detoxification lily group and plants ball low temperature treatment forcing culture technology; Can significantly improve edible lily lepisphere survival rate and take out bar (stem) rate; Can obtain the lily bulbil then, shorten the production cycle.
The present invention solves the problems of the technologies described above the technical scheme of being taked:
The detoxifying fast breeding technique of edible lily is characterized in that following these steps to carrying out:
Get the edible lily stem apex through start, subculture strong sprout; Obtain detoxification edible lily tissue cultivating seedling, through detect be virus-free seedling after, through behind the strong sprout regeneration induction lepisphere; Choose the lepisphere more than 2.2 centimeters; Through low temperature 3-5 ℃ after vernalization 40-50 days, plant to matrix put forth the then growing state of situation and underground lepisphere of observed and recorded edible lily.
Described detoxification tissue-cultured derived plant lily is cultivated and is comprised the steps:
The first step is got edible lily kind ball, and the stem apex that under anatomical lens, strips the 0.15mm-0.2mm size is as expanding numerous starting material;
Second step; Little stem apex is inoculated in and starts medium MS+6-BA 0.2-0.5mg/l+ sucrose 3-5%+ agar 5-8g/l; Cultivate on the medium of PH=5.8, secretly cultivate to change under the light after 3-4 days and cultivate, cultivation temperature is 24 ± 2 ℃; Intensity of illumination is 2000-3000 LX, and light application time is 12-14h/ days.
Described subculture strong sprout is at subculture medium MS+6-BA 1-3mg/l+NAA 0.01-0.02 mg/l+ coconut milk 10-20%+ sucrose 3-5%+ agar 5-8g/l with detoxification edible lily tissue cultivating seedling; Subculture, strong sprout on the medium of PH=5.8; Cultivation temperature is 24 ± 2 ℃; Intensity of illumination is 2000-3000 LX, and light application time is 12-14h/ days.
Described regeneration induction lepisphere be with the Cheng Miao of said subculture after strong sprout at root media MS+NAA 0.01-0.03mg/l+ sucrose 10-15%+ agar 6-8g/l, the last regeneration induction lepisphere of PH=5.8, cultivation temperature is 24 ± 2 ℃, low-light was cultivated 40-45 days.
Described lepisphere more than 2.2 centimeters lies against in the porcelain dish behind clear water flush away agar, covers to keep certain humidity with wet gauze.
Lepisphere after the said vernalization insect protected isolate and the condition of taking shelter from rain under plant the matrix peat of sterilization: among perlite=1-3:1; Beginning rudiment after 10-15 days emerges; Can take out bar (stem) then, and bloom, balling, shorten the edible lily kind ball production time.
It is 15-20 ℃ that described lepisphere requires temperature in setting date to the seeding stage, suitably shades; Require temperature at 15-25 ℃ after emerging, regularly spray Chinese medicine preventing disease and pest carries out the control of damage by disease and insect.The agent of described Chinese medicine preventing disease and pest is by following raw materials in part by weight: 8-10 part rhizoma nardostachyos, 8-10 part root of langdu, 10-12 part tuber of stemona, 8-10 part wilson passionflower herb, 8-10 part Swamp Mahogany Leaf add decocting and boil, collect filtrating and be condensed into dried powder and process, and convert 300-400 times of water during use.
Beneficial effect of the present invention is: through the production routine of whole lily ball being adjusted and being optimized; Simplified the cultivation program of detoxification kind ball; Shortened the cultivation time of edible lily detoxifying kind ball greatly; When reducing production costs, improve kind ball quality, strengthened the market competitiveness of edible lily kind ball, be applicable to the large-scale production of edible lily detoxifying kind ball.
Embodiment
Through following examples the present invention is done further detailed description.
Embodiment: improve put forth the then method of rate of edible lily test tube bulbs.
Get the edible lily stem apex through start, subculture strong sprout; Obtain detoxification edible lily tissue cultivating seedling, through detect be virus-free seedling after, through behind the strong sprout regeneration induction lepisphere; Choose the lepisphere more than 2.2 centimeters; Through low temperature 3-5 ℃ after vernalization 40-50 days, plant to matrix put forth the then growing state of situation and underground lepisphere of observed and recorded edible lily.
Concrete grammar carries out according to the following steps:
1, get edible lily kind ball, the stem apex that under anatomical lens, strips the 0.2mm size is as expanding numerous starting material.
2, little stem apex is inoculated in and starts medium MS+6-BA0.2mg/l+ sucrose 3%+ agar 7g/l, cultivates on the medium of PH 5.8, secretly cultivates to change under the light after 3-4 days and cultivates, and cultivation temperature is 24 ℃, and intensity of illumination is 2500 LX, and light application time is 14h/ days.
3, treat that stem apex is grown up after; With detoxification edible lily tissue cultivating seedling at subculture medium MS+6-BA1.5mg/l+NAA 0.01 mg/l+ coconut milk 15%+ sucrose 3%+ agar 7g/l; Subculture, strong sprout on the medium of PH 5.8; Cultivation temperature is 24 ℃, and intensity of illumination is 2500 LX, and light application time is 14h/ days.
4, the regeneration induction lepisphere be with the Cheng Miao of subculture after strong sprout at root media MS+NAA 0.02 mg/l+ sucrose 10%+ agar 7g/l, regeneration induction lepisphere on the medium of PH 5.8, cultivation temperature is 24 ℃, low-light was cultivated 45 days.
5, choose edible lily lepisphere, lepisphere through clear water flush away agar, is lain against in the porcelain dish, cover to keep certain humidity, place 4 ℃ of refrigerators to carry out the low temperature vernalization 45 days with wet gauze greater than 2.2 centimeters.
6, with the lepisphere after the vernalization insect protected isolate and the condition of taking shelter from rain under plant the matrix peat of sterilization: among perlite=3:1, begin rudiment after 15 days and emerge, can put forth then, and bloom, balling, shorten the edible lily kind ball production time.
7, the lily culture administrative skill after the field planting is: it is 20 ℃ that setting date to the seeding stage requires temperature, suitably shades; Require temperature at 25 ℃ after emerging, the control of damage by disease and insect is carried out in spraying pesticide or the agent of Chinese medicine damage by disease and insect regularly.
The agent of described Chinese medicine preventing disease and pest is by following raw materials in part by weight: 8 parts of rhizoma nardostachyos, 8 parts of roots of langdu, 12 parts of tubers of stemona, 10 parts of wilson passionflower herbs, 10 parts of Swamp Mahogany Leafs add decocting and boil, collect filtrating and be condensed into dried powder and process, and convert 300-400 times of water during use.
The present invention studied lepisphere order of magnitude, low temperature vernalization time to the edible lily survival rate, take out the influence of bar (stem) rate and bulbil growth, wherein,
(1) lepisphere order of magnitude: lepisphere is divided into the 0-1 centimetre, the 1-2 centimetre, the 2-3 centimetre, four grades more than 3 centimeters, the lepisphere survival rate and the rates of putting forth of relatively more different sizes, the result when lepisphere survival rate and the rate of putting forth more than 2 centimeters the time all the highest.
(2) the low temperature vernalization time: place 4 ℃ of refrigerators to handle 35 days the lepisphere more than 2 centimeters, 40 days and 45 days, after comparative result was vernalization in 45 days, taking out bar stem rate can reach more than 95%.
Claims (7)
1. the detoxifying fast breeding technique of an edible lily is characterized in that following these steps to carrying out:
Get the edible lily stem apex through start, subculture strong sprout; Obtain detoxification edible lily tissue cultivating seedling, through detect be virus-free seedling after, through behind the strong sprout regeneration induction lepisphere; Choose the lepisphere more than 2.2 centimeters; Through low temperature 3-5 ℃ after vernalization 40-50 days, plant to matrix put forth the then growing state of situation and underground lepisphere of observed and recorded edible lily.
2. according to the detoxifying fast breeding technique of claims 1 described edible lily, it is characterized in that: described detoxification tissue-cultured derived plant lily is cultivated and is comprised the steps:
The first step is got edible lily kind ball, and the stem apex that under anatomical lens, strips the 0.15mm-0.2mm size is as expanding numerous starting material;
Second step; Little stem apex is inoculated in and starts medium MS+6-BA 0.2-0.5mg/l+ sucrose 3-5%+ agar 5-8g/l; Cultivate on the medium of PH=5.8, secretly cultivate to change under the light after 3-4 days and cultivate, cultivation temperature is 24 ± 2 ℃; Intensity of illumination is 2000-3000 LX, and light application time is 12-14h/ days.
3. the detoxifying fast breeding technique of edible lily according to claim 1; It is characterized in that: described subculture strong sprout for detoxification edible lily tissue cultivating seedling at subculture medium MS+6-BA 1-3mg/l+NAA 0.01-0.02 mg/l+ coconut milk 10-20%+ sucrose 3-5%+ agar 5-8g/l; Subculture, strong sprout on the medium of PH=5.8; Cultivation temperature is 24 ± 2 ℃, and intensity of illumination is 2000-3000 LX, and light application time is 12-14h/ days.
4. the detoxifying fast breeding technique of edible lily according to claim 1; It is characterized in that: described regeneration induction lepisphere is at root media MS+NAA 0.01-0.03mg/l+ sucrose 10-15%+ agar 6-8g/l with the Cheng Miao of said subculture after strong sprout; The last regeneration induction lepisphere of PH=5.8; Cultivation temperature is 24 ± 2 ℃, and low-light was cultivated 40-45 days.
5. the detoxifying fast breeding technique of edible lily according to claim 1, it is characterized in that: described lepisphere more than 2.2 centimeters lies against in the porcelain dish behind clear water flush away agar, covers to keep certain humidity with wet gauze.
6. the detoxifying fast breeding technique of edible lily according to claim 1; It is characterized in that: the lepisphere after the said vernalization insect protected isolate and the condition of taking shelter from rain under plant the matrix peat of sterilization: among perlite=1-3:1; Beginning rudiment after 10-15 days emerges; Can take out bar (stem) then, and bloom, balling, shorten the edible lily kind ball production time.
7. the detoxifying fast breeding technique of edible lily according to claim 5 is characterized in that: it is 15-20 ℃ that described lepisphere requires temperature in setting date to the seeding stage, suitably shades; Require temperature at 15-25 ℃ after emerging; Regularly spray Chinese medicine preventing disease and pest carries out the control of damage by disease and insect; The agent of described Chinese medicine preventing disease and pest is by following raw materials in part by weight: 8-10 part rhizoma nardostachyos, 8-10 part root of langdu, 10-12 part tuber of stemona, 8-10 part wilson passionflower herb, 8-10 part Swamp Mahogany Leaf add decocting and boil, collect filtrating and be condensed into dried powder and process, and convert 300-400 times of water during use.
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Cited By (7)
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| CN103875432A (en) * | 2014-04-09 | 2014-06-25 | 南京工业大学大丰海洋产业研究院 | Method for preparing seed balls through low-temperature treatment of cut saffron crocus blocks |
| CN104186319A (en) * | 2014-08-19 | 2014-12-10 | 上海市农业科学院 | Lily root tip detoxification and rapid propagation method for lily |
| TWI507123B (en) * | 2013-09-25 | 2015-11-11 | Ko Nan Industry Co Ltd | Sustainable process for bulb propagation |
| CN106973770A (en) * | 2017-04-18 | 2017-07-25 | 四川农业大学 | A kind of cultural method of Fragrant Lily |
| CN107548991A (en) * | 2017-09-28 | 2018-01-09 | 福建省农业科学院生物技术研究所 | A kind of inoculation method of lily test tube bulbs |
| CN112293250A (en) * | 2019-08-02 | 2021-02-02 | 伽蓝（集团）股份有限公司 | Culture method of nardostachys chinensis callus cells |
| CN115968737A (en) * | 2023-02-03 | 2023-04-18 | 湖南岳麓山中药材种业创新中心有限公司 | Out-of-season green cultivation method and harvesting device for longya lilies |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI507123B (en) * | 2013-09-25 | 2015-11-11 | Ko Nan Industry Co Ltd | Sustainable process for bulb propagation |
| CN103875432A (en) * | 2014-04-09 | 2014-06-25 | 南京工业大学大丰海洋产业研究院 | Method for preparing seed balls through low-temperature treatment of cut saffron crocus blocks |
| CN103875432B (en) * | 2014-04-09 | 2016-02-24 | 南京工业大学大丰海洋产业研究院 | The method that son plants ball is prepared in the stripping and slicing of low temperature treatment safflower |
| CN104186319A (en) * | 2014-08-19 | 2014-12-10 | 上海市农业科学院 | Lily root tip detoxification and rapid propagation method for lily |
| CN104186319B (en) * | 2014-08-19 | 2016-05-11 | 上海市农业科学院 | Tip of a root detoxification and the quick-breeding method of a kind of lily |
| CN106973770A (en) * | 2017-04-18 | 2017-07-25 | 四川农业大学 | A kind of cultural method of Fragrant Lily |
| CN107548991A (en) * | 2017-09-28 | 2018-01-09 | 福建省农业科学院生物技术研究所 | A kind of inoculation method of lily test tube bulbs |
| CN107548991B (en) * | 2017-09-28 | 2019-07-23 | 福建省农业科学院生物技术研究所 | A kind of inoculation method of lily test tube bulbs |
| CN112293250A (en) * | 2019-08-02 | 2021-02-02 | 伽蓝（集团）股份有限公司 | Culture method of nardostachys chinensis callus cells |
| CN112293250B (en) * | 2019-08-02 | 2023-02-17 | 伽蓝（集团）股份有限公司 | Culture method of nardostachys chinensis callus cells |
| CN115968737A (en) * | 2023-02-03 | 2023-04-18 | 湖南岳麓山中药材种业创新中心有限公司 | Out-of-season green cultivation method and harvesting device for longya lilies |
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