CN102757907A - Endosulfan degradation stain and application thereof in soil remediation - Google Patents
Endosulfan degradation stain and application thereof in soil remediation Download PDFInfo
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Abstract
The invention discloses an endosulfan degradation stain and application thereof in soil remediation. The endosulfan degradation stain is a strain LD-6 of Stenotrophomonas rhizophila, is preserved in China general microbiological strain preservation administration center On March 2, 2012, and the preservation number is CGMCC No.5839. The endosulfan degradation stain can realize the complete degradation of 100 mg/L of endosulfan within 10 days under an aerobic condition and has low-toxic or non-toxic degradation products and fast environmental degradation; the strain LD-6 is sensitive to common antibiotics, such as tetracycline and kanamycin and tolerant to 13 tested heavy metals and can inhibit the growth of a plurality of phytopathogens; and the endosulfan degradation stain has capability of efficiently degrading the endosulfan in an actual soil environment, can outstandingly shorten the degrading time of the endosulfan and has practical application significance and value.
Description
Technical field
The invention belongs to environmental pollutant biologic treating technique field, be specifically related to the bacterium of a high-efficiency degradation 5a,6,9,9a-hexahydro-6,9-methano-2,4 and pollute the application in repairing in edatope.
Background technology
Agricultural chemicals is being brought into play important role as a kind of important agricultural material at aspects such as protection agriculture prodn, raising overall productivity in agriculture, the raising the output of promotion stable food and peasant continue to increase income.China is pesticide producing big country, also is to use big country, and year usage quantity is about 1,200,000 tons and 2.34 kg/ha respectively with average consumption, ranks the first in the world.Yet the utilization ratio of agricultural chemicals but is merely 10-20%, and rest part all gets into soil, water body and atmosphere, has caused serious pollution.According to statistics, the whole nation receives the farmland of pesticide residual contamination that ten thousand hectares of 1300-1600 are arranged approximately, accounts for and can plough 10% of area; The nearly half vegetable pesticide residue exceeds standard, and suffer exit loss nearly 80 hundred million dollar because of the residual problem of exceeding standard of farming every year.
China begins to develop organochlorine pesticide from the 50's of 20th century, mainly comprises products such as phenyl-hexachloride, compound 118, Dieldrin-attapulgite mixture, Niran and heptachlor, and they once brought into play significant role in industrial and agricultural production.But because this compounds has extremely strong inrichment in food chain, be important environmental classes hormone and persistence organic pollutant, major part has stopped producing and using.So far, this pesticide residue is still constantly threatening national health, the benign development of restriction rural economy.
5a,6,9,9a-hexahydro-6,9-methano-2,4 (Endosulfan) is made up of α and b 5a,6,9,9a-hexahydro-6,9-methano-2,4; Commodity match by name red (Thionex), large pellet (Thiodan); Be a kind of high-efficiency broad spectrum organochlorine insecticide, relative low price is widely used in the pest controls of crops such as tealeaves, cotton, fruit tree and vegetables.5a,6,9,9a-hexahydro-6,9-methano-2,4 belongs to riskiest pesticide, and is poisonous to animal especially fish, can damage cns, immunity system, the reproductive system of animal, and organs such as brain, liver and kidney.The 5a,6,9,9a-hexahydro-6,9-methano-2,4 residual period, is long, is one of several verieties that the environment residual concentration is the highest in the organochlorine insecticide, has bioconcentration, and the transformation period in soil reaches 6-11 month.
At present, through technology such as artificial enrichment culture, many bacterium and fungies have been isolated with degraded organochlorine pesticide compound ability.Wherein, bacterium is owing to have that adaptive faculty is strong, breeding is fast and the characteristics such as quick of degrading occupy main status.Yet; The efficient degrading bacteria of the tool 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation capability that has been separated to is still very limited; Most bacterial strains can't be realized the degraded fully to 100 mg/L 5a,6,9,9a-hexahydro-6,9-methano-2,4s in 10 days, and mostly degraded product is the 5a,6,9,9a-hexahydro-6,9-methano-2,4 vitriol (Endosulf sulfate) that toxicity is bigger, residual period is longer and environmental hazard is bigger.People such as Li Wen once reported the efficient pale bacillus (C7) of a strain tool 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation capability, but this bacterial strain to the degradation capability of 50 mg/L 5a,6,9,9a-hexahydro-6,9-methano-2,4s about 90.2%, (degradation rate is mg/L every days 5.64) (Li Wen can't realize degrading fully; Peng Xiang; Zhang Jingshun, Zhang Chen, Yan Yanchun.The screening of 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria and degradation property research.The Shandong agricultural sciences, 2009,1:67-70).Reported that the highest bacterial strain of degradation rate is viride (Trichoderma viride) H082, this bacterial strain can be in 7 days efficient degradation 100 mg/L 5a,6,9,9a-hexahydro-6,9-methano-2,4s, degradation rate reaches 95% (Zhu Lihong, Wu Jian, Sun Dongchang, Shi Yuefeng.The separation of 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria H082, screening and bacteriostasis property research), but can't realize degrading fully.In addition, bacterial strain H082 belongs to filamentous fungus, compares with bacterial isolates, and obviously not enough below existing: 1) growth velocity is slow.The bacterium industrial fermentation is generally between 2-3 days, and the filamentous fungus fermentation then is no less than 4, how between 6-8 day.With regard to the general industry fermentation, the ferment product cell concentration can reach 1-5 * 10
9CFU/mL (CFU, colony-forming unit), fungus solids fermentation spore count then will hang down an one magnitude; 2) the fermentation equipment versatility is poor.Filamentous fungus fermentation solid fermentation bed or the fermentor tanks of adopting, equipment interoperability is poor more.Fermentation using bacteria also can be used for secondary metabolite production with fermentor tank except that producing the thalline, like microbiotic, pigment and VITAMINs etc., universal performance is good; 3) the microbial inoculum application effect is slow relatively.After the bacterium microbial inoculum sprayed, bacterium directly acted on pesticide residue, and the filamentous fungus spore then need be treated can act on behind sprouting, the formation mycelia.In addition, the 5a,6,9,9a-hexahydro-6,9-methano-2,4 meta-bolites of above-mentioned two strain degradation bacteria, antibiotics resistance and heavy metal tolerance etc. are not all verified as yet, exist certain environment to use risk.In sum, screening is efficient, the tolerance heavy metal, to antibiotic sensitive, and the 5a,6,9,9a-hexahydro-6,9-methano-2,4 bacterium for degrading of the non-5a,6,9,9a-hexahydro-6,9-methano-2,4 vitriol of degraded product becomes the research focus.
Summary of the invention
The objective of the invention is in 10 days, to realize degraded fully to what 5a,6,9,9a-hexahydro-6,9-methano-2,4 degraded existed to 100 mg/L 5a,6,9,9a-hexahydro-6,9-methano-2,4s to above-mentioned existing 5a,6,9,9a-hexahydro-6,9-methano-2,4 mikrobe; And mostly degraded product is the defective of the 5a,6,9,9a-hexahydro-6,9-methano-2,4 vitriol (Endosulf sulfate) that toxicity is bigger, residual period is longer and environmental hazard is bigger, and a kind of efficient 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria is provided.
Another object of the present invention provides the application of above-mentioned bacterial classification in repairing the 5a,6,9,9a-hexahydro-6,9-methano-2,4 contaminated soil.
Above-mentioned technical problem of the present invention is able to implement through following technical scheme:
One strain 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria; This bacterium is the bacterial strain LD-6 that has a liking for root oligotrophy Zymomonas mobilis (Stenotrophomonas rhizophila); In on March 2nd, 2012 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation; Be numbered CGMCC No.5839, this bacterium has the ability of efficient degradation 5a,6,9,9a-hexahydro-6,9-methano-2,4.The address of depositary institution is No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and the classification called after of this bacterium is had a liking for root oligotrophy Zymomonas mobilis Stenotrophomonas rhizophila.
The application of a kind of said 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria in receiving the reparation of 5a,6,9,9a-hexahydro-6,9-methano-2,4 contaminated soil.
As preferably, said bacterial strain CGMCC No.5839 is seeded in the basic inorganic salt liquid substratum that contains 5a,6,9,9a-hexahydro-6,9-methano-2,4 50-100 mg/L, 120 r/min concussion is cultivated more than the 48h, and described basic inorganic salt liquid substratum composition is (g/L): MgCl
20.2, NH
4NO
31, KH
2PO
42, K
2HPO
47.5 NaCl 1,5a,6,9,9a-hexahydro-6,9-methano-2,4 0.1, deionized water 1 L.
As preferably, described liquid culture temperature is 25-30 ℃, and the pH of basic inorganic salt liquid substratum is 5.4-6.8.
As preferably, described liquid culture temperature is 30 ℃.
As preferably, said bacterial strain CGMCC No.5839 is seeded to 30 ℃ of constant temperature culture obtained the thalline fermented liquid more than 24 hours in the fermention medium, degrade to receiving the 5a,6,9,9a-hexahydro-6,9-methano-2,4 Contaminated soil to spray the thalline fermented liquid; The composition of fermention medium is (g/L): Semen Maydis powder 10 g/L, bean cake powder 8 g/L, (NH
4)
2SO
46 g/L, steeping water 6 g/L, MgSO
47H
2O 1 g/L, K
2HPO
43H
2O 2 g/L, MnSO
40.05 g/L, pH 7.2.
5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria provided by the present invention
Stenotrophomonas rhizophilaLD-6 is derived near the mud of Tonglu, Zhejiang insecticide factory sewage draining exit, obtains through artificial enrichment culture, pressure screening and separation and purification.This Pseudomonas is had a liking for root oligotrophy Zymomonas mobilis, and its phylogeny analysis on Position is as shown in Figure 1.Its physiological and biochemical property is: Gram-negative, and catalase is positive, oxidase negative; V. the P. test is negative, and indole test is negative, and obligate is supported well; Can be 4 ℃ of growths, but can't survive in 40 ℃, can with the wood sugar carbon source; Thalli morphology is a rod-short, and that bacterium colony is is light yellow, circular, the edge is neat, smooth moistening, and the GenBank accession number of bacterial strain 16S rDNA is JQ670922; In on March 2nd, 2012 in the preservation of Chinese common micro-organisms culture presevation administrative center, be numbered CGMCC No.5839.
The optimum growing condition of this bacterium is: pH 7.2, and temperature is 30 ℃.With 1/5th LB (Luria-Bertani) or industrial fermentation substratum (Semen Maydis powder 10 g/L, bean cake powder 8 g/L, (NH
4)
2SO
46 g/L, steeping water 6 g/L, MgSO
47H
2O 1 g/L, K
2HPO
43H
2O 2 g/L, MnSO
40.05 g/L, pH 7.2) to cultivate after 24-36 hour, bacterial concentration can reach 5-8 * 10
9CFU/mL.
This bacterium is responsive to tsiklomitsin and kantlex, and the two is all common antibiotics.Can think: when thalline is released in the physical environment, can be because of not resisting (anti-) property of medicine problem to produce superbacteria or between indigenous bacterium, propagating.
This bacterium to Candida albicans (
Candida albicans), dry thread Pyrenomycetes (
Rhizoctonia solani), sclerotinite (
Sclerotinia sclerotiorum), verticillium wilt pathogen (
Verticillium dahliae), fusarium graminearum (
Fusarium graminearum) and the capsicum wilt (
Fusarium oxysporum) present obvious restraining effect.Can think: after thalline is used, can play the effect that suppresses or alleviate the Plant diseases generation to a certain extent.
This bacterium is to 13 kinds of test metals ion (Li
+, Ag
+, Cu
+, Cu
2+, Hg
2+, Ba
2+, Pb
2+, Zn
2+, Mn
2+, Ni
2+, Fe
2+, Fe
3+And Co4
+) present tolerance in various degree.In view of China's soil pollution present situation---persistence organic pollutant pollution and heavy metal contamination are also deposited, it is very important that the pesticide residual degradation bacterial strain possesses heavy metal tolerance performance.Can think: under the general soil envrionment conditions, this thalline is used repairing effect and is received heavy metal to suppress to influence less.
(volume is the triangular flask of 250 mL to this bacterial strain, and 100 mL liquid minimal mediums are housed, and 5a,6,9,9a-hexahydro-6,9-methano-2,4 concentration is 100 mg/L in shaking bottle degraded test; Shaking speed is 160 r/min; Temperature is 30 ℃), can in 10 days, 5a,6,9,9a-hexahydro-6,9-methano-2,4 be degraded fully (degradation rate is mg/L every days 10), meta-bolites is 5a,6,9,9a-hexahydro-6,9-methano-2,4 ether and 5a,6,9,9a-hexahydro-6,9-methano-2,4 glycol; The two toxicity is much smaller than 5a,6,9,9a-hexahydro-6,9-methano-2,4, and environment degradable is quick.At soil 5a,6,9,9a-hexahydro-6,9-methano-2,4 content is under the 50 mg/kg situation, uses this bacterium and can make in 28 days and supply in the examination soil sample 68.6% 5a,6,9,9a-hexahydro-6,9-methano-2,4 to degrade, and significantly promotes the 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation rate, shortens the duration of degrading, and possesses practical application meaning and value.
This bacterium is to have reported in the degradation bacteria, and what a unique strain possessed the 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation capability has a liking for root oligotrophy Zymomonas mobilis.
In sum, the present invention compares with prior art and has following advantage:
Compared with prior art, of the present invention
Stenotrophomonas rhizophilaLD-6 be first strain possess the 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation capability have a liking for root oligotrophy Zymomonas mobilis, can under aerobic condition, carry out growth and breeding as sole carbon source and sulphur source, simultaneously with its quick degraded with 5a,6,9,9a-hexahydro-6,9-methano-2,4.Under the pure culture condition, it can be that the 5a,6,9,9a-hexahydro-6,9-methano-2,4 of 100 mg/L was degraded in 10 days fully with concentration.Compare with existing 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria strains; This bacterium is when possessing higher 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation rate (being only second to above-mentioned viride H082); Responsive to common antibiotics, can tolerate 13 heavy metal species, there is restraining effect in the various plants pathogenic micro-organism; Degraded product low toxicity or nontoxic; The industrial scale fermentative prodn is convenient, fermented liquid cell concentration high (being superior to viride H082), and possesses the ability of performance 5a,6,9,9a-hexahydro-6,9-methano-2,4 Degradation under actual edatope condition, and this bacterial strain is expected in the coenocorrelation reparation of 5a,6,9,9a-hexahydro-6,9-methano-2,4 contaminated soil, play a significant role.
Description of drawings
The phylogeny analysis on Position of Fig. 1, bacterial strain LD-6.
The degradation capability test of Fig. 2, bacterial strain LD-6, wherein ■: α 5a,6,9,9a-hexahydro-6,9-methano-2,4; ▲: the b 5a,6,9,9a-hexahydro-6,9-methano-2,4; ◆: α and b 5a,6,9,9a-hexahydro-6,9-methano-2,4 sum.
5a,6,9,9a-hexahydro-6,9-methano-2,4 degraded situation, wherein in Fig. 3, the bacterial strain LD-6 edatope: α 5a,6,9,9a-hexahydro-6,9-methano-2,4 content (contrast) in the deactivation soil sample; ■: spray α 5a,6,9,9a-hexahydro-6,9-methano-2,4 content in the LD-6 thalline deactivation soil sample; △: α 5a,6,9,9a-hexahydro-6,9-methano-2,4 content (contrast) in the fresh soil sample; ▲: spray α 5a,6,9,9a-hexahydro-6,9-methano-2,4 content in the LD-6 thalline fresh soil sample; ◇: b 5a,6,9,9a-hexahydro-6,9-methano-2,4 content (contrast) in the deactivation soil sample; ◆: spray b 5a,6,9,9a-hexahydro-6,9-methano-2,4 content in the LD-6 thalline deactivation soil sample; Zero: b 5a,6,9,9a-hexahydro-6,9-methano-2,4 content (contrast) in the fresh soil sample; ●: spray b 5a,6,9,9a-hexahydro-6,9-methano-2,4 content in the LD-6 thalline fresh soil sample.
Embodiment
Through specific embodiment, technical scheme of the present invention is further specified below.Should be appreciated that enforcement of the present invention is not limited to following embodiment, all will fall into protection domain of the present invention any pro forma accommodation and/or the change that the present invention made.
In the present invention, if not refer in particular to, all part, per-cents are weight unit, and all equipment and raw material etc. all can be buied from market or the industry is commonly used.
Embodiment 1: Stenotrophomonas rhizophilaThe separation of LD-6 and 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation capability
Near the collection in worksite Zhejiang Tonglu insecticide factory sewage draining exit mud with 150 mL triangular flask splendid attires, and is taken back the laboratory rapidly.The sample of fetching is placed 5a,6,9,9a-hexahydro-6,9-methano-2,4 selectivity basis inorganic salt liquid substratum acclimation shaking culture.The substratum composition is (g/L): MgCl
20.2, NH
4NO
31, KH
2PO
42, K
2HPO
47.5 NaCl 1,5a,6,9,9a-hexahydro-6,9-methano-2,4 0.1, and deionized water 1 L, 6.8,115 ℃ of moist heat sterilizations of pH are subsequent use after 30 minutes.Culture condition is: 28 ℃, 120 r/min shake cultivation, and the container liquid amount is 1/3 of a vessel volume.After this, will shake a bottle static a moment in every separated 6-7 days, and abandon supernatant, the selected liq substratum of mending the fresh configuration of equivalent subsequently also continues to shake enrichment culture.All around, above-mentioned culture is evenly coated on the solid selectivity basis minimal medium (agar that adds 20 grams in every liter of liquid nutrient medium again).With putting into incubator after the coated flat board inversion, cultivated 72 hours for 28 ℃.With aseptic toothpick select single bacterium colony or with inoculating needle line until occur single bacterium colony again row be forwarded on the solid selectivity flat board of fresh configuration, and verify the 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation capability of each bacterial strain one by one through vapor-phase chromatography, the result obtains a strain 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria LD-6.
With OD
600Value is that 0.5 inoculum size inserts bacterial strain LD-6 with 100 mg/L 5a,6,9,9a-hexahydro-6,9-methano-2,4s is that 30 ℃, 160 r/min shake cultivation in the basic minimal medium of selectivity in sole carbon source and sulphur source.Subsequently, whenever at a distance from 2 days to substratum in the residual condition of 5a,6,9,9a-hexahydro-6,9-methano-2,4 test, the result is as shown in Figure 2.Can find out that therefrom bacterial strain LD-6 can 5a,6,9,9a-hexahydro-6,9-methano-2,4 be to grow in sole carbon source and sulphur source, realizes the degraded fully to 5a,6,9,9a-hexahydro-6,9-methano-2,4 simultaneously.
Present embodiment explanation separation obtains
Stenotrophomonas rhizophilaLD-6 5a,6,9,9a-hexahydro-6,9-methano-2,4 capable of using carries out growth and breeding as sole carbon source and sulphur source, and has the ability of efficient degradation 5a,6,9,9a-hexahydro-6,9-methano-2,4.
Embodiment 2: Stenotrophomonas rhizophilaThe resistance experiment of LD-6
Adopt the filter paper method, select tsiklomitsin and kantlex to test, big or small with each microbiotic filter paper (30 μ g/ sheet) at the antibacterial circle diameter of cultivating on the degradation bacteria LD-6 flat board, as sensitivity or drug-fast criterion.Test-results shows that antibacterial circle diameter is respectively 24 and 16 mm, and (the medium sensitivity scope is: tsiklomitsin 23-25 mm to be the medium sensitivity reaction; Kantlex 14-22 mm).
The present embodiment explanation can be because of resisting (anti-) property of medicine problem to produce superbacteria or between indigenous bacterium, propagating, for its practical application from now on provides safety assurance when thalline is released in the physical environment.
Embodiment 3: Stenotrophomonas rhizophilaHeavy metal minimum inhibition concentration (MIC) test of LD-6
The MIC Test Design is carried out (Filali B.K., Taoufik J., Zeroual Y. with reference to people's such as Filali research; Dzairi F.Z., Talbi M., Blaghen M. Waste water bacterial isolates resistant to heavy metals and antibiotics. Current Microbiology; 2000,41 (3): 151-156), picking etc. are big, the single bacterium colony of the LD-6 of activated processing is in culture test tube; 30 ℃, 160 rpm shaking culture 48 hours.Judge MICs according to the thalline growing way, establish 3 repetitions for every group, test the tolerance situation of this bacterium respectively 13 metal ion species.The result is as shown in table 1, the multiple tolerance of this bacterial strain tool, and tolerance is strong.
Embodiment 4: Stenotrophomonas rhizophilaThe phytopathogen inhibition test of LD-6
With reference to people's reported method such as Zhu Lihong (Zhu Lihong, Wu Jian, Sun Dongchang, Shi Yuefeng.The separation of 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria H082, screening and bacteriostasis property research) carry out the bacteriostasis test of bacterial strain LD-6.The result is as shown in table 2, and this bacterial strain presents restraining effect to multiple confession examination pathogenic bacteria.After present embodiment explanation thalline is used, can play the effect that suppresses or alleviate the Plant diseases generation to a certain extent.
Annotate:+ bacteriostasis rate 50-80%; ++ bacteriostasis rate is more than 80%.
Embodiment 5: Stenotrophomonas rhizophilaThe test of 5a,6,9,9a-hexahydro-6,9-methano-2,4 in the LD-6 degraded soil
Bacterial strain LD-6 30 ℃ of constant temperature culture in fermention medium were obtained the thalline fermented liquid after 24 hours.The composition of fermention medium is (g/L): Semen Maydis powder 10 g/L, bean cake powder 8 g/L, (NH
4)
2SO
46 g/L, steeping water 6 g/L, MgSO
47H
2O 1 g/L, K
2HPO
43H
2O 2 g/L, MnSO
40.05 g/L, pH 7.2.
In sterilized water, add a certain amount of 5a,6,9,9a-hexahydro-6,9-methano-2,4 (5a,6,9,9a-hexahydro-6,9-methano-2,4 is dissolved among the acetone in advance); After fully shaking mixing on the shaking table; Immersion soil to be tried (supplying examination soil is the moisture soil of pH 5.4) makes soil particle can adsorb 5a,6,9,9a-hexahydro-6,9-methano-2,4 uniformly, and 5a,6,9,9a-hexahydro-6,9-methano-2,4 content is 50 mg/kg.Spray the thalline fermented liquid, stir, make that the LD-6 cell concentration is about 1 * 10 in the soil sample
8Individual/g.Soil water content is controlled at about 60%, 25 ℃ of constant temperature.Simultaneously, with reference to people such as Li Wen research (Li W., Dai Y.; Xue B.B., Li Y.Y., Peng X.; Zhang J.S., Yan Y.C. Biodegradation and detoxification of endosulfan in aqueous medium and soil by
Achromobacter xylosoxidansStrain CS5. Journal of Hazardous Materials, 2009,167:209-216), contrast is set.Subsequently, every 5 g soil samples measuring and calculating 5a,6,9,9a-hexahydro-6,9-methano-2,4 degraded situation of getting at a distance from 7 days.
The result is as shown in Figure 3, supplies to have in the examination soil sample 68.6% 5a,6,9,9a-hexahydro-6,9-methano-2,4 to degrade in 28 days, sprays LD-6 and can significantly promote the 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation rate, shorten the duration of degrading, and possesses practical application meaning and value.
Claims (6)
1. a strain 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria, this bacterium be have a liking for root oligotrophy Zymomonas mobilis (
Stenotrophomonas rhizophila) bacterial strain LD-6, on March 2nd, 2012 in the preservation of Chinese common micro-organisms culture presevation administrative center, be numbered CGMCC No.5839, this bacterium has the ability of degraded 5a,6,9,9a-hexahydro-6,9-methano-2,4.
2. the application of the said 5a,6,9,9a-hexahydro-6,9-methano-2,4 degradation bacteria of claim 1 in receiving the reparation of 5a,6,9,9a-hexahydro-6,9-methano-2,4 contaminated soil.
3. the described application of claim 1; It is characterized in that: said bacterial strain CGMCC No.5839 is seeded in the basic inorganic salt liquid substratum that contains 5a,6,9,9a-hexahydro-6,9-methano-2,4 50-100 mg/L; 120 r/min concussion is cultivated more than 48 h, and described basic inorganic salt liquid substratum composition is (g/L): MgCl
20.2, NH
4NO
31, KH
2PO
42, K
2HPO
47.5 NaCl 1,5a,6,9,9a-hexahydro-6,9-methano-2,4 0.1, deionized water 1 L.
4. the described application of claim 3 is characterized in that: described liquid culture temperature is 25-30 ℃, and the pH of basic inorganic salt liquid substratum is 5.4-6.8.
5. the described application of claim 3 is characterized in that: described liquid culture temperature is 30 ℃.
6. the described application of claim 2 is characterized in that: said bacterial strain CGMCC No.5839 is seeded to 30 ℃ of constant temperature culture obtained the thalline fermented liquid more than 24 hours in the fermention medium, degrades to receiving the 5a,6,9,9a-hexahydro-6,9-methano-2,4 Contaminated soil to spray the thalline fermented liquid; The composition of fermention medium is (g/L): Semen Maydis powder 10 g/L, bean cake powder 8 g/L, (NH
4)
2SO
46 g/L, steeping water 6 g/L, MgSO
47H
2O 1 g/L, K
2HPO
43H
2O 2 g/L, MnSO
40.05 g/L, pH 7.2.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103060233A (en) * | 2012-12-27 | 2013-04-24 | 山东农业大学 | A Bordetella petrii NS and applications of an immobilized enzyme thereof in the soil |
| CN105624063A (en) * | 2016-02-04 | 2016-06-01 | 云南圣清环保科技有限公司 | Efficient mixed flora for treating sludge |
| CN106434497A (en) * | 2016-12-12 | 2017-02-22 | 浙江农林大学 | Siderophore high-yielding strain preparation and application of same in remediation of heavy metal contaminated soil |
| CN115094013A (en) * | 2022-08-25 | 2022-09-23 | 江苏聚庚科技有限公司 | Stenotrophomonas rhizophila, microbial inoculum and application of stenotrophomonas rhizophila and microbial inoculum in wastewater treatment |
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2012
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| YU F.B ET AL: "ID:JQ6709221", 《GENBANK》 * |
| 李文等: "硫丹降解菌的筛选及降解性能研究", 《山东农业科学》 * |
| 苏坤昌: "硫丹高效降解菌JBW4的筛选和降解特性研究", 《持久性有机污染物论坛2010 暨第五届持久性有机污染物全国学术研讨会论文集》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103060233A (en) * | 2012-12-27 | 2013-04-24 | 山东农业大学 | A Bordetella petrii NS and applications of an immobilized enzyme thereof in the soil |
| CN103060233B (en) * | 2012-12-27 | 2014-07-23 | 山东农业大学 | A Bordetella petrii NS and applications of an immobilized enzyme thereof in the soil |
| CN105624063A (en) * | 2016-02-04 | 2016-06-01 | 云南圣清环保科技有限公司 | Efficient mixed flora for treating sludge |
| CN106434497A (en) * | 2016-12-12 | 2017-02-22 | 浙江农林大学 | Siderophore high-yielding strain preparation and application of same in remediation of heavy metal contaminated soil |
| CN106434497B (en) * | 2016-12-12 | 2019-06-04 | 浙江农林大学 | Siderophore high yield bacteria preparation and its application in terms of Heavy Metals in Soil Contaminated reparation |
| CN115094013A (en) * | 2022-08-25 | 2022-09-23 | 江苏聚庚科技有限公司 | Stenotrophomonas rhizophila, microbial inoculum and application of stenotrophomonas rhizophila and microbial inoculum in wastewater treatment |
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| CN102757907B (en) | 2013-07-24 |
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