CN116622547A - Bacillus mojavensis YL-78 and application thereof - Google Patents
Bacillus mojavensis YL-78 and application thereof Download PDFInfo
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- CN116622547A CN116622547A CN202310333174.9A CN202310333174A CN116622547A CN 116622547 A CN116622547 A CN 116622547A CN 202310333174 A CN202310333174 A CN 202310333174A CN 116622547 A CN116622547 A CN 116622547A
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- 241001249117 Bacillus mojavensis Species 0.000 title claims abstract description 52
- 239000003513 alkali Substances 0.000 claims abstract description 29
- 230000000813 microbial effect Effects 0.000 claims abstract description 11
- 238000004321 preservation Methods 0.000 claims abstract description 11
- 241000223221 Fusarium oxysporum Species 0.000 claims abstract description 8
- 230000008635 plant growth Effects 0.000 claims abstract description 6
- 230000005764 inhibitory process Effects 0.000 claims abstract description 4
- 238000009629 microbiological culture Methods 0.000 claims abstract description 4
- 230000001580 bacterial effect Effects 0.000 claims description 22
- 238000002360 preparation method Methods 0.000 claims description 14
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 13
- 230000012010 growth Effects 0.000 claims description 11
- 230000001737 promoting effect Effects 0.000 claims description 8
- 238000003794 Gram staining Methods 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
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- 244000052769 pathogen Species 0.000 claims 2
- 230000001717 pathogenic effect Effects 0.000 claims 2
- 244000299906 Cucumis sativus var. sativus Species 0.000 claims 1
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- 239000002689 soil Substances 0.000 abstract description 18
- 244000052616 bacterial pathogen Species 0.000 abstract description 10
- 239000003895 organic fertilizer Substances 0.000 abstract description 9
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 8
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 abstract description 8
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 8
- 239000011574 phosphorus Substances 0.000 abstract description 8
- 229930192334 Auxin Natural products 0.000 abstract description 7
- 241000219109 Citrullus Species 0.000 abstract description 7
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 abstract description 7
- 239000002363 auxin Substances 0.000 abstract description 7
- 150000003839 salts Chemical class 0.000 abstract description 7
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 abstract description 6
- 241000223218 Fusarium Species 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
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- 244000005700 microbiome Species 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 230000002401 inhibitory effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
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- 244000138286 Sorghum saccharatum Species 0.000 description 5
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- 241000287828 Gallus gallus Species 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
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- 210000003608 fece Anatomy 0.000 description 4
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- 239000006228 supernatant Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
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- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
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- 239000002068 microbial inoculum Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
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- 235000019698 starch Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 1
- 239000012880 LB liquid culture medium Substances 0.000 description 1
- WYWFMUBFNXLFJK-UHFFFAOYSA-N [Mo].[Sb] Chemical compound [Mo].[Sb] WYWFMUBFNXLFJK-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
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- 239000008367 deionised water Substances 0.000 description 1
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- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
- C05F3/02—Guano
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C09K2101/00—Agricultural use
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Abstract
The invention belongs to the technical field of agricultural microbial agents, and relates to bacillus mojavensis YL-78 and application thereof. The Bacillus mojavensis YL-78 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 24611. The strain has extremely strong inhibition capability on fusarium oxysporum which is pathogenic bacteria of watermelon fusarium wilt, has the functions of dissolving phosphorus, producing auxin fixedly, improving salt and alkali tolerance of plants and the like, and the bio-organic fertilizer prepared by the strain with good colonization capability on plant rhizosphere can obviously prevent watermelon fusarium wilt diseases, convert substances which are difficult to utilize in soil and promote plant growth and improve salt and alkali tolerance of plants.
Description
Technical Field
The invention belongs to the technical field of agricultural microbial agents, and relates to bacillus mojavensis YL-78 and application thereof.
Background
Land salinization is a serious problem affecting agricultural production and ecological environment, and along with global warming, the salinization degree of soil in arid and semiarid regions is aggravated, and the salinized soil area is continuously enlarged. The existing physical and chemical improvement mode is time-consuming, efficient and underground, has large investment, utilizes the interaction of microorganisms and plants, enhances the tolerance of the plants to the saline-alkali environment, enhances the stress resistance of the plants, and has the advantages of small investment, simple operation, quick response and the like. Therefore, the improvement of the utilization of the saline-alkali soil by the plants through microorganisms has important practical significance.
Some rhizosphere microorganisms can enhance the stress-resistant growth capability of plants, inhibit the growth and reproduction of harmful microorganisms, are harmless to plant bodies and natural environments, and have the functions of participating in the metabolic activity of the plants and improving the ecological environments, so that the rhizosphere microorganisms have good research and application values. Therefore, the microorganism for improving the saline-alkali tolerance of plants is separated from the saline-alkali soil, is used for improving the saline-alkali soil, increases the crop yield and has very important significance for improving the land utilization rate.
Disclosure of Invention
The invention aims to provide bacillus mojavensis YL-78 and application thereof, which are separated from the rhizosphere soil of continuous cropping corn in saline-alkali soil, are used for agricultural production, can inhibit fusarium oxysporum, promote plant growth and improve the saline-alkali tolerance of plants, and can be used for preparing biological organic fertilizers.
The technical scheme for solving the technical problems is as follows.
Bacillus mojavensis (Bacillus mojavensis) YL-78 is separated from the rhizosphere soil of continuous cropping corn in saline-alkali soil, is named YL-78 and is preserved in China general microbiological culture Collection center (CGMCC), and the preservation number is as follows: CGMCC No.24611, preservation address: beijing, chaoyang area, north Chenxi way No.1, 3, postal code 100101; preservation date: 2022, 3 and 30.
The bacillus mojavensis YL-78 is identified, and the thallus is characterized in that: the bacterial colony is round, the center is convex, the color is white, the edge is irregular, and the bacterial body is dry; the microscopic observation is characterized by a rod-shaped, single spore, positive gram staining and spore.
The Bacillus mojavensis YL-78 is cultured by using an autonomously selected culture medium formula, and the effective viable count of the obtained Bacillus mojavensis YL-78 is more than 5 multiplied by 10 9 CFU/mL。
The application of bacillus mojavensis YL-78 is used for preparing a microbial preparation for inhibiting bacteria.
Further, the germ is fusarium oxysporum which is a pathogenic bacterium of watermelon fusarium wilt.
The application of bacillus mojavensis YL-78 is used for preparing a microbial preparation for promoting plant growth.
Further, the method is used for preparing a microbial preparation for promoting the growth of cucumber seedlings.
The application of bacillus mojavensis YL-78 is used for preparing a saline-alkali soil conditioner; has the capability of strong phosphate dissolution, auxin production and improvement of saline-alkali tolerance of plants.
The application of bacillus mojavensis YL-78 is used for preparing the biological organic fertilizer; has good colonization ability to plant rhizosphere.
The bacillus mojavensis YL-78 and the application thereof have the beneficial effects that bacillus mojavensis is separated from the rhizosphere soil of the continuous cropping corn in the saline-alkali soil and named YL-78; the strain has the capability of inhibiting germs, in particular to fusarium oxysporum which is a pathogenic bacterium of watermelon fusarium wilt; the strain has the functions of strong phosphate dissolving, auxin fixation, improvement of plant saline-alkali tolerance and the like, and simultaneously has good colonization capacity on plant rhizosphere; the biological organic fertilizer prepared by the microbial inoculum can obviously prevent watermelon wilt and transform substances which are difficult to be utilized by plants in soil, promote plant growth and improve salt and alkali resistance of plants.
Drawings
FIG. 1 is a colony characterization of Bacillus mojavensis YL-78 of the present invention;
FIG. 2 shows the diameter of pathogenic bacteria colonies measured in the experiment for inhibiting pathogenic bacteria of example 2 of the present invention;
FIG. 3 is a band of bacteria measured in the experiment of inhibiting bacteria in example 2 of the present invention;
FIG. 4 is a photograph of inorganic phosphorus plate of example 4 of the present invention;
FIG. 5 is a graph showing the effect of example 4 of the present invention on the salt and alkali tolerance of sweet sorghum.
Detailed Description
Example 1
Isolation and identification of Bacillus mojavensis YL-78.
(1) Bacterial strain source: taking Ningxia Pingrow saline-alkali soil continuous cropping corn rhizosphere soil as test soil;
(2) The formula of the culture medium comprises: the composition of the culture medium formula adopting autonomous screening is as follows: feSO 4 ·7H 2 O0.15g，MgSO 4 ·7H 2 O 0.05g，MnSO 4 ·7H 2 O0.2 g, bean cake powder 0.5g, K 2 HPO 4 0.75g，CaCO 3 1.0g, 4g of peptone, 2g of yeast powder, 3.5g of sucrose, 5g of starch, 1L of distilled water, pH=7.0 and sterilizing at 121 ℃ for 30min; the fermentation conditions are as follows: 30 ℃ and rotating speed of 180rpm/min;
(3) Identification of strains: the bacterial colony is characterized in that the bacterial colony is round, the center is convex, the color is white, the edge is irregular, and the bacterial colony is dry; the shape of the microscopic observation body is characterized by a rod shape, a monospore, gram staining positive and spore; as shown in fig. 1;
(4) Effective viable count: the effective viable count of the strain is greater than 5×10 9 CFU/mL；
(5) Sequencing genes: extracting genome DNA of the strain, and identifying the strain by utilizing targeted DNA sequencing, wherein the gene sequence of the strain is shown as SEQ ID NO. 1; sequencing results show that the homology with Bacillus mojavensis (NR 024693.1) is 99.720% and the homology with Bacillus mojavensis (NR 112725.1) is 99.720% through blast comparison, so that the strain can be judged to be Bacillus mojavensis (Bacillus mojavensis) and named YL-78;
(6) Biological preservation: after identifying and confirming the species of the strain, performing biological preservation on the strain; preservation number: CGMCC No.24611; classification naming: bacillus mojavensis Bacillus mojavensis; preservation unit: china general microbiological culture Collection center (China Committee for culture Collection); preservation address: beijing, chaoyang area, north Chenxi way No.1, 3, postal code 100101; preservation date: 2022, 3 and 30.
Example 2
Bacillus mojavensis YL-78 inhibitory ability.
Through a plate antagonism test, fusarium oxysporum which is a pathogenic bacterium of watermelon fusarium wilt is placed in the center of a PDA culture medium plate, bacillus mojavensis (Bacillus mojavensis) YL-78 in the example 1 is inoculated at the positions 2cm apart from the two sides of the colony edge, and the culture is carried out in the dark at the temperature of 28 ℃ in an incubator for 5 times.
After 2-5 days, measuring the colony diameter of pathogenic bacteria and the zone of bacteria inhibition, and the results are shown in figure 2 and figure 3 respectively; the graph shows that the strain has obvious inhibition capability on pathogenic bacteria; meanwhile, through multiple tests, the average relative hypha bacteriostasis rate reaches 73.84 percent.
Therefore, the strain can be used for preparing microbial preparations for inhibiting bacteria, in particular fusarium oxysporum for inhibiting watermelon fusarium wilt pathogenic bacteria.
Example 3
Bacillus mojavensis YL-78 has the capability of colonizing and promoting growth in plant rhizosphere.
The strain was inoculated into an autonomously selected medium (FeSO 4 ·7H 2 O 0.15g，MgSO 4 ·7H 2 O 0.05g，MnSO 4 ·7H 2 O0.2 g, bean cake powder 0.5g, K 2 HPO 4 0.75g，CaCO 3 1.0g, 4g of peptone, 2g of yeast powder, 3.5g of sucrose, 5g of starch, 1L of distilled water with pH=7.0, sterilization at 121 ℃ for 30min, culturing at 30 ℃ for 48 hours at 180rpm/min, and diluting the prepared bacterial liquid to 1×10 when the spore rate reaches 95% -7 、1×10 -8 And 1X 10 -9 For use and are denoted by YL-78-1, YL-78-2, YL-78-3, respectively.
Cucumber seeds with consistent growth vigor after surface disinfection and germination acceleration are planted in a sterilization matrix, and then 1ml of diluted bacterial liquid is respectively inoculated on the cucumber seeds to develop experiments of the plant rhizosphere colonization capability and growth promotion effect of different concentrations of the strain. Wherein, the colonization condition of the root system of the cucumber is shown in table 1, and the growth promotion effect on the cucumber seedlings is shown in table 2.
TABLE 1 colonization ability of bacterial solutions with different concentrations at the rhizosphere of cucumber
| Hundreds of millions/g of active bacteria | For 1 day | For 8 days | For 15 days | 22 days | 29 days |
| YL-78-1 | 0.95±0.1 | 0.70±0.02 | 0.27±0.01 | 0.09±0.00 | 0.05±0.00 |
| YL-78-2 | 1.35±0.21 | 0.95±0.07 | 1.10±0.09 | 0.65±0.07 | 0.38±0.02 |
| YL-78-3 | 5.90±0.14 | 3.80±0.03 | 1.65±0.11 | 0.70±0.00 | 0.35±0.02 |
TABLE 2 action of bacterial liquids with different concentrations on cucumber seedling stage
| Treatment of | Plant height/cm | Stem thickness/mm |
| CK | 8.5±0.2c | 2.1±0.2b |
| YL-78-1 | 11.0±0.2b | 2.2±0.3ab |
| YL-78-2 | 12.6±0.6a | 2.3±0.1a |
| YL-78-3 | 11.9±0.4ab | 2.4±0.2a |
As shown in Table 1, the Bacillus mojavensis YL-78 was able to colonize the rhizosphere of cucumber for a long period of time, but the bacterial count was significantly reduced as time passed to 29 days. Wherein YL-78-1 has the lowest rhizosphere bacteria number at 29 days due to low inoculation amount, and YL-78-2 and YL-78-3 have close viable bacteria number at 29 days.
As is clear from Table 2, inoculating Bacillus mojavensis YL-78 significantly increased the plant height and stem thickness of cucumber seedlings as compared with CK without the addition of the microbial inoculum, wherein YL-78-2 had the best effect of promoting the plant height of cucumber seedlings, increased cucumber by 48.23%, followed by YL-78-3, by 40.0%.
Therefore, the bacillus mojavensis YL-78 can be used for preparing a microbial preparation for promoting plant growth, in particular to preparing a microbial preparation for promoting cucumber seedling growth.
Example 4
Application of Bacillus mojavensis YL-78 in saline-alkali soil is provided.
(1) Identification of the ability to secrete auxin
Independently sterilizing tryptophan and then adding the tryptophan into an LB liquid culture medium to ensure that the final concentration is 100mg/L; shake cultivation is carried out for 1 day after single colony inoculation respectively, the condition is 30 ℃, and the shaking speed is 180rpm; centrifuging 1mL of bacterial liquid at 10000rpm for 10min, dripping 100 mu L of supernatant onto a white drip plate, taking a blank culture medium and 50mg/L IAA solution as negative and positive controls respectively, adding an equivalent amount of Salkowski chromogenic liquid, and standing at room temperature in a dark place for 30min; mixing 1mL of supernatant with an equal volume of Salkowski chromogenic solution, placing the chromogenic solution in a water bath at 40 ℃ to react for 30min in a dark place, measuring absorbance at a wavelength of 530nm by a colorimetric method, and simultaneously measuring an OD600 value of the bacterial suspension; and calculating the IAA amount generated by the bacterial liquid per unit volume when the bacterial liquid concentration OD600 = 1 by combining with an IAA concentration standard curve.
As shown by the determination result of the auxin production capacity of the strain, the Bacillus mojavensis YL-78 has stronger auxin secretion capacity and reaches 8.21 mug/mL.
(2) Identification of the ability to dissolve inorganic phosphorus
Inoculating single colony to inorganic phosphorus culture medium, shake culturing at 30deg.C at 180rmp for two days; centrifuging the bacterial liquid at 10000rpm for 10min, collecting 5mL bacterial liquid supernatant, filtering with 0.22 μm filter membrane, and diluting; adding 5mL of diluent into a 50mL volumetric flask, adding 5mL of molybdenum-antimony anti-colorimetric solution, and adding deionized water to scale marks; after standing for 30min, measuring the absorbance OD880 at 880 nm; and drawing a phosphorus concentration standard curve, and calculating the phosphorus content dissolved out of the bacterial liquid according to the phosphorus standard curve and the OD880 value of the bacterial liquid.
As shown by the determination result of the phosphate-dissolving capability of the strain, the bacillus mojavensis YL-78 has stronger phosphate-dissolving capability, reaches 5.41 mug/mL, and the inorganic phosphate-dissolving flat plate photograph is shown in figure 4.
(3) Identification for improving saline-alkali tolerance of plants
Taking sweet sorghum as a plant to be tested, respectively setting a moderate saline-alkali contrast in a moderate saline-alkali environment with a salt concentration of 4.5 per mill and a pH value of 8.5, and improving saline-alkali tolerance microorganisms of the plant by moderate saline-alkali inoculation; by measuring growth indexes such as plant height, stem thickness, root length and the like of sweet sorghum seedlings, compared with a control group, the bacillus mojavensis YL-78 has remarkable saline-alkali resistance and growth promotion effects, wherein the plant height of the sweet sorghum seedlings is increased by 65%, the stem thickness is increased by 59%, and the root length is increased by 73%. The effect of Bacillus mojavensis YL-78 on the salt and alkali tolerance of sweet sorghum under moderate salt and alkali conditions is shown in figure 5.
Therefore, the bacillus mojavensis YL-78 has the functions of producing auxin (IAA) fixedly, dissolving phosphorus strongly and improving the saline-alkali tolerance of plants, and can be used for preparing saline-alkali soil conditioners.
Example 5
Bacillus mojavensis YL-78 is used for preparing the bio-organic fertilizer.
Taking chicken manure organic fertilizer as a nutrition carrier of bacillus mojavensis YL-78, inoculating the strain during secondary fermentation of the organic fertilizer, enabling the effective viable count of the strain to be not less than 0.5 hundred million/g at the end of fermentation, and periodically detecting the survival rate of the strain; the detection results of the effective viable count of the microorganisms for preparing the bio-organic fertilizer by the bacillus mojavensis YL-78 are shown in table 3.
TABLE 3 colonization ability of Bacillus mojavensis YL-78 in chicken manure bioorganic fertilizer
| For 1 day | For 7 days | For 15 days | For 30 days | For 60 days | 90 days | 120 days | 150 days | 180 days | |
| Viable count cfu/g | 2.23 | 2.05 | 1.84 | 1.53 | 1.05 | 1.12 | 1.05 | 0.85 | 0.54 |
As shown in Table 3, the bacillus mojavensis YL-78 can survive in the chicken manure bio-organic fertilizer for at least 180 days, has stronger biological activity, and can be used as an important strain of agricultural microorganism products; namely, the chicken manure bio-organic fertilizer prepared by the bacillus mojavensis YL-78 has stronger bioactivity stability.
Claims (9)
1. The bacillus mojavensis YL-78 is characterized by being preserved in China general microbiological culture Collection center with the preservation number of CGMCC No.24611.
2. The bacillus mojavensis YL-78 of claim 1, wherein the cells are characterized by: the bacterial colony is round, the center is convex, the color is white, the edge is irregular, and the bacterial body is dry; the microscopic observation is characterized by a rod-shaped, single spore, positive gram staining and spore.
3. The Bacillus mojavensis YL-78 of claim 1, wherein the effective viable count is > 5×10 9 CFU/mL。
4. Use of bacillus mojavensis YL-78 as claimed in any one of claims 1 to 3 for the preparation of a microbial preparation for the inhibition of pathogens.
5. The use of bacillus mojavensis YL-78 as claimed in claim 4, wherein the pathogen is fusarium oxysporum, a pathogenic bacterial fusarium oxysporum.
6. Use of bacillus mojavensis YL-78 as claimed in any one of claims 1 to 3 for the preparation of a microbial preparation for promoting plant growth.
7. The use of bacillus mojavensis YL-78 as claimed in claim 6 for the preparation of a microbial preparation for promoting the growth of cucumber seedlings.
8. Use of bacillus mojavensis YL-78 as claimed in any one of claims 1 to 3 for the preparation of a saline-alkali soil conditioner.
9. Use of bacillus mojavensis YL-78 as claimed in any one of claims 1 to 3 for the preparation of bio-organic fertilizers.
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