CN102755362B - Red ginseng processing method - Google Patents
Red ginseng processing method Download PDFInfo
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- CN102755362B CN102755362B CN201110109186.0A CN201110109186A CN102755362B CN 102755362 B CN102755362 B CN 102755362B CN 201110109186 A CN201110109186 A CN 201110109186A CN 102755362 B CN102755362 B CN 102755362B
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Abstract
The invention relates to a traditional Chinese medicine technology, in particular to a red ginseng processing method. The method comprises the steps of washing fresh ginseng, steaming for 4 hours, drying for 8 hours at 70 DEGC, and thoroughly drying at 50 DEG C, wherein the fresh ginseng is unprocessed ginseng.
Description
Technical field:
The present invention relates to a kind of traditional Chinese medicine technology, particularly a kind of red ginseng processing method.
Background technology:
Radix Ginseng is the dry root welding technology of Araliaceae Radix Ginseng Panax ginseng CA.Mey., is mainly distributed in three provinces in the northeast of China.Complex chemical composition in Radix Ginseng, but generally believe that principle active component is ginsenoside, account for 4%.General Radix Ginseng is used as medicine after concocting, and wherein, Radix Ginseng Rubra is modal processed product.Radix Ginseng Rubra after concocting, except easily preserving, also reduces the Radix Notoginseng cellulose content of neuron excitotoxicity, and defines new ginsenoside, as Rg3 and Rh2 of anti-tumor activity
[1 ~ 4].
Find in the Radix Ginseng resource survey process of northeast in the recent period, the red ginseng processing technique of red ginseng processing factory is different, certainly will cause the difference of Radix Ginseng Rubra inherent quality like this.What these processing factories focused on is the external quality of Radix Ginseng Rubra, does not pay close attention to the content of saponin component and saponins finger printing.So Radix Ginseng Rubra quality is uneven on market, simultaneously because brand is many and assorted, the in-fighting between enterprise causes Chinese red ginseng cannot contend with Radix Panacis Quinquefolii, Radix Ginseng so far.
In order to provide a unified quality standard to manufacturer, we are with reference to the technique of processing factory, first using Radix Ginseng total saponins and high activity ginsenoside Rg3 jointly as index, red ginseng processing technique is optimized.
Summary of the invention:
The present invention obtains a kind of excellent red ginseng processing technology through screening, and the invention provides a kind of red ginseng processing method, the method comprises for this reason, and fresh ginseng steams 4h, dries 8h at 70 DEG C, then thoroughly dry at 50 DEG C after cleaning.Wherein said fresh ginseng is the Radix Ginseng without processing.Described clean employing method for washing is cleaned.Described steaming adopts steamer to steam.Described oven dry adopts baking box to dry.
Method of the present invention, be through screening and obtain, screening process is as follows:
1 material and instrument
The fresh Radix Ginseng of 1.1 material 5 years, prosperous safe Pharmaceutical provides, and through Tianjin TianShiLi ZhiJiao Medicine Co., Ltd Yang Yuewu researcher qualification, is Radix Ginseng Panax ginseng CA.Mey.
1.2 reference substance ginsenoside Res, Rg3 are that Nat'l Pharmaceutical & Biological Products Control Institute buys (lot number is 110754-200822,110804-200402).
1.3 instrument Waters 2695 high performance liquid chromatographs, 2489 UV-detector; Hach DR5000 ultraviolet spectrophotometer; DIKMAProElut
tMsPE pillar.
2 experimental techniques
2.1 red ginseng processing Orthogonal Experiment and Design adopt positive quadraturing design test, and carry out condition preferably to Radix Ginseng Rubra steaming time, bake out temperature, drying time, inspection target is the content of Radix Ginseng total saponins and activated saponin Rg3.By L9 (3,4) orthogonal table experiment arrangement.Factor level table is in table 1.
Table 1 factor level table
Steaming time was divided into for three stages, 50 minute temperature rise period, and 30 minutes temperature-fall periods, and three phases-time summations are test steaming time in orthogonal table.Test drying course takes secondary drying method, and first time dries and carries out according to orthogonal test table, second time be the baking oven that the Radix Ginseng unification that first time has dried puts into 50 DEG C be dried to completely dry till.Experiment is total to obtain nine kinds of Radix Ginseng Rubra samples.
The mensuration of 2.2 total Ginsenosides Contents
2.2.1 Radix Ginseng Rubra 100g (Radix Ginseng Rubra crude drug removing LU) is got in the preparation of need testing solution, pulverizes No. 4 sieves, and mixing, gets 5g powder, accurately weighed, puts in round-bottomed flask, adds chloroform 50ml reflux 3h, discard chloroform solution; Then, after medicinal residues volatilize solvent, add 85% alcohol reflux 2 times, each addition is 40ml, and return time is respectively 2h and 1h.Extracting solution decompression recycling ethanol is to without alcohol taste, and the methanol solution with 20% of 0.5mol/L sodium hydroxide shifts and is settled in 50ml measuring bottle.Precision measures above-mentioned solution 10ml, the DIKMAProElut above anticipated
tMsPE pillar (first uses about 10ml washed with methanol, be about 5ml with 20% methanol solution of the sodium hydroxide of 0.5mol/L again and rinse process), rinse with 20% methanol solution 5ml of the sodium hydroxide of 0.5mol/L, effluent discards, then rinses with 20% methanol solution 10ml, effluent discards, finally to be collected in 10ml measuring bottle to nearly scale by methanol-eluted fractions, to add methanol to scale, shake up, cross 0.45 μm of filter membrane, get subsequent filtrate as need testing solution.
2.2.2 reference substance solution is prepared precision and is taken ginsenoside Re's reference substance, adds methanol and makes the solution of every 1ml containing 2mg, shake up, to obtain final product.
2.2.3 measure precision and measure reference substance solution 20,40,60,80,100 μ l and need testing solution 40 μ l, put in 10ml tool plug test tube respectively.Put in water-bath after waving most solvent and take out, let cool, precision adds the 5% vanillin-glacial acetic acid of new preparation and perchloric acid mixes liquid (2: 8) 1ml, shakes up.Put in 60 DEG C of water-baths and heat 15min, take out, put in ice bath immediately and cool 2min.Precision adds glacial acetic acid 5ml, shakes up, and at room temperature places 5min.Take corresponding reagent as blank, measure trap at 550nm place, calculate, to obtain final product.
The mensuration of 2.3 ginsenoside Rg3's content
2.3.1 the same 2.2.1 of the preparation of need testing solution.
2.3.2 reference substance solution is prepared precision and is taken ginsenoside Rg3's reference substance, adds methanol and makes the solution of every 1ml containing 0.400mg, shake up, to obtain final product.
2.3.3 chromatographic condition Diamonsil TM C18 (250 × 4.6mm, 5 μm) chromatographic column; Take acetonitrile as mobile phase A, with 0.05% phosphoric acid solution for Mobile phase B, carry out gradient elution by table 2; Determined wavelength is 203nm; Column temperature is 30 DEG C; Flow velocity 1.0ml/min; Limit of integration 8 ~ 70min.Get reference substance solution and each 10 μ L of need testing solution respectively, injecting chromatograph, measure.Under this chromatographic condition, Rg3 is separated preferably.
Table 2 gradient elution program
3 experimental results
The orthogonal experiments of 3.1 Radix Ginseng total saponinss is in table 3.
Table 3 orthogonal experiments---Radix Ginseng total saponins
The analysis of variance table of 3.2 ginsenoside Rg3s is in table 4.
Table 4 the results of analysis of variance---Radix Ginseng total saponins
Note: F
0.01(2,2)=99.0, F
0.10(2,2)=9.0
The orthogonal experiments of 3.3 ginsenoside Rg3s is in table 5.
Table 5 orthogonal experiments---ginsenoside Rg3
The results of analysis of variance of 3.4 ginsenoside Rg3s is in table 6.
Table 6 the results of analysis of variance---ginsenoside Rg3
3 analysis and thinking
3.1 by table 3 and table 4, factor A has pole significance impact (P < 0.01) to Radix Ginseng total saponins, factor C has appreciable impact (P < 0.10), and the primary-slave relation of each Factors on Human ginseng total saponin content impact is A > C > B.From average, each level order of factor A and C is as K
2> K
1> K
3, illustrate in 3 levels, A
2c
2, namely steam 4h, oven dry 10h total saponin content is the highest.
3.2 by table 5 and table 6, and factor B and C has a significant impact (P < 0.10) ginsenoside Rg3's content.The primary-slave relation of each Factors on Human ginseng saponin Rg3 content impact is C > B > A.From average, each level order of factor C is K
1> K
2> K
3, each level order of factor B is K
3> K
2> K
1, illustrate in 3 levels, B
3c
1, namely 70 DEG C of oven dry, 8 hours Rg3 content is the highest.
3.3 content considering Radix Ginseng total saponins and Rg3, the optimum processing scheme that we finally select is A
2b
3c
1, after namely fresh ginseng is cleaned, steam 4h, at 70 DEG C, dry 8h, then thoroughly dry at 50 DEG C.Three batches of demonstration test results show that this process stabilizing is feasible.
Process of the present invention is effective, and technique is simple, is easy to promote.
Detailed description of the invention:
By the following examples, further illustrate the present invention, but not as limitation of the present invention.
Embodiment 1
Fresh ginseng steams 4h, dries 8h at 70 DEG C, then thoroughly dry at 50 DEG C after cleaning.
Claims (5)
1. a red ginseng processing method, is characterized in that, comprises the following steps: fresh ginseng steams 4h after cleaning, dry 8h at 70 DEG C, then thoroughly dry at 50 DEG C, described steaming time was divided into for three stages, 50 minute temperature rise period, and 30 minutes temperature-fall periods, three phases-time summations are 4h.
2. method according to claim 1, is characterized in that, described fresh ginseng is the Radix Ginseng without processing.
3. method according to claim 1, is characterized in that, described clean employing method for washing is cleaned.
4. method according to claim 1, is characterized in that, described in steam adopt steamer steam.
5. method according to claim 1, is characterized in that, described oven dry adopts baking box to dry.
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| CN103211846A (en) * | 2013-05-03 | 2013-07-24 | 南京农业大学 | Processing method of red ginseng |
| CN105362241B (en) * | 2015-12-04 | 2018-06-22 | 吉林人参研究院 | A kind of preparation method of refined ginseng tablet |
| CN105641009A (en) * | 2016-02-18 | 2016-06-08 | 吉林加健康产业股份有限公司 | Processing method of red ginseng |
| CN108142924A (en) * | 2016-12-06 | 2018-06-12 | 张成中 | The preparation and its application of purplish red ginseng |
| CN109010402A (en) * | 2018-09-11 | 2018-12-18 | 吉林人参研究院（吉林省长白山天然药物研究院） | A method of using fresh ginseng as the black ginseng of Raw material processing |
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| CN101244103A (en) * | 2008-03-24 | 2008-08-20 | 中国科学院长春应用化学研究所 | Method for processing Chinese medicine red ginseng |
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| CN101244103A (en) * | 2008-03-24 | 2008-08-20 | 中国科学院长春应用化学研究所 | Method for processing Chinese medicine red ginseng |
Non-Patent Citations (1)
| Title |
|---|
| 浅谈红参的加工技术;蔡荣春等;《特产研究》;19840331(第3期);第42页"二 红参的加工方法"项下，第41页右栏11-12行 * |
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