CN102747118B - Method for directly producing feeding isomaltooligosaccharide syrup by using wheat - Google Patents
Method for directly producing feeding isomaltooligosaccharide syrup by using wheat Download PDFInfo
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- CN102747118B CN102747118B CN201210199831.7A CN201210199831A CN102747118B CN 102747118 B CN102747118 B CN 102747118B CN 201210199831 A CN201210199831 A CN 201210199831A CN 102747118 B CN102747118 B CN 102747118B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for directly producing feeding isomaltooligosaccharide syrup by using wheat, comprising the following steps: (1) producing wheat malts and preparing into malt whey; (2) carrying out saccharification on the malt whey and preparing into malt converted mash; (3) carrying out press filtration on the malt converted mash and preparing into a converted mash filtrate; (4) boiling and precipitating the converted mash filtrate and preparing into a maltose solution; (5) carrying out transglycosidation on the maltose solution and preparing into an isomaltose hypgather liquid; and (6) concentrating the isomaltose hypgather liquid and preparing into a finished product. According to the invention, the isomaltooligosaccharide syrup can be obtained, and simultaneously byproduct malt spent grains can be obtained, thus value increment of wheat can be realized, and the problem that milk lacks green feed additives. The method has the advantages of simple process, short process flow, and low production cost, and is suitable for the special requirements of forage industry for product cost.
Description
Technical field:
The present invention relates to a kind of production method of utilizing the feeding oligomeric isomaltose slurry of wheat direct production, belong to biological enzyme manufacture and field of feed processing.
Background technology:
The Hetao area of Inner Mongolia Autonomous Region is one of China's spring wheat main producing region, and sown area is more than 100,000 hectares, and annual production is more than 700,000,000 kilograms, and wheat resource is abundant, need to carry out the additional increment of intensive processing and height and transform; Meanwhile, Inner Mongolia Autonomous Region has more than 200 ten thousand of milk cows, and cow feed additive market, after forbidding microbiotic, lacks green substitute--oligomeric isomaltose product, and breach amount reaches more than 100 tons/year; Also lack the green roughage that can increase milk production--Fructus Hordei Germinatus grain, more than breach amount reaches 1,000,000 tons/year (dry weights) simultaneously.The visible industry that wheat is converted into feeding oligomeric isomaltose additive and byproduct Fructus Hordei Germinatus grain, has obvious economic advantages in locality.
The oligomeric isomaltose production technology that Present Domestic is general is all to make starting raw material with starch.Starch is processed through gelatinization and liquefaction, then adds external source amylase, and saccharification is maltose; Maltose adds transglucosidase catalysis again, produces oligomeric isomaltose.The shortcoming of current this general technological method is: (1) raw materials used starch cost is high; (2) investment of production equipment is large.Its product is only applicable to field of food and uses, and while using as fodder additives, does not calculate, and is difficult to promote in aquaculture.So find a kind of be applicable to field of fodder, cheaply, easy new production process, be the specific demand in fodder industry field.
Summary of the invention:
The object of the present invention is to provide a kind of production method field of fodder, that utilize cheaply, easily the feeding oligomeric isomaltose slurry of wheat direct production that is applicable to.
Object of the present invention is implemented by following technical scheme: a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, and it includes following steps: the production of (1) wheat malt is also made Fructus Hordei Germinatus serum; (2) saccharification of Fructus Hordei Germinatus serum make malt mash; (3) press filtration of malt mash make converted mash filtrate; (4) coctoprecipitinogen of converted mash filtrate make maltose solution; (5) maltose solution turns glycosides and makes oligomeric isomaltose liquid; (6) oligomeric isomaltose liquid concentrated and and make finished product.
Described a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, the production of described step (1) wheat malt is also made Fructus Hordei Germinatus serum, include following steps: the choice criteria of a. wheat: selected wheat is except meeting the GB135186 regulation of brewageing wheat, also should meet following basic demand: moisture≤13%, percentage of germination >=90%, thousand seed weight >=35 gram, starch 57～64%, protein≤13%, germinating power >=85%, extraction rate >=82%, fat 1.5～23%;
B. wheat germination: get selected wheat by weight 1 times of above water soaking wheat 5～10 hours, after draining flowing water, put into germinating box, regulate the temperature inside the box to be retained to 15～20 ℃, spray water every day 2 times, germinate and carry out 2～4 days, temperature is risen to 18 ℃, continuing germinates again carries out 1～2 day, when now bud is grown average 3～5mm, stop germinateing, Fructus Hordei Germinatus outlet;
C. take off and must get grain: the wet Fructus Hordei Germinatus after outlet is put into propeller type mixer and stir 10～30 minutes, wheat root coring is fractureed and come off, sieve and remove cast, retain Fructus Hordei Germinatus grain standby;
D. colloidal mill grinds emulsification malt serum: above-mentioned Fructus Hordei Germinatus grain is put into colloidal mill, add the water of 1～1.2 times of amount of weight ratio, be milled to milk sap, make Fructus Hordei Germinatus serum.
Described a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, the saccharification of described step (2) Fructus Hordei Germinatus serum is also made malt mash, it includes following steps: by the Fructus Hordei Germinatus serum making, according to following condition, process saccharification: saccharification temperature is at 62 ℃～68 ℃, Ph value is adjusted to 6.0～6.5, saccharification time 1～2 hour, after be warming up to 78 ℃～80 ℃ of temperature; Iodine colour response index and the DE value of test saccharified liquid are the mass percent that reducing sugar accounts for syrup dry-matter, and question response carries out one section when not presenting iodine colour response again, until DE value is raised to 15～18, stops saccharification, and malt mash is moved in filter vat.In saccharifying, after crude amylase germinates Fructus Hordei Germinatus, remaining starch degradation is glucose and Oligomeric maltose (mainly comprising maltose, trisaccharide maltose, maltotetrose and above oligose); Proteolytic enzyme and lipase, make remaining protein and insoluble lipid material in Fructus Hordei Germinatus be degraded to soluble substance, finally forms malt mash.
Described a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, the press filtration of described step (3) malt mash is also made converted mash filtrate: it includes following steps:
A. pressure filtration: above-mentioned malt mash is moved in Fructus Hordei Germinatus grain filter pocket in the lump, start filter vat suction pump, carry out pressure filtration, 78 ℃～80 ℃ of washing trough water temps, Ph value 6.0～6.5, filters after 25～30min, start cultivated scouring machine and stir filter residue, add and wash poor water, again filter, the consumption of washing poor water is 5～10% of total inventory by mass percentage; Making to filter rear index reaches: filtrate dry-matter accounts for the more than 80% of total material dry-matter, filter residue water ratio 65%～75%, filtrate water ratio 75%～65%;
B. obtaining of converted mash filtrate: after press filtration, isolated filter residue is Fructus Hordei Germinatus grain, moves in Fructus Hordei Germinatus grain storage tank, after transport diary farm to and slightly raise; After press filtration, isolated filtrate is converted mash filtrate.Wherein, Fructus Hordei Germinatus grain dry-matter output weight accounts for and drops into 15～25% of Fructus Hordei Germinatus grain dry weight.In isolated converted mash filtrate, main dry-matter composition is dextrin, oligosaccharides, glucose, still has in addition a small amount of starch, protein, lipid material, solvable nitrogenous thing, amylan thing, mineral substance etc.Now, the quality percentage composition of converted mash filtrate dry-matter is 25～35%; Wherein solvable glucide accounts for 15～25%.
Described a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, the coctoprecipitinogen of described step (4) converted mash filtrate is also made maltose solution:
A. boiling and precipitation of converted mash filtrate: converted mash filtrate is proceeded in heating kettle, and be heated to 100 ℃, continue more than 15～30 minutes, until water transpiration 10～20% left and right, then be cooled to 15 ℃ of room temperatures, standing to being precipitated to flocculation sediment in bottom completely, it is clear that liquid becomes;
B. the extraction of maltose solution: after the solid substance that flocculates is deposited in described heating kettle bottom, release out supernatant liquor to retort, obtain maltose solution.The flocculation solid quality that this process is removed accounts for 5～15% of maltose solution total mass; The dry biomass percentage composition of maltose solution is increased to 30～40% because of concentrated.
Described a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, the turning glycosides and make oligomeric isomaltose liquid of described step (5) maltose solution:
A. the modulation of substrate solution: the maltose solution being generated by step (4), the Oligomeric maltose 30～40% that contains mass percent, regulates ph to 5.0～5.5 of described maltose solution with acetic acid, and is heated to 55 ℃～60 ℃;
B. the selection of zymin and consumption: a-glucuroide and aspergillus oryzae a-amylase are dropped in described maltose solution, stir evenly, wherein the addition of a-glucuroide is 0.5～1.5TGU/g dry-matter; The diastatic addition of aspergillus oryzae a-is 1～5SKUB/g dry-matter.In the maltose solution that step (4) generates, the killing of the deactivation of enzyme, mushroom, the flocculation removal of protein macromolecule and the clarification of solution, for this enzymatic reaction provides condition.
C. reaction times and product monitoring: the glycosides that turns that continues above-mentioned steps a and b reacts 20～28h, after abstraction reaction liquid test passes, is warming up to 80 ℃ of inactivators, stopped reaction.The reaction solution extracting need be tested isomaltose (IG2), panose (P), Isomaltotriose (IG3), tetrose (Gn) content wherein.The total mass of oligomeric isomaltose in reaction solution (IG2+P+IG3+Gn) accounts for ratio >=50% of total reducing sugar butt quality, or be greater than total solution quality 17.5% time, stopped reaction.
Described a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, described step (6) oligomeric isomaltose liquid concentrated and and make finished product.
A. evaporation concentration: temperature of reaction is raised to 100 ℃, evaporates the moisture of the mass percent 10～20% of total moisture content, make dry-matter be concentrated into mass percent 50～55%, the oligomeric isomaltose syrup that product becomes thick pulpous state is product;
B. the packing of product: be filled into sealing in 200L regular barrel, be finished product.
The invention has the advantages that: (1) oligomeric isomaltose is a kind of green feed additive, and animal is had to nourishing function, makes an addition in feed and can reduce antibiotic use; Fructus Hordei Germinatus grain is the newborn feed of a kind of increasing of milk cow, the present invention utilizes the production method of the feeding oligomeric isomaltose slurry of Wheat Production not only can make oligomeric isomaltose slurry, simultaneously, can also obtain byproduct-Fructus Hordei Germinatus grain, Fructus Hordei Germinatus grain is the green roughage of fodder for increasing milk that Vehicles Collected from Market is in short supply, increased productivity effect, this conversion can make wheat obviously rise in value, and solves the problem that milk cow lacks green feed additive simultaneously; (2) the present invention utilizes wheat seed nature germination process to replace external source amylase starch saccharification process, greatly reduces production costs; (3) by wort filtration and precipitate and separate technology generations, replaced the production process of full Wheat Production starch, reduced process procedure, reduced cost, production unit is simple, greatly reduces facility investment; (4) technique of the present invention is simple, and technical process is short, with the general isomaltose production technique comparison of current field of food, saves diastatic use, has obviously reduced production cost, is applicable to the particular requirement of feedstuff industry to product cost.
Embodiment:
Embodiment 1: a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, and it includes following steps: the production of (1) wheat malt is also made Fructus Hordei Germinatus serum; (2) saccharification of Fructus Hordei Germinatus serum make malt mash; (3) press filtration of malt mash make converted mash filtrate; (4) coctoprecipitinogen of converted mash filtrate make maltose solution; (5) maltose solution turns glycosides and makes oligomeric isomaltose liquid; (6) oligomeric isomaltose liquid concentrated and and make finished product.
Wherein, the production of step (1) wheat malt is also made Fructus Hordei Germinatus serum, include following steps: the choice criteria of a. wheat: selected wheat, except meeting the GB135186 regulation of brewageing wheat, also should meet following basic demand: moisture≤13%, percentage of germination >=90%, thousand seed weight >=35 gram, starch 57～64%, protein≤13%, germinating power >=85%, extraction rate >=82%, fat 1.5～23%;
B. wheat germination: get selected wheat by weight 1 times of above water soaking wheat 5 hours, after draining flowing water, put into germinating box, regulate the temperature inside the box to be retained to 15 ℃, spray water every day 2 times, germinate and carry out 2 days, temperature is risen to 18 ℃, continuing germinates again carries out 1 day, when now bud is grown average 3mm, stop germinateing, Fructus Hordei Germinatus outlet;
C. take off and must get grain: the wet Fructus Hordei Germinatus after outlet is put into propeller type mixer and stir 10 minutes, wheat root coring is fractureed and come off, sieve and remove cast, retain Fructus Hordei Germinatus grain standby;
D. colloidal mill grinds emulsification malt serum: above-mentioned Fructus Hordei Germinatus grain is put into colloidal mill, add the water of 1 times of amount of weight ratio, be milled to milk sap, make Fructus Hordei Germinatus serum.
The saccharification of step (2) Fructus Hordei Germinatus serum is also made malt mash, it includes following steps: by the Fructus Hordei Germinatus serum making, according to following condition, process saccharification: saccharification temperature is at 62 ℃, and Ph value is adjusted to 6.0, saccharification time 1 hour, after be warming up to 78 ℃ of temperature; Iodine colour response index and the DE value of test saccharified liquid are the mass percent that reducing sugar accounts for syrup dry-matter, and question response carries out one section when not presenting iodine colour response again, until DE value is raised to 15, stops saccharification, and malt mash is moved in filter vat.In saccharifying, after crude amylase germinates Fructus Hordei Germinatus, remaining starch degradation is glucose and Oligomeric maltose (mainly comprising maltose, trisaccharide maltose, maltotetrose and above oligose); Proteolytic enzyme and lipase, make remaining protein and insoluble lipid material in Fructus Hordei Germinatus be degraded to soluble substance, finally forms malt mash.
The press filtration of step (3) malt mash is also made converted mash filtrate: it includes following steps:
A. pressure filtration: above-mentioned malt mash is moved in Fructus Hordei Germinatus grain filter pocket in the lump, start filter vat suction pump, carry out pressure filtration, 78 ℃ of washing trough water temps, Ph value 6.0, filters after 25min, start cultivated scouring machine and stir filter residue, add and wash poor water, again filter, the consumption of washing poor water is 5% of total inventory by mass percentage; Making to filter rear index reaches: filtrate dry-matter accounts for the more than 80% of total material dry-matter, filter residue water ratio 65%～75%, filtrate water ratio 75%～65%;
B. obtaining of converted mash filtrate: after press filtration, isolated filter residue is Fructus Hordei Germinatus grain, moves in Fructus Hordei Germinatus grain storage tank, after transport diary farm to and slightly raise; After press filtration, isolated filtrate is converted mash filtrate.Wherein, Fructus Hordei Germinatus grain dry-matter output weight accounts for and drops into 16% of Fructus Hordei Germinatus grain dry weight.In isolated converted mash filtrate, main dry-matter composition is dextrin, oligosaccharides, glucose, still has in addition a small amount of starch, protein, lipid material, solvable nitrogenous thing, amylan thing, mineral substance etc.Now, the quality percentage composition of converted mash filtrate dry-matter is 25%; Wherein solvable glucide accounts for 17%.
The coctoprecipitinogen of step (4) converted mash filtrate is also made maltose solution:
A. boiling and precipitation of converted mash filtrate: converted mash filtrate is proceeded in heating kettle, and be heated to 100 ℃, continue more than 15 minutes, until water transpiration 10% left and right, then be cooled to 15 ℃ of room temperatures, standing to being precipitated to flocculation sediment in bottom completely, it is clear that liquid becomes;
B. the extraction of maltose solution: after the solid substance that flocculates is deposited in described heating kettle bottom, release out supernatant liquor to retort, obtain maltose solution.The flocculation solid quality that this process is removed accounts for 5% of maltose solution total mass; The dry biomass percentage composition of maltose solution is increased to 30% because of concentrated.
The turning glycosides and make oligomeric isomaltose liquid of step (5) maltose solution:
A. the modulation of substrate solution: the maltose solution being generated by step (4), the Oligomeric maltose 30% that contains mass percent, regulates the ph to 5.0 of described maltose solution with acetic acid, and is heated to 55 ℃;
B. the selection of zymin and consumption: a-glucuroide and aspergillus oryzae a-amylase are dropped in maltose solution, stir evenly, wherein the addition of a-glucuroide is 0.5TGU/g dry-matter; The diastatic addition of aspergillus oryzae a-is 1SKUB/g dry-matter.In the maltose solution that step (4) generates, the killing of the deactivation of enzyme, mushroom, the flocculation removal of protein macromolecule and the clarification of solution, for this enzymatic reaction provides condition.
C. reaction times and product monitoring: the glycosides that turns that continues above-mentioned steps a and b reacts 20h, after abstraction reaction liquid test passes, is warming up to 80 ℃ of inactivators, stopped reaction.The reaction solution extracting need be tested isomaltose (IG2), panose (P), Isomaltotriose (IG3), tetrose (Gn) content wherein.The total mass of oligomeric isomaltose in reaction solution (IG2+P+IG3+Gn) accounts for ratio >=50% of total reducing sugar butt quality, or be greater than total solution quality 17.5% time, stopped reaction.
Step (6) oligomeric isomaltose liquid concentrated and and make finished product.
A. evaporation concentration: temperature of reaction is raised to 100 ℃, evaporates the moisture of the mass percent 10% of total moisture content, make dry-matter be concentrated into mass percent 50%, the oligomeric isomaltose syrup that product becomes thick pulpous state is product;
B. the packing of product: be filled into sealing in 200L regular barrel, be finished product.
Embodiment 2: a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, and it includes following steps: the production of (1) wheat malt is also made Fructus Hordei Germinatus serum; (2) saccharification of Fructus Hordei Germinatus serum make malt mash; (3) press filtration of malt mash make converted mash filtrate; (4) coctoprecipitinogen of converted mash filtrate make maltose solution; (5) maltose solution turns glycosides and makes oligomeric isomaltose liquid; (6) oligomeric isomaltose liquid concentrated and and make finished product.
Wherein, the production of step (1) wheat malt is also made Fructus Hordei Germinatus serum, include following steps: the choice criteria of a. wheat: selected wheat, except meeting the GB135186 regulation of brewageing wheat, also should meet following basic demand: moisture≤13%, percentage of germination >=90%, thousand seed weight >=35 gram, starch 57～64%, protein≤13%, germinating power >=85%, extraction rate >=82%, fat 1.5～23%;
B. wheat germination: get selected wheat by weight 1 times of above water soaking wheat 10 hours, after draining flowing water, put into germinating box, regulate the temperature inside the box to be retained to 20 ℃, spray water every day 2 times, germinate and carry out 4 days, temperature is risen to 18 ℃, continuing germinates again carries out 2 days, when now bud is grown average 5mm, stop germinateing, Fructus Hordei Germinatus outlet;
C. take off and must get grain: the wet Fructus Hordei Germinatus after outlet is put into propeller type mixer and stir 30 minutes, wheat root coring is fractureed and come off, sieve and remove cast, retain Fructus Hordei Germinatus grain standby;
D. colloidal mill grinds emulsification malt serum: above-mentioned Fructus Hordei Germinatus grain is put into colloidal mill, add the water of 1～1.2 times of amount of weight ratio, be milled to milk sap, make Fructus Hordei Germinatus serum.
The saccharification of step (2) Fructus Hordei Germinatus serum is also made malt mash, it includes following steps: by the Fructus Hordei Germinatus serum making, according to following condition, process saccharification: saccharification temperature is at 68 ℃, and Ph value is adjusted to 6.5, saccharification time 2 hours, after be warming up to 80 ℃ of temperature; Iodine colour response index and the DE value of test saccharified liquid are the mass percent that reducing sugar accounts for syrup dry-matter, and question response carries out one section when not presenting iodine colour response again, until DE value is raised to 18, stops saccharification, and malt mash is moved in filter vat.In saccharifying, after crude amylase germinates Fructus Hordei Germinatus, remaining starch degradation is glucose and Oligomeric maltose (mainly comprising maltose, trisaccharide maltose, maltotetrose and above oligose); Proteolytic enzyme and lipase, make remaining protein and insoluble lipid material in Fructus Hordei Germinatus be degraded to soluble substance, finally forms malt mash.
The press filtration of step (3) malt mash is also made converted mash filtrate: it includes following steps:
A. pressure filtration: above-mentioned malt mash is moved in Fructus Hordei Germinatus grain filter pocket in the lump, start filter vat suction pump, carry out pressure filtration, 80 ℃ of washing trough water temps, Ph value 6.5, after filter 23 0min, start cultivated scouring machine and stir filter residue, add and wash poor water, again filter, the consumption of washing poor water is 10% of total inventory by mass percentage; Making to filter rear index reaches: filtrate dry-matter accounts for the more than 80% of total material dry-matter, filter residue water ratio 65%～75%, filtrate water ratio 75%～65%;
B. obtaining of converted mash filtrate: after press filtration, isolated filter residue is Fructus Hordei Germinatus grain, moves in Fructus Hordei Germinatus grain storage tank, after transport diary farm to and slightly raise; After press filtration, isolated filtrate is converted mash filtrate.Wherein, Fructus Hordei Germinatus grain dry-matter output weight accounts for and drops into 25% of Fructus Hordei Germinatus grain dry weight.In isolated converted mash filtrate, main dry-matter composition is dextrin, oligosaccharides, glucose, still has in addition a small amount of starch, protein, lipid material, solvable nitrogenous thing, amylan thing, mineral substance etc.Now, the quality percentage composition of converted mash filtrate dry-matter is 35%; Wherein solvable glucide accounts for 25%.
The coctoprecipitinogen of step (4) converted mash filtrate is also made maltose solution:
A. boiling and precipitation of converted mash filtrate: converted mash filtrate is proceeded in heating kettle, and be heated to 100 ℃, continue more than 30 minutes, until water transpiration 20% left and right, then be cooled to 15 ℃ of room temperatures, standing to being precipitated to flocculation sediment in bottom completely, it is clear that liquid becomes;
B. the extraction of maltose solution: after the solid substance that flocculates is deposited in described heating kettle bottom, release out supernatant liquor to retort, obtain maltose solution.The flocculation solid quality that this process is removed accounts for 15% of maltose solution total mass; The dry biomass percentage composition of maltose solution is increased to 40% because of concentrated.
The turning glycosides and make oligomeric isomaltose liquid of step (5) maltose solution:
A. the modulation of substrate solution: the maltose solution being generated by step (4), the Oligomeric maltose 40% that contains mass percent, regulates the ph to 5.5 of described maltose solution with acetic acid, and is heated to 60 ℃;
B. the selection of zymin and consumption: a-glucuroide and aspergillus oryzae a-amylase are dropped in described maltose solution, stir evenly, wherein the addition of a-glucuroide is 1.5TGU/g dry-matter; The diastatic addition of aspergillus oryzae a-is 5SKUB/g dry-matter.In the maltose solution that step (4) generates, the killing of the deactivation of enzyme, mushroom, the flocculation removal of protein macromolecule and the clarification of solution, for this enzymatic reaction provides condition.
C. reaction times and product monitoring: the glycosides that turns that continues above-mentioned steps a and b reacts 28h, after abstraction reaction liquid test passes, is warming up to 80 ℃ of inactivators, stopped reaction.The reaction solution extracting need be tested isomaltose (IG2), panose (P), Isomaltotriose (IG3), tetrose (Gn) content wherein.The total mass of oligomeric isomaltose in reaction solution (IG2+P+IG3+Gn) accounts for ratio >=50% of total reducing sugar butt quality, or be greater than total solution quality 17.5% time, stopped reaction.
Step (6) oligomeric isomaltose liquid concentrated and and make finished product.
A. evaporation concentration: temperature of reaction is raised to 100 ℃, evaporates the moisture of the mass percent 20% of total moisture content, make dry-matter be concentrated into mass percent 55%, the oligomeric isomaltose syrup that product becomes thick pulpous state is product;
B. the packing of product: be filled into sealing in 200L regular barrel, be finished product.
Embodiment 3: a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, and it includes following steps: the production of (1) wheat malt is also made Fructus Hordei Germinatus serum; (2) saccharification of Fructus Hordei Germinatus serum make malt mash; (3) press filtration of malt mash make converted mash filtrate; (4) coctoprecipitinogen of converted mash filtrate make maltose solution; (5) maltose solution turns glycosides and makes oligomeric isomaltose liquid; (6) oligomeric isomaltose liquid concentrated and and make finished product.
Wherein, the production of step (1) wheat malt is also made Fructus Hordei Germinatus serum, include following steps: the choice criteria of a. wheat: selected wheat, except meeting the GB135186 regulation of brewageing wheat, also should meet following basic demand: moisture≤13%, percentage of germination >=90%, thousand seed weight >=35 gram, starch 57～64%, protein≤13%, germinating power >=85%, extraction rate >=82%, fat 1.5～23%;
B. wheat germination: get selected wheat by weight 1 times of above water soaking wheat 7 hours, after draining flowing water, put into germinating box, regulate the temperature inside the box to be retained to 18 ℃, spray water every day 2 times, germinate and carry out 3 days, temperature is risen to 18 ℃, continuing germinates again carries out 1 day, when now bud is grown average 4mm, stop germinateing, Fructus Hordei Germinatus outlet;
C. take off and must get grain: the wet Fructus Hordei Germinatus after outlet is put into propeller type mixer and stir 20 minutes, wheat root coring is fractureed and come off, sieve and remove cast, retain Fructus Hordei Germinatus grain standby;
D. colloidal mill grinds emulsification malt serum: above-mentioned Fructus Hordei Germinatus grain is put into colloidal mill, add the water of 1.1 times of amounts of weight ratio, be milled to milk sap, make Fructus Hordei Germinatus serum.
The saccharification of step (2) Fructus Hordei Germinatus serum is also made malt mash, it includes following steps: by the Fructus Hordei Germinatus serum making, according to following condition, process saccharification: saccharification temperature is at 65 ℃, and Ph value is adjusted to 6.2, saccharification time 1 hour, after be warming up to 79 ℃ of temperature; Iodine colour response index and the DE value of test saccharified liquid are the mass percent that reducing sugar accounts for syrup dry-matter, and question response carries out one section when not presenting iodine colour response again, until DE value is raised to 16, stops saccharification, and malt mash is moved in filter vat.In saccharifying, after crude amylase germinates Fructus Hordei Germinatus, remaining starch degradation is glucose and Oligomeric maltose (mainly comprising maltose, trisaccharide maltose, maltotetrose and above oligose); Proteolytic enzyme and lipase, make remaining protein and insoluble lipid material in Fructus Hordei Germinatus be degraded to soluble substance, finally forms malt mash.
The press filtration of step (3) malt mash is also made converted mash filtrate: it includes following steps:
A. pressure filtration: above-mentioned malt mash is moved in Fructus Hordei Germinatus grain filter pocket in the lump, start filter vat suction pump, carry out pressure filtration, 79 ℃ of washing trough water temps, Ph value 6.2, filters after 28min, start cultivated scouring machine and stir filter residue, add and wash poor water, again filter, the consumption of washing poor water is 8% of total inventory by mass percentage; Making to filter rear index reaches: filtrate dry-matter accounts for the more than 80% of total material dry-matter, filter residue water ratio 65%～75%, filtrate water ratio 75%～65%;
B. obtaining of converted mash filtrate: after press filtration, isolated filter residue is Fructus Hordei Germinatus grain, moves in Fructus Hordei Germinatus grain storage tank, after transport diary farm to and slightly raise; After press filtration, isolated filtrate is converted mash filtrate.Wherein, Fructus Hordei Germinatus grain dry-matter output weight accounts for and drops into 20% of Fructus Hordei Germinatus grain dry weight.In isolated converted mash filtrate, main dry-matter composition is dextrin, oligosaccharides, glucose, still has in addition a small amount of starch, protein, lipid material, solvable nitrogenous thing, amylan thing, mineral substance etc.Now, the quality percentage composition of converted mash filtrate dry-matter is 30%; Wherein solvable glucide accounts for 20%.
The coctoprecipitinogen of step (4) converted mash filtrate is also made maltose solution:
A. boiling and precipitation of converted mash filtrate: converted mash filtrate is proceeded in heating kettle, and be heated to 100 ℃, continue more than 20 minutes, until water transpiration 15% left and right, then be cooled to 15 ℃ of room temperatures, standing to being precipitated to flocculation sediment in bottom completely, it is clear that liquid becomes;
B. the extraction of maltose solution: after the solid substance that flocculates is deposited in described heating kettle bottom, release out supernatant liquor to retort, obtain maltose solution.The flocculation solid quality that this process is removed accounts for 10% of maltose solution total mass; The dry biomass percentage composition of maltose solution is increased to 36% because of concentrated.
The turning glycosides and make oligomeric isomaltose liquid of step (5) maltose solution:
A. the modulation of substrate solution: the maltose solution being generated by step (4), the Oligomeric maltose 36% that contains mass percent, regulates the ph to 5.2 of described maltose solution with acetic acid, and is heated to 58 ℃;
B. the selection of zymin and consumption: a-glucuroide and aspergillus oryzae a-amylase are dropped in described maltose solution, stir evenly, wherein the addition of a-glucuroide is 1TGU/g dry-matter; The diastatic addition of aspergillus oryzae a-is 3SKUB/g dry-matter.In the maltose solution that step (4) generates, the killing of the deactivation of enzyme, mushroom, the flocculation removal of protein macromolecule and the clarification of solution, for this enzymatic reaction provides condition.
C. reaction times and product monitoring: the glycosides that turns that continues above-mentioned steps a and b reacts 24h, after abstraction reaction liquid test passes, is warming up to 80 ℃ of inactivators, stopped reaction.The reaction solution extracting need be tested isomaltose (IG2), panose (P), Isomaltotriose (IG3), tetrose (Gn) content wherein.The total mass of oligomeric isomaltose in reaction solution (IG2+P+IG3+Gn) accounts for ratio >=50% of total reducing sugar butt quality, or be greater than total solution quality 17.5% time, stopped reaction.
Step (6) oligomeric isomaltose liquid concentrated and and make finished product.
A. evaporation concentration: temperature of reaction is raised to 100 ℃, evaporates the moisture of the mass percent 15% of total moisture content, make dry-matter be concentrated into mass percent 52%, the oligomeric isomaltose syrup that product becomes thick pulpous state is product;
B. the packing of product: be filled into sealing in 200L regular barrel, be finished product.
Claims (6)
1. a production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production, is characterized in that, it includes following steps: the production of (1) wheat malt is also made Fructus Hordei Germinatus serum; (2) saccharification of Fructus Hordei Germinatus serum make malt mash; (3) press filtration of malt mash make converted mash filtrate; (4) coctoprecipitinogen of converted mash filtrate make maltose solution; (5) maltose solution turns glycosides and makes oligomeric isomaltose liquid; (6) oligomeric isomaltose liquid concentrated and and make finished product; Wherein, the production of described step (1) wheat malt is also made Fructus Hordei Germinatus serum, include following steps: the choice criteria of a. wheat: selected wheat, except meeting the GB135186 regulation of brewageing wheat, also should meet following basic demand: moisture≤13%, percentage of germination >=90%, thousand seed weight >=35 gram, starch 57～64%, protein≤13%, germinating power >=85%, extraction rate >=82%, fat 1.5～23%;
B. wheat germination: get selected wheat by weight 1 times of above water soaking wheat 5～10 hours, after draining flowing water, put into germinating box, regulate the temperature inside the box to be retained to 15～20 ℃, spray water every day 2 times, germinate and carry out 2～4 days, temperature is risen to 18 ℃, continuing germinates again carries out 1～2 day, when now bud is grown average 3～5mm, stop germinateing, Fructus Hordei Germinatus outlet;
C. take off and must get grain: the wet Fructus Hordei Germinatus after outlet is put into propeller type mixer and stir 10～30 minutes, wheat root coring is fractureed and come off, sieve and remove cast, retain Fructus Hordei Germinatus grain standby;
D. colloidal mill grinds emulsification malt serum: above-mentioned Fructus Hordei Germinatus grain is put into colloidal mill, add the water of 1～1.2 times of amount of weight ratio, be milled to milk sap, make Fructus Hordei Germinatus serum.
2. a kind of production method of utilizing the feeding oligomeric isomaltose of Wheat Production slurry according to claim 1, it is characterized in that, the saccharification of described step (2) Fructus Hordei Germinatus serum is also made malt mash, it includes following steps: by the Fructus Hordei Germinatus serum making, according to following condition, process: saccharification temperature is at 62 ℃～68 ℃, pH value is adjusted to 6.0～6.5, saccharification time 1～2 hour, after be warming up to 78 ℃～80 ℃ of temperature; Iodine colour response index and the DE value of test saccharified liquid, question response carries out one section when not presenting iodine colour response again, until DE value is raised to 15～18, stops saccharification, and malt mash is moved in filter vat.
3. a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production according to claim 1, is characterized in that, the press filtration of described step (3) malt mash is also made converted mash filtrate: it includes following steps:
A. pressure filtration: above-mentioned malt mash is moved in Fructus Hordei Germinatus grain filter pocket in the lump, start filter vat suction pump, carry out pressure filtration, 78 ℃～80 ℃ of washing trough water temps, pH value 6.0～6.5, filters after 25～30min, start cultivated scouring machine and stir filter residue, add and wash poor water, again filter, the consumption of washing poor water is 5～10% of total inventory by mass percentage; Making to filter rear index reaches: filtrate dry-matter accounts for the more than 80% of total material dry-matter, filter residue water ratio 65%～75%, filtrate water ratio 75%～65%;
B. obtaining of converted mash filtrate: after press filtration, isolated filter residue is Fructus Hordei Germinatus grain, moves in Fructus Hordei Germinatus grain storage tank, after transport diary farm to and slightly raise; After press filtration, isolated filtrate is converted mash filtrate.
4. a kind of production method of utilizing the feeding oligomeric isomaltose slurry of Wheat Production according to claim 1, is characterized in that, the coctoprecipitinogen of described step (4) converted mash filtrate is also made maltose solution:
A. boiling and precipitation of converted mash filtrate: converted mash filtrate is proceeded in heating kettle, and be heated to 100 ℃, continue more than 15～30 minutes, to be then cooled to 15 ℃ of room temperatures, standing to being precipitated to flocculation sediment in bottom completely, it is clear that liquid becomes;
B. the extraction of maltose solution: after the solid substance that flocculates is deposited in described heating kettle bottom, release out supernatant liquor to retort, obtain maltose solution.
5. a kind of production method of utilizing the feeding oligomeric isomaltose of Wheat Production slurry according to claim 1, is characterized in that, the turning glycosides and make oligomeric isomaltose liquid of described step (5) maltose solution:
A. the modulation of substrate solution: the maltose solution being generated by step (4), the Oligomeric maltose 30～40% that contains mass percent, regulates pH to 5.0～5.5 of described maltose solution with acetic acid, and is heated to 55 ℃～60 ℃;
B. the selection of zymin and consumption: a-glucuroide and aspergillus oryzae a-amylase are dropped in described maltose solution and stirred evenly, and wherein the addition of a-glucuroide is 0.5～1.5TGU/g dry-matter; The diastatic addition of aspergillus oryzae a-is 1～5SKUB/g dry-matter;
C. reaction times and product monitoring: the glycosides that turns that continues above-mentioned steps a and b reacts 20～28h, after abstraction reaction liquid test passes, is warming up to 80 ℃ of inactivators, stopped reaction.
6. a kind of production method of utilizing the feeding oligomeric isomaltose of Wheat Production slurry according to claim 1, is characterized in that, described step (6) oligomeric isomaltose liquid concentrated and and make finished product.
A. evaporation concentration: temperature of reaction is raised to 100 ℃, evaporates the moisture of the mass percent 10～20% of total moisture content, make dry-matter be concentrated into mass percent 50～55%, the oligomeric isomaltose syrup that product becomes thick pulpous state is product;
B. the packing of product: be filled into sealing in 200L regular barrel, be finished product.
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| CN104855776A (en) * | 2015-05-15 | 2015-08-26 | 李燕 | Processing process for malt sugar |
| CN110214949A (en) * | 2018-03-03 | 2019-09-10 | 广州市合利源食品有限公司 | The extracting method of malt extract with flavouring and high nutritive value |
| CN113322295A (en) * | 2020-10-20 | 2021-08-31 | 河北肽丰生物科技有限公司 | Preparation method of selenium-rich triticale malt oligosaccharide peptide and product thereof |
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| MX2011008654A (en) * | 2010-08-24 | 2012-02-23 | Corn Products Int Inc | Production of isomaltooligosaccharides and uses therefor. |
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