WO2016206442A1 - Method for producing beer-brewing syrup based on corn direct method - Google Patents

Abstract

A method for producing beer-brewing syrup based on a corn direct method is specifically implemented according to the following steps: performing decrustation and degermination on corn, and preparing the corn into corn flour; performing syrup mixing treatment on the corn flour; liquefying the syrup-mixed corn flour; filtering liquefied liquid to remove residue; saccharifying the filtered filtrate to obtain saccharified liquid; performing decolored filtration on the saccharified liquid; performing ion exchange treatment on the decolored saccharified liquid; performing evaporation treatment on the saccharified liquid having undergone ion exchange; and performing finished product packaging, and preparing to obtain the finished beer-brewing syrup. Producing beer-brewing syrup by using corn instead of starch can increase a nitrogen source for beer fermentation, so as to improve beer flavor and quality, and can reduce the cost of sugar by 300-500 yuan per ton.

Description

Beer brewing syrup production method based on corn direct method Technical field

The invention belongs to the technical field of food fermentation, and particularly relates to a beer brewing syrup production method based on the direct corn method.

Background technique

Corn starch is the purest starch raw material for brewers. The reason why it is not widely used is that its price is more expensive than corn flour or brewed rice. However, the price of corn starch has been close to the price of rice in recent years. The brewer offers a good choice. The content of protein, fat and polyphenol in corn starch is very low. The use of corn starch as an auxiliary material can prolong the shelf life of beer, improve the flavor stability of beer and reduce the color of beer. Corn starch can be completely converted to soluble matter, so it does not cause filtration problems. The market prospects for corn starch applications depend to a large extent on their relative prices. The existing starch-based raw material for the production of beer-making syrup has the following problems: the corn starch process requires long-time immersion and water washing, and all the free protein precipitated is washed away, so that even if protease is added during saccharification, it is in the sugar liquid. There is no protein and it cannot be converted to nitrogen. Therefore, nitrogen sources cannot be provided for beer.

Summary of the invention

It is an object of the present invention to provide a beer brewing syrup production method based on the direct corn method, which solves the problem of poor flavor of beer brewing syrup beer existing in the prior art.

The technical scheme adopted by the invention is a beer brewing syrup production method based on the direct corn method, which is specifically implemented according to the following steps:

Step 1. peeling and degerming the corn to prepare corn flour;

Step 2. Performing a slurry treatment on the corn flour;

Step 3: liquefying the corn flour after the pulping;

Step 4: filtering and slaging the liquefied liquid;

Step 5, saccharifying the filtered filtrate to obtain a saccharification solution;

Step 6. Decolorize and filter the saccharification solution;

Step 7. Perform ion exchange treatment on the decolorized saccharified solution;

Step 8. Perform evaporation treatment on the ion exchanged saccharification solution;

Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.

The invention is also characterized in that

The corn flour prepared in the step 1 is specifically: fineness 40 mesh all passed, 60 mesh 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth stone, weed ≤ 1%; Corn flour fat content <0.1%; corn flour water <15%.

The step of adjusting the corn flour in the step 2 is specifically: adding a certain amount of food grade water in the mixing tank; adding steam to the temperature between 56 ° C and 60 ° C; adding CaCl ₂ according to the mass concentration of 0.1% - 0.5% _; After the mixing of the mixing tank is started, the corn powder is continuously added to the tank from the material silo, and the mass percentage of the corn flour and the food grade water is 38%-40%, and the pH of the corn flour is adjusted between 5.8-6.0, and then added. Ɑ-amylase, wherein the amount of ɑ-amylase added is 6 υ / g, and the mixture is uniform.

In step 3, the liquefaction of the corn flour after slurry adjustment is as follows: adjusting the steam supply pressure of the liquefier to 6 kg/cm ² , checking that the pipeline valve is in the working position, starting the slurry pump; opening the steam inlet of the jet liquefier Adjust the steam intake while opening the feed valve to control the feed flow rate; the injection temperature is always maintained at 106 °C-108 °C; the liquefied liquid after the injection enters the multiple liquefied liquid holding tanks in sequence, and stir for 20 minutes. After the sample, the DE value is measured, and the DE value is between 18% and 20%. The reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is 5.2 to 5.3.

In step 4, the liquefaction liquid is filtered and slag is specifically as follows: the oil pump is started to be compacted, filtered, and the feed pump is started, and the feed pressure is 3 kg/cm ² ; after the filtration is completed, the mass percentage of the residual sugar is washed by hot water. 3%, the obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.

In step 5, the filtered filtrate is saccharified to obtain a saccharification liquid. Specifically, the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm ² ; and the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger. Adjust the temperature to 52 ° C -55 ° C, adjust the pH between 5.2-5.3; add fungal enzyme, pullulanase and neutral protease in sequence and stir, among which, add fungal enzyme, pullulanase and neutral protease The amount is 2-4υ/g, 0.5L/M ³ saccharification solution and 0.1L/M ³ saccharification solution; stir for 2 minutes at intervals of 30 minutes; DE value is measured every 4 hours, and saccharification is terminated when DE ≥ 50 The saccharification solution has maltose ≥ 50%, maltotriose ≤ 23%, and glucose ≤ 10%.

In step 6, the saccharification solution is decolorized and filtered. Specifically, the activated carbon is added to the decolorization tank, and the mass ratio of the saccharification solution and the activated carbon is 100:2. After maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is <0.05. The loss rate is <2%.

The ion exchange treatment of the decolorized saccharified solution in step 7 is specifically as follows:

Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;

Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; Repeatedly soaking twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, after being washed with water to meet the requirements;

Step 7.3: Preparation of non-ionized water: using 5 times the volume of the tap water of the resin to flow through the anion and cation exchange columns from top to bottom, and measuring the conductivity of the water by using a conductivity meter for a plurality of times, and after being qualified, it is put into the ion-free water tank for use; When the conductivity of the effluent rises to 50 mn/cm ² , that is, when the resistance is lower than 20,000 ohms, the production of ion-free water is stopped, and regeneration is awaited; the cation and the cation resin are respectively washed with 5% alkali and acid, and then used in the ion-free water. Leaching the cation resin to PH=4, anion resin to PH=9, the resin column of the yin and yang resin is washed until the conductivity of the effluent reaches 50mn/cm ² or less, that is, when the resistance is more than 20,000 ohms;

Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm ² or less, and were used.

In step 8, the ion-exchanged saccharification solution is subjected to evaporation treatment, specifically: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm ² or more, and starting the feed pump, so that the saccharification liquid enters the first effect, the second effect, and the third. After the evaporation chamber is started, the vacuum pump is started to vacuum, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation. The sugar meter is often used to sample the concentration from the sampling port, and the concentration is required to be sent to the finished product tank. At the end of evaporation, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially.

The beneficial effects of the invention are: using corn instead of starch to produce beer brewing special syrup can make tons of sugar The cost is reduced by 300-500 yuan. The use of corn instead of starch to produce beer-making syrup can increase the nitrogen source for beer fermentation, and improve the beer flavor and improve the quality of beer.

detailed description

The present invention will be described in detail below in conjunction with specific embodiments.

The invention provides a beer brewing syrup production method based on the direct corn method, comprising the following steps:

Step 1. The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth and stone, weeds ≤ 1%; corn flour fat content < 0.1%; corn flour moisture < 15%.

Step 2: mixing the corn flour; adding a certain amount of food grade water in the slurry tank; adding steam to the temperature between 56 ° C and 60 ° C; adding CaCl ₂ according to the mass concentration of 0.1% - 0.5%; After the mixing tank is stirred, the corn powder is continuously added to the tank from the material silo, the mass percentage of corn flour and food grade water is 38%-40%, and after adjusting the pH between 5.8-6.0, the ɑ-amylase is added, The amount of addition was 6 υ / g, and the mixture was evenly stirred.

Step 3. Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm ² ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 106 ° C -108 ° C; the liquefied liquid after the injection into the liquefied liquid holding tank once in sequence, after stirring for 20 minutes, sampling The DE value is measured, and the DE value is between 18% and 20%. The reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is 5.2 to 5.3.

Step 4: Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm ² ; after the filtration, rinse with hot water and the percentage of residual sugar is <3% The obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.

Step 5: saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm ² ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted. The temperature is 52 ° C -55 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease respectively It is 2-4 υ / g, 0.5 L / M ³ saccharification solution and 0.1 L / M ³ saccharification solution; stirring for 2 minutes at intervals of 30 minutes; DE value is measured every 4 hours, saccharification is terminated when DE ≥ 50, and the obtained saccharification Maltose in the liquid ≥ 50%, maltotriose ≤ 23% and glucose ≤ 10%.

Step 6. Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is <0.05. The loss rate is <2%.

Step 7. Perform ion exchange treatment on the decolorized saccharified solution;

Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;

Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;

Step 7.3. Preparation of non-ionized water: use tap water of 5 times the volume of the resin to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm ² , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively. After washing, the cation resin is rinsed with ion-free water to PH=4, the anion resin to PH=9, and the resin column of the anode and the yang is washed until the conductivity of the effluent reaches 50 mn/cm ² or less, that is, when the resistance is 20,000 ohm or more;

Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm ² or less, and were used.

Step 8. Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm ² or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation. After the chamber, the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation; At the end, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;

Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.

The sensory index of the brewed syrup prepared by the above preparation process is:

Appearance: It is a viscous transparent liquid with no visible impurities;

Color: light yellow or slightly brownish yellow;

Aroma: has the normal smell of sugar for beer brewing;

Taste: Shurun is pure and has no odor.

The physical and chemical indicators of this beer brewing syrup are shown in Table 1:

Table 1 Physical and chemical indicators of beer brewing syrup

Indicator level	Gifted class	First level	Secondary
Solid content 1%	≥75	≥74	≥73
PH	5.2-5.3	5.2-5.3	5.2-5.3
DE%	≥50	≥52	≥55
Malt trisaccharide content 1%	≥23	≥21	≥20
Glucose content 1%	≥10	≥12	≥14
Ash 1%	≤0.5	≤0.8	≤1.2

The hygienic indicators of this beer brewing syrup are as follows:

Arsenic (mg/kg) ≤ 0.5

Lead (mg/kg) ≤ 1.0

The total number of bacteria (pieces / g) ≤ 3000

Coliform group (unit / 100g) ≤ 30

Pathogenic bacteria (intestinal pathogens and pathogenic cocci): none

Aflatoxin: None.

The technical achievement is based on the production of starch syrup, maltose syrup and fructose syrup on the basis of starch for many years. Through the practice of repeatedly using corn flour in production, the production of special syrup for beer brewing by direct corn production has been successfully developed. The process technology saves the link of sugar production after corn starch production, and achieves the purpose of reducing the cost of sugar production and improving economic benefits. At the same time, it provides high-quality brewing raw materials for the beer industry, saving a lot of grain for beer brewing, and reducing the production of beer production enterprises. In addition, the beer brewing syrup produced by the direct method of corn is rich in nitrogen source, which plays an important role in improving beer quality and improving beer flavor. This is not possible with starch as a raw material for the production of beer-making syrup. The reason is that the corn starch process requires long-term soaking and water washing, and all the free proteins precipitated are washed away, so that even if the protease is added during the saccharification, the protein cannot be converted into nitrogen because there is no protein in the sugar liquid. In the direct corn method, the corn flour is obtained by machine pulverization. When the corn is liquefied, there is a free protein precipitated. When the saccharification is added to the protease, nitrogen is produced. With the nitrogen source, the beer is better. The flavor, improve the quality of beer, bring better economic benefits to beer manufacturers.

Direct corn production of beer brewing syrup, so that the local conversion of food, the local appreciation is called the reality, which not only facilitates farmers to sell grain, but also opens up new ways for the value of agricultural products.

Five enzyme preparations are used in the process: amylase, fungal enzyme, pullulanase, protease, lipase; PH value has a great influence on the activity of the enzyme, and the pH value should be strictly controlled in the production, each enzyme The preparation has its own pH value under the best use condition, neither too low nor too high, otherwise it will affect the activity of the enzyme preparation and the efficiency of enzyme reaction. Therefore, in actual production, the PH value of the feed liquid should be strictly controlled. When the pH value must be adjusted, the pH should be adjusted first and then the enzyme should be added. The temperature of the enzyme preparation should be selected at the optimum temperature. The temperature should not be too low, but the enzyme reaction should be slow. If the temperature is too high, the enzyme denaturation will lose activity.

Example 1

A beer brewing syrup production method based on the direct corn method, comprising the following steps:

Step 1. The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth and stone, weeds ≤ 1%; corn flour fat content < 0.1%; corn flour moisture < 15%.

Step 2: Mixing the corn flour; adding a certain amount of food grade water in the pulping tank; adding steam to the temperature of 58 ° C; adding CaCl ₂ according to the mass concentration of 0.3%; and stirring the mixing tank The corn powder is added to the tank continuously from the material warehouse. The mass percentage of corn flour and food grade water is 39%. After the pH is adjusted between 5.8 and 6.0, the ɑ-amylase is added, and the addition amount is 6υ/g, and the mixture is evenly stirred. .

Step 3. Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm ² ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 107 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 23%, and the pH of the liquefied liquid is 5.2.

Step 4: Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm ² ; after the filtration, rinse with hot water and the percentage of residual sugar is <3% The obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.

Step 5: saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm ² ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted. The temperature is 54 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 3υ/ g, 0.5L/M ³ saccharification solution and 0.1L/M ³ saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ≥ 50, get maltose ≥ 50 in saccharification solution %, maltotriose ≤ 23% and glucose ≤ 10%.

Step 6. Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and residual sugar are <3%. At the end, the obtained filtrate was specifically colorless or yellowish in color, transmittance was >98%, color value was <0.05, and loss rate was <2%.

Step 7. Perform ion exchange treatment on the decolorized saccharified solution;

Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;

Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;

Step 7.3. Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm ² , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively. After washing, the cation resin is rinsed with ion-free water to PH=4, the anion resin to PH=9, and the resin column of the anode and the yang is washed until the conductivity of the effluent reaches 50 mn/cm ² or less, that is, when the resistance is 20,000 ohm or more;

Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm ² or less, and were used.

Step 8. Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm ² or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation. After the chamber, the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation; At the end, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;

Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.

Example 2

A beer brewing syrup production method based on the direct corn method, comprising the following steps:

Step 1. The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth and stone, weeds ≤ 1%; corn flour fat content < 0.1%; corn flour moisture < 15%.

Step 2: mixing the corn flour; adding a certain amount of food grade water in the mixing tank; adding steam to the temperature of 56 ° C; adding CaCl ₂ according to the mass concentration of 0.1%; after stirring the mixing tank, The material warehouse is continuously added with corn flour into the pool. The mass percentage of corn flour and food grade water is 38%. After the powder is added according to the output requirement, after adjusting the pH between 5.8 and 6.0, the ɑ-amylase is added. The amount of addition was 6 υ / g, and the mixture was evenly stirred.

Step 3. Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm ² ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 106 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is between 5.2 and 5.3.

Step 4: Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm ² ; after the filtration, rinse with hot water and the percentage of residual sugar is <3% The obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.

Step 5: saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm ² ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted. The temperature is 52 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 2υ/ g, 0.5L/M ³ saccharification solution and 0.1L/M ³ saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ≥ 50, get maltose ≥ 50 in saccharification solution %, maltotriose ≤ 23% and glucose ≤ 10%.

Step 6. Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is <0.05. The loss rate is <2%.

Step 7. Perform ion exchange treatment on the decolorized saccharified solution;

Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;

Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; Repeatedly soaking twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, after being washed with water to meet the requirements;

Step 7.3. Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm ² , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively. After washing, the cation resin is rinsed with ion-free water to PH=4, the anion resin to PH=9, and the resin column of the anode and the yang is washed until the conductivity of the effluent reaches 50 mn/cm ² or less, that is, when the resistance is 20,000 ohm or more;

Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm ² or less, and were used.

Step 8. Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm ² or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation. After the chamber, the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation; At the end, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;

Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.

Example 3

A beer brewing syrup production method based on the direct corn method, comprising the following steps:

Step 1. The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth and stone, weeds ≤ 1%; corn flour fat content < 0.1%; corn flour moisture < 15%.

Step 2: Mixing the corn flour; adding a certain amount of food grade water in the pulping tank; adding steam to the temperature of 60 ° C; adding CaCl ₂ according to the mass concentration of 0.5%; and stirring the mixing tank The corn powder is added to the tank continuously from the material warehouse. The mass percentage of corn flour and food grade water is 40%. After adjusting the pH between 5.8 and 6.0, the ɑ-amylase is added, and the addition amount is 6υ/g, and the mixture is evenly stirred. .

Step 3. Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm ² ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 108 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is between 5.2 and 5.3.

Step 4: Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm ² ; after the filtration, rinse with hot water and the percentage of residual sugar is <3% The obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.

Step 5: saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm ² ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted. The temperature is 54 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 4υ/ g, 0.5L/M ³ saccharification solution and 0.1L/M ³ saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ≥ 50, get maltose ≥ 50 in saccharification solution %, maltotriose ≤ 23% and glucose ≤ 10%.

Step 6. Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is <0.05. The loss rate is <2%.

Step 7. Perform ion exchange treatment on the decolorized saccharified solution;

Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;

Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;

Step 7.3. Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm ² , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively. After washing, the cation resin is rinsed with ion-free water to PH=4, the anion resin to PH=9, and the resin column of the anode and the yang is washed until the conductivity of the effluent reaches 50 mn/cm ² or less, that is, when the resistance is 20,000 ohm or more;

Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the yin from the top to the bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm ² or less, and were used.

Step 8. Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm ² or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation. After the chamber, the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation; At the end, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;

Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.

Claims (9)

1. A beer brewing syrup production method based on the direct corn method, which is characterized in that the following steps are specifically carried out:

   Step 1. peeling and degerming the corn to prepare corn flour;

   Step 2. Performing a slurry treatment on the corn flour;

   Step 3: liquefying the corn flour after the pulping;

   Step 4: filtering and slaging the liquefied liquid;

   Step 5, saccharifying the filtered filtrate to obtain a saccharification solution;

   Step 6. Decolorize and filter the saccharification solution;

   Step 7. Perform ion exchange treatment on the decolorized saccharified solution;

   Step 8. Perform evaporation treatment on the ion exchanged saccharification solution;

   Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.
2. The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the corn flour prepared in the step 1 is specifically: fineness of 40 meshes all passed, 60 meshes of 80% passed; germ, The skin yield is <18%; the corn flour yield is ≥80%; the earth and stone, weeds ≤1%; the corn flour fat content is <0.1%; the corn flour moisture is <15%.
3. The method for producing a beer brewing syrup based on the corn direct method according to claim 1, wherein the step of adjusting the corn flour in the step 2 is specifically: adding a certain amount of food grade water into the pulping tank; Add steam to the temperature between 56 °C and 60 °C; add CaCl ₂ according to the mass concentration of 0.1%-0.5%, start the mixing of the mixing tank, and then add corn flour into the pool continuously from the material silo, corn flour and food grade water. The mass percentage is 38%-40%. After adjusting the pH of the corn flour to 5.8-6.0, the ɑ-amylase is added, wherein the ɑ-amylase is added in an amount of 6 υ/g, and the mixture is evenly stirred.
4. The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the step of liquefying the pulverized corn flour is specifically: adjusting the liquefaction pressure of the liquefier to 6 kg/cm ² Check the pipeline valve in the working position, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust the steam intake, and open the feed valve to control the feed flow; the injection temperature is always maintained at 106 °C-108 °C; After the injection, the liquefied liquid enters a plurality of liquefied liquid holding tanks in sequence, and after stirring for 20 minutes, the DE value is sampled and measured, and the DE value is between 18% and 20%, indicating that the reaction is finished, and the liquefied liquid is obtained, and liquefaction is obtained. The concentration of the liquid is between 20% and 25%, and the pH of the liquefied liquid is between 5.2 and 5.3.
5. The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the step of filtering and slaging the liquefied liquid in the step 4 is: starting the oil pump to compact, filtering, starting the pump, and entering The pressure of the material is 3kg/cm ² ; after the filtration is completed, the filtration is carried out with hot water and the mass percentage of the residual sugar is <3%, and the obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.
6. The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein in the step 5, the filtered filtrate is saccharified to obtain a saccharifying liquid, which is specifically: steam autoclaving after washing the saccharification tank 20 minutes, wherein the pressure is 4kg/cm ² ; after the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, the temperature is adjusted to 52 ° C -55 ° C, and the pH is adjusted between 5.2 and 5.3; the fungal enzyme is added in turn. Lanase and neutral protease are stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 2-4υ/g, 0.5L/M ³ saccharification solution and 0.1L/M ³ saccharification solution; Stir for 2 minutes in 30 minutes; DE value is measured every 4 hours, and saccharification is terminated when DE ≥ 50, and the obtained saccharification solution has maltose ≥ 50%, maltotriose ≤ 23%, and glucose ≤ 10%.
7. The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the decolorizing and filtering of the saccharifying solution in the step 6 is specifically: adding a mass ratio of the activated carbon, the saccharifying solution and the activated carbon into the decolorizing tank. 100:2, after maintaining at 85 ° C for 30 minutes, drive into the filter Filtration starts within the filter, and after the light transmittance reaches 98% or more, the feed to the pre-discharge tank is started until the filtration and residual sugar <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, and the transmittance is transparent. >98%, color value <0.05, loss rate <2%.
8. The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the ion exchange treatment of the decolorized saccharified solution in the step 7 is as follows:

   Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;

   Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;

   Step 7.3: Preparation of non-ionized water: using 5 times the volume of the tap water of the resin to flow through the anion and cation exchange columns from top to bottom, and measuring the conductivity of the water by using a conductivity meter for a plurality of times, and after being qualified, it is put into the ion-free water tank for use; When the conductivity of the effluent rises to 50 mn/cm ² , that is, when the resistance is lower than 20,000 ohms, the production of ion-free water is stopped, and regeneration is awaited; the cation and the cation resin are respectively washed with 5% alkali and acid, and then used in the ion-free water. Leaching the cation resin to PH=4, anion resin to PH=9, the resin column of the yin and yang resin is washed until the conductivity of the effluent reaches 50mn/cm ² or less, that is, when the resistance is more than 20,000 ohms;

   Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm ² or less, and were used.
9. The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein in the step 8, the saccharification liquid after the ion exchange is subjected to evaporation treatment, specifically: adjusting the steam supply pressure of the evaporator to 5 -6kg/cm ² or more, start the pump, so that the saccharification liquid enters the one-effect, two-effect, three-effect evaporation chamber in turn, start the vacuum pump to vacuum, and the vacuum degree of each effect is sequentially achieved, and the steam valve is slowly opened. Evaporate, check the concentration from the sampling port with a sugar meter, and feed the finished product tank after the concentration reaches the requirement; at the end of the evaporation, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break The vacuum is turned off, the steam is turned off, and after 3 minutes of circulation, the machine is stopped in sequence.