CN102730841A - Method for degrading nitrobenzene in wastewater under condition of low temperature - Google Patents
Method for degrading nitrobenzene in wastewater under condition of low temperature Download PDFInfo
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- CN102730841A CN102730841A CN2012102359058A CN201210235905A CN102730841A CN 102730841 A CN102730841 A CN 102730841A CN 2012102359058 A CN2012102359058 A CN 2012102359058A CN 201210235905 A CN201210235905 A CN 201210235905A CN 102730841 A CN102730841 A CN 102730841A
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- nitrobenzene
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- mirbane
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Abstract
The invention relates to a method for degrading nitrobenzene in wastewater under condition of low temperature. The adopted technical scheme comprises the following steps of: adding active carbon of which the grain sizes are 1-3mm into wastewater under the temperature of 10-15DEG C and the initial nitrobenzene concentration of 200-400mg/L, wherein the adding quantity of active carbon is 0.3-0.6g/L; and inoculating nitrobenzene-degrading bacteria which are domesticated at low temperature during the exponential phase into the wastewater according to 1%-20% of volume percent, adjusting pH to 4-9, and cultivating for 48h in the case of vibrating. According to the method of the invention, nitrobenzene can be effectively degraded under low-temperature environment, the defect that low temperature restraints bacterial strain growth metabolism and nitrobenzene degrading capability can be overcome, the nitrobenzene degrading capability under low-temperature environment can be improved, and a technical support can be provided for the treatment of nitrobenzene under low-temperature environment.
Description
Technical field
The invention belongs to the environmental protection field, particularly a kind of fixedly method of oil of mirbane in the psychrotroph degrading waste water of biological activated carbon that adopts.
Background technology
Oil of mirbane is a kind of highly toxic substance of colourless or little yellow tool Semen Armeniacae Amarum flavor, is insoluble in water, is soluble in ethanol, ether, benzene and oil, and difficult for biological degradation, classified as world's " environment is preferentially controlled toxic organic pollutant ".It is a kind of important chemical material, is widely used in the production of medicine, agricultural chemicals, synthetic dyestuff, dyestuff, plastics and explosive.The fast development of whole world chemical industry constantly increases the demand of oil of mirbane, gets into the contaminants associated of environment thus and also increases thereupon, and a large amount of dischargings of nitrobenzene-containing waste water have been environmentally safe to have caused serious threat.When the concentration of oil of mirbane in the water body during at 5mg/L, it is yellow that contaminated water body is, and the Semen Armeniacae Amarum flavor is arranged.When concentration reached 100mg/L, water almost was black, and separated black precipitate, and when concentration surpasses 300mg/L, can cause fish and hydrobiont death.China requires the mass concentration≤2mg/L of nitrobenzene matters in the industrial water drainage, and drinking water standard then is 0.17mg/L, and therefore the research about nitrobenzene contamination water body reparation aspect enjoys people to pay close attention to.
At present; The treatment technology of oil of mirbane mainly contains physics method, chemical method and biological process; Biological process has advantages such as non-secondary pollution, expense be low, is the Perfected process of nitrobenzene degradation in the waste water, though isolated a large amount of nitrobenzene degradation bacterium in the prior art; But most of degradation bacteria is to degrade at normal temperatures, is not suitable for the degraded under the coldcondition.And in north China, the residing envrionment temperature of microbiological deterioration in winter oil of mirbane is very low, and the metabolism of most of mikrobe is in holddown, and the ability of degrading nitrobenzene also is restricted.
Summary of the invention
The objective of the invention is in order to solve the metabolic capacity difference problem of nitrobenzene degradation bacterium p-nitrophenyl under the low temperature environment; Make oil of mirbane in low temperature environment, can access preferably degraded, the present invention provides a kind of and can under coldcondition, have the method for efficient degradation oil of mirbane based on this purpose.
The technical scheme that the present invention adopts is: the method for oil of mirbane in the degrading waste water under a kind of coldcondition: under coldcondition, utilize biological activated carbon to fix and handle through the nitrobenzene degradation bacterium p-nitrophenyl waste water of domestication by low temperature.
The method of oil of mirbane in the degrading waste water under the above-mentioned coldcondition: in temperature is 10-15 ℃; The oil of mirbane starting point concentration is in the waste water of 200-400mg/L, and adding particle diameter is the gac of 1～3mm, and add-on is 0.3-0.6g/L; Volume percent by 1%～20%; The nitrobenzene degradation bacterium through domestication by low temperature of logarithmic phase is inoculated in the waste water, regulates pH=4～9, concussion was cultivated 48 hours down.
The method of oil of mirbane in the above-mentioned degrading waste water: described nitrobenzene degradation bacterium through domestication by low temperature is an Acinetobacter bauamnnii.
The present invention has following advantage:
1. economic environmental protection, existing physics, chemistry are repaired the technological method of nitrobenzene compounds polluted-water, can cause secondary pollution, and the harm ecosystem and cost are higher.And the present invention adopts bioremediation technology it is expensive little, can not cause secondary pollution, has good society, economy and ecological benefits.
2. the present invention adopts the method for the fixing nitrobenzene degradation bacterium through domestication by low temperature of biological activated carbon to come degrading nitrobenzene, rapidly and efficiently, simple to operate, energy-saving and cost-reducing, and has guaranteed a large amount of breedings of mikrobe, and degradation rate improves greatly.
3. the present invention is through the nitrobenzene degradation bacterium of domestication by low temperature, can be at growth metabolism under the lower temperature conditions, and degrading nitrobenzene preferably, guaranteed the degradation rate of oil of mirbane in low temperature environment.Adopt the present invention can be under 15 ℃ coldcondition effective degrading nitrobenzene, when the oil of mirbane starting point concentration was 200mg/L, degradation rate reached more than 60%.
Description of drawings
Fig. 1 is the atomic force microscope images of the Acinetobacter bauamnnii of acquisition.
Fig. 2 is the influence of inoculum size to degradation property.
Fig. 3 is the influence of pH value to degradation property.
Fig. 4 is the contrast of simple mikrobe and gac fixation of microbe p-nitrophenyl degradation effect.
Embodiment
Embodiment 1 is through the acquisition of the nitrobenzene degradation bacterium of domestication by low temperature
For making bacterial strain under coldcondition, have nitrobenzene degradation ability preferably, bacterial strain is carried out domestication by low temperature.Step is following:
(1) will receive the active sludge of nitrobenzene contamination behind enrichment culture 48h under 28 ℃ of temperature, the 150r/min condition, to get pregnant solution, with coating on the beef extract-peptone solid medium behind the supernatant gradient dilution.
(2) anti-oil of mirbane ability domestication: enrichment bacterium liquid is inoculated in minimal medium (potassium hydrogenphosphate 3.8g, potassium primary phosphate 1g, sodium-chlor 1g, ammonium chloride 0.1g, sal epsom 0.2g, the glucose 2g that contains the oil of mirbane saturated solution; Be made into 1000ml with zero(ppm) water) in carry out the anti-oil of mirbane ability domestication of bacterial strain; Progressively improve nitro phenenyl concentration in the domestication process; Progressively bring up to 200mg/L from 10mg/L; After cultivating 48h bacterium liquid is forwarded in the fresh liquid minimal medium (potassium hydrogenphosphate 3.8g, potassium primary phosphate 1g, sodium-chlor 1g, ammonium chloride 0.1g, sal epsom 0.2g, glucose 2g are made into 1000ml with zero(ppm) water).
(3) domestication by low temperature: the bacterium liquid after the oil of mirbane domestication that (2) step is obtained progressively carries out domestication by low temperature: under 25 ℃ condition, cultivate 48h, get bacterium liquid after the domestication and shift; Under 20 ℃ condition, cultivate 48h, shift the domestication back; Under 18 ℃ condition, cultivate 48h, shift the domestication back; Under 15 ℃ condition, cultivate 48h, shift the domestication back; Under 12 ℃ condition, cultivate 48h, shift the domestication back; Under 10 ℃ condition, cultivate 48h, shift the domestication back.
(4) then; With being coated with behind the bacterium liquid gradient dilution; Obtain the bacterial strain of anti-oil of mirbane under the coldcondition, carry out the bacterial strain purifying, obtain the low temperature resistant bacterium of oil of mirbane that p-nitrophenyl has degradation capability; Through thalli morphology, biochemical reactions characteristic and the analysis of 16SrDNA sequencing result is the Acinetobacter bauamnnii bacterial strain, and the result sees Fig. 1.The nitrobenzene degradation bacterium through domestication by low temperature that obtains is used for the following example.
The nitrobenzene degradation bacterium through domestication by low temperature of using in the inventive method; Its acquisition is not limited to the method for present embodiment; Also can adopt the Acinetobacter bauamnnii that has obtained in the prior art to belong to the nitrobenzene degradation bacterium; It is carried out the anti-oil of mirbane ability domestication of present embodiment the 2nd step and the progressively domestication by low temperature of the 3rd step, and thus obtained nitrobenzene degradation bacterium all can be used for method of the present invention.
Embodiment 2 inoculum sizes are to the influence of degradation property
Method: with OD
600=2 the nitrobenzene degradation bacterium bacteria suspension through domestication by low temperature is seeded to the minimal medium that nitro phenenyl concentration is 200mg/L (potassium hydrogenphosphate 3.8g, potassium primary phosphate 1g, sodium-chlor 1g, ammonium chloride 0.1g, sal epsom 0.2g, glucose 2g by 1%, 5%, 10%, 15% and 20% volume percent; Be made into 1000ml with zero(ppm) water) in; (particle diameter 1 ~ 3mm), concussion was cultivated 48 hours under 15 ℃, pH=7,150r/min condition to add the 0.5g granular active carbon.Utilize the nitrobenzene degradation rate of gas Chromatographic Determination bacterial strain 48h, degradation results such as Fig. 2.
As shown in Figure 2, along with the increase of inoculum size, the degradation rate of oil of mirbane increases thereupon, but when inoculum size during in 10% ~ 20% range, the increase of inoculum size is little to the growing state and the degradation capability influence of bacterial strain, and therefore, the preferred inoculum size of the present invention is 10%.
Embodiment 3 pH values are to the influence of degradation property
Method: will be in logarithmic phase to be seeded to the pH value through the nitrobenzene degradation bacterium bacteria suspension of domestication by low temperature respectively by 10% (v/v) be that 4,5,6,7,8,9 nitro phenenyl concentration is minimal medium (potassium hydrogenphosphate 3.8g, potassium primary phosphate 1g, sodium-chlor 1g, ammonium chloride 0.1g, sal epsom 0.2g, the glucose 2g of 200mg/L; Be made into 1000ml with zero(ppm) water) in; (particle diameter 1 ~ 3mm), 48h is cultivated in concussion under 15 ℃, 150r/min condition to add the 0.5g granular active carbon.Utilize the nitrobenzene degradation rate of gas Chromatographic Determination bacterial strain 48h, degradation results such as Fig. 3.
As shown in Figure 3, when the pH value increased to 7 by 4, the degradation rate of oil of mirbane significantly raise, when pH greater than 7 the time, bacterial strain has been played restraining effect, degradation rate sharply descends, so the preferred pH value of the present invention is 7.
Embodiment 4
The nitrobenzene degradation bacterium bacteria suspension through domestication by low temperature that will be in logarithmic phase is seeded to respectively by 10% (v/v) and adds 0.5g granular active carbon (particle diameter 1 ~ 3mm) and the minimal medium (potassium hydrogenphosphate 3.8g, potassium primary phosphate 1g, sodium-chlor 1g, ammonium chloride 0.1g, sal epsom 0.2g, the glucose 2g that do not add gac; Be made into 1000ml with zero(ppm) water) in; The starting point concentration of oil of mirbane is 200mg/L; Regulating pH value is 7, concussion cultivation 48h under 15 ℃, 150r/min condition.Utilize the nitrobenzene degradation rate of gas Chromatographic Determination bacterial strain 48h, contrast the degradation rate of simple mikrobe and gac fixation of microbe, degradation results such as Fig. 4.
As shown in Figure 4, utilize and degrade through the nitrobenzene degradation bacterium of domestication by low temperature, degradation rate reaches 63.8%, and adopts gac to fix the degraded through the nitrobenzene degradation bacterium p-nitrophenyl of domestication by low temperature, and its degradation rate can reach 89.9%, compares and has improved 26.1%.
The nitrobenzene degradation bacterium bacteria suspension through domestication by low temperature that will be in logarithmic phase is inoculated in the minimal medium that the oil of mirbane starting point concentration is 400mg/L (potassium hydrogenphosphate 3.8g, potassium primary phosphate 1g, sodium-chlor 1g, ammonium chloride 0.1g, sal epsom 0.2g, glucose 2g by 10% (v/v); Be made into 1000ml with zero(ppm) water) in; (particle diameter 1 ~ 3mm), concussion was cultivated 48 hours under 15 ℃, pH=7,150r/min condition to add the 0.5g granular active carbon.Utilize the nitrobenzene degradation rate of gas Chromatographic Determination bacterial strain 48h, recording degradation rate is 82.8%.
The method of oil of mirbane in the degrading waste water under embodiment 6 coldcondition
The nitrobenzene degradation bacterium bacteria suspension through domestication by low temperature that will be in logarithmic phase is inoculated in the 1000ml waste water that the oil of mirbane starting point concentration is 200mg/L by 10% (v/v); (particle diameter 1 ~ 3mm), concussion was cultivated 48 hours under 10 ℃, pH=7,150r/min condition to add the 0.5g granular active carbon.Utilize the nitrobenzene degradation rate of gas Chromatographic Determination bacterial strain 48h, recording degradation rate is 89.9%.
Claims (3)
1. the method for oil of mirbane in the degrading waste water under the coldcondition is characterized in that: utilize under coldcondition that biological activated carbon is fixing to be handled through the nitrobenzene degradation bacterium p-nitrophenyl waste water of domestication by low temperature.
2. according to the method for oil of mirbane in the degrading waste water under the described coldcondition of claim 1, it is characterized in that: in temperature is 10-15 ℃, and the oil of mirbane starting point concentration is in the waste water of 200-400mg/L; Adding particle diameter is the gac of 1～3mm; Add-on is 0.3-0.6g/L, by 1%～20% volume percent, the nitrobenzene degradation bacterium through domestication by low temperature of logarithmic phase is inoculated in the waste water; Regulate pH=4～9, concussion was cultivated 48 hours down.
3. according to the method for oil of mirbane in claim 1 or the 2 described degrading waste waters, it is characterized in that: described nitrobenzene degradation bacterium through domestication by low temperature is an Acinetobacter bauamnnii.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105217777A (en) * | 2015-11-05 | 2016-01-06 | 江苏天伦活性炭有限公司 | A kind of water treatment biological activated carbon preparation technology flow process |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1765772A (en) * | 2005-08-31 | 2006-05-03 | 东华工程科技股份有限公司 | Disposal method of nitrobenzene removal from water |
| RU2424197C2 (en) * | 2009-10-07 | 2011-07-20 | Государственное образовательное учреждение высшего профессионального образования Иркутский государственный университет | Method for detoxification of aquatic medium contaminated with nitrobenzene |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1765772A (en) * | 2005-08-31 | 2006-05-03 | 东华工程科技股份有限公司 | Disposal method of nitrobenzene removal from water |
| RU2424197C2 (en) * | 2009-10-07 | 2011-07-20 | Государственное образовательное учреждение высшего профессионального образования Иркутский государственный университет | Method for detoxification of aquatic medium contaminated with nitrobenzene |
Non-Patent Citations (2)
| Title |
|---|
| 孙凌 等: "活性炭固定耐冷菌对硝基苯的降解特性", 《中国环境科学》, vol. 29, no. 9, 30 September 2009 (2009-09-30) * |
| 李银萍: "硝基苯耐低温降解菌株的筛选及降解性能研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》, 15 December 2010 (2010-12-15) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105217777A (en) * | 2015-11-05 | 2016-01-06 | 江苏天伦活性炭有限公司 | A kind of water treatment biological activated carbon preparation technology flow process |
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Application publication date: 20121017 |