CN1952105A - Process for preparing microorganism species for treating dyeing waste water - Google Patents
Process for preparing microorganism species for treating dyeing waste water Download PDFInfo
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- CN1952105A CN1952105A CN 200610154577 CN200610154577A CN1952105A CN 1952105 A CN1952105 A CN 1952105A CN 200610154577 CN200610154577 CN 200610154577 CN 200610154577 A CN200610154577 A CN 200610154577A CN 1952105 A CN1952105 A CN 1952105A
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Abstract
The invention disclosed a preparing process of microbial population which can be used to treat dying waste liquids. Collect the engineering primitive microbial population from the sewage or silt on the discharging point of the dying factory. Enrich the microbe by inoculating the primitive microbial population on the medium for 5-7d to get the engineering microbial population. Add 5%, 10%, 15%, 20% and 25% dying waste liquids to the microbe medium and habituated cultivate the microbe according to the microbe's cultivating method for 7-10d to get habituated microbial population. The invention has many advantages such as economical, efficient, safe, great adaptivity and no secondary pollution, so it's an ideal microbial population to treat dying waste liquids.
Description
Technical field
The present invention relates to a kind of preparation method of microorganism species of treatment of dyeing and printing, be fit to be applied to the processing of the waste water that printing and dyeing mill discharges.
Background technology
Along with the development of dyestuff and printing and dyeing industry, the waste water of its production has become one of current topmost pollution source of water body.The composition of dyeing waste water is very complicated, and dye material is selected different, and the waste water composition is also just different.In general, the main component of dyeing waste water is the mixture of compounds such as azo dyes, anthraquinone dyes and aniline, oil of mirbane, phenols, the document that sees reference (Chen Yifei, Shi Chengliang, dyeing waste water composition analysis and purification treatment technology, Sichuan silk, 2002 (3), 15-17.)。Dyeing waste water not only produces visual impact to environment, and majority be the organism of poisonous difficult degradation, and its chemical stability is strong, has carcinogenic, teratogenesis, mutagenic effect, will produce the potential threat to the mankind and biology.Treatment of dyeing wastewater adopts physico-chemical processes and conventional biological treatment more at present.There is the working cost height in physico-chemical processes, can produces secondary pollution problems.In the conventional biological treatment because the complex construction and the toxicity of degraded object, cause conventional microorganism metabolism to be subjected to bigger inhibition, aerobic bacteria is very limited to the Degradation of hardly degraded organic substance, though anaerobic bacterium is effective to some organic matter degradation, but the stronger organic danger of stupid amine of the toxicity of generation is arranged, and aniline is difficult to further degraded.So it is unsatisfactory that the ordinary method cultured microorganism is used for the treatment effect of dyeing waste water.The preparation method who researches and develops a kind of efficient, economy, adaptability is strong, dyeing waste water is degraded on ecological safety ground microorganism species is necessary.
Summary of the invention
The purpose of this invention is to provide a kind of can be economical, efficient, adaptability is strong, ecological safety ground treatment of dyeing and printing, the useful decomposition mushroom in the local environment is had guide and the preparation method of the microorganism species of synergistic treatment of dyeing and printing.
It is to collect the original flora of engineering from the sewage of the Sewage outlet of printing and dyeing mill and mud, it is inoculated into according to method for culturing microbes carries out enrichment culture in the microbiological culture media, cultivate and obtained the engineering bacteria flora in 5-7 days, then the engineering bacteria flora is tamed cultivation, the dyeing waste water that needs are handled is added in the microbiological culture media by 5%, 10%, 15%, 20%, 25% different load, tame cultivation according to method for culturing microbes, cultivate and obtained the acclimated microorganism flora in 7-10 days.
The beneficial effect that the present invention has is:
(1) relies on mutual synergy between these microorganisms of being cultivated, can form a Stability Analysis of Structures, wide adaptability, the microorganism species that degradation capability is strong;
(2) cultured microbial bacteria group energy directly comes into operation, and need not to experience long laundering period again, can promptly play the effect of degraded dyeing waste water;
Economy of the present invention, efficient, ecological security is high, suitability is wide, non-secondary pollution, is the desirable microorganism species of treatment of dyeing and printing.
Embodiment
The preparation method of the microorganism species of treatment of dyeing and printing is:
(1) collection of the original flora of engineering
Find through secular working practice and scientific experiment, near source of pollution, exist a large amount of beneficial microbe strain resources, they can adapt to contaminant species and bear the major responsibility of degrade this contaminant species and environment purification, therefore be one of the main means of obtaining the flora of this contaminant species of degrading efficiently from gathering flora near the source of pollution, (Shen Dezhong writes the document that sees reference, the biological restoration of contaminate environment, Chemical Industry Press, Beijing, 2001,163-174).The present invention collects the original flora of engineering from the sewage of the Sewage outlet of 4 printing and dyeing mills of printing and dyeing industry garden, Shaoxing and mud.Hydrostatic layer collection water sample in the aseptic wide-necked bottle is adopted in the collection of water sample; The collection of mud is to collect 50-100 gram sample from mud, places sterile vessel, and all samples all should carry out enrichment culture in 24 hours, or preserved at 4 ℃ of lower seals.
(2) enrichment culture of the original flora of engineering
The original flora of engineering that collects is inoculated into according to method for culturing microbes carries out enrichment culture in the microbiological culture media.Monitor the pH value in the nutrient solution every day,, repeat 3 times, cultivate and to obtain the engineering bacteria flora in 5-7 days when the pH value is 4.0 good with regard to enrichment culture when following.
(3) domestication of engineering bacteria flora is cultivated
Tame cultivation with increasing the method for mixing dyeing waste water (mixture of 4 dyeing waste waters that printing and dyeing mill discharged of printing and dyeing industry garden, Shaoxing, its principal pollutant matter is anthraquinone dyes, aniline, oil of mirbane, phenol etc.) concentration in the nutrient solution gradually.The dyeing waste water that needs are handled is added in the microbiological culture media by 5%, 10%, 15%, 20%, 25% different load, tame cultivation according to method for culturing microbes, observe the nutrient solution change in color every day, take off fully up to color, repeat 3 times, cultivate and to obtain the acclimated microorganism flora in 7-10 days.Find that after tested the microorganism species after the domestication comprises 3.52 * 10
9Cell/ml, wherein milk-acid bacteria 40%, yeast 30%, photosynthetic bacteria 15%, actinomycetes 10%, mould 5%.
Described microbiological culture media is a 2.38g/L starch, 4.74g/L glucose, 2.25g/L sucrose, 1.65g/L fructose, 11.14g/L ammonium nitrate, 1.00g/L saltpetre, 0.01g/L seven water sulfurous acid iron, 0.50g/L magnesium sulfate heptahydrate, 1.00g/L potassium primary phosphate, 0.01g/L Calcium Chloride Powder Anhydrous, all the other are sterile distilled water.
Described microbial culture method is the microbiological culture media that adds 100mL in the 250mL Erlenmeyer flask, inserts 10% microorganism species, and Erlenmeyer flask is placed in the aseptic processing room, and at 25 ℃, pH6.5-7.5 opens wide static cultivation down.
Claims (3)
1. the preparation method of the microorganism species of a treatment of dyeing and printing, it is characterized in that: from the sewage of the Sewage outlet of printing and dyeing mill and mud, collect the original flora of engineering, it is inoculated into according to method for culturing microbes carries out enrichment culture in the microbiological culture media, cultivate and obtained the engineering bacteria flora in 5-7 days, then the engineering bacteria flora is tamed cultivation, the dyeing waste water that needs are handled is pressed 5%, 10%, 15%, 20%, 25% different load is added in the microbiological culture media, tame cultivation according to method for culturing microbes, cultivate and obtained the acclimated microorganism flora in 7-10 days.
2. the preparation method of the microorganism species of a kind of treatment of dyeing and printing according to claim 1, it is characterized in that: described microbiological culture media is a 2.38g/L starch, 4.74g/L glucose, 2.25g/L sucrose, 1.65g/L fructose, 11.14g/L ammonium nitrate, 1.00g/L saltpetre, 0.01g/L seven water sulfurous acid iron, 0.50g/L magnesium sulfate heptahydrate, 1.00g/L potassium primary phosphate, the 0.01g/L Calcium Chloride Powder Anhydrous, all the other are sterile distilled water.
3. the preparation method of the microorganism species of a kind of treatment of dyeing and printing according to claim 1, it is characterized in that: described method for culturing microbes is for adding the microbiological culture media of 100mL in the 250mL Erlenmeyer flask, insert 10% engineering flora, Erlenmeyer flask is placed in the aseptic processing room, at 25 ℃, pH6.5-7.5 opens wide static cultivation down.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200610154577 CN1952105A (en) | 2006-11-08 | 2006-11-08 | Process for preparing microorganism species for treating dyeing waste water |
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| CN 200610154577 CN1952105A (en) | 2006-11-08 | 2006-11-08 | Process for preparing microorganism species for treating dyeing waste water |
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| CN1952105A true CN1952105A (en) | 2007-04-25 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914440A (en) * | 2010-07-06 | 2010-12-15 | 浙江省环境保护科学设计研究院 | Method for massively culturing decolorizing bacteria of dyeing and printing wastewater |
| CN105417708A (en) * | 2016-01-11 | 2016-03-23 | 新疆德蓝股份有限公司 | Method for processing and adjusting printing and dyeing wastewater through biochemical method |
| CN105540997A (en) * | 2015-12-12 | 2016-05-04 | 常州大学 | Method for treating nitrobenzene wastewater |
| CN107597067A (en) * | 2017-10-10 | 2018-01-19 | 孙祎 | A kind of preparation method for the treatment of of dyeing/printing wastewaters |
| CN110616168A (en) * | 2019-08-29 | 2019-12-27 | 扬州联智生物科技有限公司 | Construction method and application of multi-extremophile microbial community for degrading dye |
| CN110628665A (en) * | 2019-08-20 | 2019-12-31 | 扬州联智生物科技有限公司 | Halophilic and alkalophilic microbial flora and application thereof |
| CN110628667A (en) * | 2019-08-29 | 2019-12-31 | 扬州联智生物科技有限公司 | Construction method and application of halophilic thermophilic microbial community for degrading dye |
-
2006
- 2006-11-08 CN CN 200610154577 patent/CN1952105A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914440A (en) * | 2010-07-06 | 2010-12-15 | 浙江省环境保护科学设计研究院 | Method for massively culturing decolorizing bacteria of dyeing and printing wastewater |
| CN105540997A (en) * | 2015-12-12 | 2016-05-04 | 常州大学 | Method for treating nitrobenzene wastewater |
| CN105417708A (en) * | 2016-01-11 | 2016-03-23 | 新疆德蓝股份有限公司 | Method for processing and adjusting printing and dyeing wastewater through biochemical method |
| CN107597067A (en) * | 2017-10-10 | 2018-01-19 | 孙祎 | A kind of preparation method for the treatment of of dyeing/printing wastewaters |
| CN110628665A (en) * | 2019-08-20 | 2019-12-31 | 扬州联智生物科技有限公司 | Halophilic and alkalophilic microbial flora and application thereof |
| CN110616168A (en) * | 2019-08-29 | 2019-12-27 | 扬州联智生物科技有限公司 | Construction method and application of multi-extremophile microbial community for degrading dye |
| CN110628667A (en) * | 2019-08-29 | 2019-12-31 | 扬州联智生物科技有限公司 | Construction method and application of halophilic thermophilic microbial community for degrading dye |
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