CN103864226B - A kind of cultivation of the Granular sludge for the treatment of percolate and acclimation method - Google Patents
A kind of cultivation of the Granular sludge for the treatment of percolate and acclimation method Download PDFInfo
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- CN103864226B CN103864226B CN201410090545.6A CN201410090545A CN103864226B CN 103864226 B CN103864226 B CN 103864226B CN 201410090545 A CN201410090545 A CN 201410090545A CN 103864226 B CN103864226 B CN 103864226B
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Abstract
The present invention relates to a kind of cultivation and acclimation method of the Granular sludge for the treatment of percolate.First by the vaccination target of soil under rotted leaf as process percolate, additional appropriate glucose, completes the cultivation of Granular sludge; Stop adding glucose, carry out Granular sludge domestication, water outlet COD, at below 200mg/L, realizes the nitrogen removal rate of about 70%.Progressively promote the load of pending percolate after sludge acclimatization success, the COD of the water outlet of pending percolate all can reach below 200mg/L, achieves effective process of the aged percolate of centering.It is high that the present invention has vaccination target specific aim, and mud starts fast, the advantages such as anti-foreign impacts ability is strong, sedimentation effect is good, and can be widely used in the unmanageable wastewater treatment of other traditional biological methods.
Description
Technical field
The present invention relates to a kind of cultivation and acclimation method of the Granular sludge for the treatment of percolate, specifically a kind of reluctant containing high-concentration organic substance wastewater for the treatment of traditional biological method, as the high specific aim microbial inoculant of landfill yard mature landfill leachate and Granular sludge are cultivated and acclimation method.Belong to technical field of biological treatment of wastewater.
Background technology
Treatment process mainly leading portion anaerobic technique+aerobic process+bis-membrane process of current percolate.Processing cost is high, and operation is complicated.This technique can produce the concentrated solution of high-enriched organics, is more difficult to process.Simultaneously because C/N in percolate is lower, the demand of traditional Biochemical method to nutrition-allocated proportion can not be met.For this reason, a lot of scholar develops new technique: such as synchronous nitration and denitrification, short-cut nitrification and denitrification, the techniques such as Anammox, irrefragablely be, these techniques in laboratory even part on a small scale all achieve extraordinary effect in engineering, saved working cost, but these techniques have a common shortcoming: comparatively strict to requirement for environmental conditions.Also have the new technologies such as high-level oxidation technology equally also to there is the problem of condition harshness, the optimum PH range of such as Fenton's reaction is about 3.0, and this almost can not realize in Practical Project.And ozonation technology is owing to preparing the high price of ozone, engineering staff is also made to hang back.The garbage leachate treatment process of other new lives has: microbial battery treating refuse percolate, but this method is still faced with very large difficulty on the percolate of process ammonia nitrogen in high density at present.For high-concentration organic substance wastewater---percolate process field, anaerobic technique is generally as pretreatment technology.The anaerobic technique of current application mainly comprises: UASB, EGSB, IC and ASBR etc.Anaerobic technique is for being vital by wastewater treatment to meeting subsequent technique to water quality requirement.
Anaerobism water treatment to refer at dissolved oxygen (DO) at below 0.2mg/L at activated sludge process.Anaerobic technique is stricter for the requirement of operational conditions, and temperature requirement is higher, practical application difficulty.And anaerobic technique is long for start time.Anaerobic technique traditional equally can not obtain obvious effect for the process of the organic waste water of difficult for biological degradation.Anaerobic grain activated sludge process becomes the focus of a research in field of waste water treatment.Because granular mud has tolerance, organic loading is high, and anti-load impact ability is strong, and microbial population enriches, the advantages such as sedimentation function is good, is a very promising technique in process high concentrated organic wastewater field.But unfortunately the cultivation domestication process of granule sludge is more difficult, cycle time long (more than 3 months).If can realize the quick startup of anaerobic technique, and obtain good organic removal rate, meanwhile include the advantages such as the shock resistance of granule sludge, sedimentation function are good, that will be a wonderful news for the process containing high-concentration organic substance wastewater.
Chinese patent CN101125718A discloses a kind of method processing middle and advanced stage percolate: carry out adsorbing thus the organism removed in percolate with the wilkinite improved after first adopting struvite crystalline deposit method removing ammonia nitrogen.This technology is physical chemistry method, and still having very large wilkinite etc. after enforcement needs process further; Chinese patent CN101125724A discloses the method for aged percolate in a kind of Biochemical method, upflowing UBF-BAF immobilized microorganism combination process is adopted to achieve higher COD clearance, but water outlet COD is still at about 400mg/L, still higher to follow-up processing requirement.As middle aged percolate water outlet COD controlled at below 200mg/L by a kind of biological process, will be good news to percolate treatment industry.The inoculation overwhelming majority of the active sludge of existing process percolate is from city domestic sewage treatment plant, and bacterial classification is wherein very limited for the degradation capability of the organism kinds of percolate.Strong, adaptable microorganism realizes percolate screening organic matter degradation ability, and especially, aged percolate carries out effectively process is necessary.Utilize corresponding soil directly to inoculate, the aged percolate of the Granular sludge centering that fast culture goes out can realize high specific aim, effectively process, and these class methods are not appeared in the newspapers.
Chinese patent CN103265111A discloses in one the method accelerating anaerobic granulation.Simultaneously the method adds granulated active carbon and polyquaternium in mud mixed system; by improving particulate matter and microorganism Contact and attachment to accelerate the enrichment of microorganism at surface particles; make it the core carrier forming granule sludge; and then form granular mud form, the stability of system and sedimentation function are all increased.Chinese patent CN102557248A discloses a kind of method of anaerobic grain sludge fast culture.The method makes the anaerobic grain sludge of original fragmentation or waddingization again form the anaerobic grain sludge of efficient stable fast by adding gac and polyacrylamide etc.But these patents are all carry out granular sludge at additional mud or on original basis, and have application limitation, carry out inoculation domestication as still needed a large amount of similar mud of inoculation for specific wastewater treatment.Utilize corresponding soil directly to inoculate, the Granular sludge that fast culture goes out can realize high specific aim to the waste water of specifying, process efficiently, and these class methods are not appeared in the newspapers.
Summary of the invention
The object of the invention is to be directed to the existing difficult problem faced for percolate process: activated sludge process organism tolerance load is not high, physico-chemical process cost is high and operational conditions is strict etc., the present invention attempts adopting a kind of domestication carrying out mud to the soil ulmin high soil microorganisms inoculation culture mud of degraded specific aim and in conjunction with the characteristic of granule sludge, and then realize the macromolecular effective degraded of soil ulmin, improve the biodegradability of waste water, to can aerobic process or other advanced treatment process auxiliary under complete the process up to standard of the aged percolate of centering.
In order to achieve the above object, the present invention adopts following technique:
A, covers more than 3 years from trees leaf-fall and does not suffer IA to get earth, and this earth is the soil of microorganism growth attachment, adds in earth by distilled water or tap water, stirs, and preparation obtains the soil turbid solution that form is viscous state;
B, dropped in sequencing batch reactor by soil turbid solution, the dosage of soil turbid solution is 0.4 ~ 0.75/10 of reactor useful volume; Then by middle aged percolate input sequencing batch reactor; And glucose is also dropped into wherein; With dilute hydrochloric acid solution, pH is controlled between 7 ~ 8 again, water temperature is at 15 ~ 25 DEG C, press 150r/min continuously to stir, the 3/10(hydraulic detention time that every day is reactor useful volume into and out of the amount of percolate controlled at 3 ~ 4 days), cultivate 18-22 days, mud metamorphosis in observing response device, continuing when there is floc sludge to run 5 days according to above-mentioned condition, completing the cultivation of Granular sludge;
Above-mentioned glucose adds once every day, and the amount at every turn adding glucose deducts half chemical oxygen demand (COD) content by 20 times of total nitrogen content in middle aged percolate, is then multiplied by the flooding quantity of middle aged percolate:
Above-mentioned middle aged percolate is the consumer waste filling and embedding time limit percolate of more than 5 years, and COD is 500 ~ 1000mg/L, TN is 200 ~ 300mg/L;
C, stop adding glucose, enter Granular sludge domestication: temperature of reactor is adjusted to 22 ~ 25 DEG C, and with dilute hydrochloric acid solution, pH value is controlled to be 7 ~ 8, continue to stir 18-20 days by above-mentioned speed, every day enters, the amount going out percolate is 3/10 of reactor useful volume, utilize illumination and reactor water outlet, inlet flow-patterm checks Granular sludge domestication success or not and confirms: first check Granular sludge domestication, i.e. Continuous Observation mode of appearance of trickle soil particle in mud in reactor under strong illumination condition, in the mud at initial stage, soil particle there will be reflective phenomenon under strong illumination, when soil particle being wrapped up completely after microorganism increment to a certain extent, no-reflection phenomenon after strong illumination, mud form is between traditional floc sludge and granule sludge, do not observe soil particle, then the water outlet of detection reaction device and water inlet pH value, reach fair, confirm that this Granular sludge is tamed successfully, now, the clearance of percolate water outlet COD is 75%, and water outlet COD is down to below 200mg/L,
D, Granular sludge to tame successfully the pending percolate load progressively promoted as intaking, the COD concentration of first stage water inlet is between 1000 ~ 1500mg/L, after mensuration water outlet COD drops to below 200mg/L, again inlet COD concentration is increased to 1500 ~ 2500mg/L, by that analogy, need the COD concentration value (water outlet COD drops to below 200mg/L) all up to standard of pending percolate to be processed, in this process, to control the stage of all taming with Granular sludge identical for the condition such as hydraulic detention time, temperature, stirring.
In above-mentioned steps A, earth is chosen requirement and is: region of fetching earth is experience the place that more than 3 years leaf-falls cover at least continuously, and the fallen leaves piled up obviously rot, and soil is not contaminated.Push rotted leaf aside, more moistening soil under getting rotted leaf, the degree of depth that fetches earth is no more than 5cm.The pedotheque obtained is loaded in an open container, the distilled water about adding relative to soil sample 5 times of volumes, stir, there is not larger soil particle.Then the suspended substance such as elimination turbid solution upper strata leaf and grass roots.Again stir, pour out fast after stopping about about 2/3 turbid solution (in viscous state) for subsequent use to another one open container, remaining heavier residue is outwelled.Repeat above step, until obtain enough soil turbid solutions (being at least 1/20 of sbr reactor device useful volume).
When above-mentioned B walks glucose dosage calculating in Granular sludge cultivation, first day flooding quantity is calculated with aged percolate gauge in input actual in reactor.
Advantage of the present invention and effect are;
1, be rich in various bacterial classification humic acid material to height endurability because the present invention adopts in the soil being in rotted leaf covering for a long time, and these bacterial classification survival abilities are superpower; In addition in aged percolate, the organism of more than 90% is humic acid material, so adopt such soil to carry out the pointed height of introduction of bacterial classification, the active advantage such as strong is also that the present invention fast culture can go out the basic reason of target mud.
2, because the soil fine particle in soil turbid solution of the present invention is that microorganism enrichment provides good attachment point, therefore can by irradiating with torch, a large amount of thread fungus anaerobions because occurring in observing response device water sample are attached on tiny soil particle, and do not occur that reflective spot judges that Granular sludge is cultivated domestication and terminated.
3, the present invention does not introduce any mud and originates as bacterial classification in culturing sludge process, only in culturing sludge process, add a small amount of glucose, the glucose amount added is according to water inlet percolate COD and TN numerical value, do not consider the demand of TP, microorganism is 40:1 for the demand ratio of C, N, is that 50% pair of daily requirement is estimated with the clearance of COD and TN.
4, the present invention tames utilization inoculation soil incubation mud out and no longer adds glucose, and the percolate adopted in this process is still aged percolate, but COD load needs to raise, and starts conventional parameter as DO, pH in sludge acclimatization process, ORP, Inlet and outlet water COD, BOD
5, NH
3-N, TN, NO
x -the indexs such as-N carry out record, and maintain an equal level with inlet flow-patterm, and this phenomenon illustrates that sludge acclimatization process terminates.Also can by carrying out the judgement of domestication process to the observation of soil particle form in reactor.
5, in the present invention, the existence of soil molecule makes Granular sludge sedimentation function very good, there is not water outlet sludge loss situation, the culturing process accelerating mud it also avoid the work of sludge treatment, it is good that the anaerobic grain shape mud obtained has settling property, anti-load impact ability is strong, to advantages such as hardly degraded organic substance tolerance are strong; And start time is short, unloading phase gentle for the requirement of envrionment conditions, simple, do not need to add a large amount of nutritive salt, pH and temperature etc. require all lower, are very suitable for engineer applied.
Accompanying drawing explanation
Fig. 1 is time and water-in and water-out pH value change curve in Granular sludge domestication process of the present invention
Fig. 2 is time and Inlet and outlet water COD content and clearance change curve in Granular sludge domestication process of the present invention
Embodiment
A, from a pine tree rotted leaf, shallow soil layer takes soil sample, is loaded in 250mL beaker by the pedotheque of about 20g, adds the distilled water of about 150mL, stirs, makes there is not larger soil particle in beaker.Then the suspended substance such as elimination turbid solution upper strata leaf and grass roots.Again stir, stop the turbid solution (in viscous state) pouring out about about 2/3 after stirring fast for subsequent use to another one 250mL beaker, remaining heavier residue is outwelled.Repeat above step, until obtain about about 90mL soil turbid solution.With the large glass beaker of 2L as ASBR reactor, and soil turbid solution is poured in beaker, about adding percolate to 1800mL graticule.
B, culturing sludge process water inlet percolate COD at about 200mg/L at about 500mg/L, TN, pass in and out the water yield every day and are 500mL(hydraulic detention time and control at 3 ~ 4 days), through calculating, daily requirement adds 2.0g glucose.Mechanical stirring speed control at about 150r/min to ensure the Homogeneous phase mixing of organism and mud in reactor.Within 21st day, observe floc sludge at reactor to occur, continue cultivation 5 days.And in the process of culturing sludge, Mei Gesan, four days water samplings carry out bacterial classification observation under the microscope.Occur after mud, occur a large amount of thread fungus in water sample, this provides the condition of necessity for growth on anaerobion is attached to tiny soil particle.After this in order to carry out the domestication of active sludge, no longer glucose is added.
C, the COD value of the aged percolate adopted in Granular sludge domestication process at about 800mg/L, TN at about 300mg/L.Be placed in water-bath by reactor in domestication process, in maintenance reactor, water temperature is at about 22 DEG C.With rare HCL solution, pH value of solution in reactor is maintained between 7 ~ 8, after Granular sludge tames 18 days (from 09-December 26 December), after reactor water outlet pH and inlet flow-patterm maintain an equal level, no longer artificially regulate pH.This phenomenon confirms that sludge acclimatization process terminates, and sees Fig. 1, and after December 26, water outlet pH maintains an equal level with water inlet, and water outlet COD also obviously declines, and sees figure .2.In observing response device, soil particle form, irradiates reactor with torch, and in time there is not reflective spot in mud, illustrate that all soil particles are all wrapped up by microorganism, now sludge acclimatization terminates.Experimental result to show in domestication process the clearance of the highest COD of realization being 75%, water outlet COD maintains below 200mg/L, sees figure .2, but unfortunately data are only recorded to 12.29, afterwards because percolate is forced to stop for quantity not sufficient experiment, but suffice to show that the validity of experiment; TN is the highest have about 70% clearance, ammonia nitrogen finally realizes the clearance of more than 88%.
Although do not carry out aeration operation at experimentation, but along with the propelling of Granular sludge domestication, in percolate, dissolved oxygen fluctuation is risen, to make in percolate (as table .1) between dissolved oxygen maintains 0.2 ~ 0.5, percolate is in anoxic condition, and this will be conducive to denitrifying carrying out.From 12.16 days, dissolved oxygen maintained more than 0.2mg/L always afterwards, ironically water outlet NH after 12.16 days
3the concentration of-N and TN also starts significantly to cut down, and has confirmed denitrifying existence.
Dissolved oxygen (DO) and ammonia nitrogen (NH in table .1 reactor operational process
3-N) change
D, progressively promotes the pending percolate load as water inlet, and need the COD concentration value of percolate to be processed all up to standard, namely water outlet COD maintains below 200mg/L.
Claims (3)
1., for the treatment of cultivation and the acclimation method of the Granular sludge of percolate, it is characterized in that:
A, covers more than 3 years from trees leaf-fall and does not suffer IA to get earth, and this earth is the soil of microorganism growth attachment, adds in earth by distilled water or tap water, stirs, and preparation obtains the soil turbid solution that form is viscous state;
B, dropped in sequencing batch reactor by soil turbid solution, the dosage of soil turbid solution is 0.4 ~ 0.75/10 of reactor useful volume; Then by middle aged percolate input sequencing batch reactor; And glucose is also dropped into wherein; With dilute hydrochloric acid solution, pH is controlled between 7 ~ 8 again, at 15 ~ 25 DEG C, press 150r/min continuously to stir, every day, the amount into and out of percolate was 3/10 of reactor useful volume, cultivate 18-22 days, mud metamorphosis in observing response device, continuing when there is floc sludge to run 5 days according to above-mentioned condition, completing the cultivation of Granular sludge;
Above-mentioned glucose adds once every day, and the amount at every turn adding glucose deducts half chemical oxygen demand (COD) content by 20 times of total nitrogen content in middle aged percolate, is then multiplied by the flooding quantity of middle aged percolate:
Above-mentioned middle aged percolate is the consumer waste filling and embedding time limit percolate of more than 5 years, and COD is 500 ~ 1000mg/L, TN is 200 ~ 300mg/L;
C, stop adding glucose, start Granular sludge domestication: temperature of reactor is adjusted to 22 ~ 25 DEG C, and with dilute hydrochloric acid solution, pH value is controlled to be 7 ~ 8, continue to stir 18-20 days by above-mentioned speed, every day enters, the amount going out percolate is 3/10 of reactor useful volume, utilize illumination and reactor water outlet, inlet flow-patterm checks Granular sludge domestication success or not and confirms: first by Continuous Observation mode of appearance of trickle soil particle in mud in reactor under strong illumination, check Granular sludge domestication progress, namely, in the mud at initial stage, soil particle there will be reflective phenomenon under strong illumination, when soil particle being wrapped up completely after microorganism increment to a certain extent, no-reflection phenomenon after strong illumination, mud form is between traditional floc sludge and granule sludge, do not observe soil particle, then the water outlet of detection reaction device and water inlet pH value, reach fair, confirm that this Granular sludge is tamed successfully, now, the clearance of percolate water outlet COD is 75%, and water outlet COD is down to below 200mg/L,
D, Granular sludge to tame successfully the pending percolate load progressively promoted as intaking, the COD concentration of first stage water inlet is between 1000 ~ 1500mg/L, after mensuration water outlet COD drops to below 200mg/L, then inlet COD concentration is increased to 1500 ~ 2500mg/L, by that analogy, the water outlet COD of pending percolate all maintains below 200mg/L, hydraulic detention time in this process, temperature, all tame the stage with Granular sludge identical with the control of stirring.
2. the cultivation of a kind of Granular sludge for the treatment of percolate according to claim 1 and acclimation method, it is characterized in that: in steps A, earth choosing method is, push rotted leaf aside, more moistening soil under getting rotted leaf, the degree of depth that fetches earth is no more than 5cm, the earth sample obtained is loaded in an open container, add the distilled water relative to earth sample 5 times of volumes, stir, there is not larger soil particle, then elimination turbid solution upper strata leaf and grass roots, again stir, pour out 2/3 for subsequent use to another one open container in the turbid solution of viscous state after stopping fast, remaining heavier residue is outwelled.
3. the cultivation of a kind of Granular sludge for the treatment of percolate according to claim 1 and acclimation method, it is characterized in that: the Granular sludge of described B step cultivate in glucose dosage when calculating, the flooding quantity of first day is calculated with the gauge of aged percolate in input actual in reactor.
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| CN104843864A (en) * | 2015-06-02 | 2015-08-19 | 广西大学 | Method for improving stability of landfill leachate anaerobic granular sludge |
| CN105217783B (en) * | 2015-11-17 | 2017-05-31 | 陕西惠瑞环保科技工程有限公司 | A kind of method of the culture of anaerobic activated sludge |
| CN108002530A (en) * | 2017-12-22 | 2018-05-08 | 中国海洋大学 | A kind of cultural method for the anaerobic grain sludge for handling the waste water containing polyacrylamide |
| CN113149229B (en) * | 2021-02-24 | 2023-03-21 | 青岛理工大学 | Pure membrane MBBR starting method with soil as inoculation system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101306870A (en) * | 2008-07-07 | 2008-11-19 | 中国科学院成都生物研究所 | Heterotrophic nitrification aerobic granule sludge, culture method thereof and use |
| FR2945039A1 (en) * | 2009-04-30 | 2010-11-05 | Ondeo Ind Solutions | Anaerobic digestion of water from liquid phase containing suspension materials e.g. co-product, comprises subjecting wort to solid/liquid separation phases, and extracting main product from first vegetable material transformed into wort |
| CN102040281A (en) * | 2009-10-21 | 2011-05-04 | 中国石油化工股份有限公司 | Method for culturing denitrogenized granular sludge |
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| JPS62279894A (en) * | 1986-05-30 | 1987-12-04 | Kobe Steel Ltd | Formation of granular sludge in anaerobic treatment process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101306870A (en) * | 2008-07-07 | 2008-11-19 | 中国科学院成都生物研究所 | Heterotrophic nitrification aerobic granule sludge, culture method thereof and use |
| FR2945039A1 (en) * | 2009-04-30 | 2010-11-05 | Ondeo Ind Solutions | Anaerobic digestion of water from liquid phase containing suspension materials e.g. co-product, comprises subjecting wort to solid/liquid separation phases, and extracting main product from first vegetable material transformed into wort |
| CN102040281A (en) * | 2009-10-21 | 2011-05-04 | 中国石油化工股份有限公司 | Method for culturing denitrogenized granular sludge |
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