CN103864226A - Culturing and domesticating method for treating granular sludge of leachate - Google Patents
Culturing and domesticating method for treating granular sludge of leachate Download PDFInfo
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Abstract
The invention relates to a culturing and domesticating method for treating granular sludge of leachate. The method comprises the following steps: firstly, with the soil under rotted leaves serving as an inoculation target of the treated leachate and by adding a proper amount of glucose, culturing the granular sludge; stopping glucose addition and domesticating the granular sludge, wherein the COD (Chemical Oxygen Demand) of effluent is lower than 200mg/L and the total nitrogen removal rate achieves about 70%. After the sludge is successfully domesticated, the load of a to-be-treated leachate is gradually enhanced, the COD of effluents of all the to-be-treated leachates can be lower than 200mg/L, and aged-old leachates are effectively treated. The method has the advantages that the targeting performance of the inoculation target is high, the sludge start-up speed is high, the external impact resisting capability is strong, the sedimentation effect is good, and the like; the method can be widely used for treatment of wastewater which is difficult to treat by other traditional biological approaches.
Description
Technical field
The present invention relates to a kind of cultivation and acclimation method of the particulate state mud for the treatment of percolate, specifically a kind of reluctant containing high-concentration organic substance wastewater, as high specific aim microbial inoculant and particulate state culturing sludge and the acclimation method of landfill yard mature landfill leachate for the treatment of traditional biological method.Belong to biological wastewater treatment technology field.
Background technology
The treatment process of percolate is mainly leading portion anaerobic technique+aerobic process+bis-membrane process at present.Processing cost is high, operation complexity.This technique can produce the concentrated solution of high-enriched organics, is more difficult to process.Because C/N in percolate is lower, can not meet traditional biological process and process the demand to nutrition-allocated proportion simultaneously.For this reason, a lot of scholars have developed new technique: such as synchronous nitration and denitrification, short-cut nitrification and denitrification, the techniques such as Anammox, irrefragablely be, these techniques have even all obtained extraordinary effect in part small-scale engineering in laboratory, saved working cost, but these techniques have a common shortcoming: comparatively strict to requirement for environmental conditions.Also have the new technology such as high-level oxidation technology equally also to exist the problem of condition harshness, such as the best pH scope of Fenton's reaction is in 3.0 left and right, this almost can not realize in Practical Project.And ozonation technology is owing to preparing the high price of ozone, also make engineering staff hang back.Other newborn garbage leachate treatment process have: microbial battery treating refuse percolate, but this method is still faced with very large difficulty on the percolate of processing ammonia nitrogen in high density at present.For high-concentration organic substance wastewater---percolate process field, anaerobic technique is generally as pretreatment technology.The anaerobic technique of application mainly comprises at present: UASB, EGSB, IC and ASBR etc.Anaerobic technique is for being vital to meeting subsequent technique to water quality requirement by wastewater treatment.
Anaerobism water treatment refer to dissolved oxygen (DO) below 0.2mg/L at activated sludge process.Anaerobic technique is stricter for the requirement of operational conditions, and temperature requirement is higher, practical application difficulty.And anaerobic technique is long start time.Same traditional anaerobic technique can not obtain obvious effect for the processing of the organic waste water of difficult for biological degradation.Anaerobic grain activated sludge process becomes the focus of a research in field of waste water treatment.The advantages such as organic loading is high because granular mud has tolerance, and anti-load impact ability is strong, and microbial population is abundant, and sedimentation function is good are a very promising technique processing high concentrated organic wastewater field.But the cultivation domestication process of not enough is granule sludge is more difficult, cycle time long (more than 3 months).If can realize the quick startup of anaerobic technique, and obtain good organic removal rate, meanwhile include the advantages such as shock resistance, the sedimentation function of granule sludge is good, that will be a wonderful news for the processing containing high-concentration organic substance wastewater.
Chinese patent CN101125718A discloses a kind of method of processing middle and advanced stage percolate: adsorb with improved wilkinite the organism of removing in percolate thereby first adopt struvite crystalline deposit method to remove after ammonia nitrogen.This technology is physical chemistry method, still has the further processing such as very large wilkinite after enforcement; Chinese patent CN101125724A discloses the method for aged percolate in a kind of biological process processing, adopt upflowing UBF-BAF immobilized microorganism combination process to obtain higher COD clearance, but water outlet COD is still in 400mg/L left and right, still higher to follow-up processing requirement.If middle aged percolate water outlet COD is controlled at below 200mg/L by a kind of biological process, will be good news to percolate treatment industry.The inoculation overwhelming majority of the active sludge of existing processing percolate is from city domestic sewage treatment plant, and bacterial classification is wherein very limited for the degradation capability of the organism kinds of percolate.Screening organic matter degradation ability strong, adaptable microorganism is realized percolate, especially in aged percolate to carry out effectively processing be necessary.Utilize corresponding soil directly to inoculate, the aged percolate of particulate state mud centering that fast culture goes out can be realized high specific aim, effectively process, and these class methods are not appeared in the newspapers.
Chinese patent CN103265111A discloses and in one, has accelerated the granular method of anaerobic sludge.The method is to add granulated active carbon and polyquaternium in mud mixed system simultaneously; by improving particulate matter and microorganism Contact and adhering to accelerate the enrichment of microorganism on particulate matter surface; make it to form the core carrier of granule sludge; and then form granular mud form, the stability of system and sedimentation function are all increased.Chinese patent CN102557248A discloses a kind of method of anaerobic grain sludge fast culture.The method is to make the anaerobic grain sludge of original fragmentation or waddingization again form fast the anaerobic grain sludge of efficient stable by adding gac and polyacrylamide etc.But these patents are all to carry out granular sludge at additional mud or on original basis, and have application limitation, inoculate a large amount of similar mud inoculate domestication as still needed for specific wastewater treatment.Utilize corresponding soil directly to inoculate, the particulate state mud that fast culture goes out can be realized high specific aim, process efficiently the waste water of specifying, and these class methods are not appeared in the newspapers.
Summary of the invention
The object of the invention is to be directed to the existing difficult problem that processing faces for percolate: activated sludge process organism tolerance load is not high, physico-chemical process cost is high and operational conditions is strict etc., the present invention attempts adopting a kind of to the high soil microorganisms inoculation culture mud of soil ulmin degraded specific aim and carry out the domestication of mud in conjunction with the characteristic of granule sludge, and then realize the macromolecular effective degraded of soil ulmin, improve the biodegradability of waste water, to can complete the processing up to standard of the aged percolate of centering under aerobic process or other advanced treatment process auxiliary.
In order to achieve the above object, the present invention adopts following technique:
A, covers more than 3 years and does not suffer IA to get earth from trees leaf-fall, and this earth is the soil of microorganism growth attachment, and distilled water or tap water are added in earth, stirs, and preparation obtains the soil turbid solution that form is viscous state;
B, drops into soil turbid solution in sequencing batch reactor, and the dosage of soil turbid solution is 0.4～0.75/10 of reactor useful volume; Then by middle aged percolate input sequencing batch reactor; And glucose is also dropped into wherein; With dilute hydrochloric acid solution, pH is controlled between 7～8 again, water temperature is at 15～25 ℃, pressing continuously 150r/min stirs, the 3/10(hydraulic detention time that be reactor useful volume into and out of the amount of percolate every day is controlled at 3～4 days), cultivate 18-22 days, mud metamorphosis in observing response device continues, according to above-mentioned condition operation 5 days, to complete the cultivation of particulate state mud in the time there is floc sludge;
Above-mentioned glucose adds once every day, and the amount that at every turn adds glucose is to deduct half chemical oxygen demand (COD) content by 20 times of total nitrogen content in middle aged percolate, is then multiplied by the flooding quantity of middle aged percolate:
Above-mentioned middle aged percolate is 5 years above percolates of the consumer waste filling and embedding time limit, and COD is 500～1000mg/L, and TN is 200～300mg/L;
C, stop adding glucose, enter particulate state sludge acclimatization: temperature of reactor is adjusted into 22～25 ℃, and with dilute hydrochloric acid solution by pH value control be 7～8, continue to stir 18-20 days by above-mentioned speed, enter every day, the amount that goes out percolate is 3/10 of reactor useful volume, utilize illumination and reactor water outlet, water inlet pH checks and confirms particulate state sludge acclimatization success or not: first check particulate state sludge acclimatization, it is Continuous Observation mode of appearance of trickle soil particle in mud in reactor under strong illumination condition, in the mud at initial stage, soil particle there will be reflective phenomenon under strong illumination, when soil particle being wrapped up completely after microorganism increment to a certain extent, no-reflection phenomenon after strong illumination, mud form is between traditional floc sludge and granule sludge, do not observe soil particle, then the water outlet of detection reaction device and water inlet pH value, reach fair, confirm this particulate state sludge acclimatization success, now, the clearance of percolate water outlet COD is 75%, and water outlet COD is down to below 200mg/L,
D, after particulate state sludge acclimatization success, progressively promote the pending percolate load as water inlet, the COD concentration of first stage water inlet is between 1000～1500mg/L, after mensuration water outlet COD drops to below 200mg/L, again inlet COD concentration is increased to 1500～2500mg/L, by that analogy, need all (water outlet COD drop to 200mg/L following) up to standard of COD concentration value of pending percolate to be processed, in this process, the condition control such as hydraulic detention time, temperature, stirring is all identical with the particulate state sludge acclimatization stage.
In above-mentioned steps A earth choose require be: the region of fetching earth be experience at least continuously more than 3 years leaf-fall cover place, and pile up fallen leaves obviously rot, soil is not contaminated.Push rotted leaf aside, get more moistening soil under rotted leaf, the degree of depth that fetches earth is no more than 5cm.The pedotheque of obtaining is packed in an open container, add the distilled water with respect to 5 times of volume left and right of soil sample, stir, do not have larger soil particle.Then the suspended substance such as elimination turbid solution upper strata leaf and grass roots.Again stir, stop rear turbid solution (being viscous state) that pouring out fast about 2/3 left and right to for subsequent use in another one open container, remaining heavier residue is outwelled.Repeat above step, until obtain enough soil turbid solutions (be at least sbr reactor device useful volume 1/20).
When in above-mentioned B step particulate state culturing sludge, glucose dosage calculates, first day flooding quantity is to calculate with the middle aged diafiltration liquid measure of actual input in reactor.
Advantage of the present invention and effect are;
1, be rich in various bacterial classifications humic acid material to height endurability because the present invention adopts in the soil covering in rotted leaf for a long time, and these bacterial classification survival abilities are superpower; In addition in aged percolate, more than 90% organism is humic acid material, so adopt such soil to carry out the pointed height of introduction of bacterial classification, the active advantage such as strong, is also the basic reason that the present invention can fast culture goes out target mud.
2, because the soil fine particle in soil turbid solution of the present invention provides good attachment point for microorganism enrichment, therefore can be by irradiating with torch, in observing response device water sample, because a large amount of thread fungus anaerobions that occur are attached on tiny soil particle, and do not occur that reflective spot judge the domestication end of particulate state culturing sludge.
3, the present invention does not introduce any mud and originates as bacterial classification in culturing sludge process, only in culturing sludge process, add a small amount of glucose, the glucose amount adding is according to water inlet percolate COD and TN numerical value, do not consider the demand of TP, microorganism is for C, and the demand ratio of N is 40:1, estimates take the clearance of COD and TN as 50% pair of daily requirement.
4, the present invention no longer adds glucose to utilizing inoculation soil incubation mud out to tame, and the percolate adopting in this process is still aged percolate, but COD load needs to raise, in sludge acclimatization process, start to conventional parameter as DO pH, ORP, Inlet and outlet water COD, BOD
5, NH
3-N, TN, NO
x -the indexs such as-N are carried out record, and maintain an equal level with water inlet pH, and this phenomenon explanation sludge acclimatization process finishes.Also can be by the observation of soil particle form in reactor being tamed to the judgement of process.
5, in the present invention, the existence of soil molecule makes particulate state sludge settling performance very good, there is not water outlet sludge loss situation, the culturing process of having accelerated mud has also been avoided the work of sludge treatment, it is good that the anaerobic grain shape mud obtaining has settling property, anti-load impact ability is strong, to advantages such as hardly degraded organic substance tolerance are strong; And start time is short, unloading phase for the requirement gentleness of envrionment conditions, simple, do not need to add a large amount of nutritive salt, pH and temperature etc. require all lower, are very suitable for engineering application.
Accompanying drawing explanation
Fig. 1 is time and water-in and water-out pH value change curve in particulate state sludge acclimatization process of the present invention
Fig. 2 is time and Inlet and outlet water COD content and clearance change curve in particulate state sludge acclimatization process of the present invention
Embodiment
A, from a pine tree rotted leaf, shallow soil layer is taked soil sample, and the pedotheque of about 20g is packed in 250mL beaker, adds the distilled water of 150mL left and right, stirs, and makes not exist in beaker larger soil particle.Then the suspended substance such as elimination turbid solution upper strata leaf and grass roots.Again stir, the turbid solution (being viscous state) that stops pouring out fast about 2/3 left and right after stirring, to for subsequent use in another one 250mL beaker, is outwelled remaining heavier residue.Repeat above step, until obtain about 90mL left and right soil turbid solution., and soil turbid solution is poured in beaker as ASBR reactor with the large glass beaker of 2L, added percolate to 1800mL graticule left and right.
B, culturing sludge process water inlet percolate COD is in 500mg/L left and right, TN is in 200mg/L left and right, passes in and out the water yield every day and is 500mL(hydraulic detention time and is controlled at 3～4 days), through calculating, daily requirement adds 2.0g glucose.Mechanical stirring speed control in about 150r/min to guarantee the even mixing of organism and mud in reactor.Within the 21st day, observe floc sludge at reactor and occur, continue to cultivate 5 days.And in the process of culturing sludge, carry out under the microscope bacterial classification observation every three, four days water samplings.Occur after mud, occurring a large amount of thread funguss in water sample, this provides necessary condition for growth on anaerobion is attached to tiny soil particle.After this in order to carry out the domestication of active sludge, no longer add glucose.
C, the COD value of the aged percolate adopting in particulate state sludge acclimatization process is in 800mg/L left and right, and TN is in 300mg/L left and right.In domestication process, reactor is placed in water-bath, maintains the interior water temperature of reactor 22 ℃ of left and right.PH value of solution in reactor is maintained between 7～8 with rare HCL solution, 18 days (from 09-December 26 December) of particulate state sludge acclimatization, reactor water outlet pH no longer artificially regulates pH after maintaining an equal level with water inlet pH.This phenomenon confirms that sludge acclimatization process finishes, and sees figure .1, and after December 26, water outlet pH maintains an equal level with water inlet, and water outlet COD also obviously declines, and sees figure .2.Soil particle form in observing response device, irradiates reactor with torch, illustrates that all soil particles are all wrapped up by microorganism in the time there is not reflective spot in mud, and now sludge acclimatization finishes.Experimental result shows in domestication process that the clearance of the highest COD of realization is 75%, water outlet COD maintains below 200mg/L, sees figure .2, but deficiency is that data are only recorded to 12.29, afterwards because percolate is forced to stop for quantity not sufficient experiment, but suffice to show that the validity of experiment; The highest clearance that has 70% left and right of TN, ammonia nitrogen is finally realized more than 88% clearance.
Although do not carry out aeration operation at experimentation, but along with the propelling of particulate state sludge acclimatization, in percolate, dissolved oxygen fluctuation is risen, make in percolate (as table .1) between dissolved oxygen maintains 0.2～0.5, percolate is in anoxic condition, and this will be conducive to denitrifying carrying out.More than from 12.16 days, dissolved oxygen maintained 0.2mg/L always afterwards, ironically water outlet NH after 12.16 days
3the concentration of-N and TN also starts significantly to subdue, and has confirmed denitrifying existence.
Dissolved oxygen (DO) and ammonia nitrogen (NH in table .1 reactor operational process
3-N) variation
D, progressively promotes the pending percolate load as water inlet, needs the COD concentration value of percolate to be processed all up to standard, and water outlet COD maintains below 200mg/L.
Claims (3)
1. for the treatment of cultivation and the acclimation method of the particulate state mud of percolate, it is characterized in that:
A, covers more than 3 years and does not suffer IA to get earth from trees leaf-fall, and this earth is the soil of microorganism growth attachment, and distilled water or tap water are added in earth, stirs, and preparation obtains the soil turbid solution that form is viscous state;
B, drops into soil turbid solution in sequencing batch reactor, and the dosage of soil turbid solution is 0.4～0.75/10 of reactor useful volume; Then by middle aged percolate input sequencing batch reactor; And glucose is also dropped into wherein; With dilute hydrochloric acid solution, pH is controlled between 7～8 again, at 15～25 ℃, pressing continuously 150r/min stirs, be 3/10 of reactor useful volume into and out of the amount of percolate every day, cultivate 18-22 days, mud metamorphosis in observing response device continues, according to above-mentioned condition operation 5 days, to complete the cultivation of particulate state mud in the time there is floc sludge;
Above-mentioned glucose adds once every day, and the amount that at every turn adds glucose is to deduct half chemical oxygen demand (COD) content by 20 times of total nitrogen content in middle aged percolate, is then multiplied by the flooding quantity of middle aged percolate:
Above-mentioned middle aged percolate is 5 years above percolates of the consumer waste filling and embedding time limit, and COD is 500～1000mg/L, and TN is 200～300mg/L;
C, stop adding glucose, start particulate state sludge acclimatization: temperature of reactor is adjusted into 22～25 ℃, and with dilute hydrochloric acid solution by pH value control be 7～8, continue to stir 18-20 days by above-mentioned speed, enter every day, the amount that goes out percolate is 3/10 of reactor useful volume, utilize illumination and reactor water outlet, water inlet pH checks and confirms particulate state sludge acclimatization success or not: first by Continuous Observation mode of appearance of trickle soil particle in mud in reactor under strong illumination, check particulate state sludge acclimatization progress, , in the mud at initial stage, soil particle there will be reflective phenomenon under strong illumination, when soil particle being wrapped up completely after microorganism increment to a certain extent, no-reflection phenomenon after strong illumination, mud form is between traditional floc sludge and granule sludge, do not observe soil particle, then the water outlet of detection reaction device and water inlet pH value, reach fair, confirm this particulate state sludge acclimatization success, now, the clearance of percolate water outlet COD is 75%, and water outlet COD is down to below 200mg/L,
D, after particulate state sludge acclimatization success, progressively promote the pending percolate load as water inlet, the COD concentration of first stage water inlet is between 1000～1500mg/L, after mensuration water outlet COD drops to below 200mg/L, then inlet COD concentration is increased to 1500～2500mg/L, by that analogy, the water outlet COD of pending percolate all maintains below 200mg/L, hydraulic detention time in this process, temperature is all identical with the particulate state sludge acclimatization stage with the control of stirring.
2. cultivation and the acclimation method of a kind of particulate state mud for the treatment of percolate according to claim 1, it is characterized in that: in steps A, earth choosing method is, push rotted leaf aside, get more moistening soil under rotted leaf, the degree of depth that fetches earth is no more than 5cm, the earth sample of obtaining is packed in an open container, add the distilled water with respect to 5 times of volume left and right of earth sample, stir, there is not larger soil particle, then the suspended substance such as elimination turbid solution upper strata leaf and grass roots, again stir, stop the rear turbid solution that is viscous state that pouring out fast about 2/3 left and right to for subsequent use in another one open container, remaining heavier residue is outwelled.
3. cultivation and the acclimation method of a kind of particulate state mud for the treatment of percolate according to claim 1, it is characterized in that: when in the particulate state culturing sludge of described B step, glucose dosage calculates, the flooding quantity of first day is to calculate with the amount of the middle aged percolate of actual input in reactor.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104843864A (en) * | 2015-06-02 | 2015-08-19 | 广西大学 | Method for improving stability of landfill leachate anaerobic granular sludge |
| CN105217783A (en) * | 2015-11-17 | 2016-01-06 | 梅庆波 | A kind of method of cultivation of anaerobic activated sludge |
| CN108002530A (en) * | 2017-12-22 | 2018-05-08 | 中国海洋大学 | A kind of cultural method for the anaerobic grain sludge for handling the waste water containing polyacrylamide |
| CN113149229A (en) * | 2021-02-24 | 2021-07-23 | 青岛理工大学 | Pure membrane MBBR starting method with soil as inoculation system |
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| JPS62279894A (en) * | 1986-05-30 | 1987-12-04 | Kobe Steel Ltd | Formation of granular sludge in anaerobic treatment process |
| CN101306870A (en) * | 2008-07-07 | 2008-11-19 | 中国科学院成都生物研究所 | Heterotrophic nitrification aerobic granule sludge, culture method thereof and use |
| FR2945039A1 (en) * | 2009-04-30 | 2010-11-05 | Ondeo Ind Solutions | Anaerobic digestion of water from liquid phase containing suspension materials e.g. co-product, comprises subjecting wort to solid/liquid separation phases, and extracting main product from first vegetable material transformed into wort |
| CN102040281A (en) * | 2009-10-21 | 2011-05-04 | 中国石油化工股份有限公司 | Method for culturing denitrogenized granular sludge |
-
2014
- 2014-03-12 CN CN201410090545.6A patent/CN103864226B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62279894A (en) * | 1986-05-30 | 1987-12-04 | Kobe Steel Ltd | Formation of granular sludge in anaerobic treatment process |
| CN101306870A (en) * | 2008-07-07 | 2008-11-19 | 中国科学院成都生物研究所 | Heterotrophic nitrification aerobic granule sludge, culture method thereof and use |
| FR2945039A1 (en) * | 2009-04-30 | 2010-11-05 | Ondeo Ind Solutions | Anaerobic digestion of water from liquid phase containing suspension materials e.g. co-product, comprises subjecting wort to solid/liquid separation phases, and extracting main product from first vegetable material transformed into wort |
| CN102040281A (en) * | 2009-10-21 | 2011-05-04 | 中国石油化工股份有限公司 | Method for culturing denitrogenized granular sludge |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104843864A (en) * | 2015-06-02 | 2015-08-19 | 广西大学 | Method for improving stability of landfill leachate anaerobic granular sludge |
| CN105217783A (en) * | 2015-11-17 | 2016-01-06 | 梅庆波 | A kind of method of cultivation of anaerobic activated sludge |
| CN105217783B (en) * | 2015-11-17 | 2017-05-31 | 陕西惠瑞环保科技工程有限公司 | A kind of method of the culture of anaerobic activated sludge |
| CN108002530A (en) * | 2017-12-22 | 2018-05-08 | 中国海洋大学 | A kind of cultural method for the anaerobic grain sludge for handling the waste water containing polyacrylamide |
| CN113149229A (en) * | 2021-02-24 | 2021-07-23 | 青岛理工大学 | Pure membrane MBBR starting method with soil as inoculation system |
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