CN102702059A - Spirulina beta-renieratene extract and preparation method thereof - Google Patents
Spirulina beta-renieratene extract and preparation method thereof Download PDFInfo
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- CN102702059A CN102702059A CN2012101926234A CN201210192623A CN102702059A CN 102702059 A CN102702059 A CN 102702059A CN 2012101926234 A CN2012101926234 A CN 2012101926234A CN 201210192623 A CN201210192623 A CN 201210192623A CN 102702059 A CN102702059 A CN 102702059A
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Abstract
The invention discloses a method for extracting beta-renieratene with an in-vivo hyperglycemic active function. The method comprises the following steps of: performing ultrasonic-assisted extraction on spirulina powder serving as a raw material, and extracting by optimizing an organic solvent mixing proportion to obtain a crude beta-renieratene extract; and purifying by using a porous adsorption resin to obtain a beta-renieratene extract. Due to the adoption of the method, the yield of beta-renieratene can be increased effectively, and the product has the effects of lowering blood sugar and relieving polydipsia symptom, can be applied to functional foods and nutrient health products, and belongs to the technical fields of modern food processing and biology.
Description
Technical field
The present invention relates to from tenaculat Habenaria, obtain the β-Hu Luobusu preparation method of extract.This extract has hypoglycemic activity, belongs to modern food processing and biological technical field.
Background technology
Tenaculat Habenaria (Spirulina platensis) is recommended as " optimal food of 21 century " by Food and Argriculture OrganizationFAO.This type of algae rich in proteins, polysaccharide, unsaturated fatty acids, nucleic acid and trace element, wherein its carrotenoid (0.2-0.4%) of containing is 10～15 times of other plants that comprise Radix Dauci Sativae.Tenaculat Habenaria can be used as the important source that a large amount high-quality Serlabo is provided.
β-Hu Luobusu belongs to a kind of carrotenoid.It is important food dye and nutrition-fortifying agent at the occurring in nature ubiquity.Confirm that according to multinomial domestic and international research functions such as that natural beta-carotin has is anti-oxidant, antitumor, radioprotective, strengthening immunity can be used as additive and are used for food, industry and agriculture prodn.
About using the auxiliary organic solvent of UW to obtain the still unmanned research of preparation of β-Hu Luobusu extract in the tenaculat Habenaria.Correlative study shows that the impact wave energy that the ultrasonic cavitation effect produces effectively promotes breaking of cell membrane walls to make the effective constituent stripping, and its temperature-rise period can be accelerated the dissolving of β-Hu Luobusu.The β-Hu Luobusu extractive content that at present adopts common extraction method to obtain is low, can not satisfy on the market demand for β-Hu Luobusu.Therefore through optimization production technology, be significant for the tenaculat Habenaria resource that develops China's abundant, research and development functional food etc. thereby improve the extraction yield of β-Hu Luobusu in tenaculat Habenaria.
This patent just is being based on the demand of existing market to β-Hu Luobusu; Through improving, optimize the β-Hu Luobusu that extraction process obtains higher output yield, with this as the important source that obtains the high-quality β-Hu Luobusu from tenaculat Habenaria with provide support for the research and development of relevant healthcare products.
Summary of the invention
The purpose of this invention is to provide tenaculat Habenaria β-Hu Luobusu extract, improve the purposes that extraction process improves productive rate and hypoglycemic activity is provided, and then the tenaculat Habenaria resource of deep development China's abundant.
The tenaculat Habenaria β-Hu Luobusu extract of invention adopts ultrasonic-assisted extraction on existing extraction process basis, optimize organic extraction solvent ratio, solid-to-liquid ratio, extraction time, and confirms suitable means of purification through the purification effect contrast.
For realizing above-mentioned purpose, the present invention includes following steps:
1) first product preparation: the extraction solvent is acetone-carbinol mixture; Acetone: methyl alcohol is 1: 1～2: 1 (v/v), tenaculat Habenaria: extracting solvent is 0.03～0.05 (w/v), and supersonic cell is pulverized under 35～45 ℃ of conditions of temperature; Ultrasonic power is 45～55%, 15～20 minutes treatment times; This method can improve extraction yield.
2) purifying: adopt model NKA-2 macroporous adsorbent resin; Processing parameter: 25～35 ℃ of adsorption temps, last appearance speed 0.5～1.5mL/min; 1: 1～2: 1 (v/v) mixed solvents of desorption solvent acetone methyl alcohol ratio, 25～35 ℃ of desorption temperatures, elution flow rate 0.5～1.5mL/min, eluent is with resolving solvent.
The action thing of going forward side by side is tested, and the result shows that tenaculat Habenaria β-Hu Luobusu extract has the obvious functions of blood sugar activity.
Characteristics of the present invention:
1), can make obviously that β-Hu Luobusu extract output increases by 1～2 times in the tenaculat Habenaria through optimizing ultrasonic technology assisted extraction condition.
2) with the β-Hu Luobusu extract of the inventive method preparation, improve 2～3 times than common extraction method, and the shortening time.
2) the present invention shows that tenaculat Habenaria β-Hu Luobusu extract has hypoglycemic, as to alleviate many diet symptom effect, can be applicable to the exploitation of functional food and nutritive health products.
Description of drawings
The extraction process figure of β-Hu Luobusu in Fig. 1 tenaculat Habenaria.
Fig. 2 model group mouse blood sugar value significant difference.
Respectively organized mouse blood sugar value significant difference after Fig. 3 perfusion on the 10th day.
Respectively organize mouse amount of drinking water significant difference after Fig. 4 perfusion.
Respectively organize mouse food-intake significant difference after Fig. 5 perfusion.
Embodiment
Below in conjunction with accompanying drawing and specific embodiment the present invention is made further detailed description, but do not limit the scope of the invention.
Embodiment
1) extraction of β-Hu Luobusu: get 0.5g exsiccant spirulina powder, handle 17min, 40 ℃ with 12.5mL methyl alcohol-acetone mixing solutions (3: 2) ultra-sonic oscillation under 51% power.
2) purifying of β-Hu Luobusu extract: get 20mL wet resin NKA-2, washing is to there not being the alcohol flavor.Get the ready crude extract of 100mL, supernatant is added in the chromatography column, appearance speed 1.0mL/min in the adjustment waits to slip out liquid and stops to go up appearance near saturated solution.Clean residual impurity in the post with zero(ppm) water.
3) next use the flow velocity desorb of acetone-methanol mixed solution with 1mL/min, every 5min collects cut and uses the UV spectrophotometer measuring concentration of carotene.When effluent reaches 40mL, begin to collect the β-Hu Luobusu extract, until effluent be colourless till.The extract of collecting obtains cryopreservation behind the xln through reduction vaporization.
1) extraction of β-Hu Luobusu: get 10Kg exsiccant spirulina powder, handle 30min, 40 ℃ down in 51% power with 250L methyl alcohol-acetone mixing solutions (3: 2).
2) be used for the resin X5 pre-treatment of industrial purification β-Hu Luobusu extract: after the ethanol with 95% fully soaks swelling, be washed with distilled water to do not have muddy.60 ℃ of vacuum-drying 40～50h.
3) purifying of β-Hu Luobusu extract: get the resin X5 after 40L handles, washing is to there not being the alcohol flavor.Get the ready crude extract of 2000L, supernatant is added in the chromatography column, appearance speed 1.0mL/min in the adjustment waits to slip out liquid and stops to go up appearance near saturated solution.Clean residual impurity in the post with zero(ppm) water.
Next use the flow velocity desorb of acetone-methanol mixed solution with 1mL/min, every 5min collects cut with UV spectrophotometer measuring β-Hu Luobusu extract concentrations.When effluent reaches 800L, begin to collect the β-Hu Luobusu extract, be colourless, not till containing the β-Hu Luobusu extract until effluent.The extract of collecting is preserved through lyophilize.
Embodiment 3:
Extract animal (mouse) determination of activity.Get some of normal mouses and raise after 7 days, the picked at random mouse is divided into normal control group and normal administration group.The fasting of residue mouse is after 12 hours, and (STZ concentration is 30mg/ml to the disposable injection streptozotocin in abdominal cavity (STZ) solution, with the citrate buffer solution preparation, pH4.5) sets up hyperglycemia model, is divided into negative control group and model administration group at random as model mice.It is equal in number that each organizes mouse.
Is that 100mg/kg gives mouse stomach with the CMC suspension liquid of tenaculat Habenaria β-Hu Luobusu extract with the 0.2mL dosage, continuous 10 days survey mouse fasting plasma lipid amounts, body weight, food-intake and amounts of drinking water.
Claims (2)
1. preparation method with β-Hu Luobusu of function of blood sugar reduction in the high body who from tenaculat Habenaria, extracts is characterized in that said method comprising the steps of:
1) first product preparation: the extraction solvent is acetone-carbinol mixture; Acetone: methyl alcohol is 1: 1～2: 1 (v/v), tenaculat Habenaria: extracting solvent is 0.03～0.05 (w/v), and supersonic cell is pulverized under 35～45 ℃ of conditions of temperature; Ultrasonic power is 45～55%, 15～20 minutes treatment times; This method can improve extraction yield;
2) purifying: adopt model NKA-2 macroporous adsorbent resin; Processing parameter: 25～35 ℃ of adsorption temps, last appearance speed 0.5～1.5mL/min; 1: 1～2: 1 (v/v) mixed solvents of desorption solvent acetone methyl alcohol ratio, 25～35 ℃ of desorption temperatures, elution flow rate 0.5～1.5mL/min, eluent is with resolving solvent.
2. a β-Hu Luobusu of making according to claim 1 or 2 said preparing methods is characterized in that said β-Hu Luobusu can be applicable to function of blood sugar reduction food or nutritive health products.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103772255A (en) * | 2014-01-27 | 2014-05-07 | 甘肃凯源生物技术开发中心 | Method for extracting beta-carotene from algae by low-molecule organic matters/inorganic salt aqueous two-phase extraction |
| CN104557648A (en) * | 2014-12-23 | 2015-04-29 | 嘉必优生物工程（武汉）有限公司 | Method for preparing beta-carotene |
| CN104634749A (en) * | 2013-11-12 | 2015-05-20 | 江苏艾兰得营养品有限公司 | Method for determining content of beta-carotene |
| CN106810483A (en) * | 2017-01-22 | 2017-06-09 | 福州大学 | A kind of method of active material in extraction spirulina |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175632A (en) * | 1996-09-03 | 1998-03-11 | 永胜县商业总公司 | Spirulina wine and preparing process thereof |
| CN102175789A (en) * | 2011-01-06 | 2011-09-07 | 天津天狮生物发展有限公司 | HILC (high performance liquid chromatography) measuring method of beta-carotene in spiral seaweed |
-
2012
- 2012-06-13 CN CN201210192623.4A patent/CN102702059B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175632A (en) * | 1996-09-03 | 1998-03-11 | 永胜县商业总公司 | Spirulina wine and preparing process thereof |
| CN102175789A (en) * | 2011-01-06 | 2011-09-07 | 天津天狮生物发展有限公司 | HILC (high performance liquid chromatography) measuring method of beta-carotene in spiral seaweed |
Non-Patent Citations (13)
| Title |
|---|
| 《Chem. Eng. Technol.》 20080527 Mei Huang等 Seperation and Purification of beta-Carotene from Chlorophyll Factory Residues 第922-927页 1-2 第31卷, 第6期 * |
| 《中国公共卫生》 20091231 江咏梅等 镁和beta-胡萝卜素对2型糖尿病大鼠糖脂代谢影响 第1428-1429页 1-2 第25卷, 第12期 * |
| 《工业微生物》 19971231 梁静娟等 螺旋藻beta-胡萝卜素的分离提纯研究 第21-24页 1-2 第27卷, 第2期 * |
| 《现代农村科技》 20120131 房明 螺旋藻beta-胡萝卜素的提取及生物活性研究进展 第70-71页 1-2 , 第1期 * |
| 《色谱》 20010131 胡朝辉等 极大螺旋藻中beta-胡萝卜素的分离纯化及定量测定 第85-87页 1-2 第19卷, 第1期 * |
| 《陕西师范大学硕士学位论文》 20080131 吕爽 胡萝卜素中beta-胡萝卜素的提取、纯化及其稳定性研究 第1-70页 1-2 , * |
| JING LI等: "Development of adsorptive (non-ionic) macroporous resins and their uses in the purification of pharmacologically-active natural products from plant sources", 《NATURAL PRODUCT REPORTS》 * |
| MEI HUANG等: "Seperation and Purification of β-Carotene from Chlorophyll Factory Residues", 《CHEM. ENG. TECHNOL.》 * |
| 吕爽: "胡萝卜素中β-胡萝卜素的提取、纯化及其稳定性研究", 《陕西师范大学硕士学位论文》 * |
| 房明: "螺旋藻β-胡萝卜素的提取及生物活性研究进展", 《现代农村科技》 * |
| 梁静娟等: "螺旋藻β-胡萝卜素的分离提纯研究", 《工业微生物》 * |
| 江咏梅等: "镁和β-胡萝卜素对2型糖尿病大鼠糖脂代谢影响", 《中国公共卫生》 * |
| 胡朝辉等: "极大螺旋藻中β-胡萝卜素的分离纯化及定量测定", 《色谱》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104634749A (en) * | 2013-11-12 | 2015-05-20 | 江苏艾兰得营养品有限公司 | Method for determining content of beta-carotene |
| CN103772255A (en) * | 2014-01-27 | 2014-05-07 | 甘肃凯源生物技术开发中心 | Method for extracting beta-carotene from algae by low-molecule organic matters/inorganic salt aqueous two-phase extraction |
| CN103772255B (en) * | 2014-01-27 | 2016-04-13 | 甘肃凯源生物技术开发中心 | The method of β-carotene in a kind of low molecule organic matter/inorganic salt aqueous two-phase extraction algae |
| CN104557648A (en) * | 2014-12-23 | 2015-04-29 | 嘉必优生物工程（武汉）有限公司 | Method for preparing beta-carotene |
| CN106810483A (en) * | 2017-01-22 | 2017-06-09 | 福州大学 | A kind of method of active material in extraction spirulina |
| CN106810483B (en) * | 2017-01-22 | 2018-07-17 | 福州大学 | A kind of method of active material in extraction spirulina |
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Effective date of registration: 20200319 Address after: 016100 the Inner Mongolia Autonomous Region city Etuokeqi Erdos Wulan Town Industrial Park of Spirulina Patentee after: ORDOS XINYULI SPIRULINA INDUSTRY GROUP Co.,Ltd. Address before: 100083 Haidian District Qinghua East Road, No. 35, Beijing Patentee before: BEIJING FORESTRY University |