CN102702059B - Spirulina beta-renieratene extract and preparation method thereof - Google Patents
Spirulina beta-renieratene extract and preparation method thereof Download PDFInfo
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- CN102702059B CN102702059B CN201210192623.4A CN201210192623A CN102702059B CN 102702059 B CN102702059 B CN 102702059B CN 201210192623 A CN201210192623 A CN 201210192623A CN 102702059 B CN102702059 B CN 102702059B
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Abstract
The invention discloses a method for extracting beta-renieratene with an in-vivo hyperglycemic active function. The method comprises the following steps of: performing ultrasonic-assisted extraction on spirulina powder serving as a raw material, and extracting by optimizing an organic solvent mixing proportion to obtain a crude beta-renieratene extract; and purifying by using a porous adsorption resin to obtain a beta-renieratene extract. Due to the adoption of the method, the yield of beta-renieratene can be increased effectively, and the product has the effects of lowering blood sugar and relieving polydipsia symptom, can be applied to functional foods and nutrient health products, and belongs to the technical fields of modern food processing and biology.
Description
Technical field
The present invention relates to obtain the preparation method of β-carotene extract from spirulina.This extract has hypoglycemic activity, belongs to modern food processing and biological technical field.
Background technology
Spirulina (Spirulina platensis) united state food and agricultural organization is recommended as " optimal food of 21 century ".This type of algae rich in proteins, polysaccharide, unsaturated fatty acids, nucleic acid and trace element, the carotenoid (0.2-0.4%) that wherein it contains is 10～15 times of other plants that comprise Radix Dauci Sativae.Spirulina can be used as the important sources that a large amount high-quality carotene is provided.
β-carotene belongs to a Carotenoids.It is important food dye and nutrition-fortifying agent at the occurring in nature ubiquity.According to multinomial domestic and international research, confirm, the functions such as that natural beta-carotin has is anti-oxidant, antitumor, radioprotective, strengthening immunity, can be used as additive for food, industry and agriculture production.
There is no people's research about the preparation of using the auxiliary organic solvent of ultrasonic wave to obtain β-carotene extract in spirulina.Correlative study shows, the impact wave energy that the ultrasonic cavitation effect produces effectively promotes breaking of cell membrane walls to make the effective constituent stripping, and its temperature-rise period can be accelerated the dissolving of β-carotene.The β-carotene extractive content that at present adopts common extraction method to obtain is low, can not satisfying the market on for the demand of β-carotene.Therefore by optimized production process, thereby improve the extraction yield of β-carotene in spirulina, for the spirulina resource that develops China's abundant, research and development functional food etc., be significant.
This patent just is being based on the demand of existing market to β-carotene, by the β-carotene improved, Optimized Extraction Process obtains higher output yield, take that this provides support as the important sources that obtains the high-quality β-carotene from spirulina with for the research and development of the healthcare products of being correlated with.
Summary of the invention
The purpose of this invention is to provide spirulina β-carotene extract, improve the purposes that extraction process improves productive rate and hypoglycemic activity is provided, and then the spirulina resource of deep development China's abundant.
The spirulina β-carotene extract of invention, on existing extraction process basis, adopts ultrasonic-assisted extraction, optimizes organic extraction solvent ratio, solid-to-liquid ratio, extraction time, and contrasts and determine suitable means of purification by purification effect.
For achieving the above object, the present invention includes following steps:
1) first product preparation: the extraction solvent is acetone-carbinol mixture, acetone: methyl alcohol is 1: 1～2: 1 (v/v), spirulina: extracting solvent is 0.03～0.05 (w/v), under 35～45 ℃ of conditions of temperature, supersonic cell is pulverized, ultrasonic power is 45～55%, 15～20 minutes treatment times; This method can improve extraction yield.
2) purifying: adopt model NKA-2 macroporous adsorbent resin; Processing parameter: 25～35 ℃ of adsorption temps, loading speed 0.5～1.5mL/min; 1: 1～2: 1 (v/v) mixed solvents of desorption solvent acetone methyl alcohol ratio, 25～35 ℃ of desorption temperatures, elution flow rate 0.5～1.5mL/min, eluent is with resolving solvent.
The action thing of going forward side by side is tested, and result shows that spirulina β-carotene extract has obvious hypoglycemic activity.
Characteristics of the present invention:
1), by Optimization for Ultrasonic Wave technology assisted extraction condition, can obviously make β-carotene extract output in spirulina increase by 1～2 times.
2) the β-carotene extract prepared by the inventive method, improve 2～3 times than common extraction method, and shorten the time.
2) the present invention shows that spirulina β-carotene extract has hypoglycemic, as to alleviate many diet symptom effect, can be applicable to functional food and nutritive health products exploitation.
The accompanying drawing explanation
The extraction process figure of β-carotene in Fig. 1 spirulina.
Fig. 2 model group mouse blood sugar value significant difference.
Within after Fig. 3 perfusion the 10th day, respectively organize mouse blood sugar value significant difference.
Respectively organize mouse amount of drinking water significant difference after Fig. 4 perfusion.
Respectively organize mouse food-intake significant difference after Fig. 5 perfusion.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail, but do not limit the scope of the invention.
Embodiment
1) extraction of β-carotene: get the spirulina powder of 0.5g drying, with 12.5mL methanol-acetone mixing solutions (3: 2) ultra-sonic oscillation under 51% power, process 17min, 40 ℃.
2) purifying of β-carotene extract: get 20mL wet resin NKA-2, be washed to without the alcohol flavor.Get the ready crude extract of 100mL, supernatant liquor is added in chromatography column, adjust loading speed 1.0mL/min, liquid to be slipped out approaches saturated solution and stops loading.Clean residual impurity in post with distilled water.
3) next use the flow velocity desorb of acetone-methanol mixed solution with 1mL/min, every 5min collects cut UV spectrophotometer measuring concentration of carotene.When reaching 40mL, effluent liquid starts to collect the β-carotene extract, until effluent liquid is colourless.Extract cryopreservation after reduction vaporization obtains xln of collecting.
1) extraction of β-carotene: get the spirulina powder of 10Kg drying, with 250L methanol-acetone mixing solutions (3: 2), process 30min under 51% power, 40 ℃.
2) for the resin X5 pre-treatment of industrial purification β-carotene extract: after the ethanol with 95% fully soaks swelling, be washed with distilled water to without muddy.60 ℃ of vacuum-drying 40～50h.
3) purifying of β-carotene extract: get the resin X5 after 40L processes, be washed to without the alcohol flavor.Get the ready crude extract of 2000L, supernatant liquor is added in chromatography column, adjust loading speed 1.0mL/min, liquid to be slipped out approaches saturated solution and stops loading.Clean residual impurity in post with distilled water.
Next use the flow velocity desorb of acetone-methanol mixed solution with 1mL/min, every 5min collects UV spectrophotometer measuring β-carotene extract concentrations for cut.When reaching 800L, effluent liquid starts to collect the β-carotene extract, until effluent liquid is colourless, not till containing the β-carotene extract.The extract of collecting is preserved through lyophilize.
Embodiment 3:
Extract animal (mouse) determination of activity.Get some of normal mouses and raise after 7 days, choose at random mouse and be divided into Normal group and normal administration group.The fasting of residue mouse is after 12 hours, (STZ concentration is 30mg/ml to the disposable injection streptozotocin in abdominal cavity (STZ) solution, with the citrate buffer solution preparation, pH4.5) set up hyperglycemia model, be divided at random negative control group and model administration group as model mice.It is equal in number that each organizes mouse.
By the CMC suspension liquid of spirulina β-carotene extract take the 0.2mL dosage as 100mg/kg to mouse stomach, within continuous 10 days, survey mouse fasting plasma lipid amount, body weight, food-intake and amount of drinking water.
Claims (1)
1. a method of extracting β-carotene from spirulina is characterized in that:
1) extraction of β-carotene: get the spirulina powder of 0.5g drying, with 12.5ml methanol-acetone mixing solutions, the volume ratio of methyl alcohol and acetone is 3: 2, and under 51% power, ultra-sonic oscillation are processed 17min, 40 ℃;
2) β-carotene extract purifying: get 20ml wet resin NKA-2, be washed to without the alcohol flavor, get the ready crude extract of 100ml, supernatant liquor is added in chromatography column, adjust loading speed 1.0ml/min, treat that effluent liquid approaches saturated solution and stops loading, with distilled water, clean residual impurity in post;
3) next with acetone-methanol mixed solution, with the flow velocity of 1ml/min, start desorb, within every 5 minutes, collect cut UV spectrophotometer measuring concentration of carotene, when reaching 40ml, effluent liquid starts to collect the β-carotene extract, until effluent liquid is colourless, the extract of collection cryopreservation after reduction vaporization obtains xln.
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| CN104634749A (en) * | 2013-11-12 | 2015-05-20 | 江苏艾兰得营养品有限公司 | Method for determining content of beta-carotene |
| CN103772255B (en) * | 2014-01-27 | 2016-04-13 | 甘肃凯源生物技术开发中心 | The method of β-carotene in a kind of low molecule organic matter/inorganic salt aqueous two-phase extraction algae |
| CN104557648A (en) * | 2014-12-23 | 2015-04-29 | 嘉必优生物工程（武汉）有限公司 | Method for preparing beta-carotene |
| CN106810483B (en) * | 2017-01-22 | 2018-07-17 | 福州大学 | A kind of method of active material in extraction spirulina |
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| CN102175789A (en) * | 2011-01-06 | 2011-09-07 | 天津天狮生物发展有限公司 | HILC (high performance liquid chromatography) measuring method of beta-carotene in spiral seaweed |
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Effective date of registration: 20200319 Address after: 016100 the Inner Mongolia Autonomous Region city Etuokeqi Erdos Wulan Town Industrial Park of Spirulina Patentee after: ORDOS XINYULI SPIRULINA INDUSTRY GROUP Co.,Ltd. Address before: 100083 Haidian District Qinghua East Road, No. 35, Beijing Patentee before: BEIJING FORESTRY University |