CN102698096B - Composition of traditional Chinese medicine extracts and preparation method and application thereof - Google Patents
Composition of traditional Chinese medicine extracts and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a composition of traditional Chinese medicine extracts for treating diabetes, a preparation method for the composition and the application of corn silk polysccaharides and crude baicalin in preparing a medicine used for decreasing blood sugar. The corn silk polysccaharides and the crude baicalin disclosed by the invention can be obtained respectively by isolating, extracting and purifying corn silk and Scutellaria baicalensis by utilizing a simple and effective extraction process and method. According to pharmacology experiments, the composition of the traditional Chinese medicine extracts is obvious in activity of decreasing the in-vivo blood sugar.
Description
Technical field
The present invention relates to the compositions of a Plant Extracts, more particularly, a kind of corn silk polysaccharide and a kind of thick baicalin that extraction obtains from Radix Scutellariae (Scutellaria baicalensis Georgi) and preparation method thereof that obtain and said composition extracted from Stigma Maydis (Zeamays L.) is related to for the preparation of the purposes for the treatment of in hypoglycemic medicine.
Background technology
Diabetes are endocrine metabolism diseases that a kind of cause of disease is very complicated, and its basic pathology physiology is the hypoinsulinism of absoluteness or relative property; And β cell reduces the sensitivity of insulin, thus cause the water and electrolyte metabolism of sugar, protein, fat and secondary disorderly.Acid base imbalance can be caused time serious.Its typical characteristics is: hyperglycemia, glucose in urine, glucose tolerance reduction and insulin release test are abnormal.Clinical manifestation is polydipsia, polyphagia, polyuria, excessive thirst, easy famine, become thin, the symptoms such as fatigue and weak, diabetics is due to inherited genetic factors, environmental factors, life factor, Nervous and Mental Factors, cause immune function of human body obstacle, interleukin reactivity is caused to increase, promote islet cells endolymph cellular infiltration, the cell infiltrated produces a kind of antagonism insulin function infringement factor lymph cytokines, lymph cytokines hinders beta Cell of islet blood circulation, kill and wound beta Cell of islet and sugar carrier gene, destroy insulin secretion, the links of excretion and transhipment, cause the infringement of islet function, cause diabetes and diabetic complication.Type 2 diabetes mellitus, is called non-insulin-dependent diabetes mellitus more, and sickness rate accounts for 90% of diabetic sum; Age of onset is most more than 35 years old.
Stigma Maydis, Radix Scutellariae have the curative effect for the treatment of diabetes and complication preferably respectively.
Stigma Maydis is style and the stigma of grass Semen Maydis Zea mays L..Medicinal record the earliest sees 1476 " the southern regions of the Yunnan Province wood grass ", and be the traditional Chinese crude drug of China, " Chinese Pharmacopoeia " records for 1977.For " Ministry of Health of the People's Republic of China's medical material standard " 1985 editions medicinal herbs most in use kinds that () includes.Stigma Maydis all has diuresis to people or rabbit, can increase chloride output, and the insoluble part of its aqueous extract methanol is the strongest through dialysis person's diuresis, and no matter oral, subcutaneous or intravenous injection all has remarkable result.Corn stigma can promote bile excretion, reduces its viscosity, reduces its bile pigments content, thus can be used as the cholangitis patient of choleretic for uncomplicated chronic cholecystitis, bile discharge obstacle.It can also accelerate Blood Coagulation Process, increases prothrombing content in blood, therefore can be used as hemorrhage diuretic of holding concurrently and be applied to bladder and lithangiuria.Once studies have reported that about the hypoglycemic activity of corn silk polysaccharide to lumbar injection alloxan modeling mice.
Baicalin (Baicailin, Bai) is a kind of flavone compound extracted from Chinese crude drug Radix Scutellariae (Scutellariabaicalensis georgi) root, has pharmacological action widely.Research finds the effect that baicalin has good anti peroxidation of lipid, suppresses aldose reductase (AR), prevents and treats the chronic complicating diseases such as diabetic peripheral neuropathy and diabetic nephropathy.
Summary of the invention
The present invention utilizes simple and effective extraction process and method, is that raw material obtains a kind of corn silk polysaccharide with Stigma Maydis; Be that raw material obtains a kind of thick baicalin with Radix Scutellariae; By corn silk polysaccharide and thick baicalin 1: 1 mix homogeneously by weight, the compound preparation (compositions of namely described Chinese medicine extract) that the present invention treats diabetes can be obtained.Pharmacological evaluation shows that this compound preparation has and regulates blood glucose function and corresponding hypoglycemic activity.Therefore this compound preparation is expected to exploitation becomes a kind of as hypoglycemic health care product or medicine or assist a ruler in governing a country medicine and for clinical.
Therefore, one object of the present invention be to provide that a kind of molecular weight ranges, sugar composition are clear and definite, chemical composition and prescription weight ratio clearly compound preparation.
Another object of the present invention is to provide a kind of preparation method of compositions of Chinese medicine extract.
Another object of the present invention is to provide a kind of compositions being used for the treatment of hypoglycemic Chinese medicine extract, it comprises 40-4, the compositions of the Chinese medicine extract in 000mg/kg/d effective dosage ranges is as effective ingredient, and preferably, it can contain pharmaceutically acceptable carrier and adjuvant further.
The compositions that another object of the present invention is to provide Chinese medicine extract is for the preparation of the purposes in the hypoglycemic medicine for the treatment of.
In the present invention, the sugar of described corn silk polysaccharide consists of rhamnose, arabinose, xylose, mannose, galactose and glucose.Its mol ratio is: rhamnose (Rha): arabinose (Ara): xylose (Xyl): mannose (Man): galactose (Gla): glucose (Glu)=0.25: 0.86: 0.81: 0.47: 0.84: 1.22; Mean molecule quantity is 42,095Da, and molecular weight ranges is 1,500-100,000Da.
In the present invention, described thick baicalin is pale yellow powder, dissolves in the alkali liquor such as sodium bicarbonate, sodium carbonate and sodium hydroxide.
According to another object of the present invention, the invention provides the method for these two kinds of Chinese medicine extract of preparation.
(1) from the style and stigma of grass Semen Maydis Zea mays L., carry out separation and Extraction and purification, comprise the steps:
(1) get dry Stigma Maydis medical material, the soak with ethanol defat (merceration ethanol did not have medical material) with 95%, changes three times, soaks complete, gained solids is placed in room temperature ventilation and dries, then extract.1kg raw material adds 10L tap water heating at every turn and boils, and extraction time is 2 ~ 3 hours, and with sugared content in phend-sulphuric acid Detection and Extraction liquid, not obvious to sugar reaction, amount to extraction 6 times; Concentrated extracting solution, cooling.Add equal-volume 30% (W/V) solution of trichloroacetic acid, make its final concentration be 15% (W/V);
Being neutralized to pH with 10%NaOH after (2) 4 DEG C of deproteinization is 7.0 to 8.0, centrifugal.Supernatant is dialysed two days to circulating water, and dialysis solution is concentrated into 2-3L, adds 3 times amount 95% alcohol settling, leaves standstill, pelleting centrifugation.By absolute ethanol washing post-drying for several times, obtain corn silk polysaccharide;
Wherein, the alcoholic solution described in step (1) is 95% ethanol, and it is with 95% soak with ethanol defat three times that ground alcoholic solution soaks.
The condition of the boiling water extraction described in step (1) is: add about 10L distilled water at every turn, and extraction time is 2 ~ 3 hours, and with sugared content in phend-sulphuric acid Detection and Extraction liquid, extracts 3 ~ 5 times, preferably extracts 6 times.
Dialysis condition described in step (1) is: adopt and dialyse 2 days to circulating water.
(2) from labiate Radix Scutellariae (Scutellaria baicalensis Georgi) root, carry out separation and Extraction and purification, comprise the steps:
Radix scutellariae medicinal materials is broken into coarse powder, after soaking 5 hours with the alcohol of 8 times amount, in 90 DEG C of reflux, extract, 4 times, each 2 hours (material, liquid volume ratios: 1: 2, namely the radix scutellariae medicinal materials coarse powder in reflux, extract, and the volume ratio of extracting solution are 1: 2), obtain filtrate after filtration, reclaim ethanol extremely without alcohol taste, obtain aqueous solution, aqueous solution is heated to 80 DEG C, and hydrochloric acid is adjusted to pH2, after insulation 0.5h, continue to leave standstill 20h, centrifugal, obtain precipitation, be washed till with acetone colourless, 60 DEG C of dryings, obtain thick baicalin (yield: 7.9%); Wherein, described alcoholic solution is 60% ethanol.
In a preferred embodiment of the invention, prepare corn silk polysaccharide to comprise the steps:
(1) get dry Stigma Maydis medical material, the soak with ethanol defat (merceration ethanol did not have medical material) with 95%, changes three times, soaks complete, gained solids is placed in room temperature ventilation and dries; Then extract, 1kg raw material adds 10L tap water heating at every turn and boils, and extraction time is 2 ~ 3 hours, and with sugared content in phend-sulphuric acid Detection and Extraction liquid, not obvious to sugar reaction, amount to extraction 6 times; Concentrated extracting solution, cooling.Add equal-volume 30% (W/V) solution of trichloroacetic acid, make its final concentration be 15% (W/V), 4 DEG C of deproteinization, being neutralized to pH with 10%NaOH is afterwards 7.0 to 8.0, centrifugal.Supernatant is dialysed two days to circulating water, and dialysis solution is concentrated into 2-3L, adds 3 times amount 95% alcohol settling, leaves standstill, pelleting centrifugation.By absolute ethanol washing post-drying for several times, obtain corn silk polysaccharide (yield: 4.2%).
According to another object of the present invention, the invention provides one and be used for the treatment of hypoglycemic pharmaceutical composition, it comprises 40-4, and the compositions of 000mg/kg/d effective dosage ranges is as effective ingredient, and preferably, it can contain pharmaceutically acceptable carrier and adjuvant further.
Compositions of the present invention can oral administration administration.During oral administration administration, directly or first this compounds can be made to mix with conventional medicinal adjuvant (as excipient, disintegrating agent, adhesive, lubricant, antioxidant, coating materials, coloring agent, aromatic, surfactant etc.), be made into the forms such as granule, capsule, tablet.When preparing above-mentioned preparation, conventional preparation technique can be used.
According to another object of the present invention, the invention provides said composition for the preparation for the treatment of hypoglycemic medicine in purposes.The present invention utilizes simple and effective extraction process and method, with Stigma Maydis and Radix Scutellariae for raw material obtains corn silk polysaccharide and thick baicalin, and adopts the formula of weight ratio 1: 1 to form.Pharmacological evaluation shows that said composition has blood glucose regulation function and corresponding hypoglycemic activity.Therefore said composition is expected to exploitation becomes a kind of as hypoglycemic health care product or medicine or assist a ruler in governing a country medicine for clinical.
Accompanying drawing explanation
Fig. 1 is the extraction separation and purification flow chart of the corn silk polysaccharide according to an embodiment of the invention.
Fig. 2 is the extraction separation and purification flow chart of the thick baicalin according to an embodiment of the invention
Fig. 3 is infrared spectrum (IR) figure of the corn silk polysaccharide according to an embodiment of the invention.
Fig. 4 is infrared spectrum (IR) figure according to the thick baicalin of an embodiment of the invention.
Fig. 5 is sugared composition analysis figure (GC) figure of the corn silk polysaccharide according to an embodiment of the invention.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further elaborated, and following embodiment only describes the present invention by way of example.Clearly, those of ordinary skill in the art in scope of the present invention and essence, can carry out various accommodation and amendment to the present invention.It is to be understood that this invention is intended to contain the accommodation and amendment that comprise in the dependent claims.
Instrument:
Shimadzu SIL-20A automatic sampler, LC-20AD high-pressure pump, CTO-20A column oven, SPD-20A ultraviolet-visible detector, CBM-20A system controller and GPC data processing software.(7.8mm × 300mm, hydroxylated polymethyl methacrylate, exclusion limit is respectively 4 × 10 to two polysaccharide special gel chromatographic column Ultrahydrogel 500 and Ultrahydrogel 2000
5with 7 × 10
6and the series connection of pre-column Ultrahydrogel Guard Column (6 μm, 6x40mm) Da); Chromatographic condition: for mobile phase, column temperature is 35 DEG C with Buffer (7.32g dipotassium hydrogen phosphate and 0.4g potassium dihydrogen phosphate mix and be settled to 1L), and flow velocity is 0.5ml per minute, and sample size is 20 μ l.
The automatic fraction collector of BSZ-100 and HL-2 constant flow pump (Qingpu Shanghai Hu Xi instrument plant), Labconco6 type freeze dryer (Labconco company of Britain), Hitachi CR-22G type high speed centrifuge (Japanese Hitachi company), electric heating constant-temperature blowing drying box (the upper grand experimental facilities company limited of Nereid), EYELA-N1100 type Rotary Evaporators, UV1000 ultraviolet-uisible spectrophotometer (Shanghai Techcomp Instrument Ltd.).
Medicine and reagent:
Stigma Maydis entity is purchased from Hu Qingyu Pharmaceutical Workshop, Shanghai pharmacy.
Radix Scutellariae entity is purchased from Hu Qingyu Pharmaceutical Workshop, Shanghai pharmacy.
Bag filter (molecular retention is 3,500Da), other reagent sources are in Shanghai traditional Chinese medicines group.
The preparation of embodiment 1 corn silk polysaccharide:
Get Stigma Maydis (purchased from Hu Qingyu Pharmaceutical Workshop, Shanghai pharmacy) 1 kilogram, the soak with ethanol with 95% 3 times, dries after terminating.Dry product boiling water extraction after defat, adds water about 10 liters at every turn, and extraction time is 2-3 hour, with the sugared content of Phenol sulfuric acid procedure Detection and Extraction liquid, until sugar reaction is not obvious, amounts to extraction 7 times.Each extracting solution is merged, is finally concentrated into about 2L, adds equal-volume 30% (W/V) solution of trichloroacetic acid, make its final concentration be 15% (W/V), in 4 DEG C of deproteinization 3h, adjust pH to neutral with 10%NaOH afterwards, 12000rpm is centrifugal, gets supernatant, and supernatant is dialysed two days to circulating water, dialysis solution is concentrated into about 2L, add 3 times amount 95% alcohol settling, hold over night, 12,000rpm is centrifugal, gets precipitation.After dehydrated alcohol and acetone wash successively, in 50 DEG C of heat dryings, obtain corn silk polysaccharide, sepia solid, as shown in Figure 1, as shown in Figure 3, its sugared composition analysis figure (GC) figure as shown in Figure 5 for infrared spectrum (IR) figure of corn silk polysaccharide for its separation and purification flow chart.
The preparation of the thick baicalin of embodiment 2:
Radix scutellariae medicinal materials 50 DEG C of dryings are broken into coarse powder, crosses 60 order medicines sieves, by 60% soak with ethanol 5 hours of 8 times amount, in 90 DEG C of reflux, extract, 4 times.Each 2 hours, material, liquid volume ratio were 1: 2, obtained filtrate after filtration, and filtrate recycling ethanol, to without alcohol taste, obtains aqueous solution.Aqueous solution is heated to 80 DEG C, and the hydrochloric acid with 37.5% is adjusted to aqueous solution pH2, after 80 DEG C of insulation 0.5h, continues to leave standstill 20h, centrifugal, obtains precipitation.Precipitation acetone is washed till colourless, 60 DEG C of dryings.(yield: 7.9%), as shown in Figure 2, infrared spectrum (IR) figure of this thick baicalin as shown in Figure 4 for its separation and purification flow chart to obtain thick baicalin.
EXPERIMENTAL EXAMPLE
Experimental example 1 pharmacological evaluation:
1, the compositions (S1) of this Chinese medicine extract is to type 2 diabetes mellitus mice blood sugar reducing function
The principal character of type 2 diabetes mellitus be insulin resistant and insulin secretion definitely or relative deficiency, and cause glycoprotein and fat metabolic disturbance thus, from natural plants, find effective blood sugar reducing component may provide new method for treatment diabetes.
1.1 experiment material
A () raw material: S1, brown dry powder, is provided by Saccharidia Bio-Pharmaceutical Technology (Shanghai) Co., Ltd., and-20 degree are preserved.
(b) experiment mice: KK-A
ymice is a kind of coat color gene (ay) saltant type diabetic mice, ay gene not only affects the hair color of mice but also can cause metabolism disorder, there is the Metabolic Syndrome such as obesity, hyperglycemia, lipid metabolic disorder and hyperinsulinemia, its morbidity on the basis of inheritance susceptible, adds environmental factors and brings out, show very similar to mankind's type 2 diabetes mellitus, being a kind of desirable mankind's patients with type Ⅰ DM animal model, is the typical model of insulin resistant.
KK-A
ydiabetic mice 32, male, body weight 26 ± 1g, purchased from Beijing HFK Bio-Technology Co., Ltd., all raises with high lipid food (being provided by Beijing HFK Bio-Technology Co., Ltd.), feed with sterilizing plastics mouse cage, freely drink water, Animal House temperature controls at 20-25 degree, humidity 55-65%, illumination 10-12h/d, mouse cage is changed 2-3 time for one week.
(c) experimental apparatus: blood glucose meter, the remarkable type blood glucose meter of Roche (
performa), Roche blood sugar test paper
1.2 experimental technique
A () is divided into high, medium and low 3 dosage groups and matched group at random, respectively with isopyknic 100,300, the continuous gavage of S1 of 600mg/kg/d 8 weeks.
B () measures mice fasting glucose 1 time in every two weeks, fasting 3hr before measuring, cuts tail and get blood.
Table 1. test dose grouped table
| Group | Compound | Animals | Route | Dose(mg/kg) | Schedule | Model |
| 1 | Control1 | 8 | po | 0.1ml/10g，BW | Qd＊56 | KK-A y |
| 2 | S1 | 8 | po | 100mg/kg | Qd＊56 | KK-A y |
| 3 | S1 | 8 | po | 300mg/kg | Qd＊56 | KK-A y |
| 4 | S1 | 8 | po | 600mg/kg | Qd＊56 | KK-A y |
Each group of fasting blood sugar tested by table 2
Table 2 shows, when testing beginning, each treated animal fasting glucose is without significant difference; Gavage is after 2 weeks, when the fasting glucose of S1 100mg/kg and 600mg/kg administration group comparatively starts and concurrent control group significantly reduce (equal p < 0.05); After several all administrations, blood glucose value follows contrast ratio again without significant difference afterwards.
Experimental example 2 pharmacological evaluation:
The impact of 1.S1 on diabetic mice OGTT and body weight is tested
1.1 experiment material
A () raw material: S1, brown dry powder, is provided by Saccharidia Bio-Pharmaceutical Technology (Shanghai) Co., Ltd., and-20 degree are preserved.
(b) experiment mice: KK-A
ydiabetic mice 32, male, body weight 26 ± 1g, purchased from Beijing HFK Bio-Technology Co., Ltd., all raises with high lipid food (being provided by Beijing HFK Bio-Technology Co., Ltd.), feed with sterilizing plastics mouse cage, freely drink water, Animal House temperature controls at 20-25 degree, humidity 55-65%, illumination 10-12h/d, mouse cage is changed 2-3 time for one week.
(c) experimental apparatus: blood glucose meter, the remarkable type blood glucose meter of Roche (
performa), Roche blood sugar test paper
1.2 experimental technique
Respectively organize mice time (a) the 4th week and do carbohydrate tolerance experiment (oral glucose tolerance test, OGTT).Before OGTT, mice 3h on an empty stomach, can freely drink water.OGTT same day, glucose is dissolved in sterile purified water respectively by 2g/kg body weight, each group give medicine respectively after 10min to Glucose Liquid, gavage liquid capacity amount 0.1ml/10g Mouse Weight, the change of blood sugar of 30min, 1h, 2h when observing gavage and after gavage.
B () tests each group when administration surrounding, give 2g/kg glucose on an empty stomach, detects to the change of blood sugar after sugar when 30min, 60min and 120min.Fasting 3hr before measuring, cuts tail and gets blood.
Each group of OGTT value tested by table 3
Table 3 shows, and the OGTT result of gavage after 4 weeks has no each group of blood glucose value and matched group, and there were significant differences.
C () tests each group of successive administration eight weeks, within every two weeks, measure body weight once, observes medicine to the impact of each experimental group body weight.
Table 4 each experimental group body weight change table
Table 4 shows, when 2 weeks, S1 100mg/kg administration group body weight has the trend (p < 0.05) alleviated compared with matched group.But body weight starts again to recover after a few week of administration afterwards, have no that other are xicity related.
KK-A
ydiabetic mice is in the process of feeding S1, and each gavage liquid capacity amount (ml) is 0.01 times of Mouse Weight (g), and after 6 weeks, mice does not occur that digestive function reduces phenomenon, and body weight and matched group are without significant difference.
Experimental example 3 pharmacological evaluation:
1.S1 tests the impact of post-prandial glycemia
1.1 experiment material
A () raw material: S1, brown dry powder, is provided by Saccharidia Bio-Pharmaceutical Technology (Shanghai) Co., Ltd., and-20 degree are preserved.
(b) experiment mice: KK-A
ydiabetic mice 32, male, body weight 26 ± 1g, purchased from Beijing HFK Bio-Technology Co., Ltd., all raises with high lipid food (being provided by Beijing HFK Bio-Technology Co., Ltd.), feed with sterilizing plastics mouse cage, freely drink water, Animal House temperature controls at 20-25 degree, humidity 55-65%, illumination 10-12h/d, mouse cage is changed 2-3 time for one week.
(c) experimental apparatus: blood glucose meter, the remarkable type blood glucose meter of Roche (
performa), Roche blood sugar test paper
D () be 1 method empirically, in administration after 8 weeks, test each group under non-fasting state, give drug treating, the change of blood sugar respectively after measurement administration after 0min, 30min, 60min, 120min.
The each experimental group administration of table 5 is postprandial plasma glucose level after 8 weeks
The each experimental group administration of table 6. is postprandial plasma glucose level after 9 weeks
The each experimental group administration of table 7. is postprandial plasma glucose level after 10 weeks
Table 5 shows, in administration after 8 weeks, tests each group under non-fasting state, gives drug treating, the change of blood sugar respectively after measurement administration after 0min, 30min, 60min and 120min.There were significant differences (p < 0.05) for the blood glucose value of S1 600mg/kg group when 120min and matched group.
Claims (7)
1. treat a compositions for the Chinese medicine extract of diabetes, it is characterized in that: the composition of said composition is: corn silk polysaccharide and thick baicalin, the weight ratio of described corn silk polysaccharide and thick baicalin is 1: 1.
2. the compositions of Chinese medicine extract according to claim 1, is characterized in that: the sugar of described corn silk polysaccharide consists of: rhamnose, arabinose, xylose, mannose, galactose and glucose; Its mol ratio is rhamnose: arabinose: xylose: mannose: galactose: glucose=0.25: 0.86: 0.81: 0.47: 0.84: 1.22; Molecular weight ranges is 1,500-100,000Da, and mean molecule quantity is 42,095Da.
3. the compositions of Chinese medicine extract according to claim 1, is characterized in that: the dosage form of the compositions of described Chinese medicine extract is capsule.
4. a preparation method for the compositions of Chinese medicine extract according to claim 1, is characterized in that: described preparation method comprises: (1) is that raw material extracts corn silk polysaccharide with Stigma Maydis; (2) be that raw material extracts thick baicalin with Radix Scutellariae; And by corn silk polysaccharide and thick baicalin by weight 1: 1 mix homogeneously, obtain the compositions of described Chinese medicine extract (3).
5. the preparation method of the compositions of Chinese medicine extract according to claim 4, it is characterized in that, the extracting method of described corn silk polysaccharide comprises following concrete steps: get dry Stigma Maydis medical material, with the soak with ethanol defat of 95%, merceration ethanol did not have medical material, change three times, soak complete, gained solids after defat is placed in room temperature ventilation dry, then extract, 1kg raw material adds 10L tap water heating at every turn and boils, extraction time is 2 ~ 3 hours, and with sugared content in phend-sulphuric acid Detection and Extraction liquid, not obvious to sugar reaction, amount to extraction 6 times, concentrated extracting solution, cooling, add isopyknic 30% solution of trichloroacetic acid, its final concentration is made to be 15%, at 4 DEG C, deproteinization was neutralized to pH with 10%NaOH after 3 hours is 7.0 to 8.0, centrifugal, supernatant is dialysed two days to circulating water, dialysis solution is concentrated into 2-3L, add 3 times amount 95% alcohol settling, leave standstill, pelleting centrifugation, with absolute ethanol washing rear 50 DEG C of oven dry for several times, obtain corn silk polysaccharide, extraction ratio is 4.2%.
6. the preparation method of the compositions of Chinese medicine extract according to claim 4, it is characterized in that: the extracting method of described thick baicalin comprises following concrete steps: radix scutellariae medicinal materials is broken into coarse powder, by 60% soak with ethanol of 8 times amount after 5 hours, in 90 DEG C of reflux, extract, 4 times, each 2 hours, radix scutellariae medicinal materials coarse powder in reflux, extract, and the volume ratio of extracting solution are 1: 2, filtrate is obtained after filtration, reclaim ethanol extremely without alcohol taste, obtain aqueous solution, aqueous solution is heated to 80 DEG C, hydrochloric acid is adjusted to pH2, after insulation 0.5h, continue to leave standstill 20h, centrifugal, must precipitate, be washed till colourless with acetone, 60 DEG C of dryings, obtain thick baicalin.
7. the purposes of compositions in the hypoglycemic medicine of preparation treatment of a Chinese medicine extract according to claim 1, it is characterized in that: the composition of described compositions is corn silk polysaccharide and thick baicalin, and the weight ratio of described corn silk polysaccharide and thick baicalin is 1: 1; Said composition is as hypoglycemic drug use, and its compositions comprising in 40-4000mg/kg/d effective dosage ranges, described Chinese medicine extract as effective ingredient, and contains the carrier and adjuvant that pharmaceutically can accept.
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| 玉米须多糖对糖尿病小鼠降糖作用的研究;俞利平等;《浙江中医杂志》;20090416(第04期);第258-259页 * |
| 玉米须多糖治疗糖尿病作用机制的研究;刘娟等;《中药新药与临床药理》;20060830(第04期);第242-244页 * |
| 玉米须多糖的降血糖作用研究;杨灵玲等;《食品科技》;20110331(第03期);第152-154页 * |
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