CN104013669A - Method for preparing cyclocarya paliurus total flavonoid with effect of reducing blood glucose - Google Patents
Method for preparing cyclocarya paliurus total flavonoid with effect of reducing blood glucose Download PDFInfo
- Publication number
- CN104013669A CN104013669A CN201410293019.XA CN201410293019A CN104013669A CN 104013669 A CN104013669 A CN 104013669A CN 201410293019 A CN201410293019 A CN 201410293019A CN 104013669 A CN104013669 A CN 104013669A
- Authority
- CN
- China
- Prior art keywords
- ethanol
- cyclocarya paliurus
- total flavones
- paliurus iljinskaja
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a method for preparing total flavonoid from cyclocarya paliurus leaves with an effect reducing blood glucose. The method comprises the following steps: adding an 70-85% ethanol aqueous solution with the amount 8-12 times that of cyclocarya paliurus leaves into dried cyclocarya paliurus leaves; performing reflux extraction for three times and 2 hours each time, filtering, combining extracts, and concentrating; dissolving concentrated dry extract with 75% ethanol, stirring with polyamide of 30-60 meshes in a ratio of 1: (1-3); passing through a column in a ratio of 1: (10-15); respectively eluting with water, 40% ethanol, 45-60% ethanol and 80-90% ethanol, wherein 10 column volumes are eluted in each gradient; the 45-60% ethanol part is divided into two sections, which refers to 45-60% ethanol upper part (first five column volumes) and 45-60% ethanol lower part (last five column volumes); and the 45-60% ethanol upper part and the 45-60% ethanol lower part are combined to form total flavonoid with content of over 50 percent. Pharmacological experiments indicate that the cyclocarya paliurus total flavonoid has an excellent blood glucose reducing effect.
Description
Technical field
The present invention relates to from leaf of Cyclocarya paliurus Iljinskaja, obtain having the total flavones preparation method of hypoglycemic activity.
Background technology
Diabetes are a kind of to cause sugar, albumen, fat, the metabolism disorder of power and water solution matter because insulin secretion is enough to and target tissue cell reduces insulin sensitivity relatively or absolutely not, are common Multiple Chronic diseases.With cancer, cardiovascular disease and be called the large disease in the world three, maximum feature is long and complicated, the difficult radical cure of the course of disease.Clinical insulin-dependent (1 type) and non-insulin-depending type (2 type) two classes of being divided into, wherein type 2 diabetes mellitus accounts for 90%～95%.Type 2 diabetes mellitus, must strictly control blood sugar level and relevant risk factor and could improve patient's life quality.At present approximately there are 3.6 hundred million diabeticss in the whole world, and in 1985, whole world diabetics only had 3,000 ten thousand, 2000 years to be increased to 1.5 hundred million, expects the year two thousand thirty will head direct for 600,000,000.Along with the change of economic fast development, life style and the prolongation of population life, diabetes mellitus in China prevalence rapid development.At present 20 years old above maturity-onset diabetes prevalence of China exceeded 10%, separately has 16% in diabetes edge, and the diabetics of estimating every day and newly increasing is about 3000 examples.China is existing diabetics number approximately 9,400 ten thousand at present, has replaced India, becomes the maximum country of diabetes number in the world.Hyperglycemia is the outstanding feature of diabetes, and hyperglycemia can cause microangiopathies, makes the microcirculation of diabetics have in various degree abnormal.Along with prolongation and the treatment of the course of disease can impel increasing the weight of and developing of microangiopathies not in time, cause the various acute and chronic complication of whole body, involve multiple Organ and tissues, cause blind, renal failure, cardiovascular disease, neuropathy, extremity gangrene etc., even dead.Diabetes have become the third-largest " Health Killer " that after cardiovascular diseases, malignant tumor, cause mankind's death, and the whole world has 4,000,000 people to die from diabetes or diabetic complication every year at present.Must strictly control the blood sugar level of diabetes and relevant risk factor and could improve patient's life quality.
Cyclocarya paliurus Iljinskaja (Cyclocarya paliurus (Batal) Ijinskaja) claims that again money tree, numb willow are Dicotyledoneae Juglandaceae cyclocarya plant; it is the endangered plants kind of the distinctive national second class protection seeds of China and special-protection-by-the-State; be distributed widely in 13 provinces and regions such as Jiangxi, Guangxi, Sichuan, Guizhou, Hubei (Chinese Plants will editorial board of the Chinese Academy of Sciences. Chinese Plants will [M]. the 21st volume. Beijing: Science Press, 1979:118-1911).Cyclocarya paliurus Iljinskaja has abundant bioactive ingredients and higher medical value, its leaves, bark, tree root can be used as medicine, warm in nature, the micro-hardship of acrid in the mouth, have clearing heat for detumescence, removing toxic substances, pain-relieving functions (Chinese Medicinal Materials Co. Chinese medicine resource will is wanted [M]. Beijing: Science Press, 1994); Modern pharmacology shows, Cyclocarya paliurus Iljinskaja extract has the physiologically actives such as blood sugar lowering, blood pressure lowering, blood fat reducing, strengthen in addition body immunity, antioxidation, the physiological function such as anticancer simultaneously, it is preventing and treating the particularly outstanding (Wang Kequan of effect aspect diabetes, Cao Ying. the progress [J] of Cyclocarya paliurus Iljinskaja chemical composition and pharmacological action. Heilungkiang medical science .2007,8 (31): 577-579).Zoopery and clinical research are found, leaf of Cyclocarya paliurus Iljinskaja and extract thereof or effective site have obvious hypoglycemic activity, can effectively reduce mice post-prandial glycemia, improve glucose tolerance in mice, blood glucose in diabetic mice, cholesterol and content of triglyceride due to reduction high glucose and high fat, and can improve insulin resistant.
Summary of the invention
The object of this invention is to provide a kind of preparation method of the Cyclocarya paliurus Iljinskaja total flavones with hypoglycemic activity; Preparation method technological operation of the present invention is simple, and stable performance, Cyclocarya paliurus Iljinskaja total flavones that pharmacologically active is good can be provided.
The preparation method with the Cyclocarya paliurus Iljinskaja total flavones of hypoglycemic activity, comprises the following steps:
(1) extract: get the dried leaves of Cyclocarya paliurus Iljinskaja, with ethanol water immersion, extract, obtain extracting solution, the concentrated dry extract that to obtain
(2) polyamide column chromatography: the extractum that step (1) is obtained, add dissolve with ethanol, mix upper polyamide column after sample with polyamide, carry out eluting with the alcoholic solution of different gradients respectively again, obtain eluent, concentrated, volatilize solvent and obtain Cyclocarya paliurus Iljinskaja total flavones.
Above-mentioned steps (1) ethanol water concentration used is 70～85%, adds the extraction solvent of 8～12 times of weight that are equivalent to medical material weight at every turn, heating and refluxing extraction, and each extraction keeps slight boiling condition 2h, extracts 3 times.
In above-mentioned steps (2), polyamide is 30～60 orders, and mixing sample concentration of alcohol used is 75%, and extractum and polyamide are mixed sample by 1: 1～3, by 1: 10～15 upper props.
The alcoholic solution of the different gradients described in above-mentioned steps (2) is respectively water, 40% ethanol, 45～60% ethanol, 80～90% ethanol; And 10 column volumes of the equal eluting of each gradient, wherein 45～60% ethanol positions are divided two sections, on 45～60% ethanol under (front 5 column volumes) and 45～60% ethanol (rear 5 column volumes); Under 45～60% ethanol, part and the merging of 80～90% ethanol parts are total flavones, and content reaches more than 50%.
Cyclocarya paliurus Iljinskaja general flavone content in the present invention has reached more than 50%, can be made into any acceptable preparation on pharmaceutics, for example, make oral Preparation: tablet, pill, capsule, granule, drop pill, oral liquid etc.
Cyclocarya paliurus Iljinskaja total flavones prepared by preparation method of the present invention, taking acarbose as contrast, variable concentrations group is set and has carried out external alpha-glucosidase activity inhibition experiment, result shows that Cyclocarya paliurus Iljinskaja total flavones has very strong inhibitory action to alpha-glucosidase; The IC50 value of acarbose and Cyclocarya paliurus Iljinskaja total flavones is respectively 58.608mg/L and 45.691mg/L.
Cyclocarya paliurus Iljinskaja total flavones prepared by preparation method of the present invention is in experiment (carbohydrate tolerance experiment) research that reduces mouse blood sugar, 40 mices are divided into 5 groups, every group 8, be respectively blank group, sucrose group, sucrose+total flavones group, starch group, starch+total flavones group, taking each group of mouse blood sugar concentration as index, get blood mode with eye socket and get 1h and 2h mice plasma after gavage and measure blood sugar concentration, result shows that Cyclocarya paliurus Iljinskaja total flavones has good hypoglycemic activity.
In the present invention, the preparation process of Cyclocarya paliurus Iljinskaja total flavones is few, easy and simple to handle, and cost is low, and general flavone content is high; And Cyclocarya paliurus Iljinskaja total flavones has very strong inhibitory action to alpha-glucosidase, effectively blood sugar lowering, can be used for the medicine of preparation prevention and treatment diabetes, has indicated direction for what further develop Cyclocarya paliurus Iljinskaja by value.
Brief description of the drawings
Starch, sucrose carbohydrate tolerance experimental result.
Detailed description of the invention
By specific embodiment, content of the present invention is described in further detail below, but these embodiment should never be understood to limitation of the present invention.Without departing from the idea case in the present invention described above, any variation that the invention process is done, within being all included in the present invention for those skilled in the art.
The preparation of embodiment 1 Cyclocarya paliurus Iljinskaja total flavones
(1) extract: take Cyclocarya paliurus Iljinskaja dry raw leaf 0.8Kg, with 75% alcohol reflux 3 times, each 2h, at every turn ethanol consumption 8000ml.Merge extractive liquid, also reclaims ethanol and obtains ethanol extraction dry extract.
(2) polyamide column chromatography: take processed good polyamide 125g (30-60 order), wet method dress post (post 5cm × 70cm), column volume (BV) 750ml.Take again ethanol extract dry extract 10.0g, use 75% dissolve with ethanol, admix in processed good polyamide 20g, volatilize ethanol, pack in post.Respectively (rear 5 column volumes), 80% ethanol under (front 5 column volumes), 50%7 alcohol on water, 40% ethanol, 50% ethanol, each gradient elution 10BV.Will be under 50% ethanol and 80% ethanol part merge, with aluminum nitrate determination of color general flavone content be 55.66%.
The inhibitory action of embodiment 2 Cyclocarya paliurus Iljinskaja total flavones to alpha-glucosidase activity
Cyclocarya paliurus Iljinskaja total flavones is divided into blank group, acarbose variable concentrations group (final concentration is respectively 250mg/L, 125mg/L, 62.50mg/L, 31.25mg/L, 15.63mg/L, 7.81mg/L, 7.81mg/L, 0mg/L) and total flavones variable concentrations group (final concentration is respectively 250mg/L, 125mg/L, 62.50mg/L, 31.25mg/L, 15.63mg/L, 7.81mg/L, 7.81mg/L, 0mg/L) to the inhibitory action experiment of alpha-glucosidase activity.Operating process formula is in test tube, to add respectively phosphate buffer 2mL, NPG (4-nitrophenol-α-D pyranglucoside) solution 0.2mL, acarbose or Cyclocarya paliurus Iljinskaja total flavones solution 0.1mL, blank group adds PBS0.3mL, in 37 DEG C of water bath with thermostatic control 10min; Add again alpha-glucosaccharase enzymatic solution 0.2mL (blank group is not added), in 37 DEG C of water bath with thermostatic control 10min, add ethanol 1.5mL cessation reaction, use Shimadzu U V-120-02 ultraviolet-uisible spectrophotometer to measure light absorption value at 405nm place.Inhibition of enzyme activity rate formula is as follows:
(A
negative control: absorbance when each position content is 0mg/L)
The inhibitory action experimental result of subordinate list 1 Cyclocarya paliurus Iljinskaja total flavones to alpha-glucosidase activity
The IC of acarbose and Cyclocarya paliurus Iljinskaja total flavones
50value is respectively 58.608mg/L and 45.691mg/L.Experimental data is processed with SPSS17.0 software.
Embodiment 3 Cyclocarya paliurus Iljinskaja total flavones carbohydrate tolerance experiments
Laboratory animal: ICR male mice, purchased from Nanjing Qinglongshan Experimental Animal Center, weight range: 18～22g.
Experimental technique
ICR male mice adaptability is raised 2-3d, is divided at random blank group, sucrose group, sucrose+total flavones group, starch group, starch+total flavones group, and 8 every group, before experiment, mice is 20h on an empty stomach.Blank group: gavage 1ml normal saline; Sucrose group: gavage 1ml sucrose solution (pressing 3g/Kg dosage with normal saline prepares); Sucrose+total flavones group: simultaneously gavage 0.5ml sucrose solution (press the preparation of 3g/Kg dosage with normal saline) and 0.5ml total flavones (preparing by 600mg/Kg dosage with normal saline); Starch group: gavage 1ml starch solution (pressing 3g/Kg dosage with normal saline prepares); Starch+total flavones group: simultaneously gavage 0.5ml starch solution (press the preparation of 3g/Kg dosage with normal saline) and 0.5ml80% ethanol position (preparing by 600mg/Kg dosage with normal saline).After each treated animal gavage 0,1,2h eye socket gets blood, measures the blood glucose value of each time point with glucose oxidase method test kit.Experimental result sees attached list 2.
Subordinate list 2 total flavones carbohydrate tolerance experimental results
Experimental data to be to carry out statistical analysis with SPSS17.0 software, and result represents with Mean ± SD, and " p# < 0.001 " represents to have significant difference between blank group and model group; " p** < 0.01, p*** < 0.001 ", represents to have significant difference between model group and administration group.
Total flavones and sucrose, starch mix to mouse stomach, blood glucose value after 1h, 2h is all lower than the blood glucose value of 1h, 2h after sucrose, the independent gavage of starch, difference has significance (p < 0.01) meaning, illustrates that total flavones has good effect to reducing mouse blood sugar.
Claims (8)
1. the preparation method with the Cyclocarya paliurus Iljinskaja total flavones of hypoglycemic activity, comprises the following steps:
(1) extract: get the dried leaves of Cyclocarya paliurus Iljinskaja, with ethanol water immersion, extract, obtain extracting solution, the concentrated dry extract that to obtain;
(2) polyamide column chromatography: the extractum that step (1) is obtained, add dissolve with ethanol, mix upper polyamide column after sample with polyamide, carry out eluting with the alcoholic solution of different gradients respectively again, obtain eluent, concentrated, volatilize solvent and obtain Cyclocarya paliurus Iljinskaja total flavones.
2. the preparation method of Cyclocarya paliurus Iljinskaja total flavones according to claim 1, is characterized in that, in step (1), ethanol water concentration used is 70～85%.
3. the preparation method of Cyclocarya paliurus Iljinskaja total flavones according to claim 1, is characterized in that, in step (1), add the extraction solvent of 8～12 times of weight that are equivalent to medical material weight at every turn, heating and refluxing extraction, each extraction keeps slight boiling condition 2h, extracts 3 times.
4. the preparation method of Cyclocarya paliurus Iljinskaja total flavones according to claim 1, is characterized in that, in step (2), polyamide is 30～60 orders, and mixing sample concentration of alcohol used is 75%, and extractum and polyamide are mixed sample by 1: 1～3, by 1: 10～15 upper props.
5. the preparation method of Cyclocarya paliurus Iljinskaja total flavones according to claim 1, is characterized in that, in step (2), the alcoholic solution of described different gradients is respectively water, 40% ethanol, 45～60% ethanol, 80～90% ethanol.
6. the preparation method of Cyclocarya paliurus Iljinskaja total flavones according to claim 1, it is characterized in that, 10 column volumes of the equal eluting of alcoholic solution of different gradients described in step (2), wherein 45～60% ethanol positions are divided two sections, on 45～60% ethanol under (front 5 column volumes) and 45～60% ethanol (rear 5 column volumes).
7. the preparation method of Cyclocarya paliurus Iljinskaja total flavones according to claim 1, is characterized in that, in step (2), under 45～60% ethanol, part and 80～90% ethanol parts merging are total flavones, and content reaches more than 50%.
8. prepare Cyclocarya paliurus Iljinskaja total flavones in the application of preparing in hypoglycemic drug or health product according to the preparation method described in claim 1～7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410293019.XA CN104013669A (en) | 2014-06-24 | 2014-06-24 | Method for preparing cyclocarya paliurus total flavonoid with effect of reducing blood glucose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410293019.XA CN104013669A (en) | 2014-06-24 | 2014-06-24 | Method for preparing cyclocarya paliurus total flavonoid with effect of reducing blood glucose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104013669A true CN104013669A (en) | 2014-09-03 |
Family
ID=51430852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410293019.XA Pending CN104013669A (en) | 2014-06-24 | 2014-06-24 | Method for preparing cyclocarya paliurus total flavonoid with effect of reducing blood glucose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104013669A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104305173A (en) * | 2014-09-25 | 2015-01-28 | 南京中医药大学 | Health food for diabetics and preparation method thereof |
| CN104940282A (en) * | 2015-06-05 | 2015-09-30 | 百花医花集团股份有限公司 | Cyclocarya paliurus preparation and preparation method thereof |
| CN112106910A (en) * | 2020-08-05 | 2020-12-22 | 宁波大学 | Soda water health beverage containing cyclocarya paliurus flavone and preparation method thereof |
-
2014
- 2014-06-24 CN CN201410293019.XA patent/CN104013669A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 杨武英等: "青钱柳黄酮对α-葡萄糖苷酶活性及小鼠血糖的影响", 《营养学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104305173A (en) * | 2014-09-25 | 2015-01-28 | 南京中医药大学 | Health food for diabetics and preparation method thereof |
| CN104940282A (en) * | 2015-06-05 | 2015-09-30 | 百花医花集团股份有限公司 | Cyclocarya paliurus preparation and preparation method thereof |
| CN112106910A (en) * | 2020-08-05 | 2020-12-22 | 宁波大学 | Soda water health beverage containing cyclocarya paliurus flavone and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115364170B (en) | Sugar metabolism regulator and preparation method and application thereof | |
| CN103070880A (en) | Application of acanthopanax trifoliatus polysaccharide in preparing medicine for treating diabetes | |
| CN101480422A (en) | Tibetan oriental wormwood extract as well as preparation method and use thereof | |
| CN101401829A (en) | Wild Jinchai liveness extract, preparation and uses thereof | |
| CN104013669A (en) | Method for preparing cyclocarya paliurus total flavonoid with effect of reducing blood glucose | |
| CN101095720B (en) | Method for preparing total salvianolic acid and the application in the preventing and treating of diabetes and the complication thereof | |
| CN102631526B (en) | Chinese medicinal composition for treating diabetes mellitus | |
| CN105125941A (en) | Preparation method of assistant hypoglycemic corn-stigma extractive capsules | |
| CN101933965A (en) | Mugwort extract and preparation process thereof, and application in controlling postmeal gucose | |
| CN102579530A (en) | Preparation method of aralia taibaiensis total saponin having diabetes mellitus resisting effect and medicament | |
| CN101849950A (en) | Application of rotundic acid in preparing blood lipid regulating medicines | |
| CN103520376A (en) | Chinese medicine health-maintenance preparation with auxiliary hypoglycemic activity | |
| CN103655791A (en) | Lotus leaf-based preparation for treating phlegm and blood stasis simultaneously and application thereof | |
| CN103432420B (en) | A kind of Chinese medicine composition for the treatment of diabetes and preparation method thereof and detection method | |
| CN102697910B (en) | Chinese medicinal extract dripping pill for treating diabetes and hyperlipidemia | |
| CN104523933A (en) | Traditional Chinese medicine for treating damp-heat jaundice, and preparation method, detection method and application thereof | |
| CN102363018A (en) | Throat stagnation eliminating Chinese medicinal composition and preparation method thereof | |
| CN102580042A (en) | Traditional Chinese medicine composition for treating wind-cold type of common cold and preparation method of traditional Chinese medicine composition | |
| CN102204990B (en) | Medicine extract for reducing sugar content and preventing diabetic angiopathy and preparation method thereof | |
| CN104523795A (en) | Application of cornua cervi pantotrichum glycopeptide composition in preparation of anti-anoxia drugs and health foods | |
| CN104277955A (en) | Traditional Chinese medicine wine for tonifying qi, nourishing blood and preserving health and preparation method thereof | |
| CN108853184A (en) | A kind of blue or green money willow and Pasania cuspidata extract combination | |
| CN102698096B (en) | Composition of traditional Chinese medicine extracts and preparation method and application thereof | |
| CN107684601B (en) | Compound granule capable of reducing blood sugar and preparation method thereof | |
| CN102526387A (en) | Medicinal composition for treating early-stage diabetic foot and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140903 |
|
| WD01 | Invention patent application deemed withdrawn after publication |