CN1026952C - Process for extracting effective component from basidiomycetes mycelium - Google Patents
Process for extracting effective component from basidiomycetes mycelium Download PDFInfo
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- CN1026952C CN1026952C CN 85106023 CN85106023A CN1026952C CN 1026952 C CN1026952 C CN 1026952C CN 85106023 CN85106023 CN 85106023 CN 85106023 A CN85106023 A CN 85106023A CN 1026952 C CN1026952 C CN 1026952C
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Abstract
The present invention provides a method for extracting an effective component from basidiomycetes mycelium, which is characterized in that basidiomycetes is inoculated to a solid culture medium, a culture medium containing mycelium is loosened, water, lower alcohol, and extraction solution prepared form one or more than one kind of enzyme are added to the solid culture medium, then the solid culture medium is pulverized and ground, and an enzyme lose activity through heating and is sterilized finally. A product obtained by the method can be used for treating high blood pressure, tinea pedis and acne, and a therapeutic effect is very ideal.
Description
The present invention relates to the method for effective component extracting from Basidiomycetes mycelium, more particularly just relate to a kind of method of extracting various effective ingredient from contain mycelial solid medium effectively, described mycelium is to obtain after making load culture propagations such as Lentinus Edodes on the solid medium that is matrix material with the bagasse.
The existing extensive studies of drug effect problem about the Basidiomycetes mycelium of Lentinus Edodes etc. in addition, about extract the method for the effective ingredient of active ingredient or nutritious value from champignon mycelium, had proposed various imaginations and scheme already.For example, at the public clear 51-19 of spy, such record is just arranged: inoculating Lentinus Edodes in No. 013 communique by Testa oryzae is added in the solid medium that forms in the sawdust, make the mycelium breeding according to usual way, to produce then sporophore face preceding or afterwards soon culture medium pulverize and add entry, it is close to be placed in the container envelope after pH value is adjusted to 5.0, be warmed to 30～55 ℃, thereby promoted the metabolism of mycelium enzyme and metabolite, further carried out just suspension being filtered after the enzyme reaction feature of the method for extraction active ingredient from the solid culture mycelium of Lentinus Edodes described in Here it is above-mentioned this patent gazette fully.In addition, special public according to 53-23, following record is also arranged in No. 392 communiques: with Semen arachidis hypogaeae epidermis or bagasse is matrix material, thereby add Testa oryzae more as required and form solid medium, at this inoculation of medium Lentinus Edodes, making the mycelium breeding contain mycelial culture medium to this is more afterwards pulverized, regulate pH value then and add entry, be placed in the container again and seal, be warmed to 30 ℃～55 ℃, the reaction of enzyme is carried out fully promoting that mycelium is metabolic, and then the suspension that is obtained filtered, Here it is, and this patent gazette is described, produce the feature of method of the health beverage agent of the effective ingredient that contains champignon mycelium.
Yet, at the public clear 51-19 of spy, exist following shortcoming in No. 013 described method of communique: since in the sawdust contain lignin and tannin enters into the extracting solution that is obtained, very bitter, so be not suitable as beverage.In addition, at the public clear 53-23 of spy, also there is following shortcoming in No. 392 described methods of communique: from the solid medium that contains champignon mycelium during effective component extracting, must regulate pH value, but also must remain on for a long time in 30 ℃～55 ℃ the temperature, this is complicated and time-consuming with regard to making process management.In addition, the bagasse that uses as solid medium because its cellulose is very hard, although it contains effective ingredient, but still can not be utilized, thereby has to discard.And sixty-four dollar question is to be impossible extract effective ingredient from the solid medium that contains champignon mycelium fully with these methods.
The present invention produces for solving above-mentioned these problems, to contain the mycelial bagasses of load such as champignon mycelium is that matrix material constitutes solid medium, from this solid medium, effectively and in a short period of time obtain the composition of active ingredient or nutritious value, and do not need the pH value of extracting solution is regulated, and can also be the fertilizer of the sugarcane fiber of side-product as solid residue, feedstuff or foodstuff come effectively to be utilized, and provide a kind of as above-mentioned described, the method that extracts active ingredient from the basidiomycetes filament is a purpose of the present invention.
Of the present invention from Basidiomycetes mycelium the method for effective component extracting have following feature, on the solid medium that with the bagasse is matrix material, inoculate basidiomycetes, then, unclamp the solid medium that contains mycelium (obtaining) by mycelial breeding, make and wherein can account for below 30% the percentage by weight by the part of No. 12 sieve apertures, then in the solid medium that this unclamps water, lower alcohols and by the fiber toxenzyme, the extracting solution that select enzyme more than a kind or a kind is formed in protease or the glycosidase adds on the solid medium that remains on 30 ℃～55 ℃ of temperature, after this, exist under the situation of enzyme above-mentioned solid medium pulverizing and grinding, so that percentage by weight can be under No. 12 mesh screens at the sugarcane fiber more than 70%, at last, by the temperature that is heated to 95 ℃ enzyme is lost activity and sterilize.
As the matrix material of solid medium among the present invention, used bagasse or in bagasse, be added with the material of Testa oryzae.Bagasse is to press the formed dried slag of the dregs that get off by Caulis Sacchari sinensis, this bagasse contains saccharide and the protein that constitutes the mycelium nutrient source, it can still also can add the Testa oryzae of 10～30 unit of weights as solid medium directly as solid medium in the bagasse of 100 unit of weights.
Be that the basidiomycetes of inoculating in the solid medium of matrix material can adopt Lentinus Edodes, Rhizoma Atractylodis macrocephalae's ear polyp etc. with the bagasse, but Lentinus Edodes is best.After basidiomycetes is inoculated into solid medium, in the time limit of defined, be positioned in the culturing room that temperature and humidity and illumination all regulated, so just can impel basidiomycetous breeding.
After mycelium fully spreads in that basidiomycetes is fully bred, unclamp the cellulose of bagasse culture medium in culture medium, allow to account for below 30% of gross weight from the weight of No. 12 mesh screen lower parts.When unclamping above-mentioned sugarcane fiber, if will to allow to from the weight of No. 12 mesh screen lower parts be more than 30%, that just must the special pulverizer of employing, is worthless therefore.In other words, when under the situation of not using special pulverizer, unclamping the bagasse culture medium, can just can only be below 30% from the weight of No. 12 mesh screen lower parts.
In the bagasse culture medium of unclamping in this way and remain in 30 ℃～50 ℃ the solid medium and add: (ⅰ) water, (ⅱ) lower alcohols, (ⅲ) extracting solution of forming by select enzyme more than a kind or a kind in cellulase, protease or the glycosidase.As the enzyme that is added, reasonable have cellulase or protease, but cellulase is even more ideal.Concerning 1 kilogram bagasse culture medium, the addition of required enzyme is 0.5～5 gram, but preferably 1～3 restrains.Concerning water, preferably do not contain metal ion plasma class material in addition, so, concerning 1 kilogram bagasse culture medium, will add 1～10 kilogram, but preferably add 2～6 kilograms, again, the present invention does not need to regulate the pH value that adds water.
Can adopt methanol, ethanol etc. as the lower alcohols that is added, wherein, ethanol is ideal.The weight percent content of this lower alcohol in extracting solution to be being advisable more than 5%, but better be between 10%～50%, and it would be desirable between 15%～30%.Owing to make and have lower alcohol in the extracting solution, therefore compare with aqueous extracting solution only, just can more effectively from the solid medium that contains Basidiomycetes mycelium, extract effective ingredient.
Then again mixture so synthetic, that be made up of bagasse culture medium, water, lower alcohol and enzyme is pulverized and is ground, thus make the sugarcane fiber percentage by weight can be under No. 12 mesh screens more than at least 70%.Above-mentioned pulverizing and grinding under the temperature that operation can remain on 30 ℃～50 ℃ is carried out, and the effect of pulverizing and grinding and the rising of temperature are carried out simultaneously, while but temperature is slowly risen carry out these operations.The pulverizing of sugarcane fiber and grinding adopt the change-speed gearing pump to carry out, Yi Bian promptly make the above-mentioned mixture that contains sugarcane fiber produce circulation, Yi Bian sugarcane fiber is applied the effect of pulverizing and grinding at gear parts.In addition, on one side the mixture that contains solid medium also available pump is made it circulation, Yi Bian establish pulping machine in addition again so that come solid medium is ground by this pulping machine.Sugarcane fiber through pulverizing and grinding wherein has at least 70% of the percentage by weight must be under No. 12 mesh screens.From No. 12 undersized parts of sieve aperture if be less than 70% percentage by weight, so, not only can not from solid medium, extract effective ingredient fully, and can not get abundant remollescent part in the cellulose and also can increase, so, just can not come effectively to have been utilized as feedstuff, foodstuff or fertilizer to the solid residue that is obtained, therefore, this get.
Then, will preferably be heated to 75 ℃～90 ℃, and make the cellulase, protease or the glycosidase that have added, or originally lose activity in the bagasse, sterilize simultaneously with regard to the enzyme that contains through the mixture heated to 95 that contains bagasse handled like this ℃.By being heated, extracting solution can make enzyme promote activity.Thereby prevented the rotten of the effective ingredient that extracted.
In addition, in this heating process, can also remove lower alcohols such as containing ethanol in the extracting solution by evaporation.In case of necessity, also can under the condition of decompression, carry out above-mentioned heating process with the evaporation of accelerating lower alcohol with remove.
The mixture that contains bagasse of Huo Deing in this way, with the filter cloth of 50～No. 120 meshes, preferably the filter cloth of 60～No. 100 meshes filters, thereby can remove the solid constituent that swims in extracting solution.Again, the filtration operation that contains the bagasse mixture also can be carried out before final heat sterilization operation, because under this occasion, get back to again in the solution part and go thereby all will squeeze the filtrate that squeezing is come out after each the filtration residue, therefore also have another advantage that the rate of can trying to achieve improves.
In the extracting solution that obtains by said method, contain a large amount of various amino acidses, vitamins and polysaccharide etc., but also present slightly milky filbertly, also exist small floating material in the liquid sometimes.The floating material that these are small, except the destruction thing that comes from culture medium, some then is protein and the starchy material that solidifies because of enzyme reaction and heating.This small floating material itself just has nutritive value.When we feel that to the existence of these floating materials some is worried, also can make the floating material precipitation obtain afterwards to separate by the static placement of a period of time, perhaps reach isolating purpose by using the very thin filter cloth of mesh to wait.
The extracting solution that contains effective ingredient that is obtained according to the present invention has hypotensive activity, diuresis, antibacterial action, the effect that sets up, recovers the effect of pachylosis effect and treatment acne.In addition, therefore extracting solution involved in the present invention also can look to it to have stronger anti-tumor activity owing to contain the polysaccharide of lentinan etc.
The present invention by on the solid medium that unclamps, adding water, ethanol etc. lower alcohol and the enzyme of cellulase, protease etc. come the decomposition of accelerating solid culture medium and the enzyme reaction of mycelium self.So that can from solid medium, extract active ingredient or the nutritious effective ingredient that is worth in the material that becomes to grade in the short period of time expeditiously.In addition, owing to be in the solid medium that unclamps, to add under the state of the extracting solution of forming by water, lower alcohols and enzyme solid medium is applied pulverizing and grinding effect thereof, so can promptly extract effective ingredient.On the other hand, therefore the solid medium cellulose that has unclamped, can provide and make fertilizer, feedstuff or foodstuff usefulness because the effect of enzyme and pulverizing and grinding has become very trickle and soft.
In addition, in the extracting solution that contains the effective ingredient that from Basidiomycetes mycelium, obtains by above-mentioned approach, add dextrin, lactose, thereby make shapes such as tablet, pill and powder as setting agent.
Below, will come the present invention is done detailed explanation according to embodiment, but the present invention be subjected to placing restrictions on of these embodiment.
Embodiment 1
The solid medium of being made up of the Testa oryzae of the bagasse of 85 unit of weights and 15 unit of weights is heated to 120 ℃ in advance sterilizes, make it contain an amount of pure water then, inoculate Lentinus Edodes afterwards, make mycelium obtain breeding thereby be placed in all adjusted culturing room of having got well of temperature and humidity.After mycelium spreads to solid medium, remain on below 24% thereby again the cellulose of bagasse base material is unclamped the weight that allows to from No. 12 mesh screen lower parts.In 1.0 kilograms of this culture medium of unclamping, formed the mixture that contains bagasse on the solid medium that remains on 40 ℃ of temperature thereby the extracting solution of being made up of 2.5 milliliters of pure water, 0.8 milliliter of ethanol and refined cellulose enzyme 2.0 grams added to.
Then, adopt the change-speed gearing pump to make this mixture that contains culture medium circulation on one side again, on one side solid medium is applied pulverizing and grinding effect about 200 minutes at the gear parts of pump, can be thereby about 80% of sugarcane fiber percentage by weight is become from No. 12 undersized siftage of sieve aperture.The pulverizing and the grinding thereof that contain the mixture of bagasse are to carry out when the temperature that makes this mixture slowly rises.After this, also to further heat, and under 90 ℃ temperature, place 30 minutes this mixture that contains bagasse.Enzyme is lost activity and sterilize by the process that is heated to 90 ℃.Evaporation and remove ethanol contained in the extracting solution in during this period of time.Filter cloth with No. 60 meshes filters the mixture that contains culture medium that is obtained, so just obtained to contain the extracting solution of the effective ingredient that extracts from champignon mycelium.On the other hand, also obtained to pulverize to such an extent that very thin solid is grown slag, and the feedstuff as domestic animals such as cattle after these residue dryings is provided.
The scale of contained various effective ingredient is shown in table 1 in the extracting solution according to above-mentioned approach acquisition.
On the other hand, as a comparative example, the mixture that we do not have to contain bagasse is pulverized and is ground, nor add ethanol and cellulase, in addition all other are still taked the way identical with embodiment 1, from champignon mycelium, produce extracting solution, and the amount of various effective ingredient contained in this extracting solution is also illustrated in table 1.
Table 1
Embodiment 1 comparative example
Protein 4.0 grams 0.1 gram
Fat constituent 2.0 grams 0.1 gram
Carbohydrate undetermined 0.3 gram
Content of ashes 3.0 grams 0.1 gram
Vitamin B1 0.09 gram trace
0.01 milligram of vitamin B2 0.18 gram
0.4 milligram of glutamic acid 0.32 gram
0.2 milligram of leucine 0.20 gram
0.2 milligram of alanine 0.16 gram
0.2 milligram of glycine 0.15 gram
We can see from table 1, when from solid medium, extracting effective ingredient, by the mixture that contains bagasse being pulverized and ground and adding lower alcohol and cellulase, just can from solid medium, extract effective ingredient very effectively.
Embodiment 2
As the enzyme that should add, here do not adopt the refined cellulose enzyme of 2.0 grams, but adding the refined cellulose enzymes of 1.5 grams and the refining protease of 0.5 gram, in addition all other all adopt the way identical with embodiment 1, have extracted effective ingredient from champignon mycelium.
Embodiment 3
As the enzyme that should add, here do not adopt the refined cellulose enzymes of 20 grams, but added the refining protease of 3.0 grams, and solid medium remained under 42 ℃ the temperature, in addition all other all adopt the way identical with embodiment 1, have made extracting solution at last.
The extracting solution that is obtained is the same with embodiment 1, contains the very various effective ingredient of high concentration.
Embodiment 1 is obtained, the extracting solution that contains the effective ingredient that from champignon mycelium, extracts, allow the age be that 55 years old man is with every day three times, each 20 milliliters dosage is taken, its result, and the average maximal blood pressure before taking is 170, in 30 days after taking, average maximal blood pressure becomes 145, and in 60 days after taking, average maximal blood pressure becomes 143.
Embodiment 5
Here the antibacterial ability that has shown the extracting solution that embodiment 1 is obtained.This antibacterial ability is to carry out lyophilization by the extracting solution that embodiment 1 is obtained, and then is applied in the various mushrooms, just determines after the antibacterial ability of its antibacterial ability and traditional antibacterial methyl parahydroxybenzoate is made comparisons.Its result is as shown in table 2.
Table 2
Test organisms para hydroxybenzene Lentinus Edodes mycelia
Methyl formate body extracting solution
Trace (%)
C.albicans IFO-1549 0.2 ＞3
St.aureus 209P ＞0.2 1
M.lysodeikticus ATCC 4698 ＞0.2 0.25
Ps.aerugirosa ATCC 10145 ＞0.2 0.5
E.coli K-12 OUT 8041 ＞0.025 1
S.typhimurium 0.05 1
Kl.pneumoniae OUT 8017 0.2 1
Sec.marcescens 0.1 1
Asp.niger IFO 4407 0.05 ＞3
From table 2 we as can be seen, the extracting solution that contains the effective ingredient that extracts from champignon mycelium has very good antibacterial ability.
Embodiment 6
Use the extracting solution of the mushroom fungus filament that obtains among the embodiment 1, modulated the butterfat constituent that contains following each composition.
Paraffin 4.0 unit of weights
Micro-crystallization wax 6.0 unit of weights
Peak wax 6.0 unit of weights
Vaseline 14.0 unit of weights
Liquid Paraffin 42.5 unit of weights
NOFABLE SO-992 NOFABLE SO-902 3.7 unit of weights
Polychlorostyrene ethylene sorbitan 0.8 unit of weight
Monoleate (20E.0)
Champignon mycelium extracting solution 25.0 unit of weights
Chlorination bromo geramine 0.1 unit of weight
Spice, pigment are an amount of
This butterfat constituent all is coated on the skin that a man of 53 years old wrinkled because of the tinea pedis chap every day, and the thick wrinkle of skin surface partly just begins to have recovered after several days.
Embodiment 7
Employing has been modulated the butterfat constituent that contains following each composition from the extracting solution that gets the mushroom fungus filament that embodiment 1 is obtained.
Cera Flava 10 unit of weights
Liquid Paraffin 50 unit of weights
Champignon mycelium extracting solution 35 unit of weights
Chlorination bromo geramine 0.05 unit of weight
Pigment, spice are an amount of
This butterfat constituent, every day is early, middle and late to be coated in for three times around the acne that people grow on the face, is coated with for four weeks continuously.
Just begin after first week to see that acne dwindling gradually, after four weeks, acne on the face almost can't see, and it is quite bright and clean that the surface of skin becomes.
In addition, both made all coat a little this butterfat constituents every day, also can't see zest vestiges such as macule fully on the face.
Claims (1)
1, a kind of method of from the Lentinus Edodes mycelium, extracting active ingredient, it is characterized in that, on the solid medium that with the bagasse is matrix material, inoculate Lentinus Edodes, then, unclamp and contain the mycelial solid medium that obtains by mycelial breeding, make wherein and can account for below 30% of gross weight by the weight of No. 12 sieve aperture parts, in the solid medium that this unclamps, add then by water, ethanol and from cellulase, the extracting solution that select enzyme more than a kind or a kind is formed in protease or the glycosidase, ethanol is to deposit with the percentage by weight of 15%-30% in the extracting solution, at this moment, the temperature of solid medium should remain in 30 ℃～50 ℃, then, above-mentioned solid medium is pulverized and ground existing under the situation of above-mentioned enzyme, cause this fiber more than at least 70% that accounts for sugarcane fiber weight can pass through No. 12 sieve apertures, at last, make above-mentioned enzyme lose activity and sterilize, filtered then by the temperature that is heated to 95 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 85106023 CN1026952C (en) | 1985-07-30 | 1985-07-30 | Process for extracting effective component from basidiomycetes mycelium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 85106023 CN1026952C (en) | 1985-07-30 | 1985-07-30 | Process for extracting effective component from basidiomycetes mycelium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN85106023A CN85106023A (en) | 1987-02-04 |
| CN1026952C true CN1026952C (en) | 1994-12-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 85106023 Expired - Lifetime CN1026952C (en) | 1985-07-30 | 1985-07-30 | Process for extracting effective component from basidiomycetes mycelium |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4203965B2 (en) * | 2003-05-01 | 2009-01-07 | マイコロジーテクノ株式会社 | Bassidiomycetes, Bassidiomycetes extract composition, health food and immunostimulant |
| CN110074140B (en) * | 2019-05-28 | 2020-06-09 | 山东省花生研究所 | Biocontrol microbial inoculum for producing aspergillus flavus, preparation method and application thereof |
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1985
- 1985-07-30 CN CN 85106023 patent/CN1026952C/en not_active Expired - Lifetime
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| Publication number | Publication date |
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| CN85106023A (en) | 1987-02-04 |
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