CN102687634A - Method for cultivating rosemary - Google Patents
Method for cultivating rosemary Download PDFInfo
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- CN102687634A CN102687634A CN2012101589974A CN201210158997A CN102687634A CN 102687634 A CN102687634 A CN 102687634A CN 2012101589974 A CN2012101589974 A CN 2012101589974A CN 201210158997 A CN201210158997 A CN 201210158997A CN 102687634 A CN102687634 A CN 102687634A
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Abstract
The invnetion discloses a method for cultivating rosemary. The method provided by the invention comprises the following steps of: 1), irrigating the rosemary from the end of October to the beginning of November in the north area, at the same time, erecting a plastic shelter, and covering coverings on the outer surface of the plastic shelter; 2), removing the coverings treated in the step 1) at the end of February in the next year; and 3), removing the plastic shelter treated in the step 2) at the end of March in the next year and irrigating the rosemary. Experimental results show that the method disclosed by the invention performs cold-proof cultivation in Beijing, and avoids the cultivation difficulty in the north area; the bacteriostatic activity of an essential oil extracted from the cultivated rosemary is high, so that the essential oil has inhibition effects on escherichia coli, staphylococcus aureus, bacillus subtilis and proteus.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of cultivate rosemary, method.
Background technology
Rosemary, (Rosmarinusofficinalis L.) is a Labiatae perennial evergreen undershrub, originates in Mediterranean Region, mainly is distributed in ground such as France, Spain, Turkey and Italy.Introduced a fine variety to China by Institute of Botany, Chinese Academy of Sciences's Beijing Botanical Garden in 1981; Extensively introduce a fine variety in each province, the whole nation as raw material and ornamental plants in garden now; Ground benign climates such as Guizhou, Yunnan, Guangxi, Hainan wherein, area under cultivation is bigger, first tool production scale.
The main component of Rosemary Oil is monoterpene and derivative thereof, comprises australene, 1, and 8-Cineole, camphor, (Pintore et al., 2002) such as amphene and borneol.According to iso standard, the chemical type of Rosemary Oil has two kinds, i.e. Tunisia-Morocco's type (Tunisian and Moroccan type) and Spain's type (Spanish type).The former is with 1, and the 8-Cineole is a main component, accounts for 38%-55%; Latter's australene, 1, the content of 8-Cineole, three kinds of compositions of camphor is similar, between 12.5%-26% (Bruneton, 1999).In addition also there are some uncommon odor types; For example Turkey's Rosemary Oil p-cymene/linalool/ γ-terpinen odor type, Portuguese myrcene/ α-pinene/1; The camphor/1 of 8-cineole odor type and Cuba, 8-cineole/borneol odor type (Ozcan and Chalchat, 2008; Serrano et al., 2002).According to bibliographical information, the odor type of real estate Rosemary Oils such as China Guizhou, Fujian, Yunnan, Xinjiang, Guangxi is Spain's type.
The Rosemary Oil chemical composition is various, receives influence of various factors, comprises external factors such as internal factor such as kind, strain age, vegetative stage and cultivation condition (temperature, light, water, fertilizer), plantation area, method for distilling, the position of drawing materials.
At present also do not have system research the survive the winter cultivation method of winter protection of northern area, therefore, seek the picking methods that protection ground cultivation method and cauline leaf separate about rosemary,, for efficient, sustainable use rosemary, resource is significant.
Summary of the invention
An object of the present invention is to provide a kind of cultivate rosemary, method.
Method provided by the invention comprises the steps:
1) at north latitude 39 " 26 ' to 41 " 03 ', east longitude 115 " 25 ' to 117 " 30 ' locate, at the beginning of 11 months, irrigate rosemary, by the end of October, set up plastic greenhouse simultaneously, and cover covering at the outer surface of said plastic greenhouse;
2) at the end of two months next years, remove the said covering of handling through step 1);
3) at the end of three months next years, removal will be through step 2) said plastic greenhouse after handling, irrigate simultaneously.
In the said method, in the step 1), said was 25-November 10 October at the beginning of 11 months by the end of October, 5 ℃ of mean ground temperatures in 5 centimetres of topsoil soils during this, and nighttime temperature is lower than 0 degree centigrade (5 ℃-0 ℃), and table soil freezes day to disappear night, and moisture can ooze down in soil;
Step 2) in, at the end of said two months 20-February 28 February,
In the step 3), it at the end of said three months 20-March 31 March.
In the said method, in the step 1), said north latitude 39 " 26 ' to 41 " 03 ', east longitude 115 " 25 ' to 117 " 30 ' locate to be the BeiJing, China;
Said irrigation volume is 40-50m
3Water/mu;
In the step 3), said irrigation volume is 30-35m
3Water/mu.
In the said method, in step 1) and step 2) between, comprise the steps: that also through the temperature in the said plastic greenhouse after the step 1) processing be 25 ℃-30 ℃ by day, said covering is opened ventilation, and continue to cover said covering at night;
Said covering is a straw screen or mat.
The rosemary, that is obtained by said method also is the scope that the present invention protects the application that preparation has in the rosemary, water extract of bacteriostatic activity; In the above-mentioned application, said bacterium further is specially Escherichia coli (Escherichia coli), Staphylococcus aureus (Staphylococcus aureus subsp.aureus), bacillus subtilis (Bacillus subtilis) and/or proteus (Proteus vulgaris).
Another object of the present invention provides a kind of method for preparing the rosemary, water extract.
Method provided by the invention comprises the steps:
The rosemary, of gathering earlier and obtaining by said method, again with said rosemary, tissue be immersed in the water, volatile oil is collected in distillation, promptly obtains the rosemary, water extract.
In the said method, above-mentioned rosemary, the proportioning of tissue and said water be 1g:10ml;
Said distillation is 180 ℃ of heated at constant temperature to obtaining first volatile oil, behind 2 h that seethe with excitement, finishes distillation.
In the said method, before said distillation, also comprise above-mentioned rosemary, dry, the step of pulverizing;
Before said distillation back and said collection volatile oil, comprise that also the product with said distillation leaves standstill the step of 15-20min;
Behind said collection volatile oil, also comprise said volatile oil dehydration, obtain the step of rosemary, water extract;
Said rosemary, be organized as stem or leaf.
Rosemary, water extract by method for preparing also is the scope that the present invention protects; Said rosemary, water extract specifically has bacteriostatic activity; Or described rosemary, water extract has in the bacteriostatic activity product in preparation and uses; Said bacterium further is specially Escherichia coli (Escherichia coli), Staphylococcus aureus (Staphylococcus aureus subsp.aureus), bacillus subtilis (Bacillus subtilis) and/or proteus (Proteus vulgaris).
Experiment of the present invention proves; The inventive method is for carrying out the winter protection cultivation in Beijing; Rosemary, is survived the winter in Beijing and normal growth, and also carry out the winter protection cultivation northern area at present to rosemary,, if do not carry out winter protection cultivate then rosemary, can be in Beijing winter dead; Can't survive the winter, this method has been avoided in northern area plantation difficulty.And rosemary, water extract (essential oil) bacteriostatic activity that cultivation obtains is higher, and Escherichia coli, staphylococcus aureus, bacillus subtilis and proteus are all had the inhibition effect.
Embodiment
Employed experimental technique is conventional method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Experiment among the following embodiment is triplicate, results averaged.
Embodiment 1, rosemary, winter protection cultivation method
1,2010 in the Beijing area (north latitude 39 " 26 ' to 41 " 03 ', east longitude 115 " 25 ' to 117 " 30 '), November 1, (mean ground temperature was 5 ℃ in 5 centimetres of topsoil soils; Nighttime temperature is lower than 0 degree centigrade; Be specially-5 ℃, table soil freezes day to disappear night, and moisture can ooze down in soil; Time is before the Beginning of Winter, promptly at the beginning of by the end of October to 11 months; ) at the disposable filling foot of rosemary, field gene bank water (50m
3Water/mu), set up plastic greenhouse immediately, in outer surface bedding straw screen or mat (Ji County, Tianjin straw-made articles store company of plastic greenhouse; CL1001); Guarantee artificial nurse simultaneously, go up (temperature of shed reaches 25 degrees centigrade) like daytime temperature winter or in time take off the curtain ventilation when sunny, put curtain night;
2, (be specially on February 25th, 2011) at the end of two months next years and remove straw screen or mat, manual work is simultaneously ventilated according to weather conditions;
3, (be specially on March 25th, 2011) at the end of three months next years and remove plastic greenhouse, and irrigate in time that (irrigation volume is 30m
3/ mu water).
The result survives the winter in Beijing and normal growth for making rosemary,, and also carries out the winter protection cultivation to rosemary, northern area at present, if do not carry out winter protection cultivate then rosemary, can be in Beijing winter dead, can't survive the winter.
The preparation of embodiment 2, rosemary, water extract and bacteriostatic activity detect
One, the preparation of rosemary, water extract
The rosemary, cauline leaf of gathering respectively on June 25th, 2011, drying in the shade obtains scapus and cured leaf, adopts steam distillation to extract essential oil respectively, specific as follows: adopt Clevenger type extraction element, extract Rosemary Oil through steam distillation, specific as follows:
With the rosemary, of plucking stem, leaf dry in the shade, pulverize; Take by weighing 100g rosemary, cured leaf and 100g rosemary, scapus respectively, place the round-bottomed flask of 2000ml, add 1000ml distilled water submerged plant material respectively; 180 ℃ of heated at constant temperature; After first essential oil oozes, pick up counting, behind the boiling 2h, finish distillation.After stopping heating, leave standstill 20min, treat water-oil separating after, get oil water separator at the middle and upper levels liquid be thick oil, use anhydrous sodium sulfate dehydration, must rosemary, stem water extract (essential oil) and rosemary leave water extract (essential oil).
Two, the bacteriostatic activity of rosemary, water extract detects
Bacteriostatic activity assay method: disk diffusion method; Bacterial classification to be measured (Escherichia coli Escherichia coli ATCC25922 (CGMCC 1.2385), Staphylococcus aureus Staphylococcus aureus subsp.Aureus ATCC25923 (CGMCC 1.2386), bacillus subtilis Bacillus subtilis ATCC6633 (CGMCC1.2428), proteus Proteus vulgaris ATCC49132 (CGMCC 1.491)) is cultivated with the beef extract-peptone liquid nutrient medium, used before the use than turbid appearance each bacterium liquid is diluted to 1x10
8CFUmL
-1The beef extract-peptone agar medium of sterilization is poured in the culture dish of diameter 9cm, after the culture medium solidifying, draws each bacterium liquid 100 μ l with liquid-transfering gun and evenly coats on the flat board, and the diameter 6mm filter paper of getting behind the hot air sterilization with tweezers then is smooth in dull and stereotyped central authorities.Essential oil to be measured is crossed 0.45 μ m miillpore filter, and liquid-transfering gun is drawn 4 μ l and dripped on filter paper, and each handles three repetitions.Distilled water is set makes blank, (penicillin, streptomycin and cefotaxime 1mgmL-1) are made positive control to antibiotic.After treating that essential oil is fully absorbed, be inverted for 37 ℃ and cultivated 24 hours, measure antibacterial circle diameter.
Above-mentioned essential oil to be measured is respectively rosemary, stem water extract (essential oil) and rosemary leave water extract (essential oil).
The result is as shown in table 1:
Table 1 is the bacteriostatic activity of rosemary, water extract
Find out that from above-mentioned rosemary leave water extract (essential oil) is strong to the inhibition ability of Escherichia coli and proteus, rosemary, stem water extract (essential oil) is strong to the inhibition ability of staphylococcus aureus and bacillus subtilis.
Claims (10)
- One kind cultivate rosemary, method, comprise the steps:1) at north latitude 39 " 26 ' to 41 " 03 ', east longitude 115 " 25 ' to 117 " 30 ' locate, at the beginning of 11 months, irrigate rosemary, by the end of October, set up plastic greenhouse simultaneously, and cover covering at the outer surface of said plastic greenhouse;2) at the end of two months next years, remove the said covering of handling through step 1);3) at the end of three months next years, removal will be through step 2) said plastic greenhouse after handling, irrigate simultaneously.
- 2. method according to claim 1 is characterized in that:In the step 1), said was 25-November 10 October at the beginning of 11 months by the end of October,Step 2) in, at the end of said two months 20-February 28 February,In the step 3), it at the end of said three months 20-March 31 March.
- 3. method according to claim 1 and 2 is characterized in that:In the step 1), said north latitude 39 " 26 ' to 41 " 03 ', east longitude 115 " 25 ' to 117 " 30 ' locate to be the BeiJing, China;Said irrigation volume is 40-50m 3Water/mu;In the step 3), said irrigation volume is 30-35m 3Water/mu.
- 4. according to arbitrary described method among the claim 1-3, it is characterized in that:In step 1) and step 2) between, comprise the steps: that also through the temperature in the said plastic greenhouse after the step 1) processing be 25 ℃-30 ℃ by day, said covering is opened ventilation, and continue to cover said covering at night; Said covering is a straw screen or mat.
- 5. the rosemary, that is obtained by the said method of claim 1-4 has the application in the rosemary, water extract of bacteriostatic activity preparation.
- 6. application according to claim 5 is characterized in that: said bacterium is Escherichia coli (Escherichia coli), Staphylococcus aureus (Staphylococcus aureussubsp.aureus), bacillus subtilis (Bacillus subtilis) and/or proteus (Proteus vulgaris).
- 7. a method for preparing the rosemary, water extract comprises the steps:The rosemary, of gathering earlier and obtaining by the said method of claim 1-4, again with said rosemary, tissue be immersed in the water, volatile oil is collected in distillation, promptly obtains the rosemary, water extract.
- 8. method according to claim 7 is characterized in that:Said rosemary, the proportioning of tissue and said water be 1g:10ml;Said distillation is 180 ℃ of heated at constant temperature to obtaining first volatile oil, behind the boiling 2h, finishes distillation.
- 9. according to claim 7 or 8 described methods, it is characterized in that:Before said distillation, also comprise with said rosemary, the step of dry, pulverizing;Before said distillation back and said collection volatile oil, comprise that also the product with said distillation leaves standstill the step of 15-20min;Behind said collection volatile oil, also comprise said volatile oil dehydration, obtain the step of rosemary, water extract;Said rosemary, be organized as stem or leaf.
- 10. the rosemary, water extract for preparing by arbitrary said method among the claim 7-9; Said rosemary, water extract specifically has bacteriostatic activity; Or described rosemary, water extract has in the bacteriostatic activity product in preparation and uses; Said bacterium further is specially Escherichia coli (Escherichia coli), Staphylococcus aureus (Staphylococcus aureussubsp.aureus), bacillus subtilis (Bacillus subtilis) and/or proteus (Proteus vulgaris).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101589974A CN102687634A (en) | 2012-05-21 | 2012-05-21 | Method for cultivating rosemary |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101589974A CN102687634A (en) | 2012-05-21 | 2012-05-21 | Method for cultivating rosemary |
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| CN102687634A true CN102687634A (en) | 2012-09-26 |
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| CN2012101589974A Pending CN102687634A (en) | 2012-05-21 | 2012-05-21 | Method for cultivating rosemary |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106068971A (en) * | 2016-05-26 | 2016-11-09 | 温立刚 | Special efficacy Herba Rosmarini Officinalis cultural method |
| CN107502450A (en) * | 2017-08-28 | 2017-12-22 | 广西科技大学 | Wooden grape essential oil and its application |
Citations (2)
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| CN1416674A (en) * | 2002-11-25 | 2003-05-14 | 黄立海 | Method of cultivating rosemery herb in opium poppy cultivating land |
| CN1723754A (en) * | 2004-07-23 | 2006-01-25 | 黄立海 | Method for growing plant rosemary for extracting natural antioxidant and essential oil |
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2012
- 2012-05-21 CN CN2012101589974A patent/CN102687634A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1416674A (en) * | 2002-11-25 | 2003-05-14 | 黄立海 | Method of cultivating rosemery herb in opium poppy cultivating land |
| CN1723754A (en) * | 2004-07-23 | 2006-01-25 | 黄立海 | Method for growing plant rosemary for extracting natural antioxidant and essential oil |
Non-Patent Citations (6)
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| 刘昭明等: "迷迭香挥发油成分及抑菌活性研究", 《安徽农业科学》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106068971A (en) * | 2016-05-26 | 2016-11-09 | 温立刚 | Special efficacy Herba Rosmarini Officinalis cultural method |
| CN107502450A (en) * | 2017-08-28 | 2017-12-22 | 广西科技大学 | Wooden grape essential oil and its application |
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Application publication date: 20120926 |