CN102676292A - Separation process of toad maggot essential oil - Google Patents
Separation process of toad maggot essential oil Download PDFInfo
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- CN102676292A CN102676292A CN2012101786050A CN201210178605A CN102676292A CN 102676292 A CN102676292 A CN 102676292A CN 2012101786050 A CN2012101786050 A CN 2012101786050A CN 201210178605 A CN201210178605 A CN 201210178605A CN 102676292 A CN102676292 A CN 102676292A
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- essential oil
- toad
- maggot
- toad maggot
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Abstract
The invention relates to an extraction separation process of animal fat, in particular to a separation process of toad maggot essential oil. Fat ingredients contained in toad maggots have strong antibacterial and antiviral biological activity functions. According to the technical scheme, the separation process comprises carrying out supercritical carbon dioxide extraction; collecting extract; adding the extract into petroleum ether and mixing, then adding activated clay and evenly mixing, carrying out centrifugal separation, and collecting leaching liquid; and warming the leaching liquid under vacuum, removing the petroleum ether by evaporation, and collecting the toad maggot essential oil. On the basis of guaranteeing biological activity of the toad maggot essential oil, the toad maggot essential oil prepared by separation through the special process has the advantages of high purity, low cost and easiness in industrial production.
Description
Technical field
The present invention relates to a kind of extraction and separation process of animal oil and fat, especially the separating technology of toad maggot essential oil.
Background technology
The toad maggot is to be raw material with the toad, handles the maggot that generates through Chinese medicine, contains a large amount of lubricant components in the maggot, and a large amount of basic researchs show that the lubricant component that the toad maggot contains has stronger antibiotic, antibacterial, antiviral bioactive functions.
Application number 201110007300.9 discloses the toad maggot and has separated toad peptide antibiotic and antimicrobial preparation method thereof; With the toad maggot is raw material; Adopt water solvent extraction process or dilute alkaline soln extraction process or dilute alcohol solution extraction process; One or more technology purification process in the technologies such as coupled ion exchange resin absorption method, ultrafiltration and micro-filtration, gel chromatography, ultrasonic standing wave liquid phantom preparing chromatogram, vacuum lyophilization is prepared into the toad peptide antibiotic again.
ZL200410013742.4 discloses and has adopted Chinese medicine to cultivate the method for toad maggot, can mass industrialized production toad maggot.
Animal oil and fat extraction and separation method commonly used has technologies such as milling process, organic solvent lixiviation process, heating stripping method.
Summary of the invention
The lubricant component that contains in view of the toad maggot has stronger antibiotic, antibacterial, antiviral bioactive functions, and the present invention provides a kind of separating technology of toad maggot essential oil.
The object of the invention can be realized through following technical proposals:
(1) the dried toad maggot of 1000g is packed in the supercritical carbon dioxide extraction apparatus, is that 10～30Mpa, temperature are that 20～50 ℃, flow are to extract 2～6h under the condition of 10～100L/min at pressure;
(2) supercritical carbon dioxide extraction apparatus pressure is reduced to 7～8Mpa, and temperature is to resolve under 30～35 ℃ of conditions, collects extraction liquid;
(3) extraction liquid is added to the abundant down 10～60min of stirring of normal temperature condition in the sherwood oil, adds 500～2000g atlapulgite again to stir, and normal temperature condition held 6～24h separates under 5000～15000r/min condition with whizzer, collects vat liquor;
(4) vat liquor is to heat 30～80 ℃ under the condition of 0.5～3Mpa in vacuum tightness, and sherwood oil is sloughed in evaporation, collects toad maggot essential oil.
Present method is to guarantee on the bioactive basis of toad maggot essential oil; The toad maggot essential oil that adopts special process to separate preparation have purity height, cost low, be easy to suitability for industrialized production, extraction efficiency high, save the energy, do not have the advantage of environmental pollution, isolating toad maggot essential oil concentration reaches 93～96%.
Embodiment
Embodiment one: this embodiment separates toad maggot essential oil according to following step:
(1) the dried toad maggot of 1000g is packed in the supercritical carbon dioxide extraction apparatus, is that 10～30Mpa, temperature are that 20～50 ℃, flow are to extract 2～6h under the condition of 10～100L/min at pressure;
(2) supercritical carbon dioxide extraction apparatus pressure is reduced to 7～8Mpa, and temperature is to resolve under 30～35 ℃ of conditions, collects extraction liquid;
(3) extraction liquid is added to the abundant down 10～60min of stirring of normal temperature condition in the sherwood oil, adds 500～2000g atlapulgite again to stir, and normal temperature condition held 6～24h separates under 5000～15000r/min condition with whizzer, collects vat liquor;
(4) vat liquor is to heat 30～80 ℃ under the condition of 0.5～3mpa in vacuum tightness, and sherwood oil is sloughed in evaporation, collects toad maggot essential oil;
(5) essential oil packing.
Embodiment two: this embodiment separates toad maggot essential oil according to following step:
(1) the dried toad maggot of 1000g is packed in the supercritical carbon dioxide extraction apparatus, is that 20Mpa, temperature are that 40 ℃, flow are to extract 5h under the condition of 60L/min at pressure;
(2) supercritical carbon dioxide extraction apparatus pressure is reduced to 7Mpa, and temperature is to resolve under 30 ℃ of conditions, collects extraction liquid;
(3) extraction liquid is added to the abundant down 30min of stirring of normal temperature condition in the sherwood oil, adds the 1000g atlapulgite again to stir, and normal temperature condition held 12h separates under the 10000r/min condition with whizzer, collects vat liquor;
(4) vat liquor is to heat 40 ℃ under the condition of 2mpa in vacuum tightness, and sherwood oil is sloughed in evaporation, collects toad maggot essential oil;
(5) essential oil packing.
The isolating toad maggot of this embodiment essential oil concentration reaches 96%.
Claims (1)
1. the separating technology of a toad maggot essential oil is characterized in that said separating technology is:
(1) the dried toad maggot of 1000g is packed in the supercritical carbon dioxide extraction apparatus, is that 10～30Mpa, temperature are that 20～50 ℃, flow are to extract 2～6h under the condition of 10～100L/min at pressure;
(2) supercritical carbon dioxide extraction apparatus pressure is reduced to 7～8Mpa, and temperature is to resolve under 30～35 ℃ of conditions, collects extraction liquid;
(3) extraction liquid is added to the abundant down 10～60min of stirring of normal temperature condition in the sherwood oil, adds 500～2000g atlapulgite again to stir, and normal temperature condition held 6～24h separates under 5000～15000r/min condition with whizzer, collects vat liquor;
(4) vat liquor is to heat 30～80 ℃ under the condition of 0.5～3Mpa in vacuum tightness, and sherwood oil is sloughed in evaporation, collects toad maggot essential oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101786050A CN102676292A (en) | 2012-06-02 | 2012-06-02 | Separation process of toad maggot essential oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101786050A CN102676292A (en) | 2012-06-02 | 2012-06-02 | Separation process of toad maggot essential oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102676292A true CN102676292A (en) | 2012-09-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101786050A Pending CN102676292A (en) | 2012-06-02 | 2012-06-02 | Separation process of toad maggot essential oil |
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| CN (1) | CN102676292A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113509493A (en) * | 2020-04-09 | 2021-10-19 | 大连麦琪克生物技术有限公司 | Bacteriostatic ointment for promoting wound healing and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617787A (en) * | 2009-07-27 | 2010-01-06 | 中国科学院西北高原生物研究所 | Process for continuously extracting a plurality of products from highland barley |
| CN101669892A (en) * | 2009-09-28 | 2010-03-17 | 中山大学 | Arborvitae mask and preparation method thereof |
| CN102078592A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotics in preparing drugs for treating acnes |
| CN102078593A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotic for preparing medicines for curing diabetic foot |
-
2012
- 2012-06-02 CN CN2012101786050A patent/CN102676292A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617787A (en) * | 2009-07-27 | 2010-01-06 | 中国科学院西北高原生物研究所 | Process for continuously extracting a plurality of products from highland barley |
| CN101669892A (en) * | 2009-09-28 | 2010-03-17 | 中山大学 | Arborvitae mask and preparation method thereof |
| CN102078592A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotics in preparing drugs for treating acnes |
| CN102078593A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotic for preparing medicines for curing diabetic foot |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113509493A (en) * | 2020-04-09 | 2021-10-19 | 大连麦琪克生物技术有限公司 | Bacteriostatic ointment for promoting wound healing and preparation method thereof |
| CN113509493B (en) * | 2020-04-09 | 2024-01-30 | 大连麦琪克生物技术有限公司 | Antibacterial ointment for promoting wound healing and preparation method thereof |
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Application publication date: 20120919 |