CN101492484A - Synthetic circulation production process for guanine nucleoside - Google Patents

Abstract

The invention provides a comprehensive recycling production process of guanosine. The bacterial suspension collected from the process in which guanosine is extracted and refined from guanosine fermented liquid is dried as feed additive or made into feed protein; the primary mother liquid acquired from the first four times during micro-filtrate membrane filtration, crude crystallization and pressure filtration and separation is sent into a reverse osmosis membrane for condensation and then mixed with the guanosine fermented liquid for internal recycling; the primary mother liquid acquired from the fifth time undergoes evaporation concentration, crystallization, drying and granulation to produce ammonium sulfate and other bio-compound fertilizers; the secondary mother liquid acquired from ultrafiltration membrane treatment is reused as micro-filtration membrane dialysis water; the third mother liquid acquired from extract crystallization and pressure filtration and separation is respectively reused as anti-crystallinic water of anti-crystallinic jar and dialysis water of micro-filtration membrane and ultrafiltration membrane. The invention overcomes the defects of the traditional process such as incomprehensive utilization, resources waste and environmental pollution; the comprehensive cycling process is reasonable and feasible and conforms to the green environmental protection concept, thus cleaning the environment, promoting the ecological balance, reducing the production cost and strengthening the social and economic benefits.

Description

A kind of synthetic circulation production process of guanosine-

Technical field

The present invention relates to produce the synthetic circulation production process of the production technique of guanosine-, particularly a kind of guanosine-.

Background technology

Guanosine-(guanosine) is the important intermediate of food and medical product, and its purposes is very extensive, and annual requirement is with the speed increase of 10%-20%.At present, the fermentative Production guanosine has been advanced production technique.The refining guanosine of extraction is generally slightly got from the guanosine fermented liquid, essence is got with separate three technological processs, each leaching process all can produce bacteria suspension and residue mother liquor (claiming mother liquor, secondary mother liquid and three mother liquors again a time), these bacteria suspensions and residue mother liquor generally all do not carry out scientific and reasonable comprehensive utilization once more, waste precious resources, polluted environment.

Summary of the invention

At the deficiency of above-mentioned similar technology, the purpose of this invention is to provide a kind of to bacteria suspension and three kinds of guanosine-environmental protection production technique that mother liquor fully utilizes.

Technical scheme of the present invention is:

1, bacteria suspension comprehensive utilization:

Bacteria suspension oven dry with the guanosine fermented liquid produces after microfiltration membrane is handled both can be used as fodder additives, also can be made into feedstuff protein.

2, a mother liquor comprehensive utilization:

1. in the thick leaching process of guanosine, through the guanosine stillness of night of micro-filtrate membrane filtration is stirred decrease temperature crystalline in the crude product crystallizer after, a mother liquor that obtains in the time of will separating with plate-and-frame filter press is sent into reverse osmosis membrane and is concentrated, and then sends into microfiltration membrane and mix with the guanosine fermented liquid and carry out internal recycle technology; 60 ℃ of the temperature of reverse osmosis membrane technology, pressure≤0.35Mpa, flow rate control is at 100L/m ².h-200L/m ².h, be concentrated into guanosine content and reach 15g/L-20g/L;

2. above-mentioned internal recycle technology is one-period with 5 times, and the mother liquor that the 5th obtains is concentrated to 5 times-6 times in temperature≤80 ℃, vacuum pressure under-0.06Mpa--0.08Mpa the condition through quadruple effect evaporator earlier; Again concentrated solution is sent into continuous crystallizer continuous crystallisation under 85 ℃ of temperature, vacuum pressure-0.06Mpa--0.08Mpa condition, obtained inorganic salt crystal such as ammonium sulfate; Separate with continuous conic basket type whizzer again, separating factor 2000, moisture is less than 25%-30%; Under 100 ℃, rotating speed 8r/min-12r/min condition, dry moisture 8%-10% with the tube bank dryer then; With the crystal granulation of tablets press to oven dry, granularity 1.5mm-2.5mm obtains ammonium sulfate and other bio-compound fertilizers at last at last.

3, secondary mother liquid comprehensive utilization:

Removing albumen, pigment through ultra-filtration membrane in the smart leaching process of guanosine, to handle the secondary mother liquid volume that the back obtains less, and pigment, impurity are more, and guanosine content 0.4g/L-0.5g/L can be all utilize as the microfiltration membrane water of dialysing again.

4, three mother liquor comprehensive utilizations:

The guanosine clear liquid of ultrafiltration membrance filter is after the elaboration crystallizer stirs decrease temperature crystalline, three mother liquor volumes that obtain when separating with plate-and-frame filter press are big, pigment, impurity are considerably less, and guanosine content 0.4g/L-0.5g/L can utilize as molten brilliant jar molten brilliant water and microfiltration membrane, ultra-filtration membrane dialysis water respectively again.

Advantage of the present invention is: 1. fully fully utilize the bacteria suspension that obtains in the guanosine production process, mother liquor, secondary mother liquid and three mother liquors, meet the environmental protection new concept, purified environment, promoted the eubiosis; 2. resource effectively comprehensive utilization comprehensively on the basis that guanosine is produced, has been derived from fodder additives, feedstuff protein and composite bio-fertilizer addition product; 3. comprehensive circulation technology is reasonable, feasible, has reduced production cost, has strengthened the corporate social economic benefit.

Embodiment

Concrete production process of the present invention is:

1, bacteria suspension comprehensive utilization:

Bacteria suspension oven dry with the guanosine fermented liquid produces after microfiltration membrane is handled both can be used as fodder additives, also can be made into feedstuff protein.

2, a mother liquor comprehensive utilization:

1. in the thick leaching process of guanosine, through the guanosine stillness of night of micro-filtrate membrane filtration is stirred decrease temperature crystalline in the crude product crystallizer after, a mother liquor that obtains in the time of will separating with plate-and-frame filter press is sent into reverse osmosis membrane and is concentrated, and then sends into microfiltration membrane and mix with the guanosine fermented liquid and carry out internal recycle technology; 60 ℃ of the temperature of reverse osmosis membrane technology, pressure≤0.35Mpa, flow rate control is at 100L/m ².h-200L/m ².h, be concentrated into guanosine content and reach 15g/L-20g/L;

2. above-mentioned internal recycle technology is one-period with 5 times, and the mother liquor that the 5th obtains is concentrated to 5 times-6 times in temperature≤80 ℃, vacuum pressure under-0.06Mpa--0.08Mpa the condition through quadruple effect evaporator earlier; Again concentrated solution is sent into continuous crystallizer in 85 ℃ of temperature, vacuum pressure for continuous crystallisation under the-0.06Mpa--0.08Mpa condition, obtain inorganic salt crystal such as ammonium sulfate; Separate with continuous conic basket type whizzer again, separating factor 2000, moisture is less than 25%-30%; Under 100 ℃, rotating speed 8r/min-12r/min condition, dry moisture 8%-10% with the tube bank dryer then; With the crystal granulation of blade type tablets press to oven dry, granularity 1.5mm-2.5mm obtains ammonium sulfate and other bio-compound fertilizers at last at last.

3, secondary mother liquid comprehensive utilization:

Removing albumen, pigment through ultra-filtration membrane in the smart leaching process of guanosine, to handle the secondary mother liquid volume that the back obtains less, and pigment, impurity are more, and guanosine content 0.4g/L-0.5g/L can be all utilize as the microfiltration membrane water of dialysing again.

4, three mother liquor comprehensive utilizations:

The guanosine clear liquid of ultrafiltration membrance filter is after the elaboration crystallizer stirs decrease temperature crystalline, three mother liquor volumes that obtain when separating with plate-and-frame filter press are big, pigment, impurity are considerably less, and guanosine content 0.4g/L-0.5g/L can utilize as molten brilliant jar molten brilliant water and microfiltration membrane, ultra-filtration membrane dialysis water respectively again.

Claims (1)

1, a kind of synthetic circulation production process of guanosine-is characterized in that:

(1), bacteria suspension comprehensive utilization:

Bacteria suspension oven dry with the guanosine fermented liquid produces after microfiltration membrane is handled both can be used as fodder additives, also can be made into feedstuff protein;

(2), a mother liquor comprehensive utilization:

1. in the thick leaching process of guanosine, through the guanosine clear liquid of micro-filtrate membrane filtration stirs decrease temperature crystalline in the crude product crystallizer after, a mother liquor that obtains in the time of will separating with plate-and-frame filter press is sent into reverse osmosis membrane and is concentrated, and then sends into microfiltration membrane and mix with the guanosine fermented liquid and carry out internal recycle technology; 60 ℃ of the temperature of reverse osmosis membrane technology, pressure≤0.35Mpa, flow rate control is at 100L/m ².h-200L/m ².h, be concentrated into guanosine content and reach 15g/L-20g/L;

2. above-mentioned internal recycle technology is one-period with 5 times, and the mother liquor that the 5th obtains is concentrated to 5 times-6 times in temperature≤80 ℃, vacuum pressure under-0.06Mpa--0.08Mpa the condition through quadruple effect evaporator earlier; Again concentrated solution is sent into continuous crystallizer in 85 ℃ of temperature, vacuum pressure for continuous crystallisation under the-0.06Mpa--0.08Mpa condition, obtain inorganic salt crystal such as ammonium sulfate; Separate with continuous conic basket type whizzer again, separating factor 2000, moisture is less than 25%-30%; Under 100 ℃, rotating speed 8r/min-20r/min condition, dry moisture 8%-10% with the tube bank dryer then; With the crystal granulation of tablets press to oven dry, granularity 1.5mm-2.5mm obtains ammonium sulfate and other bio-compound fertilizers at last at last;

(3), secondary mother liquid comprehensive utilization:

Removing albumen, pigment through ultra-filtration membrane in the smart leaching process of guanosine, to handle the secondary mother liquid volume that the back obtains less, and pigment, impurity are more, and guanosine content 0.4g/L-0.5g/L can be all utilize as the microfiltration membrane water of dialysing again;

(4), three mother liquor comprehensive utilizations:

The guanosine clear liquid of ultrafiltration membrance filter is after the elaboration crystallizer stirs decrease temperature crystalline, three mother liquor volumes that obtain when separating with plate-and-frame filter press are big, pigment, impurity are considerably less, and guanosine content 0.4g/L-0.5g/L can utilize as molten brilliant jar molten brilliant water and microfiltration membrane, ultra-filtration membrane dialysis water respectively again.