CN102675480A - Preparation method of pleurotus nebrodensis polysaccharide with high yield - Google Patents
Preparation method of pleurotus nebrodensis polysaccharide with high yield Download PDFInfo
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- CN102675480A CN102675480A CN2012101547562A CN201210154756A CN102675480A CN 102675480 A CN102675480 A CN 102675480A CN 2012101547562 A CN2012101547562 A CN 2012101547562A CN 201210154756 A CN201210154756 A CN 201210154756A CN 102675480 A CN102675480 A CN 102675480A
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Abstract
The invention discloses a preparation method of pleurotus nebrodensis polysaccharide with high yield, and the method comprises the following steps of: cleaning fresh pleurotus nebrodensis, adding water according to the condition that the mass ratio of pleurotus nebrodensis and water is (1:5)-(1:15), crushing, and sizing; then adjusting the pH to 5-8 with diluted hydrochloric acid or a NaOH solution, cooking for 0.5-2 hours in a closed container at 110-130 DEG C and cooling to room temperature; performing suction filtration or centrifugal separation to obtain a polysaccharide solution; finally, purifying with ethanol, performing centrifugal separation and drying to obtain pleurotus nebrodensis polysaccharide, wherein the yield of pleurotus nebrodensis polysaccharide calculated by weight percentage is 28.9-37.2%. The method for preparing pleurotus nebrodensis polysaccharide is high in production efficiency, and meanwhile ensures that active polysaccharide is fully released to be beneficial to the absorption by a human body. The pleurotus nebrodensis polysaccharide obtained by the invention can be widely applied to fields of food, beverage, health care food and pharmaceuticals.
Description
Technical field
The present invention relates to the preparation technology of Pleurotus nebrodensis polysaccharide, particularly relate to a kind of preparation method who utilizes pyroprocessing to prepare high yield Pleurotus nebrodensis polysaccharide, the Pleurotus nebrodensis polysaccharide of preparation can be widely used in food, beverage, protective foods and pharmaceuticals field.
Background technology
Pleurotus nebrodensis (Pleurotus eryngiivar.nebrodensis) is claimed Pleurotus eryngii var. nebrodensis again, is under the jurisdiction of Agaricales, Pleurotaceae, pleurotus, is the eryngo white mutation of picking up the ears, and, likeness in form glossy ganoderma pure white because of its mushroom body gained the name.Plump, fine and smooth tender and crisp, the delicious flavour of its meat, stature is big, mouthfeel good, commodity is strong, is the rare edible mushrooms of another high-quality after needle mushroom, mushroom, Hericium erinaceus (Bull. Ex Fr.) Pers. and dictyophora phalloidea.According to one's analysis; Pleurotus nebrodensis contains protein 14.7%; Fat 4.31%, robust fibre 15.4%, total amino acid content are 10.6%; Containing multiple mineral element such as linolic acid, unsaturated fatty acids, polyose, calcium, iron, zinc, manganese and abundant vitamins C, E etc. in addition, is a kind of edible and all very high rare edible mushrooms of pharmaceutical use.Contained abundant fungus polysaccharide has enhancing human immune, regulates the effect of human body physiological equilibrium.In very great demand on the market at home and abroad, belong to one of the world ten your name mushrooms, be environmental protective hygienic food, DEVELOPMENT PROSPECT is boundless.Albumen and polysaccharide content are higher in the Pleurotus nebrodensis, and system development in addition can be processed various nutritious prod; Seasonings, drink additive and anti-cancer, cancer therapy drug, it is the high novel product of a kind of economic worth surely; High economic benefit and social benefit are arranged, and development prospect will be very wide.
The extraction of polysaccharide will be adopted different process for extracting according to the existence form and the extract part difference of polysaccharide.As a whole, though methods such as traditional acid is carried, alkali is carried, hot water lixiviate all are to extract the classical way of polysaccharide, the hot water extraction time is long, efficient is low; The polysaccharide soln viscosity of alkaline extraction gained is too big, filtration difficulty, and the three-dimensional optically active structure of polysaccharide had certain destruction, influence its physiologically active; The acid formulation possibly have certain destruction to polysaccharide component equally, and bigger to the corrodibility of container.Therefore, explore more efficient, economic, reliable process for extracting and have realistic meaning.Adopt stripping that pyroprocessing under the neutrallty condition will help its glucide and separate, thereby improve the extraction yield of polysaccharide.
Chinese invention patent application 201110204035.3 discloses the optimization preparation method of Pleurotus nebrodensis polysaccharide, and the Pleurotus nebrodensis polysaccharide is as the purposes of wetting Agent for Printing Inks and use the makeup of Pleurotus nebrodensis polysaccharide as wetting Agent for Printing Inks.The optimization preparation method of this Pleurotus nebrodensis polysaccharide is: a) the Pleurotus nebrodensis raw material is added water, smash laggard action attitude circulated in countercurrent to pieces and extract; B) step a) gained extracting solution is decoloured with aluminum oxide, be concentrated into relative density of medicine liquid 1.05-1.10, add ethanol to producing white flocks; This deposition is the Pleurotus nebrodensis polysaccharide.In day cream, late frost, facial mask, the dew of preserving moisture, add 0.1%-2% Pleurotus nebrodensis polysaccharide, have tangible moisture-keeping function.This method need be decoloured with aluminum oxide, and polysaccharide component is had certain destruction, and the yield of this method Pleurotus nebrodensis polysaccharide is not high.
Summary of the invention
The object of the invention overcomes the shortcoming of prior art, provides a kind of very little to Pleurotus nebrodensis polysaccharide destructiveness, and the preparation method of the Pleurotus nebrodensis polysaccharide of high yield, makes and can all discharge by active polysaccharide, helps absorption of human body.
The present invention cleans fresh Pleurotus nebrodensis, adds water in proportion, fragmentation, and making beating, with Hydrogen chloride or NaOH regulation system pH, the high temperature steaming postcooling separates to room temperature, obtains Crude polysaccharides solution, is further purified processing again, is drying to obtain the Pleurotus nebrodensis polysaccharide.
The object of the invention is realized through following technical scheme:
A kind of preparation method with Pleurotus nebrodensis polysaccharide of high yield comprises the steps and processing condition:
(1) fresh Pleurotus nebrodensis being cleaned, is that 1:5-1:15 adds water by the mass ratio of Pleurotus nebrodensis and water, and fragmentation is sized mixing;
(2) with Hydrogen chloride or NaOH solution regulation system pH to 5-8, in encloses container 110-130 ℃ boiling 0.5-2 hour, be cooled to room temperature;
(3) suction filtration or spinning obtain Crude polysaccharides solution;
(4) handle with ethanol purification, spinning is drying to obtain the Pleurotus nebrodensis polysaccharide, and by percentage to the quality, the Pleurotus nebrodensis polysaccharide yield is 28.9%-37.2%.
Be further to realize that it is that to use the mass concentration of 4 times of volumes earlier be that 99.5% ethanol purification is handled that the object of the invention, said ethanol purification are handled, and then to use 3 times of volume mass concentration be that 80% ethanol purification is handled.
Said drying is preferably spraying drying, warm air drying or vacuum lyophilization.
Said encloses container is preferably the encloses container of high temperature high voltage resistant.
Described cooking time is preferably 1-2 hour.
Said stewing temperature is preferably 115-125 ℃.
With respect to prior art, the present invention has following advantage and beneficial effect:
(1) the inventive method helps the leaching of polysaccharide exceeding boiling under 100 ℃ the high temperature; Carry out under the airtight environment; Do not have the volatilization of solvent, the raising of temperature can appropriate cause the fracture of glycosidic link and can not change the chemical structure of Pleurotus nebrodensis polysaccharide, so soluble polysaccharide content can be significantly improved; By percentage to the quality, the Pleurotus nebrodensis polysaccharide yield is 28.9%-37.2%.This technology meets " green " environmental protection requirement.
(2) the hot water microwave leaching technology extraction time weak point that the inventive method is traditional relatively; Reduce the stripping of impurity component, improved the yield of effective constituent, the Pleurotus nebrodensis polysaccharide lighter color that obtains; Solvability is good, in the food that can well add, healthcare products and the pharmaceutical industries.
(3) owing to reason such as culture environment condition and the particular requirement of Pleurotus nebrodensis physiology be incompatible; Some Pleurotus nebrodensis misshapen mushrooms or secondary mushroom have been produced, like mushroom body atrophy shape, the inclined to one side xanthiochromatic of mushroom body etc.; Reduced product hierarchy; Even lose commodity value, and also not relating to for the Application and Development of Pleurotus nebrodensis waste is at present domestic, this is the significant wastage on a kind of resource.The inventive method has improved the added value of misshapen mushroom, secondary mushroom, has also alleviated the fresh-keeping burden of Pleurotus nebrodensis simultaneously well.
Description of drawings
Fig. 1 is the light absorption value canonical plotting of different glucose concn.
Embodiment
Below in conjunction with embodiment the present invention is further specified, but the scope that the present invention requires to protect is not limited to the scope that embodiment expresses.
Get 100g Pleurotus nebrodensis misshapen mushroom, add the water of 5 times of quality, fragmentation; Making beating, using mass concentration is 0.5% Hydrogen chloride regulation system pH to 5.0, under 120 ℃ the temperature; Boiling is 1 hour in autoclave, and rotating speed is spinning under the condition of 8000g, is cooled to room temperature; Obtain Crude polysaccharides solution, the mass concentration of using 4 times of volumes is that 99.5% ethanol purification is handled, and then to use the mass concentration of 3 times of volumes be that 80% ethanol purification is handled; Rotating speed is spinning under the condition of 5000g, and spraying drying promptly obtains Pleurotus nebrodensis polysaccharide 35.8g, product colour white.Get the Pleurotus nebrodensis polysaccharide 25mg that spraying drying obtains and in the 250mL volumetric flask, add water to scale marks, shake up, draw 1.0mL then in test tube; Add water and mend, add mass concentration then lentamente and be 6% the phenol liquid 1.0mL and the 5.0mL vitriol oil, left standstill 10 minutes to 2.0mL; Shake up, room temperature was placed 20 minutes, was contrast with zero(ppm) water; Survey light absorption value in the 495nm place, calculate total sugar content 85.11% in the Pleurotus nebrodensis polysaccharide.Method of calculation and result see table 1,2.
The comparative example 1
Get 100g Pleurotus nebrodensis misshapen mushroom, add the water of 5 times of quality, fragmentation; Making beating, using mass concentration is 0.5% Hydrogen chloride regulation system pH to 5.0, under 85 ℃ the temperature; Hot water lixiviate 6h, rotating speed are spinning under the condition of 8000g, are cooled to room temperature; Obtain Crude polysaccharides solution, the mass concentration of using 4 times of volumes is that 99.5% ethanol purification is handled, and then to use the mass concentration of 3 times of volumes be that 80% ethanol purification is handled; Rotating speed is spinning under the condition of 5000g, and spraying drying promptly obtains Pleurotus nebrodensis polysaccharide 9.38g.Product colour is yellow.Get the Pleurotus nebrodensis polysaccharide 25mg that spraying drying obtains and in the 250mL volumetric flask, add water to scale marks, shake up, draw 1.0mL then in test tube; Add water and mend, add mass concentration then lentamente and be 6% the phenol liquid 1.0mL and the 5.0mL vitriol oil, left standstill 10 minutes to 2.0mL; Shake up, room temperature was placed 20 minutes, was contrast with zero(ppm) water; Survey light absorption value in the 495nm place, calculate total sugar content 76.26% in the Pleurotus nebrodensis polysaccharide.
Embodiment 2
Get 100g and pluck the remaining Pleurotus nebrodensis root in back, add the water of 10 times of quality, fragmentation, making beating; With 0.5%NaOH solution regulation system pH to 8.0, under 130 ℃ the temperature, boiling is 0.5 hour in autoclave, suction filtration; Be cooled to room temperature, obtain Crude polysaccharides solution, the mass concentration of using 4 times of volumes is 99.5% ethanol purification processing; And then to use 3 times of volume mass concentration be that 80% ethanol purification is handled, and rotating speed is spinning under the condition of 5000g, and lyophilize promptly obtains Pleurotus nebrodensis polysaccharide 28.9g.Get the Pleurotus nebrodensis polysaccharide 25mg that spraying drying obtains and in the 250mL volumetric flask, add water to scale marks, shake up, draw 1.0mL then in test tube; Add water and mend, add mass concentration then lentamente and be 6% the phenol liquid 1.0mL and the 5.0mL vitriol oil, left standstill 10 minutes to 2.0mL; Shake up, room temperature was placed 20 minutes, was contrast with zero(ppm) water; Survey light absorption value in the 495nm place, calculate total sugar content 79.26% in the Pleurotus nebrodensis polysaccharide.Method of calculation and result see table 1,2.
Embodiment 3
Get 100g broken old edge part of Pleurotus nebrodensis or handle, add the water of 15 times of quality, fragmentation; Making beating is with 0.5%NaOH solution regulation system pH to 7.0, under 110 ℃ the temperature; Boiling is 2 hours in the encloses container autoclave of high temperature high voltage resistant, and rotating speed is spinning under the 8000g condition, is cooled to room temperature; Obtain Crude polysaccharides solution, the mass concentration of using 4 times of volumes is that 99.5% ethanol purification is handled, and then to use the mass concentration of 3 times of volumes be that 80% ethanol purification is handled; Rotating speed is spinning under the 5000g condition, and 60 ℃ of warm air drying 5h promptly obtain Pleurotus nebrodensis polysaccharide 37.2g.Get the Pleurotus nebrodensis polysaccharide 25mg that spraying drying obtains and in the 250mL volumetric flask, add water to scale marks, shake up, draw 1.0mL then in test tube; Add water and mend, add mass concentration lentamente and be 6% the phenol liquid 1.0mL and the 5.0mL vitriol oil, left standstill 10 minutes to 2.0mL; Shake up, room temperature was placed 20 minutes, was contrast with zero(ppm) water; Survey light absorption value in the 495nm place, calculate total sugar content 81.45% in the Pleurotus nebrodensis polysaccharide.Method of calculation and result see table 1,2.
The present invention adopts the phenolsulfuric acid method to measure total sugar content.
Phenol-sulfuric acid process is to measure the domestic method of polysaccharide.Its ultimate principle is: select for use ethanol to remove interference components such as monose in the sample, oligose, use polyose composition contained in the deionized water extraction sample then.Polysaccharide is hydrolyzed into monose under vitriol oil effect, and dehydration generates furfural derivatives rapidly, is condensed into colored compound with phenol, uses its polysaccharide content of colorimetric method for determining then.
The making of typical curve: accurately take by weighing 25mg glucose and in the 250mL volumetric flask, add water to scale marks, shake up, draw 0.4,0.6,0.8,1.0,1.2,1.4,1.6,1.8 (mL) then respectively in 8 test tubes; Respectively add water and mend, add mass concentration then lentamente and be 6% the phenol liquid 1.0mL and the 5.0mL vitriol oil, left standstill 10 minutes to 2.0mL; Shake up, room temperature was placed 20 minutes, was contrast with zero(ppm) water; Surveying light absorption value in the 495nm place, is X-coordinate with the glucose concn, and light absorption value is an ordinate zou; The drawing standard curve, shown in Figure 1.Wherein y is function (a dependent variable light absorption value) in this linear equation, and x is independent variable(s) (glucose concn), and R is a linearly dependent coefficient.R
2=0.9971 shows that this linear equation highly linear is relevant.The measuring result lists table 1 in.
The mensuration table of table 1 glucose typical curve
| Glucose concn C (μ g) | 40 | 60 | 80 | 100 | 120 | 140 | 160 | 180 |
| Absorbance A | 0.312 | 0.481 | 0.607 | 0.798 | 0.931 | 1.096 | 1.221 | 1.340 |
The mensuration of total sugar content in the Pleurotus nebrodensis polysaccharide: adopt the phenolsulfuric acid method to measure total sugar content, article with glucose as a standard, 25mg adds water to scale marks in the 250mL volumetric flask with the Pleurotus nebrodensis polysaccharide; Shake up, draw 1.0mL then in test tube, add water and mend to 2.0mL; Add mass concentration then lentamente and be 6% the phenol liquid 1.0mL and the 5.0mL vitriol oil, left standstill 10 minutes, shake up; Room temperature was placed 20 minutes, was contrast with zero(ppm) water, surveyed light absorption value in the 495nm place; The reference standard curve calculates total sugar content in the Pleurotus nebrodensis polysaccharide, and is specifically as shown in table 2.
Calculation formula:
Total reducing sugar percentage composition=(measuring concentration X extension rate X extracting liquid volume)/Pleurotus nebrodensis polysaccharide quality X100%;
The matter of Pleurotus nebrodensis heavy (g) before yield=products obtained therefrom quality (g)/extraction.
Total sugar content table in the Pleurotus nebrodensis polysaccharide among table 2 embodiment
Claims (6)
1. preparation method with Pleurotus nebrodensis polysaccharide of high yield is characterized in that comprising the steps and processing condition:
(1) fresh Pleurotus nebrodensis being cleaned, is that 1:5-1:15 adds water by the mass ratio of Pleurotus nebrodensis and water, and fragmentation is sized mixing;
(2) with Hydrogen chloride or NaOH solution regulation system pH to 5-8, in encloses container 110-130 ℃ boiling 0.5-2 hour, be cooled to room temperature;
(3) suction filtration or spinning obtain Crude polysaccharides solution;
(4) handle with ethanol purification, spinning is drying to obtain the Pleurotus nebrodensis polysaccharide, and by percentage to the quality, the Pleurotus nebrodensis polysaccharide yield is 28.9%-37.2%.
2. the preparation method with Pleurotus nebrodensis polysaccharide of high yield according to claim 1; It is characterized in that: it is that to use the mass concentration of 4 times of volumes earlier be that 99.5% ethanol purification is handled that said ethanol purification is handled, and then to use 3 times of volume mass concentration be that 80% ethanol purification is handled.
3. the preparation method with Pleurotus nebrodensis polysaccharide of high yield according to claim 1 is characterized in that: said drying is spraying drying, warm air drying or vacuum lyophilization.
4. the preparation method with Pleurotus nebrodensis polysaccharide of high yield according to claim 1 is characterized in that: said encloses container is the encloses container of high temperature high voltage resistant.
5. the preparation method with Pleurotus nebrodensis polysaccharide of high yield according to claim 1 is characterized in that: described cooking time is 1-2 hour.
6. the preparation method with Pleurotus nebrodensis polysaccharide of high yield according to claim 1 is characterized in that: said stewing temperature is 115-125 ℃.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104744601A (en) * | 2015-04-10 | 2015-07-01 | 新疆大学 | Method for extracting and purifying fleurotus ferulae polysaccharide |
| CN107927481A (en) * | 2017-12-15 | 2018-04-20 | 佛山实瑞先导材料研究院（普通合伙） | A kind of teenager's maltose nutritious drink and preparation method thereof |
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| JPH07135847A (en) * | 1993-11-17 | 1995-05-30 | Mizushi Fujimoto | Additive to culture medium for edible fungi |
| CN101463093A (en) * | 2007-12-17 | 2009-06-24 | 天津市华泰森淼生物工程技术有限公司 | Technique for extracting polysaccharide from Phellinus linteus |
| CN102241784A (en) * | 2010-05-10 | 2011-11-16 | 曹传圣 | Extraction method for preparing polysaccharide from straw mushrooms |
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2012
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07135847A (en) * | 1993-11-17 | 1995-05-30 | Mizushi Fujimoto | Additive to culture medium for edible fungi |
| CN101463093A (en) * | 2007-12-17 | 2009-06-24 | 天津市华泰森淼生物工程技术有限公司 | Technique for extracting polysaccharide from Phellinus linteus |
| CN102241784A (en) * | 2010-05-10 | 2011-11-16 | 曹传圣 | Extraction method for preparing polysaccharide from straw mushrooms |
Non-Patent Citations (1)
| Title |
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| 马淑凤等: "白灵菇多糖研究进展", 《安徽农业科学》, vol. 33, no. 7, 30 July 2005 (2005-07-30) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104744601A (en) * | 2015-04-10 | 2015-07-01 | 新疆大学 | Method for extracting and purifying fleurotus ferulae polysaccharide |
| CN104744601B (en) * | 2015-04-10 | 2017-02-01 | 新疆大学 | Method for extracting and purifying fleurotus ferulae polysaccharide |
| CN107927481A (en) * | 2017-12-15 | 2018-04-20 | 佛山实瑞先导材料研究院（普通合伙） | A kind of teenager's maltose nutritious drink and preparation method thereof |
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