CN102675409A - Collecting liquid capable of keeping activity of maggot secretion excrements - Google Patents
Collecting liquid capable of keeping activity of maggot secretion excrements Download PDFInfo
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- CN102675409A CN102675409A CN2012101232702A CN201210123270A CN102675409A CN 102675409 A CN102675409 A CN 102675409A CN 2012101232702 A CN2012101232702 A CN 2012101232702A CN 201210123270 A CN201210123270 A CN 201210123270A CN 102675409 A CN102675409 A CN 102675409A
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- excrements
- secretion
- worm
- buffer solution
- phosphate buffer
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a collecting liquid capable of keeping the activity of maggot secretion excrements, which is phosphate buffer solution of Tween80 or phosphate buffer solution of serum albumin, the mass-volume ratio of the Tween80 to the phosphate buffer solution is 10 gram/liter, and the mass-volume ratio of the serum albumin to the phosphate buffer solution is 1 gram/liter. The collecting liquid has the benefits that biological active components of the collected maggot secretion excrements can be kept stable, the quality of collected matters is ensured, the maggot secretion excrements can be still kept in stability even if the temperature is more largely changed (the number of the repeated freezing and thawing times reaches 10) and can be saved for a long time, and the clinical application of the maggot secretion excrements and the further research on treatment efficacy are facilitated.
Description
Technical field
The present invention relates to a kind of collection worm of all sorts grains and secrete excremental collection liquid, especially a kind of stability that keeps secreting bioactive ingredients in the movement, the worm of all sorts grains that is beneficial to preservation secretes excremental collection liquid.
Background technology
Promote wound healing because worm of all sorts grains (maggot) can engulf/gnaw slough, the worm of all sorts grains live body is used to treat the necrosis wound serious with infection that causes because of wound for a long time for this reason.At present, people have found to contain the large number of biological activeconstituents in the secretory product and movement in the five cereals insect population, and like protein and polypeptide compounds etc., these materials all play a significant role at aspects such as antibiotic, anti-inflammatory and short cell proliferations.Existing collect worm of all sorts grains and secrete excremental method and be: the worm of all sorts grains that will clean put into saline water (20/10ml) about 5 ~ 10 minutes, it spued and drain substance in vivo.Be difficult to keep protein and the stability of other biologically active substance in the worm of all sorts grains secretion movement with saline water as collecting liquid; Thereby influenced collected worm of all sorts grains secretion movement total quality and be unfavorable for prolonged preservation, restricted the further research that worm of all sorts grains secretes excremental clinical application and therapeutic efficiency.
Summary of the invention
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, a kind of stability that keeps secreting bioactive ingredients in the movement to be provided, and the worm of all sorts grains that is beneficial to preservation secretes excremental collection liquid.
Technical solution of the present invention is: a kind of active collection liquid of worm of all sorts grains secretion movement that keeps; Be the phosphate buffered saline buffer of Tween 80 or the phosphate buffered saline buffer of bovine serum albumin; Tween 80 is 10 grams per liters with the mass volume ratio of phosphate buffered saline buffer, and the mass volume ratio of bovine serum albumin and phosphate buffered saline buffer is 1 grams per liter.
The present invention compared with prior art; The stability that the worm of all sorts grains that can keep collected secretes the movement bioactive ingredients; Guaranteed the quality of gleanings; Even under the situation of the bigger variation of temperature appearance (multigelation reaches surplus in the of 10 inferior), still can keep its stability, but prolonged preservation is beneficial to the further research that worm of all sorts grains secretes excremental clinical application and therapeutic efficiency.
Description of drawings
Fig. 1 is the gel electrophoresis figure of the embodiment of the invention and prior art.
Embodiment
Embodiment 1:
10 gram Tween 80 are dissolved in 1 liter of phosphate buffered saline buffer, promptly make collection liquid.
Said Tween 80 and phosphate buffered saline buffer (PBS) are prior art.
The same prior art of collection process, the worm of all sorts grains that is about to clean put into present embodiment 1 collect liquid (20/10ml) about 5 ~ 10 minutes, take out worm of all sorts grains, then collect and contain worm of all sorts grains in the liquid and spue and excretory secretion movement.
Embodiment 2:
1 gram bovine serum albumin (BSA) powder is dissolved in 1 liter of phosphate buffered saline buffer, promptly makes collection liquid.
Said bovine serum albumin (BSA) powder and phosphate buffered saline buffer (PBS) are prior art.
The same prior art of collection process, the worm of all sorts grains that is about to clean put into present embodiment 2 collect liquid (20/10ml) about 5 ~ 10 minutes, take out worm of all sorts grains, then collect and contain worm of all sorts grains in the liquid and spue and excretory secretion movement.
Experiment:
Liquid is resulting to be contained worm of all sorts grains and secretes excremental gleanings in order to collect to get the embodiment of the invention 1, embodiment 2 and prior art (saline water); Under equal conditions carry out gel electrophoresis behind the multigelation 10 times, electrophorogram is as shown in Figure 1: 1 is the embodiment of the invention 1; 2 is the embodiment of the invention 2; 3 is prior art.The result shows: the protein ingredient among embodiment 1, the embodiment 2 promptly is under multigelation reaches 10 times situation, also still can keep stable, the then most of degraded of protein liquid of the prior art.Therefore, adopt the worm of all sorts grains secretion movement of the present invention's preparation very to be fit to use, prolonged preservation and long-distance transport under the various conditions.
Claims (1)
1. one kind can keep the active collection liquid of worm of all sorts grains secretion movement; It is characterized in that it being the phosphate buffered saline buffer of Tween 80 or the phosphate buffered saline buffer of bovine serum albumin; Tween 80 is 10 grams per liters with the mass volume ratio of phosphate buffered saline buffer, and the mass volume ratio of bovine serum albumin and phosphate buffered saline buffer is 1 grams per liter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101232702A CN102675409A (en) | 2012-04-25 | 2012-04-25 | Collecting liquid capable of keeping activity of maggot secretion excrements |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101232702A CN102675409A (en) | 2012-04-25 | 2012-04-25 | Collecting liquid capable of keeping activity of maggot secretion excrements |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102675409A true CN102675409A (en) | 2012-09-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101232702A Pending CN102675409A (en) | 2012-04-25 | 2012-04-25 | Collecting liquid capable of keeping activity of maggot secretion excrements |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102675409A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1612756A (en) * | 2001-11-17 | 2005-05-04 | 英国国防部 | Composition and method for treatment of wounds |
| CN101448532A (en) * | 2006-05-17 | 2009-06-03 | Agt科学研究有限公司 | Delivery means |
| CN101541829A (en) * | 2006-05-31 | 2009-09-23 | 英国国防部 | Chymotrypsin from lucilia sericata larvae and its use for the treatment of wounds |
| CN102388135A (en) * | 2009-03-03 | 2012-03-21 | B.R.A.I.N.生物技术研究和信息网络公司 | Protease for wound conditioning and skin care |
-
2012
- 2012-04-25 CN CN2012101232702A patent/CN102675409A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1612756A (en) * | 2001-11-17 | 2005-05-04 | 英国国防部 | Composition and method for treatment of wounds |
| CN101448532A (en) * | 2006-05-17 | 2009-06-03 | Agt科学研究有限公司 | Delivery means |
| CN101541829A (en) * | 2006-05-31 | 2009-09-23 | 英国国防部 | Chymotrypsin from lucilia sericata larvae and its use for the treatment of wounds |
| CN102388135A (en) * | 2009-03-03 | 2012-03-21 | B.R.A.I.N.生物技术研究和信息网络公司 | Protease for wound conditioning and skin care |
Non-Patent Citations (2)
| Title |
|---|
| 林爱华等: "表面活性剂对蛋白质变性作用的研究", 《中南民族大学学报(自然科学版)》, vol. 21, no. 1, 30 March 2002 (2002-03-30) * |
| 王辉: "蛋白制剂中Tween80 含量检测研究", 《河北化工》, vol. 34, no. 3, 20 March 2011 (2011-03-20) * |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120919 |