CN102645494A - Internal heating device for clinical sample machine of glycosylated hemoglobin analyzer - Google Patents
Internal heating device for clinical sample machine of glycosylated hemoglobin analyzer Download PDFInfo
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- CN102645494A CN102645494A CN2012101226877A CN201210122687A CN102645494A CN 102645494 A CN102645494 A CN 102645494A CN 2012101226877 A CN2012101226877 A CN 2012101226877A CN 201210122687 A CN201210122687 A CN 201210122687A CN 102645494 A CN102645494 A CN 102645494A
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- glycosylated hemoglobin
- seat
- heating
- cooling
- heating device
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 53
- 102000017011 Glycated Hemoglobin A Human genes 0.000 title claims abstract description 39
- 108010014663 Glycated Hemoglobin A Proteins 0.000 title claims abstract description 39
- 238000001816 cooling Methods 0.000 claims abstract description 34
- 239000002250 absorbent Substances 0.000 claims description 3
- 238000005452 bending Methods 0.000 claims description 3
- 125000004122 cyclic group Chemical group 0.000 claims description 3
- 230000007704 transition Effects 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 9
- 238000001514 detection method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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Abstract
The invention belongs to medical examination equipment, relates to a component structure of a glycosylated hemoglobin analyzer, and particularly relates to an internal heating device of a clinical sample machine of a glycosylated hemoglobin analyzer. The internal heating device comprises a peltier part, a heating seat is connected to one side of a radiating surface of the peltier part, a cooling seat is connected to one side of a heat absorption surface of the peltier part, a heating tube is disposed on the heating seat, a cooling tube is arranged on the cooling seat, an inlet of the heating tube is connected with a sample liquid supply pipeline of the glycosylated hemoglobin analyzer, an outlet of the heating tube is connected with an inlet of the cooling tube by a transitional tube, and an outlet of the cooling tube leads to a chromatographic column of the glycosylated hemoglobin analyzer. The internal heating device is simple in structure, convenient in operation, reliable in running and high in efficiency.
Description
Technical field
The invention belongs to medical inspection equipment, relate to the modular construction of glycosylated hemoglobin analyser, specifically is a kind of glycosylated hemoglobin analyser clinical sample machine inner heating device.
Background technology
Glycosylated hemoglobin is that haemoglobin generates back and carbohydrate through non-enzymatic be combined into; The terminal positive charge of two β chain N-of the haemoglobin after the saccharification is removed by glycosyl; Positive charge is few than haemoglobin, and therefore, haemoglobin is different to the affinity of Zeo-karb with glycosylated hemoglobin; Positively charged its less absorption affinity a little less than, be easy to eluted earlier.Through the damping fluid of different pH values, just can wash-out separates through the absorption of Zeo-karb with glycosylated hemoglobin with haemoglobin.Adopting the glycosylated hemoglobin analyser of liquid phase chromatography is exactly this characteristic of utilizing glycosylated hemoglobin; Through chromatographic column glycosylated hemoglobin is separated from the haemoglobin of clinical sample, accounted for the percentage of total hemoglobin again through the glycosylated hemoglobin in the photometric determination eluent.
Generate back and carbohydrate through non-enzymatic be combined into because glycosylated hemoglobin is a haemoglobin, its building-up process is slowly, and is irreversible relatively, therefore, in the process that generates, can exist and accomplish the variant that combines.Because the existence of variant, glycosylated hemoglobin can receive the influence of variant and make testing result produce unsettled deviation in the process of wash-out and photometric detection, have a strong impact on the accuracy of detection of glycosylated hemoglobin.
For reducing the influence of glycosylated hemoglobin variant; Generally can eliminate the influence of glycosylated hemoglobin variant through the method for heating, usually sample heated to more than 50 ℃ fast, but too high temperature can make albumen produce sex change again; Therefore, add temperature and need obtain accurate control.And the Zeo-karb that the chromatographic column that is used for separating glycosylated hemoglobin is filled its activity when temperature surpasses more than 30 ℃ will reduce greatly, thereby has a strong impact on absorption, the separation of glycosylated hemoglobin.Therefore, when detecting glycosylated hemoglobin common heating method generally the employing machine add the two kinds of methods of heating in the gentle machine.Machine is heated outward and generally through water-bath or special-purpose heating apparatus sample is warmed to about 50 ℃; After insulation several minutes; With sample place be cooled to the running temperature that allows near chromatographic column naturally under the room temperature environment condition after, again sample is imported in the analyser separate, wash-out and detection.Heating in the machine then is that a heating arrangement and a heat sink are set respectively in the path of instrument internal sample flow warp, is respectively applied for the gentle cooling of adding of sample is handled.Adopt above-mentioned two kinds of methods, all can reduce the influence of variant, but very complicated, troublesome poeration is prone to fault, and efficient is low.
Summary of the invention
Technical matters to be solved by this invention is, a kind of simple in structure, easy to operate, reliable, glycosylated hemoglobin analyser clinical sample machine inner heating device that efficient is high is provided.
Glycosylated hemoglobin analyser clinical sample machine inner heating device of the present invention includes a Peltier, and radiating surface one side of Peltier is connected with the seat of heating, and heat-absorbent surface one side of Peltier is connected with a cooling seat; The seat of heating is provided with one section heating tube, and the cooling seat is provided with one section cooling pipe; The inlet of heating tube is connected with the sample feed flow pipeline of glycosylated hemoglobin analyser, and the outlet of heating tube is connected with the inlet of managing through transition conduit and cooling, the chromatographic column that the glycosylated hemoglobin analyser is led in the outlet of cooling pipe.
Said heating tube is the broken line shape of cyclic bending with the cooling pipe.
First side plate is stamped in the said heating tube outside, and heating tube is clamped and fixed in first side plate and heats between the seat; Cooling pipe arranged outside has second side plate, and the cooling pipe is clamped and fixed between second side plate and the cooling seat.
Advantage of the present invention is:
1, adopts this method, can effectively eliminate in the clinical sample glycosylated hemoglobin variant the influence of accuracy of detection;
2, simple in structure, with the flow path system of a high precision Peltier device, uniqueness, form a complete heat sink again of heating fast, make apparatus structure compact more, operation is conveniently, reliably, efficiently.
3, through the heat sink again of heating, the sample that gets into chromatographic column is more near the working temperature of chromatographic column, makes chromatographic column absorption, to separate glycosylated hemoglobin more effective.
4, because stream is reasonable, effectively reduce the probability of instrument cross pollution.
Description of drawings
Fig. 1 is the main TV structure synoptic diagram of the embodiment of the invention;
Fig. 2 is the right side partial sectional view of Fig. 1;
Fig. 3 is the left side partial sectional view of Fig. 1;
Fig. 4 is the plan structure synoptic diagram of Fig. 1;
Fig. 5 is the flow path system synoptic diagram of the glycosylated hemoglobin analyser of this employing apparatus of the present invention.
Embodiment
As shown in Figure 5; This glycosylated hemoglobin analyser clinical sample machine inner heating device 1 of the present invention is connected between the blood red egg analyser of saccharification sample feed flow pipeline 2 and the chromatographic column 3; Elder generation is through the time that being heated to uniform temperature and maintenance setting of heating tube 14 behind the clinical sample entering heating arrangement; The cooling pipe 15 of process is cooled to the temperature that chromatographic column allows again, flows out heating arrangement then and goes to chromatographic column.
Like Fig. 1-shown in Figure 4, this glycosylated hemoglobin analyser clinical sample machine inner heating device includes a Peltier 11, and radiating surface one side of Peltier is connected with the seat 12 of heating, and heat-absorbent surface one side of Peltier is connected with a cooling seat 13; The seat of heating is provided with one section heating tube 14, and the cooling seat is provided with one section cooling pipe 15; The inlet of heating tube 14 is connected with the sample feed flow pipeline of glycosylated hemoglobin analyser, and the outlet of heating tube 14 is connected the chromatographic column that the glycosylated hemoglobin analyser is led in the outlet of cooling pipe 15 with the inlet of managing 15 through transition conduit 16 and cooling.Heating tube 14 is the broken line shape of cyclic bending with cooling pipe 15.First side plate 17 is stamped in heating tube 14 outsides, and heating tube 14 is clamped and fixed in first side plate 17 and heats between the seat 12; Cooling pipe 15 arranged outside have second side plate 18, and cooling pipe 15 is clamped and fixed between second side plate 18 and the cooling seat 13.
Claims (3)
1. glycosylated hemoglobin analyser clinical sample machine inner heating device; It is characterized in that: it includes a Peltier (11); Radiating surface one side of Peltier is connected with the seat (12) of heating, and heat-absorbent surface one side of Peltier is connected with a cooling seat (13); The seat of heating is provided with one section heating tube (14), and the cooling seat is provided with one section cooling pipe (15); The inlet of heating tube (14) is connected with the sample feed flow pipeline of glycosylated hemoglobin analyser; The outlet of heating tube (14) is connected with the inlet of managing (15) through transition conduit (16) and cooling, and the chromatographic column of glycosylated hemoglobin analyser is led in the outlet of cooling pipe (15).
2. glycosylated hemoglobin analyser clinical sample machine inner heating device according to claim 1 is characterized in that: heating tube (14) and cooling pipe (15) are the broken line shape of cyclic bending.
3. glycosylated hemoglobin analyser clinical sample machine inner heating device according to claim 1; It is characterized in that: first side plate (17) is stamped in heating tube (14) outside, and heating tube (14) is clamped and fixed in first side plate (17) and heats between the seat (12); Cooling pipe (15) arranged outside has second side plate (18), and cooling pipe (15) is clamped and fixed between second side plate (18) and the cooling seat (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210122687 CN102645494B (en) | 2012-04-25 | 2012-04-25 | Internal heating device for clinical sample machine of glycosylated hemoglobin analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210122687 CN102645494B (en) | 2012-04-25 | 2012-04-25 | Internal heating device for clinical sample machine of glycosylated hemoglobin analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102645494A true CN102645494A (en) | 2012-08-22 |
| CN102645494B CN102645494B (en) | 2013-06-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210122687 Expired - Fee Related CN102645494B (en) | 2012-04-25 | 2012-04-25 | Internal heating device for clinical sample machine of glycosylated hemoglobin analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102645494B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106996885A (en) * | 2017-03-24 | 2017-08-01 | 江苏奥迪康医学科技股份有限公司 | Glycolated hemoglobin analysis melts blood pool and its pre-temperature method |
| CN109406693A (en) * | 2018-12-09 | 2019-03-01 | 大连技嘉科技有限公司 | Glycolated hemoglobin analysis column oven |
Citations (8)
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|---|---|---|---|---|
| FR2553194A1 (en) * | 1983-10-05 | 1985-04-12 | Eternit Financiere | Method for fixing flat material samples and device for implementing it |
| CN1412504A (en) * | 2001-10-08 | 2003-04-23 | 中国科学院生态环境研究中心 | Semiconductor refrigerated low-temp. chromatograph enrichment and separation equipment |
| US20070060872A1 (en) * | 2005-02-14 | 2007-03-15 | Hall W D | Apparatus and methods for analyzing body fluid samples |
| CN101292148A (en) * | 2005-08-19 | 2008-10-22 | 加拿大血液中心 | Sample holder for dynamic light scattering |
| JP2010287729A (en) * | 2009-06-11 | 2010-12-24 | Okano Electric Wire Co Ltd | Heating/cooling test apparatus |
| CN202057637U (en) * | 2011-04-06 | 2011-11-30 | 江苏奥迪康医学科技有限公司 | Thermostatic structure of chromatographic column of glycolated hemoglobin analyzer |
| CN202133580U (en) * | 2011-06-28 | 2012-02-01 | 上海惠中医疗科技有限公司 | Heating mechanism for fully automatic glycosylated hemoglobin analyzer |
| CN202599917U (en) * | 2012-04-25 | 2012-12-12 | 江苏奥迪康医学科技有限公司 | Heating device in saccharification haemoglobin analyser clinical sample machine |
-
2012
- 2012-04-25 CN CN 201210122687 patent/CN102645494B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2553194A1 (en) * | 1983-10-05 | 1985-04-12 | Eternit Financiere | Method for fixing flat material samples and device for implementing it |
| CN1412504A (en) * | 2001-10-08 | 2003-04-23 | 中国科学院生态环境研究中心 | Semiconductor refrigerated low-temp. chromatograph enrichment and separation equipment |
| US20070060872A1 (en) * | 2005-02-14 | 2007-03-15 | Hall W D | Apparatus and methods for analyzing body fluid samples |
| CN101292148A (en) * | 2005-08-19 | 2008-10-22 | 加拿大血液中心 | Sample holder for dynamic light scattering |
| JP2010287729A (en) * | 2009-06-11 | 2010-12-24 | Okano Electric Wire Co Ltd | Heating/cooling test apparatus |
| CN202057637U (en) * | 2011-04-06 | 2011-11-30 | 江苏奥迪康医学科技有限公司 | Thermostatic structure of chromatographic column of glycolated hemoglobin analyzer |
| CN202133580U (en) * | 2011-06-28 | 2012-02-01 | 上海惠中医疗科技有限公司 | Heating mechanism for fully automatic glycosylated hemoglobin analyzer |
| CN202599917U (en) * | 2012-04-25 | 2012-12-12 | 江苏奥迪康医学科技有限公司 | Heating device in saccharification haemoglobin analyser clinical sample machine |
Non-Patent Citations (2)
| Title |
|---|
| 谢洁红 等: "两种糖化血红蛋白测定方法的比较", 《第三届全国临床检验实验室管理学术会议论文汇编》, 30 June 2005 (2005-06-30), pages 126 - 127 * |
| 郑华 等: "Bio-Rad VariantII 糖化血红蛋白分析仪改良试剂应用评价", 《军医进修学院学报》, vol. 32, no. 3, 31 March 2011 (2011-03-31), pages 259 - 262 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106996885A (en) * | 2017-03-24 | 2017-08-01 | 江苏奥迪康医学科技股份有限公司 | Glycolated hemoglobin analysis melts blood pool and its pre-temperature method |
| CN109406693A (en) * | 2018-12-09 | 2019-03-01 | 大连技嘉科技有限公司 | Glycolated hemoglobin analysis column oven |
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| Publication number | Publication date |
|---|---|
| CN102645494B (en) | 2013-06-26 |
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Address after: 212009 Zhenjiang city Jiangsu province area Dingmao latitude four Road No. 36 high tech Industrial Park, No. 4 Building 4 layer Patentee after: JIANGSU AUDICOM MEDICAL TECHNOLOGY Co.,Ltd. Address before: 212009 Zhenjiang city Jiangsu province area Dingmao latitude four Road No. 36 high tech Industrial Park, No. 4 Building 4 layer Patentee before: JIANGSU AUDICOM MEDICAL TECHNOLOGY Co.,Ltd. |
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Granted publication date: 20130626 |
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