CN102642931B - Synthesis of biological agent rich in phosphorous content in natural water resources and application thereof - Google Patents
Synthesis of biological agent rich in phosphorous content in natural water resources and application thereof Download PDFInfo
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- CN102642931B CN102642931B CN 201210095589 CN201210095589A CN102642931B CN 102642931 B CN102642931 B CN 102642931B CN 201210095589 CN201210095589 CN 201210095589 CN 201210095589 A CN201210095589 A CN 201210095589A CN 102642931 B CN102642931 B CN 102642931B
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Abstract
The invention discloses a synthesis of a biological agent rich in phosphorous content in natural water resources and an application thereof, by the adoption of raw materials of flavobacterium aquatile, potassium chloride, sodium hydrogen carbonate, culture medium and arundina chinensis in the method, and bacterial strain cells i.e. quasi yellow rod globulins are obtained by culturing and screening flavobacterium aquatile. 50-60m<3> water with phosphorous concentration of 0.1mg/L-0.2mg/L is placed in a culturing pool, arundina chinensis is moved into the pool, the growing area of arundina chinensis takes up the area of the culturing pool of 30-40 percent, the bacterial strain is dissolved with potassium chloride and sodium hydrogen carbonate according to certain proportion, and the corresponding volume ratio is 1:15:(25-30), the dissolvent is put into the culturing pool after being evenly mixed up, the dosing period is nine days, once every day, a material with strong combination with phosphorous of low concentration is produced by the flavobacterium aquatile in water, and 30 days later, concentrated factors rich in phosphorous of flavobacterium aquatile reach 61600-97500.
Description
Technical field
The present invention relates to the biotechnological formulation synthetic method of phosphorus content in a kind of enrichment natural water, belong to the biosynthesis technology field.
Background technology
Phosphorus in the natural water mainly is to exist with various phosphatic forms, and content is not high, and high effective phosphorus content is between 1.5 ~ 3.5umol/L.Phosphorus is one of essential element of biological growth, but because the natural water water yield is large, and do not flow, when phosphorus content in the water body during greater than 0.01 ~ 0.02mg/L, can cause that algae and other planktonic organisms breed rapidly in the water, be easy to eutrophication, the Dissolved Oxygen in Water amount descends, and the current transparency reduces, water quality deterioration, fish and other biological mortality, make water resources lose the utility value of the aspects such as cultivation and visit; Because containing nitrate and nitrite in the eutrophic water, people's long-term drinking also can be poisoned pathogenic.
At present, the treatment technology of the natural water eutrophication that generally adopts both at home and abroad mainly contains three classes: (1) chemical method: kill algae as adding copper sulfate, add the precipitation of molysite promotion phosphorus etc., but processing costs is high and easily cause secondary pollution; (2) physical method: as dredge bed mud, mechanical separation is crossed filtering algae, diversion erosion and deposition etc., and is simple to operate, non-secondary pollution, but often degree for the treatment of is low, cures the symptoms, not the disease; (3) biological-ecological restorative procedure: such as the microbial film take microorganism as the processing core, active sludge treatment technology, biomanipulation, biological floating bed technology, Treatment Technology of Constructed Wetland etc., simple to operate, treatment effect good, repair time is shorter, cost is low, do not produce secondary pollution.
In fact, extensively there is occurring in nature in biomagnification phenomenon.Microorganism in the mucus of rainbow trout surface has very strong methylation, can be converted into methyl mercury to inorganic mercury, and its enrichment to mercury reaches hundreds of thousands of doubly; Herba Eichhorniae can reach 120000 to the enrichment enrichment factor of chromium; Herba pteridis vittatae can reach several thousand times to the arsenic enrichment factor in the surrounding environment; Sea-tangle can reach several thousand, several ten thousand times to iodine biomagnification in the surrounding environment.But also do not have at present a kind of very high biology of bioconcentration factor that can enriched phosphorus, therefore, we are necessary to study a kind of biotechnological formulation, the phosphorus in the enrichment natural water effectively, thus effectively remove phosphorus in the water.
Summary of the invention
The present invention is directed to the occurring in nature biology not high to Phosphorus enrichment enrichment factor in the natural water, a kind of synthetic method of biotechnological formulation is provided, should intend yellow bar sphaeroprotein biological agent renders in the water body, so that the Purpleback Murdannia in the water body produces a kind of material that can be strong with the low phosphorus binding ability, cause Purpleback Murdannia that phosphorus is had strong inrichment, enrichment factor can reach several thousand or several ten thousand, thereby the phosphorus in the ambient water environment is got rid of, and solves the body eutrophication problem.
Raw material of the present invention is: flavobacterium aquatile, Repone K, sodium bicarbonate, substratum, Purpleback Murdannia, by cultivation, the rear acquisition of the screening strain cell to flavobacterium aquatile, this strain cell uses with the certain mass ratio with Repone K, sodium bicarbonate, synthetic this microbial preparation is rendered in the water body, so that the Purpleback Murdannia in the water body produces a kind of material that can be strong with the low phosphorus binding ability, cause Purpleback Murdannia that the bioconcentration of phosphorus is reached several thousand even several ten thousand, thereby farthest remove low phosphorus in the water.
The technical solution used in the present invention is:
The biotechnological formulation synthetic method of phosphorus content in a kind of enrichment natural water, it is raw material that the method adopts flavobacterium aquatile, Repone K, sodium bicarbonate, substratum, Purpleback Murdannia, by cultivation, the rear acquisition of the screening strain cell to flavobacterium aquatile, this strain cell than using, synthesizes this microbial preparation with certain mass with Repone K, sodium bicarbonate.
The building-up process of the biotechnological formulation of unit cell sphaeroprotein among the present invention:
(1) preparation of substratum
In mass, glucose 5 ~ 8g, dipotassium hydrogen phosphate 0.2 ~ 0.5g, sal epsom 0.1 ~ 0.2g, agar 10 ~ 12g,
Nitrocalcite 0.6 ~ 0.8g, sodium bicarbonate 0.5 ~ 0.8g, under pH=7.0 ~ 7.2 conditions with each components dissolved, stirring, adding distil water is settled to 1000mL, regulates pH=7.0 ~ 7.2, then packing, High Temperature Sterilization, for subsequent use;
(2) cultivation of plant sample
Cultivate a collection of Purpleback Murdannia in free of contamination pond, afterwards sterilization in 7 ~ 10 days moves in the glass aquarium again, keeps certain illumination, and temperature makes its plant healthy and strong, as the test plant sample;
(3) flavobacterium aquatile inoculation
Get 20 ~ 25mL substratum, under aseptic condition, adopt the inclined plane inoculating method, after inoculation is finished, cultivate mouth of pipe cotton beyond the Great Wall, place in the bio-incubator and cultivate, temperature is controlled at 35 ~ 37 ° of C, and incubation time is 25 ~ 35h;
(4) strain cell obtains
Bacterial classification in the incubator is taken out, add 3 ~ 5mL 0.1mol/L NaCl solution in culture tube, fully shake up, centrifugal 20 ~ 25min under supercentrifuge, rotating speed are 300 ~ 400r/min, and temperature is 10 ~ 15 ° of C, the elimination supernatant liquid; Under the similarity condition, centrifugal 20 ~ 25min extracts lower floor's milky white precipitate in the specific volume pipe, is flavobacterium aquatile strain cell, preserves under 2 ~ 6 ° of C of low temperature.
The using method of the biotechnological formulation of phosphorus content in a kind of enrichment natural water:
Get and contain water body 50 ~ 60m that phosphorus concentration is 0.01mg/L ~ 0.02mg/L
3Place cultivation pool, and immigration Purpleback Murdannia, its area accounts for 30% ~ 40% of cultivation pool area, again this bacterial strain and Repone K, sodium bicarbonate are dissolved by a certain percentage, its corresponding volume ratio 1:15:25 ~ 30, wherein, the concentration of bacterial strain is 0.01g/L ~ 0.05g/L, potassium chloride concentration is 0.1mol/L ~ 0.2mol/L, sodium bicarbonate concentration is 0.2mol/L ~ 0.3mol/L, renders in the cultivation pool after it is mixed, and the administration phase is the Ninth Heaven, throw in every day once, detected afterwards the phosphorus content in Purpleback Murdannia and the water body in 30 ~ 40 days.
The biotechnological formulation of the plan Flavobacterium sphaeroprotein that the present invention prepares is characterized in that outward appearance is the milky white precipitate thing, directly administration, solubleness is 99.99%, the transformation period is 7 days, the administration cycle is 3 days, and consumption is 0.01g/L ~ 0.05g/L, needs to preserve under 2 ~ 6 ° of C of low temperature.
The yellow bar sphaeroprotein of the plan that the present invention prepares biotechnological formulation, it is rendered in the water body, make the Purpleback Murdannia in the water body produce a kind of material that can be strong with the low phosphorus binding ability, cause Purpleback Murdannia that the Phosphorus enrichment enrichment factor is reached 61600 ~ 97500, phosphorus concentration in the ambient water environment has solved the body eutrophication problem less than 1ppb.
The invention has the beneficial effects as follows:
1. adopt biotechnology fully, technique is simple, can not bring secondary pollution to environment;
2. directly administration, the transformation period is 7 days, and the administration cycle is 9 days, and consumption is 0.01g/L ~ 0.05g/L;
3. the enrichment factor of Purpleback Murdannia enriched phosphorus reaches 61600 ~ 97500, and phosphorus content drops to concentration less than 1ppb from 0.01 ~ 0.02mg/L in the water body.
Embodiment
Raw material of the present invention is: adopting flavobacterium aquatile, Repone K, sodium bicarbonate, substratum, Purpleback Murdannia is raw material, by cultivation, the rear acquisition of the screening strain cell to flavobacterium aquatile, this strain cell uses with the certain mass ratio with Repone K, sodium bicarbonate, synthetic this microbial preparation is rendered in the water body, cause Purpleback Murdannia that the Phosphorus enrichment enrichment factor is reached 61600 ~ 97500, the phosphorus concentration in the ambient water environment is less than 1ppb.
(1) preparation of substratum
In mass, glucose 5 ~ 8g, dipotassium hydrogen phosphate 0.2 ~ 0.5g, sal epsom 0.1 ~ 0.2g, agar 10 ~ 12g,
Nitrocalcite 0.6 ~ 0.8g, sodium bicarbonate 0.5 ~ 0.8g, under pH=7.0 ~ 7.2 conditions with each components dissolved, stirring, adding distil water is settled to 1000mL, regulates pH=7.0 ~ 7.2, then packing, High Temperature Sterilization, for subsequent use;
(2) cultivation of plant sample
Cultivate a collection of Purpleback Murdannia in free of contamination pond, afterwards sterilization in 7 ~ 10 days moves in the glass aquarium again, keeps certain illumination, and temperature makes its plant healthy and strong, as the test plant sample;
(3) flavobacterium aquatile inoculation
Get 20 ~ 25mL substratum, under aseptic condition, adopt the inclined plane inoculating method, after inoculation is finished, cultivate mouth of pipe cotton beyond the Great Wall, place in the bio-incubator and cultivate, temperature is controlled at 35 ~ 37 ° of C, and incubation time is 25 ~ 35h;
(4) strain cell obtains
Bacterial classification in the incubator is taken out, add 3 ~ 5mL 0.1mol/L NaCl solution in culture tube, fully shake up, centrifugal 20 ~ 25min under supercentrifuge, rotating speed are 300 ~ 400r/min, and temperature is 10 ~ 15 ° of C, the elimination supernatant liquid; Under the similarity condition, centrifugal 20 ~ 25min extracts lower floor's milky white precipitate in the specific volume pipe, is flavobacterium aquatile strain cell, preserves under 2 ~ 6 ° of C of low temperature.
The using method of the biotechnological formulation of phosphorus content in a kind of enrichment natural water: get and contain water body 50 ~ 60m that phosphorus concentration is 0.01mg/L ~ 0.02mg/L
3Place cultivation pool, and immigration Purpleback Murdannia, its area accounts for 30% ~ 40% of cultivation pool area, again this bacterial strain and Repone K, sodium bicarbonate are dissolved by a certain percentage, its corresponding volume ratio 1:15:25 ~ 30, wherein, the concentration of bacterial strain is 0.01g/L ~ 0.05g/L, potassium chloride concentration is 0.1mol/L ~ 0.2mol/L, sodium bicarbonate concentration is 0.2mol/L ~ 0.3mol/L, renders in the cultivation pool after it is mixed, and the administration phase is the Ninth Heaven, throw in every day once, detected afterwards the phosphorus content in Purpleback Murdannia and the water body in 30 ~ 40 days.
Example 1
(1) preparation of substratum
In mass, get glucose 5g, dipotassium hydrogen phosphate 0.2g, sal epsom 0.1g, agar 10g, nitrocalcite 0.6g, sodium bicarbonate 0.5g, under the pH=7.0 condition with each components dissolved, stirring, distilled water is settled to 1000mL, regulates pH=7.0, packing, High Temperature Sterilization, for subsequent use;
(2) cultivation of plant sample
Cultivate a collection of Purpleback Murdannia in free of contamination pond, afterwards sterilization in 7 days moves in the glass aquarium again, keeps certain illumination, and temperature makes its plant healthy and strong, as the test plant sample;
(3) flavobacterium aquatile inoculation
Get the 20mL substratum, under aseptic condition, adopt the inclined plane inoculating method, after inoculation is finished, cultivate mouth of pipe cotton beyond the Great Wall, place in the bio-incubator and cultivate, temperature is controlled at 35 ° of C, and incubation time is 25h;
(4) strain cell obtains
Bacterial classification in the incubator is taken out, add 3mL 0.1mol/L NaCl solution in culture tube, fully shake up, centrifugal 20min under supercentrifuge, rotating speed are 300r/min, and temperature is 10 ° of C, the elimination supernatant liquid; Under the similarity condition, centrifugal 20min extracts lower floor's milky white precipitate in the specific volume pipe, is flavobacterium aquatile strain cell, preserves under 2 ° of C of low temperature.
The using method of the biotechnological formulation of phosphorus content in a kind of enrichment natural water: get and contain the water body 50m that phosphorus concentration is 0.01mg/L
3Place cultivation pool, and move into Purpleback Murdannia, its area accounts for 30% of cultivation pool area, again this bacterial strain and Repone K, sodium bicarbonate are dissolved by a certain percentage, its corresponding volume ratio 1:15:25, wherein, the concentration of bacterial strain is 0.01g/L, potassium chloride concentration is 0.1mol/L, sodium bicarbonate concentration is 0.2mol/L, renders in the cultivation pool after it is mixed, and the administration phase is the Ninth Heaven, throw in every day once, detected afterwards the phosphorus content in Purpleback Murdannia and the water body in 30 days.
The result shows: this biotechnological formulation enrichment factor to phosphorus in Purpleback Murdannia reaches 61600.
Example 2
(1) preparation of substratum
In mass, get glucose 7g, dipotassium hydrogen phosphate 0.3g, sal epsom 0.15g, agar 11g, nitrocalcite 0.7g, sodium bicarbonate 0.6g, with each components dissolved, stirring, distilled water is settled to 1000mL under the pH=7.1 condition, regulate pH=7.1, packing, High Temperature Sterilization, for subsequent use;
(2) cultivation of plant sample
Cultivate a collection of Purpleback Murdannia in free of contamination pond, afterwards sterilization in 8 days moves in the glass aquarium again, keeps certain illumination, and temperature makes its plant healthy and strong, as the test plant sample;
(3) flavobacterium aquatile inoculation
Get the 22mL substratum, under aseptic condition, adopt the inclined plane inoculating method, after inoculation is finished, cultivate mouth of pipe cotton beyond the Great Wall, place in the bio-incubator and cultivate, temperature is controlled at 36 ° of C, and incubation time is 30h;
(4) strain cell obtains
Bacterial classification in the incubator is taken out, add 4mL 0.1mol/L NaCl solution in culture tube, fully shake up, centrifugal 23min under supercentrifuge, rotating speed are 350r/min, and temperature is 12 ° of C, the elimination supernatant liquid; Under the similarity condition, centrifugal 22min extracts lower floor's milky white precipitate in the specific volume pipe, is flavobacterium aquatile strain cell, preserves under 4 ° of C of low temperature.
The using method of the biotechnological formulation of phosphorus content in a kind of enrichment natural water: get and contain the water body 55m that phosphorus concentration is 0.015mg/L
3Place cultivation pool, and move into Purpleback Murdannia, its area accounts for 35% of cultivation pool area, again this bacterial strain and Repone K, sodium bicarbonate are dissolved by a certain percentage, its corresponding volume ratio 1:15:28, wherein, the concentration of bacterial strain is 0.03g/L, potassium chloride concentration is 0.15mol/L, sodium bicarbonate concentration is 0.25mol, renders in the cultivation pool after it is mixed, and the administration phase is the Ninth Heaven, throw in every day once, detected afterwards the phosphorus content in Purpleback Murdannia and the water body in 35 days.
The result shows: this biotechnological formulation enrichment factor to nitrogen in the snakeheaded fish body reaches 78900.
Example 3
(1) preparation of substratum
In mass, get glucose 8g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.2g, agar 12g, nitrocalcite 0.8g, sodium bicarbonate 0.8g, with each components dissolved, stirring, distilled water is settled to 1000mL under the pH=7.2 condition, regulate pH=7.2, packing, High Temperature Sterilization, for subsequent use;
(2) cultivation of plant sample
Cultivate a collection of Purpleback Murdannia in free of contamination pond, afterwards sterilization in 10 days moves in the glass aquarium again, keeps certain illumination, and temperature makes its plant healthy and strong, as the test plant sample;
(3) flavobacterium aquatile inoculation
Get the 25mL substratum, under aseptic condition, adopt the inclined plane inoculating method, after inoculation is finished, cultivate mouth of pipe cotton beyond the Great Wall, place in the bio-incubator and cultivate, temperature is controlled at 37 ° of C, and incubation time is 35h;
(4) strain cell obtains
Bacterial classification in the incubator is taken out, add 5mL 0.1mol/L NaCl solution in culture tube, fully shake up, centrifugal 25min under supercentrifuge, rotating speed are 400r/min, and temperature is 15 ° of C, the elimination supernatant liquid; Under the similarity condition, centrifugal 25min extracts lower floor's milky white precipitate in the specific volume pipe, is flavobacterium aquatile strain cell, preserves under 6 ° of C of low temperature.
The using method of the biotechnological formulation of phosphorus content in a kind of enrichment natural water: get and contain the water body 60m that phosphorus concentration is 0.02mg/L
3Place cultivation pool, and move into Purpleback Murdannia, its area accounts for 40% of cultivation pool area, again this bacterial strain and Repone K, sodium bicarbonate are dissolved by a certain percentage, its corresponding volume ratio 1:15:30, wherein, the concentration of bacterial strain is 0.05g/L, potassium chloride concentration is 0.2mol/L, sodium bicarbonate concentration is 0.3mol/L, renders in the cultivation pool after it is mixed, and the administration phase is the Ninth Heaven, throw in every day once, detected afterwards the phosphorus content in Purpleback Murdannia and the water body in 40 days.
The result shows: this biotechnological formulation enrichment factor to nitrogen in the snakeheaded fish body reaches 97500.
Claims (1)
1. the application of the biotechnological formulation of phosphorus content in the enrichment natural water is characterized in that:
(1) preparation of substratum
In mass, glucose 5 ~ 8g, dipotassium hydrogen phosphate 0.2 ~ 0.5g, sal epsom 0.1 ~ 0.2g, agar 10 ~ 12g, nitrocalcite 0.6 ~ 0.8g, sodium bicarbonate 0.5 ~ 0.8g, under pH=7.0 ~ 7.2 conditions with each components dissolved, stirring, adding distil water is settled to 1000mL, regulate pH=7.0 ~ 7.2, then packing, High Temperature Sterilization, for subsequent use;
(2) cultivation of plant sample
Cultivate a collection of Purpleback Murdannia in free of contamination pond, afterwards sterilization in 7 ~ 10 days moves in the glass aquarium again, keeps certain illumination, and temperature makes its plant healthy and strong, as the test plant sample;
(3) flavobacterium aquatile inoculation
Get 20 ~ 25mL substratum, under aseptic condition, adopt the inclined plane inoculating method, after inoculation is finished, cultivate mouth of pipe cotton beyond the Great Wall, place in the bio-incubator and cultivate, temperature is controlled at 35 ~ 37 ° of C, and incubation time is 25 ~ 35h;
(4) strain cell obtains
Bacterial classification in the incubator is taken out, add 3 ~ 5mL 0.1mol/L NaCl solution in culture tube, fully shake up, centrifugal 20 ~ 25min under supercentrifuge, rotating speed are 300 ~ 400r/min, and temperature is 10 ~ 15 ° of C, the elimination supernatant liquid; Under the similarity condition, centrifugal 20 ~ 25min extracts lower floor's milky white precipitate in the specific volume pipe, is flavobacterium aquatile strain cell, preserves under 2 ~ 6 ° of C of low temperature;
(5) get and contain water body 50 ~ 60m that phosphorus concentration is 0.1mg/L ~ 0.2mg/L
3Place cultivation pool, and immigration Purpleback Murdannia, its area accounts for 30% ~ 40% of cultivation pool area, again with this flavobacterium aquatile strain cell and Repone K, sodium bicarbonate dissolves by a certain percentage, its corresponding volume ratio 1:15:25 ~ 30, wherein, the concentration of flavobacterium aquatile strain cell is 0.01g/L ~ 0.05g/L, potassium chloride concentration is 0.1mol/L ~ 0.2mol/L, and sodium bicarbonate concentration is 0.2mol/L ~ 0.3mol/L, renders in the cultivation pool after it is mixed, the administration phase is the Ninth Heaven, throw in once every day, and after 30 days, Purpleback Murdannia reaches 61600 ~ 97500 to the Phosphorus enrichment enrichment factor.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603251A (en) * | 2004-10-10 | 2005-04-06 | 武汉大学 | Method for removing nutrient salt in water |
| CN101050423A (en) * | 2007-03-19 | 2007-10-10 | 上海真美科技发展有限公司 | Composite preparation microbiological, and preparation method |
| CN102010065A (en) * | 2010-10-08 | 2011-04-13 | 天津水工业工程设备有限公司 | Comprehensive treatment method of landscape water eutrophication and algae overgrowth |
| CN102070237A (en) * | 2010-11-26 | 2011-05-25 | 常州大学 | COD degradation agent for removing sulfamide from industrial wastewater |
| CN102154142A (en) * | 2010-10-20 | 2011-08-17 | 湖南大学 | Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603251A (en) * | 2004-10-10 | 2005-04-06 | 武汉大学 | Method for removing nutrient salt in water |
| CN101050423A (en) * | 2007-03-19 | 2007-10-10 | 上海真美科技发展有限公司 | Composite preparation microbiological, and preparation method |
| CN102010065A (en) * | 2010-10-08 | 2011-04-13 | 天津水工业工程设备有限公司 | Comprehensive treatment method of landscape water eutrophication and algae overgrowth |
| CN102154142A (en) * | 2010-10-20 | 2011-08-17 | 湖南大学 | Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution |
| CN102070237A (en) * | 2010-11-26 | 2011-05-25 | 常州大学 | COD degradation agent for removing sulfamide from industrial wastewater |
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