CN102641497A - Medicinal composition for treating gastrointestinal diseases - Google Patents
Medicinal composition for treating gastrointestinal diseases Download PDFInfo
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- CN102641497A CN102641497A CN2012101543379A CN201210154337A CN102641497A CN 102641497 A CN102641497 A CN 102641497A CN 2012101543379 A CN2012101543379 A CN 2012101543379A CN 201210154337 A CN201210154337 A CN 201210154337A CN 102641497 A CN102641497 A CN 102641497A
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- hericium erinaceus
- gastroenteropathy
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Abstract
The invention relates to a medicinal composition for treating gastrointestinal diseases, which contains the activity of cellulase. The composition adopts a secondary hedgehogt fungus culture prepared by a new process, namely the secondary hedgehogt fungus culture obtained by adopting aseptic culture, secondary culture, low-temperature drying and low-temperature sterilization, and then the secondary hedgehogt fungus culture is further combined with five traditional Chinese medicines for preparation. As the composition adopts the hedgehogt fungus culture prepared by the new process, the activity of the cellulase is retained, and the medicinal effects are improved.
Description
Technical field
The present invention relates to a kind of gastroenteropathy and use drug composition.
Background technology
HOUTOU JIANWEI LING is the NF kind, and its function cures mainly and is: soothing liver-QI stomach function regulating, regulating QI to relieve pain.Be used for incoordination between the liver and stomach, gastral cavilty distending pain over the hypochondrium, vomiting acid regurgitation; Chronic gastritis, gastric and duodenal ulcers belong to above-mentioned patient; It is a kind of specific drug of treating gastropathy.Particularly helicobacter pylori being had stronger inhibitory or killing effect, take for a long time that canceration has certain preventive effect to digestive tract, and can alleviate its symptom, have no side effect, is the special medicine of treating gastropathy in the market.
The HOUTOU JIANWEI JIAONANG quality standard recorded into " one one of Chinese pharmacopoeia version in 2010, the HOUTOU JIANWEI JIAONANG of stipulating on pharmacopeia prescription is: Hericium erinaceus culture 160g, Endoconcha Sepiae 80g, vinegar Rhizoma Corydalis 40g, Radix Paeoniae Alba 40g, Rhizoma Cyperi (processed with vinegar) 40g, Radix Glycyrrhizae 40g, production technology is: above Six-element; Get Hericium erinaceus culture 80g, decocte with water secondary, each 2 hours; Filter, merging filtrate is evaporated in right amount, adds remaining Hericium erinaceus culture; Mixing, drying is ground into fine powder; Ground spices such as all the other Endoconcha Sepiaes are broken into fine powder, sieve, and with last fine powder and an amount of starch mixing, sterilization incapsulates and processes 1000, promptly gets.
HOUTOU JIANWEI LING good effect, popularity are high, reputation is good, market potential is big, is a very distinctive product, is the product of biotechnology medicine and traditional medicine perfect adaptation, and the primary raw material Hericium erinaceus culture has been brought into play important drug activity.
Hericium erinaceus culture property is flat; Sweet in the mouth; The sharp the five internal organs of ability are aid digestion, have curative effects such as antiulcer, anti-inflammatory, antitumor, defying age, hepatoprotective, blood sugar lowering, blood fat reducing and blood pressure lowering, and have effects such as suppressing and killing helicobacter pylori, staphylococcus aureus, escherichia coli; Medical value is high, and market uses potentiality big.
Hericium erinaceus culture rich cellulose enzyme, and cellulase acts on digestive tract, when decomposition of cellulose, plays biocatalysis; Can improve digestive tract environment, increase acidity, activate pepsin; Increase gastrointestinal enzyme secretory volume and activity, reduce alimentary canal content viscosity, help gastrointestinal tract digesting and assimilating food; But other Chinese medicines of compatibility are used for symptoms such as abdominal distention, indigestion, inappetence, and the power of its vigor is directly connected to the curative effect of product.As a kind of compound protein, its stability and active performance receive the influence of several factors, how to keep the sterilization front and back active constant, become critical core technology.
But HOUTOU JIANWEI LING is according to existing production technology; It is best that its curative effect does not reach, and the cellulase that the bioactive substance of Hericium erinaceus culture is particularly protein-based is inactivated through moist heat sterilization, heat; Thereby also need further improve, to reach optimum efficiency.Through detecting, carboxymethylcelluloenzyme enzyme activity is 133U/g in the Hericium erinaceus culture, and carboxymethylcelluloenzyme enzyme activity is 183U/g in the secondary Hericium erinaceus culture, and HOUTOU JIANWEI LING is produced the back cellulase activity by original production process and is almost 0.
Summary of the invention
In order to overcome the problem of the cellulase inactivation that exists in the prior art, the present invention provides a kind of new gastroenteropathy to use drug composition, makes the cellulase activity in the medicine preserve to greatest extent.Pharmaceutical composition of the present invention, carboxymethylcelluloenzyme enzyme activity are 20 ~ 40U/g, have improved the curative effect of medicine effectively.
The present invention realizes according to following technical scheme:
A kind of gastroenteropathy is used drug composition, and the acid carboxymethylcelluloenzyme enzyme activity of wherein per 1 gram finished product is not less than 20U.
Above-mentioned gastroenteropathy is used drug composition, wherein comprises secondary Hericium erinaceus culture, Endoconcha Sepiae, Radix Paeoniae Alba, vinegar Rhizoma Corydalis, Rhizoma Cyperi (processed with vinegar), Radix Glycyrrhizae, and described secondary Hericium erinaceus culture adopts following method preparation:
(1) aseptic culture: Hericium erinaceus culture is through medium sterilization, and inoculation in 100,000 grades of clean areas, aseptic condition were cultivated 40-50 days down, and temperature is 20 ℃-30 ℃, prevents to pollute;
(2) second incubation: promptly get Hericium erinaceus culture after once bottle is drawn in cultivation, change in the plastic bag again and cultivated 5-10 days in culturing room, cultivation temperature is 20-30 ℃, takes out;
(3) cold drying: do not surpass under 60 ℃ the condition dry in temperature;
(4) low temperature sterilization: sterilize above under 60 ℃ the condition in temperature.
Above-mentioned gastroenteropathy is used drug composition, contains acid carboxymethylcelluloenzyme enzyme activity in the wherein said secondary Hericium erinaceus culture and is not less than 150U/g.
Above-mentioned gastroenteropathy is used drug composition, and the step (3) of wherein said secondary Hericium erinaceus culture method for preparing adopts microwave drying technology or vacuum and low temperature continuous drying technology.
Above-mentioned gastroenteropathy is used drug composition, and the step (4) of wherein said secondary Hericium erinaceus culture method for preparing adopts a kind of in Co60 radiation sterilization, the ultra-vioket radiation sterilization.
Above-mentioned gastroenteropathy is used drug composition, and wherein the mass ratio of secondary Hericium erinaceus culture, Endoconcha Sepiae, Radix Paeoniae Alba, vinegar Rhizoma Corydalis, Rhizoma Cyperi (processed with vinegar), Radix Glycyrrhizae is 4: 2: 1: 1: 1.
Above-mentioned gastroenteropathy is used drug composition, and wherein Endoconcha Sepiae, Radix Paeoniae Alba, vinegar Rhizoma Corydalis, Rhizoma Cyperi (processed with vinegar), Radix Glycyrrhizae all pass through sterilization treatment.
Adopt product of the present invention, kept cellulase activity, improved drug effect.Specific as follows:
1, cellulase activity is high in the Chinese medicine composition of the present invention, is applicable to gastroenteropathy, and digestion promoting is made good use of, and obviously is different from other Chinese patent medicine products.Cellulase acts on digestive tract, can improve digestive tract environment, increases acidity; Activate pepsin, increase gastrointestinal enzyme secretory volume and activity, reduce alimentary canal content viscosity; Help gastrointestinal tract to the digesting and assimilating of food, the power of its vigor is directly connected to the curative effect of product.
2, adopt vacuum and low temperature continuous drying technology, preserve active component.Hericium erinaceus culture contains bioactive ingredients and multiple volatile ingredients such as enzyme, protein, belongs to the heat sensitivity medical material, adds the thermal lability inactivation; And it concentrates the extractum viscosity height that makes through extracting, and is difficult for drying, and is active constant before and after how keeping dry, sterilizing; Become critical technical barrier, adopt fully automatic vacuum low temperature continuous drying technology, both can keep effective active component substantially; The best curative effect of performance medical material makes Hericium erinaceus culture and extractum intensive drying thereof again, and appearance luster is good; Fully enclosed automatic has certain bactericidal action simultaneously.
3, the present invention adopts the secondary solid fermentation culture medium, more helps mycelium and changes into effective ingredient, has produced the change of matter like this, and drug effect is better, plans the secondary Hericium erinaceus culture and accurately is expressed as the Hericium erinaceus (Bull. Ex Fr.) Pers. mycoplasma.After one time solid fermentation was cultivated, because of local anoxia in the bottle, particularly the bottle bottom was divided, and mycelia is failed the thorough decomposition and inversion of culture medium, and color is darker, thoroughly transforms culture medium in order to make mycelium, carries out second incubation.After once bottle is drawn in cultivation, change in the plastic bag again and cultivate certain hour in culturing room, take out, oven dry promptly gets.Through Hericium erinaceus culture and secondary Hericium erinaceus culture are detected comparison, the result is following:
The carboxymethylcelluloenzyme enzyme activity of Hericium erinaceus culture and secondary Hericium erinaceus culture relatively
4, the aseptic butt joint of medicated powder mixes the raising carboxymethylcelluloenzyme enzyme activity, heightens the effect of a treatment.The former technology of HOUTOU JIANWEI LING adopts 121 ℃ of steam sterilizations to handle before the preparation final molding 30 minutes mixed powder; So that microbial limit reaches the NF regulation; But high temperature long-time operation; Multiple effective substances such as volatile ingredient, enzyme and protein in the medicinal mycoplasma of this special biology of monarch drug Hericium erinaceus culture are destroyed, and especially acid carboxymethylcelluloenzyme enzyme activity has had a strong impact on the curative effect performance of HOUTOU JIANWEI LING.For this reason, adopt directly carrying out the aseptic mixing of docking with the Hericium erinaceus culture of aseptic culture acquisition after other medicated powder sterilization treatment such as Endoconcha Sepiae, the Radix Paeoniae Alba.Measure through check, the result show the aseptic butt joint HOUTOU JIANWEI LING of medicated powder not only microbial limit meet the NF relevant regulations, and acid carboxymethylcelluloenzyme enzyme activity improves greatly.
Former technology HOUTOU JIANWEI LING docks the acid carboxymethylcelluloenzyme enzyme activity of HOUTOU JIANWEI LING relatively with aseptic
Acid carboxymethylcelluloenzyme enzyme activity detection method is following:
The preparation of reference substance solution: the anhydrous glucose reference substance that is dried to constant weight of learning from else's experience 105 ℃ is an amount of, accurately claims surely, adds water and processes the solution that every 1ml contains 1mg, promptly gets.
The preparation of standard curve: precision is measured reference substance solution 2.0ml, 4.0ml, 6.0ml, 8.0ml, 10.0ml, puts respectively in the 10ml volumetric flask, respectively adds water to scale, shakes up.Precision is measured above-mentioned solution 1.5ml respectively, puts in the 25ml tool plug scale test tube, and each precision adds pH4.8 citrate buffer 0.5ml, mixing; The accurate immediately DNS reagent 1.5ml that adds shakes up, and puts in the boiling water bath and is incubated 15 minutes, takes out; The tap water cooling is 10 minutes rapidly, adds water to scale again, shakes up, and leaves standstill 1.5 hours; With the reagent corresponding is blank, the photograph ultraviolet visible spectrophotometry (" an appendix V of Chinese pharmacopoeia version in 2010 A), measure absorbance in the 540nm wavelength; With the absorbance is vertical coordinate, and glucose amount is an abscissa, the drawing standard curve.
The preparation of need testing solution: get the about 2.0g of Hericium erinaceus culture or secondary Hericium erinaceus culture fine powder (crossing sieve No. two), the accurate title, decide, and puts in the tool plug conical flask, and precision adds entry 200ml; Close plug is claimed to decide weight, and jolting 3 hours claims to decide weight again; Water is supplied the weight that subtracts mistake, shakes up, and promptly gets.This liquid should face uses new system.
Algoscopy: precision is measured 1% carboxymethylcellulose sodium solution 1.5ml and is put in the 25ml tool plug scale test tube, and precision adds need testing solution 0.5ml, mixing; Put in 50 ℃ of water-baths and be incubated 30 minutes; Take out, the sighting target directrix curve prepare the method item under, from " DNS of precision adding immediately reagent 1.5ml "; Measure absorbance, the weight (mg) of reading reducing sugar the need testing solution from standard curve in accordance with the law.Blank pipe adds 1% carboxymethylcellulose sodium solution 1.5ml, puts in 50 ℃ of water-baths insulation 30 minutes, takes out, and precision adds need testing solution 0.5ml, mixing, the sighting target directrix curve prepare the method item under, from " DNS of precision adding immediately reagent 1.5ml ", carry out in accordance with the law.Be calculated as follows:
U=A×1/0.5×n×2
U in the formula---every 1g contains acid carboxymethylcelluloenzyme enzyme activity unit, U/g;
The reducing sugar weight that A---absorbance checks on standard curve, mg;
N---diluted sample multiple;
2---time scale factor.
1g solid enzyme under 50 ± 1 ℃, pH4.8 condition, hydrolysis in 1 hour 1% sodium carboxymethyl cellulose substrate, producing the reducing sugar amount that is equivalent to the 1mg glucose is an acid carboxymethylcelluloenzyme enzyme activity unit, representes with U/g.
The acid carboxymethylcelluloenzyme enzyme activity of every 1g should be not less than 150U in the secondary Hericium erinaceus culture, and the acid carboxymethylcelluloenzyme enzyme activity of every 1g should be not less than 20U in the HOUTOU JIANWEI LING.
Description of drawings
1, Fig. 1 be gastroenteropathy of the present invention with enzymatic reaction temperature in the drug composition to the influence of enzyme activity determination.
The specific embodiment
Embodiment 1
1, aseptic culture: culture medium is through sterilization, and inoculation in 100,000 grades of clean areas, aseptic condition were cultivated 45 days down, and temperature is 25 ℃, prevents to pollute;
2, second incubation: promptly get Hericium erinaceus culture after once bottle is drawn in cultivation, change in the plastic bag again and cultivated 7 days in culturing room, cultivation temperature is 25 ℃, takes out;
3, cold drying: be under 45 ℃ the condition, to adopt vacuum and low temperature continuous drying technology dry in temperature;
4, low temperature sterilization: be to take the mode of ultraviolet sterilization to sterilize under 45 ℃ the condition in temperature;
5, preparation clear paste: get secondary Hericium erinaceus culture 80 grams of above-mentioned preparation, decocte with water filters, and filtrate decompression is condensed into the clear paste that relative density is 1.15 (70 ℃);
6, adding powder: clear paste and Endoconcha Sepiae powder 80 grams, Radix Paeoniae Alba powder 40 grams, vinegar Yanhusuo 40 grams, Rhizoma Cyperi (processed with vinegar) powder 40 grams, the Radix Glycyrrhizae powder 40 of above-mentioned preparation are restrained mix homogeneously; Drying is ground into fine powder, sieves; Sterilization; Add secondary Hericium erinaceus culture powder 80 grams, mixing incapsulates and processes 1000.
Claims (7)
1. a gastroenteropathy is used drug composition, it is characterized in that the acid carboxymethylcelluloenzyme enzyme activity of per 1 gram finished product is not less than 20U.
2. gastroenteropathy as claimed in claim 1 is used drug composition, it is characterized in that comprising secondary Hericium erinaceus culture, Endoconcha Sepiae, Radix Paeoniae Alba, vinegar Rhizoma Corydalis, Rhizoma Cyperi (processed with vinegar), Radix Glycyrrhizae, and described secondary Hericium erinaceus culture adopts following method preparation:
(1) aseptic culture: culture medium is through sterilization, and inoculation in 100,000 grades of clean areas, aseptic condition were cultivated 40-50 days down, and temperature is 20 ℃-30 ℃, prevents to pollute;
(2) second incubation: promptly get Hericium erinaceus culture after once bottle is drawn in cultivation, change in the plastic bag again and cultivated 5-10 days in culturing room, cultivation temperature is 20-30 ℃, takes out;
(3) cold drying: do not surpass under 60 ℃ the condition dry in temperature;
(4) low temperature sterilization: sterilize above under 60 ℃ the condition in temperature.
3. gastroenteropathy as claimed in claim 2 is used drug composition, it is characterized in that the acid carboxymethylcelluloenzyme enzyme activity of described secondary Hericium erinaceus culture is not less than 150U/g.
4. gastroenteropathy as claimed in claim 2 is used drug composition, it is characterized in that the step (3) of said secondary Hericium erinaceus culture method for preparing adopts microwave drying technology or vacuum and low temperature continuous drying technology.
5. gastroenteropathy as claimed in claim 2 is used drug composition, and the step (4) that it is characterized in that said secondary Hericium erinaceus culture method for preparing adopts a kind of in Co60 radiation sterilization, the ultra-vioket radiation sterilization.
6. gastroenteropathy as claimed in claim 2 is used drug composition, and the mass ratio that it is characterized in that secondary Hericium erinaceus culture, Endoconcha Sepiae, Radix Paeoniae Alba, vinegar Rhizoma Corydalis, Rhizoma Cyperi (processed with vinegar), Radix Glycyrrhizae is 4: 2: 1: 1: 1.
7. gastroenteropathy as claimed in claim 2 is used drug composition, it is characterized in that Endoconcha Sepiae, Radix Paeoniae Alba, vinegar Rhizoma Corydalis, Rhizoma Cyperi (processed with vinegar), Radix Glycyrrhizae all pass through sterilization treatment.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195052A (en) * | 2014-08-12 | 2014-12-10 | 湖南新汇制药股份有限公司 | Hericium erinaceus mycelium and culture method thereof |
| CN106509063A (en) * | 2016-11-07 | 2017-03-22 | 黑龙江省科学院技术物理研究所 | Method for restraining brown stain of hericium erinaceus through <60>Co-gamma radiation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1736454A (en) * | 2004-08-19 | 2006-02-22 | 湖南新汇制药有限公司 | Process for preparing concentration type stomach-invigorating medicine of hericium erinaceus |
| CN1883634A (en) * | 2005-06-22 | 2006-12-27 | 湖南新汇制药有限公司 | Preparation process of stomach-caring Chinese medicine with monkey head mushroom |
| CN101264202A (en) * | 2008-04-23 | 2008-09-17 | 湖南新汇制药有限公司 | Hericium erinaceus digestion-promoting Chinese medicinal composition and preparation thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1736454A (en) * | 2004-08-19 | 2006-02-22 | 湖南新汇制药有限公司 | Process for preparing concentration type stomach-invigorating medicine of hericium erinaceus |
| CN1883634A (en) * | 2005-06-22 | 2006-12-27 | 湖南新汇制药有限公司 | Preparation process of stomach-caring Chinese medicine with monkey head mushroom |
| CN101264202A (en) * | 2008-04-23 | 2008-09-17 | 湖南新汇制药有限公司 | Hericium erinaceus digestion-promoting Chinese medicinal composition and preparation thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195052A (en) * | 2014-08-12 | 2014-12-10 | 湖南新汇制药股份有限公司 | Hericium erinaceus mycelium and culture method thereof |
| CN106509063A (en) * | 2016-11-07 | 2017-03-22 | 黑龙江省科学院技术物理研究所 | Method for restraining brown stain of hericium erinaceus through <60>Co-gamma radiation |
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| CN102641497B (en) | 2015-01-21 |
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Effective date of registration: 20160612 Address after: 410200 Hunan Province, Wangcheng City Economic Development Zone Changsha only rockfield Road No. 18 Patentee after: Hunan Xinhui Pharmaceutical Co., Ltd. Address before: 410200 Hunan Province, Wangcheng City Economic Development Zone Changsha only rockfield Road No. 18 Patentee before: He Shujin |