CN102641349B - Traditional Chinese medicine composite for resisting ephedrine damage and production technology thereof - Google Patents
Traditional Chinese medicine composite for resisting ephedrine damage and production technology thereof Download PDFInfo
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- CN102641349B CN102641349B CN201210153013.3A CN201210153013A CN102641349B CN 102641349 B CN102641349 B CN 102641349B CN 201210153013 A CN201210153013 A CN 201210153013A CN 102641349 B CN102641349 B CN 102641349B
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Abstract
The invention discloses a traditional Chinese medicine composite for resisting ephedrine tissue damage, and the traditional Chinese medicine composite comprises the following components in proportion by weight: 40% to 50% of radix bupleuri, 40% to 50% of radices salviae miltiorrhizae and 5% to 15% of red dates. A preparation method of an aqueous solution of the traditional Chinese medicine composite comprises the following steps of: firstly, respectively adding water in the radix bupleuri, the radices salviae miltiorrhizae and the red dates with the equal quantity, wherein the quantity of the water is five times of that of each material; immersing the materials for 1 hours and decocting the materials for 30 minutes; filtering out liquid medicines for standby; and respectively adding water in dregs of the decoction, decocting the dregs for 30 minutes and filtering out liquid medicines for standby, wherein the quantity of the water is five times of that of each dreg; secondly, respectively combining each two liquid medicines in the first step and concentrating each two liquid medicines to constant volume of 250mL, thus obtaining concentrated solutions; and thirdly, uniformly mixing the concentrated solutions in the step two according to the formula proportion, thus obtaining the traditional Chinese medicine composite. The traditional Chinese medicine composite can be used for improving the oxidation resistance of tissue cells, inhibiting the lipid peroxidation of the cells, enhancing the viability of the cells, inhibiting the apoptosis of the cells, retarding the damage of the tissue cells, promoting the repair of the tissue cells and improving the constitution of patients, thus having a certain protection effect on the tissue cells and a certain treatment or rehabilitation effect on animals addicted to ephedrine.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate in particular to Chinese medicine against drug dependence compositions and the production technology thereof of a kind of anti-ephedrine tissue injury.
Background technology
Analeptic abuse is the serious social problem in the world today and medical problem; research Chinese herbal medicine is to the protective effect of animal body and the Withdrawal Effect to analeptic addiction animal; research and develop the new Chinese medicine preparation that has no side effect and there is Withdrawal Effect, all significant for the development of medical career and prohibiting drug and fighting drug, maintain social stability.
Ephedrine also claims ephedrine, it is central stimulants, can cause spiritual sense of euphoria, the excitement of generation property and hallucination, apply excessive or abuse and easily cause heart and central nervous system's toxic and side effects, produce the dependency to medicine, cause mental disorder and dystropy, ephedrine can cause histiocyte oxidative damage, when serious, can cause death.In addition, ice is analeptic methamphetamine (metamfetamine), synthetic through methylating by amfetamine, also can through deoxidation treatment, be obtained by ephedrine, make ephedrine ice making poison for malicious molecule, and then the drugs such as output head-shaking pill of regenerating, human or animal sucks methamphetamine hydrochloride (metamfetamine) can cause that disease is even dead, after Mus brain cell contacts with metamfetamine, can promote apoptosis, ephedrine can cause the decline of histiocyte polyphenoils enzyme activity and histiocyte damage.The organization protection or the anti-additive medicament that also there is no at present the anti-ephedrine damage of special efficacy.
Summary of the invention
The Chinese medicine composition that the object of this invention is to provide a kind of anti-ephedrine tissue injury, to reach anti-ephedrine damage, protective tissue cell, promotes histiocyte reparation, eliminates the psychological factor after ephedrine addiction and thoroughly gives up the object of drug addiction.
Two of object provides a kind of preparation method of this Chinese medicine composition water preparation.
The technical scheme adopting is:
A Chinese medicine composition for anti-ephedrine tissue injury, adopts following weight ratio formula:
Radix Bupleuri 40-50%, Radix Salviae Miltiorrhizae 40-50%, Fructus Jujubae 5-15%.
The formula optimization of the Chinese medicine composition of ，Kang ephedrine tissue injury further:
Radix Bupleuri 45%, Radix Salviae Miltiorrhizae 45%, Fructus Jujubae 10%.
A preparation method for the Chinese medicine composition water preparation of ephedrine tissue injury, this preparation method comprises the following steps:
(1) get respectively Radix Bupleuri, Radix Salviae Miltiorrhizae, the Fructus Jujubae of equivalent, respectively add 5 times of amounts of water, soak 1 hour, decoct 30 minutes, leach medicinal liquid standby, medicinal residues respectively add 5 times of amounts of water again, decoct 30 minutes, leach medicinal liquid standby;
(2) get step (1) twice medicinal liquid, after merging respectively, concentrate and be settled to 250 mL, make concentrated solution;
(3) get each concentrated solution of step (2), by described formula proportion, evenly mix.
The present invention mainly utilizes Radix Bupleuri analgesic, calm, analgesia, antiinflammatory, improve immunologic function, anti-liver injury, Antiradiation injury effect, Radix Salviae Miltiorrhizae improves cardiac function, improve microcirculation, promote tissue repair and regeneration and the effect protecting the liver, Fructus Jujubae benefiting qi and nourishing blood, tranquillizing and allaying excitement, enhancing human body immunity power, antiallergic, anticancer effect, science compatibility, improve histiocytic oxidation resistance, suppress cytolipin peroxidating, by improving cytoactive, inhibited apoptosis, slow down histiocyte damage, promote histiocyte reparation, improve patient's body constitution, histiocyte is had to certain protective role, treatment withdrawal symptom and elimination residual hazard, ephedrine addiction animal is had to certain treatment or Withdrawal Effect.
Especially for a long time suck the patient of ephedrine addiction, biological organs tissue damaged, health is weaker, physiological dependence and psychological dependence, cannot eliminate in a short time, the present invention can be used as and forces to give up, to slow down histiocyte damage, promote histiocyte reparation, effectively eliminate physiological dependence and psychological dependence and treat or give up ephedrine drug addiction.
In addition, junkie is through treatment after a while, drug abstainer just can be broken away from health to anti-depressant dependence, but some symptoms also may stay on a period of time, as withdrawal symptoms such as insomnia, agitation, anxiety, whole body pain, spirit depressings, these protracted symptoms also may reach the several months even for more time, be easy to cause detoxification person to relapse, the medicine of the anti-ephedrine of the present invention tissue injury, can be used as and maintain treatment, can effectively suppress to relapse, remove and give up rear protracted syndrome.
The present invention is not containing dependent drug and any anesthetis, also without any synthetic, belong to pure Chinese medicinal preparation, there is benefiting qi and nourishing blood, tranquillizing and allaying excitement, improve microcirculation, enhancing human body immunity power, promote tissue repair and regeneration and the effect protecting the liver, be applicable to inject the treatment that maintains after the detoxification treatment of the drug addiction persons such as ephedrine or sucking person and detoxification.
The present invention is in manufacture process, and technique is simple, pollution-free, small investment, and medicine is inexpensive, draws materials conveniently.There is curative effect high, have no side effect, the features such as taking convenience, suitable professional narcotic house, sanatorium, hospital and family are used.
The specific embodiment
Embodiment 1, and the Chinese medicine composition of a kind of anti-ephedrine tissue injury mainly comprises the component of following weight ratio: Radix Bupleuri 45%, Radix Salviae Miltiorrhizae 45%, Fructus Jujubae 10%.
Embodiment 2, and the Chinese medicine composition of a kind of anti-ephedrine tissue injury mainly comprises the component of following weight ratio: Radix Bupleuri 50%, Radix Salviae Miltiorrhizae 45%, Fructus Jujubae 5%.
Embodiment 3, and the Chinese medicine composition of a kind of anti-ephedrine tissue injury mainly comprises the component of following weight ratio: Radix Bupleuri 40%, Radix Salviae Miltiorrhizae 50%, Fructus Jujubae 10%.
Embodiment 4, and the Chinese medicine composition of a kind of anti-ephedrine tissue injury mainly comprises the component of following weight ratio: Radix Bupleuri 45%, Radix Salviae Miltiorrhizae 40%, Fructus Jujubae 15%.
The present invention can be made into water preparation or capsule.
A preparation method for the Chinese medicine composition water preparation of ephedrine tissue injury, this preparation method comprises the following steps:
(1) get respectively Radix Bupleuri, Radix Salviae Miltiorrhizae, the Fructus Jujubae of equivalent, respectively add 5 times of amounts of water, soak 1 hour, decoct 30 minutes, leach medicinal liquid standby, medicinal residues respectively add 5 times of amounts of water again, decoct 30 minutes, leach medicinal liquid standby;
(2) get step (1) twice medicinal liquid, after merging respectively, concentrate and be settled to 250 mL, make concentrated solution;
(3) get each concentrated solution of step (2), by described proportioning, mix.
The preparation method of capsule of the present invention: described Chinese medicine composition is dried or dried, sieve through mechanical activation comminution, use capsule splendid attire after evenly mixing by described proportioning.
zoopery of the present invention is as follows:
medicinal liquid preparation
Get respectively Radix Bupleuri, Radix Salviae Miltiorrhizae, each 500 g of Fructus Jujubae, respectively add 5 times of amounts of water, soak 1 h, decoct 30 min, leach respectively medicinal liquid standby, medicinal residues respectively add 5 times of amounts of water again, decoct 30 min, leach respectively medicinal liquid standby.After respectively twice medicinal liquid being merged, concentrate and be settled to 250 mL, make concentrated solution; Each concentrated solution is pressed Radix Bupleuri 45%, Radix Salviae Miltiorrhizae 45%, the even mixing for standby use of Fructus Jujubae 10% ratio.
model is set up and administration
Select 72 of the kunming mices (body weight 4 ~ 5 g, Lanzhou University's Experimental Animal Center provides) of raw 3-5 days, experiment prospective adaptation is raised one week, is then divided at random 3 groups (matched group, ephedrine group and Chinese drug-treated group, 24 every group).Ephedrine group and Chinese drug-treated group animal are first injected ephedrine 15 d continuously with ascending-dose abdominal cavity, set up ephedrine dependent Mice addiction model (at 1-5,6-10, it is 2.0 that 11-15 d injects respectively 0.2 mL concentration, the ephedrine solution of 3.0,4.0 mg/mL, every day (9:00am, 16:00pm) twice, matched group is injected isopyknic normal saline every day); After modeling, Chinese drug-treated group is given up (11-15 d is the Chinese medicine of gavage 0.2,0.3,0.4 mL respectively for 1-5 after modeling, 6-10, twice of every day), the normal saline of matched group and ephedrine group gavage equivalent with ascending-dose gavage Chinese medicine.
enzyme assay
After laboratory animal administration, respectively at giving up 5,10,15 d, through eye socket rear vein beard blood sampling 1-2 mL, sacrificed by decapitation, gets blood room temperature and places 1 h subsequently at once, 2500 r/ min centrifugal (10 min), and serum is prepared in separation.Meanwhile, cut rapidly hepatic tissue, rinsing in the normal saline of pre-cooling, filter paper blots, and after accurately weighing, drips the normal saline of 9 times of nephridial tissue weight, enter homogenate in frozen water in homogenizer (5 min), with 3000 r/min centrifugal (10 min), isolate supernatant.With micropipettor, draw serum or supernatant, according to the operation requirements (test kit is purchased from Nanjing and builds up Bioengineering Research Institute) of superoxide dismutase (SOD), catalase (CAT), malonaldehyde (MDA), glutamic oxaloacetic transaminase, GOT (GOT) and glutamate pyruvate transaminase (GPT) test kit, with UV-VIS spectrophotometer(U-1800 type Tokyo Japan) full-automatic spectrophotometer detection SOD, CAT, GOT, the content of GPT vigor and MDA.
histological observation
To Chinese medicine, giving up 5 respectively, 10,15 d get murine liver tissue several piece, clean with the normal saline flushing of pre-cooling, drop in 10% neutral formalin liquid and fix 24 h, routine paraffin wax embedding, serial section (6 μ m), H.E dyeing, under optical microscope (Olympus, FX-35WA, Japan), observe and take the photograph sheet.
immunohistochemistry
By hepatic tissue paraffin section de-waxing, to water, microwave treatment is carried out antigen retrieval, 3% H
2o
2hatch and eliminate intrinsic oversxidase activity, normal goats (Capra genus) serum incubated at room (30min) is to seal nonspecific reaction site, and primary antibodie is 1: 100 with the anti-Bax albumen of rabbit and c-Fos(working concentration), put 4 ℃ of refrigerator overnight incubation; Drip biotin labeled two anti-(goat anti-rabbit igg antiserums), incubated at room (30 min); Drip streptomycin avidin-peroxide multienzyme complex working solution, incubated at room (30 min); Blank replaces primary antibodie with PBS, DAB colour developing, haematoxylin redyeing, conventional dehydration, transparent, mounting, om observation take pictures (Bax albumen and c-Fos, SABC test kit and DAB developer are purchased from biological engineering company limited of Zhong Shan Golden Bridge).
surface density value is measured
By the hepatic tissue several piece fixing, serial section after directed embedding (6 μ m), each wax band pastes 1, and totally 10, sheet (* 400) is observed, is taken the photograph in 12 visuals field of every random selection of section.Adopt grid test macro, Point of intersection count method is calculated respectively the surface density value of apoptotic cell and positive expression cell.
statistical analysis
With SPSS 13.0 softwares, carry out statistical analysis processing, data are with representing, between each group, mean relatively adopts two tail t checks,
p<0.05 represents significant difference,
p<0.0l is that difference is extremely remarkable.
experimental result(table 1 ~ 7)
the variation of each developing mice blood of table 1 GOT activity
Compare * * with matched group
p<0.01; Compare # with ephedrine group
p<0.05, ##
psame under <0.01()
the variation of each developing mice blood of table 2 GPT activity
the variation of each developing mice liver MDA content of table 3
the variation of each developing mice liver SOD vigor of table 4
the variation of each developing mice liver CAT vigor of table 5
the surface density value that table 6 Bax albumen is expressed in murine liver tissue
the surface density value that table 7 c-Fos expresses in murine liver tissue
Comprehensively above-mentioned, through to ephedrine addiction animal experiment, the present invention can obviously reduce glutamic oxaloacetic transaminase, GOT (GOT) and glutamate pyruvate transaminase (GPT) content and hepatic tissue malonaldehyde (MDA) content in ephedrine addiction animal blood (
p<0.05 or
p<0.01) (table 1,2,3), significantly promote superoxide dismutase in hepatic tissue cell (SOD) and catalase (CAT) active (
p<0.05 or
p<0.01) (table 4,5), obviously alleviate histiocyte degeneration, reduce the positive expression intensity (table 6,7) of hepatic tissue cell Bax albumen and c-Fos.And use safety, no dependence, without obvious toxic-side effects.
Claims (3)
- The Chinese medicine composition of 1.Yi Zhongkang ephedrine tissue injury, it is characterized in that: this Chinese medicine composition is made water preparation by Radix Bupleuri, Radix Salviae Miltiorrhizae, Fructus Jujubae, the preparation method of this water preparation is: get respectively Radix Bupleuri, Radix Salviae Miltiorrhizae, Fructus Jujubae Chinese herbal medicine 500 g, respectively add 5 times of amounts of water, soak 1 h, decoct 30 min, leach medicinal liquid standby, medicinal residues add 5 times of amounts of water again, decoct 30 min, leach medicinal liquid standby; After twice medicinal liquid merged, concentrate and be settled to 250 mL, make concentrated solution; Each concentrated solution is Radix Bupleuri 45% by weight, Radix Salviae Miltiorrhizae 45%, and the ratio of Fructus Jujubae 10% merges standby.
- The Chinese medicine composition of 2.Yi Zhongkang ephedrine tissue injury, is characterized in that: this Chinese medicine composition becomes capsule by Radix Bupleuri, Radix Salviae Miltiorrhizae, red jujube; The weight proportion of its each component is: Radix Bupleuri 40-50%, Radix Salviae Miltiorrhizae 40-50%, Fructus Jujubae 5-15%.
- 3. the Chinese medicine composition of a kind of anti-ephedrine according to claim 2 tissue injury, is characterized in that: the weight proportion of described capsule is: Radix Bupleuri 45%, Radix Salviae Miltiorrhizae 45%, Fructus Jujubae 10%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210153013.3A CN102641349B (en) | 2012-05-17 | 2012-05-17 | Traditional Chinese medicine composite for resisting ephedrine damage and production technology thereof |
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| CN201210153013.3A CN102641349B (en) | 2012-05-17 | 2012-05-17 | Traditional Chinese medicine composite for resisting ephedrine damage and production technology thereof |
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| CN102641349B true CN102641349B (en) | 2014-02-05 |
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| CN105878704A (en) * | 2016-06-22 | 2016-08-24 | 西北师范大学 | Use of lily decoction in resisting ephedrine-induced lung tissue injury |
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| CN1256923A (en) * | 2000-01-12 | 2000-06-21 | 左志中 | Traditional Chinese medicinal composition for stopping drug taking and preparation process thereof |
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| CN1403109A (en) * | 2001-08-30 | 2003-03-19 | 张晋玮 | Recipe and prepn of medicine for stopping drug addiction |
| WO2003059367A1 (en) * | 2002-01-15 | 2003-07-24 | Chuanzheng Sun | A healthful chinese traditional medicinal preparation optimizing the brain |
| CN1621080A (en) * | 2003-11-28 | 2005-06-01 | 罗建会 | Medicine for abstaining from drug addiction and its preparation |
| CN101091788A (en) * | 2007-04-06 | 2007-12-26 | 叶树原 | Oral liquid for stopping drug addiction |
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2012
- 2012-05-17 CN CN201210153013.3A patent/CN102641349B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1144663A (en) * | 1995-03-03 | 1997-03-12 | 徐福才 | Detoxifying recovery pill and its preparation method |
| CN1242208A (en) * | 1998-07-17 | 2000-01-26 | 郑永江 | Special efficacy drug abstinence medicine |
| CN1256923A (en) * | 2000-01-12 | 2000-06-21 | 左志中 | Traditional Chinese medicinal composition for stopping drug taking and preparation process thereof |
| CN1403109A (en) * | 2001-08-30 | 2003-03-19 | 张晋玮 | Recipe and prepn of medicine for stopping drug addiction |
| CN1357359A (en) * | 2001-12-03 | 2002-07-10 | 中国人民解放军第一军医大学 | Drug addiction stopping machine medicine and its prepn |
| WO2003059367A1 (en) * | 2002-01-15 | 2003-07-24 | Chuanzheng Sun | A healthful chinese traditional medicinal preparation optimizing the brain |
| CN1621080A (en) * | 2003-11-28 | 2005-06-01 | 罗建会 | Medicine for abstaining from drug addiction and its preparation |
| CN101091788A (en) * | 2007-04-06 | 2007-12-26 | 叶树原 | Oral liquid for stopping drug addiction |
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